AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93985


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93985.t1 MTR_6g070920 91.824 159 1 1 1 147 72 230 4.45e-105 299
MS.gene93985.t1 MTR_6g070920 87.407 135 5 1 1 123 72 206 5.16e-83 243
MS.gene93985.t1 MTR_3g065330 55.696 158 53 4 1 142 10 166 3.17e-54 169
MS.gene93985.t1 MTR_1g032290 54.717 159 56 4 1 144 1 158 8.86e-54 167
MS.gene93985.t1 MTR_8g105140 54.037 161 58 4 1 146 1 160 2.06e-53 166
MS.gene93985.t1 MTR_5g078020 51.656 151 55 3 8 142 16 164 5.82e-46 148
MS.gene93985.t1 MTR_6g070920 81.319 91 5 1 1 79 72 162 1.46e-45 146
MS.gene93985.t1 MTR_3g065330 55.556 135 43 4 1 119 32 165 4.65e-43 140
MS.gene93985.t1 MTR_8g039690 41.509 159 72 3 2 139 1 159 1.78e-33 116
MS.gene93985.t1 MTR_7g084380 42.331 163 70 4 2 140 1 163 1.80e-32 114
MS.gene93985.t1 MTR_7g084380 40.288 139 59 4 26 140 21 159 2.40e-23 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93985.t1 AT1G18300 55.414 157 53 4 1 141 40 195 2.26e-55 172
MS.gene93985.t1 AT1G73540 55.414 157 53 4 1 143 41 194 3.93e-53 167
MS.gene93985.t1 AT1G14860 55.128 156 55 3 1 142 1 155 8.93e-53 165
MS.gene93985.t1 AT2G01670 50.932 161 64 3 1 147 6 165 3.50e-50 159
MS.gene93985.t1 AT1G12880 42.169 166 69 4 2 140 1 166 5.44e-33 115
MS.gene93985.t1 AT3G26690 40.120 167 72 4 2 140 1 167 8.81e-30 107
MS.gene93985.t1 AT3G26690 40.120 167 72 4 2 140 1 167 8.81e-30 107
MS.gene93985.t1 AT3G12600 43.750 160 67 4 2 138 1 160 1.86e-26 98.2
MS.gene93985.t1 AT3G12600 43.243 148 61 4 14 138 4 151 1.81e-22 87.8

Find 33 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCTTATGTTCATTTC+TGG 0.092026 6.2:+73517402 None:intergenic
TGCAGAGTAATTTGGGTAAA+TGG 0.215433 6.2:-73517506 MS.gene93985:intron
TGAACATAAGAAGAAGAAAA+TGG 0.253808 6.2:-73517395 MS.gene93985:CDS
ATAGAAGAAGCTGGAGTTAT+TGG 0.256499 6.2:-73517641 MS.gene93985:CDS
AATAACTCCAGCTTCTTCTA+TGG 0.282235 6.2:+73517643 None:intergenic
TTAGCAAGGAGTTGGATAAT+TGG 0.322855 6.2:-73517425 MS.gene93985:CDS
GAAGGAAGCTTTAGATGAAT+TGG 0.336584 6.2:-73516989 MS.gene93985:CDS
ACCTAAAACTTAGATTGAAT+TGG 0.341917 6.2:+73516954 None:intergenic
TGCAAATGTCTACCAGTTCT+AGG 0.360990 6.2:+73518383 None:intergenic
GCTTCCTTCATCCAAGCATA+TGG 0.390570 6.2:+73517003 None:intergenic
AAGAAATATGCCCATATGCT+TGG 0.435806 6.2:-73517014 MS.gene93985:CDS
TGTTTGGTTTCTCCTAGAAC+TGG 0.445974 6.2:-73518395 MS.gene93985:CDS
ATTGGAGAATATGATGTGTT+TGG 0.449206 6.2:-73518411 None:intergenic
GAACATAAGAAGAAGAAAAT+GGG 0.454169 6.2:-73517394 MS.gene93985:intron
GTTCCCTTTGCTTGTTAGCA+AGG 0.472162 6.2:-73517439 MS.gene93985:CDS
TTTGCTTGTTAGCAAGGAGT+TGG 0.481309 6.2:-73517433 MS.gene93985:CDS
