AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004312.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004312.01.T01 MTR_1g076850 98.466 326 5 0 1 326 1 326 0.0 659
MsG0180004312.01.T01 MTR_2g098950 67.089 316 104 0 8 323 14 329 3.52e-160 451
MsG0180004312.01.T01 MTR_4g067310 66.562 317 106 0 8 324 15 331 3.85e-157 443
MsG0180004312.01.T01 MTR_6g089480 64.401 309 108 2 15 321 1 309 6.74e-150 424
MsG0180004312.01.T01 MTR_1g101950 63.492 315 113 2 8 320 23 337 3.81e-149 423
MsG0180004312.01.T01 MTR_5g079460 66.026 312 105 1 5 315 64 375 9.34e-146 416
MsG0180004312.01.T01 MTR_6g089480 65.809 272 91 2 15 284 1 272 2.42e-132 378
MsG0180004312.01.T01 MTR_6g089480 66.296 270 89 2 15 282 1 270 3.63e-132 377
MsG0180004312.01.T01 MTR_7g075860 58.466 313 126 3 5 313 38 350 2.88e-130 376
MsG0180004312.01.T01 MTR_1g069490 49.841 315 130 3 8 320 110 398 5.41e-103 308
MsG0180004312.01.T01 MTR_1g057810 50.637 314 143 4 8 317 253 558 9.11e-103 313
MsG0180004312.01.T01 MTR_1g069745 50.865 289 128 4 34 320 9 285 4.53e-99 294
MsG0180004312.01.T01 MTR_1g105150 47.452 314 139 5 8 317 253 544 2.04e-90 280
MsG0180004312.01.T01 MTR_1g054730 45.055 273 121 2 48 320 144 387 2.99e-74 234
MsG0180004312.01.T01 MTR_5g044990 31.897 348 198 5 7 318 209 553 4.73e-53 183
MsG0180004312.01.T01 MTR_4g019165 35.220 318 173 4 34 318 133 450 1.21e-52 180
MsG0180004312.01.T01 MTR_0046s0180 52.427 103 49 0 218 320 7 109 7.94e-35 123
MsG0180004312.01.T01 MTR_1g070375 24.646 353 224 9 3 324 70 411 1.89e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004312.01.T01 AT4G10260 78.233 317 69 0 7 323 5 321 0.0 525
MsG0180004312.01.T01 AT3G59480 67.188 320 105 0 2 321 4 323 9.69e-163 457
MsG0180004312.01.T01 AT1G06020 67.610 318 103 0 8 325 10 327 5.61e-161 453
MsG0180004312.01.T01 AT1G06030 67.500 320 104 0 2 321 5 324 4.39e-160 450
MsG0180004312.01.T01 AT2G31390 67.197 314 103 0 8 321 9 322 1.30e-159 449
MsG0180004312.01.T01 AT1G66430 63.950 319 114 1 2 319 59 377 4.41e-152 432
MsG0180004312.01.T01 AT5G51830 63.863 321 114 2 8 326 23 343 5.84e-151 427
MsG0180004312.01.T01 AT5G51830 63.863 321 114 2 8 326 23 343 5.84e-151 427
MsG0180004312.01.T01 AT3G54090 34.072 361 190 7 7 320 102 461 7.85e-57 191
MsG0180004312.01.T01 AT1G50390 52.663 169 51 1 153 321 1 140 2.01e-53 172
MsG0180004312.01.T01 AT1G69200 31.307 329 190 4 7 300 205 532 6.37e-47 167
MsG0180004312.01.T01 AT1G06730 24.917 301 205 6 40 327 132 424 3.12e-12 67.8
MsG0180004312.01.T01 AT1G17160 23.973 292 192 7 39 321 105 375 5.21e-11 63.5

Find 80 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAAAGTCTCTCTCAATTT+TGG 0.120131 1:-76347005 None:intergenic
TGTCTCACAAATATAGTTTA+AGG 0.131310 1:-76344719 None:intergenic
ACCTTCTCTAGCAGCTTTCA+TGG 0.275867 1:-76345892 None:intergenic
TTGTATGAAAGTCTTATTAA+AGG 0.288285 1:-76347203 None:intergenic
GGCAGCATTGCCACCAAGTT+TGG 0.291331 1:-76344956 None:intergenic
AAGCATACCTCAAATATAGA+AGG 0.345785 1:-76346851 None:intergenic
