AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90606


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90606.t1 MTR_5g084570 96.000 50 2 0 1 50 1 50 1.37e-28 104
MS.gene90606.t1 MTR_5g084540 100.000 26 0 0 148 173 135 160 1.17e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 82 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTACATCTGATGCCAAAA+AGG 0.239988 5.4:-66869215 MS.gene90606:CDS
GTTGTAACCTCCACCATATT+TGG 0.288132 5.4:+66869038 None:intergenic
GAAGTAAACGATGCCAAATA+TGG 0.342429 5.4:-66869051 MS.gene90606:CDS
ACCTGCCATGGCAATGGTAT+TGG 0.432416 5.4:+66868740 None:intergenic
GAACCATAGCCAAGAGGCCT+AGG 0.443599 5.4:+66869278 None:intergenic
CATCCTAGGCCTCTTGGCTA+TGG 0.447886 5.4:-66869281 MS.gene90606:CDS
AATCCTCATCCTAGGCCTCT+TGG 0.458683 5.4:-66869287 MS.gene90606:CDS
GCCAATACCATTGCCATGGC+AGG 0.481585 5.4:-66868741 MS.gene90606:CDS
GGAGGTTTCAACCACGGTGG+TGG 0.498158 5.4:-66868991 MS.gene90606:CDS
AGTGTTGTCCTTAGCTTGCA+TGG 0.498460 5.4:+66868693 None:intergenic
GGTGGCTACAACCATGGTGG+AGG 0.500815 5.4:-66868790 MS.gene90606:CDS
GGTTTCAACCACGGTGGTGG+TGG 0.502796 5.4:-66868988 MS.gene90606:CDS
GGATACAACCACGGTGGTGG+TGG 0.504414 5.4:-66868949 MS.gene90606:CDS
GGTGGATACAACCACGGTGG+TGG 0.511490 5.4:-66868952 MS.gene90606:CDS
AGAAAAGCAATCCTCATCCT+AGG 0.516305 5.4:-66869295 MS.gene90606:CDS
GGATACAACAATGGTGGAGG+TGG 0.518813 5.4:-66868886 MS.gene90606:CDS
GGATACAACAATGGTGGAGG+TGG 0.518813 5.4:-66868928 MS.gene90606:CDS
GGAGGTTACAACCACGGCGG+TGG 0.523190 5.4:-66869027 MS.gene90606:CDS
GGCTACAACCATGGTGGAGG+CGG 0.523424 5.4:-66868787 MS.gene90606:CDS
GGCGGTTACAACCACGGTGG+AGG 0.523588 5.4:-66868769 MS.gene90606:CDS
GGATACAACCACGGTGGTGG+TGG 0.524452 5.4:-66868865 MS.gene90606:CDS
GGATACAACCACGGTGGTGG+TGG 0.524452 5.4:-66868907 MS.gene90606:CDS
TAGCCAAGAGGCCTAGGATG+AGG 0.538496 5.4:+66869284 None:intergenic
ATTTGTTGCCATGCAAGCTA+AGG 0.539995 5.4:-66868701 MS.gene90606:CDS
AATGGTGGTGGCTACAACCA+TGG 0.544536 5.4:-66868796 MS.gene90606:CDS
GGTTACAACCACGGCGGTGG+TGG 0.544864 5.4:-66869024 MS.gene90606:CDS
GGTGGTTACAACCATGGTGG+AGG 0.545718 5.4:-66868826 MS.gene90606:CDS
TTCCTCAGAAGTCTCAGCTA+GGG 0.554700 5.4:-66869248 MS.gene90606:CDS
GGTGGATACAACCACGGTGG+TGG 0.558361 5.4:-66868868 MS.gene90606:CDS
GGTGGATACAACCACGGTGG+TGG 0.558361 5.4:-66868910 MS.gene90606:CDS
CATGTGAGCAGCACCTGCCA+TGG 0.559419 5.4:+66868728 None:intergenic
TTTCCTCAGAAGTCTCAGCT+AGG 0.560253 5.4:-66869249 MS.gene90606:CDS
