AlfalfaGEDB | MsG0480020918.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020918.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020918.01.T01	XP_003606627.1	98.649	148	1	1	1	147	1	148	1.28E-95	283

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020918.01.T01	Q1S9I9	98.649	148	1	1	1	147	1	148	3.33E-98	282

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020918.01.T01	G7JTQ7	98.649	148	1	1	1	147	1	148	6.13e-96	283

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004994.01	MsG0480020918.01	0.811002	8.702004e-51	5.325723e-48
MsG0180005048.01	MsG0480020918.01	0.808088	3.677882e-50	2.083667e-47
MsG0380014684.01	MsG0480020918.01	0.812716	3.685306e-51	2.360408e-48
MsG0480020918.01	MsG0480020920.01	0.804790	1.823655e-49	9.489114e-47
MsG0480020918.01	MsG0480020927.01	0.855771	5.044990e-62	1.177741e-58
MsG0480020918.01	MsG0480020971.01	0.831478	1.631496e-55	1.772655e-52
MsG0480020918.01	MsG0480021015.01	0.894989	1.466712e-75	1.492928e-71
MsG0480020918.01	MsG0480021252.01	0.847127	1.407418e-59	2.477065e-56
MsG0480020918.01	MsG0480021302.01	0.858283	9.159848e-63	2.325703e-59
MsG0480020918.01	MsG0580025506.01	0.802780	4.769393e-49	2.356829e-46

PPI

Gene1	Gene2	Type
MsG0480020918.01	MsG0880047260.01	PPI
MsG0480020918.01	MsG0580027231.01	PPI
MsG0480020918.01	MsG0580029194.01	PPI
MsG0480020918.01	MsG0580029044.01	PPI
MsG0580025506.01	MsG0480020918.01	PPI
MsG0480020918.01	MsG0480023842.01	PPI
MsG0480020918.01	MsG0880045015.01	PPI
MsG0480020918.01	MsG0780038642.01	PPI
MsG0480020918.01	MsG0880042844.01	PPI
MsG0480020918.01	MsG0780041592.01	PPI
MsG0480020918.01	MsG0380012788.01	PPI
MsG0480020918.01	MsG0780041593.01	PPI
MsG0480020918.01	MsG0480022307.01	PPI
MsG0480020918.01	MsG0480020877.01	PPI
MsG0480020918.01	MsG0880044910.01	PPI
MsG0480020918.01	MsG0280007919.01	PPI
MsG0480020918.01	MsG0580025504.01	PPI
MsG0480020918.01	MsG0280008380.01	PPI
MsG0480020918.01	MsG0180000243.01	PPI
MsG0880044910.01	MsG0480020918.01	PPI
MsG0480020918.01	MsG0780036534.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020918.01.T01	MTR_4g063220	98.649	148	1	1	1	147	1	148	1.55e-99	283
MsG0480020918.01.T01	MTR_4g063990	98.649	148	1	1	1	147	1	148	1.55e-99	283
MsG0480020918.01.T01	MTR_4g063200	98.649	148	1	1	1	147	1	148	1.55e-99	283
MsG0480020918.01.T01	MTR_4g064020	98.649	148	1	1	1	147	1	148	1.55e-99	283
MsG0480020918.01.T01	MTR_4g071180	98.649	148	1	1	1	147	1	148	3.24e-99	282
MsG0480020918.01.T01	MTR_4g070240	98.649	148	1	1	1	147	1	148	3.24e-99	282
MsG0480020918.01.T01	MTR_4g070245	97.297	148	3	1	1	147	1	148	1.20e-97	278
MsG0480020918.01.T01	MTR_4g063240	97.973	148	1	2	1	147	1	147	3.32e-97	277
MsG0480020918.01.T01	MTR_4g063275	95.946	148	5	1	1	147	1	148	4.05e-96	275
MsG0480020918.01.T01	MTR_4g064983	97.241	145	3	1	1	144	1	145	7.16e-94	272
MsG0480020918.01.T01	MTR_4g088025	94.558	147	4	1	1	147	1	143	1.70e-88	255
MsG0480020918.01.T01	MTR_1g090780	87.838	148	6	4	1	147	1	137	9.95e-76	223
MsG0480020918.01.T01	MTR_1g492910	87.162	148	7	4	1	147	1	137	4.42e-75	221
MsG0480020918.01.T01	MTR_1g068600	92.793	111	4	1	37	147	30	136	2.73e-63	191
MsG0480020918.01.T01	MTR_7g099960	92.793	111	5	1	37	147	31	138	5.32e-63	191
MsG0480020918.01.T01	MTR_2g084480	61.798	89	34	0	58	146	136	224	4.80e-36	125
MsG0480020918.01.T01	MTR_8g069990	68.817	93	21	3	14	106	4	88	1.26e-33	115
MsG0480020918.01.T01	MTR_4g063270	90.196	51	5	0	85	135	1	51	2.45e-26	95.1
MsG0480020918.01.T01	MTR_7g114580	90.244	41	4	0	107	147	11	51	3.23e-19	76.6
MsG0480020918.01.T01	MTR_4g032777	36.792	106	63	2	30	131	5	110	1.35e-13	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020918.01.T01	AT3G45980	88.079	151	13	3	1	147	1	150	8.94e-81	236
MsG0480020918.01.T01	AT2G28720	86.093	151	17	2	1	147	1	151	3.36e-79	232
MsG0480020918.01.T01	AT1G07790	86.093	151	14	3	1	147	1	148	5.25e-77	226
MsG0480020918.01.T01	AT5G59910	90.511	137	10	2	11	147	17	150	1.01e-76	226
MsG0480020918.01.T01	AT3G46030	94.444	126	6	1	22	147	21	145	4.58e-75	221
MsG0480020918.01.T01	AT2G37470	82.877	146	17	3	1	146	1	138	2.05e-69	207
MsG0480020918.01.T01	AT3G53650	80.952	147	19	3	1	147	1	138	2.02e-67	202
MsG0480020918.01.T01	AT5G22880	86.395	147	18	2	1	147	1	145	1.28e-65	197
MsG0480020918.01.T01	AT5G02570	81.633	147	12	3	1	147	1	132	2.94e-64	194
MsG0480020918.01.T01	AT3G09480	84.127	126	16	1	22	147	5	126	9.67e-62	187
MsG0480020918.01.T01	AT1G08170	59.770	87	35	0	58	144	149	235	2.41e-36	126

