AlfalfaGEDB | MsG0180001104.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001104.01

KEGG: -
GO: -
PFAM: PF14009

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001104.01.T01	XP_013466508.1	96.875	192	6	0	1	192	1	192	3.69E-130	374

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001104.01.T01	Q6NLC8	50	176	74	4	1	163	1	175	2.15E-45	152

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001104.01.T01	A0A072VEV4	96.875	192	6	0	1	192	1	192	1.76e-130	374

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048302.01	MsG0180001104.01	0.812007	5.263793e-51	3.308058e-48
MsG0180001104.01	MsG0280006884.01	0.802514	5.412321e-49	2.656257e-46
MsG0180001104.01	MsG0280007686.01	0.812306	4.530784e-51	2.870563e-48
MsG0180001104.01	MsG0280009241.01	0.812201	4.776795e-51	3.017844e-48
MsG0180001104.01	MsG0280009416.01	0.847588	1.051992e-59	1.878851e-56
MsG0180001104.01	MsG0280010296.01	0.842500	2.489880e-58	3.782629e-55
MsG0180001104.01	MsG0280010502.01	0.808708	2.712530e-50	1.561842e-47
MsG0180001104.01	MsG0280010785.01	0.811727	6.057795e-51	3.778601e-48
MsG0180001104.01	MsG0380013406.01	0.827587	1.440688e-54	1.396732e-51
MsG0180001104.01	MsG0380016008.01	0.807372	5.219870e-50	2.902353e-47
MsG0180001104.01	MsG0380016018.01	0.818148	2.280383e-52	1.693300e-49
MsG0180001104.01	MsG0480018256.01	0.804260	2.353186e-49	1.207711e-46
MsG0180001104.01	MsG0480018391.01	0.801215	1.000439e-48	4.750907e-46
MsG0180001104.01	MsG0480019107.01	0.811410	7.099048e-51	4.391245e-48
MsG0180001104.01	MsG0480020722.01	0.806304	8.776635e-50	4.747539e-47
MsG0180001104.01	MsG0480021705.01	0.819145	1.354339e-52	1.033703e-49
MsG0180001104.01	MsG0480023541.01	0.817042	4.047616e-52	2.915969e-49
MsG0180001104.01	MsG0580024035.01	0.829863	4.056972e-55	4.204703e-52
MsG0180001104.01	MsG0580024478.01	0.802795	4.735125e-49	2.340793e-46
MsG0180001104.01	MsG0580027641.01	0.807041	6.133664e-50	3.381295e-47
MsG0180001104.01	MsG0580028307.01	0.814035	1.891278e-51	1.255228e-48
MsG0180001104.01	MsG0580028924.01	0.858405	8.428498e-63	2.148751e-59
MsG0180001104.01	MsG0580029950.01	0.822219	2.663112e-53	2.215092e-50
MsG0180001104.01	MsG0680031655.01	0.803667	3.125599e-49	1.579879e-46
MsG0180001104.01	MsG0780037094.01	0.851932	6.429157e-61	1.321724e-57
MsG0180001104.01	MsG0780039430.01	0.827199	1.784515e-54	1.710932e-51
MsG0180001104.01	MsG0780041641.01	0.830827	2.358720e-55	2.513788e-52
MsG0180001104.01	MsG0880043261.01	0.809865	1.531648e-50	9.095136e-48
MsG0180001104.01	MsG0880043262.01	0.834675	2.612599e-56	3.122277e-53
MsG0180001104.01	MsG0880043921.01	0.821178	4.635373e-53	3.744156e-50
MsG0180001104.01	MsG0880045256.01	0.813144	2.969251e-51	1.923483e-48
MsG0180001104.01	MsG0880045790.01	0.805125	1.552471e-49	8.147754e-47
MsG0180001104.01	MsG0880046615.01	0.814963	1.178669e-51	8.021491e-49
MsG0180001104.01	MsG0880046762.01	0.807472	4.971284e-50	2.771467e-47
MsG0180001104.01	MsG0880047293.01	0.802712	4.926771e-49	2.430300e-46

