AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041172.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041172.01.T01 MTR_7g105810 100.000 191 0 0 1 191 1 191 1.03e-143 397
MsG0780041172.01.T01 MTR_7g105810 100.000 191 0 0 1 191 14 204 1.13e-143 398
MsG0780041172.01.T01 MTR_7g105810 100.000 156 0 0 1 156 1 156 1.25e-115 325
MsG0780041172.01.T01 MTR_7g105810 100.000 147 0 0 45 191 12 158 3.33e-107 304
MsG0780041172.01.T01 MTR_1g057940 60.209 191 69 2 1 191 1 184 1.47e-84 251
MsG0780041172.01.T01 MTR_3g113790 46.927 179 85 1 1 169 151 329 6.78e-62 198
MsG0780041172.01.T01 MTR_3g113790 54.795 146 66 0 1 146 151 296 4.54e-61 192
MsG0780041172.01.T01 MTR_1g024930 46.154 169 88 1 1 166 1 169 5.28e-56 180
MsG0780041172.01.T01 MTR_5g019720 27.451 102 72 1 6 107 28 127 9.58e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041172.01.T01 AT2G36020 45.854 205 95 2 1 189 1 205 1.03e-64 200
MsG0780041172.01.T01 AT5G42560 48.571 175 86 2 1 172 1 174 1.55e-62 196
MsG0780041172.01.T01 AT1G75700 42.935 184 95 1 1 184 1 174 2.32e-58 181
MsG0780041172.01.T01 AT1G75700 42.935 184 95 1 1 184 1 174 2.32e-58 181
MsG0780041172.01.T01 AT5G42560 48.171 164 81 2 12 172 1 163 7.92e-57 181
MsG0780041172.01.T01 AT5G42560 48.171 164 81 2 12 172 1 163 7.92e-57 181
MsG0780041172.01.T01 AT1G19950 49.315 146 74 0 1 146 1 146 3.42e-55 177
MsG0780041172.01.T01 AT1G75700 41.398 186 80 1 1 157 1 186 6.91e-52 166
MsG0780041172.01.T01 AT4G36720 24.038 104 77 1 4 107 34 135 9.25e-11 58.9

Find 49 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTTCTTACCTTAGTTT+TGG 0.186305 7:-87568496 None:intergenic
AAATGGATGAACTTCGTTTC+TGG 0.202259 7:+87567808 MsG0780041172.01.T01:CDS
AACAATGCTGATCGTATTTC+AGG 0.224707 7:-87569365 None:intergenic
TTTCAAGAGTGGAGAGTTAG+AGG 0.253437 7:+87568746 MsG0780041172.01.T01:CDS
GTTTCTGCAACTCACCTTCT+TGG 0.276446 7:-87568882 None:intergenic
TTTAATTTCAGATTGCTTCT+TGG 0.286260 7:+87567732 MsG0780041172.01.T01:intron
TGCGGCCCACGAAATCATTT+TGG 0.289822 7:-87569397 None:intergenic
CACCTTCTTGGAGGATTTGC+CGG 0.306984 7:-87568870 None:intergenic
AAGTTTGCAGATGTGGTTAT+TGG 0.307274 7:+87568326 MsG0780041172.01.T01:CDS
TTCTAAGGTTTCAAAATGTT+AGG 0.337083 7:+87567555 None:intergenic
TGGCAAAATTGCACTGAGTT+GGG 0.337616 7:+87568791 MsG0780041172.01.T01:CDS
AAGAGTGGAGAGTTAGAGGA+TGG 0.360232 7:+87568750 MsG0780041172.01.T01:CDS
GAGAGTTAGAGGATGGGATT+TGG 0.375083 7:+87568757 MsG0780041172.01.T01:CDS
CTTCTTGGATATGCATATCC+TGG 0.386912 7:+87567747 MsG0780041172.01.T01:CDS