AAAGAATGGAACTCAAGAAA+AGG 0.494824 6.2:-73517771 MS.gene93985:CDS
TCTCAAAGCAGCTTGTTCCA+TGG 0.496073 6.2:+73517670 None:intergenic
AGAGAAACCATAGAAGAAGC+TGG 0.505150 6.2:-73517650 MS.gene93985:CDS
AACTCAAGAAAAGGAAATAG+AGG 0.509278 6.2:-73517762 MS.gene93985:CDS
AACTCCTTGCTAACAAGCAA+AGG 0.516565 6.2:+73517435 None:intergenic
CTTCCTTCATCCAAGCATAT+GGG 0.522789 6.2:+73517004 None:intergenic
ACAAGGATGTCGTCAAGTTG+TGG 0.541451 6.2:-73518351 MS.gene93985:CDS
CATGTATCCTTCATGCATAG+TGG 0.542137 6.2:+73517461 None:intergenic
CAAGGATGTCGTCAAGTTGT+GGG 0.550727 6.2:-73518350 MS.gene93985:CDS
ACTCCTTGCTAACAAGCAAA+GGG 0.551729 6.2:+73517436 None:intergenic
ATGCCCATATGCTTGGATGA+AGG 0.557447 6.2:-73517007 MS.gene93985:CDS
ATCCTTCATGCATAGTGGGT+TGG 0.562217 6.2:+73517466 None:intergenic
ATGTATCCTTCATGCATAGT+GGG 0.569535 6.2:+73517462 None:intergenic
CATTTGCAACGTTATGAACA+AGG 0.571762 6.2:-73518368 MS.gene93985:CDS
CGCCAACCCACTATGCATGA+AGG 0.576057 6.2:-73517468 MS.gene93985:CDS
AAGGATGTCGTCAAGTTGTG+GGG 0.655005 6.2:-73518349 MS.gene93985:intron
TGTAATCATGCAGTTGACTG+TGG 0.660929 6.2:-73517046 MS.gene93985:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATAAAAGTTGAATAT+AGG + chr6.2:73518011-73518030 None:intergenic 10.0%
!!! ATTTTTCATGAAATAATATT+TGG - chr6.2:73517797-73517816 MS.gene93985:intron 10.0%
!!! GAAATTTTTTGAAATTTTTA+AGG - chr6.2:73517287-73517306 MS.gene93985:intron 10.0%
!!! TTTTTTTTTTTTTCTCAATT+GGG - chr6.2:73517220-73517239 MS.gene93985:intron 10.0%
!!! TTTTTTTTTTTTTTCTCAAT+TGG - chr6.2:73517219-73517238 MS.gene93985:intron 10.0%
!! TTACAAATGAAAGAAATATT+AGG + chr6.2:73518303-73518322 None:intergenic 15.0%
!!! AAAATTTTGAATTGAATATG+AGG - chr6.2:73517173-73517192 MS.gene93985:intron 15.0%
!!! AAATTTTGAATTGAATATGA+GGG - chr6.2:73517174-73517193 MS.gene93985:intron 15.0%
!!! AAGTTTTGATTTTTAAGATT+TGG - chr6.2:73517331-73517350 MS.gene93985:intron 15.0%
!!! AATTTGAATTTGATTTATGA+GGG - chr6.2:73517501-73517520 MS.gene93985:CDS 15.0%
!!! GACAATTTATGAAATTTTTA+AGG - chr6.2:73517128-73517147 MS.gene93985:intron 15.0%
!!! TAATTTGAATTTGATTTATG+AGG - chr6.2:73517500-73517519 MS.gene93985:CDS 15.0%
!!! TGTAATAACTTTTTTAGATT+TGG - chr6.2:73517768-73517787 MS.gene93985:CDS 15.0%
!! ATGATTCAACAATGAATAAA+AGG + chr6.2:73517436-73517455 None:intergenic 20.0%
!! TCTTAATTAAGTAGCATTAT+TGG - chr6.2:73518250-73518269 MS.gene93985:intron 20.0%
!! TGATTCAACAATGAATAAAA+GGG + chr6.2:73517435-73517454 None:intergenic 20.0%
!! TTTATAAATGACACTTGATT+AGG + chr6.2:73518184-73518203 None:intergenic 20.0%
!! TTTATTCATTGTTGAATCAT+TGG - chr6.2:73517436-73517455 MS.gene93985:CDS 20.0%
