AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93632


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93632.t1 MTR_6g065460 92.195 205 15 1 1 205 1 204 2.41e-128 360
MS.gene93632.t1 MTR_6g065460 82.439 205 11 1 1 205 1 180 4.84e-108 308
MS.gene93632.t1 MTR_6g059650 65.128 195 58 6 19 210 20 207 2.24e-79 236
MS.gene93632.t1 MTR_3g463590 56.284 183 71 4 27 205 26 203 1.85e-62 193
MS.gene93632.t1 MTR_6g059530 52.404 208 85 6 1 206 1 196 2.98e-54 172
MS.gene93632.t1 MTR_6g059760 50.237 211 92 9 1 206 1 203 2.25e-52 168
MS.gene93632.t1 MTR_6g059410 52.632 209 82 8 1 206 1 195 1.26e-51 166
MS.gene93632.t1 MTR_6g059730 50.739 203 94 5 6 206 7 205 4.87e-50 162
MS.gene93632.t1 MTR_6g045097 43.128 211 109 7 1 205 1 206 1.19e-45 151
MS.gene93632.t1 MTR_6g044780 42.925 212 110 7 1 206 1 207 1.35e-45 151
MS.gene93632.t1 MTR_6g044810 43.333 210 108 7 1 204 1 205 1.59e-45 150
MS.gene93632.t1 MTR_6g017225 47.596 208 95 7 6 205 7 208 4.48e-45 154
MS.gene93632.t1 MTR_6g045403 42.584 209 111 5 1 205 1 204 7.95e-45 149
MS.gene93632.t1 MTR_6g059680 49.495 198 90 6 15 206 16 209 1.75e-44 148
MS.gene93632.t1 MTR_6g045433 40.758 211 102 6 7 203 8 209 1.70e-39 135
MS.gene93632.t1 MTR_6g066190 42.453 212 107 8 1 206 1 203 6.26e-38 131
MS.gene93632.t1 MTR_6g066210 42.180 211 111 6 1 206 1 205 6.58e-38 131
MS.gene93632.t1 MTR_6g059810 43.062 209 101 10 4 202 8 208 2.83e-37 129
MS.gene93632.t1 MTR_6g009650 41.706 211 110 7 1 206 1 203 9.50e-37 127
MS.gene93632.t1 MTR_6g009563 39.906 213 112 8 1 206 1 204 9.65e-33 117
MS.gene93632.t1 MTR_1g035770 37.383 214 123 7 6 211 7 217 4.27e-31 114
MS.gene93632.t1 MTR_6g065570 77.778 63 14 0 14 76 11 73 2.31e-29 105
MS.gene93632.t1 MTR_6g059755 50.877 114 49 5 96 206 11 120 5.45e-28 103
MS.gene93632.t1 MTR_1g057110 36.279 215 100 11 1 206 1 187 2.62e-27 102
MS.gene93632.t1 MTR_6g078100 37.209 215 115 11 1 205 1 205 6.82e-25 97.1
MS.gene93632.t1 MTR_6g078070 35.814 215 116 11 1 205 1 203 1.05e-24 96.7
MS.gene93632.t1 MTR_6g078290 34.722 216 120 9 1 202 1 209 1.06e-23 94.4
MS.gene93632.t1 MTR_6g078140 32.000 200 114 8 21 205 21 213 1.65e-17 77.8
MS.gene93632.t1 MTR_6g078120 32.000 200 114 8 21 205 21 213 4.24e-17 76.6
MS.gene93632.t1 MTR_3g106585 33.491 212 111 12 5 206 7 198 1.51e-15 72.4
MS.gene93632.t1 MTR_3g106620 30.108 186 111 8 28 206 27 200 3.11e-15 71.6
MS.gene93632.t1 MTR_2g073010 28.713 202 128 7 7 205 10 198 2.43e-14 68.9
MS.gene93632.t1 MTR_0302s0030 28.856 201 134 6 7 202 3 199 1.05e-13 67.4
MS.gene93632.t1 MTR_3g014870 28.293 205 127 8 5 202 9 200 7.20e-13 65.1
MS.gene93632.t1 MTR_6g078280 29.293 198 122 9 21 205 20 212 2.80e-12 63.5
