Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92196.t1 | XP_013464259.1 | 84.6 | 188 | 17 | 3 | 1 | 186 | 152 | 329 | 9.00E-76 | 293.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92196.t1 | A0A072V8C9 | 84.6 | 188 | 17 | 3 | 1 | 186 | 152 | 329 | 6.5e-76 | 293.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene92196 | 0.841606 | 4.29E-58 | -1.69E-46 |
| MS.gene049254 | MS.gene92196 | 0.805992 | 1.02E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene92196 | 0.834196 | 3.45E-56 | -1.69E-46 |
| MS.gene049647 | MS.gene92196 | 0.807259 | 5.52E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene92196 | 0.801509 | 8.71E-49 | -1.69E-46 |
| MS.gene050657 | MS.gene92196 | 0.813187 | 2.91E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene92196 | 0.818545 | 1.85E-52 | -1.69E-46 |
| MS.gene051332 | MS.gene92196 | 0.807601 | 4.67E-50 | -1.69E-46 |
| MS.gene051388 | MS.gene92196 | 0.820072 | 8.32E-53 | -1.69E-46 |
| MS.gene051490 | MS.gene92196 | 0.833422 | 5.38E-56 | -1.69E-46 |
| MS.gene051744 | MS.gene92196 | 0.803952 | 2.73E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene92196 | 0.821168 | 4.66E-53 | -1.69E-46 |
| MS.gene053164 | MS.gene92196 | 0.820801 | 5.66E-53 | -1.69E-46 |
| MS.gene054467 | MS.gene92196 | 0.805303 | 1.43E-49 | -1.69E-46 |
| MS.gene054576 | MS.gene92196 | 0.828185 | 1.03E-54 | -1.69E-46 |
| MS.gene05458 | MS.gene92196 | 0.812754 | 3.62E-51 | -1.69E-46 |
| MS.gene05468 | MS.gene92196 | 0.806199 | 9.24E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene92196 | 0.813251 | 2.81E-51 | -1.69E-46 |
| MS.gene054991 | MS.gene92196 | 0.843439 | 1.40E-58 | -1.69E-46 |
| MS.gene055926 | MS.gene92196 | 0.815567 | 8.65E-52 | -1.69E-46 |
| MS.gene056050 | MS.gene92196 | 0.810382 | 1.19E-50 | -1.69E-46 |
| MS.gene056142 | MS.gene92196 | 0.803802 | 2.93E-49 | -1.69E-46 |
| MS.gene056595 | MS.gene92196 | 0.803154 | 3.99E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene92196 | 0.804954 | 1.69E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene92196 | 0.802815 | 4.69E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene92196 | 0.859582 | 3.74E-63 | -1.69E-46 |
| MS.gene059570 | MS.gene92196 | 0.824141 | 9.48E-54 | -1.69E-46 |
| MS.gene059774 | MS.gene92196 | 0.806786 | 6.95E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene92196 | 0.807185 | 5.72E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene92196 | 0.804871 | 1.75E-49 | -1.69E-46 |
| MS.gene060697 | MS.gene92196 | 0.805822 | 1.11E-49 | -1.69E-46 |
| MS.gene060777 | MS.gene92196 | 0.818368 | 2.03E-52 | -1.69E-46 |
| MS.gene060785 | MS.gene92196 | 0.80328 | 3.76E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene92196 | 0.808307 | 3.30E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92196.t1 | MTR_2g067420 | 92.553 | 188 | 2 | 3 | 1 | 186 | 152 | 329 | 1.55e-113 | 326 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGATTCTGAATTAGATTT+TGG | 0.138125 | 2.4:-27199311 | MS.gene92196:CDS |
