AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88785


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88785.t1 MTR_5g070210 83.824 136 22 0 15 150 14 149 1.71e-80 240
MS.gene88785.t1 MTR_5g070230 55.652 115 49 1 39 151 3 117 1.09e-41 137
MS.gene88785.t1 MTR_5g070270 47.458 118 40 8 3 109 10 116 1.50e-18 76.6
MS.gene88785.t1 MTR_8g073540 49.296 71 35 1 74 143 28 98 1.01e-15 73.2
MS.gene88785.t1 MTR_1g095500 47.887 71 36 1 74 143 28 98 1.46e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88785.t1 AT5G58770 70.667 75 22 0 76 150 74 148 9.24e-33 118
MS.gene88785.t1 AT5G58780 56.790 81 35 0 72 152 63 143 1.07e-24 97.1
MS.gene88785.t1 AT5G58782 52.941 85 40 0 68 152 46 130 3.52e-24 95.1
MS.gene88785.t1 AT5G58782 52.941 85 40 0 68 152 64 148 4.60e-24 95.1
MS.gene88785.t1 AT2G23400 41.228 114 66 1 39 152 31 143 2.27e-23 91.3
MS.gene88785.t1 AT2G23410 51.899 79 38 0 74 152 65 143 1.56e-22 91.3
MS.gene88785.t1 AT2G23400 41.228 114 66 1 39 152 31 143 1.63e-22 90.9
MS.gene88785.t1 AT5G58784 49.315 73 37 0 78 150 69 141 2.46e-19 82.4
MS.gene88785.t1 AT2G17570 50.820 61 30 0 79 139 39 99 1.63e-15 72.0