AATACAAAGATATTCCATTA+TGG 0.367235 1:+76345822 MsG0180004312.01.T01:CDS
ACAGCACAAGCCACATTGGC+AGG 0.372362 1:-76344931 None:intergenic
TCTGCTGAGGCTGCTAGGTC+TGG 0.380493 1:+76345963 MsG0180004312.01.T01:CDS
AAGAAGCTGTTATGTCTCTA+TGG 0.387207 1:+76346213 MsG0180004312.01.T01:CDS
ACATTGGCAGGGGCACCGCC+AGG 0.390542 1:-76344919 None:intergenic
CAAATGTCAGGCTTCCTTTG+TGG 0.390789 1:+76345937 MsG0180004312.01.T01:CDS
TAAGACTAATGGATCCATAA+TGG 0.395043 1:-76345836 None:intergenic
ATTTCAAAGGAAAGTTTAGT+TGG 0.395477 1:-76347129 None:intergenic
CAGGTAGGGGATGATGAGTT+TGG 0.399164 1:+76345609 MsG0180004312.01.T01:intron
TGGTTCTGAAATAAGACTAA+TGG 0.406052 1:-76345847 None:intergenic
AAGAGACCCTTCTATATTTG+AGG 0.407481 1:+76346844 MsG0180004312.01.T01:CDS
GGTCTGGAATCAAAAGCATT+TGG 0.412730 1:+76345979 MsG0180004312.01.T01:CDS
ACCATGTAGATCTGCTCATA+TGG 0.422423 1:+76345866 MsG0180004312.01.T01:CDS
AAGCCTGACATTTGGATCAT+AGG 0.423973 1:-76345928 None:intergenic
TTTGTGACATTGAGGAAAGA+TGG 0.427613 1:+76345717 MsG0180004312.01.T01:CDS
GCTTGTGCTGTGTCCAAACT+TGG 0.436119 1:+76344943 MsG0180004312.01.T01:CDS
ATAACAGCTTCTTCATTCTC+AGG 0.444898 1:-76346203 None:intergenic
CACAAAGGAAGCCTGACATT+TGG 0.445582 1:-76345936 None:intergenic
AGTTTAGTTGGCTTTAGAGC+TGG 0.453062 1:-76347117 None:intergenic
GTGAAGGCAATTGACACAAC+TGG 0.456721 1:+76346779 MsG0180004312.01.T01:CDS
AGACACATCTGGTGTATCCT+TGG 0.457720 1:+76344870 MsG0180004312.01.T01:CDS
TTCTGAGCCTCTTCAGCAGT+TGG 0.458744 1:-76347086 None:intergenic
TCCTTTGTGGCCTTCTGCTG+AGG 0.474259 1:+76345950 MsG0180004312.01.T01:CDS
AAGAAGAAGAACAAGATGTC+AGG 0.478396 1:+76344793 MsG0180004312.01.T01:exon
GATCCATTGGTGATATCATT+CGG 0.485484 1:+76344823 MsG0180004312.01.T01:CDS
ATTGAGGAAAGATGGAGAGA+GGG 0.488808 1:+76345725 MsG0180004312.01.T01:CDS
ATTGGCAGGGGCACCGCCAG+GGG 0.492610 1:-76344917 None:intergenic
GGCCTTCTGCTGAGGCTGCT+AGG 0.493211 1:+76345958 MsG0180004312.01.T01:CDS
TGCACTTCCAACTGCTGAAG+AGG 0.495831 1:+76347079 MsG0180004312.01.T01:CDS
CATTGGCAGGGGCACCGCCA+GGG 0.499364 1:-76344918 None:intergenic
TCTTCAGCAGTTGGAAGTGC+AGG 0.503583 1:-76347077 None:intergenic
ATTAAGAACCTTGCCGATGA+AGG 0.504091 1:-76344977 None:intergenic
GACCTAGCAGCCTCAGCAGA+AGG 0.506010 1:-76345960 None:intergenic
AAGGATACACCAGATGTGTC+TGG 0.508328 1:-76344868 None:intergenic
AAGCTGCTTATTATCACTGA+TGG 0.515902 1:+76346248 MsG0180004312.01.T01:CDS
TGTGCTGTGTCCAAACTTGG+TGG 0.518945 1:+76344946 MsG0180004312.01.T01:CDS
CACAGAGGCAAGAACTGCAT+TGG 0.524471 1:+76345692 MsG0180004312.01.T01:CDS
AGCAGTTGGAAGTGCAGGAA+TGG 0.528605 1:-76347072 None:intergenic
TTGACATTTGCAAATGCTTG+TGG 0.529294 1:+76347023 MsG0180004312.01.T01:CDS
TGTCCTATGATCCAAATGTC+AGG 0.532074 1:+76345925 MsG0180004312.01.T01:CDS
GTCAGGCTCAAAAGATCCAT+TGG 0.534889 1:+76344810 MsG0180004312.01.T01:CDS
AATCAAAAGCATTTGGAATG+AGG 0.534914 1:+76345986 MsG0180004312.01.T01:CDS
CATTGAGGAAAGATGGAGAG+AGG 0.536347 1:+76345724 MsG0180004312.01.T01:CDS
TATTATCACTGATGGAGAGA+AGG 0.541140 1:+76346256 MsG0180004312.01.T01:CDS