CATGGTGGAGGCGGTTACAA+TGG 0.561614 5.4:-66868814 MS.gene90606:CDS
GGATACAACAATGGTGGAGG+TGG 0.564967 5.4:-66868844 MS.gene90606:CDS
CACGGCGGTGGTGGTTACAA+TGG 0.571191 5.4:-66869015 MS.gene90606:CDS
GGTTACAACCACGGTGGAGG+CGG 0.572554 5.4:-66868766 MS.gene90606:CDS
AACGATGCCAAATATGGTGG+AGG 0.573315 5.4:-66869045 MS.gene90606:CDS
GGTGGAGGCGGTTACAATGG+TGG 0.575813 5.4:-66868811 MS.gene90606:CDS
GGTGGTGGTTACAATGGTGG+AGG 0.578963 5.4:-66869009 MS.gene90606:CDS
GGTTACAACCATGGTGGAGG+CGG 0.580077 5.4:-66868823 MS.gene90606:CDS
GGTGGATACAACAATGGCGG+TGG 0.583166 5.4:-66868970 MS.gene90606:CDS
GGCGGTGGTGGTTACAATGG+TGG 0.594919 5.4:-66869012 MS.gene90606:CDS
GGAGGCGGTTACAATGGTGG+TGG 0.595339 5.4:-66868808 MS.gene90606:CDS
GGTTGTAACCGCCTCCACCA+TGG 0.597627 5.4:+66868779 None:intergenic
GGTGGATACAACAATGGTGG+AGG 0.598383 5.4:-66868847 MS.gene90606:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.4:-66868889 MS.gene90606:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.4:-66868931 MS.gene90606:CDS
AGCAGCACCTGCCATGGCAA+TGG 0.620983 5.4:+66868734 None:intergenic
CATTGTAACCGCCTCCACCA+TGG 0.638839 5.4:+66868815 None:intergenic
GGTGGAGGCGGTTACAACCA+CGG 0.641005 5.4:-66868775 MS.gene90606:CDS
GGTTGCCAATACCATTGCCA+TGG 0.642022 5.4:-66868745 MS.gene90606:CDS
GTCCCTAGCTGAGACTTCTG+AGG 0.645915 5.4:+66869246 None:intergenic
CATTGTAACCACCACCGCCG+TGG 0.646466 5.4:+66869016 None:intergenic
GTAAACGATGCCAAATATGG+TGG 0.647144 5.4:-66869048 MS.gene90606:CDS
TAAGAAGAACCATAGCCAAG+AGG 0.664133 5.4:+66869272 None:intergenic
GGTGGTGGTGGATACAACAA+TGG 0.666044 5.4:-66868976 MS.gene90606:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.4:-66868850 MS.gene90606:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.4:-66868892 MS.gene90606:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.4:-66868934 MS.gene90606:CDS
ATTGGCAACCGCCTCCACCG+TGG 0.678280 5.4:+66868758 None:intergenic
GGTGGAGGTGGTTACAACCA+TGG 0.679817 5.4:-66868832 MS.gene90606:CDS
AATGGTGGAGGTTTCAACCA+CGG 0.680337 5.4:-66868997 MS.gene90606:CDS
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.4:-66868853 MS.gene90606:CDS
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.4:-66868895 MS.gene90606:CDS
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.4:-66868937 MS.gene90606:CDS
TATGGTGGAGGTTACAACCA+CGG 0.702708 5.4:-66869033 MS.gene90606:CDS