| AATTATTGTTGTTAACAATT+TGG | 0.189180 | 2.4:+27199678 | None:intergenic |
| GAACAAGAATTGGCTTCTTT+AGG | 0.224414 | 2.4:-27199653 | MS.gene92196:CDS |
| AACAAGAATTGGCTTCTTTA+GGG | 0.269704 | 2.4:-27199652 | MS.gene92196:CDS |
| GGGAATCAGATTATGATCTT+TGG | 0.282083 | 2.4:-27199562 | MS.gene92196:CDS |
| GAATTTGATGATGACCCTTT+TGG | 0.284214 | 2.4:+27199740 | None:intergenic |
| GATGAATTTGAGAATGAGTT+TGG | 0.300902 | 2.4:-27199449 | MS.gene92196:CDS |
| TGGTTTATAAATTAAACTTT+TGG | 0.304291 | 2.4:+27199248 | None:intergenic |
| TGTTCAACTTCATAATTCTT+TGG | 0.308448 | 2.4:+27199380 | None:intergenic |
| TGGCCCTCTTTGCCTCAAAA+TGG | 0.309901 | 2.4:-27199275 | MS.gene92196:CDS |
| GGTATTTCAATCAAATTGTT+TGG | 0.324518 | 2.4:+27199587 | None:intergenic |
| CAATAATTTAGAACAAGAAT+TGG | 0.355039 | 2.4:-27199663 | MS.gene92196:CDS |
| GAGAATGAGTTTGGAGTAGT+AGG | 0.357141 | 2.4:-27199440 | MS.gene92196:CDS |
| CCAAAATCTAATTCAGAATC+AGG | 0.359166 | 2.4:+27199311 | None:intergenic |
| GATTATAACAAGTTCATTGT+TGG | 0.377497 | 2.4:+27199772 | None:intergenic |
| ATAATCAATGAGGATCCAAA+AGG | 0.471989 | 2.4:-27199755 | MS.gene92196:CDS |
| CAACTTCATAATTCTTTGGT+AGG | 0.492672 | 2.4:+27199384 | None:intergenic |
| TGGAGTAGTAGGTGAAACAA+AGG | 0.494712 | 2.4:-27199429 | MS.gene92196:CDS |
| GGAACTTATCAATCAAATGT+TGG | 0.506519 | 2.4:-27199521 | MS.gene92196:CDS |
| ACAATTTGATTGAAATACCA+TGG | 0.523468 | 2.4:-27199583 | MS.gene92196:CDS |
| TTGAAATCAAATGCCTCATG+AGG | 0.570871 | 2.4:+27199347 | None:intergenic |
| TGGCAATATATTAGAAGATG+TGG | 0.578763 | 2.4:-27199615 | MS.gene92196:CDS |
| CAATTTGATTGAAATACCAT+GGG | 0.579155 | 2.4:-27199582 | MS.gene92196:CDS |
| TAATCAATGAGGATCCAAAA+GGG | 0.599310 | 2.4:-27199754 | MS.gene92196:CDS |
| AGATCATAATCTGATTCCCA+TGG | 0.633397 | 2.4:+27199566 | None:intergenic |
| GGAGTCAAACAACAAAGATG+AGG | 0.641607 | 2.4:-27199408 | MS.gene92196:CDS |
| TGAAATCAAATGCCTCATGA+GGG | 0.643825 | 2.4:+27199348 | None:intergenic |
| TGAACTTGTTATAATCAATG+AGG | 0.647171 | 2.4:-27199765 | MS.gene92196:CDS |
| ACAAGAAATTGACCCTCATG+AGG | 0.656432 | 2.4:-27199360 | MS.gene92196:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTATTGTTGTTAACAATT+TGG | + | chr2.4:27199381-27199400 | None:intergenic | 15.0% |
| !!! | ATTTTGGTAATATTCAATTG+TGG | - | chr2.4:27199761-27199780 | MS.gene92196:CDS | 20.0% |
| !!! | CAATAATTTAGAACAAGAAT+TGG | - | chr2.4:27199393-27199412 | MS.gene92196:CDS | 20.0% |
| !!! | TGGAATTTTATTGACAATAT+TGG | - | chr2.4:27199514-27199533 | MS.gene92196:CDS | 20.0% |
| ! | ACAATTTGATTGAAATACCA+TGG | - | chr2.4:27199473-27199492 | MS.gene92196:CDS | 25.0% |
| ! | ATGTTGTTGAAGCAAAAAAT+TGG | - | chr2.4:27199575-27199594 | MS.gene92196:CDS | 25.0% |
| ! | CAATTTGATTGAAATACCAT+GGG | - | chr2.4:27199474-27199493 | MS.gene92196:CDS | 25.0% |
