AlfalfaGEDB | MS.gene93638

M. sativa cultivar XinJiangDaYe / MS.gene93638

KEGG: K02894
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00238.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93638.t1	XP_018810074.1	95	101	5	0	1	101	1	101	8.80E-47	196.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93638.t1	Q07760	91.1	101	9	0	1	101	1	101	4.0e-48	191.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93638.t1	A0A2I4DSF1	95.0	101	5	0	1	101	1	101	6.3e-47	196.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene93638	0.855727	5.20E-62	-1.69E-46
MS.gene049404	MS.gene93638	0.806302	8.79E-50	-1.69E-46
MS.gene049435	MS.gene93638	0.806191	9.27E-50	-1.69E-46
MS.gene050020	MS.gene93638	0.816205	6.24E-52	-1.69E-46
MS.gene050442	MS.gene93638	0.803438	3.49E-49	-1.69E-46
MS.gene050807	MS.gene93638	0.801665	8.09E-49	-1.69E-46
MS.gene051198	MS.gene93638	0.820541	6.50E-53	-1.69E-46
MS.gene052020	MS.gene93638	0.802643	5.09E-49	-1.69E-46
MS.gene052863	MS.gene93638	0.805825	1.11E-49	-1.69E-46
MS.gene05289	MS.gene93638	0.813614	2.34E-51	-1.69E-46
MS.gene054236	MS.gene93638	0.849948	2.33E-60	-1.69E-46
MS.gene054775	MS.gene93638	0.801185	1.01E-48	-1.69E-46
MS.gene055608	MS.gene93638	0.828408	9.14E-55	-1.69E-46
MS.gene055818	MS.gene93638	0.821195	4.59E-53	-1.69E-46
MS.gene055926	MS.gene93638	0.801993	6.93E-49	-1.69E-46
MS.gene056173	MS.gene93638	0.806455	8.16E-50	-1.69E-46
MS.gene056174	MS.gene93638	0.802921	4.46E-49	-1.69E-46
MS.gene057444	MS.gene93638	0.808404	3.15E-50	-1.69E-46
MS.gene057693	MS.gene93638	0.802475	5.51E-49	-1.69E-46
MS.gene057869	MS.gene93638	0.819307	1.24E-52	-1.69E-46
MS.gene058010	MS.gene93638	0.808546	2.94E-50	-1.69E-46
MS.gene059128	MS.gene93638	0.815854	7.47E-52	-1.69E-46
MS.gene059842	MS.gene93638	0.814633	1.39E-51	-1.69E-46
MS.gene059937	MS.gene93638	0.805054	1.61E-49	-1.69E-46
MS.gene060011	MS.gene93638	0.810064	1.39E-50	-1.69E-46
MS.gene060137	MS.gene93638	-0.809475	1.86E-50	-1.69E-46
MS.gene060310	MS.gene93638	0.807921	3.99E-50	-1.69E-46
MS.gene060322	MS.gene93638	0.80626	8.97E-50	-1.69E-46
MS.gene060802	MS.gene93638	0.807108	5.94E-50	-1.69E-46
MS.gene060803	MS.gene93638	0.820934	5.28E-53	-1.69E-46
MS.gene060918	MS.gene93638	0.807169	5.76E-50	-1.69E-46
MS.gene060998	MS.gene93638	0.814116	1.81E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93638.t1	MTR_7g098290	95.050	101	5	0	1	101	1	101	3.50e-65	193
MS.gene93638.t1	MTR_1g069585	95.050	101	5	0	1	101	1	101	3.50e-65	193
MS.gene93638.t1	MTR_7g098290	94.186	86	5	0	16	101	1	86	5.09e-54	165
MS.gene93638.t1	MTR_1g026090	73.000	100	12	1	2	101	42	126	1.05e-42	138
MS.gene93638.t1	MTR_5g464620	82.000	50	9	0	13	62	15	64	6.46e-23	84.7
MS.gene93638.t1	MTR_5g054560	53.488	86	16	1	16	101	1	62	6.99e-22	82.8

