AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90176


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90176.t1 MTR_4g094232 92.056 214 14 1 1 214 1 211 1.75e-99 308
MS.gene90176.t1 MTR_7g116990 58.974 78 29 1 140 214 82 159 1.93e-25 97.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90176.t1 AT4G33520 71.739 92 26 0 123 214 133 224 7.62e-40 137
MS.gene90176.t1 AT4G33520 71.739 92 26 0 123 214 133 224 7.99e-40 136
MS.gene90176.t1 AT4G33520 71.739 92 26 0 123 214 133 224 7.58e-37 136
MS.gene90176.t1 AT4G33520 71.739 92 26 0 123 214 133 224 8.11e-37 136

Find 64 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAATTGGCAAAAGCAATT+AGG 0.201723 4.2:-19252636 MS.gene90176:CDS
TCGAGCTCCGCTGCTTCTTT+TGG 0.239668 4.2:-19253251 MS.gene90176:CDS
GGAGGTGGTGGTAGTGGTTC+TGG 0.249231 4.2:-19253179 MS.gene90176:CDS
TCTTCTGCCGGCGGTGGAAA+TGG 0.342614 4.2:-19253227 MS.gene90176:CDS
TGTTATCATTCTCGATGTTT+CGG 0.361835 4.2:-19253084 MS.gene90176:intron
AGATGCTGCGCAAGAGGTTT+CGG 0.362319 4.2:-19253120 MS.gene90176:CDS
CAAATTGGCAAAAGCAATTA+GGG 0.366216 4.2:-19252635 MS.gene90176:CDS
AGTGGTTCTGGTGGTGAGTC+TGG 0.377787 4.2:-19253167 MS.gene90176:CDS
TAACAGGGCGTTTGAGGTTG+TGG 0.389375 4.2:+19253399 None:intergenic
TGGAGATGGTAGTGTAAAAT+TGG 0.390644 4.2:-19253147 MS.gene90176:CDS
GGAGGTGCTGGAGTTGGAAA+TGG 0.392164 4.2:-19253206 MS.gene90176:CDS
GGCGTTTGAGGTTGTGGCGG+AGG 0.403025 4.2:+19253405 None:intergenic
CAACAGAGACAGCAATAGTT+TGG 0.405470 4.2:-19252683 MS.gene90176:CDS
GAGTGTGCGGTGAGGTGCTC+CGG 0.405543 4.2:+19253427 None:intergenic
CGGTTGTTGTAGGAAGTAAC+AGG 0.413393 4.2:+19253383 None:intergenic
GGAGGAGGAGGTGGTGGTAG+TGG 0.418009 4.2:-19253185 MS.gene90176:CDS
GGAAATGGAGGAGGAGGTGG+TGG 0.421409 4.2:-19253191 MS.gene90176:CDS
GGCGGTGGAAATGGAGGTGC+TGG 0.439969 4.2:-19253218 MS.gene90176:CDS
TTTGGTTCTTCTGCCGGCGG+TGG 0.441304 4.2:-19253233 MS.gene90176:CDS
AGCTCGAAACGCAGCGCGTA+CGG 0.449637 4.2:+19253267 None:intergenic
AGGAAGTAACAGGGCGTTTG+AGG 0.450260 4.2:+19253393 None:intergenic
GTTGGAAATGGAGGAGGAGG+TGG 0.455716 4.2:-19253194 MS.gene90176:CDS
TTACGGAGTGATGGCGATAA+CGG 0.456860 4.2:+19253320 None:intergenic
AGAGCAGCGCGCGGAAGTTA+CGG 0.458880 4.2:+19253303 None:intergenic
GCATTTCACCGTCATTTCTC+CGG 0.458962 4.2:-19253446 MS.gene90176:CDS
GATGGTAGTGTAAAATTGGT+TGG 0.482307 4.2:-19253143 MS.gene90176:CDS
AATGCGGGAACGGTTGTTGT+AGG 0.484939 4.2:+19253373 None:intergenic
ACGGTTATCGCAGAATCCAT+TGG 0.500062 4.2:+19253500 None:intergenic