TTCGTTTCTGGTGTCAATAC+TGG 0.390648 7:+87567820 MsG0780041172.01.T01:CDS
ATTTCATTCTTAGATACATA+TGG 0.391382 7:-87568707 None:intergenic
CTGGCAAAATTGCACTGAGT+TGG 0.413346 7:+87568790 MsG0780041172.01.T01:CDS
TCTAAGGTTTCAAAATGTTA+GGG 0.416787 7:+87567556 None:intergenic
GATACCACATGTATATGAAC+AGG 0.423288 7:-87568473 None:intergenic
TGAGAGAAACAAGGTTGAAA+TGG 0.432547 7:+87567791 MsG0780041172.01.T01:CDS
GGATTTGCCGGATAATCTCT+TGG 0.433537 7:-87568858 None:intergenic
AATGGTGGGGCACTAGGAAC+AGG 0.434278 7:-87569341 None:intergenic
AAGCTTCAATTCTCCATACA+TGG 0.464555 7:-87568448 None:intergenic
GTGGGCCGCAAGAAGAAGTA+AGG 0.470213 7:+87569410 MsG0780041172.01.T01:CDS
GCTCAATCCAAGAGATTATC+CGG 0.480841 7:+87568851 MsG0780041172.01.T01:CDS
AGAGTGGAGAGTTAGAGGAT+GGG 0.481690 7:+87568751 MsG0780041172.01.T01:CDS
TCAGGCAATGGTGGGGCACT+AGG 0.483166 7:-87569347 None:intergenic
ATCGTATTTCAGGCAATGGT+GGG 0.489379 7:-87569355 None:intergenic
TTCAACCTTGTTTCTCTCAA+CGG 0.490268 7:-87567787 None:intergenic
GATCGTATTTCAGGCAATGG+TGG 0.496144 7:-87569356 None:intergenic
ACTGGAAAAGTTTGCAGATG+TGG 0.501953 7:+87568319 MsG0780041172.01.T01:CDS
TCTGCAACTCACCTTCTTGG+AGG 0.506808 7:-87568879 None:intergenic
GGCAAAATTGCACTGAGTTG+GGG 0.543534 7:+87568792 MsG0780041172.01.T01:CDS
CATGTGGTATCCCAAAACTA+AGG 0.546302 7:+87568485 MsG0780041172.01.T01:CDS
TTGACAAGAAGTTTCAAGAG+TGG 0.546722 7:+87568735 MsG0780041172.01.T01:CDS
TGTTGCTTGATGTAGGGGAC+AGG 0.552380 7:+87568659 MsG0780041172.01.T01:intron
ACTCCACCAAAATGATTTCG+TGG 0.565388 7:+87569391 MsG0780041172.01.T01:CDS
GCTGATCGTATTTCAGGCAA+TGG 0.578265 7:-87569359 None:intergenic
ATCCGGCAAATCCTCCAAGA+AGG 0.603972 7:+87568868 MsG0780041172.01.T01:CDS
CTAAGGTTTCAAAATGTTAG+GGG 0.607844 7:+87567557 None:intergenic
AGGTCCCTTACTTCTTCTTG+CGG 0.609032 7:-87569415 None:intergenic
AGCTTCAATTCTCCATACAT+GGG 0.610707 7:-87568447 None:intergenic
TTGCAGATGTGGTTATTGGA+TGG 0.618078 7:+87568330 MsG0780041172.01.T01:CDS
CTCCACCAAAATGATTTCGT+GGG 0.622122 7:+87569392 MsG0780041172.01.T01:CDS
TGGGCCGCAAGAAGAAGTAA+GGG 0.646170 7:+87569411 MsG0780041172.01.T01:CDS
CAAAACCGTTGAGAGAAACA+AGG 0.662480 7:+87567782 MsG0780041172.01.T01:CDS
TTGCCCTGTTCATATACATG+TGG 0.670217 7:+87568469 MsG0780041172.01.T01:CDS
TCGTATTTCAGGCAATGGTG+GGG 0.678523 7:-87569354 None:intergenic
ATACCACATGTATATGAACA+GGG 0.722802 7:-87568472 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTAGTCAATTTAAAAA+TGG - Chr7:87568915-87568934 None:intergenic 15.0%