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93638.t1	AT3G04400	92.079	101	8	1	101	1	101	1.34e-63	189
MS.gene93638.t1	AT2G33370	92.079	101	8	1	101	1	101	1.34e-63	189
MS.gene93638.t1	AT1G04480	92.079	101	8	1	101	1	101	1.34e-63	189
MS.gene93638.t1	AT3G04400	93.023	86	6	16	101	1	86	3.15e-53	163
MS.gene93638.t1	AT2G33370	93.023	86	6	16	101	1	86	3.15e-53	163

Find 34 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAACAAGTTCCGTATGTCAC+TGG	0.413902	6.2:+70847563	MS.gene93638:CDS
ACATGAAGACACCGTCCTTT+CGG	0.413999	6.2:-70847797	None:intergenic
CCTACCCTTGATTCCCTTCA+CGG	0.419579	6.2:-70847652	None:intergenic
AAAACATTCTTCCTGAGATC+AGG	0.427356	6.2:-70847738	None:intergenic
CGTCTTCCTTCAGCTTGTGC+TGG	0.450748	6.2:+70847681	MS.gene93638:CDS
TTAGGTCGTGGTGGTTCCGC+GGG	0.471837	6.2:+70847540	MS.gene93638:intron
CATCTCCGTGAAGGGAATCA+AGG	0.491257	6.2:+70847647	MS.gene93638:CDS
GGTGTCTTCATGTACTTTGA+AGG	0.499615	6.2:+70847807	MS.gene93638:CDS
CAGTTCACCGTCGCTGCCAC+TGG	0.502403	6.2:-70847591	None:intergenic
TTGTGCTGGTGACGTGGTGA+TGG	0.519017	6.2:+70847695	MS.gene93638:CDS
AGAAGGGAAAGCCTGATCTC+AGG	0.525390	6.2:+70847727	MS.gene93638:CDS
GATGTACAGATTCTTTGCTC+TGG	0.533111	6.2:-70847625	None:intergenic
TGCTGACGAACGATGACAGC+TGG	0.538722	6.2:-70847762	None:intergenic
ACACCGTCCTTTCGGCGCCA+TGG	0.546165	6.2:-70847789	None:intergenic
AACAAGTTCCGTATGTCACT+GGG	0.554811	6.2:+70847564	MS.gene93638:CDS
AAGCCATGGCGCCGAAAGGA+CGG	0.558400	6.2:+70847786	MS.gene93638:CDS
ATTAGGTCGTGGTGGTTCCG+CGG	0.570592	6.2:+70847539	MS.gene93638:intron
CTGGTAGACCCAGTGACATA+CGG	0.574663	6.2:-70847572	None:intergenic
GGTGATGGCTACTGTGAAGA+AGG	0.577572	6.2:+70847710	MS.gene93638:CDS
TTCAGCTTGTGCTGGTGACG+TGG	0.580455	6.2:+70847689	MS.gene93638:CDS
GGGTCTACCAGTGGCAGCGA+CGG	0.583652	6.2:+70847584	MS.gene93638:CDS
CTGTACATCATCTCCGTGAA+GGG	0.589573	6.2:+70847639	MS.gene93638:CDS
ATCTCCGTGAAGGGAATCAA+GGG	0.592804	6.2:+70847648	MS.gene93638:CDS
TCTGTACATCATCTCCGTGA+AGG	0.600106	6.2:+70847638	MS.gene93638:CDS
GCATAAGCCATGGCGCCGAA+AGG	0.608624	6.2:+70847782	MS.gene93638:CDS
ACGTCACCAGCACAAGCTGA+AGG	0.618315	6.2:-70847687	None:intergenic
AACGCAACCATGTCGAAGAG+AGG	0.636545	6.2:+70847439	None:intergenic
TCGTTCGTCAGCATAAGCCA+TGG	0.638983	6.2:+70847772	MS.gene93638:CDS
CCGTGAAGGGAATCAAGGGT+AGG	0.651559	6.2:+70847652	MS.gene93638:CDS
TATGTCACTGGGTCTACCAG+TGG	0.667978	6.2:+70847575	MS.gene93638:CDS
GTGATGGCTACTGTGAAGAA+GGG	0.669945	6.2:+70847711	MS.gene93638:CDS
GTACATACCTCTCTTCGACA+TGG	0.681454	6.2:-70847446	None:intergenic
TAGGTCGTGGTGGTTCCGCG+GGG	0.708397	6.2:+70847541	MS.gene93638:intron
CATACGGAACTTGTTCCCCG+CGG	0.790506	6.2:-70847556	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATACAATTGAATCTGAGAA+AGG	-	chr6.2:70847485-70847504	None:intergenic	25.0%
!	TGTATCCCTATTTTCTGATT+AGG	+	chr6.2:70847522-70847541	MS.gene93638:intron	30.0%
	AAAACATTCTTCCTGAGATC+AGG	-	chr6.2:70847741-70847760	None:intergenic	35.0%
	CACGACCTAATCAGAAAATA+GGG	-	chr6.2:70847530-70847549	None:intergenic	35.0%
	AACAAGTTCCGTATGTCACT+GGG	+	chr6.2:70847564-70847583	MS.gene93638:CDS	40.0%
