AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91451

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91451.t1 MTR_7g108340 91.753 97 7 1 47 142 570 666 5.13e-52 177
MS.gene91451.t1 MTR_2g437390 77.660 94 20 1 50 142 580 673 1.90e-43 154
MS.gene91451.t1 MTR_4g119413 78.723 94 19 1 50 142 582 675 2.43e-43 153
MS.gene91451.t1 MTR_2g019260 30.833 120 67 2 16 124 588 702 4.09e-11 61.2
MS.gene91451.t1 MTR_2g019260 30.833 120 67 2 16 124 588 702 4.56e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91451.t1 AT4G02570 78.351 97 20 1 47 142 573 669 6.63e-45 158
MS.gene91451.t1 AT4G02570 78.351 97 20 1 47 142 573 669 6.63e-45 158
MS.gene91451.t1 AT4G02570 78.351 97 20 1 47 142 573 669 6.63e-45 158
MS.gene91451.t1 AT4G02570 78.351 97 20 1 47 142 573 669 6.63e-45 158
MS.gene91451.t1 AT1G43140 71.134 97 27 1 47 142 556 652 1.81e-39 142
MS.gene91451.t1 AT1G02980 69.149 94 28 1 50 142 580 673 1.61e-37 137
MS.gene91451.t1 AT1G02980 69.149 94 28 1 50 142 580 673 1.61e-37 137
MS.gene91451.t1 AT5G46210 31.667 120 66 2 16 124 588 702 1.81e-12 65.5

Find 44 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAACTCAAAGCTGTTATT+TGG 0.120691 4.2:-66495887 None:intergenic
CTTCCGCTCAATCGCGCTTT+TGG 0.258241 4.2:+66495483 MS.gene91451:CDS
GGGGATATTGTTCTAGTGTT+AGG 0.298977 4.2:-66495866 None:intergenic
TGAACTCAAAGCTGTTATTT+GGG 0.303552 4.2:-66495886 None:intergenic
CAATCTTACTTTAGGTTCCT+TGG 0.325619 4.2:+66497499 MS.gene91451:intron
TGATAAAATGAGAAGGATAA+AGG 0.328384 4.2:+66495922 MS.gene91451:CDS
GATGGAAAATTAGAGGAAAA+AGG 0.337103 4.2:+66497608 MS.gene91451:CDS
TCATTTGCAGAGCAGCAAAT+TGG 0.384248 4.2:+66495402 MS.gene91451:CDS
CCTTAGCTTTCTTCCCCAAC+TGG 0.415006 4.2:-66497537 None:intergenic
ATGTTCAAGGTCAACCAGTT+GGG 0.422615 4.2:+66497523 MS.gene91451:CDS
TTGAAGATGTTGACAAAGAT+AGG 0.427442 4.2:+66496080 MS.gene91451:CDS
ATTTGAAGATTCTCTTCTAC+AGG 0.429642 4.2:+66495703 MS.gene91451:intron
TAGGTTCCTTGGAATGTTCA+AGG 0.430171 4.2:+66497510 MS.gene91451:intron
CAATTAGGATGGAAAATTAG+AGG 0.433231 4.2:+66497601 MS.gene91451:CDS
TTCGTCGGGAGGTTCCAATT+AGG 0.436289 4.2:+66497586 MS.gene91451:CDS
ATAACCTTCCTTTCATCGAC+AGG 0.464796 4.2:-66496058 None:intergenic
AAAATATATGATGCTTGATA+AGG 0.466748 4.2:+66495841 MS.gene91451:CDS
CATTGGAGTCATCATTCGTC+GGG 0.486293 4.2:+66497572 MS.gene91451:CDS
TTGACCGTTGAACCGATTCA+AGG 0.487199 4.2:-66495442 None:intergenic
