AlfalfaGEDB | MsG0080048401.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048401.01

KEGG: K14709
GO: GO:0016020 GO:0030001 GO:0046873 GO:0055085
PFAM: PF02535

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048401.01.T01	XP_013450830.1	86.667	105	12	1	3	105	80	184	1.02E-57	191

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048401.01.T01	Q9FIS2	50.962	104	47	2	3	102	78	181	7.34E-27	103

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048401.01.T01	B7FK77	86.667	105	12	1	3	105	80	184	4.87e-58	191

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048401.01	MsG0080048909.01	0.811175	7.984367e-51	4.908164e-48
MsG0080048401.01	MsG0080048969.01	0.814421	1.553900e-51	1.042221e-48
MsG0080048401.01	MsG0180003274.01	0.801908	7.211899e-49	3.485426e-46
MsG0080048401.01	MsG0180003451.01	0.831645	1.484028e-55	1.620389e-52
MsG0080048401.01	MsG0180004458.01	0.807148	5.823303e-50	3.219041e-47
MsG0080048401.01	MsG0180005991.01	0.814029	1.896786e-51	1.258710e-48
MsG0080048401.01	MsG0280007600.01	0.801650	8.146692e-49	3.911327e-46
MsG0080048401.01	MsG0280008233.01	0.811928	5.478587e-51	3.435357e-48
MsG0080048401.01	MsG0280010523.01	0.819664	1.031199e-52	7.985515e-50
MsG0080048401.01	MsG0380015636.01	0.832922	7.169472e-56	8.129641e-53
MsG0080048401.01	MsG0380016151.01	0.802337	5.885900e-49	2.875866e-46
MsG0080048401.01	MsG0380016344.01	0.802489	5.476663e-49	2.686131e-46
MsG0080048401.01	MsG0380017780.01	-0.802764	4.804650e-49	2.373309e-46
MsG0080048401.01	MsG0480019604.01	0.819144	1.355257e-52	1.034361e-49
MsG0080048401.01	MsG0480019693.01	0.823089	1.670702e-53	1.424535e-50
MsG0080048401.01	MsG0480022968.01	-0.807593	4.686328e-50	2.620591e-47
MsG0080048401.01	MsG0580024763.01	0.801367	9.309405e-49	4.437979e-46
MsG0080048401.01	MsG0580024768.01	0.800432	1.445504e-48	6.729726e-46
MsG0080048401.01	MsG0580025215.01	0.808015	3.810893e-50	2.154843e-47
MsG0080048401.01	MsG0580028092.01	0.853279	2.654522e-61	5.705290e-58
MsG0080048401.01	MsG0580028093.01	0.814639	1.390860e-51	9.383459e-49
MsG0080048401.01	MsG0580029719.01	0.809958	1.462811e-50	8.707683e-48
MsG0080048401.01	MsG0680032000.01	0.806065	9.856499e-50	5.299336e-47
MsG0080048401.01	MsG0680032002.01	0.811675	6.219307e-51	3.873567e-48
MsG0080048401.01	MsG0680035612.01	0.807485	4.940674e-50	2.755263e-47
MsG0080048401.01	MsG0780036584.01	0.804928	1.706895e-49	8.913480e-47
MsG0080048401.01	MsG0780037963.01	0.805492	1.300338e-49	6.889065e-47
MsG0080048401.01	MsG0780040080.01	0.805346	1.395608e-49	7.366019e-47
MsG0080048401.01	MsG0780040319.01	0.813716	2.222958e-51	1.462745e-48
MsG0080048401.01	MsG0880042006.01	0.826338	2.865215e-54	2.679504e-51
MsG0080048401.01	MsG0880042343.01	0.800559	1.361717e-48	6.359971e-46
MsG0080048401.01	MsG0880044636.01	0.805121	1.555753e-49	8.164099e-47
MsG0080048401.01	MsG0880045261.01	0.811205	7.865355e-51	4.838859e-48
MsG0080048401.01	MsG0880045377.01	0.811766	5.940675e-51	3.709239e-48
