Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020927.01.T01 | XP_003606627.1 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 1.75E-97 | 287 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020927.01.T01 | Q1S9I9 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 3.94E-100 | 286 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005048.01 | MsG0480020927.01 | 0.800968 | 1.123546e-48 | 5.302638e-46 |
| MsG0380014684.01 | MsG0480020927.01 | 0.859937 | 2.926755e-63 | 7.863542e-60 |
| MsG0480020918.01 | MsG0480020927.01 | 0.855771 | 5.044990e-62 | 1.177741e-58 |
| MsG0480020920.01 | MsG0480020927.01 | 0.821006 | 5.078333e-53 | 4.082664e-50 |
| MsG0480020927.01 | MsG0480020969.01 | 0.830995 | 2.145222e-55 | 2.297550e-52 |
| MsG0480020927.01 | MsG0480020971.01 | 0.852809 | 3.618949e-61 | 7.655446e-58 |
| MsG0480020927.01 | MsG0480021015.01 | 0.866000 | 3.924790e-65 | 1.302619e-61 |
| MsG0480020927.01 | MsG0480021252.01 | 0.825204 | 5.325515e-54 | 4.822028e-51 |
| MsG0480020927.01 | MsG0480021302.01 | 0.898888 | 3.409723e-77 | 4.112013e-73 |
| MsG0480020927.01 | MsG0480022982.01 | 0.802218 | 6.226804e-49 | 3.033083e-46 |
| MsG0480020927.01 | MsG0780040620.01 | 0.802210 | 6.250567e-49 | 3.044057e-46 |
| MsG0480020927.01 | MsG0780040844.01 | 0.819011 | 1.452822e-52 | 1.104943e-49 |
| MsG0480020927.01 | MsG0780041592.01 | 0.846453 | 2.151183e-59 | 3.704905e-56 |
| MsG0380015234.01 | MsG0480020927.01 | 0.846062 | 2.750295e-59 | 4.676606e-56 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020927.01.T01 | MTR_4g063220 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 2.12e-101 | 287 |
| MsG0480020927.01.T01 | MTR_4g063990 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 2.12e-101 | 287 |
| MsG0480020927.01.T01 | MTR_4g063200 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 2.12e-101 | 287 |
| MsG0480020927.01.T01 | MTR_4g064020 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 2.12e-101 | 287 |
| MsG0480020927.01.T01 | MTR_4g071180 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 3.84e-101 | 286 |
| MsG0480020927.01.T01 | MTR_4g070240 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 3.84e-101 | 286 |
| MsG0480020927.01.T01 | MTR_4g070245 | 97.297 | 148 | 4 | 0 | 1 | 148 | 1 | 148 | 1.17e-99 | 283 |
| MsG0480020927.01.T01 | MTR_4g063240 | 97.973 | 148 | 2 | 1 | 1 | 148 | 1 | 147 | 3.77e-99 | 281 |
| MsG0480020927.01.T01 | MTR_4g064983 | 97.917 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 3.65e-96 | 277 |