!!! TGTTTAAAGTAAAAAGAAAT+AGG + Chr7:87568014-87568033 MsG0780041172.01.T01:intron 15.0%
!! ATAATTATATGCTATGCAAA+TGG + Chr7:87568222-87568241 MsG0780041172.01.T01:intron 20.0%
!! ATTTCATTCTTAGATACATA+TGG - Chr7:87568710-87568729 None:intergenic 20.0%
!! TTTGTACAAAAAATTAACGT+TGG + Chr7:87568408-87568427 MsG0780041172.01.T01:intron 20.0%
!!! AAACGTTTTAAATTAGCTAT+TGG - Chr7:87569084-87569103 None:intergenic 20.0%
!!! AAGCAATGATTTAAAATTGA+TGG - Chr7:87569235-87569254 None:intergenic 20.0%
!!! AATTTCTTTTACATTTACCA+AGG + Chr7:87568592-87568611 MsG0780041172.01.T01:intron 20.0%
!!! ATTATTTTGCAGAAAAAAGA+TGG + Chr7:87569311-87569330 MsG0780041172.01.T01:intron 20.0%
!!! CTTTTTAAATGGTTAAATCA+AGG + Chr7:87568097-87568116 MsG0780041172.01.T01:intron 20.0%
!!! TAATTTAAAACGTTTCCAAA+TGG + Chr7:87569089-87569108 MsG0780041172.01.T01:intron 20.0%
!!! TCATTTTTTAAAATCATCAC+CGG + Chr7:87569022-87569041 MsG0780041172.01.T01:intron 20.0%
!!! TTATTTTGCAGAAAAAAGAT+GGG + Chr7:87569312-87569331 MsG0780041172.01.T01:intron 20.0%
! AAGGTCAATAACTTAAAAAG+CGG + Chr7:87568116-87568135 MsG0780041172.01.T01:intron 25.0%
! ATGGAGTTAATAATCATTGT+TGG + Chr7:87568241-87568260 MsG0780041172.01.T01:intron 25.0%
! TACAATGATCCTACAAATTA+AGG - Chr7:87568285-87568304 None:intergenic 25.0%
!! TTTAATTTCAGATTGCTTCT+TGG + Chr7:87567732-87567751 MsG0780041172.01.T01:intron 25.0%
!!! AAACAGATCTGCTTTTTAAA+TGG + Chr7:87568086-87568105 MsG0780041172.01.T01:intron 25.0%
!!! TTTGTTCATCCTTAATTTGT+AGG + Chr7:87568273-87568292 MsG0780041172.01.T01:intron 25.0%
!!! TTTTCAAGTTATTGATCACT+TGG + Chr7:87568826-87568845 MsG0780041172.01.T01:CDS 25.0%
AAATTGCACTGAAATTGTTG+TGG + Chr7:87567965-87567984 MsG0780041172.01.T01:intron 30.0%
AACATAACTTTCACTTTCCT+TGG - Chr7:87568612-87568631 None:intergenic 30.0%
AATGAAGATGTAGAAGAAGA+AGG - Chr7:87567867-87567886 None:intergenic 30.0%
ATACCACATGTATATGAACA+GGG - Chr7:87568475-87568494 None:intergenic 30.0%
ATGCTTAAGACAGTAACTAT+TGG + Chr7:87568525-87568544 MsG0780041172.01.T01:intron 30.0%
ATTATCCTTAGTAACAGTAC+TGG - Chr7:87569291-87569310 None:intergenic 30.0%
CAAACAAAACATACTGAATC+AGG + Chr7:87569168-87569187 MsG0780041172.01.T01:intron 30.0%
TGCTTAAGACAGTAACTATT+GGG + Chr7:87568526-87568545 MsG0780041172.01.T01:intron 30.0%
! AGATAAATGTTGCTTGATGT+AGG + Chr7:87568652-87568671 MsG0780041172.01.T01:intron 30.0%
! ATAAATGTTGCTTGATGTAG+GGG + Chr7:87568654-87568673 MsG0780041172.01.T01:intron 30.0%