Find 50 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATTCTCAACCGATAAT+TGG 0.274745 5.4:-55178770 MS.gene88785:CDS
TAAGCATGTGGCGGTGATTA+TGG 0.306230 5.4:-55178919 MS.gene88785:CDS
GTGTTATAGTTGGGGAATAA+AGG 0.324367 5.4:-55178805 MS.gene88785:CDS
CTCATTCTCGATATTCTTCA+AGG 0.331892 5.4:-55179067 MS.gene88785:CDS
GTGGTAGTAGTAGTCATCTT+TGG 0.377306 5.4:+55179125 None:intergenic
TTCAGAAATGGAGCCATTGC+CGG 0.378511 5.4:-55178967 MS.gene88785:CDS
ATGGGAGGTGGGCAAAGATG+AGG 0.382978 5.4:-55178890 MS.gene88785:CDS
GGTGAGGTTGTGTTATAGTT+GGG 0.400487 5.4:-55178814 MS.gene88785:CDS
TACCACTAACCACCCTCATC+GGG 0.419799 5.4:-55179108 MS.gene88785:CDS
CTTAGGTCGAATCCAATTAT+CGG 0.425110 5.4:+55178758 None:intergenic
TGGTGAGGTTGTGTTATAGT+TGG 0.451686 5.4:-55178815 MS.gene88785:CDS
CGGTGATTATGGACGGGAAT+GGG 0.455731 5.4:-55178908 MS.gene88785:CDS
TGGGAGGTGGGCAAAGATGA+GGG 0.455809 5.4:-55178889 MS.gene88785:CDS
GGGAGGTGGGCAAAGATGAG+GGG 0.456021 5.4:-55178888 MS.gene88785:CDS
GCTTGCAGTTCCTCCGGCAA+TGG 0.479704 5.4:+55178954 None:intergenic
GGAGGTGGGCAAAGATGAGG+GGG 0.479875 5.4:-55178887 MS.gene88785:CDS
CGATAATTGGATTCGACCTA+AGG 0.480618 5.4:-55178757 MS.gene88785:CDS
AGTGAAATTAAAATGACCTT+AGG 0.481788 5.4:+55178741 None:intergenic
CCGTCTGCGGGTCATGTAGC+CGG 0.489572 5.4:-55178858 MS.gene88785:CDS
TTATGGACGGGAATGGGAGG+TGG 0.493196 5.4:-55178902 MS.gene88785:CDS
TGCGGCAATAGTGACGCGTT+TGG 0.497199 5.4:+55179037 None:intergenic
AGCTCCGCTTGCAGTTCCTC+CGG 0.507703 5.4:+55178948 None:intergenic
GCGGTGATTATGGACGGGAA+TGG 0.517607 5.4:-55178909 MS.gene88785:CDS
CTACCACTAACCACCCTCAT+CGG 0.530891 5.4:-55179109 MS.gene88785:CDS
CGGCTACATGACCCGCAGAC+GGG 0.532986 5.4:+55178859 None:intergenic
AAGAGTTCTATCTAGTGGCA+TGG 0.545374 5.4:+55179160 None:intergenic
GTGAGGTTGTGTTATAGTTG+GGG 0.555675 5.4:-55178813 MS.gene88785:CDS
ATAATCACCGCCACATGCTT+AGG 0.560502 5.4:+55178921 None:intergenic
TATGGACGGGAATGGGAGGT+GGG 0.565954 5.4:-55178901 MS.gene88785:CDS
AGAGTTCTATCTAGTGGCAT+GGG 0.571631 5.4:+55179161 None:intergenic
ATGTGGCGGTGATTATGGAC+GGG 0.576395 5.4:-55178914 MS.gene88785:CDS
TGTAAAGTCGTTGAGAAGGA+TGG 0.579930 5.4:-55178835 MS.gene88785:CDS
GGCTACATGACCCGCAGACG+GGG 0.586275 5.4:+55178860 None:intergenic
CATGTGGCGGTGATTATGGA+CGG 0.587809 5.4:-55178915 MS.gene88785:CDS
GTTATAAGAGTTCTATCTAG+TGG 0.591644 5.4:+55179155 None:intergenic
ATTGCCGGAGGAACTGCAAG+CGG 0.596025 5.4:-55178952 MS.gene88785:CDS
GTAGTAGTAGTCATCTTTGG+TGG 0.604592 5.4:+55179128 None:intergenic
TGATTATGGACGGGAATGGG+AGG 0.615382 5.4:-55178905 MS.gene88785:CDS
CCGGCTACATGACCCGCAGA+CGG 0.616650 5.4:+55178858 None:intergenic
CCGGTGTAAAGTCGTTGAGA+AGG 0.624155 5.4:-55178839 MS.gene88785:CDS
AGTCGTTGAGAAGGATGGTG+AGG 0.625194 5.4:-55178830 MS.gene88785:CDS
GGAGCTGATGCCTAAGCATG+TGG 0.626089 5.4:-55178931 MS.gene88785:CDS
AGAAATGGAGCCATTGCCGG+AGG 0.635367 5.4:-55178964 MS.gene88785:CDS
GGTGGATGGGGAAACTTCCG+TGG 0.636917 5.4:-55179000 MS.gene88785:CDS
CCTTCTCAACGACTTTACAC+CGG 0.638232 5.4:+55178839 None:intergenic
CCACTAACCACCCTCATCGG+GGG 0.665764 5.4:-55179106 MS.gene88785:CDS
GCTACATGACCCGCAGACGG+GGG 0.669075 5.4:+55178861 None:intergenic
ACCACTAACCACCCTCATCG+GGG 0.670600 5.4:-55179107 MS.gene88785:CDS
CACCTGATCCTGAAAAGCTG+CGG 0.703606 5.4:+55179019 None:intergenic
GCTGATGCCTAAGCATGTGG+CGG 0.712093 5.4:-55178928 MS.gene88785:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTGAAATTAAAATGACCTT+AGG + chr5.4:55179136-55179155 None:intergenic 25.0%
GTTATAAGAGTTCTATCTAG+TGG + chr5.4:55178722-55178741 None:intergenic 30.0%
CTCATTCTCGATATTCTTCA+AGG - chr5.4:55178807-55178826 MS.gene88785:CDS 35.0%
CTTAGGTCGAATCCAATTAT+CGG + chr5.4:55179119-55179138 None:intergenic 35.0%
GTGTTATAGTTGGGGAATAA+AGG - chr5.4:55179069-55179088 MS.gene88785:CDS 35.0%
TTGCATTCTCAACCGATAAT+TGG - chr5.4:55179104-55179123 MS.gene88785:CDS 35.0%
! TTCTGAAAAATCTTCGTCCA+CGG + chr5.4:55178894-55178913 None:intergenic 35.0%
!! GGACGAAGATTTTTCAGAAA+TGG - chr5.4:55178895-55178914 MS.gene88785:CDS 35.0%
CGATAATTGGATTCGACCTA+AGG - chr5.4:55179117-55179136 MS.gene88785:CDS 40.0%
GGTGAGGTTGTGTTATAGTT+GGG - chr5.4:55179060-55179079 MS.gene88785:CDS 40.0%
GTAGTAGTAGTCATCTTTGG+TGG + chr5.4:55178749-55178768 None:intergenic 40.0%