CCGCAGTGCTGAATAGTAGC+AGG 0.487589 4.2:-66495733 None:intergenic
CTTTCATCGACAGGCGGAAG+AGG 0.488133 4.2:-66496049 None:intergenic
CAAAAGCGCGATTGAGCGGA+AGG 0.515414 4.2:-66495482 None:intergenic
GAGAATGAAGCAATCTAACT+AGG 0.520531 4.2:-66495808 None:intergenic
CTCTTCCGCCTGTCGATGAA+AGG 0.522925 4.2:+66496050 MS.gene91451:CDS
AGCGCGATTGAGCGGAAGGG+TGG 0.540009 4.2:-66495478 None:intergenic
TAAAGTAACTTACAAAACCT+TGG 0.544348 4.2:-66497631 None:intergenic
TCGGGAGGTTCCAATTAGGA+TGG 0.555563 4.2:+66497590 MS.gene91451:CDS
TCACCAAAAGCGCGATTGAG+CGG 0.556194 4.2:-66495486 None:intergenic
AATGTTCAAGGTCAACCAGT+TGG 0.558156 4.2:+66497522 MS.gene91451:CDS
GAACTCAAAGCTGTTATTTG+GGG 0.565800 4.2:-66495885 None:intergenic
TTTCATCGACAGGCGGAAGA+GGG 0.573315 4.2:-66496048 None:intergenic
ACATTGGAGTCATCATTCGT+CGG 0.575208 4.2:+66497571 MS.gene91451:CDS
GGTTGACCTTGAACATTCCA+AGG 0.575296 4.2:-66497516 None:intergenic
ATCACCTTGAATCGGTTCAA+CGG 0.575542 4.2:+66495438 MS.gene91451:CDS
GCGCGATTGAGCGGAAGGGT+GGG 0.585850 4.2:-66495477 None:intergenic
TGTTCAAGGTCAACCAGTTG+GGG 0.587428 4.2:+66497524 MS.gene91451:CDS
CCAGTTGGGGAAGAAAGCTA+AGG 0.591633 4.2:+66497537 MS.gene91451:CDS
TCCGCCTGTCGATGAAAGGA+AGG 0.598612 4.2:+66496054 MS.gene91451:CDS
TGGAGTCATCATTCGTCGGG+AGG 0.606488 4.2:+66497575 MS.gene91451:CDS
CGCAGTGCTGAATAGTAGCA+GGG 0.616738 4.2:-66495732 None:intergenic
ACCTTCCTTTCATCGACAGG+CGG 0.628723 4.2:-66496055 None:intergenic
AAAAGCGCGATTGAGCGGAA+GGG 0.653250 4.2:-66495481 None:intergenic
AATTTACTGATAAAATGAGA+AGG 0.656477 4.2:+66495915 MS.gene91451:CDS
CCTGCTACTATTCAGCACTG+CGG 0.795381 4.2:+66495733 MS.gene91451:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAACAAAATACAACTAA+TGG + chr4.2:66495553-66495572 MS.gene91451:intron 15.0%
!! AAAATATATGATGCTTGATA+AGG + chr4.2:66495841-66495860 MS.gene91451:CDS 20.0%
!! AATCTTATAAAAAGTGACTA+AGG - chr4.2:66496372-66496391 None:intergenic 20.0%
!! AATTTACTGATAAAATGAGA+AGG + chr4.2:66495915-66495934 MS.gene91451:CDS 20.0%
!! CATGCAAAACATAATTAATT+TGG - chr4.2:66496512-66496531 None:intergenic 20.0%
!! TAAAATTTATACAGCAACTA+AGG - chr4.2:66496272-66496291 None:intergenic 20.0%
!! TATCATTTCTCTTAATATTG+AGG + chr4.2:66497255-66497274 MS.gene91451:intron 20.0%
!!! AGTTTCAGATTTTTATTAAC+AGG - chr4.2:66496013-66496032 None:intergenic 20.0%
!!! ATTGATTTGATAGATGTTAA+AGG - chr4.2:66496594-66496613 None:intergenic 20.0%