AGCACCTCCATTTCCACCGC+CGG 0.504429 4.2:+19253220 None:intergenic
AGCATCTGTGAAGAGGATTC+TGG 0.510233 4.2:-19252941 MS.gene90176:CDS
GGAAATGGAGGTGCTGGAGT+TGG 0.514880 4.2:-19253212 MS.gene90176:CDS
ACATCGAGAATGATAACATC+AGG 0.528838 4.2:+19253089 None:intergenic
CATCTGCGCCGTCGTGGTGA+CGG 0.530176 4.2:+19253481 None:intergenic
GCTGGAGTTGGAAATGGAGG+AGG 0.531934 4.2:-19253200 MS.gene90176:CDS
CGTAGAAGTACAAGAGGTAG+AGG 0.539542 4.2:+19253343 None:intergenic
GCAGAACATTTAACTAGCTG+TGG 0.547024 4.2:-19252603 MS.gene90176:CDS
GGTAGAGGAACACGGAATGC+GGG 0.552623 4.2:+19253358 None:intergenic
GGAGTTGGAAATGGAGGAGG+AGG 0.552972 4.2:-19253197 MS.gene90176:CDS
GCGCGGAAGTTACGGAGTGA+TGG 0.555469 4.2:+19253311 None:intergenic
GGTTGGAGATGCTGCGCAAG+AGG 0.555665 4.2:-19253126 MS.gene90176:CDS
TCTGCCGGCGGTGGAAATGG+AGG 0.568541 4.2:-19253224 MS.gene90176:CDS
GAAACGCAGCGCGTACGGCA+CGG 0.571618 4.2:+19253272 None:intergenic
AGAAGAACCAAAAGAAGCAG+CGG 0.577913 4.2:+19253244 None:intergenic
TGTTAGGGAATGGTGTGTGG+TGG 0.581724 4.2:-19252970 MS.gene90176:intron
GGTTTCAATTCTTGCCTCCG+TGG 0.583614 4.2:-19252582 None:intergenic
GGTGGTGGTAGTGGTTCTGG+TGG 0.594008 4.2:-19253176 MS.gene90176:CDS
GGTGCTGGAGTTGGAAATGG+AGG 0.596153 4.2:-19253203 MS.gene90176:CDS
TCTGGTGGTGAGTCTGGAGA+TGG 0.597845 4.2:-19253161 MS.gene90176:CDS
AGGTAGAGGAACACGGAATG+CGG 0.598468 4.2:+19253357 None:intergenic
CAGGGCGTTTGAGGTTGTGG+CGG 0.604275 4.2:+19253402 None:intergenic
AGGAACACGGAATGCGGGAA+CGG 0.604377 4.2:+19253363 None:intergenic
GTTTGTTAGGGAATGGTGTG+TGG 0.604721 4.2:-19252973 MS.gene90176:intron
GAGGTGCTCCGGAGAAATGA+CGG 0.610156 4.2:+19253438 None:intergenic
GGTTGTGGCGGAGGAGTGTG+CGG 0.647982 4.2:+19253414 None:intergenic
ACACTAGCAGATGACACTTG+TGG 0.655705 4.2:+19252714 None:intergenic
GTACAAGAGGTAGAGGAACA+CGG 0.659238 4.2:+19253350 None:intergenic
TGCGATAACCGTCACCACGA+CGG 0.665901 4.2:-19253489 MS.gene90176:CDS
TAACGGCGTAGAAGTACAAG+AGG 0.666502 4.2:+19253337 None:intergenic
GATGTGCAGCATCTGTGAAG+AGG 0.673377 4.2:-19252948 MS.gene90176:CDS
AAGTAGCATCTGCGCCGTCG+TGG 0.674934 4.2:+19253475 None:intergenic
GGTTGTTGTAGGAAGTAACA+GGG 0.716557 4.2:+19253384 None:intergenic
TGGCGGAGGAGTGTGCGGTG+AGG 0.719500 4.2:+19253419 None:intergenic
GTGTTCGTAAGAGCAGCGCG+CGG 0.723251 4.2:+19253294 None:intergenic
AGCTTGAAAATATACCACGG+AGG 0.776401 4.2:+19252568 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAAGTAAGTTTTTTATATT+TGG - chr4.2:19253166-19253185 MS.gene90176:CDS 15.0%
!!! CTTTCATTATCTTATTTCTA+CGG - chr4.2:19253216-19253235 MS.gene90176:CDS 20.0%
