Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96503.t1 | RHN79299.1 | 38 | 221 | 87 | 6 | 1 | 186 | 1 | 206 | 9.80E-22 | 113.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96503.t1 | A0A396JLZ9 | 38.0 | 221 | 87 | 6 | 1 | 186 | 1 | 206 | 7.0e-22 | 113.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049563 | MS.gene96503 | 0.80134 | 9.43E-49 | -1.69E-46 |
| MS.gene050139 | MS.gene96503 | 0.844364 | 7.91E-59 | -1.69E-46 |
| MS.gene050780 | MS.gene96503 | 0.80635 | 8.58E-50 | -1.69E-46 |
| MS.gene051626 | MS.gene96503 | 0.834096 | 3.65E-56 | -1.69E-46 |
| MS.gene051789 | MS.gene96503 | 0.821021 | 5.04E-53 | -1.69E-46 |
| MS.gene052187 | MS.gene96503 | 0.814423 | 1.55E-51 | -1.69E-46 |
| MS.gene052698 | MS.gene96503 | 0.841293 | 5.19E-58 | -1.69E-46 |
| MS.gene052967 | MS.gene96503 | 0.817199 | 3.73E-52 | -1.69E-46 |
| MS.gene053074 | MS.gene96503 | 0.87877 | 2.15E-69 | -1.69E-46 |
| MS.gene053184 | MS.gene96503 | 0.822571 | 2.21E-53 | -1.69E-46 |
| MS.gene053204 | MS.gene96503 | 0.807007 | 6.24E-50 | -1.69E-46 |
| MS.gene054184 | MS.gene96503 | 0.827658 | 1.39E-54 | -1.69E-46 |
| MS.gene054185 | MS.gene96503 | 0.832624 | 8.50E-56 | -1.69E-46 |
| MS.gene05456 | MS.gene96503 | 0.806638 | 7.46E-50 | -1.69E-46 |
| MS.gene054596 | MS.gene96503 | 0.845554 | 3.78E-59 | -1.69E-46 |
| MS.gene055357 | MS.gene96503 | 0.81 | 1.43E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene96503 | 0.816276 | 6.01E-52 | -1.69E-46 |
| MS.gene055359 | MS.gene96503 | 0.844263 | 8.42E-59 | -1.69E-46 |
| MS.gene057395 | MS.gene96503 | 0.815036 | 1.14E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene96503 | 0.861969 | 7.06E-64 | -1.69E-46 |
| MS.gene059006 | MS.gene96503 | 0.85141 | 9.04E-61 | -1.69E-46 |
| MS.gene059124 | MS.gene96503 | 0.809815 | 1.57E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96503.t1 | MTR_1g054385 | 46.316 | 190 | 92 | 1 | 6 | 185 | 61 | 250 | 2.97e-46 | 152 |
| MS.gene96503.t1 | MTR_1g054380 | 40.196 | 204 | 72 | 6 | 1 | 169 | 1 | 189 | 8.22e-31 | 112 |
| MS.gene96503.t1 | MTR_5g048290 | 33.333 | 201 | 96 | 5 | 21 | 186 | 85 | 282 | 1.78e-18 | 80.9 |
| MS.gene96503.t1 | MTR_1g054370 | 31.250 | 208 | 94 | 5 | 21 | 186 | 90 | 290 | 9.60e-16 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAACTTGTTCAAGGTTTA+AGG | 0.133169 | 1.4:+44178643 | None:intergenic |
| CTTGTAAACAATTCCTTCTT+TGG | 0.221022 | 1.4:+44178785 | None:intergenic |
| CTTCGAAAAGAGTGGATTAA+TGG | 0.276341 | 1.4:-44178862 | MS.gene96503:CDS |
| GGAGCAAAAGGTTTGTGATT+TGG | 0.304158 | 1.4:-44178991 | MS.gene96503:CDS |
| AAGTTGGAGGCTGAAATTAA+AGG | 0.305610 | 1.4:-44178765 | MS.gene96503:CDS |
| CTTGTTCAAGGTTTAAGGTT+AGG | 0.370602 | 1.4:+44178648 | None:intergenic |
| ATGGCTACTCTACATCAATC+TGG | 0.390287 | 1.4:-44179130 | MS.gene96503:CDS |
| GCAAGCAACACCCGTTCAAT+TGG | 0.411385 | 1.4:-44179033 | MS.gene96503:CDS |
| AGTTGGAGGCTGAAATTAAA+GGG | 0.412362 | 1.4:-44178764 | MS.gene96503:CDS |