GGTGGCAATGCTGCCTTCAT+CGG 0.543040 1:+76344964 MsG0180004312.01.T01:CDS
TTCAGTAACATATCAGCACT+AGG 0.548072 1:-76345768 None:intergenic
GCAATTGACACAACTGGTGC+TGG 0.548770 1:+76346785 MsG0180004312.01.T01:CDS
AATGAATATAGCTAGTCCTC+TGG 0.549042 1:-76347163 None:intergenic
TATGCTTTCATCAAAGCCCC+TGG 0.549304 1:+76344901 MsG0180004312.01.T01:CDS
ATTATCACTGATGGAGAGAA+GGG 0.554637 1:+76346257 MsG0180004312.01.T01:CDS
GAATGAGGCTGATTTCATCA+AGG 0.558047 1:+76346001 MsG0180004312.01.T01:CDS
GGATTATCATCAAATTTCAA+AGG 0.559045 1:-76347142 None:intergenic
TAGGGGATGATGAGTTTGGA+AGG 0.562525 1:+76345613 MsG0180004312.01.T01:CDS
CAGCACAAGCCACATTGGCA+GGG 0.565146 1:-76344930 None:intergenic
AGCACAAGCCACATTGGCAG+GGG 0.566309 1:-76344929 None:intergenic
GCCTCAGCAGAAGGCCACAA+AGG 0.575264 1:-76345951 None:intergenic
GCCATATGAGCAGATCTACA+TGG 0.575838 1:-76345867 None:intergenic
TCAGTAACATATCAGCACTA+GGG 0.589086 1:-76345767 None:intergenic
AGCATACCTCAAATATAGAA+GGG 0.591278 1:-76346850 None:intergenic
GGACACAGCACAAGCCACAT+TGG 0.596726 1:-76344935 None:intergenic
GCCATGAAAGCTGCTAGAGA+AGG 0.601701 1:+76345891 MsG0180004312.01.T01:CDS
CAATGCTGCCTTCATCGGCA+AGG 0.604108 1:+76344969 MsG0180004312.01.T01:CDS
TGGCGGTGCCCCTGCCAATG+TGG 0.604697 1:+76344921 MsG0180004312.01.T01:CDS
TCGCCGAATGATATCACCAA+TGG 0.606095 1:-76344826 None:intergenic
AGCAATGTGTACAACACAGA+AGG 0.614417 1:+76347046 MsG0180004312.01.T01:CDS
GGGTTGTAGATATGTCACTA+AGG 0.615724 1:+76346277 MsG0180004312.01.T01:CDS
GGTAAGTGGGTTCTCAGTGA+AGG 0.620599 1:+76346763 MsG0180004312.01.T01:CDS
GAAATTTGATGATAATCCAG+AGG 0.620620 1:+76347147 MsG0180004312.01.T01:three_prime_UTR
AAAACAATAATAACTGACAA+AGG 0.628440 1:+76344764 MsG0180004312.01.T01:five_prime_UTR
GAAAGAATCTGAATTGAACA+TGG 0.634038 1:+76345788 MsG0180004312.01.T01:CDS
GCAATGTGTACAACACAGAA+GGG 0.647308 1:+76347047 MsG0180004312.01.T01:CDS
ATGAAAGCTGCTAGAGAAGG+TGG 0.665141 1:+76345894 MsG0180004312.01.T01:CDS
GAAAGCATAAGATTCAGCCA+AGG 0.678603 1:-76344887 None:intergenic
GCTTTCATCAAAGCCCCTGG+CGG 0.681461 1:+76344904 MsG0180004312.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATAATCTTCTTGAA+TGG - Chr1:76346436-76346455 None:intergenic 15.0%
!! ATATATACTTAAAATGAATG+CGG + Chr1:76345050-76345069 MsG0180004312.01.T01:intron 15.0%
!! TAAAAATAATCTTCTTGAAT+GGG - Chr1:76346435-76346454 None:intergenic 15.0%
!! TTTGTAAAAATAATTCTCTT+TGG + Chr1:76346938-76346957 MsG0180004312.01.T01:intron 15.0%
!!! AAAATACTAATTTTAAGTCT+AGG + Chr1:76346914-76346933 MsG0180004312.01.T01:intron 15.0%
!!! ATAGAATATTGATAATATGA+GGG + Chr1:76345508-76345527 MsG0180004312.01.T01:intron 15.0%
!!! ATTTTTATCTATTCACAAAT+AGG + Chr1:76346448-76346467 MsG0180004312.01.T01:intron 15.0%
!!! TATAGAATATTGATAATATG+AGG + Chr1:76345507-76345526 MsG0180004312.01.T01:intron 15.0%
!!! TTGAATTTGAAATTTCTTTA+TGG + Chr1:76346036-76346055 MsG0180004312.01.T01:intron 15.0%
!!! TTTTTATCTATTCACAAATA+GGG + Chr1:76346449-76346468 MsG0180004312.01.T01:intron 15.0%
!! AAAACAATAATAACTGACAA+AGG + Chr1:76344764-76344783 MsG0180004312.01.T01:five_prime_UTR 20.0%
