AlfalfaGEDB | MsG0480021252.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021252.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021252.01.T01	XP_003606627.1	99.324	148	1	0	1	148	1	148	3.31E-98	289

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021252.01.T01	Q1S9I9	99.324	148	1	0	1	148	1	148	8.77E-101	288

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021252.01.T01	G7JTQ7	99.324	148	1	0	1	148	1	148	1.58e-98	289

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004995.01	MsG0480021252.01	0.855166	7.570938e-62	1.731988e-58
MsG0180005048.01	MsG0480021252.01	0.858607	7.333091e-63	1.882251e-59
MsG0180006133.01	MsG0480021252.01	0.833701	4.585858e-56	5.322457e-53
MsG0480020918.01	MsG0480021252.01	0.847127	1.407418e-59	2.477065e-56
MsG0480020927.01	MsG0480021252.01	0.825204	5.325515e-54	4.822028e-51
MsG0480020971.01	MsG0480021252.01	0.862403	5.194632e-64	1.519421e-60
MsG0480021015.01	MsG0480021252.01	0.827922	1.197257e-54	1.172213e-51
MsG0480021252.01	MsG0480021301.01	0.809347	1.978996e-50	1.159162e-47
MsG0480021252.01	MsG0480021302.01	0.830963	2.183732e-55	2.336587e-52
MsG0480021252.01	MsG0480022982.01	0.828893	6.979522e-55	7.029085e-52
MsG0480021252.01	MsG0580025177.01	0.845606	3.656338e-59	6.126104e-56
MsG0480021252.01	MsG0580025506.01	0.832650	8.377213e-56	9.422776e-53
MsG0480021252.01	MsG0780040620.01	0.823335	1.463980e-53	1.256821e-50
MsG0480021252.01	MsG0780040844.01	0.805283	1.438602e-49	7.580409e-47
MsG0480021252.01	MsG0780041143.01	0.801406	9.141640e-49	4.362138e-46
MsG0380015058.01	MsG0480021252.01	0.814725	1.330940e-51	9.000074e-49
MsG0380015234.01	MsG0480021252.01	0.829083	6.277395e-55	6.357714e-52