PPI

Gene1	Gene2	Type
MsG0180001104.01	MsG0380015315.01	PPI
MsG0180001104.01	MsG0380015315.01	PPI
MsG0180001104.01	MsG0380015315.01	PPI
MsG0180001104.01	MsG0380015315.01	PPI
MsG0180001104.01	MsG0380015315.01	PPI
MsG0180001104.01	MsG0380015315.01	PPI
MsG0180001104.01	MsG0580028988.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001104.01.T01	MTR_1g030740	96.875	192	6	0	1	192	1	192	4.47e-134	374
MsG0180001104.01.T01	MTR_4g085100	70.857	175	43	4	1	170	1	172	5.57e-77	229
MsG0180001104.01.T01	MTR_5g079610	54.070	172	69	4	1	164	1	170	1.68e-55	175
MsG0180001104.01.T01	MTR_3g067730	49.746	197	84	4	1	182	1	197	1.11e-52	168

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001104.01.T01	AT2G01340	63.529	170	57	3	1	169	1	166	4.27e-64	197
MsG0180001104.01.T01	AT1G71015	57.576	165	64	3	1	163	1	161	7.53e-49	157
MsG0180001104.01.T01	AT1G71015	57.576	165	64	3	1	163	1	161	7.53e-49	157
MsG0180001104.01.T01	AT1G66480	50.000	176	74	4	1	163	1	175	2.19e-46	152
MsG0180001104.01.T01	AT5G37840	42.132	197	104	6	1	191	1	193	1.81e-39	134