! CATATGTGTTGATGTTATGT+CGG - Chr7:87568986-87569005 None:intergenic 30.0%
! GATAAATGTTGCTTGATGTA+GGG + Chr7:87568653-87568672 MsG0780041172.01.T01:intron 30.0%
! TAAAAAGCAGATCTGTTTCT+TGG - Chr7:87568084-87568103 None:intergenic 30.0%
! TGTTCTTCTTACCTTAGTTT+TGG - Chr7:87568499-87568518 None:intergenic 30.0%
! TTAACGTTGGAACTTTTTGA+AGG + Chr7:87568421-87568440 MsG0780041172.01.T01:intron 30.0%
!! CTGATTCAGTATGTTTTGTT+TGG - Chr7:87569170-87569189 None:intergenic 30.0%
!! GTTCTTCTTACCTTAGTTTT+GGG - Chr7:87568498-87568517 None:intergenic 30.0%
AAATGGATGAACTTCGTTTC+TGG + Chr7:87567808-87567827 MsG0780041172.01.T01:CDS 35.0%
AACAATGCTGATCGTATTTC+AGG - Chr7:87569368-87569387 None:intergenic 35.0%
AAGCTTCAATTCTCCATACA+TGG - Chr7:87568451-87568470 None:intergenic 35.0%
AAGTTTGCAGATGTGGTTAT+TGG + Chr7:87568326-87568345 MsG0780041172.01.T01:CDS 35.0%
ACAGTTGAGATTAAGCCATT+TGG - Chr7:87569107-87569126 None:intergenic 35.0%
AGCTTCAATTCTCCATACAT+GGG - Chr7:87568450-87568469 None:intergenic 35.0%
GATACCACATGTATATGAAC+AGG - Chr7:87568476-87568495 None:intergenic 35.0%
TGAGAGAAACAAGGTTGAAA+TGG + Chr7:87567791-87567810 MsG0780041172.01.T01:CDS 35.0%
TGCAGAGAAGAATCAGAAAA+TGG - Chr7:87567642-87567661 None:intergenic 35.0%
TTGACAAGAAGTTTCAAGAG+TGG + Chr7:87568735-87568754 MsG0780041172.01.T01:CDS 35.0%
! ACCAAACATGCTTCTGAAAA+AGG - Chr7:87568169-87568188 None:intergenic 35.0%
! GTTTTGTAGCATTCAAATCC+AGG - Chr7:87567768-87567787 None:intergenic 35.0%
! TTCAACCTTGTTTCTCTCAA+CGG - Chr7:87567790-87567809 None:intergenic 35.0%
!!! ACCTTTTTCAGAAGCATGTT+TGG + Chr7:87568165-87568184 MsG0780041172.01.T01:intron 35.0%
!!! GGGATTTGGCAATTTTTTAC+TGG + Chr7:87568771-87568790 MsG0780041172.01.T01:CDS 35.0%
!!! TGTAGCTTTTTTCACAGTAC+TGG + Chr7:87568301-87568320 MsG0780041172.01.T01:CDS 35.0%
ACTCCACCAAAATGATTTCG+TGG + Chr7:87569391-87569410 MsG0780041172.01.T01:CDS 40.0%
ACTGAAATTGTTGTGGATCG+TGG + Chr7:87567972-87567991 MsG0780041172.01.T01:intron 40.0%
ACTGGAAAAGTTTGCAGATG+TGG + Chr7:87568319-87568338 MsG0780041172.01.T01:CDS 40.0%
ATCGTATTTCAGGCAATGGT+GGG - Chr7:87569358-87569377 None:intergenic 40.0%
CAAAACCGTTGAGAGAAACA+AGG + Chr7:87567782-87567801 MsG0780041172.01.T01:CDS 40.0%
CATGTGGTATCCCAAAACTA+AGG + Chr7:87568485-87568504 MsG0780041172.01.T01:CDS 40.0%
CTCCACCAAAATGATTTCGT+GGG + Chr7:87569392-87569411 MsG0780041172.01.T01:CDS 40.0%
CTTCTTGGATATGCATATCC+TGG + Chr7:87567747-87567766 MsG0780041172.01.T01:CDS 40.0%
GCTCAATCCAAGAGATTATC+CGG + Chr7:87568851-87568870 MsG0780041172.01.T01:CDS 40.0%