| ! | GATTATAACAAGTTCATTGT+TGG | + | chr2.4:27199287-27199306 | None:intergenic | 25.0% |
| ! | GGTATTTCAATCAAATTGTT+TGG | + | chr2.4:27199472-27199491 | None:intergenic | 25.0% |
| ! | TGTTCAACTTCATAATTCTT+TGG | + | chr2.4:27199679-27199698 | None:intergenic | 25.0% |
| !!! | TGAACTTGTTATAATCAATG+AGG | - | chr2.4:27199291-27199310 | MS.gene92196:CDS | 25.0% |
| ATAATCAATGAGGATCCAAA+AGG | - | chr2.4:27199301-27199320 | MS.gene92196:CDS | 30.0% | |
| CAACTTCATAATTCTTTGGT+AGG | + | chr2.4:27199675-27199694 | None:intergenic | 30.0% | |
| CCAAAATCTAATTCAGAATC+AGG | + | chr2.4:27199748-27199767 | None:intergenic | 30.0% | |
| GATGAATTTGAGAATGAGTT+TGG | - | chr2.4:27199607-27199626 | MS.gene92196:CDS | 30.0% | |
| GGAACTTATCAATCAAATGT+TGG | - | chr2.4:27199535-27199554 | MS.gene92196:CDS | 30.0% | |
| TAATCAATGAGGATCCAAAA+GGG | - | chr2.4:27199302-27199321 | MS.gene92196:CDS | 30.0% | |
| TGGCAATATATTAGAAGATG+TGG | - | chr2.4:27199441-27199460 | MS.gene92196:CDS | 30.0% | |
| ! | AACAAGAATTGGCTTCTTTA+GGG | - | chr2.4:27199404-27199423 | MS.gene92196:CDS | 30.0% |
| ! | CCTGATTCTGAATTAGATTT+TGG | - | chr2.4:27199745-27199764 | MS.gene92196:CDS | 30.0% |
| !! | TTAGGGTTTTATGATGATGT+TGG | - | chr2.4:27199421-27199440 | MS.gene92196:CDS | 30.0% |
| !!! | ACTTTTGGAATACCATTTTG+AGG | + | chr2.4:27199796-27199815 | None:intergenic | 30.0% |
| AGATCATAATCTGATTCCCA+TGG | + | chr2.4:27199493-27199512 | None:intergenic | 35.0% | |
| GGGAATCAGATTATGATCTT+TGG | - | chr2.4:27199494-27199513 | MS.gene92196:CDS | 35.0% | |
| TGAAATCAAATGCCTCATGA+GGG | + | chr2.4:27199711-27199730 | None:intergenic | 35.0% | |
| TTGAAATCAAATGCCTCATG+AGG | + | chr2.4:27199712-27199731 | None:intergenic | 35.0% | |
| ! | GAACAAGAATTGGCTTCTTT+AGG | - | chr2.4:27199403-27199422 | MS.gene92196:CDS | 35.0% |
| !!! | AATACCATTTTGAGGCAAAG+AGG | + | chr2.4:27199788-27199807 | None:intergenic | 35.0% |
| !!! | ATACCATTTTGAGGCAAAGA+GGG | + | chr2.4:27199787-27199806 | None:intergenic | 35.0% |
| !!! | GAATTTGATGATGACCCTTT+TGG | + | chr2.4:27199319-27199338 | None:intergenic | 35.0% |
| ACAAGAAATTGACCCTCATG+AGG | - | chr2.4:27199696-27199715 | MS.gene92196:CDS | 40.0% | |
| GGAGTCAAACAACAAAGATG+AGG | - | chr2.4:27199648-27199667 | MS.gene92196:CDS | 40.0% | |
| TGGAGTAGTAGGTGAAACAA+AGG | - | chr2.4:27199627-27199646 | MS.gene92196:CDS | 40.0% | |
| ! | GAGAATGAGTTTGGAGTAGT+AGG | - | chr2.4:27199616-27199635 | MS.gene92196:CDS | 40.0% |
| TGGCCCTCTTTGCCTCAAAA+TGG | - | chr2.4:27199781-27199800 | MS.gene92196:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 27199259 | 27199819 | 27199259 | ID=MS.gene92196 |
| chr2.4 | mRNA | 27199259 | 27199819 | 27199259 | ID=MS.gene92196.t1;Parent=MS.gene92196 |
| chr2.4 | exon | 27199259 | 27199819 | 27199259 | ID=MS.gene92196.t1.exon1;Parent=MS.gene92196.t1 |
| chr2.4 | CDS | 27199259 | 27199819 | 27199259 | ID=cds.MS.gene92196.t1;Parent=MS.gene92196.t1 |
| Gene Sequence |
| Protein sequence |