| MsG0480020927.01.T01 | MTR_4g088025 | 94.595 | 148 | 3 | 1 | 1 | 148 | 1 | 143 | 3.38e-95 | 271 |
| MsG0480020927.01.T01 | MTR_4g063275 | 95.946 | 148 | 6 | 0 | 1 | 148 | 1 | 148 | 6.96e-93 | 265 |
| MsG0480020927.01.T01 | MTR_1g090780 | 89.189 | 148 | 5 | 3 | 1 | 148 | 1 | 137 | 2.00e-78 | 228 |
| MsG0480020927.01.T01 | MTR_1g492910 | 88.514 | 148 | 6 | 3 | 1 | 148 | 1 | 137 | 9.01e-78 | 227 |
| MsG0480020927.01.T01 | MTR_1g068600 | 92.793 | 111 | 4 | 1 | 38 | 148 | 30 | 136 | 3.59e-64 | 192 |
| MsG0480020927.01.T01 | MTR_7g099960 | 92.793 | 111 | 5 | 1 | 38 | 148 | 31 | 138 | 7.31e-64 | 192 |
| MsG0480020927.01.T01 | MTR_2g084480 | 61.798 | 89 | 34 | 0 | 59 | 147 | 136 | 224 | 5.44e-37 | 126 |
| MsG0480020927.01.T01 | MTR_8g069990 | 67.742 | 93 | 22 | 3 | 15 | 107 | 4 | 88 | 1.48e-33 | 114 |
| MsG0480020927.01.T01 | MTR_4g063270 | 90.196 | 51 | 5 | 0 | 86 | 136 | 1 | 51 | 5.54e-27 | 95.9 |
| MsG0480020927.01.T01 | MTR_7g114580 | 90.244 | 41 | 4 | 0 | 108 | 148 | 11 | 51 | 9.26e-20 | 77.4 |
| MsG0480020927.01.T01 | MTR_4g032777 | 36.792 | 106 | 63 | 2 | 31 | 132 | 5 | 110 | 3.65e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020927.01.T01 | AT3G45980 | 88.742 | 151 | 13 | 2 | 1 | 148 | 1 | 150 | 2.11e-83 | 241 |
| MsG0480020927.01.T01 | AT2G28720 | 86.093 | 151 | 18 | 1 | 1 | 148 | 1 | 151 | 1.35e-80 | 234 |
| MsG0480020927.01.T01 | AT1G07790 | 86.275 | 153 | 11 | 3 | 1 | 148 | 1 | 148 | 1.25e-79 | 232 |
| MsG0480020927.01.T01 | AT5G59910 | 91.176 | 136 | 9 | 2 | 13 | 148 | 18 | 150 | 2.63e-77 | 226 |
| MsG0480020927.01.T01 | AT3G46030 | 94.444 | 126 | 6 | 1 | 23 | 148 | 21 | 145 | 1.07e-75 | 222 |
| MsG0480020927.01.T01 | AT2G37470 | 81.757 | 148 | 16 | 3 | 1 | 147 | 1 | 138 | 7.73e-71 | 209 |
| MsG0480020927.01.T01 | AT3G53650 | 81.879 | 149 | 15 | 3 | 1 | 148 | 1 | 138 | 1.02e-69 | 207 |
| MsG0480020927.01.T01 | AT5G22880 | 86.897 | 145 | 17 | 2 | 4 | 148 | 3 | 145 | 6.03e-67 | 200 |
| MsG0480020927.01.T01 | AT5G02570 | 79.730 | 148 | 14 | 1 | 1 | 148 | 1 | 132 | 1.14e-66 | 199 |
| MsG0480020927.01.T01 | AT3G09480 | 84.127 | 126 | 16 | 1 | 23 | 148 | 5 | 126 | 2.62e-62 | 187 |
| MsG0480020927.01.T01 | AT1G08170 | 56.989 | 93 | 40 | 0 | 53 | 145 | 143 | 235 | 1.79e-37 | 128 |
Find 43 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAGCTTCTTCCCTGCCTT+TGG | 0.316666 | 4:+50637394 | None:intergenic |
| GCTGTCAGGCTTGTTCTTCC+CGG | 0.318826 | 4:-50637127 | MsG0480020927.01.T01:CDS |
