Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056901.t1 | XP_013456188.1 | 56.6 | 152 | 66 | 0 | 6 | 157 | 3 | 154 | 8.80E-32 | 146.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056901.t1 | A0A072UKD9 | 56.6 | 152 | 66 | 0 | 6 | 157 | 3 | 154 | 6.3e-32 | 146.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049786 | MS.gene056901 | 0.813649 | 2.30E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene056901 | 0.811714 | 6.10E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene056901 | 0.800747 | 1.25E-48 | -1.69E-46 |
| MS.gene055313 | MS.gene056901 | 0.806473 | 8.09E-50 | -1.69E-46 |
| MS.gene055603 | MS.gene056901 | 0.878251 | 3.27E-69 | -1.69E-46 |
| MS.gene056787 | MS.gene056901 | 0.809749 | 1.62E-50 | -1.69E-46 |
| MS.gene056901 | MS.gene057360 | 0.802324 | 5.92E-49 | -1.69E-46 |
| MS.gene056901 | MS.gene065072 | 0.809676 | 1.68E-50 | -1.69E-46 |
| MS.gene056901 | MS.gene066319 | 0.815471 | 9.09E-52 | -1.69E-46 |
| MS.gene056901 | MS.gene066321 | 0.812322 | 4.49E-51 | -1.69E-46 |
| MS.gene056901 | MS.gene07339 | 0.804516 | 2.08E-49 | -1.69E-46 |
| MS.gene056901 | MS.gene073753 | 0.804001 | 2.66E-49 | -1.69E-46 |
| MS.gene056901 | MS.gene07608 | 0.801711 | 7.91E-49 | -1.69E-46 |
| MS.gene056901 | MS.gene09111 | 0.811045 | 8.52E-51 | -1.69E-46 |
| MS.gene056901 | MS.gene20886 | 0.817854 | 2.66E-52 | -1.69E-46 |
| MS.gene056901 | MS.gene24189 | 0.802332 | 5.90E-49 | -1.69E-46 |
| MS.gene056901 | MS.gene25942 | 0.800724 | 1.26E-48 | -1.69E-46 |
| MS.gene056901 | MS.gene40991 | 0.810863 | 9.33E-51 | -1.69E-46 |
| MS.gene056901 | MS.gene42128 | 0.830021 | 3.71E-55 | -1.69E-46 |
| MS.gene056901 | MS.gene54904 | 0.823037 | 1.72E-53 | -1.69E-46 |
| MS.gene056901 | MS.gene56039 | 0.806271 | 8.92E-50 | -1.69E-46 |
| MS.gene056901 | MS.gene61257 | 0.810997 | 8.72E-51 | -1.69E-46 |
| MS.gene056901 | MS.gene64191 | 0.806277 | 8.90E-50 | -1.69E-46 |
| MS.gene056901 | MS.gene69821 | 0.811072 | 8.41E-51 | -1.69E-46 |
| MS.gene056901 | MS.gene70282 | 0.806391 | 8.41E-50 | -1.69E-46 |
| MS.gene056901 | MS.gene72621 | 0.812783 | 3.56E-51 | -1.69E-46 |
| MS.gene056901 | MS.gene74900 | 0.806502 | 7.97E-50 | -1.69E-46 |
| MS.gene056901 | MS.gene75605 | 0.810773 | 9.76E-51 | -1.69E-46 |
| MS.gene056901 | MS.gene75781 | 0.809643 | 1.71E-50 | -1.69E-46 |
| MS.gene056901 | MS.gene76248 | 0.80207 | 6.68E-49 | -1.69E-46 |
| MS.gene056901 | MS.gene81831 | 0.81589 | 7.33E-52 | -1.69E-46 |
| MS.gene056901 | MS.gene82600 | 0.801967 | 7.01E-49 | -1.69E-46 |
| MS.gene056901 | MS.gene84485 | 0.801512 | 8.70E-49 | -1.69E-46 |