TGTTGTATCCACCACCACCG+TGG 0.714982 5.4:+66868857 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.4:+66868899 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.4:+66868941 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.4:+66868980 None:intergenic
GGTGGTGGCTACAACCATGG+TGG 0.723784 5.4:-66868793 MS.gene90606:CDS
GGTGGAGGTGGATACAACCA+CGG 0.728168 5.4:-66868874 MS.gene90606:CDS
GGTGGAGGTGGATACAACCA+CGG 0.728168 5.4:-66868916 MS.gene90606:CDS
AATGGCGGTGGATACAACCA+CGG 0.732761 5.4:-66868958 MS.gene90606:CDS
GGTGGTGGATACAACAATGG+CGG 0.734675 5.4:-66868973 MS.gene90606:CDS
GGAGGTGGTTACAACCATGG+TGG 0.740649 5.4:-66868829 MS.gene90606:CDS
GGCGGTGGATACAACCACGG+TGG 0.764343 5.4:-66868955 MS.gene90606:CDS
GGTGGAGGTTTCAACCACGG+TGG 0.772592 5.4:-66868994 MS.gene90606:CDS
GGAGGCGGTTACAACCACGG+TGG 0.788813 5.4:-66868772 MS.gene90606:CDS
GGAGGTGGATACAACCACGG+TGG 0.823069 5.4:-66868871 MS.gene90606:CDS
GGAGGTGGATACAACCACGG+TGG 0.823069 5.4:-66868913 MS.gene90606:CDS
GGTGGAGGTTACAACCACGG+CGG 0.825418 5.4:-66869030 MS.gene90606:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATATAGCTCACCCTTTT+TGG + chr5.4:66868790-66868809 None:intergenic 30.0%
AACTACATCTGATGCCAAAA+AGG - chr5.4:66868773-66868792 MS.gene90606:CDS 35.0%
ACTACATCTGATGCCAAAAA+GGG - chr5.4:66868774-66868793 MS.gene90606:CDS 35.0%
GAAGTAAACGATGCCAAATA+TGG - chr5.4:66868937-66868956 MS.gene90606:CDS 35.0%
AGAAAAGCAATCCTCATCCT+AGG - chr5.4:66868693-66868712 MS.gene90606:CDS 40.0%
GTAAACGATGCCAAATATGG+TGG - chr5.4:66868940-66868959 MS.gene90606:CDS 40.0%
GTTGTAACCTCCACCATATT+TGG + chr5.4:66868953-66868972 None:intergenic 40.0%
TAAGAAGAACCATAGCCAAG+AGG + chr5.4:66868719-66868738 None:intergenic 40.0%
! ATTTGTTGCCATGCAAGCTA+AGG - chr5.4:66869287-66869306 MS.gene90606:CDS 40.0%
AACGATGCCAAATATGGTGG+AGG - chr5.4:66868943-66868962 MS.gene90606:CDS 45.0%
TTCCTCAGAAGTCTCAGCTA+GGG - chr5.4:66868740-66868759 MS.gene90606:CDS 45.0%
TTTCCTCAGAAGTCTCAGCT+AGG - chr5.4:66868739-66868758 MS.gene90606:CDS 45.0%
! AATGGTGGAGGTTTCAACCA+CGG - chr5.4:66868991-66869010 MS.gene90606:CDS 45.0%
! TATGGTGGAGGTTACAACCA+CGG - chr5.4:66868955-66868974 MS.gene90606:CDS 45.0%
!! AGTGTTGTCCTTAGCTTGCA+TGG + chr5.4:66869298-66869317 None:intergenic 45.0%
AATCCTCATCCTAGGCCTCT+TGG - chr5.4:66868701-66868720 MS.gene90606:CDS 50.0%
AATGGCGGTGGATACAACCA+CGG - chr5.4:66869030-66869049 MS.gene90606:CDS 50.0%
ACCTGCCATGGCAATGGTAT+TGG + chr5.4:66869251-66869270 None:intergenic 50.0%
GGTGGATACAACAATGGTGG+AGG - chr5.4:66869057-66869076 MS.gene90606:CDS 50.0%