| TTGCTTGAGATACCAATGTC+TGG | 0.335174 | 4:+50637268 | None:intergenic |
| CTTCTCCGCCGGAGCTTTCT+CGG | 0.336863 | 4:+50637420 | None:intergenic |
| GCTTGTTCTTCCCGGTGAAT+TGG | 0.368237 | 4:-50637119 | MsG0480020927.01.T01:CDS |
| GCTGGCTTCTTCTCTCCCTT+TGG | 0.368494 | 4:+50637481 | None:intergenic |
| ATTTCCCTTGAAGTGATTGT+TGG | 0.372205 | 4:+50637157 | None:intergenic |
| CTTCTTCTTGTCTCCGGCAG+CGG | 0.399506 | 4:+50637357 | None:intergenic |
| GCCTTTGGCTTCTTCTCCGC+CGG | 0.402654 | 4:+50637409 | None:intergenic |
| CTCTGATTCAATGGCACCAA+AGG | 0.436385 | 4:-50637497 | None:intergenic |
| GCGGAGAAGAAGCCAAAGGC+AGG | 0.445065 | 4:-50637406 | MsG0480020927.01.T01:CDS |
| GTGGACTTCTTCTCCTCAGC+GGG | 0.452143 | 4:+50637448 | None:intergenic |
| AAGGAAGGTGGTTCCGCTGC+CGG | 0.458364 | 4:-50637370 | MsG0480020927.01.T01:CDS |
| TCAGCGGGTTTCTTCTCCGC+TGG | 0.467762 | 4:+50637463 | None:intergenic |
| GCAGCGGAACCACCTTCCTT+AGG | 0.468825 | 4:+50637373 | None:intergenic |
| GGGAAATTCAGACTGCTGTC+AGG | 0.472146 | 4:-50637141 | MsG0480020927.01.T01:CDS |
| ACCCTCAGAGACAGCATGCT+TGG | 0.475334 | 4:+50637096 | None:intergenic |
| AGCATGCTTGGCCAATTCAC+CGG | 0.481273 | 4:+50637108 | None:intergenic |
| GATCTAAGAACTAGTAAACT+TGG | 0.511385 | 4:+50637060 | None:intergenic |
| CACAGTCGCCGAGAAAGCTC+CGG | 0.525595 | 4:-50637428 | MsG0480020927.01.T01:CDS |
| TGCTTGAGATACCAATGTCT+GGG | 0.532971 | 4:+50637269 | None:intergenic |
| TGGTATCTCAAGCAAAGCCA+TGG | 0.533917 | 4:-50637260 | MsG0480020927.01.T01:CDS |
| GCATGCTTGGCCAATTCACC+GGG | 0.547334 | 4:+50637109 | None:intergenic |
| TCTGATTCAATGGCACCAAA+GGG | 0.547354 | 4:-50637496 | None:intergenic |
| AGTAAACTTGGTCACAGCCT+TGG | 0.548318 | 4:+50637072 | None:intergenic |
| GAAACTGTTCATAATTCCCA+TGG | 0.551655 | 4:+50637243 | None:intergenic |
| AGAAGCCAACAATCACTTCA+AGG | 0.558053 | 4:-50637162 | MsG0480020927.01.T01:CDS |
| CGGAGAAGAAGCCAAAGGCA+GGG | 0.564014 | 4:-50637405 | MsG0480020927.01.T01:CDS |
| GCCAAGCATGCTGTCTCTGA+GGG | 0.566391 | 4:-50637097 | MsG0480020927.01.T01:CDS |
| TGCTGTCTCTGAGGGTACCA+AGG | 0.572597 | 4:-50637089 | MsG0480020927.01.T01:CDS |
| GGCAGGGAAGAAGCTGCCTA+AGG | 0.584305 | 4:-50637389 | MsG0480020927.01.T01:CDS |
| GGCCAAGCATGCTGTCTCTG+AGG | 0.598295 | 4:-50637098 | MsG0480020927.01.T01:CDS |
| GGGAAGAAGCTGCCTAAGGA+AGG | 0.601312 | 4:-50637385 | MsG0480020927.01.T01:CDS |
| AAGCAAGTTCACCCAGACAT+TGG | 0.601452 | 4:-50637280 | MsG0480020927.01.T01:CDS |
| TCCGGCGGAGAAGAAGCCAA+AGG | 0.620050 | 4:-50637410 | MsG0480020927.01.T01:CDS |