!!! TTTTGTTTGTGTGTTTGTTA+GGG - chr4.2:19253095-19253114 MS.gene90176:CDS 25.0%
CAAATTGGCAAAAGCAATTA+GGG - chr4.2:19253445-19253464 MS.gene90176:CDS 30.0%
TACGCTTAAGTAGTTGAAAT+GGG - chr4.2:19253276-19253295 MS.gene90176:CDS 30.0%
TGTTATCATTCTCGATGTTT+CGG - chr4.2:19252996-19253015 MS.gene90176:intron 30.0%
!!! GTTTTGTTTGTGTGTTTGTT+AGG - chr4.2:19253094-19253113 MS.gene90176:CDS 30.0%
AAGCAAAAACTGCACCAAAT+TGG - chr4.2:19253430-19253449 MS.gene90176:CDS 35.0%
ACATCGAGAATGATAACATC+AGG + chr4.2:19252994-19253013 None:intergenic 35.0%
CCAAATTGGCAAAAGCAATT+AGG - chr4.2:19253444-19253463 MS.gene90176:CDS 35.0%
GTACGCTTAAGTAGTTGAAA+TGG - chr4.2:19253275-19253294 MS.gene90176:CDS 35.0%
! GATGGTAGTGTAAAATTGGT+TGG - chr4.2:19252937-19252956 MS.gene90176:CDS 35.0%
! TGGAGATGGTAGTGTAAAAT+TGG - chr4.2:19252933-19252952 MS.gene90176:CDS 35.0%
! TTTGTGTGTTTGTTAGGGAA+TGG - chr4.2:19253100-19253119 MS.gene90176:CDS 35.0%
!! CCTAATTGCTTTTGCCAATT+TGG + chr4.2:19253447-19253466 None:intergenic 35.0%
AGAAGAACCAAAAGAAGCAG+CGG + chr4.2:19252839-19252858 None:intergenic 40.0%
CAACAGAGACAGCAATAGTT+TGG - chr4.2:19253397-19253416 MS.gene90176:CDS 40.0%
GCAGAACATTTAACTAGCTG+TGG - chr4.2:19253477-19253496 MS.gene90176:CDS 40.0%
GGTTGTTGTAGGAAGTAACA+GGG + chr4.2:19252699-19252718 None:intergenic 40.0%
! GCAGTTTTTGCTTCAGAAAC+AGG + chr4.2:19253423-19253442 None:intergenic 40.0%
ACACTAGCAGATGACACTTG+TGG + chr4.2:19253369-19253388 None:intergenic 45.0%
AGCATCTGTGAAGAGGATTC+TGG - chr4.2:19253139-19253158 MS.gene90176:CDS 45.0%
CGGTTGTTGTAGGAAGTAAC+AGG + chr4.2:19252700-19252719 None:intergenic 45.0%
GCATTTCACCGTCATTTCTC+CGG - chr4.2:19252634-19252653 MS.gene90176:CDS 45.0%
GTACAAGAGGTAGAGGAACA+CGG + chr4.2:19252733-19252752 None:intergenic 45.0%
! CGTAGAAGTACAAGAGGTAG+AGG + chr4.2:19252740-19252759 None:intergenic 45.0%
! GTTTGTTAGGGAATGGTGTG+TGG - chr4.2:19253107-19253126 MS.gene90176:CDS 45.0%
! TAACGGCGTAGAAGTACAAG+AGG + chr4.2:19252746-19252765 None:intergenic 45.0%
! TTACGGAGTGATGGCGATAA+CGG + chr4.2:19252763-19252782 None:intergenic 45.0%
!!! GCTTCTTTTGGTTCTTCTGC+CGG - chr4.2:19252841-19252860 MS.gene90176:intron 45.0%
AATGCGGGAACGGTTGTTGT+AGG + chr4.2:19252710-19252729 None:intergenic 50.0%
AGATGCTGCGCAAGAGGTTT+CGG - chr4.2:19252960-19252979 MS.gene90176:CDS 50.0%
AGGTAGAGGAACACGGAATG+CGG + chr4.2:19252726-19252745 None:intergenic 50.0%
GATGTGCAGCATCTGTGAAG+AGG - chr4.2:19253132-19253151 MS.gene90176:CDS 50.0%
