AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041641.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041641.01.T01 MTR_7g114970 84.663 163 6 1 8 170 58 201 2.40e-96 277
MsG0780041641.01.T01 MTR_7g114970 84.663 163 6 1 8 170 106 249 1.09e-95 277
MsG0780041641.01.T01 MTR_7g114970 84.663 163 6 1 8 170 200 343 1.16e-94 278
MsG0780041641.01.T01 MTR_1g102750 54.321 162 54 2 8 169 199 340 3.47e-57 182
MsG0780041641.01.T01 MTR_7g114980 55.556 162 52 2 8 169 208 349 2.39e-56 181
MsG0780041641.01.T01 MTR_7g114970 79.048 105 3 1 8 112 200 285 3.92e-52 168
MsG0780041641.01.T01 MTR_7g114970 78.846 104 3 1 8 111 200 284 3.11e-51 166
MsG0780041641.01.T01 MTR_7g114990 51.235 162 57 3 8 169 206 345 1.34e-50 166
MsG0780041641.01.T01 MTR_7g115010 53.049 164 55 3 8 169 187 330 1.26e-49 162
MsG0780041641.01.T01 MTR_1g102740 58.716 109 44 1 61 169 1 108 1.44e-43 140
MsG0780041641.01.T01 MTR_7g021850 44.720 161 63 5 8 167 196 331 1.38e-35 126
MsG0780041641.01.T01 MTR_1g102750 54.206 107 29 2 8 114 199 285 1.42e-33 120
MsG0780041641.01.T01 MTR_7g114980 57.143 105 25 2 8 112 208 292 1.60e-32 117
MsG0780041641.01.T01 MTR_7g021680 42.331 163 69 6 6 167 193 331 3.02e-32 118
MsG0780041641.01.T01 MTR_7g021820 42.331 163 69 6 6 167 193 331 3.02e-32 118
MsG0780041641.01.T01 MTR_7g021825 45.385 130 50 4 6 134 51 160 1.58e-29 106
MsG0780041641.01.T01 MTR_7g067855 38.926 149 55 3 56 169 8 155 8.71e-28 102
MsG0780041641.01.T01 MTR_7g021670 44.444 108 49 3 63 167 146 245 3.43e-24 95.1
MsG0780041641.01.T01 MTR_6g035180 46.552 58 31 0 112 169 7 64 1.04e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041641.01.T01 AT1G60680 42.857 161 69 4 8 167 200 338 1.69e-35 126
MsG0780041641.01.T01 AT1G60710 44.172 163 68 4 6 167 197 337 1.83e-35 126
MsG0780041641.01.T01 AT1G60690 42.331 163 71 4 6 167 197 337 4.68e-35 125
MsG0780041641.01.T01 AT1G60730 41.104 163 73 4 6 167 197 337 1.73e-33 121
MsG0780041641.01.T01 AT1G60730 41.104 163 73 4 6 167 217 357 2.02e-33 121
MsG0780041641.01.T01 AT1G10810 41.104 163 73 4 6 167 145 285 5.38e-32 116
MsG0780041641.01.T01 AT1G10810 41.615 161 71 4 8 167 199 337 1.26e-31 116
MsG0780041641.01.T01 AT1G60750 39.506 162 60 3 6 167 199 322 2.00e-30 113

Find 40 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATAAAACCGATTGTTAT+TGG 0.178666 7:-93357633 MsG0780041641.01.T01:three_prime_UTR
GACTGTCATCAGAAACAAAT+TGG 0.209028 7:+93358500 None:intergenic
TTTGAGACTCAAACTAGTTC+TGG 0.313286 7:-93357689 MsG0780041641.01.T01:three_prime_UTR
CACATTAATTATAAGTAAAC+TGG 0.315738 7:+93357538 None:intergenic
TGTTTGGTGACAACTCTTAC+AGG 0.321536 7:-93358632 MsG0780041641.01.T01:CDS
GTACTAATATATTCCTTGTT+TGG 0.328996 7:-93357505 MsG0780041641.01.T01:three_prime_UTR