| GAAGCCAACAATCACTTCAA+GGG | 0.625556 | 4:-50637161 | MsG0480020927.01.T01:CDS |
| AAGAAGCTGCCTAAGGAAGG+TGG | 0.636978 | 4:-50637382 | MsG0480020927.01.T01:CDS |
| CGGAGCTTTCTCGGCGACTG+TGG | 0.639450 | 4:+50637429 | None:intergenic |
| AAAGGGAGAGAAGAAGCCAG+CGG | 0.642633 | 4:-50637479 | MsG0480020927.01.T01:CDS |
| GGTATCTCAAGCAAAGCCAT+GGG | 0.654333 | 4:-50637259 | MsG0480020927.01.T01:CDS |
| TGTGGACTTCTTCTCCTCAG+CGG | 0.655377 | 4:+50637447 | None:intergenic |
| AGTCGCCGAGAAAGCTCCGG+CGG | 0.659369 | 4:-50637425 | MsG0480020927.01.T01:CDS |
| AGCGGAGAAGAAACCCGCTG+AGG | 0.708553 | 4:-50637461 | MsG0480020927.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTTCCCTTGAAGTGATTGT+TGG | + | Chr4:50637396-50637415 | None:intergenic | 35.0% | |
| GAAACTGTTCATAATTCCCA+TGG | + | Chr4:50637310-50637329 | None:intergenic | 35.0% | |
| ! | TCTTTTCTTCTTCTTGTCTC+CGG | + | Chr4:50637202-50637221 | None:intergenic | 35.0% |
| AGAAGCCAACAATCACTTCA+AGG | - | Chr4:50637388-50637407 | MsG0480020927.01.T01:CDS | 40.0% | |
| GAAGCCAACAATCACTTCAA+GGG | - | Chr4:50637389-50637408 | MsG0480020927.01.T01:CDS | 40.0% | |
| TGCTTGAGATACCAATGTCT+GGG | + | Chr4:50637284-50637303 | None:intergenic | 40.0% | |
| TTGCTTGAGATACCAATGTC+TGG | + | Chr4:50637285-50637304 | None:intergenic | 40.0% | |
| AAGCAAGTTCACCCAGACAT+TGG | - | Chr4:50637270-50637289 | MsG0480020927.01.T01:CDS | 45.0% | |
| AGTAAACTTGGTCACAGCCT+TGG | + | Chr4:50637481-50637500 | None:intergenic | 45.0% | |
| GGTATCTCAAGCAAAGCCAT+GGG | - | Chr4:50637291-50637310 | MsG0480020927.01.T01:CDS | 45.0% | |
| TGGTATCTCAAGCAAAGCCA+TGG | - | Chr4:50637290-50637309 | MsG0480020927.01.T01:CDS | 45.0% | |
| AAAGGGAGAGAAGAAGCCAG+CGG | - | Chr4:50637071-50637090 | MsG0480020927.01.T01:CDS | 50.0% | |
| AAGAAGCTGCCTAAGGAAGG+TGG | - | Chr4:50637168-50637187 | MsG0480020927.01.T01:CDS | 50.0% | |
| AGCATGCTTGGCCAATTCAC+CGG | + | Chr4:50637445-50637464 | None:intergenic | 50.0% | |
| GCTTGTTCTTCCCGGTGAAT+TGG | - | Chr4:50637431-50637450 | MsG0480020927.01.T01:CDS | 50.0% | |
| GGGAAATTCAGACTGCTGTC+AGG | - | Chr4:50637409-50637428 | MsG0480020927.01.T01:CDS | 50.0% | |
| TGTGGACTTCTTCTCCTCAG+CGG | + | Chr4:50637106-50637125 | None:intergenic | 50.0% | |
| ACCCTCAGAGACAGCATGCT+TGG | + | Chr4:50637457-50637476 | None:intergenic | 55.0% | |
| CGGAGAAGAAGCCAAAGGCA+GGG | - | Chr4:50637145-50637164 | MsG0480020927.01.T01:CDS | 55.0% | |
| CTTCTTCTTGTCTCCGGCAG+CGG | + | Chr4:50637196-50637215 | None:intergenic | 55.0% | |