TGTTAGGGAATGGTGTGTGG+TGG - chr4.2:19253110-19253129 MS.gene90176:CDS 50.0%
! AGGAAGTAACAGGGCGTTTG+AGG + chr4.2:19252690-19252709 None:intergenic 50.0%
! TAACAGGGCGTTTGAGGTTG+TGG + chr4.2:19252684-19252703 None:intergenic 50.0%
!!! TCTTTTGGTTCTTCTGCCGG+CGG - chr4.2:19252844-19252863 MS.gene90176:intron 50.0%
AGGAACACGGAATGCGGGAA+CGG + chr4.2:19252720-19252739 None:intergenic 55.0%
CAGATGACACTTGTGGCTGC+AGG + chr4.2:19253362-19253381 None:intergenic 55.0%
GCTGGAGTTGGAAATGGAGG+AGG - chr4.2:19252880-19252899 MS.gene90176:intron 55.0%
GGAGTTGGAAATGGAGGAGG+AGG - chr4.2:19252883-19252902 MS.gene90176:intron 55.0%
GGTAGAGGAACACGGAATGC+GGG + chr4.2:19252725-19252744 None:intergenic 55.0%
GTTGGAAATGGAGGAGGAGG+TGG - chr4.2:19252886-19252905 MS.gene90176:intron 55.0%
TGCGATAACCGTCACCACGA+CGG - chr4.2:19252591-19252610 MS.gene90176:CDS 55.0%
! GAGGTGCTCCGGAGAAATGA+CGG + chr4.2:19252645-19252664 None:intergenic 55.0%
! GGTGCTGGAGTTGGAAATGG+AGG - chr4.2:19252877-19252896 MS.gene90176:intron 55.0%
! TCGAGCTCCGCTGCTTCTTT+TGG - chr4.2:19252829-19252848 MS.gene90176:intron 55.0%
! TCTGGTGGTGAGTCTGGAGA+TGG - chr4.2:19252919-19252938 MS.gene90176:intron 55.0%
!! AGTGGTTCTGGTGGTGAGTC+TGG - chr4.2:19252913-19252932 MS.gene90176:intron 55.0%
!! GGAAATGGAGGTGCTGGAGT+TGG - chr4.2:19252868-19252887 MS.gene90176:intron 55.0%
!! GGAGGTGCTGGAGTTGGAAA+TGG - chr4.2:19252874-19252893 MS.gene90176:intron 55.0%
AAGTAGCATCTGCGCCGTCG+TGG + chr4.2:19252608-19252627 None:intergenic 60.0%
AGAGCAGCGCGCGGAAGTTA+CGG + chr4.2:19252780-19252799 None:intergenic 60.0%
AGCACCTCCATTTCCACCGC+CGG + chr4.2:19252863-19252882 None:intergenic 60.0%
AGCTCGAAACGCAGCGCGTA+CGG + chr4.2:19252816-19252835 None:intergenic 60.0%
GCGCGGAAGTTACGGAGTGA+TGG + chr4.2:19252772-19252791 None:intergenic 60.0%
GGAAATGGAGGAGGAGGTGG+TGG - chr4.2:19252889-19252908 MS.gene90176:intron 60.0%
GGTTGGAGATGCTGCGCAAG+AGG - chr4.2:19252954-19252973 MS.gene90176:CDS 60.0%
GTGTTCGTAAGAGCAGCGCG+CGG + chr4.2:19252789-19252808 None:intergenic 60.0%
TCTTCTGCCGGCGGTGGAAA+TGG - chr4.2:19252853-19252872 MS.gene90176:intron 60.0%
! CAGGGCGTTTGAGGTTGTGG+CGG + chr4.2:19252681-19252700 None:intergenic 60.0%
!! GGAGGTGGTGGTAGTGGTTC+TGG - chr4.2:19252901-19252920 MS.gene90176:intron 60.0%
!! GGTGGTGGTAGTGGTTCTGG+TGG - chr4.2:19252904-19252923 MS.gene90176:intron 60.0%
!! TTTGGTTCTTCTGCCGGCGG+TGG - chr4.2:19252847-19252866 MS.gene90176:intron 60.0%
CATCTGCGCCGTCGTGGTGA+CGG + chr4.2:19252602-19252621 None:intergenic 65.0%