	CCACGACCTAATCAGAAAAT+AGG	-	chr6.2:70847531-70847550	None:intergenic	40.0%
	GATGTACAGATTCTTTGCTC+TGG	-	chr6.2:70847628-70847647	None:intergenic	40.0%
	GGTGTCTTCATGTACTTTGA+AGG	+	chr6.2:70847807-70847826	MS.gene93638:CDS	40.0%
!	ATTTTCTGATTAGGTCGTGG+TGG	+	chr6.2:70847531-70847550	MS.gene93638:intron	40.0%
!	CCTATTTTCTGATTAGGTCG+TGG	+	chr6.2:70847528-70847547	MS.gene93638:intron	40.0%
	ACATGAAGACACCGTCCTTT+CGG	-	chr6.2:70847800-70847819	None:intergenic	45.0%
	ATCTCCGTGAAGGGAATCAA+GGG	+	chr6.2:70847648-70847667	MS.gene93638:CDS	45.0%
	CTGTACATCATCTCCGTGAA+GGG	+	chr6.2:70847639-70847658	MS.gene93638:CDS	45.0%
	GAACAAGTTCCGTATGTCAC+TGG	+	chr6.2:70847563-70847582	MS.gene93638:CDS	45.0%
	GTGATGGCTACTGTGAAGAA+GGG	+	chr6.2:70847711-70847730	MS.gene93638:CDS	45.0%
	TCTGTACATCATCTCCGTGA+AGG	+	chr6.2:70847638-70847657	MS.gene93638:CDS	45.0%
	AGAAGGGAAAGCCTGATCTC+AGG	+	chr6.2:70847727-70847746	MS.gene93638:CDS	50.0%
	CATCTCCGTGAAGGGAATCA+AGG	+	chr6.2:70847647-70847666	MS.gene93638:CDS	50.0%
	CCTACCCTTGATTCCCTTCA+CGG	-	chr6.2:70847655-70847674	None:intergenic	50.0%
	CTGGTAGACCCAGTGACATA+CGG	-	chr6.2:70847575-70847594	None:intergenic	50.0%
	TATGTCACTGGGTCTACCAG+TGG	+	chr6.2:70847575-70847594	MS.gene93638:CDS	50.0%
	TCGTTCGTCAGCATAAGCCA+TGG	+	chr6.2:70847772-70847791	MS.gene93638:CDS	50.0%
!	GGTGATGGCTACTGTGAAGA+AGG	+	chr6.2:70847710-70847729	MS.gene93638:CDS	50.0%
	ACGTCACCAGCACAAGCTGA+AGG	-	chr6.2:70847690-70847709	None:intergenic	55.0%
	ATTAGGTCGTGGTGGTTCCG+CGG	+	chr6.2:70847539-70847558	MS.gene93638:intron	55.0%
	CCGTGAAGGGAATCAAGGGT+AGG	+	chr6.2:70847652-70847671	MS.gene93638:CDS	55.0%
	CGTCTTCCTTCAGCTTGTGC+TGG	+	chr6.2:70847681-70847700	MS.gene93638:CDS	55.0%
	TGCTGACGAACGATGACAGC+TGG	-	chr6.2:70847765-70847784	None:intergenic	55.0%
!	CATACGGAACTTGTTCCCCG+CGG	-	chr6.2:70847559-70847578	None:intergenic	55.0%
!	TTCAGCTTGTGCTGGTGACG+TGG	+	chr6.2:70847689-70847708	MS.gene93638:CDS	55.0%
!	TTGTGCTGGTGACGTGGTGA+TGG	+	chr6.2:70847695-70847714	MS.gene93638:CDS	55.0%
	TTAGGTCGTGGTGGTTCCGC+GGG	+	chr6.2:70847540-70847559	MS.gene93638:intron	60.0%
!	AAGCCATGGCGCCGAAAGGA+CGG	+	chr6.2:70847786-70847805	MS.gene93638:CDS	60.0%
!	GCATAAGCCATGGCGCCGAA+AGG	+	chr6.2:70847782-70847801	MS.gene93638:CDS	60.0%
	ACACCGTCCTTTCGGCGCCA+TGG	-	chr6.2:70847792-70847811	None:intergenic	65.0%
	CAGTTCACCGTCGCTGCCAC+TGG	-	chr6.2:70847594-70847613	None:intergenic	65.0%
	GGGTCTACCAGTGGCAGCGA+CGG	+	chr6.2:70847584-70847603	MS.gene93638:CDS	65.0%
	TAGGTCGTGGTGGTTCCGCG+GGG	+	chr6.2:70847541-70847560	MS.gene93638:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	70847448	70847860	70847448	ID=MS.gene93638
chr6.2	mRNA	70847448	70847860	70847448	ID=MS.gene93638.t1;Parent=MS.gene93638
chr6.2	exon	70847448	70847460	70847448	ID=MS.gene93638.t1.exon1;Parent=MS.gene93638.t1
chr6.2	CDS	70847448	70847460	70847448	ID=cds.MS.gene93638.t1;Parent=MS.gene93638.t1
chr6.2	exon	70847544	70847860	70847544	ID=MS.gene93638.t1.exon2;Parent=MS.gene93638.t1
chr6.2	CDS	70847544	70847860	70847544	ID=cds.MS.gene93638.t1;Parent=MS.gene93638.t1