TCCCCTGACACCTCAGATAT+TGG 0.333520 7:+93357802 None:intergenic
TCGAAATGACTTCGGTTGTT+TGG 0.357221 7:-93358648 None:intergenic
GTAACCTTGGTTGCATTGCC+TGG 0.401419 7:+93358259 None:intergenic
CGATTGTTATTGGTGAAATA+CGG 0.424235 7:-93357623 MsG0780041641.01.T01:three_prime_UTR
ACTCAACAAAGATGATTTGA+AGG 0.433839 7:-93357842 MsG0780041641.01.T01:CDS
CTTCACAACTTGCTCTTGCA+TGG 0.444626 7:-93358158 MsG0780041641.01.T01:CDS
CCTTACAGTCCCCTTGGCCG+TGG 0.464143 7:-93358588 MsG0780041641.01.T01:CDS
TTATCATCGGATGGAAAAGT+TGG 0.465236 7:-93358205 MsG0780041641.01.T01:CDS
GTGTCAGGGGATCGGACAAC+TGG 0.468035 7:-93357790 MsG0780041641.01.T01:CDS
CGTATTTCACCAATAACAAT+CGG 0.468386 7:+93357624 None:intergenic
GTGCCAATATCTGAGGTGTC+AGG 0.468582 7:-93357805 MsG0780041641.01.T01:CDS
GACAACTCTTACAGGGAACT+TGG 0.479074 7:-93358624 MsG0780041641.01.T01:CDS
GCAATGCAACCAAGGTTACA+AGG 0.480835 7:-93358255 MsG0780041641.01.T01:CDS
GGAAAGTTACTCACCAGGGA+TGG 0.508635 7:+93358107 None:intergenic
CCACGGCCAAGGGGACTGTA+AGG 0.509324 7:+93358588 None:intergenic
GACGATGTAGTGCCCATCCC+TGG 0.510769 7:-93358120 MsG0780041641.01.T01:intron
TCTTACAGGGAACTTGGCAT+TGG 0.523565 7:-93358618 MsG0780041641.01.T01:CDS
ACTGTCATCAGAAACAAATT+GGG 0.524263 7:+93358501 None:intergenic
TGCCAATATCTGAGGTGTCA+GGG 0.538436 7:-93357804 MsG0780041641.01.T01:CDS
CTTGCATGGATTCTTCATCA+AGG 0.547230 7:-93358144 MsG0780041641.01.T01:CDS
TATCTGAGGTGTCAGGGGAT+CGG 0.577450 7:-93357798 MsG0780041641.01.T01:CDS
TCGGATGGAAAAGTTGGCAC+AGG 0.579387 7:-93358199 MsG0780041641.01.T01:CDS
ATAGTGCCTTACAGTCCCCT+TGG 0.580787 7:-93358594 MsG0780041641.01.T01:CDS
GCCAATATCTGAGGTGTCAG+GGG 0.582430 7:-93357803 MsG0780041641.01.T01:CDS
TTCACCAGGCAATGCAACCA+AGG 0.582900 7:-93358263 MsG0780041641.01.T01:CDS
GAAAGTTACTCACCAGGGAT+GGG 0.588008 7:+93358108 None:intergenic
TTCGCAGCTCTCTCCAAACA+AGG 0.592827 7:+93357492 None:intergenic
CAATGCAACCAAGGTTACAA+GGG 0.603737 7:-93358254 MsG0780041641.01.T01:CDS
TTAATCACATAAACATAACA+TGG 0.615428 7:+93357562 None:intergenic
CATACCAGAAAACTGTCTGC+AGG 0.620973 7:+93358531 None:intergenic
GTTTGGTGACAACTCTTACA+GGG 0.623722 7:-93358631 MsG0780041641.01.T01:CDS
AAGTTGGCACAGGAGAAGCA+TGG 0.666692 7:-93358189 MsG0780041641.01.T01:CDS
AATGCAACCAAGGTTACAAG+GGG 0.675322 7:-93358253 MsG0780041641.01.T01:CDS
AGATGCTGTGCCAATATCTG+AGG 0.705973 7:-93357812 MsG0780041641.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTCAAATAATTTCATC+TGG + Chr7:93358126-93358145 None:intergenic 15.0%
!! ATTGTTTATATTCAAACATT+TGG + Chr7:93357704-93357723 None:intergenic 15.0%
!! AACAAAAGAAATCATAATTG+AGG + Chr7:93358156-93358175 None:intergenic 20.0%
!! CACATTAATTATAAGTAAAC+TGG + Chr7:93358573-93358592 None:intergenic 20.0%