!! AATACAAAGATATTCCATTA+TGG + Chr1:76345822-76345841 MsG0180004312.01.T01:CDS 20.0%
!! ATAAATTTCAATGACCATAT+TGG - Chr1:76345248-76345267 None:intergenic 20.0%
!! ATATGGTCATTGAAATTTAT+AGG + Chr1:76345248-76345267 MsG0180004312.01.T01:intron 20.0%
!! GATAAACATGATAGTAAAAT+GGG - Chr1:76345014-76345033 None:intergenic 20.0%
!! TTTAAAAGAATCTGTAAAAG+TGG + Chr1:76346109-76346128 MsG0180004312.01.T01:intron 20.0%
!!! GCATTCATTTTAAGTATATA+TGG - Chr1:76345051-76345070 None:intergenic 20.0%
!!! TTGTATGAAAGTCTTATTAA+AGG - Chr1:76347206-76347225 None:intergenic 20.0%
! AAACTTACCATTCTCTATAT+AGG + Chr1:76346142-76346161 MsG0180004312.01.T01:intron 25.0%
! AAGATAAAATTAAGTCAAGC+TGG - Chr1:76346627-76346646 None:intergenic 25.0%
! AGATAAAATTAAGTCAAGCT+GGG - Chr1:76346626-76346645 None:intergenic 25.0%
! ATTTCAAAGGAAAGTTTAGT+TGG - Chr1:76347132-76347151 None:intergenic 25.0%
! CGATAAACATGATAGTAAAA+TGG - Chr1:76345015-76345034 None:intergenic 25.0%
! CTTTATTGAATTCAACATGA+AGG + Chr1:76346977-76346996 MsG0180004312.01.T01:intron 25.0%
! GGATTATCATCAAATTTCAA+AGG - Chr1:76347145-76347164 None:intergenic 25.0%
! TATGGAAGTCTATTAAATGA+TGG + Chr1:76346054-76346073 MsG0180004312.01.T01:intron 25.0%
! TCAAACAATATTTCCAAAGA+TGG - Chr1:76346723-76346742 None:intergenic 25.0%
! TGTCTCACAAATATAGTTTA+AGG - Chr1:76344722-76344741 None:intergenic 25.0%
! TTCTGAGTAAATATCCAATA+TGG + Chr1:76345231-76345250 MsG0180004312.01.T01:intron 25.0%
!! AAAGAGCTCTTTTCAAAATA+TGG - Chr1:76346318-76346337 None:intergenic 25.0%
!! GTTTGAATGCAGAATTTTAA+AGG + Chr1:76346737-76346756 MsG0180004312.01.T01:intron 25.0%
!! TTTTGTTAACTGATCATTAG+TGG + Chr1:76346369-76346388 MsG0180004312.01.T01:intron 25.0%
!!! AATGCAGAATTTTAAAGGAA+GGG + Chr1:76346742-76346761 MsG0180004312.01.T01:intron 25.0%
!!! AATGCTTTATTGCATTGATT+TGG - Chr1:76346351-76346370 None:intergenic 25.0%
!!! CGTATGTCATTTTTTTTGTT+AGG - Chr1:76345588-76345607 None:intergenic 25.0%
!!! TTTTCAAAATATGGATTGGA+TGG - Chr1:76346309-76346328 None:intergenic 25.0%
AAAAATGGAGTGAATACAGA+TGG + Chr1:76345657-76345676 MsG0180004312.01.T01:CDS 30.0%
AAGCATACCTCAAATATAGA+AGG - Chr1:76346854-76346873 None:intergenic 30.0%
AGCATACCTCAAATATAGAA+GGG - Chr1:76346853-76346872 None:intergenic 30.0%
ATACTTAAAATGAATGCGGA+TGG + Chr1:76345054-76345073 MsG0180004312.01.T01:intron 30.0%
ATTTACGTATAGTCTACCAA+CGG - Chr1:76345148-76345167 None:intergenic 30.0%
CAAAAAAAATGACATACGAC+AGG + Chr1:76345590-76345609 MsG0180004312.01.T01:intron 30.0%
CTAAACTATTCATCCATCTT+TGG + Chr1:76346707-76346726 MsG0180004312.01.T01:intron 30.0%
GAAAATAGGATACCAACTTA+TGG - Chr1:76346566-76346585 None:intergenic 30.0%
GAAAGAATCTGAATTGAACA+TGG + Chr1:76345788-76345807 MsG0180004312.01.T01:CDS 30.0%
GAGGATATAACAGAAAACTA+GGG + Chr1:76346076-76346095 MsG0180004312.01.T01:intron 30.0%
GAGTAGATATCCTGTTTAAT+CGG - Chr1:76345557-76345576 None:intergenic 30.0%
GTTTGATGTGGTATTATATG+AGG + Chr1:76345184-76345203 MsG0180004312.01.T01:intron 30.0%