PPI

Gene1	Gene2	Type
MsG0180000243.01	MsG0480021252.01	PPI
MsG0580025506.01	MsG0480021252.01	PPI
MsG0480021252.01	MsG0580029194.01	PPI
MsG0880044910.01	MsG0480021252.01	PPI
MsG0480021252.01	MsG0480022307.01	PPI
MsG0480021252.01	MsG0880044910.01	PPI
MsG0580027231.01	MsG0480021252.01	PPI
MsG0880047260.01	MsG0480021252.01	PPI
MsG0480021252.01	MsG0880042844.01	PPI
MsG0780038642.01	MsG0480021252.01	PPI
MsG0480021252.01	MsG0380012788.01	PPI
MsG0480021252.01	MsG0280008380.01	PPI
MsG0480020877.01	MsG0480021252.01	PPI
MsG0480021252.01	MsG0480023842.01	PPI
MsG0480021252.01	MsG0780041592.01	PPI
MsG0480021252.01	MsG0580025504.01	PPI
MsG0480021252.01	MsG0780036534.01	PPI
MsG0480021252.01	MsG0280007919.01	PPI
MsG0880045015.01	MsG0480021252.01	PPI
MsG0480021252.01	MsG0780041593.01	PPI
MsG0580029044.01	MsG0480021252.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021252.01.T01	MTR_4g063220	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480021252.01.T01	MTR_4g063990	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480021252.01.T01	MTR_4g063200	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480021252.01.T01	MTR_4g064020	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480021252.01.T01	MTR_4g071180	99.324	148	1	0	1	148	1	148	8.54e-102	288
MsG0480021252.01.T01	MTR_4g070240	99.324	148	1	0	1	148	1	148	8.54e-102	288
MsG0480021252.01.T01	MTR_4g070245	97.973	148	3	0	1	148	1	148	4.20e-100	284
MsG0480021252.01.T01	MTR_4g063240	98.649	148	1	1	1	148	1	147	1.15e-99	283
MsG0480021252.01.T01	MTR_4g063275	96.622	148	5	0	1	148	1	148	8.49e-99	280
MsG0480021252.01.T01	MTR_4g064983	98.611	144	2	0	1	144	1	144	8.23e-97	279
MsG0480021252.01.T01	MTR_4g088025	93.919	148	4	1	1	148	1	143	8.30e-89	255
MsG0480021252.01.T01	MTR_1g090780	88.514	148	6	3	1	148	1	137	1.68e-78	229
MsG0480021252.01.T01	MTR_1g492910	87.838	148	7	3	1	148	1	137	8.34e-78	227
MsG0480021252.01.T01	MTR_1g068600	92.793	111	4	1	38	148	30	136	3.40e-64	192
MsG0480021252.01.T01	MTR_7g099960	92.793	111	5	1	38	148	31	138	6.27e-64	192
MsG0480021252.01.T01	MTR_2g084480	61.798	89	34	0	59	147	136	224	4.83e-37	127
MsG0480021252.01.T01	MTR_8g069990	68.817	93	21	3	15	107	4	88	3.55e-34	115
MsG0480021252.01.T01	MTR_4g063270	90.196	51	5	0	86	136	1	51	5.24e-27	95.9
MsG0480021252.01.T01	MTR_7g114580	90.244	41	4	0	108	148	11	51	9.46e-20	77.4
MsG0480021252.01.T01	MTR_4g032777	36.792	106	63	2	31	132	5	110	1.73e-13	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021252.01.T01	AT3G45980	88.742	151	13	2	1	148	1	150	1.47e-83	242
MsG0480021252.01.T01	AT2G28720	86.093	151	18	1	1	148	1	151	9.69e-81	235
MsG0480021252.01.T01	AT1G07790	86.928	153	10	3	1	148	1	148	7.69e-80	233
MsG0480021252.01.T01	AT5G59910	91.176	136	9	2	13	148	18	150	2.65e-77	226
MsG0480021252.01.T01	AT3G46030	94.444	126	6	1	23	148	21	145	8.66e-76	222
MsG0480021252.01.T01	AT2G37470	82.432	148	15	3	1	147	1	138	6.01e-71	210
MsG0480021252.01.T01	AT3G53650	81.208	149	16	3	1	148	1	138	4.61e-69	205
MsG0480021252.01.T01	AT5G22880	87.586	145	16	2	4	148	3	145	4.25e-67	200
MsG0480021252.01.T01	AT5G02570	79.730	148	14	1	1	148	1	132	9.71e-67	199
MsG0480021252.01.T01	AT3G09480	84.127	126	16	1	23	148	5	126	2.13e-62	187
MsG0480021252.01.T01	AT1G08170	61.176	85	33	0	61	145	151	235	2.97e-37	127