| MS.gene056901 | MS.gene85246 | 0.803464 | 3.44E-49 | -1.69E-46 |
| MS.gene056901 | MS.gene93985 | 0.816354 | 5.77E-52 | -1.69E-46 |
| MS.gene056901 | MS.gene95305 | 0.806496 | 8.00E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056901.t1 | MTR_4g065050 | 71.034 | 145 | 42 | 0 | 13 | 157 | 10 | 154 | 3.33e-69 | 206 |
| MS.gene056901.t1 | MTR_4g065050 | 67.797 | 118 | 38 | 0 | 26 | 143 | 1 | 118 | 6.66e-53 | 164 |
| MS.gene056901.t1 | MTR_8g089030 | 47.727 | 88 | 43 | 2 | 7 | 91 | 38 | 125 | 9.98e-15 | 70.9 |
| MS.gene056901.t1 | MTR_8g089030 | 48.101 | 79 | 39 | 2 | 15 | 91 | 470 | 548 | 3.38e-11 | 60.8 |
| MS.gene056901.t1 | MTR_8g089030 | 47.727 | 88 | 43 | 2 | 7 | 91 | 38 | 125 | 1.17e-14 | 70.5 |
| MS.gene056901.t1 | MTR_8g089030 | 48.101 | 79 | 39 | 2 | 15 | 91 | 470 | 548 | 4.09e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGGTCCATTATGTAAAA+TGG | 0.330791 | 4.2:+40098784 | MS.gene056901:CDS |
| GGAAGAGGATATGTCTCTTC+AGG | 0.339040 | 4.2:+40098646 | MS.gene056901:CDS |
| GAACAACCGGAGCATCTTGC+AGG | 0.385771 | 4.2:+40098767 | MS.gene056901:CDS |
| TGCATTAATTGCGGAACAAC+CGG | 0.397610 | 4.2:+40098754 | MS.gene056901:CDS |
| GAAGAAACGGATCATATTCC+AGG | 0.409810 | 4.2:+40098875 | MS.gene056901:CDS |
| TGAATTGATTGCAGCCGAAT+TGG | 0.427925 | 4.2:+40098625 | MS.gene056901:CDS |
| TCTTCAGGATTCATTGAGAA+AGG | 0.440370 | 4.2:+40098661 | MS.gene056901:CDS |
| AGACATATCCTCTTCCAATT+CGG | 0.468675 | 4.2:-40098639 | None:intergenic |
| TGAATTTATTGAGGAAGAAA+CGG | 0.471592 | 4.2:+40098862 | MS.gene056901:CDS |
| CAATATTGATGAATTTATTG+AGG | 0.493374 | 4.2:+40098853 | MS.gene056901:CDS |
| TTCCGAGAAGTCCATGGAAA+AGG | 0.505472 | 4.2:+40098382 | MS.gene056901:CDS |
| AATGGACCTGCAAGATGCTC+CGG | 0.528367 | 4.2:-40098773 | None:intergenic |
| ATTTGTCAATGAATTGTACA+TGG | 0.532546 | 4.2:+40098943 | MS.gene056901:CDS |
| GATTGCAGCCGAATTGGAAG+AGG | 0.533785 | 4.2:+40098631 | MS.gene056901:CDS |
| CAACATTTCCGAGAAGTCCA+TGG | 0.547363 | 4.2:+40098376 | MS.gene056901:CDS |
| GTCAATGAATTGTACATGGA+TGG | 0.549535 | 4.2:+40098947 | MS.gene056901:CDS |
| AAGGTATGCTGCTTTGTATG+TGG | 0.584948 | 4.2:+40098976 | MS.gene056901:CDS |
| TGTACATGGATGGACAAGAA+AGG | 0.594997 | 4.2:+40098957 | MS.gene056901:CDS |
| GTACATGAAAGCGAGGAAAG+AGG | 0.620900 | 4.2:+40098817 | MS.gene056901:CDS |
| CTAAAATGTACATGAAAGCG+AGG | 0.632405 | 4.2:+40098810 | MS.gene056901:CDS |
| GGAGATGGATGCATTAATTG+CGG | 0.639286 | 4.2:+40098745 | MS.gene056901:CDS |
| TTGTATGTGGAGAAGTCTAG+TGG | 0.643539 | 4.2:+40098989 | MS.gene056901:CDS |
