AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038417.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038417.01.T02 MTR_7g055743 89.071 183 20 0 1 183 187 369 9.70e-117 336
MsG0780038417.01.T02 MTR_4g036260 69.101 178 55 0 1 178 126 303 1.74e-87 259
MsG0780038417.01.T02 MTR_4g036310 67.416 178 58 0 1 178 147 324 4.93e-85 254
MsG0780038417.01.T02 MTR_4g033205 66.292 178 60 0 1 178 147 324 1.72e-81 245
MsG0780038417.01.T02 MTR_4g032995 66.292 178 60 0 1 178 147 324 1.72e-81 245
MsG0780038417.01.T02 MTR_4g036170 64.045 178 64 0 1 178 122 299 7.56e-77 232
MsG0780038417.01.T02 MTR_8g064610 31.977 172 93 5 1 168 216 367 6.75e-13 66.2
MsG0780038417.01.T02 MTR_4g086400 33.133 166 88 5 11 157 433 594 3.36e-11 61.6
MsG0780038417.01.T02 MTR_4g086400 33.133 166 88 5 11 157 293 454 3.45e-11 61.6
MsG0780038417.01.T01 MTR_7g055743 88.830 188 21 0 3 190 182 369 4.74e-119 342
MsG0780038417.01.T01 MTR_4g036260 69.101 178 55 0 8 185 126 303 1.16e-87 260
MsG0780038417.01.T01 MTR_4g036310 65.761 184 63 0 2 185 141 324 1.46e-85 256
MsG0780038417.01.T01 MTR_4g033205 65.027 183 64 0 3 185 142 324 9.13e-82 246
MsG0780038417.01.T01 MTR_4g032995 65.027 183 64 0 3 185 142 324 1.02e-81 246
MsG0780038417.01.T01 MTR_4g036170 64.045 178 64 0 8 185 122 299 5.31e-77 233
MsG0780038417.01.T01 MTR_8g064610 31.977 172 93 5 8 175 216 367 7.85e-13 66.2
MsG0780038417.01.T01 MTR_4g086400 32.967 182 98 6 3 164 277 454 1.29e-11 63.2
MsG0780038417.01.T01 MTR_4g086400 32.967 182 98 6 3 164 417 594 1.56e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038417.01.T02 AT4G13010 73.034 178 48 0 1 178 147 324 1.52e-93 276
MsG0780038417.01.T02 AT1G23740 33.939 165 85 6 8 168 225 369 2.35e-11 62.0
MsG0780038417.01.T01 AT4G13010 70.811 185 54 0 1 185 140 324 6.86e-94 277
MsG0780038417.01.T01 AT1G23740 33.939 165 85 6 15 175 225 369 2.32e-11 62.4