!!! GTATTTTGTTAATTAATGAC+CGG - chr4.2:66495547-66495566 None:intergenic 20.0%
!!! GTTTCAGATTTTTATTAACA+GGG - chr4.2:66496012-66496031 None:intergenic 20.0%
!!! TCTCATTTTATCAGTAAATT+TGG - chr4.2:66495915-66495934 None:intergenic 20.0%
!!! TTGATTTGATAGATGTTAAA+GGG - chr4.2:66496593-66496612 None:intergenic 20.0%
!!! TTGTTAATGTTAATGAGTTT+TGG + chr4.2:66496235-66496254 MS.gene91451:intron 20.0%
!!! TTTGGTGAATGTATTTTTAA+AGG + chr4.2:66495501-66495520 MS.gene91451:CDS 20.0%
! AAAAAAAGATCACCTTGAAT+CGG + chr4.2:66495430-66495449 MS.gene91451:CDS 25.0%
! AAAATATGTTAAACGAGAGT+AGG - chr4.2:66496949-66496968 None:intergenic 25.0%
! AATCGAATGTTTACTTTGAT+AGG + chr4.2:66496485-66496504 MS.gene91451:intron 25.0%
! TAAAGTAACTTACAAAACCT+TGG - chr4.2:66497634-66497653 None:intergenic 25.0%
! TACTTCTATACATCTACAAA+TGG + chr4.2:66496544-66496563 MS.gene91451:intron 25.0%
! TGATAAAATGAGAAGGATAA+AGG + chr4.2:66495922-66495941 MS.gene91451:CDS 25.0%
!! CATGAATTTGAAGTTATTGT+TGG + chr4.2:66497342-66497361 MS.gene91451:intron 25.0%
!! TCTCGTTTAACATATTTTAC+AGG + chr4.2:66496951-66496970 MS.gene91451:intron 25.0%
!! TTATGTCCTTGCATTTTATA+TGG + chr4.2:66497371-66497390 MS.gene91451:intron 25.0%
!!! CTAAGTTCTAAATCTTATGA+GGG - chr4.2:66495965-66495984 None:intergenic 25.0%
!!! TCACAAAAGTTGTTGATTTT+TGG - chr4.2:66497103-66497122 None:intergenic 25.0%
!!! TCTACTTTTCCTTTGAAAAT+TGG - chr4.2:66497132-66497151 None:intergenic 25.0%
!!! TTTTGCGATCTATATTTTTG+TGG + chr4.2:66497301-66497320 MS.gene91451:intron 25.0%
AAAGCCTACCATAAAATCAA+TGG - chr4.2:66496344-66496363 None:intergenic 30.0%
ACAGAACCATATAAAATGCA+AGG - chr4.2:66497380-66497399 None:intergenic 30.0%
ACTCCAAAACTAATTGTTGA+TGG - chr4.2:66496162-66496181 None:intergenic 30.0%
ATACCATGCAATCTTACTTT+AGG + chr4.2:66497491-66497510 MS.gene91451:intron 30.0%
ATCAAATCAATAGCAAGCTT+AGG + chr4.2:66496603-66496622 MS.gene91451:intron 30.0%
ATTTGAAGATTCTCTTCTAC+AGG + chr4.2:66495703-66495722 MS.gene91451:intron 30.0%
CAATAAACCCATAAAAACAG+CGG - chr4.2:66496889-66496908 None:intergenic 30.0%
CAATTAGGATGGAAAATTAG+AGG + chr4.2:66497601-66497620 MS.gene91451:CDS 30.0%
GAGACAATATGTAAGATCAA+AGG - chr4.2:66496712-66496731 None:intergenic 30.0%
GATGGAAAATTAGAGGAAAA+AGG + chr4.2:66497608-66497627 MS.gene91451:CDS 30.0%
TCCTGACTAACATGTTAAAA+TGG + chr4.2:66496832-66496851 MS.gene91451:intron 30.0%