| TGTATGTGGAGAAGTCTAGT+GGG | 0.662396 | 4.2:+40098990 | MS.gene056901:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACTGTAAGAAGTTAAA+AGG | - | chr4.2:40099059-40099078 | None:intergenic | 20.0% |
| !! | AAAACTGTAAGAAGTTAAAA+GGG | - | chr4.2:40099058-40099077 | None:intergenic | 20.0% |
| !! | CAATATTGATGAATTTATTG+AGG | + | chr4.2:40098853-40098872 | MS.gene056901:CDS | 20.0% |
| !!! | AACATTTTTTTTTCAAAAGC+AGG | - | chr4.2:40098456-40098475 | None:intergenic | 20.0% |
| ! | AAAAAAAATGTTTCTTGCTG+TGG | + | chr4.2:40098464-40098483 | MS.gene056901:intron | 25.0% |
| ! | ATTTGTCAATGAATTGTACA+TGG | + | chr4.2:40098943-40098962 | MS.gene056901:CDS | 25.0% |
| ! | TGAATTTATTGAGGAAGAAA+CGG | + | chr4.2:40098862-40098881 | MS.gene056901:CDS | 25.0% |
| !! | AAAAAAATGTTTCTTGCTGT+GGG | + | chr4.2:40098465-40098484 | MS.gene056901:intron | 25.0% |
| !! | TTAGTGACCATTTTACATAA+TGG | - | chr4.2:40098794-40098813 | None:intergenic | 25.0% |
| AACTTGAATTTGGCTGTAAA+TGG | + | chr4.2:40098541-40098560 | MS.gene056901:intron | 30.0% | |
| AGAAGAGAATAATAAGCAAC+CGG | + | chr4.2:40098516-40098535 | MS.gene056901:intron | 30.0% | |
| ! | ACAGTTTTTCTTGTCACTAT+AGG | + | chr4.2:40099070-40099089 | MS.gene056901:intron | 30.0% |
| !! | TACTATTTTGAAAGAGGAGA+TGG | + | chr4.2:40098730-40098749 | MS.gene056901:CDS | 30.0% |
| !! | TTACGCTACTATTTTGAAAG+AGG | + | chr4.2:40098724-40098743 | MS.gene056901:CDS | 30.0% |
| AAAAACCCTAAAAGAGAACG+AGG | + | chr4.2:40098700-40098719 | MS.gene056901:CDS | 35.0% | |
| AGACATATCCTCTTCCAATT+CGG | - | chr4.2:40098642-40098661 | None:intergenic | 35.0% | |
| CTAAAATGTACATGAAAGCG+AGG | + | chr4.2:40098810-40098829 | MS.gene056901:CDS | 35.0% | |
| GAATTTGGCTGTAAATGGAT+GGG | + | chr4.2:40098546-40098565 | MS.gene056901:intron | 35.0% | |
| GTCAATGAATTGTACATGGA+TGG | + | chr4.2:40098947-40098966 | MS.gene056901:CDS | 35.0% | |
| TCTTCAGGATTCATTGAGAA+AGG | + | chr4.2:40098661-40098680 | MS.gene056901:CDS | 35.0% | |
| TGAATTTGGCTGTAAATGGA+TGG | + | chr4.2:40098545-40098564 | MS.gene056901:intron | 35.0% | |
| TGCAGGTCCATTATGTAAAA+TGG | + | chr4.2:40098784-40098803 | MS.gene056901:CDS | 35.0% | |
| TGCTTACTAGAAACTGTTTG+TGG | + | chr4.2:40098588-40098607 | MS.gene056901:intron | 35.0% | |
| ! | ACAGCCAAATTCAAGTTTAC+CGG | - | chr4.2:40098538-40098557 | None:intergenic | 35.0% |
| ! | ATCTCTATTTTCAATGCACC+TGG | - | chr4.2:40098896-40098915 | None:intergenic | 35.0% |
| ! | GTAAACCTCGTTCTCTTTTA+GGG | - | chr4.2:40098708-40098727 | None:intergenic | 35.0% |
| GAAGAAACGGATCATATTCC+AGG | + | chr4.2:40098875-40098894 | MS.gene056901:CDS | 40.0% | |
| GCAACCGGTAAACTTGAATT+TGG | + | chr4.2:40098531-40098550 | MS.gene056901:intron | 40.0% | |