Find 46 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCTACTTTATCTGATATT+AGG	0.189131	4:+50555046	MsG0480020918.01.T01:CDS
TTCCTTTGGTAGCTTCTTTC+CGG	0.190600	4:-50554675	None:intergenic
AGTTCTAGATTGACTTTATT+GGG	0.272182	4:+50555013	MsG0480020918.01.T01:CDS
TAGTTCTAGATTGACTTTAT+TGG	0.280701	4:+50555012	MsG0480020918.01.T01:CDS
GGTAGCTTCTTTCCGGCCTT+TGG	0.319499	4:-50554668	None:intergenic
TTGCTTGAGATACCAATGTC+TGG	0.335174	4:-50554794	None:intergenic
CTTCTCCGCCGGAGCTTTCT+CGG	0.336863	4:-50554642	None:intergenic
ACTTGTTCTTCCTGGTGAAT+TGG	0.346831	4:+50554943	MsG0480020918.01.T01:CDS
GCTGTCAGACTTGTTCTTCC+TGG	0.349421	4:+50554935	MsG0480020918.01.T01:CDS
TCCGCTGGCTTCTCTCCCTT+TGG	0.351023	4:-50554584	None:intergenic
CTTCTTCTTGTCTCCGGCAG+CGG	0.399506	4:-50554705	None:intergenic
ATTTCCCTGGAAGTGATTGT+TGG	0.430207	4:-50554905	None:intergenic
GCAGCGGAACCTCCTTCCTT+TGG	0.430241	4:-50554689	None:intergenic
GCCTTTGGCTTCTTCTCCGC+CGG	0.441924	4:-50554653	None:intergenic
GTGGACTTCTTCTCCTCAGC+GGG	0.452143	4:-50554614	None:intergenic
TCAGCGGGTTTCTTCTCCGC+TGG	0.467762	4:-50554599	None:intergenic
TCTCTTCTTCTTCTTGTCTC+CGG	0.474508	4:-50554711	None:intergenic
AGAAGCCAACAATCACTTCC+AGG	0.480188	4:+50554900	MsG0480020918.01.T01:CDS
AAGGAAGGAGGTTCCGCTGC+CGG	0.482012	4:+50554692	MsG0480020918.01.T01:CDS
CGCCGGAGCTTTCTCGGCGA+CGG	0.491905	4:-50554636	None:intergenic
CAATTCTCAAATGGCACCAA+AGG	0.498443	4:+50554568	None:intergenic
GACAGCGGTCTGAATTTCCC+TGG	0.510547	4:-50554918	None:intergenic
CACCGTCGCCGAGAAAGCTC+CGG	0.523576	4:+50554634	MsG0480020918.01.T01:CDS
GCGGAGAAGAAGCCAAAGGC+CGG	0.524477	4:+50554656	MsG0480020918.01.T01:CDS
GCTAAGCATGCCGTCTCTGA+AGG	0.527180	4:+50554965	MsG0480020918.01.T01:CDS
TGCTTGAGATACCAATGTCT+GGG	0.532971	4:-50554793	None:intergenic
TGGTATCTCAAGCAAAGCCA+TGG	0.533917	4:+50554802	MsG0480020918.01.T01:CDS
AATTCTCAAATGGCACCAAA+GGG	0.565558	4:+50554569	None:intergenic
AGCCTTGGTGCCTTCAGAGA+CGG	0.568406	4:-50554975	None:intergenic
GGAAAGAAGCTACCAAAGGA+AGG	0.598625	4:+50554677	MsG0480020918.01.T01:CDS
AGTAAACTTAGTCACAGCCT+TGG	0.601904	4:-50554990	None:intergenic
TGCCGTCTCTGAAGGCACCA+AGG	0.610469	4:+50554973	MsG0480020918.01.T01:CDS
GCATGCTTAGCCAATTCACC+AGG	0.611062	4:-50554953	None:intergenic
GGCCGGAAAGAAGCTACCAA+AGG	0.611990	4:+50554673	MsG0480020918.01.T01:CDS
AAACAAGTTCACCCAGACAT+TGG	0.612279	4:+50554782	MsG0480020918.01.T01:CDS