MS.gene93632.t1 MTR_3g014820 27.805 205 128 8 5 202 9 200 4.51e-12 63.2
MS.gene93632.t1 MTR_2g072980 28.643 199 133 7 7 205 10 199 6.24e-12 62.4
MS.gene93632.t1 MTR_8g059790 26.601 203 137 8 6 202 5 201 8.97e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93632.t1 AT1G17860 33.824 204 122 8 4 205 3 195 1.86e-21 88.2
MS.gene93632.t1 AT1G73260 31.429 210 131 7 1 207 8 207 1.10e-20 86.3
MS.gene93632.t1 AT1G73325 31.884 207 126 9 4 205 7 203 6.85e-18 79.3

Find 59 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATCATGCCATCAATCTT+TGG 0.296622 6.2:+70707884 MS.gene93632:CDS
CCTAGCCTGCTAAACTTTAC+AGG 0.305071 6.2:-70708001 None:intergenic
GGGAGGCATGATGACTTTAC+TGG 0.309229 6.2:+70708289 MS.gene93632:CDS
TGTTGATGCCTCTCTCAAAA+TGG 0.313339 6.2:+70708357 MS.gene93632:CDS
CCTGGTGCTTGTTATGATAT+TGG 0.331841 6.2:+70708268 MS.gene93632:CDS
TGACTTTACTGGAAGGCTTC+TGG 0.336007 6.2:+70708300 MS.gene93632:CDS
CTGGTGCTTGTTATGATATT+GGG 0.341631 6.2:+70708269 MS.gene93632:CDS
GGTAAGGAATGTAGGTTTCA+TGG 0.342364 6.2:-70707750 None:intergenic
TACTGGAAGGCTTCTGGTCC+TGG 0.347355 6.2:+70708306 MS.gene93632:CDS
GAAGGTAAGCATATTATAGA+TGG 0.366110 6.2:+70708175 MS.gene93632:CDS
ATCAACAAATACAACTTCAT+AGG 0.388203 6.2:-70708341 None:intergenic
CTTGGAGGATTCAGCACACT+TGG 0.412692 6.2:-70708083 None:intergenic
CCAGGTGGGGCAGTGATAGA+TGG 0.414356 6.2:-70708250 None:intergenic
CCAGCTGCTCCAAAGATTGA+TGG 0.422912 6.2:-70707893 None:intergenic
ATCTTTGGAGCAGCTGGTGG+TGG 0.441234 6.2:+70707899 MS.gene93632:CDS
GAACAAGTTATTGACACAAA+TGG 0.443270 6.2:+70707833 MS.gene93632:CDS
CCATCTATCACTGCCCCACC+TGG 0.453523 6.2:+70708250 MS.gene93632:CDS
AAAGAAAGGGTAAGGAATGT+AGG 0.459147 6.2:-70707758 None:intergenic
CCATCAATCTTTGGAGCAGC+TGG 0.460514 6.2:+70707893 MS.gene93632:CDS
ACTGGACCATGGGTGGGTAT+TGG 0.466256 6.2:+70708136 MS.gene93632:CDS
GAGCAGCTGGTGGTGGATTG+AGG 0.467057 6.2:+70707906 MS.gene93632:CDS
GTCTGCCTGTAAAGTTTAGC+AGG 0.472799 6.2:+70707996 MS.gene93632:CDS
GGACCATGGGTGGGTATTGG+TGG 0.483131 6.2:+70708139 MS.gene93632:CDS
TGGACAGAACACAAACTTGT+AGG 0.486171 6.2:-70708230 None:intergenic
CCTGTAAAGTTTAGCAGGCT+AGG 0.493193 6.2:+70708001 MS.gene93632:CDS
GATTATTCCGAGGTTGTCAA+TGG 0.494338 6.2:+70707974 MS.gene93632:CDS
CATAGGGATCATTATTAGCC+AGG 0.494704 6.2:-70708324 None:intergenic
TAACAAATTAACTGGACCAT+GGG 0.499496 6.2:+70708126 MS.gene93632:CDS
AGAGAAGCAAAGAAAGGGTA+AGG 0.502150 6.2:-70707766 None:intergenic
CAAATTAACTGGACCATGGG+TGG 0.513850 6.2:+70708129 MS.gene93632:CDS
ATGCAAAGAGAAGCAAAGAA+AGG 0.517116 6.2:-70707772 None:intergenic
TGCAAAGAGAAGCAAAGAAA+GGG 0.522946 6.2:-70707771 None:intergenic
TGCAAAAGCTAATGAAAAGC+AGG 0.523206 6.2:-70707801 None:intergenic