GGTTGCCAATACCATTGCCA+TGG - chr5.4:66869243-66869262 MS.gene90606:CDS 50.0%
! GGATACAACAATGGTGGAGG+TGG - chr5.4:66869060-66869079 MS.gene90606:CDS 50.0%
!! AATGGTGGTGGCTACAACCA+TGG - chr5.4:66869192-66869211 MS.gene90606:intron 50.0%
!! GGTGGTGGATACAACAATGG+CGG - chr5.4:66869015-66869034 MS.gene90606:CDS 50.0%
!! GGTGGTGGATACAACAATGG+TGG - chr5.4:66869015-66869034 MS.gene90606:CDS 50.0%
!! GGTGGTGGTGGATACAACAA+TGG - chr5.4:66869012-66869031 MS.gene90606:CDS 50.0%
CATCCTAGGCCTCTTGGCTA+TGG - chr5.4:66868707-66868726 MS.gene90606:CDS 55.0%
CATTGTAACCGCCTCCACCA+TGG + chr5.4:66869176-66869195 None:intergenic 55.0%
GAACCATAGCCAAGAGGCCT+AGG + chr5.4:66868713-66868732 None:intergenic 55.0%
GCCAATACCATTGCCATGGC+AGG - chr5.4:66869247-66869266 MS.gene90606:CDS 55.0%
GGAGGTGGTTACAACCATGG+TGG - chr5.4:66869159-66869178 MS.gene90606:intron 55.0%
GGTGGAGGTGGATACAACCA+CGG - chr5.4:66869072-66869091 MS.gene90606:CDS 55.0%
GGTGGAGGTGGTTACAACCA+TGG - chr5.4:66869156-66869175 MS.gene90606:intron 55.0%
GGTGGATACAACAATGGCGG+TGG - chr5.4:66869018-66869037 MS.gene90606:CDS 55.0%
GGTGGTTACAACCATGGTGG+AGG - chr5.4:66869162-66869181 MS.gene90606:intron 55.0%
GTCCCTAGCTGAGACTTCTG+AGG + chr5.4:66868745-66868764 None:intergenic 55.0%
TAGCCAAGAGGCCTAGGATG+AGG + chr5.4:66868707-66868726 None:intergenic 55.0%
TGTTGTATCCACCACCACCG+TGG + chr5.4:66869011-66869030 None:intergenic 55.0%
! CATGGTGGAGGCGGTTACAA+TGG - chr5.4:66869174-66869193 MS.gene90606:intron 55.0%
! GGTGGTGGTTACAATGGTGG+AGG - chr5.4:66868979-66868998 MS.gene90606:CDS 55.0%
! GGTTACAACCATGGTGGAGG+CGG - chr5.4:66869165-66869184 MS.gene90606:intron 55.0%
AGCAGCACCTGCCATGGCAA+TGG + chr5.4:66869257-66869276 None:intergenic 60.0%
CATGTGAGCAGCACCTGCCA+TGG + chr5.4:66869263-66869282 None:intergenic 60.0%
CATTGTAACCACCACCGCCG+TGG + chr5.4:66868975-66868994 None:intergenic 60.0%
GGAGGCGGTTACAATGGTGG+TGG - chr5.4:66869180-66869199 MS.gene90606:intron 60.0%
GGAGGTGGATACAACCACGG+TGG - chr5.4:66869075-66869094 MS.gene90606:intron 60.0%
GGAGGTTTCAACCACGGTGG+TGG - chr5.4:66868997-66869016 MS.gene90606:CDS 60.0%
GGTGGAGGCGGTTACAACCA+CGG - chr5.4:66869213-66869232 MS.gene90606:intron 60.0%
GGTGGAGGCGGTTACAATGG+TGG - chr5.4:66869177-66869196 MS.gene90606:intron 60.0%
GGTGGAGGTTACAACCACGG+CGG - chr5.4:66868958-66868977 MS.gene90606:CDS 60.0%
GGTGGAGGTTTCAACCACGG+TGG - chr5.4:66868994-66869013 MS.gene90606:CDS 60.0%
GGTGGATACAACCACGGTGG+TGG - chr5.4:66869036-66869055 MS.gene90606:CDS 60.0%