TGAGTAAATAGAAAACCATG+AGG - chr4.2:66495591-66495610 None:intergenic 30.0%
TGTTATCATCATCTTCACTT+GGG + chr4.2:66495613-66495632 MS.gene91451:intron 30.0%
TTGAACTCAAAGCTGTTATT+TGG - chr4.2:66495890-66495909 None:intergenic 30.0%
TTGAAGATGTTGACAAAGAT+AGG + chr4.2:66496080-66496099 MS.gene91451:CDS 30.0%
TTGTTATCATCATCTTCACT+TGG + chr4.2:66495612-66495631 MS.gene91451:intron 30.0%
! ATGAAGAGTAGGAAAGTTTT+AGG + chr4.2:66496136-66496155 MS.gene91451:intron 30.0%
! CTCTAATTTTCCATCCTAAT+TGG - chr4.2:66497603-66497622 None:intergenic 30.0%
! TAGATGTATAGAAGTATGAG+AGG - chr4.2:66496540-66496559 None:intergenic 30.0%
! TGAACTCAAAGCTGTTATTT+GGG - chr4.2:66495889-66495908 None:intergenic 30.0%
!! AATGTTAATGAGTTTTGGTG+CGG + chr4.2:66496240-66496259 MS.gene91451:intron 30.0%
!! ACTTTTCCTTTGAAAATTGG+TGG - chr4.2:66497129-66497148 None:intergenic 30.0%
!! GCTAAGTTCTAAATCTTATG+AGG - chr4.2:66495966-66495985 None:intergenic 30.0%
!! TAAGGTTTTGTCAGTTACAT+TGG + chr4.2:66497555-66497574 MS.gene91451:CDS 30.0%
!! TCCATTTTAACATGTTAGTC+AGG - chr4.2:66496836-66496855 None:intergenic 30.0%
!! TGCATTTTATATGGTTCTGT+TGG + chr4.2:66497380-66497399 MS.gene91451:intron 30.0%
!!! CTTTGTAATTTTTTCGCATG+CGG - chr4.2:66497013-66497032 None:intergenic 30.0%
!!! TTGTGTTTTGATATCTACGA+AGG + chr4.2:66496801-66496820 MS.gene91451:intron 30.0%
!!! TTTGTAATTTTTTCGCATGC+GGG - chr4.2:66497012-66497031 None:intergenic 30.0%
AAATTAGAGGAAAAAGGCCA+AGG + chr4.2:66497614-66497633 MS.gene91451:CDS 35.0%
AAGTCCAAAAATCTCCACAA+AGG - chr4.2:66497444-66497463 None:intergenic 35.0%
ACTTCAACTAATGGTAAACG+TGG + chr4.2:66496980-66496999 MS.gene91451:intron 35.0%
ACTTTCTCTCTCTATTGCTT+TGG - chr4.2:66497169-66497188 None:intergenic 35.0%
AGAAGCCATGGAGTTAATAT+TGG + chr4.2:66497058-66497077 MS.gene91451:intron 35.0%
AGGAAAAGTAGATAGTGACA+CGG + chr4.2:66497140-66497159 MS.gene91451:intron 35.0%
AGGTGAATGACTTCAACTAA+TGG + chr4.2:66496971-66496990 MS.gene91451:intron 35.0%
CCTACCATAAAATCAATGGA+AGG - chr4.2:66496340-66496359 None:intergenic 35.0%
GAACCTAAAGTAAGATTGCA+TGG - chr4.2:66497497-66497516 None:intergenic 35.0%
GAGAATGAAGCAATCTAACT+AGG - chr4.2:66495811-66495830 None:intergenic 35.0%
GAGAGATAAAGTTGTCACAT+AGG + chr4.2:66497209-66497228 MS.gene91451:intron 35.0%
TCAAAACACAAGCCATGATA+AGG - chr4.2:66496792-66496811 None:intergenic 35.0%