GTGAGGTTGTGTTATAGTTG+GGG - chr5.4:55179061-55179080 MS.gene88785:CDS 40.0%
GTGGTAGTAGTAGTCATCTT+TGG + chr5.4:55178752-55178771 None:intergenic 40.0%
TGGTGAGGTTGTGTTATAGT+TGG - chr5.4:55179059-55179078 MS.gene88785:CDS 40.0%
TGTAAAGTCGTTGAGAAGGA+TGG - chr5.4:55179039-55179058 MS.gene88785:CDS 40.0%
ATAATCACCGCCACATGCTT+AGG + chr5.4:55178956-55178975 None:intergenic 45.0%
CCTTCTCAACGACTTTACAC+CGG + chr5.4:55179038-55179057 None:intergenic 45.0%
TAAGCATGTGGCGGTGATTA+TGG - chr5.4:55178955-55178974 MS.gene88785:CDS 45.0%
TTCAGAAATGGAGCCATTGC+CGG - chr5.4:55178907-55178926 MS.gene88785:CDS 45.0%
!! CTTTTCAGGATCAGGTGGAT+GGG - chr5.4:55178861-55178880 MS.gene88785:CDS 45.0%
!! TTTTCAGGATCAGGTGGATG+GGG - chr5.4:55178862-55178881 MS.gene88785:CDS 45.0%
AGTCGTTGAGAAGGATGGTG+AGG - chr5.4:55179044-55179063 MS.gene88785:CDS 50.0%
ATGTGGCGGTGATTATGGAC+GGG - chr5.4:55178960-55178979 MS.gene88785:CDS 50.0%
CACCTGATCCTGAAAAGCTG+CGG + chr5.4:55178858-55178877 None:intergenic 50.0%
CATGTGGCGGTGATTATGGA+CGG - chr5.4:55178959-55178978 MS.gene88785:CDS 50.0%
CCGGTGTAAAGTCGTTGAGA+AGG - chr5.4:55179035-55179054 MS.gene88785:CDS 50.0%
CGGTGATTATGGACGGGAAT+GGG - chr5.4:55178966-55178985 MS.gene88785:CDS 50.0%
CTACCACTAACCACCCTCAT+CGG - chr5.4:55178765-55178784 MS.gene88785:CDS 50.0%
TACCACTAACCACCCTCATC+GGG - chr5.4:55178766-55178785 MS.gene88785:CDS 50.0%
TGATTATGGACGGGAATGGG+AGG - chr5.4:55178969-55178988 MS.gene88785:CDS 50.0%
! CACTATTGCCGCAGCTTTTC+AGG - chr5.4:55178847-55178866 MS.gene88785:CDS 50.0%
!! GCTTTTCAGGATCAGGTGGA+TGG - chr5.4:55178860-55178879 MS.gene88785:CDS 50.0%
ACCACTAACCACCCTCATCG+GGG - chr5.4:55178767-55178786 MS.gene88785:CDS 55.0%
AGAAATGGAGCCATTGCCGG+AGG - chr5.4:55178910-55178929 MS.gene88785:CDS 55.0%
ATCTGAGTGATCCCCCGATG+AGG + chr5.4:55178782-55178801 None:intergenic 55.0%
ATGGGAGGTGGGCAAAGATG+AGG - chr5.4:55178984-55179003 MS.gene88785:CDS 55.0%
ATTGCCGGAGGAACTGCAAG+CGG - chr5.4:55178922-55178941 MS.gene88785:CDS 55.0%
GCGGTGATTATGGACGGGAA+TGG - chr5.4:55178965-55178984 MS.gene88785:CDS 55.0%
GCTGATGCCTAAGCATGTGG+CGG - chr5.4:55178946-55178965 MS.gene88785:CDS 55.0%
GGAGCTGATGCCTAAGCATG+TGG - chr5.4:55178943-55178962 MS.gene88785:CDS 55.0%
TATGGACGGGAATGGGAGGT+GGG - chr5.4:55178973-55178992 MS.gene88785:CDS 55.0%
TCTGAGTGATCCCCCGATGA+GGG + chr5.4:55178781-55178800 None:intergenic 55.0%
TGCGGCAATAGTGACGCGTT+TGG + chr5.4:55178840-55178859 None:intergenic 55.0%
TGGGAGGTGGGCAAAGATGA+GGG - chr5.4:55178985-55179004 MS.gene88785:CDS 55.0%
TTATGGACGGGAATGGGAGG+TGG - chr5.4:55178972-55178991 MS.gene88785:CDS 55.0%
!! CGCAGCTTTTCAGGATCAGG+TGG - chr5.4:55178856-55178875 MS.gene88785:CDS 55.0%
!! TGCCGCAGCTTTTCAGGATC+AGG - chr5.4:55178853-55178872 MS.gene88785:CDS 55.0%
AGCTCCGCTTGCAGTTCCTC+CGG + chr5.4:55178929-55178948 None:intergenic 60.0%
CCACTAACCACCCTCATCGG+GGG - chr5.4:55178768-55178787 MS.gene88785:CDS 60.0%
GCTTGCAGTTCCTCCGGCAA+TGG + chr5.4:55178923-55178942 None:intergenic 60.0%
GGAGGTGGGCAAAGATGAGG+GGG - chr5.4:55178987-55179006 MS.gene88785:CDS 60.0%
GGGAGGTGGGCAAAGATGAG+GGG - chr5.4:55178986-55179005 MS.gene88785:CDS 60.0%
GGTGGATGGGGAAACTTCCG+TGG - chr5.4:55178874-55178893 MS.gene88785:CDS 60.0%
CCCCCGATGAGGGTGGTTAG+TGG + chr5.4:55178771-55178790 None:intergenic 65.0%
CCGGCTACATGACCCGCAGA+CGG + chr5.4:55179019-55179038 None:intergenic 65.0%
CCGTCTGCGGGTCATGTAGC+CGG - chr5.4:55179016-55179035 MS.gene88785:CDS 65.0%
CGGCTACATGACCCGCAGAC+GGG + chr5.4:55179018-55179037 None:intergenic 65.0%
GAGTGATCCCCCGATGAGGG+TGG + chr5.4:55178778-55178797 None:intergenic 65.0%
GCTACATGACCCGCAGACGG+GGG + chr5.4:55179016-55179035 None:intergenic 65.0%
GGCTACATGACCCGCAGACG+GGG + chr5.4:55179017-55179036 None:intergenic 65.0%
AGGGGGTTACCCCCGTCTGC+GGG - chr5.4:55179004-55179023 MS.gene88785:CDS 70.0%
GAGGGGGTTACCCCCGTCTG+CGG - chr5.4:55179003-55179022 MS.gene88785:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 55178716 55179180 55178716 ID=MS.gene88785
chr5.4 mRNA 55178716 55179180 55178716 ID=MS.gene88785.t1;Parent=MS.gene88785
chr5.4 exon 55178716 55179180 55178716 ID=MS.gene88785.t1.exon1;Parent=MS.gene88785.t1
chr5.4 CDS 55178716 55179180 55178716 ID=cds.MS.gene88785.t1;Parent=MS.gene88785.t1
Gene Sequence

