Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88539.t1 | KEH17601.1 | 71.9 | 64 | 18 | 0 | 1 | 64 | 36 | 99 | 1.00E-18 | 102.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88539.t1 | A0A072TK82 | 71.9 | 64 | 18 | 0 | 1 | 64 | 36 | 99 | 7.4e-19 | 102.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene88539 | 0.840033 | 1.11E-57 | -1.69E-46 |
| MS.gene049853 | MS.gene88539 | 0.854937 | 8.82E-62 | -1.69E-46 |
| MS.gene050430 | MS.gene88539 | 0.814983 | 1.17E-51 | -1.69E-46 |
| MS.gene052447 | MS.gene88539 | 0.833561 | 4.97E-56 | -1.69E-46 |
| MS.gene05265 | MS.gene88539 | 0.803852 | 2.86E-49 | -1.69E-46 |
| MS.gene05329 | MS.gene88539 | 0.819272 | 1.27E-52 | -1.69E-46 |
| MS.gene054174 | MS.gene88539 | 0.84995 | 2.33E-60 | -1.69E-46 |
| MS.gene054175 | MS.gene88539 | 0.838942 | 2.13E-57 | -1.69E-46 |
| MS.gene05435 | MS.gene88539 | 0.803576 | 3.26E-49 | -1.69E-46 |
| MS.gene054484 | MS.gene88539 | 0.820018 | 8.56E-53 | -1.69E-46 |
| MS.gene055017 | MS.gene88539 | 0.812401 | 4.32E-51 | -1.69E-46 |
| MS.gene055056 | MS.gene88539 | 0.809597 | 1.75E-50 | -1.69E-46 |
| MS.gene055058 | MS.gene88539 | 0.845299 | 4.43E-59 | -1.69E-46 |
| MS.gene05531 | MS.gene88539 | 0.806912 | 6.53E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene88539 | 0.816912 | 4.33E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene88539 | 0.826039 | 3.38E-54 | -1.69E-46 |
| MS.gene05925 | MS.gene88539 | 0.832179 | 1.10E-55 | -1.69E-46 |
| MS.gene059276 | MS.gene88539 | 0.844372 | 7.87E-59 | -1.69E-46 |
| MS.gene059458 | MS.gene88539 | 0.820345 | 7.20E-53 | -1.69E-46 |
| MS.gene059460 | MS.gene88539 | 0.806627 | 7.50E-50 | -1.69E-46 |
| MS.gene059584 | MS.gene88539 | 0.802564 | 5.29E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88539.t1 | MTR_1g037260 | 76.190 | 63 | 15 | 0 | 1 | 63 | 364 | 426 | 1.60e-25 | 98.2 |
| MS.gene88539.t1 | MTR_0006s0260 | 71.875 | 64 | 18 | 0 | 1 | 64 | 36 | 99 | 4.23e-25 | 92.4 |
| MS.gene88539.t1 | MTR_1g036840 | 70.312 | 64 | 19 | 0 | 1 | 64 | 158 | 221 | 1.08e-24 | 93.6 |
| MS.gene88539.t1 | MTR_1g036780 | 50.538 | 93 | 11 | 1 | 28 | 85 | 60 | 152 | 1.38e-19 | 77.4 |
| MS.gene88539.t1 | MTR_1g037080 | 51.000 | 100 | 27 | 1 | 8 | 85 | 125 | 224 | 9.00e-19 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTGCAGAGAAGTATTTA+AGG | 0.199010 | 1.4:+22474885 | MS.gene88539:CDS |
| CTGAGTGTTGGGTATGTTCT+TGG | 0.270844 | 1.4:+22474594 | MS.gene88539:CDS |