TCACATAGGTTGTCACAAAA+TGG + chr4.2:66497223-66497242 MS.gene91451:intron 35.0%
TTACTATGTCATGTCCTTTG+TGG + chr4.2:66497427-66497446 MS.gene91451:intron 35.0%
TTCTAAGCTTTGCCTTATCA+TGG + chr4.2:66496777-66496796 MS.gene91451:intron 35.0%
TTGCTCCAATATTAACTCCA+TGG - chr4.2:66497066-66497085 None:intergenic 35.0%
! ATGTCCTTTGTGGAGATTTT+TGG + chr4.2:66497437-66497456 MS.gene91451:intron 35.0%
! CAATCTTACTTTAGGTTCCT+TGG + chr4.2:66497499-66497518 MS.gene91451:intron 35.0%
! GAACTCAAAGCTGTTATTTG+GGG - chr4.2:66495888-66495907 None:intergenic 35.0%
! TGACAACCACCAATTTTCAA+AGG + chr4.2:66497120-66497139 MS.gene91451:intron 35.0%
!! AGGCCATCAACAATTAGTTT+TGG + chr4.2:66496156-66496175 MS.gene91451:intron 35.0%
!!! CCTTCCATTGATTTTATGGT+AGG + chr4.2:66496337-66496356 MS.gene91451:intron 35.0%
!!! GCTTCCTTCCATTGATTTTA+TGG + chr4.2:66496333-66496352 MS.gene91451:intron 35.0%
!!! TTGTAATTTTTTCGCATGCG+GGG - chr4.2:66497011-66497030 None:intergenic 35.0%
AAGAGAAGAGGATGAGTCTT+AGG - chr4.2:66495653-66495672 None:intergenic 40.0%
AATGTTCAAGGTCAACCAGT+TGG + chr4.2:66497522-66497541 MS.gene91451:CDS 40.0%
ACAAAGCAACGATGAAGTTC+AGG + chr4.2:66496666-66496685 MS.gene91451:intron 40.0%
ACATTGGAGTCATCATTCGT+CGG + chr4.2:66497571-66497590 MS.gene91451:CDS 40.0%
ATAACCTTCCTTTCATCGAC+AGG - chr4.2:66496061-66496080 None:intergenic 40.0%
ATACGCTCTTGATTCTCTTC+CGG + chr4.2:66495525-66495544 MS.gene91451:intron 40.0%
ATGACCACCAAGAGTTTCAA+TGG + chr4.2:66496638-66496657 MS.gene91451:intron 40.0%
ATGTTCAAGGTCAACCAGTT+GGG + chr4.2:66497523-66497542 MS.gene91451:CDS 40.0%
ATTCACAAAGCTAGAAGCCA+TGG + chr4.2:66497046-66497065 MS.gene91451:intron 40.0%
GAAGGTGAAATGAGTAGTTG+CGG - chr4.2:66496753-66496772 None:intergenic 40.0%
GGCAAAGCTTAGAAAGAAGA+AGG - chr4.2:66496771-66496790 None:intergenic 40.0%
GGGAAAAAGAATTGAGAACC+TGG - chr4.2:66495992-66496011 None:intergenic 40.0%
GGGGATATTGTTCTAGTGTT+AGG - chr4.2:66495869-66495888 None:intergenic 40.0%
TAGGTTCCTTGGAATGTTCA+AGG + chr4.2:66497510-66497529 MS.gene91451:intron 40.0%
TCAGCATAACAGAAGAGAAG+AGG - chr4.2:66495665-66495684 None:intergenic 40.0%
TCATTTGCAGAGCAGCAAAT+TGG + chr4.2:66495402-66495421 MS.gene91451:CDS 40.0%
TGGTTATGTTGTGTTCCTCA+TGG + chr4.2:66495573-66495592 MS.gene91451:intron 40.0%
TTGTGTGCATCATGAAGAGT+AGG + chr4.2:66496125-66496144 MS.gene91451:intron 40.0%
! AACTCTTGGTGGTCATCAAA+AGG - chr4.2:66496634-66496653 None:intergenic 40.0%