>MS.gene88785

ATGCCACTAGATAGAACTCTTATAACTTGTCCACCAAAGATGACTACTACTACCACTAACCACCCTCATCGGGGGATCACTCAGATACAAACTCATTCTCGATATTCTTCAAGGTTTGTTACCAAACGCGTCACTATTGCCGCAGCTTTTCAGGATCAGGTGGATGGGGAAACTTCCGTGGACGAAGATTTTTCAGAAATGGAGCCATTGCCGGAGGAACTGCAAGCGGAGCTGATGCCTAAGCATGTGGCGGTGATTATGGACGGGAATGGGAGGTGGGCAAAGATGAGGGGGTTACCCCCGTCTGCGGGTCATGTAGCCGGTGTAAAGTCGTTGAGAAGGATGGTGAGGTTGTGTTATAGTTGGGGAATAAAGGTTCTAACTGTTTTTGCATTCTCAACCGATAATTGGATTCGACCTAAGGTCATTTTAATTTCACTTGTCAGTTTAGTTAACTTTGATTAA

Protein sequence

>MS.gene88785.t1

MPLDRTLITCPPKMTTTTTNHPHRGITQIQTHSRYSSRFVTKRVTIAAAFQDQVDGETSVDEDFSEMEPLPEELQAELMPKHVAVIMDGNGRWAKMRGLPPSAGHVAGVKSLRRMVRLCYSWGIKVLTVFAFSTDNWIRPKVILISLVSLVNFD