| GGAGATGGATGCATTAATTG+CGG | + | chr4.2:40098745-40098764 | MS.gene056901:CDS | 40.0% | |
| TGAATTGATTGCAGCCGAAT+TGG | + | chr4.2:40098625-40098644 | MS.gene056901:CDS | 40.0% | |
| TGCATTAATTGCGGAACAAC+CGG | + | chr4.2:40098754-40098773 | MS.gene056901:CDS | 40.0% | |
| TGTACATGGATGGACAAGAA+AGG | + | chr4.2:40098957-40098976 | MS.gene056901:CDS | 40.0% | |
| TGTATGTGGAGAAGTCTAGT+GGG | + | chr4.2:40098990-40099009 | MS.gene056901:CDS | 40.0% | |
| TTGTATGTGGAGAAGTCTAG+TGG | + | chr4.2:40098989-40099008 | MS.gene056901:CDS | 40.0% | |
| ! | CGTAAACCTCGTTCTCTTTT+AGG | - | chr4.2:40098709-40098728 | None:intergenic | 40.0% |
| ! | CTAGTGGGTAAGTTATACAC+TGG | + | chr4.2:40099005-40099024 | MS.gene056901:intron | 40.0% |
| !! | AAGGTATGCTGCTTTGTATG+TGG | + | chr4.2:40098976-40098995 | MS.gene056901:CDS | 40.0% |
| CAACATTTCCGAGAAGTCCA+TGG | + | chr4.2:40098376-40098395 | MS.gene056901:CDS | 45.0% | |
| GGAAGAGGATATGTCTCTTC+AGG | + | chr4.2:40098646-40098665 | MS.gene056901:CDS | 45.0% | |
| GTACATGAAAGCGAGGAAAG+AGG | + | chr4.2:40098817-40098836 | MS.gene056901:CDS | 45.0% | |
| TTCCGAGAAGTCCATGGAAA+AGG | + | chr4.2:40098382-40098401 | MS.gene056901:CDS | 45.0% | |
| ! | CACCTTTTCCATGGACTTCT+CGG | - | chr4.2:40098387-40098406 | None:intergenic | 45.0% |
| ! | TGTATGTCGCACCTTTTCCA+TGG | - | chr4.2:40098396-40098415 | None:intergenic | 45.0% |
| AATGGACCTGCAAGATGCTC+CGG | - | chr4.2:40098776-40098795 | None:intergenic | 50.0% | |
| GATTGCAGCCGAATTGGAAG+AGG | + | chr4.2:40098631-40098650 | MS.gene056901:CDS | 50.0% | |
| ! | CTTGCTGTGGGCAAAACTGA+AGG | + | chr4.2:40098477-40098496 | MS.gene056901:intron | 50.0% |
| ! | GAACAACCGGAGCATCTTGC+AGG | + | chr4.2:40098767-40098786 | MS.gene056901:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 40098368 | 40099134 | 40098368 | ID=MS.gene056901 |
| chr4.2 | mRNA | 40098368 | 40099134 | 40098368 | ID=MS.gene056901.t1;Parent=MS.gene056901 |
| chr4.2 | exon | 40098368 | 40098403 | 40098368 | ID=MS.gene056901.t1.exon1;Parent=MS.gene056901.t1 |
| chr4.2 | CDS | 40098368 | 40098403 | 40098368 | ID=cds.MS.gene056901.t1;Parent=MS.gene056901.t1 |
| chr4.2 | exon | 40098617 | 40099011 | 40098617 | ID=MS.gene056901.t1.exon2;Parent=MS.gene056901.t1 |
| chr4.2 | CDS | 40098617 | 40099011 | 40098617 | ID=cds.MS.gene056901.t1;Parent=MS.gene056901.t1 |
| chr4.2 | exon | 40099092 | 40099134 | 40099092 | ID=MS.gene056901.t1.exon3;Parent=MS.gene056901.t1 |
| chr4.2 | CDS | 40099092 | 40099134 | 40099092 | ID=cds.MS.gene056901.t1;Parent=MS.gene056901.t1 |
| Gene Sequence |
| Protein sequence |