GGTGGCTACAACCATGGTGG+AGG - chr5.4:66869198-66869217 MS.gene90606:intron 60.0%
GGTTACAACCACGGTGGAGG+CGG - chr5.4:66869222-66869241 MS.gene90606:CDS 60.0%
GGTTGTAACCGCCTCCACCA+TGG + chr5.4:66869212-66869231 None:intergenic 60.0%
! CACGGCGGTGGTGGTTACAA+TGG - chr5.4:66868973-66868992 MS.gene90606:CDS 60.0%
! GGATACAACCACGGTGGTGG+TGG - chr5.4:66869039-66869058 MS.gene90606:CDS 60.0%
! GGCGGTGGTGGTTACAATGG+TGG - chr5.4:66868976-66868995 MS.gene90606:CDS 60.0%
! GGCTACAACCATGGTGGAGG+CGG - chr5.4:66869201-66869220 MS.gene90606:intron 60.0%
! GGTGGTGGCTACAACCATGG+TGG - chr5.4:66869195-66869214 MS.gene90606:intron 60.0%
! GGTTTCAACCACGGTGGTGG+TGG - chr5.4:66869000-66869019 MS.gene90606:CDS 60.0%
ATTGGCAACCGCCTCCACCG+TGG + chr5.4:66869233-66869252 None:intergenic 65.0%
GGAGGCGGTTACAACCACGG+TGG - chr5.4:66869216-66869235 MS.gene90606:CDS 65.0%
GGAGGTTACAACCACGGCGG+TGG - chr5.4:66868961-66868980 MS.gene90606:CDS 65.0%
GGCGGTGGATACAACCACGG+TGG - chr5.4:66869033-66869052 MS.gene90606:CDS 65.0%
GGCGGTTACAACCACGGTGG+AGG - chr5.4:66869219-66869238 MS.gene90606:CDS 65.0%
GGTTACAACCACGGCGGTGG+TGG - chr5.4:66868964-66868983 MS.gene90606:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 66868684 66869326 66868684 ID=MS.gene90606
chr5.4 mRNA 66868684 66869326 66868684 ID=MS.gene90606.t1;Parent=MS.gene90606
chr5.4 exon 66869215 66869326 66869215 ID=MS.gene90606.t1.exon1;Parent=MS.gene90606.t1
chr5.4 CDS 66869215 66869326 66869215 ID=cds.MS.gene90606.t1;Parent=MS.gene90606.t1
chr5.4 exon 66868684 66869093 66868684 ID=MS.gene90606.t1.exon2;Parent=MS.gene90606.t1
chr5.4 CDS 66868684 66869093 66868684 ID=cds.MS.gene90606.t1;Parent=MS.gene90606.t1
Gene Sequence

>MS.gene90606

ATGGATTCGAGAAAAGCAATCCTCATCCTAGGCCTCTTGGCTATGGTTCTTCTTATTTCCTCAGAAGTCTCAGCTAGGGACTTAACTGAAACTACATCTGATGCCAAAAAGGGTGAGCTATATATTATTTTTCTTTATAAATACTTTCACGAGCATTTTCTATATTGTTTAAATTCTTTTCTAGTTAATTATATATAAAGTAATGATTCATATTATATGTGCTGTGTTTGCAGAAGTTGTTGAAAAGACTAATGAAGTAAACGATGCCAAATATGGTGGAGGTTACAACCACGGCGGTGGTGGTTACAATGGTGGAGGTTTCAACCACGGTGGTGGTGGATACAACAATGGCGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGTTACAACCATGGTGGAGGCGGTTACAATGGTGGTGGCTACAACCATGGTGGAGGCGGTTACAACCACGGTGGAGGCGGTTGCCAATACCATTGCCATGGCAGGTGCTGCTCACATGCTGAATTTGTTGCCATGCAAGCTAAGGACAACACTCAGAACTGA

Protein sequence

>MS.gene90606.t1

MDSRKAILILGLLAMVLLISSEVSARDLTETTSDAKKEVVEKTNEVNDAKYGGGYNHGGGGYNGGGFNHGGGGYNNGGGYNHGGGGYNNGGGGYNHGGGGYNNGGGGYNHGGGGYNNGGGGYNHGGGGYNGGGYNHGGGGYNHGGGGCQYHCHGRCCSHAEFVAMQAKDNTQN