| TGCTCGTTGTATGGAGCAAA+AGG | 0.418843 | 1.4:-44179003 | MS.gene96503:CDS |
| GGAAAATTGCACACCAAAGA+AGG | 0.418990 | 1.4:-44178798 | MS.gene96503:CDS |
| GGTCTCGATTGCTCGTTGTA+TGG | 0.434380 | 1.4:-44179012 | MS.gene96503:CDS |
| CACTAAAATTGAAGAATTGA+AGG | 0.435268 | 1.4:-44178736 | MS.gene96503:CDS |
| AGAAGGAATTGTTTACAAGT+TGG | 0.457133 | 1.4:-44178781 | MS.gene96503:CDS |
| GTGATTTGGAAATGAGCTGC+AGG | 0.481810 | 1.4:-44178977 | MS.gene96503:CDS |
| AGGAATTGTTTACAAGTTGG+AGG | 0.487776 | 1.4:-44178778 | MS.gene96503:CDS |
| AAAGGTTCATGAGGCATATG+AGG | 0.490646 | 1.4:-44179087 | MS.gene96503:CDS |
| ATTGTAGTTCAGCACAATGT+TGG | 0.502018 | 1.4:-44178708 | MS.gene96503:CDS |
| CTGAATCTGAGAATAACATA+GGG | 0.510118 | 1.4:-44178914 | MS.gene96503:CDS |
| TCTGAATCTGAGAATAACAT+AGG | 0.510558 | 1.4:-44178915 | MS.gene96503:CDS |
| GAGTGGATTAATGGAAAGAA+CGG | 0.524492 | 1.4:-44178853 | MS.gene96503:CDS |
| GCACAATGTTGGCTTTAATG+AGG | 0.525326 | 1.4:-44178697 | MS.gene96503:CDS |
| TCATGAGGCATATGAGGAGT+GGG | 0.527499 | 1.4:-44179081 | MS.gene96503:CDS |
| GCAATCGAGACCAATTGAAC+GGG | 0.527802 | 1.4:+44179023 | None:intergenic |
| GTAGAGTAGCCATTCTCAGC+TGG | 0.533250 | 1.4:+44179140 | None:intergenic |
| AAGACTACACCAGCTGAGAA+TGG | 0.552919 | 1.4:-44179149 | MS.gene96503:CDS |
| TGGCTACTCTACATCAATCT+GGG | 0.557094 | 1.4:-44179129 | MS.gene96503:CDS |
| GAGTTAATCTTCGAAAAGAG+TGG | 0.570774 | 1.4:-44178870 | MS.gene96503:CDS |
| AGAATGGATCCCAAGAGATG+TGG | 0.577195 | 1.4:-44179194 | MS.gene96503:CDS |
| TATCACAGAAAAGGTTCATG+AGG | 0.579514 | 1.4:-44179096 | MS.gene96503:CDS |
| TTCATGAGGCATATGAGGAG+TGG | 0.581486 | 1.4:-44179082 | MS.gene96503:CDS |
| CGGGTGTTGCTTGCAAAACA+CGG | 0.601763 | 1.4:+44179042 | None:intergenic |
| AGCAATCGAGACCAATTGAA+CGG | 0.624783 | 1.4:+44179022 | None:intergenic |
| TGATTTGGAAATGAGCTGCA+GGG | 0.643574 | 1.4:-44178976 | MS.gene96503:CDS |
| CATGAGGCATATGAGGAGTG+GGG | 0.686751 | 1.4:-44179080 | MS.gene96503:CDS |
| TGAATCTGAGAATAACATAG+GGG | 0.713948 | 1.4:-44178913 | MS.gene96503:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CACTAAAATTGAAGAATTGA+AGG | - | chr1.4:44179108-44179127 | MS.gene96503:CDS | 25.0% |
| !! | AATGAAAACTCCAAGATTTT+AGG | - | chr1.4:44179025-44179044 | MS.gene96503:CDS | 25.0% |
| !! | TGACAATTTTATCACAGAAA+AGG | - | chr1.4:44178739-44178758 | MS.gene96503:CDS | 25.0% |
| AGAAGGAATTGTTTACAAGT+TGG | - | chr1.4:44179063-44179082 | MS.gene96503:CDS | 30.0% | |
| CTGAATCTGAGAATAACATA+GGG | - | chr1.4:44178930-44178949 | MS.gene96503:CDS | 30.0% | |
| CTTGTAAACAATTCCTTCTT+TGG | + | chr1.4:44179062-44179081 | None:intergenic | 30.0% | |
| TCTGAATCTGAGAATAACAT+AGG | - | chr1.4:44178929-44178948 | MS.gene96503:CDS | 30.0% | |
| TGAATCTGAGAATAACATAG+GGG | - | chr1.4:44178931-44178950 | MS.gene96503:CDS | 30.0% | |
| ! | GTGCAATTTTCCTAAAATCT+TGG | + | chr1.4:44179038-44179057 | None:intergenic | 30.0% |
| AAGTTGGAGGCTGAAATTAA+AGG | - | chr1.4:44179079-44179098 | MS.gene96503:CDS | 35.0% | |