Find 37 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCCTTAGCTAGAGGAAATT+TGG 0.203726 7:-46398480 None:intergenic
CTCTTTAATTCCACACCAAT+TGG 0.361493 7:-46398200 None:intergenic
TTACCATTCGTGTTCAAGTT+AGG 0.361630 7:-46398290 None:intergenic
GAGTATTGATGTCATGCAAC+TGG 0.368130 7:+46398520 MsG0780038417.01.T01:CDS
AACTGAAGATCCTTACCCTT+TGG 0.395500 7:-46398410 None:intergenic
TCCATCGAGTTTGATTCCTA+TGG 0.412049 7:-46398009 None:intergenic
GTGGAATTAAAGAGTCCTTC+TGG 0.426954 7:+46398209 MsG0780038417.01.T01:CDS
ATGGAAATTGTCAAAAGCTT+AGG 0.438856 7:+46398149 MsG0780038417.01.T01:CDS
TTCCATGTTTCGAGCGCCGC+AGG 0.443235 7:-46398132 None:intergenic
GCTTCTAATTCCAAAGGGTA+AGG 0.460785 7:+46398400 MsG0780038417.01.T01:CDS
ATTGACTATAAGACACCAAT+TGG 0.468885 7:+46398185 MsG0780038417.01.T01:CDS
CCCTTTGGAATTAGAAGCAA+AGG 0.479238 7:-46398395 None:intergenic
CTTGTTGATTTGATTAAGCA+AGG 0.495471 7:+46398434 MsG0780038417.01.T01:CDS
GCCTTTGCTTCTAATTCCAA+AGG 0.500098 7:+46398394 MsG0780038417.01.T01:CDS
CCTCTAGCTAAGGCTGAAGA+TGG 0.513384 7:+46398488 MsG0780038417.01.T01:CDS
CACCTGCGGCGCTCGAAACA+TGG 0.515827 7:+46398130 MsG0780038417.01.T01:CDS
CTCCAAATTTCCTCTAGCTA+AGG 0.534188 7:+46398478 MsG0780038417.01.T01:CDS
CCTTTGCTTCTAATTCCAAA+GGG 0.536074 7:+46398395 MsG0780038417.01.T01:CDS
GAGCCTAACTTGAACACGAA+TGG 0.537643 7:+46398287 MsG0780038417.01.T01:CDS
ACGGCTGCATCAGGCGGTGT+AGG 0.539156 7:+46398059 MsG0780038417.01.T01:CDS
CATGTTTCGAGCGCCGCAGG+TGG 0.554237 7:-46398129 None:intergenic
TAGCTAAGGCTGAAGATGGT+TGG 0.558067 7:+46398492 MsG0780038417.01.T01:CDS
TCCATAGGAATCAAACTCGA+TGG 0.568998 7:+46398008 MsG0780038417.01.T01:CDS
CAAAAGCTTAGGAGCTGATG+AGG 0.570564 7:+46398160 MsG0780038417.01.T01:CDS
GCATCATATTTCTTACCAGA+AGG 0.581763 7:-46398224 None:intergenic
GTCAACATTGAAGTAGAGCT+AGG 0.594837 7:-46398329 None:intergenic
TAACTTGAACACGAATGGTA+AGG 0.595964 7:+46398292 MsG0780038417.01.T01:CDS
AGTATTGATGTCATGCAACT+GGG 0.597965 7:+46398521 MsG0780038417.01.T01:CDS
GCAGTTCAATTAGCAAAGCT+AGG 0.598271 7:+46398089 MsG0780038417.01.T01:CDS
TTGATCACGGCTGCATCAGG+CGG 0.599890 7:+46398053 MsG0780038417.01.T01:CDS
ATTGTGCAACGGATATTGCA+TGG 0.613154 7:+46398255 MsG0780038417.01.T01:CDS
CCATCTTCAGCCTTAGCTAG+AGG 0.614800 7:-46398488 None:intergenic
TGGTAAGAAATATGATGCAG+TGG 0.629719 7:+46398229 MsG0780038417.01.T01:CDS
CTATAAGACACCAATTGGTG+TGG 0.632516 7:+46398190 MsG0780038417.01.T01:CDS
TGCAGTGGTTCATTGTGCAA+CGG 0.675224 7:+46398244 MsG0780038417.01.T01:CDS
AACTCGATGGAACTAGCGAG+CGG 0.716496 7:+46398021 MsG0780038417.01.T01:CDS
ACTCACGTAACAGCCACCTG+CGG 0.724816 7:+46398116 MsG0780038417.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTTTGATCAAATCCAT+AGG + Chr7:46397993-46398012 MsG0780038417.01.T01:CDS 25.0%
ATGGAAATTGTCAAAAGCTT+AGG + Chr7:46398149-46398168 MsG0780038417.01.T01:CDS 30.0%
ATTGACTATAAGACACCAAT+TGG + Chr7:46398185-46398204 MsG0780038417.01.T01:CDS 30.0%
!! CTTGTTGATTTGATTAAGCA+AGG + Chr7:46398434-46398453 MsG0780038417.01.T01:CDS 30.0%
CCTTTGCTTCTAATTCCAAA+GGG + Chr7:46398395-46398414 MsG0780038417.01.T01:CDS 35.0%
CTCTTTAATTCCACACCAAT+TGG - Chr7:46398203-46398222 None:intergenic 35.0%
GCATCATATTTCTTACCAGA+AGG - Chr7:46398227-46398246 None:intergenic 35.0%
TAACTTGAACACGAATGGTA+AGG + Chr7:46398292-46398311 MsG0780038417.01.T01:CDS 35.0%
TGGTAAGAAATATGATGCAG+TGG + Chr7:46398229-46398248 MsG0780038417.01.T01:CDS 35.0%
! TTACCATTCGTGTTCAAGTT+AGG - Chr7:46398293-46398312 None:intergenic 35.0%
!! AGTATTGATGTCATGCAACT+GGG + Chr7:46398521-46398540 MsG0780038417.01.T01:CDS 35.0%
!! GCGGAAAAACATTTTGATCA+CGG + Chr7:46398040-46398059 MsG0780038417.01.T01:CDS 35.0%