! ATCACCTTGAATCGGTTCAA+CGG + chr4.2:66495438-66495457 MS.gene91451:CDS 40.0%
! GATTTAGAACTTAGCTAGCC+AGG + chr4.2:66495971-66495990 MS.gene91451:intron 40.0%
! GTTTGTGCCATTGAAACTCT+TGG - chr4.2:66496648-66496667 None:intergenic 40.0%
!! AAGCCTTCACTTTAGCTTGA+GGG - chr4.2:66496317-66496336 None:intergenic 40.0%
AAATGAGTAGTTGCGGTGGA+AGG - chr4.2:66496746-66496765 None:intergenic 45.0%
AATGAGTAGTTGCGGTGGAA+GGG - chr4.2:66496745-66496764 None:intergenic 45.0%
CAAAGGTTCCTCGTTTACGA+TGG - chr4.2:66496695-66496714 None:intergenic 45.0%
CATTGGAGTCATCATTCGTC+GGG + chr4.2:66497572-66497591 MS.gene91451:CDS 45.0%
CTACCCTCAAGCTAAAGTGA+AGG + chr4.2:66496311-66496330 MS.gene91451:intron 45.0%
GAAGAGGATGAGTCTTAGGA+AGG - chr4.2:66495649-66495668 None:intergenic 45.0%
GGTTGACCTTGAACATTCCA+AGG - chr4.2:66497519-66497538 None:intergenic 45.0%
TGCCAGGCATCTAAACACTA+CGG - chr4.2:66496865-66496884 None:intergenic 45.0%
TGTTCAAGGTCAACCAGTTG+GGG + chr4.2:66497524-66497543 MS.gene91451:CDS 45.0%
TTCCGTAGTGTTTAGATGCC+TGG + chr4.2:66496860-66496879 MS.gene91451:intron 45.0%
TTGGAGTGCGTTGAGCAATT+GGG + chr4.2:66496175-66496194 MS.gene91451:intron 45.0%
! TGTGCCATTGAAACTCTTGG+TGG - chr4.2:66496645-66496664 None:intergenic 45.0%
! TTGACCGTTGAACCGATTCA+AGG - chr4.2:66495445-66495464 None:intergenic 45.0%
! TTTGGAGTGCGTTGAGCAAT+TGG + chr4.2:66496174-66496193 MS.gene91451:intron 45.0%
!! CTTCACTTTAGCTTGAGGGT+AGG - chr4.2:66496313-66496332 None:intergenic 45.0%
!! GAAGCCTTCACTTTAGCTTG+AGG - chr4.2:66496318-66496337 None:intergenic 45.0%
ACCTTCCTTTCATCGACAGG+CGG - chr4.2:66496058-66496077 None:intergenic 50.0%
CCAGTTGGGGAAGAAAGCTA+AGG + chr4.2:66497537-66497556 MS.gene91451:CDS 50.0%
CCTGCTACTATTCAGCACTG+CGG + chr4.2:66495733-66495752 MS.gene91451:CDS 50.0%
GGTGAAATGAGTAGTTGCGG+TGG - chr4.2:66496750-66496769 None:intergenic 50.0%
TCAGGTGTCCATCGTAAACG+AGG + chr4.2:66496684-66496703 MS.gene91451:intron 50.0%
TCGGGAGGTTCCAATTAGGA+TGG + chr4.2:66497590-66497609 MS.gene91451:CDS 50.0%
TGGAGTGCGTTGAGCAATTG+GGG + chr4.2:66496176-66496195 MS.gene91451:intron 50.0%
TTCGTCGGGAGGTTCCAATT+AGG + chr4.2:66497586-66497605 MS.gene91451:CDS 50.0%
TTTCATCGACAGGCGGAAGA+GGG - chr4.2:66496051-66496070 None:intergenic 50.0%
! AAAAGCGCGATTGAGCGGAA+GGG - chr4.2:66495484-66495503 None:intergenic 50.0%
! CCTTAGCTTTCTTCCCCAAC+TGG - chr4.2:66497540-66497559 None:intergenic 50.0%