TACTTAAAATGAATGCGGAT+GGG + Chr1:76345055-76345074 MsG0180004312.01.T01:intron 30.0%
TATGGAGAGCTTATGAAAAT+AGG - Chr1:76346580-76346599 None:intergenic 30.0%
TCACTTACCTATATAGAGAA+TGG - Chr1:76346152-76346171 None:intergenic 30.0%
TTATCTTAGCAAATGCATCT+CGG + Chr1:76345391-76345410 MsG0180004312.01.T01:intron 30.0%
! ACTGTTTTCATAAGCTACTA+TGG - Chr1:76346598-76346617 None:intergenic 30.0%
! GAAATTTGATGATAATCCAG+AGG + Chr1:76347147-76347166 MsG0180004312.01.T01:three_prime_UTR 30.0%
! GCTCTTTTCAAAATATGGAT+TGG - Chr1:76346313-76346332 None:intergenic 30.0%
! TAAGACTAATGGATCCATAA+TGG - Chr1:76345839-76345858 None:intergenic 30.0%
!! AATCAAAAGCATTTGGAATG+AGG + Chr1:76345986-76346005 MsG0180004312.01.T01:CDS 30.0%
!! AATTTTAAAGGAAGGGTAAG+TGG + Chr1:76346749-76346768 MsG0180004312.01.T01:CDS 30.0%
!! ATTTTAAAGGAAGGGTAAGT+GGG + Chr1:76346750-76346769 MsG0180004312.01.T01:CDS 30.0%
!! GAATGCAGAATTTTAAAGGA+AGG + Chr1:76346741-76346760 MsG0180004312.01.T01:intron 30.0%
!! TGGTTCTGAAATAAGACTAA+TGG - Chr1:76345850-76345869 None:intergenic 30.0%
AAAAATGACATACGACAGGT+AGG + Chr1:76345594-76345613 MsG0180004312.01.T01:intron 35.0%
AAAATGACATACGACAGGTA+GGG + Chr1:76345595-76345614 MsG0180004312.01.T01:intron 35.0%
AAGAAGAAGAACAAGATGTC+AGG + Chr1:76344793-76344812 MsG0180004312.01.T01:exon 35.0%
AAGAAGCTGTTATGTCTCTA+TGG + Chr1:76346213-76346232 MsG0180004312.01.T01:CDS 35.0%
AAGAGACCCTTCTATATTTG+AGG + Chr1:76346844-76346863 MsG0180004312.01.T01:CDS 35.0%
AAGCTGCTTATTATCACTGA+TGG + Chr1:76346248-76346267 MsG0180004312.01.T01:CDS 35.0%
AATGAATATAGCTAGTCCTC+TGG - Chr1:76347166-76347185 None:intergenic 35.0%
ATAACAGCTTCTTCATTCTC+AGG - Chr1:76346206-76346225 None:intergenic 35.0%
ATAGCCTCCGTAAATATGAA+TGG + Chr1:76345443-76345462 MsG0180004312.01.T01:intron 35.0%
ATTATCACTGATGGAGAGAA+GGG + Chr1:76346257-76346276 MsG0180004312.01.T01:CDS 35.0%
CGGAGGCTATTAAATTGAAA+TGG - Chr1:76345433-76345452 None:intergenic 35.0%
CGTCCTTCCATTCATATTTA+CGG - Chr1:76345453-76345472 None:intergenic 35.0%
CTATACGTAAATATTCACCG+AGG + Chr1:76345156-76345175 MsG0180004312.01.T01:intron 35.0%
CTTACTAGATGTCCATAAGT+TGG + Chr1:76346551-76346570 MsG0180004312.01.T01:intron 35.0%
GACTAGATTGATATGGTCAA+GGG + Chr1:76345530-76345549 MsG0180004312.01.T01:intron 35.0%
GCAGACATCTTGAAGAAAAA+TGG + Chr1:76345642-76345661 MsG0180004312.01.T01:CDS 35.0%
GGAAGTCTATTAAATGATGG+AGG + Chr1:76346057-76346076 MsG0180004312.01.T01:intron 35.0%
GGAGGATATAACAGAAAACT+AGG + Chr1:76346075-76346094 MsG0180004312.01.T01:intron 35.0%
GTAAATATTCACCGAGGTTA+TGG + Chr1:76345162-76345181 MsG0180004312.01.T01:intron 35.0%
TATGAGGGACTAGATTGATA+TGG + Chr1:76345523-76345542 MsG0180004312.01.T01:intron 35.0%
TATTATCACTGATGGAGAGA+AGG + Chr1:76346256-76346275 MsG0180004312.01.T01:CDS 35.0%
TCAGTAACATATCAGCACTA+GGG - Chr1:76345770-76345789 None:intergenic 35.0%
TGTTCGAAACAGTCTTACAA+GGG - Chr1:76346517-76346536 None:intergenic 35.0%
TTCAGTAACATATCAGCACT+AGG - Chr1:76345771-76345790 None:intergenic 35.0%