| AGTTGGAGGCTGAAATTAAA+GGG | - | chr1.4:44179080-44179099 | MS.gene96503:CDS | 35.0% | |
| ATTGTAGTTCAGCACAATGT+TGG | - | chr1.4:44179136-44179155 | MS.gene96503:CDS | 35.0% | |
| GAGTTAATCTTCGAAAAGAG+TGG | - | chr1.4:44178974-44178993 | MS.gene96503:CDS | 35.0% | |
| TATCACAGAAAAGGTTCATG+AGG | - | chr1.4:44178748-44178767 | MS.gene96503:CDS | 35.0% | |
| ! | AGGAATTGTTTACAAGTTGG+AGG | - | chr1.4:44179066-44179085 | MS.gene96503:CDS | 35.0% |
| !! | CTTCGAAAAGAGTGGATTAA+TGG | - | chr1.4:44178982-44179001 | MS.gene96503:CDS | 35.0% |
| !! | GAGTGGATTAATGGAAAGAA+CGG | - | chr1.4:44178991-44179010 | MS.gene96503:CDS | 35.0% |
| AAAGGTTCATGAGGCATATG+AGG | - | chr1.4:44178757-44178776 | MS.gene96503:CDS | 40.0% | |
| AGCAATCGAGACCAATTGAA+CGG | + | chr1.4:44178825-44178844 | None:intergenic | 40.0% | |
| ATGGCTACTCTACATCAATC+TGG | - | chr1.4:44178714-44178733 | MS.gene96503:CDS | 40.0% | |
| GGAAAATTGCACACCAAAGA+AGG | - | chr1.4:44179046-44179065 | MS.gene96503:CDS | 40.0% | |
| GGAGCAAAAGGTTTGTGATT+TGG | - | chr1.4:44178853-44178872 | MS.gene96503:CDS | 40.0% | |
| TGGCTACTCTACATCAATCT+GGG | - | chr1.4:44178715-44178734 | MS.gene96503:CDS | 40.0% | |
| ! | CTACGTTTTCCACATCTCTT+GGG | + | chr1.4:44178662-44178681 | None:intergenic | 40.0% |
| ! | TGATTTGGAAATGAGCTGCA+GGG | - | chr1.4:44178868-44178887 | MS.gene96503:CDS | 40.0% |
| !! | GCACAATGTTGGCTTTAATG+AGG | - | chr1.4:44179147-44179166 | MS.gene96503:CDS | 40.0% |
| AAGACTACACCAGCTGAGAA+TGG | - | chr1.4:44178695-44178714 | MS.gene96503:CDS | 45.0% | |
| GCAATCGAGACCAATTGAAC+GGG | + | chr1.4:44178824-44178843 | None:intergenic | 45.0% | |
| TCATGAGGCATATGAGGAGT+GGG | - | chr1.4:44178763-44178782 | MS.gene96503:CDS | 45.0% | |
| TGCTCGTTGTATGGAGCAAA+AGG | - | chr1.4:44178841-44178860 | MS.gene96503:CDS | 45.0% | |
| TTCATGAGGCATATGAGGAG+TGG | - | chr1.4:44178762-44178781 | MS.gene96503:CDS | 45.0% | |
| ! | GCTACGTTTTCCACATCTCT+TGG | + | chr1.4:44178663-44178682 | None:intergenic | 45.0% |
| ! | GTGATTTGGAAATGAGCTGC+AGG | - | chr1.4:44178867-44178886 | MS.gene96503:CDS | 45.0% |
| CATGAGGCATATGAGGAGTG+GGG | - | chr1.4:44178764-44178783 | MS.gene96503:CDS | 50.0% | |
| GCAAGCAACACCCGTTCAAT+TGG | - | chr1.4:44178811-44178830 | MS.gene96503:CDS | 50.0% | |
| GGTCTCGATTGCTCGTTGTA+TGG | - | chr1.4:44178832-44178851 | MS.gene96503:CDS | 50.0% | |
| GTAGAGTAGCCATTCTCAGC+TGG | + | chr1.4:44178707-44178726 | None:intergenic | 50.0% | |
| !! | CGGGTGTTGCTTGCAAAACA+CGG | + | chr1.4:44178805-44178824 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 44178653 | 44179213 | 44178653 | ID=MS.gene96503 |
| chr1.4 | mRNA | 44178653 | 44179213 | 44178653 | ID=MS.gene96503.t1;Parent=MS.gene96503 |
| chr1.4 | exon | 44178653 | 44179213 | 44178653 | ID=MS.gene96503.t1.exon1;Parent=MS.gene96503.t1 |
| chr1.4 | CDS | 44178653 | 44179213 | 44178653 | ID=cds.MS.gene96503.t1;Parent=MS.gene96503.t1 |
| Gene Sequence |
| Protein sequence |