MsG0080048401.01	MsG0880045409.01	0.802047	6.752849e-49	3.275055e-46
MsG0080048401.01	MsG0880046406.01	0.810805	9.602867e-51	5.845472e-48
MsG0080048401.01	MsG0880046968.01	0.815173	1.058853e-51	7.246816e-49
MsG0080048401.01	MsG0880046969.01	0.800402	1.466014e-48	6.819965e-46
MsG0080048401.01	MsG0880047161.01	0.816303	5.928976e-52	4.185885e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048401.01.T01	MTR_6g007687	86.667	105	12	1	3	105	80	184	1.24e-61	191
MsG0080048401.01.T01	MTR_3g081690	52.874	87	41	0	5	91	77	163	5.37e-27	101
MsG0080048401.01.T01	MTR_3g081690	52.874	87	41	0	5	91	100	186	6.18e-27	101
MsG0080048401.01.T01	MTR_3g082050	52.874	87	41	0	5	91	77	163	6.74e-27	101
MsG0080048401.01.T01	MTR_3g082050	52.874	87	41	0	5	91	100	186	6.92e-27	101
MsG0080048401.01.T01	MTR_2g064310	53.086	81	38	0	5	85	84	164	7.65e-27	101
MsG0080048401.01.T01	MTR_3g082050	52.874	87	41	0	5	91	105	191	7.72e-27	101
MsG0080048401.01.T01	MTR_3g081640	55.128	78	35	0	5	82	81	158	7.00e-26	99.0
MsG0080048401.01.T01	MTR_3g081580	56.250	80	35	0	5	84	81	160	1.10e-24	95.5
MsG0080048401.01.T01	MTR_3g104400	48.837	86	43	1	8	93	62	146	3.61e-21	86.3
MsG0080048401.01.T01	MTR_4g083570	44.186	86	44	1	6	91	83	164	3.51e-19	80.9
MsG0080048401.01.T01	MTR_8g105030	49.206	63	32	0	6	68	84	146	4.27e-19	80.9
MsG0080048401.01.T01	MTR_3g081630	65.517	58	19	1	4	61	22	78	2.34e-18	74.7
MsG0080048401.01.T01	MTR_1g016120	45.714	70	36	1	8	75	70	139	1.21e-13	65.5
MsG0080048401.01.T01	MTR_5g071990	41.463	82	44	3	4	84	60	138	1.37e-12	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048401.01.T01	AT5G62160	50.962	104	47	2	3	102	78	181	7.48e-28	103
MsG0080048401.01.T01	AT3G12750	42.857	112	51	2	5	103	86	197	1.01e-24	95.9
MsG0080048401.01.T01	AT1G05300	49.398	83	41	1	3	85	82	163	2.07e-22	88.2
MsG0080048401.01.T01	AT2G04032	40.196	102	51	1	6	97	92	193	3.34e-22	89.0
MsG0080048401.01.T01	AT1G05300	49.398	83	41	1	3	85	82	163	6.81e-22	88.2
MsG0080048401.01.T01	AT2G32270	45.098	102	54	2	3	104	87	186	1.85e-21	86.7
MsG0080048401.01.T01	AT1G31260	41.489	94	53	1	5	98	87	178	7.71e-21	85.5
MsG0080048401.01.T01	AT4G33020	50.000	82	41	0	8	89	32	113	3.94e-19	80.5
MsG0080048401.01.T01	AT4G33020	55.882	68	30	0	8	75	79	146	3.95e-19	80.9
MsG0080048401.01.T01	AT4G19690	36.449	107	60	2	6	104	88	194	2.12e-18	76.6
MsG0080048401.01.T01	AT1G10970	49.333	75	37	1	8	82	66	139	2.37e-18	78.6
MsG0080048401.01.T01	AT1G10970	49.333	75	37	1	8	82	66	139	2.37e-18	78.6
MsG0080048401.01.T01	AT4G19680	35.052	97	58	1	6	97	83	179	1.25e-17	75.5
MsG0080048401.01.T01	AT4G19690	36.449	107	60	2	6	104	88	194	1.42e-17	76.3
MsG0080048401.01.T01	AT1G60960	47.887	71	37	0	8	78	102	172	1.63e-17	75.9
MsG0080048401.01.T01	AT4G19680	35.052	97	58	1	6	97	83	179	3.87e-17	75.1
MsG0080048401.01.T01	AT1G60960	47.887	71	37	0	8	78	92	162	4.75e-17	75.1
MsG0080048401.01.T01	AT1G60960	47.887	71	37	0	8	78	102	172	5.29e-17	75.1
MsG0080048401.01.T01	AT2G30080	48.333	60	30	1	4	62	62	121	2.84e-13	64.3

Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTATGGAAGCTTTGATTA+TGG	0.209670	contig302end:-33161	MsG0080048401.01.T01:CDS
ATTATGGAAGCTTTGATTAT+GGG	0.290259	contig302end:-33160	MsG0080048401.01.T01:CDS
AGCCATTGGAACATTAATTA+TGG	0.327032	contig302end:-33176	MsG0080048401.01.T01:CDS
GAGACTCATCATTCAGATAA+TGG	0.382242	contig302end:-33085	MsG0080048401.01.T01:CDS
CATACTTCCTGATGCTTTCG+AGG	0.388835	contig302end:-33272	MsG0080048401.01.T01:CDS
TGGGGAATGTTTCCTTTCTC+CGG	0.432176	contig302end:-33217	MsG0080048401.01.T01:CDS
GTAACAATGGTTGCAGCCAT+TGG	0.447857	contig302end:-33190	MsG0080048401.01.T01:CDS
TTGGTTAAGGCGTTTGCTGC+TGG	0.463059	contig302end:-33322	MsG0080048401.01.T01:CDS
TGCAACCATTGTTACAAATC+CGG	0.470412	contig302end:+33198	None:intergenic
GTTAACGCCTCGAAAGCATC+AGG	0.499868	contig302end:+33265	None:intergenic
GCTGGTGTGATCCTAGCGAC+CGG	0.504781	contig302end:-33304	MsG0080048401.01.T01:CDS
ATTGTTACAAATCCGGAGAA+AGG	0.516006	contig302end:+33205	None:intergenic
GCGTTAACGAGTCCTTGTAT+AGG	0.524663	contig302end:-33250	MsG0080048401.01.T01:CDS
TTCCATAATTAATGTTCCAA+TGG	0.527906	contig302end:+33174	None:intergenic
TGTGTATGAACCCGGTCGCT+AGG	0.531948	contig302end:+33293	None:intergenic
CTTGTATAGGCGAAAAGCCA+TGG	0.536617	contig302end:-33237	MsG0080048401.01.T01:CDS
CTGGTGTGATCCTAGCGACC+GGG	0.540501	contig302end:-33303	MsG0080048401.01.T01:CDS
TTGTATAGGCGAAAAGCCAT+GGG	0.547614	contig302end:-33236	MsG0080048401.01.T01:CDS
GTCTCATCATTCTCATCCAA+TGG	0.548232	contig302end:+33103	None:intergenic
TTTCTCCGGATTTGTAACAA+TGG	0.590253	contig302end:-33203	MsG0080048401.01.T01:CDS
AGGAAGTATGTGTATGAACC+CGG	0.632124	contig302end:+33285	None:intergenic
GAGAAAGGAAACATTCCCCA+TGG	0.669527	contig302end:+33220	None:intergenic
TGTATAGGCGAAAAGCCATG+GGG	0.764297	contig302end:-33235	MsG0080048401.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GATTTTTATTTTTTTGGTTA+AGG	-	contig302end:33044-33063	MsG0080048401.01.T01:CDS	15.0%
!!!	TTTTCATTTCAGATCTTTTA+TGG	+	contig302end:33248-33267	None:intergenic	20.0%
!	TTCCATAATTAATGTTCCAA+TGG	+	contig302end:33208-33227	None:intergenic	25.0%
!!	AATTATGGAAGCTTTGATTA+TGG	-	contig302end:33218-33237	MsG0080048401.01.T01:CDS	25.0%
!!	ATTATGGAAGCTTTGATTAT+GGG	-	contig302end:33219-33238	MsG0080048401.01.T01:CDS	25.0%
!!!	CTCAAATTTTTCAATTGCAT+CGG	-	contig302end:33328-33347	MsG0080048401.01.T01:CDS	25.0%
	AATGAAAAAATCTCAGCCAT+TGG	-	contig302end:33260-33279	MsG0080048401.01.T01:CDS	30.0%
	AGCCATTGGAACATTAATTA+TGG	-	contig302end:33203-33222	MsG0080048401.01.T01:CDS	30.0%
	ATTGTTACAAATCCGGAGAA+AGG	+	contig302end:33177-33196	None:intergenic	35.0%