TTGACATTTGCAAATGCTTG+TGG + Chr1:76347023-76347042 MsG0180004312.01.T01:CDS 35.0%
TTGCTAAGATAACACAACTG+AGG - Chr1:76345383-76345402 None:intergenic 35.0%
TTGTTCGAAACAGTCTTACA+AGG - Chr1:76346518-76346537 None:intergenic 35.0%
TTTACGTATAGTCTACCAAC+GGG - Chr1:76345147-76345166 None:intergenic 35.0%
TTTGTGACATTGAGGAAAGA+TGG + Chr1:76345717-76345736 MsG0180004312.01.T01:CDS 35.0%
! GATCCATTGGTGATATCATT+CGG + Chr1:76344823-76344842 MsG0180004312.01.T01:CDS 35.0%
! GTATTTTTCTGTCACTAGAG+TGG - Chr1:76346901-76346920 None:intergenic 35.0%
! GTCAAAGTCTCTCTCAATTT+TGG - Chr1:76347008-76347027 None:intergenic 35.0%
AAATGACATACGACAGGTAG+GGG + Chr1:76345596-76345615 MsG0180004312.01.T01:intron 40.0%
AAGCCTGACATTTGGATCAT+AGG - Chr1:76345931-76345950 None:intergenic 40.0%
ACCACATCAAACCATAACCT+CGG - Chr1:76345176-76345195 None:intergenic 40.0%
ACCATGTAGATCTGCTCATA+TGG + Chr1:76345866-76345885 MsG0180004312.01.T01:CDS 40.0%
AGCAATGTGTACAACACAGA+AGG + Chr1:76347046-76347065 MsG0180004312.01.T01:CDS 40.0%
AGTCTTACAAGGGCTTATGT+CGG - Chr1:76346507-76346526 None:intergenic 40.0%
ATTAAGAACCTTGCCGATGA+AGG - Chr1:76344980-76344999 None:intergenic 40.0%
ATTGAGGAAAGATGGAGAGA+GGG + Chr1:76345725-76345744 MsG0180004312.01.T01:CDS 40.0%
CCTCCGTAAATATGAATGGA+AGG + Chr1:76345447-76345466 MsG0180004312.01.T01:intron 40.0%
CCTTCCATTCATATTTACGG+AGG - Chr1:76345450-76345469 None:intergenic 40.0%
GCAATGTGTACAACACAGAA+GGG + Chr1:76347047-76347066 MsG0180004312.01.T01:CDS 40.0%
GGACTAGATTGATATGGTCA+AGG + Chr1:76345529-76345548 MsG0180004312.01.T01:intron 40.0%
GGGTTGTAGATATGTCACTA+AGG + Chr1:76346277-76346296 MsG0180004312.01.T01:CDS 40.0%
TAAATCGCAGCTAATGCAAG+CGG - Chr1:76345105-76345124 None:intergenic 40.0%
TGTCCTATGATCCAAATGTC+AGG + Chr1:76345925-76345944 MsG0180004312.01.T01:CDS 40.0%
TTACGTATAGTCTACCAACG+GGG - Chr1:76345146-76345165 None:intergenic 40.0%
! GAAAGCATAAGATTCAGCCA+AGG - Chr1:76344890-76344909 None:intergenic 40.0%
! GAATGAGGCTGATTTCATCA+AGG + Chr1:76346001-76346020 MsG0180004312.01.T01:CDS 40.0%
! GATTTTGTTCCAGACACATC+TGG + Chr1:76344859-76344878 MsG0180004312.01.T01:CDS 40.0%
! TGGTGTTCTTTTTGACACAG+AGG + Chr1:76345677-76345696 MsG0180004312.01.T01:CDS 40.0%
!! AGTTTAGTTGGCTTTAGAGC+TGG - Chr1:76347120-76347139 None:intergenic 40.0%
!! GGTCTGGAATCAAAAGCATT+TGG + Chr1:76345979-76345998 MsG0180004312.01.T01:CDS 40.0%
!!! CATTGGCTTTTGTGACATTG+AGG + Chr1:76345709-76345728 MsG0180004312.01.T01:CDS 40.0%
AAGGATACACCAGATGTGTC+TGG - Chr1:76344871-76344890 None:intergenic 45.0%
ACCGAGGTTATGGTTTGATG+TGG + Chr1:76345172-76345191 MsG0180004312.01.T01:intron 45.0%
ACCTTCTCTAGCAGCTTTCA+TGG - Chr1:76345895-76345914 None:intergenic 45.0%
AGACACATCTGGTGTATCCT+TGG + Chr1:76344870-76344889 MsG0180004312.01.T01:CDS 45.0%
ATGAAAGCTGCTAGAGAAGG+TGG + Chr1:76345894-76345913 MsG0180004312.01.T01:CDS 45.0%
CAAATGTCAGGCTTCCTTTG+TGG + Chr1:76345937-76345956 MsG0180004312.01.T01:CDS 45.0%
CACAAAGGAAGCCTGACATT+TGG - Chr1:76345939-76345958 None:intergenic 45.0%