| ATTCAGTGTTTCTGCCGTTT+TGG | 0.291829 | 1.4:+22474854 | MS.gene88539:intron |
| ATCGTTGGATACGCGGATTC+GGG | 0.332937 | 1.4:+22474534 | MS.gene88539:CDS |
| AAATGGACGGGAAGAGGTTA+AGG | 0.336166 | 1.4:+22474484 | None:intergenic |
| GTTCTTGGTTGTTTGTTGTC+TGG | 0.357915 | 1.4:+22474609 | MS.gene88539:CDS |
| TATCGTTGGATACGCGGATT+CGG | 0.364368 | 1.4:+22474533 | MS.gene88539:CDS |
| ATTTAAGGATTATGAAAGTT+CGG | 0.440134 | 1.4:+22474900 | MS.gene88539:CDS |
| TGGTTGTTTGTTGTCTGGTA+AGG | 0.448767 | 1.4:+22474614 | MS.gene88539:CDS |
| TCGAAGAAATGCTGAGTGTT+GGG | 0.448826 | 1.4:+22474583 | MS.gene88539:CDS |
| ACTGAGAAGCTCTGTGAGAA+TGG | 0.462296 | 1.4:+22474639 | MS.gene88539:CDS |
| TGAGGGAAATGGACGGGAAG+AGG | 0.491779 | 1.4:+22474478 | None:intergenic |
| CAGCTTCAACCTTTCCAAAA+CGG | 0.492234 | 1.4:-22474868 | None:intergenic |
| CGGGTAGGTTGGAACAGTGT+TGG | 0.498978 | 1.4:+22474553 | MS.gene88539:CDS |
| GATTGATGAGGGAAATGGAC+GGG | 0.522479 | 1.4:+22474472 | None:intergenic |
| TGAGAATGGAGAAGTGGAGA+AGG | 0.524457 | 1.4:+22474653 | MS.gene88539:CDS |
| ATACGCGGATTCGGGTAGGT+TGG | 0.527066 | 1.4:+22474542 | MS.gene88539:CDS |
| TTCGAAGAAATGCTGAGTGT+TGG | 0.534324 | 1.4:+22474582 | MS.gene88539:CDS |
| AATGGACGGGAAGAGGTTAA+GGG | 0.535024 | 1.4:+22474485 | None:intergenic |
| ACTTATGAATGTGTTATCGT+TGG | 0.553017 | 1.4:+22474519 | MS.gene88539:CDS |
| ATGTGTTATCGTTGGATACG+CGG | 0.561073 | 1.4:+22474527 | MS.gene88539:CDS |
| TTGGATACGCGGATTCGGGT+AGG | 0.567623 | 1.4:+22474538 | MS.gene88539:CDS |
| GCTCTGTGAGAATGGAGAAG+TGG | 0.572425 | 1.4:+22474647 | MS.gene88539:CDS |
| GGATTGATGAGGGAAATGGA+CGG | 0.599624 | 1.4:+22474471 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTAAGGATTATGAAAGTT+CGG | + | chr1.4:22474900-22474919 | MS.gene88539:CDS | 20.0% |
| ! | ACTTATTCACATTTGATACA+TGG | + | chr1.4:22474731-22474750 | MS.gene88539:intron | 25.0% |
| AATCCTTTATCTAACAACAC+AGG | - | chr1.4:22474694-22474713 | None:intergenic | 30.0% | |
| ACTTATGAATGTGTTATCGT+TGG | + | chr1.4:22474519-22474538 | MS.gene88539:CDS | 30.0% | |
| ATCCTTTATCTAACAACACA+GGG | - | chr1.4:22474693-22474712 | None:intergenic | 30.0% | |
| ACAGTATAAGTCTATGTGTC+TGG | + | chr1.4:22474809-22474828 | MS.gene88539:intron | 35.0% | |
| ATAAGTCTATGTGTCTGGAT+TGG | + | chr1.4:22474814-22474833 | MS.gene88539:intron | 35.0% | |
| CATGGTTAATTATGCGAGAA+AGG | + | chr1.4:22474749-22474768 | MS.gene88539:intron | 35.0% | |