Find 40 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAATAATGGGAAATAGTTT+AGG	0.332015	1:+16054433	None:intergenic
TCTGAGGAAGTGAAGCATTA+TGG	0.340674	1:+16054568	MsG0180001104.01.T01:CDS
TAATGATAAGAAAATGAAAA+AGG	0.345766	1:+16054939	MsG0180001104.01.T01:CDS
CCCACCTCGAAGTCGTTCAT+TGG	0.353620	1:-16054778	None:intergenic
TCTTGTAACTCGACTTTCCT+TGG	0.360185	1:-16054662	None:intergenic
AACTTCTCAACATCTGCCTT+TGG	0.375562	1:-16054847	None:intergenic
AACTCTTTGTGTGCTTCCAA+TGG	0.426840	1:-16054604	None:intergenic
GAAAGCCGGTGAAGTGCTTA+AGG	0.427598	1:+16054519	MsG0180001104.01.T01:CDS
GTTACAAGAAGGGTTCGTTC+GGG	0.451959	1:+16054676	MsG0180001104.01.T01:CDS
CTTGTAACTCGACTTTCCTT+GGG	0.453744	1:-16054661	None:intergenic
AGTTACAAGAAGGGTTCGTT+CGG	0.464996	1:+16054675	MsG0180001104.01.T01:CDS
AAGAAGAGTTGACGAATGGC+GGG	0.488902	1:+16054803	MsG0180001104.01.T01:CDS
AGCACTTCACCGGCTTTCAC+CGG	0.493553	1:-16054514	None:intergenic
GTGATCCTTAAGCACTTCAC+CGG	0.518764	1:-16054524	None:intergenic
ACAAAGGTGATGAAAATTGA+TGG	0.530975	1:+16054469	MsG0180001104.01.T01:CDS
TCATTGGTTTCATAATCGTA+AGG	0.535977	1:-16054762	None:intergenic
AGTTGACGAATGGCGGGGTG+AGG	0.542180	1:+16054809	MsG0180001104.01.T01:CDS
CTAAAGACACCGGTGAAAGC+CGG	0.542691	1:+16054505	MsG0180001104.01.T01:CDS
TCTTGTCTTGTTAGAATCTG+AGG	0.544333	1:+16054552	MsG0180001104.01.T01:CDS
TGGAAGGGTAGCAGAGGAGT+TGG	0.545103	1:+16054970	MsG0180001104.01.T01:CDS
GAAACCAATGAACGACTTCG+AGG	0.561915	1:+16054774	MsG0180001104.01.T01:CDS
AGGAAAGTCGAGTTACAAGA+AGG	0.564448	1:+16054665	MsG0180001104.01.T01:CDS
ATAATGGGAAATAGTTTAGG+TGG	0.567511	1:+16054436	None:intergenic
GAAGATTATGAGTCTTTGCA+TGG	0.572826	1:+16054906	MsG0180001104.01.T01:CDS
TGGAGTGAGAGCGAAGCCAT+TGG	0.574068	1:+16054588	MsG0180001104.01.T01:CDS
AAAGAAGAGTTGACGAATGG+CGG	0.576273	1:+16054802	MsG0180001104.01.T01:CDS
GATTCTAACAAGACAAGACC+AGG	0.591028	1:-16054547	None:intergenic
ACCAATGAACGACTTCGAGG+TGG	0.613366	1:+16054777	MsG0180001104.01.T01:CDS
GATGCAAAGTAGTAAAGATG+AGG	0.620365	1:+16054870	MsG0180001104.01.T01:CDS
AGAGTCTGTCTTTAGCTCGA+AGG	0.625951	1:+16054728	MsG0180001104.01.T01:CDS
CCAATGAACGACTTCGAGGT+GGG	0.626451	1:+16054778	MsG0180001104.01.T01:CDS
TTACAAGAAGGGTTCGTTCG+GGG	0.631852	1:+16054677	MsG0180001104.01.T01:CDS
ATGCATTGGAAGGGTAGCAG+AGG	0.643927	1:+16054964	MsG0180001104.01.T01:CDS
GGAAAGTCGAGTTACAAGAA+GGG	0.646808	1:+16054666	MsG0180001104.01.T01:CDS
GGAAAAGAAGAGTTGACGAA+TGG	0.648290	1:+16054799	MsG0180001104.01.T01:CDS
AGAAGAGTTGACGAATGGCG+GGG	0.660824	1:+16054804	MsG0180001104.01.T01:CDS
GTTGAAAATGAGACTTCCAA+AGG	0.662765	1:+16054831	MsG0180001104.01.T01:CDS
AACATTCAAGCTAAAGACAC+CGG	0.668856	1:+16054495	MsG0180001104.01.T01:CDS
ATTCTAACAAGACAAGACCA+GGG	0.767432	1:-16054546	None:intergenic
GAAGTGCTTAAGGATCACCC+TGG	0.784850	1:+16054529	MsG0180001104.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATGATAAGAAAATGAAAA+AGG	+	Chr1:16054939-16054958	MsG0180001104.01.T01:CDS	15.0%
!	AAATGAAAAAGGAAATGCAT+TGG	+	Chr1:16054950-16054969	MsG0180001104.01.T01:CDS	25.0%
!	TGAATACAAAAAAGCTTATG+AGG	+	Chr1:16054993-16055012	MsG0180001104.01.T01:CDS	25.0%
!!	AAAAAGACTCTATTTTCTTG+TGG	+	Chr1:16054633-16054652	MsG0180001104.01.T01:CDS	25.0%
!!	ACAAGAAAATAGAGTCTTTT+TGG	-	Chr1:16054634-16054653	None:intergenic	25.0%
	AAAAAGGAAATGCATTGGAA+GGG	+	Chr1:16054955-16054974	MsG0180001104.01.T01:CDS	30.0%
	ACAAAGGTGATGAAAATTGA+TGG	+	Chr1:16054469-16054488	MsG0180001104.01.T01:CDS	30.0%
	AGGTGGAAAAAAATCTACAA+AGG	+	Chr1:16054453-16054472	MsG0180001104.01.T01:CDS	30.0%