CATTGAGGAAAGATGGAGAG+AGG + Chr1:76345724-76345743 MsG0180004312.01.T01:CDS 45.0%
GCCATATGAGCAGATCTACA+TGG - Chr1:76345870-76345889 None:intergenic 45.0%
GCTTGCATTAGCTGCGATTT+AGG + Chr1:76345104-76345123 MsG0180004312.01.T01:intron 45.0%
GTCAGGCTCAAAAGATCCAT+TGG + Chr1:76344810-76344829 MsG0180004312.01.T01:CDS 45.0%
GTGAAGGCAATTGACACAAC+TGG + Chr1:76346779-76346798 MsG0180004312.01.T01:CDS 45.0%
TAGGGGATGATGAGTTTGGA+AGG + Chr1:76345613-76345632 MsG0180004312.01.T01:CDS 45.0%
TATGCTTTCATCAAAGCCCC+TGG + Chr1:76344901-76344920 MsG0180004312.01.T01:CDS 45.0%
TCGCCGAATGATATCACCAA+TGG - Chr1:76344829-76344848 None:intergenic 45.0%
!!! GGTGCTGGTGATTCTTTTGT+TGG + Chr1:76346800-76346819 MsG0180004312.01.T01:CDS 45.0%
CACAGAGGCAAGAACTGCAT+TGG + Chr1:76345692-76345711 MsG0180004312.01.T01:CDS 50.0%
CAGGTAGGGGATGATGAGTT+TGG + Chr1:76345609-76345628 MsG0180004312.01.T01:intron 50.0%
CTAATCGAGAGTTTCCCCGT+TGG + Chr1:76345129-76345148 MsG0180004312.01.T01:intron 50.0%
GCAATTGACACAACTGGTGC+TGG + Chr1:76346785-76346804 MsG0180004312.01.T01:CDS 50.0%
GCCATGAAAGCTGCTAGAGA+AGG + Chr1:76345891-76345910 MsG0180004312.01.T01:CDS 50.0%
GCTTGTGCTGTGTCCAAACT+TGG + Chr1:76344943-76344962 MsG0180004312.01.T01:CDS 50.0%
GGTCAAGGGACCGATTAAAC+AGG + Chr1:76345544-76345563 MsG0180004312.01.T01:intron 50.0%
TGCACTTCCAACTGCTGAAG+AGG + Chr1:76347079-76347098 MsG0180004312.01.T01:CDS 50.0%
TGTGCTGTGTCCAAACTTGG+TGG + Chr1:76344946-76344965 MsG0180004312.01.T01:CDS 50.0%
TTCTGAGCCTCTTCAGCAGT+TGG - Chr1:76347089-76347108 None:intergenic 50.0%
! AGCAGTTGGAAGTGCAGGAA+TGG - Chr1:76347075-76347094 None:intergenic 50.0%
! GGTAAGTGGGTTCTCAGTGA+AGG + Chr1:76346763-76346782 MsG0180004312.01.T01:CDS 50.0%
! TCTTCAGCAGTTGGAAGTGC+AGG - Chr1:76347080-76347099 None:intergenic 50.0%
ACAGCACAAGCCACATTGGC+AGG - Chr1:76344934-76344953 None:intergenic 55.0%
AGCACAAGCCACATTGGCAG+GGG - Chr1:76344932-76344951 None:intergenic 55.0%
CAATGCTGCCTTCATCGGCA+AGG + Chr1:76344969-76344988 MsG0180004312.01.T01:CDS 55.0%
CAGCACAAGCCACATTGGCA+GGG - Chr1:76344933-76344952 None:intergenic 55.0%
GCTTTCATCAAAGCCCCTGG+CGG + Chr1:76344904-76344923 MsG0180004312.01.T01:CDS 55.0%
GGACACAGCACAAGCCACAT+TGG - Chr1:76344938-76344957 None:intergenic 55.0%
GGCAGCATTGCCACCAAGTT+TGG - Chr1:76344959-76344978 None:intergenic 55.0%
GGTGGCAATGCTGCCTTCAT+CGG + Chr1:76344964-76344983 MsG0180004312.01.T01:CDS 55.0%
TCCTTTGTGGCCTTCTGCTG+AGG + Chr1:76345950-76345969 MsG0180004312.01.T01:CDS 55.0%
TCGCAGCTAATGCAAGCGGA+AGG - Chr1:76345101-76345120 None:intergenic 55.0%
GACCTAGCAGCCTCAGCAGA+AGG - Chr1:76345963-76345982 None:intergenic 60.0%
GCCTCAGCAGAAGGCCACAA+AGG - Chr1:76345954-76345973 None:intergenic 60.0%
TCTGCTGAGGCTGCTAGGTC+TGG + Chr1:76345963-76345982 MsG0180004312.01.T01:CDS 60.0%
GGCCTTCTGCTGAGGCTGCT+AGG + Chr1:76345958-76345977 MsG0180004312.01.T01:CDS 65.0%
TGGCGGTGCCCCTGCCAATG+TGG + Chr1:76344921-76344940 MsG0180004312.01.T01:CDS 70.0%
!! ACATTGGCAGGGGCACCGCC+AGG - Chr1:76344922-76344941 None:intergenic 70.0%