| !! | AAGCTGCAGAGAAGTATTTA+AGG | + | chr1.4:22474885-22474904 | MS.gene88539:CDS | 35.0% |
| ATGTGTTATCGTTGGATACG+CGG | + | chr1.4:22474527-22474546 | MS.gene88539:CDS | 40.0% | |
| CAGCTTCAACCTTTCCAAAA+CGG | - | chr1.4:22474871-22474890 | None:intergenic | 40.0% | |
| TCGAAGAAATGCTGAGTGTT+GGG | + | chr1.4:22474583-22474602 | MS.gene88539:CDS | 40.0% | |
| TTCGAAGAAATGCTGAGTGT+TGG | + | chr1.4:22474582-22474601 | MS.gene88539:CDS | 40.0% | |
| ! | TGCCCTGTGTTGTTAGATAA+AGG | + | chr1.4:22474688-22474707 | MS.gene88539:intron | 40.0% |
| !! | ATTCAGTGTTTCTGCCGTTT+TGG | + | chr1.4:22474854-22474873 | MS.gene88539:intron | 40.0% |
| !! | GTTCTTGGTTGTTTGTTGTC+TGG | + | chr1.4:22474609-22474628 | MS.gene88539:CDS | 40.0% |
| !! | TGGTTGTTTGTTGTCTGGTA+AGG | + | chr1.4:22474614-22474633 | MS.gene88539:CDS | 40.0% |
| ACTGAGAAGCTCTGTGAGAA+TGG | + | chr1.4:22474639-22474658 | MS.gene88539:CDS | 45.0% | |
| TATCGTTGGATACGCGGATT+CGG | + | chr1.4:22474533-22474552 | MS.gene88539:CDS | 45.0% | |
| TGAGAATGGAGAAGTGGAGA+AGG | + | chr1.4:22474653-22474672 | MS.gene88539:CDS | 45.0% | |
| !! | CTGAGTGTTGGGTATGTTCT+TGG | + | chr1.4:22474594-22474613 | MS.gene88539:CDS | 45.0% |
| !!! | GTGTTTCTGCCGTTTTGGAA+AGG | + | chr1.4:22474859-22474878 | MS.gene88539:intron | 45.0% |
| ATCGTTGGATACGCGGATTC+GGG | + | chr1.4:22474534-22474553 | MS.gene88539:CDS | 50.0% | |
| GCGAGAAAGGACGAAGTTCA+AGG | + | chr1.4:22474762-22474781 | MS.gene88539:intron | 50.0% | |
| GCTCTGTGAGAATGGAGAAG+TGG | + | chr1.4:22474647-22474666 | MS.gene88539:CDS | 50.0% | |
| ATACGCGGATTCGGGTAGGT+TGG | + | chr1.4:22474542-22474561 | MS.gene88539:CDS | 55.0% | |
| TTGGATACGCGGATTCGGGT+AGG | + | chr1.4:22474538-22474557 | MS.gene88539:CDS | 55.0% | |
| ! | CGGGTAGGTTGGAACAGTGT+TGG | + | chr1.4:22474553-22474572 | MS.gene88539:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 22474486 | 22474928 | 22474486 | ID=MS.gene88539 |
| chr1.4 | mRNA | 22474486 | 22474928 | 22474486 | ID=MS.gene88539.t1;Parent=MS.gene88539 |
| chr1.4 | exon | 22474486 | 22474674 | 22474486 | ID=MS.gene88539.t1.exon1;Parent=MS.gene88539.t1 |
| chr1.4 | CDS | 22474486 | 22474674 | 22474486 | ID=cds.MS.gene88539.t1;Parent=MS.gene88539.t1 |
| chr1.4 | exon | 22474860 | 22474928 | 22474860 | ID=MS.gene88539.t1.exon2;Parent=MS.gene88539.t1 |
| chr1.4 | CDS | 22474860 | 22474928 | 22474860 | ID=cds.MS.gene88539.t1;Parent=MS.gene88539.t1 |
| Gene Sequence |
| Protein sequence |