!!! AAGTTTGGATTTTTATTGTT+TGG - chr6.2:73517250-73517269 MS.gene93985:intron 20.0%
!!! ATATTGTGATTTGTTCTTAT+GGG - chr6.2:73517061-73517080 MS.gene93985:intron 20.0%
!!! ATTTTTAAGGTTTTTTCAGT+TGG - chr6.2:73517300-73517319 MS.gene93985:intron 20.0%
!!! GACAATTTTTGATAACTTTA+AGG - chr6.2:73517196-73517215 MS.gene93985:intron 20.0%
!!! TTTTAAGGTTTTTCTCAATT+GGG - chr6.2:73517143-73517162 MS.gene93985:intron 20.0%
!!! TTTTAATGTTTTTCTCAAGT+GGG - chr6.2:73517383-73517402 MS.gene93985:intron 20.0%
!!! TTTTTAAGGTTTTTCTCAAT+TGG - chr6.2:73517142-73517161 MS.gene93985:intron 20.0%
!!! TTTTTAAGGTTTTTTCAGTT+GGG - chr6.2:73517301-73517320 MS.gene93985:intron 20.0%
!!! TTTTTAATGTTTTTCTCAAG+TGG - chr6.2:73517382-73517401 MS.gene93985:intron 20.0%
! AGATGAATTGACATTTGAAT+AGG + chr6.2:73518216-73518235 None:intergenic 25.0%
! CCTTATAGATACAAAAAGAA+TGG - chr6.2:73517579-73517598 MS.gene93985:intron 25.0%
! CTTATGGGTTTCTTAAAAAT+TGG - chr6.2:73517076-73517095 MS.gene93985:intron 25.0%
! GAACATAAGAAGAAGAAAAT+GGG - chr6.2:73517970-73517989 MS.gene93985:intron 25.0%
! TGAACATAAGAAGAAGAAAA+TGG - chr6.2:73517969-73517988 MS.gene93985:intron 25.0%
!! CCATTCTTTTTGTATCTATA+AGG + chr6.2:73517582-73517601 None:intergenic 25.0%
!!! AAAAAAGTATTATGTCTGAG+CGG + chr6.2:73518112-73518131 None:intergenic 25.0%
!!! GATATTGTGATTTGTTCTTA+TGG - chr6.2:73517060-73517079 MS.gene93985:intron 25.0%
!!! GTTAATCTAACTTTGTTTTG+TGG - chr6.2:73517532-73517551 MS.gene93985:intron 25.0%
!!! TAACTTTGTTTTGTGGTTTT+TGG - chr6.2:73517539-73517558 MS.gene93985:intron 25.0%
!!! TTTAAGGTTTTTCTCAATTG+GGG - chr6.2:73517144-73517163 MS.gene93985:intron 25.0%
!!! TTTTAAGGTTTTTTCAGTTG+GGG - chr6.2:73517302-73517321 MS.gene93985:intron 25.0%
AAAGAATGGAACTCAAGAAA+AGG - chr6.2:73517593-73517612 MS.gene93985:intron 30.0%
AACTCAAGAAAAGGAAATAG+AGG - chr6.2:73517602-73517621 MS.gene93985:intron 30.0%
CAATTGGGTTTCTTAAAGTT+TGG - chr6.2:73517235-73517254 MS.gene93985:intron 30.0%
CTTCTTCTTATGTTCATTTC+TGG + chr6.2:73517965-73517984 None:intergenic 30.0%
TTCATTGTTGAATCATTGGA+AGG - chr6.2:73517440-73517459 MS.gene93985:CDS 30.0%
! CTTTTGATAAGTGCTCAAAA+GGG - chr6.2:73517627-73517646 MS.gene93985:intron 30.0%
! GTTTTCTTGCAGAGTAATTT+GGG - chr6.2:73517851-73517870 MS.gene93985:intron 30.0%
! TCTTTTGATAAGTGCTCAAA+AGG - chr6.2:73517626-73517645 MS.gene93985:intron 30.0%
! TGTTTTCTTGCAGAGTAATT+TGG - chr6.2:73517850-73517869 MS.gene93985:intron 30.0%
! TTGTTTGGAATTGTAGATGA+TGG - chr6.2:73517265-73517284 MS.gene93985:intron 30.0%
AAGAAATATGCCCATATGCT+TGG - chr6.2:73518350-73518369 MS.gene93985:CDS 35.0%
AATAACTCCAGCTTCTTCTA+TGG + chr6.2:73517724-73517743 None:intergenic 35.0%