| GCATGCTTGGCCAATTCACC+GGG | + | Chr4:50637444-50637463 | None:intergenic | 55.0% | |
| GCCAAGCATGCTGTCTCTGA+GGG | - | Chr4:50637453-50637472 | MsG0480020927.01.T01:CDS | 55.0% | |
| GGGAAGAAGCTGCCTAAGGA+AGG | - | Chr4:50637165-50637184 | MsG0480020927.01.T01:CDS | 55.0% | |
| GTGGACTTCTTCTCCTCAGC+GGG | + | Chr4:50637105-50637124 | None:intergenic | 55.0% | |
| ! | GCTGGCTTCTTCTCTCCCTT+TGG | + | Chr4:50637072-50637091 | None:intergenic | 55.0% |
| ! | GCTGTCAGGCTTGTTCTTCC+CGG | - | Chr4:50637423-50637442 | MsG0480020927.01.T01:CDS | 55.0% |
| !! | TGCTGTCTCTGAGGGTACCA+AGG | - | Chr4:50637461-50637480 | MsG0480020927.01.T01:CDS | 55.0% |
| AAGGAAGGTGGTTCCGCTGC+CGG | - | Chr4:50637180-50637199 | MsG0480020927.01.T01:CDS | 60.0% | |
| AGCGGAGAAGAAACCCGCTG+AGG | - | Chr4:50637089-50637108 | MsG0480020927.01.T01:CDS | 60.0% | |
| CACAGTCGCCGAGAAAGCTC+CGG | - | Chr4:50637122-50637141 | MsG0480020927.01.T01:CDS | 60.0% | |
| CTTCTCCGCCGGAGCTTTCT+CGG | + | Chr4:50637133-50637152 | None:intergenic | 60.0% | |
| GCAGCGGAACCACCTTCCTT+AGG | + | Chr4:50637180-50637199 | None:intergenic | 60.0% | |
| GCGGAGAAGAAGCCAAAGGC+AGG | - | Chr4:50637144-50637163 | MsG0480020927.01.T01:CDS | 60.0% | |
| GGCAGCTTCTTCCCTGCCTT+TGG | + | Chr4:50637159-50637178 | None:intergenic | 60.0% | |
| GGCAGGGAAGAAGCTGCCTA+AGG | - | Chr4:50637161-50637180 | MsG0480020927.01.T01:CDS | 60.0% | |
| GGCCAAGCATGCTGTCTCTG+AGG | - | Chr4:50637452-50637471 | MsG0480020927.01.T01:CDS | 60.0% | |
| TCCGGCGGAGAAGAAGCCAA+AGG | - | Chr4:50637140-50637159 | MsG0480020927.01.T01:CDS | 60.0% | |
| ! | GCCTTTGGCTTCTTCTCCGC+CGG | + | Chr4:50637144-50637163 | None:intergenic | 60.0% |
| ! | TCAGCGGGTTTCTTCTCCGC+TGG | + | Chr4:50637090-50637109 | None:intergenic | 60.0% |
| AGTCGCCGAGAAAGCTCCGG+CGG | - | Chr4:50637125-50637144 | MsG0480020927.01.T01:CDS | 65.0% | |
| CGGAGCTTTCTCGGCGACTG+TGG | + | Chr4:50637124-50637143 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 50637063 | 50637509 | 50637063 | ID=MsG0480020927.01;Name=MsG0480020927.01 |
| Chr4 | mRNA | 50637063 | 50637509 | 50637063 | ID=MsG0480020927.01.T01;Parent=MsG0480020927.01;Name=MsG0480020927.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|148 |
| Chr4 | exon | 50637063 | 50637509 | 50637063 | ID=MsG0480020927.01.T01:exon:329;Parent=MsG0480020927.01.T01 |
| Chr4 | CDS | 50637063 | 50637509 | 50637063 | ID=MsG0480020927.01.T01:cds;Parent=MsG0480020927.01.T01 |
| Gene Sequence |
| Protein sequence |