!! TTAATCACATAAACATAACA+TGG + Chr7:93358549-93358568 None:intergenic 20.0%
! AAACATTTATGAGACTGTTT+AGG + Chr7:93357755-93357774 None:intergenic 25.0%
! GTACTAATATATTCCTTGTT+TGG - Chr7:93358603-93358622 MsG0780041641.01.T01:CDS 25.0%
! TGAACATTCAGATTATACAA+GGG + Chr7:93358068-93358087 None:intergenic 25.0%
! TGAATAAAACCGATTGTTAT+TGG - Chr7:93358475-93358494 MsG0780041641.01.T01:intron 25.0%
! TTGAACATTCAGATTATACA+AGG + Chr7:93358069-93358088 None:intergenic 25.0%
! TTGTATAATCTGAATGTTCA+AGG - Chr7:93358068-93358087 MsG0780041641.01.T01:intron 25.0%
!! AGAACAAGATGTTTTATCAT+CGG - Chr7:93357890-93357909 MsG0780041641.01.T01:CDS 25.0%
!! GTTCATTTGAGTTTATTTAC+TGG + Chr7:93357781-93357800 None:intergenic 25.0%
!!! AACAATCGGTTTTATTCATA+TGG + Chr7:93358473-93358492 None:intergenic 25.0%
!!! ACAATCGGTTTTATTCATAT+GGG + Chr7:93358472-93358491 None:intergenic 25.0%
ACTCAACAAAGATGATTTGA+AGG - Chr7:93358266-93358285 MsG0780041641.01.T01:CDS 30.0%
ACTGTCATCAGAAACAAATT+GGG + Chr7:93357610-93357629 None:intergenic 30.0%
ATAATCTGAATGTTCAAGGA+AGG - Chr7:93358072-93358091 MsG0780041641.01.T01:intron 30.0%
CGTATTTCACCAATAACAAT+CGG + Chr7:93358487-93358506 None:intergenic 30.0%
TAATCTGAATGTTCAAGGAA+GGG - Chr7:93358073-93358092 MsG0780041641.01.T01:intron 30.0%
! CGATTGTTATTGGTGAAATA+CGG - Chr7:93358485-93358504 MsG0780041641.01.T01:intron 30.0%
AGTTTGAGTCTCAAAAACTG+AGG + Chr7:93358414-93358433 None:intergenic 35.0%
ATAATGAACAAGGACCTGTT+TGG + Chr7:93357808-93357827 None:intergenic 35.0%
ATGCTGGTGAATAATGAACA+AGG + Chr7:93357818-93357837 None:intergenic 35.0%
CAAAAACTGAGGTACAATAC+TGG + Chr7:93358403-93358422 None:intergenic 35.0%
GACTGTCATCAGAAACAAAT+TGG + Chr7:93357611-93357630 None:intergenic 35.0%
TCAAATGAACCAATCCAAAC+AGG - Chr7:93357791-93357810 MsG0780041641.01.T01:CDS 35.0%
TTATCATCGGATGGAAAAGT+TGG - Chr7:93357903-93357922 MsG0780041641.01.T01:intron 35.0%
! CAAGATGTTTTATCATCGGA+TGG - Chr7:93357894-93357913 MsG0780041641.01.T01:CDS 35.0%
! GCATCAAACTATGCTTTTCT+TGG + Chr7:93358381-93358400 None:intergenic 35.0%
! GTGCTTTTGTTCAATGTTCA+TGG - Chr7:93358340-93358359 MsG0780041641.01.T01:intron 35.0%
! TTTGAGACTCAAACTAGTTC+TGG - Chr7:93358419-93358438 MsG0780041641.01.T01:intron 35.0%
AAAGTGGAAAGTTACTCACC+AGG + Chr7:93358009-93358028 None:intergenic 40.0%
AAGTGGAAAGTTACTCACCA+GGG + Chr7:93358008-93358027 None:intergenic 40.0%
AATGCAACCAAGGTTACAAG+GGG - Chr7:93357855-93357874 MsG0780041641.01.T01:CDS 40.0%
CAATGCAACCAAGGTTACAA+GGG - Chr7:93357854-93357873 MsG0780041641.01.T01:CDS 40.0%
CTCAACAAGTTAGGCATCTA+TGG - Chr7:93358034-93358053 MsG0780041641.01.T01:intron 40.0%
CTTGCATGGATTCTTCATCA+AGG - Chr7:93357964-93357983 MsG0780041641.01.T01:intron 40.0%
CTTTGTGACCTCAACAAGTT+AGG - Chr7:93358025-93358044 MsG0780041641.01.T01:intron 40.0%