GAAACGCAGCGCGTACGGCA+CGG + chr4.2:19252811-19252830 None:intergenic 65.0%
TCTGCCGGCGGTGGAAATGG+AGG - chr4.2:19252856-19252875 MS.gene90176:intron 65.0%
! GAGTGTGCGGTGAGGTGCTC+CGG + chr4.2:19252656-19252675 None:intergenic 65.0%
! GGAGGAGGAGGTGGTGGTAG+TGG - chr4.2:19252895-19252914 MS.gene90176:intron 65.0%
! GGCGGTGGAAATGGAGGTGC+TGG - chr4.2:19252862-19252881 MS.gene90176:intron 65.0%
! GGCGTTTGAGGTTGTGGCGG+AGG + chr4.2:19252678-19252697 None:intergenic 65.0%
! GGTTGTGGCGGAGGAGTGTG+CGG + chr4.2:19252669-19252688 None:intergenic 65.0%
! TGGCGGAGGAGTGTGCGGTG+AGG + chr4.2:19252664-19252683 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 19252583 19253519 19252583 ID=MS.gene90176
chr4.2 mRNA 19252583 19253519 19252583 ID=MS.gene90176.t1;Parent=MS.gene90176
chr4.2 exon 19253085 19253519 19253085 ID=MS.gene90176.t1.exon1;Parent=MS.gene90176.t1
chr4.2 CDS 19253085 19253519 19253085 ID=cds.MS.gene90176.t1;Parent=MS.gene90176.t1
chr4.2 exon 19252933 19252986 19252933 ID=MS.gene90176.t1.exon2;Parent=MS.gene90176.t1
chr4.2 CDS 19252933 19252986 19252933 ID=cds.MS.gene90176.t1;Parent=MS.gene90176.t1
chr4.2 exon 19252583 19252736 19252583 ID=MS.gene90176.t1.exon3;Parent=MS.gene90176.t1
chr4.2 CDS 19252583 19252736 19252583 ID=cds.MS.gene90176.t1;Parent=MS.gene90176.t1
Gene Sequence

>MS.gene90176

ATGGATTCTGCGATAACCGTCACCACGACGGCGCAGATGCTACTTTTCAGAGCATTTCACCGTCATTTCTCCGGAGCACCTCACCGCACACTCCTCCGCCACAACCTCAAACGCCCTGTTACTTCCTACAACAACCGTTCCCGCATTCCGTGTTCCTCTACCTCTTGTACTTCTACGCCGTTATCGCCATCACTCCGTAACTTCCGCGCGCTGCTCTTACGAACACCGTGCCGTACGCGCTGCGTTTCGAGCTCCGCTGCTTCTTTTGGTTCTTCTGCCGGCGGTGGAAATGGAGGTGCTGGAGTTGGAAATGGAGGAGGAGGTGGTGGTAGTGGTTCTGGTGGTGAGTCTGGAGATGGTAGTGTAAAATTGGTTGGAGATGCTGCGCAAGAGGTTTCGGCTCTTTCGCCTGATGTTATCATTCTCGATGTTTCGGTATGAACTCTTCACTCTGAGTTTTTCTTCGTTCGTATTGTTTTTCTTGCTTTTTCTTTTTAAAATTAATAATTCAGTTTTGTTTGTGTGTTTGTTAGGGAATGGTGTGTGGTGGATGTGCAGCATCTGTGAAGAGGATTCTGGAAAGTCAAGTAAGTTTTTTATATTTGGATTCTACTTGAATTTGATTAGTTGATGCTTTCATTATCTTATTTCTACGGTGATTAATGTAATGTTGAGCGAATGTTGCTATGTTTGTACGCTTAAGTAGTTGAAATGGGATAAGCATGCAGTATATTTAGTTTCATAAGTATTAATGATTAGTTTAATTTTTACACTAACCTGCAGCCACAAGTGTCATCTGCTAGTGTAAATTTAACAACAGAGACAGCAATAGTTTGGCCTGTTTCTGAAGCAAAAACTGCACCAAATTGGCAAAAGCAATTAGGGAAGACACTTGCAGAACATTTAACTAGCTGTGGTTTCAATTCTTGCCTCCGTG

Protein sequence

>MS.gene90176.t1

MDSAITVTTTAQMLLFRAFHRHFSGAPHRTLLRHNLKRPVTSYNNRSRIPCSSTSCTSTPLSPSLRNFRALLLRTPCRTRCVSSSAASFGSSAGGGNGGAGVGNGGGGGGSGSGGESGDGSVKLVGDAAQEVSALSPDVIILDVSGMVCGGCAASVKRILESQPQVSSASVNLTTETAIVWPVSEAKTAPNWQKQLGKTLAEHLTSCGFNSCLR