Find 46 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCTAAGAGCTTGTGAATT+TGG	0.203095	4:-55850569	None:intergenic
GGTAGCTTCTTTCCAGCCTT+TGG	0.299167	4:-55850235	None:intergenic
GCTTGTTCTGCCCGGTGAAT+TGG	0.304089	4:+55850510	MsG0480021252.01.T01:CDS
GCTGGCTTCTTCTCTCCCTT+TGG	0.362568	4:-55850148	None:intergenic
CTTGTTGTACCTAGCGAGTC+TGG	0.384866	4:-55850446	None:intergenic
TCTGCGGGTTTCTTCTCTGC+TGG	0.399254	4:-55850166	None:intergenic
GCCTTTGGCTTCTTCTCCGC+CGG	0.402654	4:-55850220	None:intergenic
GCGGCTGAACCACCTTCCTT+TGG	0.410543	4:-55850256	None:intergenic
GTGGATTTCTTCTCCTCTGC+GGG	0.431011	4:-55850181	None:intergenic
TCTCTTCTTCTTCTTGTCTC+CGG	0.444808	4:-55850278	None:intergenic
ACCCTCAGAAACAGCATGCT+TGG	0.462043	4:-55850533	None:intergenic
TTGCTTGAGATACCAATGTC+CGG	0.465790	4:-55850361	None:intergenic
AGCATGCTTGGCCAATTCAC+CGG	0.471051	4:-55850521	None:intergenic
GGGAAATTCAGACCGCGGTC+AGG	0.471115	4:+55850488	MsG0480021252.01.T01:CDS
TGTGAATTTGGTCACAGCCT+TGG	0.493306	4:-55850557	None:intergenic
AATTCCTTCAATGGCGCCAA+AGG	0.495983	4:+55850132	None:intergenic
GCGGAGAAGAAGCCAAAGGC+TGG	0.504484	4:+55850223	MsG0480021252.01.T01:CDS
AAGGAAGGTGGTTCAGCCGC+CGG	0.504946	4:+55850259	MsG0480021252.01.T01:CDS
TGCTTGAGATACCAATGTCC+GGG	0.518516	4:-55850360	None:intergenic
GCATGCTTGGCCAATTCACC+GGG	0.525255	4:-55850520	None:intergenic
TGGTATCTCAAGCAAAGCCA+TGG	0.533917	4:+55850369	MsG0480021252.01.T01:CDS
GCCAAGCATGCTGTTTCTGA+GGG	0.538001	4:+55850532	MsG0480021252.01.T01:CDS
GGCCAAGCATGCTGTTTCTG+AGG	0.538906	4:+55850531	MsG0480021252.01.T01:CDS
CACAGTCGCTGAGAAAGCTC+CGG	0.552253	4:+55850201	MsG0480021252.01.T01:CDS
GCGGTCAGGCTTGTTCTGCC+CGG	0.555879	4:+55850502	MsG0480021252.01.T01:CDS
ATTCCTTCAATGGCGCCAAA+GGG	0.555903	4:+55850133	None:intergenic
ATTTCCCTCGAAGTGATCGT+CGG	0.569915	4:-55850472	None:intergenic
TGCTGTTTCTGAGGGTACCA+AGG	0.572597	4:+55850540	MsG0480021252.01.T01:CDS
CTTCTTCTTCTTGTCTCCGG+CGG	0.574610	4:-55850275	None:intergenic
AAACAAGTTCACCCGGACAT+TGG	0.574952	4:+55850349	MsG0480021252.01.T01:CDS
AGAAGCCGACGATCACTTCG+AGG	0.586465	4:+55850467	MsG0480021252.01.T01:CDS
AAGAATCTTCCAGACTCGCT+AGG	0.594905	4:+55850437	MsG0480021252.01.T01:CDS
TGTGGATTTCTTCTCCTCTG+CGG	0.602479	4:-55850182	None:intergenic
GGAAAGAAGCTACCAAAGGA+AGG	0.624050	4:+55850244	MsG0480021252.01.T01:CDS
CGGAGCTTTCTCAGCGACTG+TGG	0.628186	4:-55850200	None:intergenic
GGCTGGAAAGAAGCTACCAA+AGG	0.634160	4:+55850240	MsG0480021252.01.T01:CDS
AGTTCTGAAACAAGTTCACC+CGG	0.635033	4:+55850342	MsG0480021252.01.T01:CDS