TTGTTCCTACAACACATGAC+AGG + Chr7:93358200-93358219 None:intergenic 40.0%
! AAGTTTTCCCCTTGTAACCT+TGG + Chr7:93357865-93357884 None:intergenic 40.0%
! ATGAGACTGTTTAGGACTCT+AGG + Chr7:93357747-93357766 None:intergenic 40.0%
! GTTTGGTGACAACTCTTACA+GGG - Chr7:93357477-93357496 MsG0780041641.01.T01:three_prime_UTR 40.0%
! TGTTTGGTGACAACTCTTAC+AGG - Chr7:93357476-93357495 MsG0780041641.01.T01:three_prime_UTR 40.0%
!! AACTTGTTGAGGTCACAAAG+TGG + Chr7:93358025-93358044 None:intergenic 40.0%
!! ACAGTTTTCTGGTATGGACT+TGG - Chr7:93357584-93357603 MsG0780041641.01.T01:three_prime_UTR 40.0%
!! CATAGATGCCTAACTTGTTG+AGG + Chr7:93358036-93358055 None:intergenic 40.0%
AGATGCTGTGCCAATATCTG+AGG - Chr7:93358296-93358315 MsG0780041641.01.T01:intron 45.0%
CATACCAGAAAACTGTCTGC+AGG + Chr7:93357580-93357599 None:intergenic 45.0%
CCTGGTGAAAAAAGAGATGC+TGG + Chr7:93357834-93357853 None:intergenic 45.0%
CCTTGCCTCCAAAAAATCCA+CGG + Chr7:93357540-93357559 None:intergenic 45.0%
GAAAGTTACTCACCAGGGAT+GGG + Chr7:93358003-93358022 None:intergenic 45.0%
GAACAAGGACCTGTTTGGAT+TGG + Chr7:93357803-93357822 None:intergenic 45.0%
GACAACTCTTACAGGGAACT+TGG - Chr7:93357484-93357503 MsG0780041641.01.T01:three_prime_UTR 45.0%
GCAATGCAACCAAGGTTACA+AGG - Chr7:93357853-93357872 MsG0780041641.01.T01:CDS 45.0%
TCCAAAAAATCCACGGCCAA+GGG + Chr7:93357533-93357552 None:intergenic 45.0%
TCTTACAGGGAACTTGGCAT+TGG - Chr7:93357490-93357509 MsG0780041641.01.T01:three_prime_UTR 45.0%
TGCAACCTGTCATGTGTTGT+AGG - Chr7:93358192-93358211 MsG0780041641.01.T01:CDS 45.0%
! CTTCACAACTTGCTCTTGCA+TGG - Chr7:93357950-93357969 MsG0780041641.01.T01:intron 45.0%
! TGCCAATATCTGAGGTGTCA+GGG - Chr7:93358304-93358323 MsG0780041641.01.T01:intron 45.0%
! TGTACCTGCAGACAGTTTTC+TGG - Chr7:93357573-93357592 MsG0780041641.01.T01:three_prime_UTR 45.0%
!! CTGCAGACAGTTTTCTGGTA+TGG - Chr7:93357578-93357597 MsG0780041641.01.T01:three_prime_UTR 45.0%
!!! CCAGCATCTCTTTTTTCACC+AGG - Chr7:93357831-93357850 MsG0780041641.01.T01:CDS 45.0%
AAGTTGGCACAGGAGAAGCA+TGG - Chr7:93357919-93357938 MsG0780041641.01.T01:intron 50.0%
ATAGTGCCTTACAGTCCCCT+TGG - Chr7:93357514-93357533 MsG0780041641.01.T01:three_prime_UTR 50.0%
CCAAAAAATCCACGGCCAAG+GGG + Chr7:93357532-93357551 None:intergenic 50.0%
CTCCAAAAAATCCACGGCCA+AGG + Chr7:93357534-93357553 None:intergenic 50.0%
GGAAAGTTACTCACCAGGGA+TGG + Chr7:93358004-93358023 None:intergenic 50.0%
GTAACCTTGGTTGCATTGCC+TGG + Chr7:93357852-93357871 None:intergenic 50.0%
TCCCCTGACACCTCAGATAT+TGG + Chr7:93358309-93358328 None:intergenic 50.0%
TCGGATGGAAAAGTTGGCAC+AGG - Chr7:93357909-93357928 MsG0780041641.01.T01:intron 50.0%
TTCACCAGGCAATGCAACCA+AGG - Chr7:93357845-93357864 MsG0780041641.01.T01:CDS 50.0%