GAAACTGTTCATGATTCCCA+TGG	0.633384	4:-50554819	None:intergenic
GAAGCCAACAATCACTTCCA+GGG	0.639164	4:+50554901	MsG0480020918.01.T01:CDS
CGTCGCCGAGAAAGCTCCGG+CGG	0.643171	4:+50554637	MsG0480020918.01.T01:CDS
TCCGGCGGAGAAGAAGCCAA+AGG	0.649331	4:+50554652	MsG0480020918.01.T01:CDS
CGGAGCTTTCTCGGCGACGG+TGG	0.651215	4:-50554633	None:intergenic
GGTATCTCAAGCAAAGCCAT+GGG	0.654333	4:+50554803	MsG0480020918.01.T01:CDS
ACCAAAGGGAGAGAAGCCAG+CGG	0.663205	4:+50554583	MsG0480020918.01.T01:CDS
AAGAAGCTACCAAAGGAAGG+AGG	0.671333	4:+50554680	MsG0480020918.01.T01:CDS
GGTGGACTTCTTCTCCTCAG+CGG	0.696334	4:-50554615	None:intergenic
AGCGGAGAAGAAACCCGCTG+AGG	0.708553	4:+50554601	MsG0480020918.01.T01:CDS
AGGAAGAACAAGTCTGACAG+CGG	0.732527	4:-50554933	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTTCTAGATTGACTTTATT+GGG	+	Chr4:50555013-50555032	MsG0480020918.01.T01:CDS	25.0%
!!!	ATGCTACTTTATCTGATATT+AGG	+	Chr4:50555046-50555065	MsG0480020918.01.T01:CDS	25.0%
!!!	TAGTTCTAGATTGACTTTAT+TGG	+	Chr4:50555012-50555031	MsG0480020918.01.T01:CDS	25.0%
	AAACAAGTTCACCCAGACAT+TGG	+	Chr4:50554782-50554801	MsG0480020918.01.T01:CDS	40.0%
	ACTTGTTCTTCCTGGTGAAT+TGG	+	Chr4:50554943-50554962	MsG0480020918.01.T01:CDS	40.0%
	AGTAAACTTAGTCACAGCCT+TGG	-	Chr4:50554993-50555012	None:intergenic	40.0%
	ATTTCCCTGGAAGTGATTGT+TGG	-	Chr4:50554908-50554927	None:intergenic	40.0%
	GAAACTGTTCATGATTCCCA+TGG	-	Chr4:50554822-50554841	None:intergenic	40.0%
	TCTCTTCTTCTTCTTGTCTC+CGG	-	Chr4:50554714-50554733	None:intergenic	40.0%
	TGCTTGAGATACCAATGTCT+GGG	-	Chr4:50554796-50554815	None:intergenic	40.0%
	TTGCTTGAGATACCAATGTC+TGG	-	Chr4:50554797-50554816	None:intergenic	40.0%
!	TTCCTTTGGTAGCTTCTTTC+CGG	-	Chr4:50554678-50554697	None:intergenic	40.0%
	AAGAAGCTACCAAAGGAAGG+AGG	+	Chr4:50554680-50554699	MsG0480020918.01.T01:CDS	45.0%
	AGAAGCCAACAATCACTTCC+AGG	+	Chr4:50554900-50554919	MsG0480020918.01.T01:CDS	45.0%
	AGGAAGAACAAGTCTGACAG+CGG	-	Chr4:50554936-50554955	None:intergenic	45.0%
	GAAGCCAACAATCACTTCCA+GGG	+	Chr4:50554901-50554920	MsG0480020918.01.T01:CDS	45.0%
	GGAAAGAAGCTACCAAAGGA+AGG	+	Chr4:50554677-50554696	MsG0480020918.01.T01:CDS	45.0%
	GGTATCTCAAGCAAAGCCAT+GGG	+	Chr4:50554803-50554822	MsG0480020918.01.T01:CDS	45.0%
	TGGTATCTCAAGCAAAGCCA+TGG	+	Chr4:50554802-50554821	MsG0480020918.01.T01:CDS	45.0%
	GCATGCTTAGCCAATTCACC+AGG	-	Chr4:50554956-50554975	None:intergenic	50.0%