ATCATCAACCAACACCCACT+TGG 0.538112 6.2:-70708101 None:intergenic
TCAACAAATACAACTTCATA+GGG 0.541440 6.2:-70708340 None:intergenic
AAGTTTAGCAGGCTAGGTGC+AGG 0.543812 6.2:+70708007 MS.gene93632:CDS
ATCAACCAACACCCACTTGG+AGG 0.563736 6.2:-70708098 None:intergenic
TGAATCCTCCAAGTGGGTGT+TGG 0.564748 6.2:+70708093 MS.gene93632:CDS
TTGGAGGATTCAGCACACTT+GGG 0.577442 6.2:-70708082 None:intergenic
TTAACAAATTAACTGGACCA+TGG 0.578865 6.2:+70708125 MS.gene93632:CDS
ATGATATAGAATCTGCCACC+AGG 0.579132 6.2:-70707869 None:intergenic
GGTGGTACTGAAGATAATGA+AGG 0.579997 6.2:+70708157 MS.gene93632:CDS
ATCATAACAAGCACCAGGTG+GGG 0.587156 6.2:-70708263 None:intergenic
CCAATATCATAACAAGCACC+AGG 0.593116 6.2:-70708268 None:intergenic
TCAATCTTTGGAGCAGCTGG+TGG 0.597041 6.2:+70707896 MS.gene93632:CDS
TAATCTTGAAGTACAGTCAG+TGG 0.598215 6.2:-70707956 None:intergenic
TATCATAACAAGCACCAGGT+GGG 0.615337 6.2:-70708264 None:intergenic
GCTGGTGGTGGATTGAGGCT+TGG 0.621479 6.2:+70707911 MS.gene93632:CDS
GTTGTAAATGCGATATCAAG+TGG 0.626836 6.2:-70708055 None:intergenic
AGGCAGACCATTGACAACCT+CGG 0.627216 6.2:-70707981 None:intergenic
AAATTAACTGGACCATGGGT+GGG 0.632486 6.2:+70708130 MS.gene93632:CDS
GTGCTTGTTATGATATTGGG+AGG 0.648866 6.2:+70708272 MS.gene93632:CDS
GTACCACCAATACCCACCCA+TGG 0.653889 6.2:-70708142 None:intergenic
GGCATGATGACTTTACTGGA+AGG 0.654511 6.2:+70708293 MS.gene93632:CDS
TGTACTTCAAGATTATTCCG+AGG 0.664559 6.2:+70707964 MS.gene93632:CDS
GTGTGCTGAATCCTCCAAGT+GGG 0.697546 6.2:+70708087 MS.gene93632:CDS
ATATCATAACAAGCACCAGG+TGG 0.697776 6.2:-70708265 None:intergenic
AAAAGCTAATGAAAAGCAGG+TGG 0.699196 6.2:-70707798 None:intergenic
AGTGTGCTGAATCCTCCAAG+TGG 0.756355 6.2:+70708086 MS.gene93632:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTAAAATTCAAAAACA+TGG + chr6.2:70708199-70708218 MS.gene93632:CDS 15.0%
!!! GATGATTTTAACAAATTAAC+TGG + chr6.2:70708118-70708137 MS.gene93632:CDS 20.0%
! ATCAACAAATACAACTTCAT+AGG - chr6.2:70708344-70708363 None:intergenic 25.0%
! TCAACAAATACAACTTCATA+GGG - chr6.2:70708343-70708362 None:intergenic 25.0%
GAACAAGTTATTGACACAAA+TGG + chr6.2:70707833-70707852 MS.gene93632:CDS 30.0%
GAAGGTAAGCATATTATAGA+TGG + chr6.2:70708175-70708194 MS.gene93632:CDS 30.0%
TAACAAATTAACTGGACCAT+GGG + chr6.2:70708126-70708145 MS.gene93632:CDS 30.0%
TATATCATGCCATCAATCTT+TGG + chr6.2:70707884-70707903 MS.gene93632:CDS 30.0%
TTAACAAATTAACTGGACCA+TGG + chr6.2:70708125-70708144 MS.gene93632:CDS 30.0%
AAAAGCTAATGAAAAGCAGG+TGG - chr6.2:70707801-70707820 None:intergenic 35.0%
AAAGAAAGGGTAAGGAATGT+AGG - chr6.2:70707761-70707780 None:intergenic 35.0%
ATGCAAAGAGAAGCAAAGAA+AGG - chr6.2:70707775-70707794 None:intergenic 35.0%