ATAGAAGAAGCTGGAGTTAT+TGG - chr6.2:73517723-73517742 MS.gene93985:intron 35.0%
ATGTATCCTTCATGCATAGT+GGG + chr6.2:73517905-73517924 None:intergenic 35.0%
CATTTGCAACGTTATGAACA+AGG - chr6.2:73516996-73517015 MS.gene93985:CDS 35.0%
TGCAGAGTAATTTGGGTAAA+TGG - chr6.2:73517858-73517877 MS.gene93985:intron 35.0%
TTAGCAAGGAGTTGGATAAT+TGG - chr6.2:73517939-73517958 MS.gene93985:intron 35.0%
TTGGGAAAAAGATGAAACCA+TGG - chr6.2:73517677-73517696 MS.gene93985:CDS 35.0%
!! GAAGGAAGCTTTAGATGAAT+TGG - chr6.2:73518375-73518394 MS.gene93985:CDS 35.0%
AACTCCTTGCTAACAAGCAA+AGG + chr6.2:73517932-73517951 None:intergenic 40.0%
ACTCCTTGCTAACAAGCAAA+GGG + chr6.2:73517931-73517950 None:intergenic 40.0%
AGAGAAACCATAGAAGAAGC+TGG - chr6.2:73517714-73517733 MS.gene93985:intron 40.0%
AGTGGAATGCAGTTTCCAAA+AGG - chr6.2:73517651-73517670 MS.gene93985:CDS 40.0%
ATAAGTGCTCAAAAGGGTAG+TGG - chr6.2:73517633-73517652 MS.gene93985:CDS 40.0%
ATGACACTTGATTAGGCTCA+AGG + chr6.2:73518177-73518196 None:intergenic 40.0%
CATGTATCCTTCATGCATAG+TGG + chr6.2:73517906-73517925 None:intergenic 40.0%
CTTCCTTCATCCAAGCATAT+GGG + chr6.2:73518363-73518382 None:intergenic 40.0%
TGCAAATGTCTACCAGTTCT+AGG + chr6.2:73516984-73517003 None:intergenic 40.0%
TGTAATCATGCAGTTGACTG+TGG - chr6.2:73518318-73518337 MS.gene93985:intron 40.0%
! TCTTTTTCCCAACCTCCTTT+TGG + chr6.2:73517669-73517688 None:intergenic 40.0%
! TGTTTGGTTTCTCCTAGAAC+TGG - chr6.2:73516969-73516988 MS.gene93985:CDS 40.0%
! TTTGCTTGTTAGCAAGGAGT+TGG - chr6.2:73517931-73517950 MS.gene93985:intron 40.0%
ATGCCCATATGCTTGGATGA+AGG - chr6.2:73518357-73518376 MS.gene93985:CDS 45.0%
GCAGTTTCCAAAAGGAGGTT+GGG - chr6.2:73517659-73517678 MS.gene93985:CDS 45.0%
GCTTCCTTCATCCAAGCATA+TGG + chr6.2:73518364-73518383 None:intergenic 45.0%
GGAATGCAGTTTCCAAAAGG+AGG - chr6.2:73517654-73517673 MS.gene93985:CDS 45.0%
TGCAGTTTCCAAAAGGAGGT+TGG - chr6.2:73517658-73517677 MS.gene93985:CDS 45.0%
! AAGGATGTCGTCAAGTTGTG+GGG - chr6.2:73517015-73517034 MS.gene93985:CDS 45.0%
! ACAAGGATGTCGTCAAGTTG+TGG - chr6.2:73517013-73517032 MS.gene93985:CDS 45.0%
! CAAGGATGTCGTCAAGTTGT+GGG - chr6.2:73517014-73517033 MS.gene93985:CDS 45.0%
! GTTCCCTTTGCTTGTTAGCA+AGG - chr6.2:73517925-73517944 MS.gene93985:intron 45.0%
! TCTCAAAGCAGCTTGTTCCA+TGG + chr6.2:73517697-73517716 None:intergenic 45.0%
!! ATCCTTCATGCATAGTGGGT+TGG + chr6.2:73517901-73517920 None:intergenic 45.0%
TTATGTCGCAGCGCAGATTG+AGG - chr6.2:73518130-73518149 MS.gene93985:intron 50.0%
CGCCAACCCACTATGCATGA+AGG - chr6.2:73517896-73517915 MS.gene93985:intron 55.0%
TGTCTGAGCGGCTACGATTG+AGG + chr6.2:73518100-73518119 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 73516963 73518423 73516963 ID=MS.gene93985