TTCGCAGCTCTCTCCAAACA+AGG + Chr7:93358619-93358638 None:intergenic 50.0%
! GCCAATATCTGAGGTGTCAG+GGG - Chr7:93358305-93358324 MsG0780041641.01.T01:intron 50.0%
! GTGCCAATATCTGAGGTGTC+AGG - Chr7:93358303-93358322 MsG0780041641.01.T01:intron 50.0%
! TATCTGAGGTGTCAGGGGAT+CGG - Chr7:93358310-93358329 MsG0780041641.01.T01:intron 50.0%
!!! CCGTGGATTTTTTGGAGGCA+AGG - Chr7:93357537-93357556 MsG0780041641.01.T01:three_prime_UTR 50.0%
!!! CTTGGCCGTGGATTTTTTGG+AGG - Chr7:93357532-93357551 MsG0780041641.01.T01:three_prime_UTR 50.0%
!!! CCCCTTGGCCGTGGATTTTT+TGG - Chr7:93357529-93357548 MsG0780041641.01.T01:three_prime_UTR 55.0%
GACGATGTAGTGCCCATCCC+TGG - Chr7:93357988-93358007 MsG0780041641.01.T01:intron 60.0%
GTGTCAGGGGATCGGACAAC+TGG - Chr7:93358318-93358337 MsG0780041641.01.T01:intron 60.0%
CCACGGCCAAGGGGACTGTA+AGG + Chr7:93357523-93357542 None:intergenic 65.0%
CCTTACAGTCCCCTTGGCCG+TGG - Chr7:93357520-93357539 MsG0780041641.01.T01:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 93357475 93358655 93357475 ID=MsG0780041641.01;Name=MsG0780041641.01
Chr7 mRNA 93357475 93358655 93357475 ID=MsG0780041641.01.T01;Parent=MsG0780041641.01;Name=MsG0780041641.01.T01;_AED=0.47;_eAED=0.50;_QI=0|0.5|0.33|1|0.5|0.33|3|263|170
Chr7 exon 93357475 93357916 93357475 ID=MsG0780041641.01.T01:exon:22105;Parent=MsG0780041641.01.T01
Chr7 exon 93358121 93358295 93358121 ID=MsG0780041641.01.T01:exon:22104;Parent=MsG0780041641.01.T01
Chr7 exon 93358497 93358655 93358497 ID=MsG0780041641.01.T01:exon:22103;Parent=MsG0780041641.01.T01
Chr7 CDS 93358497 93358655 93358497 ID=MsG0780041641.01.T01:cds;Parent=MsG0780041641.01.T01
Chr7 CDS 93358121 93358295 93358121 ID=MsG0780041641.01.T01:cds;Parent=MsG0780041641.01.T01
Chr7 CDS 93357738 93357916 93357738 ID=MsG0780041641.01.T01:cds;Parent=MsG0780041641.01.T01
Chr7 three_prime_UTR 93357475 93357737 93357475 ID=MsG0780041641.01.T01:three_prime_utr;Parent=MsG0780041641.01.T01
Gene Sequence

>MsG0780041641.01.T01

TTGTTTGGTGACAACTCTTACAGGGAACTTGGCATTGGAATAGTGCCTTACAGTCCCCTTGGCCGTGGATTTTTTGGAGGCAAGGCTATTACAGAAAGTGTACCTGCAGACAGTTTTCTGGTATGGACTTGGCCCAATTTGTTTCTGATGACAGTCATTCATCTCTTTTTTCACCAGGCAATGCAACCAAGGTTACAAGGGGAAAACTTTGACAAGAACAAGATGTTTTATCATCGGATGGAAAAGTTGGCACAGGAGAAGCATGGATGTACATCTTCACAACTTGCTCTTGCATGGATTCTTCATCAAGGAGACGATGTAGTGCCCATCCCTGGAACAACTAAGATAAAAAATCTTGAAAGTAATATCAGTTCGTTTAAAGTGAAACTCAACAAAGATGATTTGAAGGAGATTGAAGATGCTGTGCCAATATCTGAGGTGTCAGGGGATCGGACAACTGGTGCTTTTGTTCAATGTTCATGGAAGTTTGCTAATACTCCAAGAAAAGCATAG

Protein sequence

>MsG0780041641.01.T01

LFGDNSYRELGIGIVPYSPLGRGFFGGKAITESVPADSFLVWTWPNLFLMTVIHLFFHQAMQPRLQGENFDKNKMFYHRMEKLAQEKHGCTSSQLALAWILHQGDDVVPIPGTTKIKNLESNISSFKVKLNKDDLKEIEDAVPISEVSGDRTTGAFVQCSWKFANTPRKA*