!!	TCATTGGTTTCATAATCGTA+AGG	-	Chr1:16054765-16054784	None:intergenic	30.0%
	AACATTCAAGCTAAAGACAC+CGG	+	Chr1:16054495-16054514	MsG0180001104.01.T01:CDS	35.0%
	ATTCTAACAAGACAAGACCA+GGG	-	Chr1:16054549-16054568	None:intergenic	35.0%
	GAAAAAGGAAATGCATTGGA+AGG	+	Chr1:16054954-16054973	MsG0180001104.01.T01:CDS	35.0%
	GAAGATTATGAGTCTTTGCA+TGG	+	Chr1:16054906-16054925	MsG0180001104.01.T01:CDS	35.0%
	GATGCAAAGTAGTAAAGATG+AGG	+	Chr1:16054870-16054889	MsG0180001104.01.T01:CDS	35.0%
	GTTGAAAATGAGACTTCCAA+AGG	+	Chr1:16054831-16054850	MsG0180001104.01.T01:CDS	35.0%
!	TCTTGTCTTGTTAGAATCTG+AGG	+	Chr1:16054552-16054571	MsG0180001104.01.T01:CDS	35.0%
	AAAGAAGAGTTGACGAATGG+CGG	+	Chr1:16054802-16054821	MsG0180001104.01.T01:CDS	40.0%
	AACTCTTTGTGTGCTTCCAA+TGG	-	Chr1:16054607-16054626	None:intergenic	40.0%
	AACTTCTCAACATCTGCCTT+TGG	-	Chr1:16054850-16054869	None:intergenic	40.0%
	AGGAAAGTCGAGTTACAAGA+AGG	+	Chr1:16054665-16054684	MsG0180001104.01.T01:CDS	40.0%
	AGTTACAAGAAGGGTTCGTT+CGG	+	Chr1:16054675-16054694	MsG0180001104.01.T01:CDS	40.0%
	CTTGTAACTCGACTTTCCTT+GGG	-	Chr1:16054664-16054683	None:intergenic	40.0%
	GATTCTAACAAGACAAGACC+AGG	-	Chr1:16054550-16054569	None:intergenic	40.0%
	GGAAAAGAAGAGTTGACGAA+TGG	+	Chr1:16054799-16054818	MsG0180001104.01.T01:CDS	40.0%
	GGAAAGTCGAGTTACAAGAA+GGG	+	Chr1:16054666-16054685	MsG0180001104.01.T01:CDS	40.0%
	TCTTGTAACTCGACTTTCCT+TGG	-	Chr1:16054665-16054684	None:intergenic	40.0%
!	TCTGAGGAAGTGAAGCATTA+TGG	+	Chr1:16054568-16054587	MsG0180001104.01.T01:CDS	40.0%
!	TTTTCTTGTGGAACTTCCCA+AGG	+	Chr1:16054645-16054664	MsG0180001104.01.T01:CDS	40.0%
	AAGAAGAGTTGACGAATGGC+GGG	+	Chr1:16054803-16054822	MsG0180001104.01.T01:CDS	45.0%
	GAAACCAATGAACGACTTCG+AGG	+	Chr1:16054774-16054793	MsG0180001104.01.T01:CDS	45.0%
	GTGATCCTTAAGCACTTCAC+CGG	-	Chr1:16054527-16054546	None:intergenic	45.0%
	GTTACAAGAAGGGTTCGTTC+GGG	+	Chr1:16054676-16054695	MsG0180001104.01.T01:CDS	45.0%
	TTACAAGAAGGGTTCGTTCG+GGG	+	Chr1:16054677-16054696	MsG0180001104.01.T01:CDS	45.0%
!	AGAGTCTGTCTTTAGCTCGA+AGG	+	Chr1:16054728-16054747	MsG0180001104.01.T01:CDS	45.0%
	ACCAATGAACGACTTCGAGG+TGG	+	Chr1:16054777-16054796	MsG0180001104.01.T01:CDS	50.0%
	AGAAGAGTTGACGAATGGCG+GGG	+	Chr1:16054804-16054823	MsG0180001104.01.T01:CDS	50.0%
	ATGCATTGGAAGGGTAGCAG+AGG	+	Chr1:16054964-16054983	MsG0180001104.01.T01:CDS	50.0%
	CCAATGAACGACTTCGAGGT+GGG	+	Chr1:16054778-16054797	MsG0180001104.01.T01:CDS	50.0%
	CTAAAGACACCGGTGAAAGC+CGG	+	Chr1:16054505-16054524	MsG0180001104.01.T01:CDS	50.0%
!	GAAAGCCGGTGAAGTGCTTA+AGG	+	Chr1:16054519-16054538	MsG0180001104.01.T01:CDS	50.0%
!	GAAGTGCTTAAGGATCACCC+TGG	+	Chr1:16054529-16054548	MsG0180001104.01.T01:CDS	50.0%
	CCCACCTCGAAGTCGTTCAT+TGG	-	Chr1:16054781-16054800	None:intergenic	55.0%
	TGGAAGGGTAGCAGAGGAGT+TGG	+	Chr1:16054970-16054989	MsG0180001104.01.T01:CDS	55.0%
	TGGAGTGAGAGCGAAGCCAT+TGG	+	Chr1:16054588-16054607	MsG0180001104.01.T01:CDS	55.0%
!!	AGCACTTCACCGGCTTTCAC+CGG	-	Chr1:16054517-16054536	None:intergenic	55.0%
!	AGTTGACGAATGGCGGGGTG+AGG	+	Chr1:16054809-16054828	MsG0180001104.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	16054439	16055020	16054439	ID=MsG0180001104.01;Name=MsG0180001104.01
Chr1	mRNA	16054439	16055020	16054439	ID=MsG0180001104.01.T01;Parent=MsG0180001104.01;Name=MsG0180001104.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|193
Chr1	exon	16054439	16055020	16054439	ID=MsG0180001104.01.T01:exon:31191;Parent=MsG0180001104.01.T01
Chr1	CDS	16054439	16055020	16054439	ID=MsG0180001104.01.T01:cds;Parent=MsG0180001104.01.T01