GTTGTAAATGCGATATCAAG+TGG - chr6.2:70708058-70708077 None:intergenic 35.0%
TAATCTTGAAGTACAGTCAG+TGG - chr6.2:70707959-70707978 None:intergenic 35.0%
TGCAAAAGCTAATGAAAAGC+AGG - chr6.2:70707804-70707823 None:intergenic 35.0%
TGCAAAGAGAAGCAAAGAAA+GGG - chr6.2:70707774-70707793 None:intergenic 35.0%
TGTACTTCAAGATTATTCCG+AGG + chr6.2:70707964-70707983 MS.gene93632:CDS 35.0%
!! AAGATCTTCCATTTTGAGAG+AGG - chr6.2:70708368-70708387 None:intergenic 35.0%
!! AATGGAAACCCTATTTTTCC+TGG + chr6.2:70707851-70707870 MS.gene93632:CDS 35.0%
!! CTGGTGCTTGTTATGATATT+GGG + chr6.2:70708269-70708288 MS.gene93632:CDS 35.0%
AAATTAACTGGACCATGGGT+GGG + chr6.2:70708130-70708149 MS.gene93632:CDS 40.0%
AATCTGCCACCAGGAAAAAT+AGG - chr6.2:70707863-70707882 None:intergenic 40.0%
AGAGAAGCAAAGAAAGGGTA+AGG - chr6.2:70707769-70707788 None:intergenic 40.0%
ATATCATAACAAGCACCAGG+TGG - chr6.2:70708268-70708287 None:intergenic 40.0%
ATCTGCCACCAGGAAAAATA+GGG - chr6.2:70707862-70707881 None:intergenic 40.0%
ATGATATAGAATCTGCCACC+AGG - chr6.2:70707872-70707891 None:intergenic 40.0%
CATAGGGATCATTATTAGCC+AGG - chr6.2:70708327-70708346 None:intergenic 40.0%
CCAATATCATAACAAGCACC+AGG - chr6.2:70708271-70708290 None:intergenic 40.0%
GATTATTCCGAGGTTGTCAA+TGG + chr6.2:70707974-70707993 MS.gene93632:CDS 40.0%
TATCATAACAAGCACCAGGT+GGG - chr6.2:70708267-70708286 None:intergenic 40.0%
TGGACAGAACACAAACTTGT+AGG - chr6.2:70708233-70708252 None:intergenic 40.0%
TGTTGATGCCTCTCTCAAAA+TGG + chr6.2:70708357-70708376 MS.gene93632:CDS 40.0%
! GGTGGTACTGAAGATAATGA+AGG + chr6.2:70708157-70708176 MS.gene93632:CDS 40.0%
!! CCTGGTGCTTGTTATGATAT+TGG + chr6.2:70708268-70708287 MS.gene93632:CDS 40.0%
!! GTGCTTGTTATGATATTGGG+AGG + chr6.2:70708272-70708291 MS.gene93632:CDS 40.0%
ATCATAACAAGCACCAGGTG+GGG - chr6.2:70708266-70708285 None:intergenic 45.0%
ATCATCAACCAACACCCACT+TGG - chr6.2:70708104-70708123 None:intergenic 45.0%
CAAATTAACTGGACCATGGG+TGG + chr6.2:70708129-70708148 MS.gene93632:CDS 45.0%
CCTAGCCTGCTAAACTTTAC+AGG - chr6.2:70708004-70708023 None:intergenic 45.0%
CCTGTAAAGTTTAGCAGGCT+AGG + chr6.2:70708001-70708020 MS.gene93632:CDS 45.0%
GGCATGATGACTTTACTGGA+AGG + chr6.2:70708293-70708312 MS.gene93632:CDS 45.0%
GTCTGCCTGTAAAGTTTAGC+AGG + chr6.2:70707996-70708015 MS.gene93632:CDS 45.0%
TGACTTTACTGGAAGGCTTC+TGG + chr6.2:70708300-70708319 MS.gene93632:CDS 45.0%
TTGGAGGATTCAGCACACTT+GGG - chr6.2:70708085-70708104 None:intergenic 45.0%
!! GGAAACCCTATTTTTCCTGG+TGG + chr6.2:70707854-70707873 MS.gene93632:CDS 45.0%
AAGTTTAGCAGGCTAGGTGC+AGG + chr6.2:70708007-70708026 MS.gene93632:CDS 50.0%
AGGCAGACCATTGACAACCT+CGG - chr6.2:70707984-70708003 None:intergenic 50.0%