TGGCAAAATTGCACTGAGTT+GGG + Chr7:87568791-87568810 MsG0780041172.01.T01:CDS 40.0%
TGTTGATGTTATGTCGGTGT+CGG - Chr7:87568980-87568999 None:intergenic 40.0%
TGTTGCCAGTACTGTTACTA+AGG + Chr7:87569283-87569302 MsG0780041172.01.T01:intron 40.0%
TGTTTCTTGGCACAAACTCA+GGG - Chr7:87568071-87568090 None:intergenic 40.0%
TTGCAGATGTGGTTATTGGA+TGG + Chr7:87568330-87568349 MsG0780041172.01.T01:CDS 40.0%
TTGCCCTGTTCATATACATG+TGG + Chr7:87568469-87568488 MsG0780041172.01.T01:CDS 40.0%
TTTCAAGAGTGGAGAGTTAG+AGG + Chr7:87568746-87568765 MsG0780041172.01.T01:CDS 40.0%
! TATTGGCTATGAAGCACTAC+TGG - Chr7:87569067-87569086 None:intergenic 40.0%
!! TTCGTTTCTGGTGTCAATAC+TGG + Chr7:87567820-87567839 MsG0780041172.01.T01:CDS 40.0%
!! TTTTGAAGGTTGCCCATGTA+TGG + Chr7:87568435-87568454 MsG0780041172.01.T01:intron 40.0%
AAGAGTGGAGAGTTAGAGGA+TGG + Chr7:87568750-87568769 MsG0780041172.01.T01:CDS 45.0%
ACTGACACTTCGACATGTGT+TGG + Chr7:87568951-87568970 MsG0780041172.01.T01:intron 45.0%
AGAGTGGAGAGTTAGAGGAT+GGG + Chr7:87568751-87568770 MsG0780041172.01.T01:CDS 45.0%
CTGGCAAAATTGCACTGAGT+TGG + Chr7:87568790-87568809 MsG0780041172.01.T01:CDS 45.0%
GAGAGTTAGAGGATGGGATT+TGG + Chr7:87568757-87568776 MsG0780041172.01.T01:CDS 45.0%
GATCGTATTTCAGGCAATGG+TGG - Chr7:87569359-87569378 None:intergenic 45.0%
GCTGATCGTATTTCAGGCAA+TGG - Chr7:87569362-87569381 None:intergenic 45.0%
GGATTTGCCGGATAATCTCT+TGG - Chr7:87568861-87568880 None:intergenic 45.0%
GGCAAAATTGCACTGAGTTG+GGG + Chr7:87568792-87568811 MsG0780041172.01.T01:CDS 45.0%
GTTTCTGCAACTCACCTTCT+TGG - Chr7:87568885-87568904 None:intergenic 45.0%
TCGTATTTCAGGCAATGGTG+GGG - Chr7:87569357-87569376 None:intergenic 45.0%
! CTGTTTCTTGGCACAAACTC+AGG - Chr7:87568072-87568091 None:intergenic 45.0%
! GCTTCTGAAAAAGGTGCACA+TGG - Chr7:87568160-87568179 None:intergenic 45.0%
ATCCGGCAAATCCTCCAAGA+AGG + Chr7:87568868-87568887 MsG0780041172.01.T01:CDS 50.0%
CACCTTCTTGGAGGATTTGC+CGG - Chr7:87568873-87568892 None:intergenic 50.0%
TCTGCAACTCACCTTCTTGG+AGG - Chr7:87568882-87568901 None:intergenic 50.0%
! ATGTCGAAGTGTCAGTCACG+AGG - Chr7:87568947-87568966 None:intergenic 50.0%
! GCTATGAAGCACTACTGGAC+AGG - Chr7:87569062-87569081 None:intergenic 50.0%
! GGCCCACGAAATCATTTTGG+TGG - Chr7:87569397-87569416 None:intergenic 50.0%
! TGCGGCCCACGAAATCATTT+TGG - Chr7:87569400-87569419 None:intergenic 50.0%
! TGTTGCTTGATGTAGGGGAC+AGG + Chr7:87568659-87568678 MsG0780041172.01.T01:intron 50.0%