! CGCAGTGCTGAATAGTAGCA+GGG - chr4.2:66495735-66495754 None:intergenic 50.0%
! TCACCAAAAGCGCGATTGAG+CGG - chr4.2:66495489-66495508 None:intergenic 50.0%
!!! CTGGCAGCCGCTGTTTTTAT+GGG + chr4.2:66496879-66496898 MS.gene91451:intron 50.0%
ACAGGCGGAAGAGGGATCTA+TGG - chr4.2:66496043-66496062 None:intergenic 55.0%
AGTAGTTGCGGTGGAAGGGA+AGG - chr4.2:66496741-66496760 None:intergenic 55.0%
CCATAAAAACAGCGGCTGCC+AGG - chr4.2:66496881-66496900 None:intergenic 55.0%
CTCTTCCGCCTGTCGATGAA+AGG + chr4.2:66496050-66496069 MS.gene91451:CDS 55.0%
CTTTCATCGACAGGCGGAAG+AGG - chr4.2:66496052-66496071 None:intergenic 55.0%
GCAATTGGGGCGCATGTTCA+AGG + chr4.2:66496189-66496208 MS.gene91451:intron 55.0%
TCCGCCTGTCGATGAAAGGA+AGG + chr4.2:66496054-66496073 MS.gene91451:CDS 55.0%
TGGAGTCATCATTCGTCGGG+AGG + chr4.2:66497575-66497594 MS.gene91451:CDS 55.0%
! CAAAAGCGCGATTGAGCGGA+AGG - chr4.2:66495485-66495504 None:intergenic 55.0%
! CCGCAGTGCTGAATAGTAGC+AGG - chr4.2:66495736-66495755 None:intergenic 55.0%
! CTTCCGCTCAATCGCGCTTT+TGG + chr4.2:66495483-66495502 MS.gene91451:CDS 55.0%
!!! CCTGGCAGCCGCTGTTTTTA+TGG + chr4.2:66496878-66496897 MS.gene91451:intron 55.0%
AGCGCGATTGAGCGGAAGGG+TGG - chr4.2:66495481-66495500 None:intergenic 65.0%
GCGCGATTGAGCGGAAGGGT+GGG - chr4.2:66495480-66495499 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 66495387 66497654 66495387 ID=MS.gene91451
chr4.2 mRNA 66495387 66497654 66495387 ID=MS.gene91451.t1;Parent=MS.gene91451
chr4.2 exon 66495387 66495522 66495387 ID=MS.gene91451.t1.exon1;Parent=MS.gene91451.t1
chr4.2 CDS 66495387 66495522 66495387 ID=cds.MS.gene91451.t1;Parent=MS.gene91451.t1
chr4.2 exon 66495711 66495943 66495711 ID=MS.gene91451.t1.exon2;Parent=MS.gene91451.t1
chr4.2 CDS 66495711 66495943 66495711 ID=cds.MS.gene91451.t1;Parent=MS.gene91451.t1
chr4.2 exon 66496046 66496101 66496046 ID=MS.gene91451.t1.exon3;Parent=MS.gene91451.t1
chr4.2 CDS 66496046 66496101 66496046 ID=cds.MS.gene91451.t1;Parent=MS.gene91451.t1
chr4.2 exon 66497513 66497654 66497513 ID=MS.gene91451.t1.exon4;Parent=MS.gene91451.t1
chr4.2 CDS 66497513 66497654 66497513 ID=cds.MS.gene91451.t1;Parent=MS.gene91451.t1
Gene Sequence

>MS.gene91451