AGTGTGCTGAATCCTCCAAG+TGG + chr6.2:70708086-70708105 MS.gene93632:CDS 50.0%
ATCAACCAACACCCACTTGG+AGG - chr6.2:70708101-70708120 None:intergenic 50.0%
CCAGCTGCTCCAAAGATTGA+TGG - chr6.2:70707896-70707915 None:intergenic 50.0%
CTTGGAGGATTCAGCACACT+TGG - chr6.2:70708086-70708105 None:intergenic 50.0%
GTGTGCTGAATCCTCCAAGT+GGG + chr6.2:70708087-70708106 MS.gene93632:CDS 50.0%
TGAATCCTCCAAGTGGGTGT+TGG + chr6.2:70708093-70708112 MS.gene93632:CDS 50.0%
! GGGAGGCATGATGACTTTAC+TGG + chr6.2:70708289-70708308 MS.gene93632:CDS 50.0%
!! CCATCAATCTTTGGAGCAGC+TGG + chr6.2:70707893-70707912 MS.gene93632:CDS 50.0%
!! TCAATCTTTGGAGCAGCTGG+TGG + chr6.2:70707896-70707915 MS.gene93632:CDS 50.0%
ACTGGACCATGGGTGGGTAT+TGG + chr6.2:70708136-70708155 MS.gene93632:CDS 55.0%
GTACCACCAATACCCACCCA+TGG - chr6.2:70708145-70708164 None:intergenic 55.0%
TACTGGAAGGCTTCTGGTCC+TGG + chr6.2:70708306-70708325 MS.gene93632:CDS 55.0%
!! ATCTTTGGAGCAGCTGGTGG+TGG + chr6.2:70707899-70707918 MS.gene93632:CDS 55.0%
CCAGGTGGGGCAGTGATAGA+TGG - chr6.2:70708253-70708272 None:intergenic 60.0%
CCATCTATCACTGCCCCACC+TGG + chr6.2:70708250-70708269 MS.gene93632:CDS 60.0%
!! GAGCAGCTGGTGGTGGATTG+AGG + chr6.2:70707906-70707925 MS.gene93632:CDS 60.0%
!! GCTGGTGGTGGATTGAGGCT+TGG + chr6.2:70707911-70707930 MS.gene93632:CDS 60.0%
!! GGACCATGGGTGGGTATTGG+TGG + chr6.2:70708139-70708158 MS.gene93632:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 70707752 70708390 70707752 ID=MS.gene93632
chr6.2 mRNA 70707752 70708390 70707752 ID=MS.gene93632.t1;Parent=MS.gene93632
chr6.2 exon 70707752 70708390 70707752 ID=MS.gene93632.t1.exon1;Parent=MS.gene93632.t1
chr6.2 CDS 70707752 70708390 70707752 ID=cds.MS.gene93632.t1;Parent=MS.gene93632.t1
Gene Sequence

>MS.gene93632

ATGAAACCTACATTCCTTACCCTTTCTTTGCTTCTCTTTGCATTAACCACCTGCTTTTCATTAGCTTTTGCACAAGCTTCTGAACAAGTTATTGACACAAATGGAAACCCTATTTTTCCTGGTGGCAGATTCTATATCATGCCATCAATCTTTGGAGCAGCTGGTGGTGGATTGAGGCTTGGTAAGACAAAAAACTCAAAATGTCCACTGACTGTACTTCAAGATTATTCCGAGGTTGTCAATGGTCTGCCTGTAAAGTTTAGCAGGCTAGGTGCAGGAATTGATATAATCTCTACAAACACACCACTTGATATCGCATTTACAACAAAGCCCAAGTGTGCTGAATCCTCCAAGTGGGTGTTGGTTGATGATTTTAACAAATTAACTGGACCATGGGTGGGTATTGGTGGTACTGAAGATAATGAAGGTAAGCATATTATAGATGGTTTCTTTAAAATTCAAAAACATGGTTTAACTACCTACAAGTTTGTGTTCTGTCCATCTATCACTGCCCCACCTGGTGCTTGTTATGATATTGGGAGGCATGATGACTTTACTGGAAGGCTTCTGGTCCTGGCTAATAATGATCCCTATGAAGTTGTATTTGTTGATGCCTCTCTCAAAATGGAAGATCTTTAG

Protein sequence

>MS.gene93632.t1

MKPTFLTLSLLLFALTTCFSLAFAQASEQVIDTNGNPIFPGGRFYIMPSIFGAAGGGLRLGKTKNSKCPLTVLQDYSEVVNGLPVKFSRLGAGIDIISTNTPLDIAFTTKPKCAESSKWVLVDDFNKLTGPWVGIGGTEDNEGKHIIDGFFKIQKHGLTTYKFVFCPSITAPPGACYDIGRHDDFTGRLLVLANNDPYEVVFVDASLKMEDL