! AATGGTGGGGCACTAGGAAC+AGG - Chr7:87569344-87569363 None:intergenic 55.0%
! ACAGGTTGAGTCGTCGACAC+CGG - Chr7:87569044-87569063 None:intergenic 55.0%
! TCAGGCAATGGTGGGGCACT+AGG - Chr7:87569350-87569369 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 87567570 87569430 87567570 ID=MsG0780041172.01;Name=MsG0780041172.01
Chr7 mRNA 87567570 87569430 87567570 ID=MsG0780041172.01.T01;Parent=MsG0780041172.01;Name=MsG0780041172.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|6|0|191
Chr7 exon 87567570 87567601 87567570 ID=MsG0780041172.01.T01:exon:52204;Parent=MsG0780041172.01.T01
Chr7 exon 87567743 87567841 87567743 ID=MsG0780041172.01.T01:exon:52205;Parent=MsG0780041172.01.T01
Chr7 exon 87568295 87568351 87568295 ID=MsG0780041172.01.T01:exon:52206;Parent=MsG0780041172.01.T01
Chr7 exon 87568443 87568506 87568443 ID=MsG0780041172.01.T01:exon:52207;Parent=MsG0780041172.01.T01
Chr7 exon 87568674 87568889 87568674 ID=MsG0780041172.01.T01:exon:52208;Parent=MsG0780041172.01.T01
Chr7 exon 87569323 87569430 87569323 ID=MsG0780041172.01.T01:exon:52209;Parent=MsG0780041172.01.T01
Chr7 CDS 87567570 87567601 87567570 ID=MsG0780041172.01.T01:cds;Parent=MsG0780041172.01.T01
Chr7 CDS 87567743 87567841 87567743 ID=MsG0780041172.01.T01:cds;Parent=MsG0780041172.01.T01
Chr7 CDS 87568295 87568351 87568295 ID=MsG0780041172.01.T01:cds;Parent=MsG0780041172.01.T01
Chr7 CDS 87568443 87568506 87568443 ID=MsG0780041172.01.T01:cds;Parent=MsG0780041172.01.T01
Chr7 CDS 87568674 87568889 87568674 ID=MsG0780041172.01.T01:cds;Parent=MsG0780041172.01.T01
Chr7 CDS 87569323 87569430 87569323 ID=MsG0780041172.01.T01:cds;Parent=MsG0780041172.01.T01
Gene Sequence

>MsG0780041172.01.T01

ATGTTAGGGGATTTCATTACTCGTTGTCTTATATTGCTTCTTGGATATGCATATCCTGGATTTGAATGCTACAAAACCGTTGAGAGAAACAAGGTTGAAATGGATGAACTTCGTTTCTGGTGTCAATACTGGATCATTGTAGCTTTTTTCACAGTACTGGAAAAGTTTGCAGATGTGGTTATTGGATGGTTGCCCATGTATGGAGAATTGAAGCTTGCCCTGTTCATATACATGTGGTATCCCAAAACTAAGGGGACAGGATATGTTTATAATAAAGTGTTGAGACCATATGTATCTAAGAATGAAATTGACTTTGACAAGAAGTTTCAAGAGTGGAGAGTTAGAGGATGGGATTTGGCAATTTTTTACTGGCAAAATTGCACTGAGTTGGGGCAAACTGCATTTTTTCAAGTTATTGATCACTTGGCTGCTCAATCCAAGAGATTATCCGGCAAATCCTCCAAGAAGAAAAAAGATGGGATGAATCCTGTTCCTAGTGCCCCACCATTGCCTGAAATACGATCAGCATTGTTTGAACTCCACCAAAATGATTTCGTGGGCCGCAAGAAGAAGTAA

Protein sequence

>MsG0780041172.01.T01

MLGDFITRCLILLLGYAYPGFECYKTVERNKVEMDELRFWCQYWIIVAFFTVLEKFADVVIGWLPMYGELKLALFIYMWYPKTKGTGYVYNKVLRPYVSKNEIDFDKKFQEWRVRGWDLAIFYWQNCTELGQTAFFQVIDHLAAQSKRLSGKSSKKKKDGMNPVPSAPPLPEIRSALFELHQNDFVGRKKK*