!	GCTGTCAGACTTGTTCTTCC+TGG	+	Chr4:50554935-50554954	MsG0480020918.01.T01:CDS	50.0%
	ACCAAAGGGAGAGAAGCCAG+CGG	+	Chr4:50554583-50554602	MsG0480020918.01.T01:CDS	55.0%
	CTTCTTCTTGTCTCCGGCAG+CGG	-	Chr4:50554708-50554727	None:intergenic	55.0%
	GACAGCGGTCTGAATTTCCC+TGG	-	Chr4:50554921-50554940	None:intergenic	55.0%
	GCTAAGCATGCCGTCTCTGA+AGG	+	Chr4:50554965-50554984	MsG0480020918.01.T01:CDS	55.0%
	GGCCGGAAAGAAGCTACCAA+AGG	+	Chr4:50554673-50554692	MsG0480020918.01.T01:CDS	55.0%
	GGTAGCTTCTTTCCGGCCTT+TGG	-	Chr4:50554671-50554690	None:intergenic	55.0%
	GTGGACTTCTTCTCCTCAGC+GGG	-	Chr4:50554617-50554636	None:intergenic	55.0%
!	GGTGGACTTCTTCTCCTCAG+CGG	-	Chr4:50554618-50554637	None:intergenic	55.0%
!!	AGCCTTGGTGCCTTCAGAGA+CGG	-	Chr4:50554978-50554997	None:intergenic	55.0%
	AAGGAAGGAGGTTCCGCTGC+CGG	+	Chr4:50554692-50554711	MsG0480020918.01.T01:CDS	60.0%
	AGCGGAGAAGAAACCCGCTG+AGG	+	Chr4:50554601-50554620	MsG0480020918.01.T01:CDS	60.0%
	CTTCTCCGCCGGAGCTTTCT+CGG	-	Chr4:50554645-50554664	None:intergenic	60.0%
	GCAGCGGAACCTCCTTCCTT+TGG	-	Chr4:50554692-50554711	None:intergenic	60.0%
	GCGGAGAAGAAGCCAAAGGC+CGG	+	Chr4:50554656-50554675	MsG0480020918.01.T01:CDS	60.0%
	TCCGGCGGAGAAGAAGCCAA+AGG	+	Chr4:50554652-50554671	MsG0480020918.01.T01:CDS	60.0%
!	GCCTTTGGCTTCTTCTCCGC+CGG	-	Chr4:50554656-50554675	None:intergenic	60.0%
!	TCAGCGGGTTTCTTCTCCGC+TGG	-	Chr4:50554602-50554621	None:intergenic	60.0%
!!	TCCGCTGGCTTCTCTCCCTT+TGG	-	Chr4:50554587-50554606	None:intergenic	60.0%
!!	TGCCGTCTCTGAAGGCACCA+AGG	+	Chr4:50554973-50554992	MsG0480020918.01.T01:CDS	60.0%
	CACCGTCGCCGAGAAAGCTC+CGG	+	Chr4:50554634-50554653	MsG0480020918.01.T01:CDS	65.0%
	CGCCGGAGCTTTCTCGGCGA+CGG	-	Chr4:50554639-50554658	None:intergenic	70.0%
	CGGAGCTTTCTCGGCGACGG+TGG	-	Chr4:50554636-50554655	None:intergenic	70.0%
	CGTCGCCGAGAAAGCTCCGG+CGG	+	Chr4:50554637-50554656	MsG0480020918.01.T01:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	50554578	50555090	50554578	ID=MsG0480020918.01;Name=MsG0480020918.01
Chr4	mRNA	50554578	50555090	50554578	ID=MsG0480020918.01.T01;Parent=MsG0480020918.01;Name=MsG0480020918.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|170
Chr4	exon	50554578	50555090	50554578	ID=MsG0480020918.01.T01:exon:16639;Parent=MsG0480020918.01.T01
Chr4	CDS	50554578	50555090	50554578	ID=MsG0480020918.01.T01:cds;Parent=MsG0480020918.01.T01