chr6.2 mRNA 73516963 73518423 73516963 ID=MS.gene93985.t1;Parent=MS.gene93985
chr6.2 exon 73518350 73518423 73518350 ID=MS.gene93985.t1.exon1;Parent=MS.gene93985.t1
chr6.2 CDS 73518350 73518423 73518350 ID=cds.MS.gene93985.t1;Parent=MS.gene93985.t1
chr6.2 exon 73517735 73517814 73517735 ID=MS.gene93985.t1.exon2;Parent=MS.gene93985.t1
chr6.2 CDS 73517735 73517814 73517735 ID=cds.MS.gene93985.t1;Parent=MS.gene93985.t1
chr6.2 exon 73517631 73517698 73517631 ID=MS.gene93985.t1.exon3;Parent=MS.gene93985.t1
chr6.2 CDS 73517631 73517698 73517631 ID=cds.MS.gene93985.t1;Parent=MS.gene93985.t1
chr6.2 exon 73517395 73517523 73517395 ID=MS.gene93985.t1.exon4;Parent=MS.gene93985.t1
chr6.2 CDS 73517395 73517523 73517395 ID=cds.MS.gene93985.t1;Parent=MS.gene93985.t1
chr6.2 exon 73516963 73517055 73516963 ID=MS.gene93985.t1.exon5;Parent=MS.gene93985.t1
chr6.2 CDS 73516963 73517055 73516963 ID=cds.MS.gene93985.t1;Parent=MS.gene93985.t1
Gene Sequence

>MS.gene93985

ATGATGTGTTTGGTTTCTCCTAGAACTGGTAGACATTTGCAACGTTATGAACAAGGATGTCGTCAAGTTGTGGGGTGTGTTTCTGTTTCTCTCTCTTGATATTGTGATTTGTTCTTATGGGTTTCTTAAAAATTGGATTTTTAATGTTTAATATTGAATATAATTGACAATTTATGAAATTTTTAAGGTTTTTCTCAATTGGGGTTTCTTAAAATTTTGAATTGAATATGAGGGACAATTTTTGATAACTTTAAGGTTTTTTTTTTTTTTCTCAATTGGGTTTCTTAAAGTTTGGATTTTTATTGTTTGGAATTGTAGATGATGGAAATTTTTTGAAATTTTTAAGGTTTTTTCAGTTGGGGTTTTTGAAGTTTTGATTTTTAAGATTTGGAATTGAAGATGATGAACAATTTTTGAAATTTTTAATGTTTTTCTCAAGTGGGTTTTTTTCTAATGATTTTAAAGTATACCCTTTTATTCATTGTTGAATCATTGGAAGGAAAAGTGAAAAGTCAATTTTTTTTTTTTTTGTGAATTTAATTTGAATTTGATTTATGAGGGTTTGAATTGTTAATCTAACTTTGTTTTGTGGTTTTTGGAATTGAACAGATGCATACCTTATAGATACAAAAAGAATGGAACTCAAGAAAAGGAAATAGAGGTTCTTTTGATAAGTGCTCAAAAGGGTAGTGGAATGCAGTTTCCAAAAGGAGGTTGGGAAAAAGATGAAACCATGGAACAAGCTGCTTTGAGAGAAACCATAGAAGAAGCTGGAGTTATTGGAAGTGTTGAAGTAAGTATTTATTGTAATAACTTTTTTAGATTTGGTTTTTCATTTTTCATGAAATAATATTTGGTACTTTGATTCTAACTTTGTGTATTATGATTGTTTTCTTGCAGAGTAATTTGGGTAAATGGTACTATAAGAGCAAACGCCAACCCACTATGCATGAAGGATACATGTTCCCTTTGCTTGTTAGCAAGGAGTTGGATAATTGGCCAGAAATGAACATAAGAAGAAGAAAATGGGTAAGTGTATATGAGACCTATATTCAACTTTTATAATATTCTAATCATGTTTGCGACACGATTCTTGCTATTGCAGAGAATTGCAGTCAAATACAACTTTTGCAACCTCAATCGTAGCCGCTCAGACATAATACTTTTTTATGTCGCAGCGCAGATTGAGGTTGCATACATGATTTTCAAAACCTTGAGCCTAATCAAGTGTCATTTATAAATTATTGTGTCCTATTCAAATGTCAATTCATCTTGCAAATCGCAATATCTTAATTAAGTAGCATTATTGGTTTACTATTATTATTTTTTGTTAGTGTCCTAATATTTCTTTCATTTGTAATCATGCAGTTGACTGTGGATGAAGCAAAAGAAATATGCCCATATGCTTGGATGAAGGAAGCTTTAGATGAATTGGTTAGTAGACAAACCAATTCAATCTAA

Protein sequence

>MS.gene93985.t1

MMCLVSPRTGRHLQRYEQGCRQVVGCIPYRYKKNGTQEKEIEVLLISAQKGNETMEQAALRETIEEAGVIGSVESNLGKWYYKSKRQPTMHEGYMFPLLVSKELDNWPEMNIRRRKWLTVDEAKEICPYAWMKEALDELVSRQTNSI