!! ATTGGCAGGGGCACCGCCAG+GGG - Chr1:76344920-76344939 None:intergenic 70.0%
!! CATTGGCAGGGGCACCGCCA+GGG - Chr1:76344921-76344940 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 76344717 76347268 76344717 ID=MsG0180004312.01;Name=MsG0180004312.01
Chr1 mRNA 76344717 76347268 76344717 ID=MsG0180004312.01.T01;Parent=MsG0180004312.01;Name=MsG0180004312.01.T01;_AED=0.46;_eAED=0.46;_QI=91|1|1|1|1|1|5|132|327
Chr1 exon 76344717 76344990 76344717 ID=MsG0180004312.01.T01:exon:14802;Parent=MsG0180004312.01.T01
Chr1 exon 76345612 76346022 76345612 ID=MsG0180004312.01.T01:exon:14803;Parent=MsG0180004312.01.T01
Chr1 exon 76346164 76346298 76346164 ID=MsG0180004312.01.T01:exon:14804;Parent=MsG0180004312.01.T01
Chr1 exon 76346749 76346865 76346749 ID=MsG0180004312.01.T01:exon:14805;Parent=MsG0180004312.01.T01
Chr1 exon 76346999 76347268 76346999 ID=MsG0180004312.01.T01:exon:14806;Parent=MsG0180004312.01.T01
Chr1 five_prime_UTR 76344717 76344807 76344717 ID=MsG0180004312.01.T01:five_prime_utr;Parent=MsG0180004312.01.T01
Chr1 CDS 76344808 76344990 76344808 ID=MsG0180004312.01.T01:cds;Parent=MsG0180004312.01.T01
Chr1 CDS 76345612 76346022 76345612 ID=MsG0180004312.01.T01:cds;Parent=MsG0180004312.01.T01
Chr1 CDS 76346164 76346298 76346164 ID=MsG0180004312.01.T01:cds;Parent=MsG0180004312.01.T01
Chr1 CDS 76346749 76346865 76346749 ID=MsG0180004312.01.T01:cds;Parent=MsG0180004312.01.T01
Chr1 CDS 76346999 76347136 76346999 ID=MsG0180004312.01.T01:cds;Parent=MsG0180004312.01.T01
Chr1 three_prime_UTR 76347137 76347268 76347137 ID=MsG0180004312.01.T01:three_prime_utr;Parent=MsG0180004312.01.T01
Gene Sequence

>MsG0180004312.01.T01

ATGTCAGGCTCAAAAGATCCATTGGTGATATCATTCGGCGAGATGCTCATAGATTTTGTTCCAGACACATCTGGTGTATCCTTGGCTGAATCTTATGCTTTCATCAAAGCCCCTGGCGGTGCCCCTGCCAATGTGGCTTGTGCTGTGTCCAAACTTGGTGGCAATGCTGCCTTCATCGGCAAGGTAGGGGATGATGAGTTTGGAAGGATGCTAGCAGACATCTTGAAGAAAAATGGAGTGAATACAGATGGTGTTCTTTTTGACACAGAGGCAAGAACTGCATTGGCTTTTGTGACATTGAGGAAAGATGGAGAGAGGGAGTTCATGTTTTATAGAAACCCTAGTGCTGATATGTTACTGAAAGAATCTGAATTGAACATGGATATGATTAAGAATACAAAGATATTCCATTATGGATCCATTAGTCTTATTTCAGAACCATGTAGATCTGCTCATATGGCTGCCATGAAAGCTGCTAGAGAAGGTGGTGCTTTGTTGTCCTATGATCCAAATGTCAGGCTTCCTTTGTGGCCTTCTGCTGAGGCTGCTAGGTCTGGAATCAAAAGCATTTGGAATGAGGCTGATTTCATCAAGGTAAGTGATGATGAAGTTCAATTTCTTACACAAAAAGATCCTGAGAATGAAGAAGCTGTTATGTCTCTATGGCATGACAAGTTGAAGCTGCTTATTATCACTGATGGAGAGAAGGGTTGTAGATATGTCACTAAGAATTTTAAAGGAAGGGTAAGTGGGTTCTCAGTGAAGGCAATTGACACAACTGGTGCTGGTGATTCTTTTGTTGGTGCACTTCTCAGAGATGTAGCAAGAGACCCTTCTATATTTGAGGATGAACCAAAATTGAGAGAGACTTTGACATTTGCAAATGCTTGTGGAGCAATGTGTACAACACAGAAGGGAGCCATTCCTGCACTTCCAACTGCTGAAGAGGCTCAGAAATTTATTTCCAGCTCTAAAGCCAACTAA
Protein sequence

>MsG0180004312.01.T01

MSGSKDPLVISFGEMLIDFVPDTSGVSLAESYAFIKAPGGAPANVACAVSKLGGNAAFIGKVGDDEFGRMLADILKKNGVNTDGVLFDTEARTALAFVTLRKDGEREFMFYRNPSADMLLKESELNMDMIKNTKIFHYGSISLISEPCRSAHMAAMKAAREGGALLSYDPNVRLPLWPSAEAARSGIKSIWNEADFIKVSDDEVQFLTQKDPENEEAVMSLWHDKLKLLIITDGEKGCRYVTKNFKGRVSGFSVKAIDTTGAGDSFVGALLRDVARDPSIFEDEPKLRETLTFANACGAMCTTQKGAIPALPTAEEAQKFISSSKAN*