ATGTGTATTGTTTCATCATTTGCAGAGCAGCAAATTGGTCACAAAAAAAAGATCACCTTGAATCGGTTCAACGGTCAATGTTCGACTTATCCCACCCTTCCGCTCAATCGCGCTTTTGGTGAATGTATTTTTAAAGGTATACGCTCTTGATTCTCTTCCGGTCATTAATTAACAAAATACAACTAATGGTTATGTTGTGTTCCTCATGGTTTTCTATTTACTCACTTGTTATCATCATCTTCACTTGGGAAAGAAATTACCTTCCTAAGACTCATCCTCTTCTCTTCTGTTATGCTGATTATTGTGACACTGAAATATTTGAAGATTCTCTTCTACAGGCTGCTGCCCTGCTACTATTCAGCACTGCGGATAAGCTTAGTTATTCAGAGATTATGACTCAGCTGAACTTGACTAATGAAGACCTAGTTAGATTGCTTCATTCTCTATCTTGCGCAAAATATATGATGCTTGATAAGGAGCCTAACACTAGAACAATATCCCCAAATAACAGCTTTGAGTTCAATTCCAAATTTACTGATAAAATGAGAAGGATAAAGGTTTGAATTTACTTTCAACCCTCATAAGATTTAGAACTTAGCTAGCCAGGTTCTCAATTCTTTTTCCCTGTTAATAAAAATCTGAAACTTTGTTGACCATAGATCCCTCTTCCGCCTGTCGATGAAAGGAAGGTTATTGAAGATGTTGACAAAGATAGGTGATATGCTATTGATGCAGCAATTGTGTGCATCATGAAGAGTAGGAAAGTTTTAGGCCATCAACAATTAGTTTTGGAGTGCGTTGAGCAATTGGGGCGCATGTTCAAGGTAAAATTTGCTTGAGTTAATTTATTGTTAATGTTAATGAGTTTTGGTGCGGAAAGTACCTTAGTTGCTGTATAAATTTTATCACAACATCATAGTCTACCTACCCTCAAGCTAAAGTGAAGGCTTCCTTCCATTGATTTTATGGTAGGCTTTTACTACCTTAGTCACTTTTTATAAGATTTTTTTTTATGAAAACAAACTCAATTAATTTCATTAATAATTTTTTTTTGTTAAATGAGTATTTTGATGCTACTTTTATGTTGTTTATGTATGAAATCGAATGTTTACTTTGATAGGACCAAATTAATTATGTTTTGCATGAGTAACCTCTCATACTTCTATACATCTACAAATGGCTCTTTACGTGTGAAATTGTTTGCCCTTTAACATCTATCAAATCAATAGCAAGCTTAGGAGTAGCCTTTTGATGACCACCAAGAGTTTCAATGGCACAAACAAAGCAACGATGAAGTTCAGGTGTCCATCGTAAACGAGGAACCTTTGATCTTACATATTGTCTCGCTACACCTTCCCTTCCACCGCAACTACTCATTTCACCTTCTTCTTTCTAAGCTTTGCCTTATCATGGCTTGTGTTTTGATATCTACGAAGGACACATTGTCCTGACTAACATGTTAAAATGGATTCATTCCGTAGTGTTTAGATGCCTGGCAGCCGCTGTTTTTATGGGTTTATTGTTATTGATTCTCATTTTGAATGTTAATTATTCTTTATCCTACTCTCGTTTAACATATTTTACAGGTGAATGACTTCAACTAATGGTAAACGTGGCGTGACCCCGCATGCGAAAAAATTACAAAGCTGCATACAAAAAATTCACAAAGCTAGAAGCCATGGAGTTAATATTGGAGCAATGATATTCTAACAACCAAAAATCAACAACTTTTGTGACAACCACCAATTTTCAAAGGAAAAGTAGATAGTGACACGGAAACCAAAGCAATAGAGAGAGAAAGTAAAAAAAATAATGTGAGTATGAGAGATAAAGTTGTCACATAGGTTGTCACAAAATGGTTGTTAGAATATCATTTCTCTTAATATTGAGGTAACTACATGCTTTATTAACAGTTTTTGCGATCTATATTTTTGTGGTTCTATATTTTTGTTTAACATGAATTTGAAGTTATTGTTGGACATACTTATGTCCTTGCATTTTATATGGTTCTGTTGGTTTTTGTGTTCAAGTGATGTTTGTTTACTATGTCATGTCCTTTGTGGAGATTTTTGGACTTGTTAATGCATTGCTGAATTCATTTTGTATACCATGCAATCTTACTTTAGGTTCCTTGGAATGTTCAAGGTCAACCAGTTGGGGAAGAAAGCTAAGGTTTTGTCAGTTACATTGGAGTCATCATTCGTCGGGAGGTTCCAATTAGGATGGAAAATTAGAGGAAAAAGGCCAAGGTTTTGTAAGTTACTTTAG
Protein sequence

>MS.gene91451.t1

MCIVSSFAEQQIGHKKKITLNRFNGQCSTYPTLPLNRAFGECIFKDSLLQAAALLLFSTADKLSYSEIMTQLNLTNEDLVRLLHSLSCAKYMMLDKEPNTRTISPNNSFEFNSKFTDKMRRIKIPLPPVDERKVIEDVDKDRFLGMFKVNQLGKKAKVLSVTLESSFVGRFQLGWKIRGKRPRFCKLL