!!! CAAAGGCACAAGTTGTTTTT+TGG - Chr7:46398381-46398400 None:intergenic 35.0%
!!! CACAAGTTGTTTTTTGGAGA+AGG - Chr7:46398375-46398394 None:intergenic 35.0%
AACTGAAGATCCTTACCCTT+TGG - Chr7:46398413-46398432 None:intergenic 40.0%
AGCCTTAGCTAGAGGAAATT+TGG - Chr7:46398483-46398502 None:intergenic 40.0%
ATTGTGCAACGGATATTGCA+TGG + Chr7:46398255-46398274 MsG0780038417.01.T01:CDS 40.0%
CTATAAGACACCAATTGGTG+TGG + Chr7:46398190-46398209 MsG0780038417.01.T01:CDS 40.0%
CTCCAAATTTCCTCTAGCTA+AGG + Chr7:46398478-46398497 MsG0780038417.01.T01:CDS 40.0%
GCAGTTCAATTAGCAAAGCT+AGG + Chr7:46398089-46398108 MsG0780038417.01.T01:CDS 40.0%
GCCTTTGCTTCTAATTCCAA+AGG + Chr7:46398394-46398413 MsG0780038417.01.T01:CDS 40.0%
GCTTCTAATTCCAAAGGGTA+AGG + Chr7:46398400-46398419 MsG0780038417.01.T01:CDS 40.0%
GTCAACATTGAAGTAGAGCT+AGG - Chr7:46398332-46398351 None:intergenic 40.0%
GTGGAATTAAAGAGTCCTTC+TGG + Chr7:46398209-46398228 MsG0780038417.01.T01:CDS 40.0%
TCCATAGGAATCAAACTCGA+TGG + Chr7:46398008-46398027 MsG0780038417.01.T01:CDS 40.0%
TCCATCGAGTTTGATTCCTA+TGG - Chr7:46398012-46398031 None:intergenic 40.0%
!! CCCTTTGGAATTAGAAGCAA+AGG - Chr7:46398398-46398417 None:intergenic 40.0%
!! GAGTATTGATGTCATGCAAC+TGG + Chr7:46398520-46398539 MsG0780038417.01.T01:CDS 40.0%
CAAAAGCTTAGGAGCTGATG+AGG + Chr7:46398160-46398179 MsG0780038417.01.T01:CDS 45.0%
GAGCCTAACTTGAACACGAA+TGG + Chr7:46398287-46398306 MsG0780038417.01.T01:CDS 45.0%
TAGCTAAGGCTGAAGATGGT+TGG + Chr7:46398492-46398511 MsG0780038417.01.T01:CDS 45.0%
TGCAGTGGTTCATTGTGCAA+CGG + Chr7:46398244-46398263 MsG0780038417.01.T01:CDS 45.0%
!! ATTTTGATCACGGCTGCATC+AGG + Chr7:46398050-46398069 MsG0780038417.01.T01:CDS 45.0%
AACTCGATGGAACTAGCGAG+CGG + Chr7:46398021-46398040 MsG0780038417.01.T01:CDS 50.0%
CCATCTTCAGCCTTAGCTAG+AGG - Chr7:46398491-46398510 None:intergenic 50.0%
CCTCTAGCTAAGGCTGAAGA+TGG + Chr7:46398488-46398507 MsG0780038417.01.T01:CDS 50.0%
ACTCACGTAACAGCCACCTG+CGG + Chr7:46398116-46398135 MsG0780038417.01.T01:CDS 55.0%
TTGATCACGGCTGCATCAGG+CGG + Chr7:46398053-46398072 MsG0780038417.01.T01:CDS 55.0%
! TTCCATGTTTCGAGCGCCGC+AGG - Chr7:46398135-46398154 None:intergenic 60.0%
ACGGCTGCATCAGGCGGTGT+AGG + Chr7:46398059-46398078 MsG0780038417.01.T01:CDS 65.0%
CACCTGCGGCGCTCGAAACA+TGG + Chr7:46398130-46398149 MsG0780038417.01.T01:CDS 65.0%
! CATGTTTCGAGCGCCGCAGG+TGG - Chr7:46398132-46398151 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 46397993 46398586 46397993 ID=MsG0780038417.01;Name=MsG0780038417.01
Chr7 mRNA 46397993 46398586 46397993 ID=MsG0780038417.01.T01;Parent=MsG0780038417.01;Name=MsG0780038417.01.T01;_AED=0.11;_eAED=0.14;_QI=0|-1|0|1|-1|1|1|0|197
Chr7 exon 46397993 46398586 46397993 ID=MsG0780038417.01.T01:exon:5249;Parent=MsG0780038417.01.T01
Chr7 CDS 46397993 46398586 46397993 ID=MsG0780038417.01.T01:cds;Parent=MsG0780038417.01.T01
Chr7 mRNA 46398014 46398586 46398014 ID=MsG0780038417.01.T02;Parent=MsG0780038417.01;Name=MsG0780038417.01.T02;_AED=0.12;_eAED=0.16;_QI=0|-1|0|1|-1|1|1|0|190
Chr7 exon 46398014 46398586 46398014 ID=MsG0780038417.01.T02:exon:5250;Parent=MsG0780038417.01.T02
Chr7 CDS 46398014 46398586 46398014 ID=MsG0780038417.01.T02:cds;Parent=MsG0780038417.01.T02
Chr7 mRNA 46398507 46398557 46398507 ID=MsG0780038417.01.T03;Parent=MsG0780038417.01;Name=MsG0780038417.01.T03;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|16
Chr7 exon 46398507 46398557 46398507 ID=MsG0780038417.01.T03:exon:5251;Parent=MsG0780038417.01.T03
Chr7 CDS 46398507 46398557 46398507 ID=MsG0780038417.01.T03:cds;Parent=MsG0780038417.01.T03
Gene Sequence