Gene Sequence

>MsG0180001104.01.T01

ATGGGAAATAGTTTAGGTGGAAAAAAATCTACAAAGGTGATGAAAATTGATGGTGAAACATTCAAGCTAAAGACACCGGTGAAAGCCGGTGAAGTGCTTAAGGATCACCCTGGTCTTGTCTTGTTAGAATCTGAGGAAGTGAAGCATTATGGAGTGAGAGCGAAGCCATTGGAAGCACACAAAGAGTTGAAGCCAAAAAGACTCTATTTTCTTGTGGAACTTCCCAAGGAAAGTCGAGTTACAAGAAGGGTTCGTTCGGGGATTAATATGAGTGCTAAAGATAGACTCGAGAGTCTGTCTTTAGCTCGAAGGTCAGCTTCTGACCTTACGATTATGAAACCAATGAACGACTTCGAGGTGGGAAAAGAAGAGTTGACGAATGGCGGGGTGAGGTTGAAAATGAGACTTCCAAAGGCAGATGTTGAGAAGTTGATGCAAAGTAGTAAAGATGAGGCTGATGCTGCTGAGAAGATTATGAGTCTTTGCATGGCTAATGATAGTAATGATAAGAAAATGAAAAAGGAAATGCATTGGAAGGGTAGCAGAGGAGTTGGTGAATACAAAAAAGCTTATGAGGTATGA

Protein sequence

>MsG0180001104.01.T01

MGNSLGGKKSTKVMKIDGETFKLKTPVKAGEVLKDHPGLVLLESEEVKHYGVRAKPLEAHKELKPKRLYFLVELPKESRVTRRVRSGINMSAKDRLESLSLARRSASDLTIMKPMNDFEVGKEELTNGGVRLKMRLPKADVEKLMQSSKDEADAAEKIMSLCMANDSNDKKMKKEMHWKGSRGVGEYKKAYEV*