Gene Sequence

>MsG0480020918.01.T01

ATGGCACCAAAGGGAGAGAAGCCAGCGGAGAAGAAACCCGCTGAGGAGAAGAAGTCCACCGTCGCCGAGAAAGCTCCGGCGGAGAAGAAGCCAAAGGCCGGAAAGAAGCTACCAAAGGAAGGAGGTTCCGCTGCCGGAGACAAGAAGAAGAAGAGAAGCAAGAAGAGCGTTGAAACATACAAGATCTACATATTCAAAGTTCTGAAACAAGTTCACCCAGACATTGGTATCTCAAGCAAAGCCATGGGAATCATGAACAGTTTCATCAATGATATCTTCGAGAAGCTTGCTCAAGAATCATCAAGACTTGCTCGCTACAACAAGAAGCCAACAATCACTTCCAGGGAAATTCAGACCGCTGTCAGACTTGTTCTTCCTGGTGAATTGGCTAAGCATGCCGTCTCTGAAGGCACCAAGGCTGTGACTAAGTTTACTAGTTCTAGATTGACTTTATTGGGATCTTGTTTGATGCTACTTTATCTGATATTAGGAATTGTTAGATGTTTTAATTAG

Protein sequence

>MsG0480020918.01.T01

MAPKGEKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSSRLTLLGSCLMLLYLILGIVRCFN*