	GAGACTCATCATTCAGATAA+TGG	-	contig302end:33294-33313	MsG0080048401.01.T01:CDS	35.0%
	TGCAACCATTGTTACAAATC+CGG	+	contig302end:33184-33203	None:intergenic	35.0%
	TTTCTCCGGATTTGTAACAA+TGG	-	contig302end:33176-33195	MsG0080048401.01.T01:CDS	35.0%
	GTCTCATCATTCTCATCCAA+TGG	+	contig302end:33279-33298	None:intergenic	40.0%
	TTGTATAGGCGAAAAGCCAT+GGG	-	contig302end:33143-33162	MsG0080048401.01.T01:CDS	40.0%
!	AGGAAGTATGTGTATGAACC+CGG	+	contig302end:33097-33116	None:intergenic	40.0%
	CATACTTCCTGATGCTTTCG+AGG	-	contig302end:33107-33126	MsG0080048401.01.T01:CDS	45.0%
	CTTGTATAGGCGAAAAGCCA+TGG	-	contig302end:33142-33161	MsG0080048401.01.T01:CDS	45.0%
	GAGAAAGGAAACATTCCCCA+TGG	+	contig302end:33162-33181	None:intergenic	45.0%
	GCGTTAACGAGTCCTTGTAT+AGG	-	contig302end:33129-33148	MsG0080048401.01.T01:CDS	45.0%
	GTAACAATGGTTGCAGCCAT+TGG	-	contig302end:33189-33208	MsG0080048401.01.T01:CDS	45.0%
	TGGGGAATGTTTCCTTTCTC+CGG	-	contig302end:33162-33181	MsG0080048401.01.T01:CDS	45.0%
	TGTATAGGCGAAAAGCCATG+GGG	-	contig302end:33144-33163	MsG0080048401.01.T01:CDS	45.0%
!	CATGGCTTTTCGCCTATACA+AGG	+	contig302end:33144-33163	None:intergenic	45.0%
!	TTGGTTAAGGCGTTTGCTGC+TGG	-	contig302end:33057-33076	MsG0080048401.01.T01:CDS	50.0%
!!	GTTAACGCCTCGAAAGCATC+AGG	+	contig302end:33117-33136	None:intergenic	50.0%
	TGTGTATGAACCCGGTCGCT+AGG	+	contig302end:33089-33108	None:intergenic	55.0%
	CTGGTGTGATCCTAGCGACC+GGG	-	contig302end:33076-33095	MsG0080048401.01.T01:CDS	60.0%
	GCTGGTGTGATCCTAGCGAC+CGG	-	contig302end:33075-33094	MsG0080048401.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
contig302end	gene	33042	33359	33042	ID=MsG0080048401.01;Name=MsG0080048401.01
contig302end	mRNA	33042	33359	33042	ID=MsG0080048401.01.T01;Parent=MsG0080048401.01;Name=MsG0080048401.01.T01;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|105
contig302end	exon	33042	33359	33042	ID=MsG0080048401.01.T01:exon:3627;Parent=MsG0080048401.01.T01
contig302end	CDS	33042	33359	33042	ID=MsG0080048401.01.T01:cds;Parent=MsG0080048401.01.T01

Gene Sequence	>MsG0080048401.01.T01 ATGATTTTTATTTTTTTGGTTAAGGCGTTTGCTGCTGGTGTGATCCTAGCGACCGGGTTCATACACATACTTCCTGATGCTTTCGAGGCGTTAACGAGTCCTTGTATAGGCGAAAAGCCATGGGGAATGTTTCCTTTCTCCGGATTTGTAACAATGGTTGCAGCCATTGGAACATTAATTATGGAAGCTTTGATTATGGGCTACCATAAAAGATCTGAAATGAAAAAATCTCAGCCATTGGATGAGAATGATGAGACTCATCATTCAGATAATGGTAGTAGTCATGCTCAAATTTTTCAATTGCATCGGATAGATTAG
Protein sequence	>MsG0080048401.01.T01 MIFIFLVKAFAAGVILATGFIHILPDAFEALTSPCIGEKPWGMFPFSGFVTMVAAIGTLIMEALIMGYHKRSEMKKSQPLDENDETHHSDNGSSHAQIFQLHRID*