>MsG0780038417.01.T02

GGAATCAAACTCGATGGAACTAGCGAGCGGAAAAACATTTTGATCACGGCTGCATCAGGCGGTGTAGGACACTATGCAGTTCAATTAGCAAAGCTAGGTAACACTCACGTAACAGCCACCTGCGGCGCTCGAAACATGGAAATTGTCAAAAGCTTAGGAGCTGATGAGGTAATTGACTATAAGACACCAATTGGTGTGGAATTAAAGAGTCCTTCTGGTAAGAAATATGATGCAGTGGTTCATTGTGCAACGGATATTGCATGGTCAGTTTTTGAGCCTAACTTGAACACGAATGGTAAGGTTGTAGATATAACTCCTAGCTCTACTTCAATGTTGACATTTGCTCTTACTAAAATTACCTTCTCCAAAAAACAACTTGTGCCTTTGCTTCTAATTCCAAAGGGTAAGGATCTTCAGTTTCTTGTTGATTTGATTAAGCAAGGAAAGATTAAAACAGTTATAGACTCCAAATTTCCTCTAGCTAAGGCTGAAGATGGTTGGTCTAAGAGTATTGATGTCATGCAACTGGGAAGATCATTTTTGAGTTTTAATTATGCTTATAGTGCTTTGTAA

>MsG0780038417.01.T03

ATGGTTGGTCTAAGAGTATTGATGTCATGCAACTGGGAAGATCATTTTTGA

>MsG0780038417.01.T01

AAGATTTTGATCAAATCCATAGGAATCAAACTCGATGGAACTAGCGAGCGGAAAAACATTTTGATCACGGCTGCATCAGGCGGTGTAGGACACTATGCAGTTCAATTAGCAAAGCTAGGTAACACTCACGTAACAGCCACCTGCGGCGCTCGAAACATGGAAATTGTCAAAAGCTTAGGAGCTGATGAGGTAATTGACTATAAGACACCAATTGGTGTGGAATTAAAGAGTCCTTCTGGTAAGAAATATGATGCAGTGGTTCATTGTGCAACGGATATTGCATGGTCAGTTTTTGAGCCTAACTTGAACACGAATGGTAAGGTTGTAGATATAACTCCTAGCTCTACTTCAATGTTGACATTTGCTCTTACTAAAATTACCTTCTCCAAAAAACAACTTGTGCCTTTGCTTCTAATTCCAAAGGGTAAGGATCTTCAGTTTCTTGTTGATTTGATTAAGCAAGGAAAGATTAAAACAGTTATAGACTCCAAATTTCCTCTAGCTAAGGCTGAAGATGGTTGGTCTAAGAGTATTGATGTCATGCAACTGGGAAGATCATTTTTGAGTTTTAATTATGCTTATAGTGCTTTGTAA

Protein sequence

>MsG0780038417.01.T02

GIKLDGTSERKNILITAASGGVGHYAVQLAKLGNTHVTATCGARNMEIVKSLGADEVIDYKTPIGVELKSPSGKKYDAVVHCATDIAWSVFEPNLNTNGKVVDITPSSTSMLTFALTKITFSKKQLVPLLLIPKGKDLQFLVDLIKQGKIKTVIDSKFPLAKAEDGWSKSIDVMQLGRSFLSFNYAYSAL*

>MsG0780038417.01.T03

MVGLRVLMSCNWEDHF*

>MsG0780038417.01.T01

KILIKSIGIKLDGTSERKNILITAASGGVGHYAVQLAKLGNTHVTATCGARNMEIVKSLGADEVIDYKTPIGVELKSPSGKKYDAVVHCATDIAWSVFEPNLNTNGKVVDITPSSTSMLTFALTKITFSKKQLVPLLLIPKGKDLQFLVDLIKQGKIKTVIDSKFPLAKAEDGWSKSIDVMQLGRSFLSFNYAYSAL*