Gene Sequence

>MS.gene93638

ATGTCGAAGAGAGGTATGTACAGTGATTGTGAATCCTTTCTCAGATTCAATTGTATTGTTCTCTTTTTCTGATATGTATCCCTATTTTCTGATTAGGTCGTGGTGGTTCCGCGGGGAACAAGTTCCGTATGTCACTGGGTCTACCAGTGGCAGCGACGGTGAACTGTGCTGATAACACCAGAGCAAAGAATCTGTACATCATCTCCGTGAAGGGAATCAAGGGTAGGTTGAATCGTCTTCCTTCAGCTTGTGCTGGTGACGTGGTGATGGCTACTGTGAAGAAGGGAAAGCCTGATCTCAGGAAGAATGTTTTGCCAGCTGTCATCGTTCGTCAGCATAAGCCATGGCGCCGAAAGGACGGTGTCTTCATGTACTTTGAAGGTAACAAACGCTGTTTTGATTCATGTTTTTGA

Protein sequence

>MS.gene93638.t1

MSKRGRGGSAGNKFRMSLGLPVAATVNCADNTRAKNLYIISVKGIKGRLNRLPSACAGDVVMATVKKGKPDLRKNVLPAVIVRQHKPWRRKDGVFMYFEGNKRCFDSCF