AGTCGCTGAGAAAGCTCCGG+CGG	0.656886	4:+55850204	MsG0480021252.01.T01:CDS
GGTATCTCAAGCAAAGCCAT+GGG	0.659631	4:+55850370	MsG0480021252.01.T01:CDS
GAAGCCGACGATCACTTCGA+GGG	0.660226	4:+55850468	MsG0480021252.01.T01:CDS
AAGAAGCTACCAAAGGAAGG+TGG	0.666846	4:+55850247	MsG0480021252.01.T01:CDS
TCCGGCGGAGAAGAAGCCAA+AGG	0.682003	4:+55850219	MsG0480021252.01.T01:CDS
GAAACTGTTCATGATACCCA+TGG	0.683953	4:-55850386	None:intergenic
GGGCAGAACAAGCCTGACCG+CGG	0.695125	4:-55850500	None:intergenic
AGCAGAGAAGAAACCCGCAG+AGG	0.698236	4:+55850168	MsG0480021252.01.T01:CDS
TTCGAGGGAAATTCAGACCG+CGG	0.709650	4:+55850483	MsG0480021252.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GAAACTGTTCATGATACCCA+TGG	-	Chr4:55850389-55850408	None:intergenic	40.0%
	TCTCTTCTTCTTCTTGTCTC+CGG	-	Chr4:55850281-55850300	None:intergenic	40.0%
	TTGCTTGAGATACCAATGTC+CGG	-	Chr4:55850364-55850383	None:intergenic	40.0%
!!	AGTTCTGAAACAAGTTCACC+CGG	+	Chr4:55850342-55850361	MsG0480021252.01.T01:CDS	40.0%
	AAACAAGTTCACCCGGACAT+TGG	+	Chr4:55850349-55850368	MsG0480021252.01.T01:CDS	45.0%
	AAGAAGCTACCAAAGGAAGG+TGG	+	Chr4:55850247-55850266	MsG0480021252.01.T01:CDS	45.0%
	AAGAATCTTCCAGACTCGCT+AGG	+	Chr4:55850437-55850456	MsG0480021252.01.T01:CDS	45.0%
	ATTTCCCTCGAAGTGATCGT+CGG	-	Chr4:55850475-55850494	None:intergenic	45.0%
	GGAAAGAAGCTACCAAAGGA+AGG	+	Chr4:55850244-55850263	MsG0480021252.01.T01:CDS	45.0%
	GGTATCTCAAGCAAAGCCAT+GGG	+	Chr4:55850370-55850389	MsG0480021252.01.T01:CDS	45.0%
	TGCTTGAGATACCAATGTCC+GGG	-	Chr4:55850363-55850382	None:intergenic	45.0%
	TGGTATCTCAAGCAAAGCCA+TGG	+	Chr4:55850369-55850388	MsG0480021252.01.T01:CDS	45.0%
	TGTGAATTTGGTCACAGCCT+TGG	-	Chr4:55850560-55850579	None:intergenic	45.0%
	TGTGGATTTCTTCTCCTCTG+CGG	-	Chr4:55850185-55850204	None:intergenic	45.0%
	ACCCTCAGAAACAGCATGCT+TGG	-	Chr4:55850536-55850555	None:intergenic	50.0%
	AGCATGCTTGGCCAATTCAC+CGG	-	Chr4:55850524-55850543	None:intergenic	50.0%
	CTTCTTCTTCTTGTCTCCGG+CGG	-	Chr4:55850278-55850297	None:intergenic	50.0%
	GGCTGGAAAGAAGCTACCAA+AGG	+	Chr4:55850240-55850259	MsG0480021252.01.T01:CDS	50.0%
	GGTAGCTTCTTTCCAGCCTT+TGG	-	Chr4:55850238-55850257	None:intergenic	50.0%
	GTGGATTTCTTCTCCTCTGC+GGG	-	Chr4:55850184-55850203	None:intergenic	50.0%
	TTCGAGGGAAATTCAGACCG+CGG	+	Chr4:55850483-55850502	MsG0480021252.01.T01:CDS	50.0%
!	CTTGTTGTACCTAGCGAGTC+TGG	-	Chr4:55850449-55850468	None:intergenic	50.0%
!!	GCCAAGCATGCTGTTTCTGA+GGG	+	Chr4:55850532-55850551	MsG0480021252.01.T01:CDS	50.0%
!!	TGCTGTTTCTGAGGGTACCA+AGG	+	Chr4:55850540-55850559	MsG0480021252.01.T01:CDS	50.0%
	AGAAGCCGACGATCACTTCG+AGG	+	Chr4:55850467-55850486	MsG0480021252.01.T01:CDS	55.0%
	AGCAGAGAAGAAACCCGCAG+AGG	+	Chr4:55850168-55850187	MsG0480021252.01.T01:CDS	55.0%
	CACAGTCGCTGAGAAAGCTC+CGG	+	Chr4:55850201-55850220	MsG0480021252.01.T01:CDS	55.0%
	GAAGCCGACGATCACTTCGA+GGG	+	Chr4:55850468-55850487	MsG0480021252.01.T01:CDS	55.0%
	GCATGCTTGGCCAATTCACC+GGG	-	Chr4:55850523-55850542	None:intergenic	55.0%
	GCTTGTTCTGCCCGGTGAAT+TGG	+	Chr4:55850510-55850529	MsG0480021252.01.T01:CDS	55.0%
	TCTGCGGGTTTCTTCTCTGC+TGG	-	Chr4:55850169-55850188	None:intergenic	55.0%
!	GCTGGCTTCTTCTCTCCCTT+TGG	-	Chr4:55850151-55850170	None:intergenic	55.0%
!!	GGCCAAGCATGCTGTTTCTG+AGG	+	Chr4:55850531-55850550	MsG0480021252.01.T01:CDS	55.0%
	AAGGAAGGTGGTTCAGCCGC+CGG	+	Chr4:55850259-55850278	MsG0480021252.01.T01:CDS	60.0%
	AGTCGCTGAGAAAGCTCCGG+CGG	+	Chr4:55850204-55850223	MsG0480021252.01.T01:CDS	60.0%
	CGGAGCTTTCTCAGCGACTG+TGG	-	Chr4:55850203-55850222	None:intergenic	60.0%
	GCGGAGAAGAAGCCAAAGGC+TGG	+	Chr4:55850223-55850242	MsG0480021252.01.T01:CDS	60.0%
	GCGGCTGAACCACCTTCCTT+TGG	-	Chr4:55850259-55850278	None:intergenic	60.0%
	GGGAAATTCAGACCGCGGTC+AGG	+	Chr4:55850488-55850507	MsG0480021252.01.T01:CDS	60.0%
	TCCGGCGGAGAAGAAGCCAA+AGG	+	Chr4:55850219-55850238	MsG0480021252.01.T01:CDS	60.0%
!	GCCTTTGGCTTCTTCTCCGC+CGG	-	Chr4:55850223-55850242	None:intergenic	60.0%
	GGGCAGAACAAGCCTGACCG+CGG	-	Chr4:55850503-55850522	None:intergenic	65.0%
!	GCGGTCAGGCTTGTTCTGCC+CGG	+	Chr4:55850502-55850521	MsG0480021252.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	55850142	55850588	55850142	ID=MsG0480021252.01;Name=MsG0480021252.01
Chr4	mRNA	55850142	55850588	55850142	ID=MsG0480021252.01.T01;Parent=MsG0480021252.01;Name=MsG0480021252.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr4	exon	55850142	55850588	55850142	ID=MsG0480021252.01.T01:exon:8150;Parent=MsG0480021252.01.T01
Chr4	CDS	55850142	55850588	55850142	ID=MsG0480021252.01.T01:cds;Parent=MsG0480021252.01.T01

Gene Sequence

>MsG0480021252.01.T01

ATGGCGCCAAAGGGAGAGAAGAAGCCAGCAGAGAAGAAACCCGCAGAGGAGAAGAAATCCACAGTCGCTGAGAAAGCTCCGGCGGAGAAGAAGCCAAAGGCTGGAAAGAAGCTACCAAAGGAAGGTGGTTCAGCCGCCGGAGACAAGAAGAAGAAGAGAAGCAAGAAGAGCGTTGAAACATACAAGATTTACATCTTCAAAGTTCTGAAACAAGTTCACCCGGACATTGGTATCTCAAGCAAAGCCATGGGTATCATGAACAGTTTCATCAACGACATCTTCGAGAAGCTCGCTCAAGAATCTTCCAGACTCGCTAGGTACAACAAGAAGCCGACGATCACTTCGAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGTGAATTGGCCAAGCATGCTGTTTCTGAGGGTACCAAGGCTGTGACCAAATTCACAAGCTCTTAG

Protein sequence

>MsG0480021252.01.T01

MAPKGEKKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*