AlfalfaGEDB | MsG0180006004.01

M. sativa cultivar ZhongmuNo.1 / MsG0180006004.01

KEGG: -
GO: -
PFAM: PF04844

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006004.01.T01	XP_013470240.1	87.597	258	8	4	1	238	1	254	3.82E-131	380
MsG0180006004.01.T02	XP_013470240.1	92.248	258	9	4	1	251	1	254	5.39E-142	408

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006004.01.T01	Q9FMC8	47.584	269	88	11	1	229	1	256	2.57E-56	183
MsG0180006004.01.T02	Q9FMC8	50	272	90	10	1	242	1	256	4.71E-64	203

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006004.01.T01	A0A072VS70	87.597	258	8	4	1	238	1	254	1.83e-131	380
MsG0180006004.01.T02	A0A072VS70	92.248	258	9	4	1	251	1	254	2.58e-142	408

TFs/TRs:

Gene ID	Type	Classification
MsG0180006004.01.T02	TF	OFP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048849.01	MsG0180006004.01	0.808270	3.362744e-50	1.914398e-47
MsG0080048878.01	MsG0180006004.01	0.810729	9.971116e-51	6.057581e-48
MsG0180001610.01	MsG0180006004.01	0.801841	7.443810e-49	3.591426e-46
MsG0180003353.01	MsG0180006004.01	0.801902	7.231934e-49	3.494587e-46
MsG0180003697.01	MsG0180006004.01	0.808932	2.429397e-50	1.407109e-47
MsG0180004466.01	MsG0180006004.01	0.803924	2.763288e-49	1.406168e-46
MsG0180004750.01	MsG0180006004.01	0.814431	1.546345e-51	1.037410e-48
MsG0180006004.01	MsG0180006031.01	0.809273	2.053110e-50	1.200150e-47
MsG0180006004.01	MsG0280006711.01	-0.816790	4.611344e-52	3.299171e-49
MsG0180006004.01	MsG0280007647.01	0.822970	1.781585e-53	1.513884e-50
MsG0180006004.01	MsG0280008929.01	0.802382	5.761596e-49	2.818445e-46
MsG0180006004.01	MsG0380012122.01	0.800155	1.645635e-48	7.607420e-46
MsG0180006004.01	MsG0380016347.01	-0.821769	3.386003e-53	2.780530e-50
MsG0180006004.01	MsG0380016820.01	0.827020	1.969643e-54	1.878400e-51
MsG0180006004.01	MsG0480018911.01	0.808852	2.526450e-50	1.460257e-47
MsG0180006004.01	MsG0480020788.01	0.809163	2.167611e-50	1.263386e-47
MsG0180006004.01	MsG0480023073.01	0.827903	1.209667e-54	1.183719e-51
MsG0180006004.01	MsG0580024790.01	-0.804967	1.674808e-49	8.754473e-47
MsG0180006004.01	MsG0580026450.01	0.804817	1.800892e-49	9.377274e-47
MsG0180006004.01	MsG0580027103.01	0.801854	7.398470e-49	3.570722e-46
MsG0180006004.01	MsG0580028630.01	0.808701	2.721298e-50	1.566641e-47
MsG0180006004.01	MsG0780035960.01	0.805255	1.458413e-49	7.679154e-47
MsG0180006004.01	MsG0780036659.01	0.802229	6.194301e-49	3.018139e-46
MsG0180006004.01	MsG0780040422.01	0.800634	1.314382e-48	6.150682e-46
MsG0180006004.01	MsG0880044005.01	0.808092	3.670462e-50	2.079678e-47
MsG0180006004.01	MsG0880044142.01	0.811413	7.089071e-51	4.385365e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006004.01.T01	MTR_1g111640	87.597	258	8	4	1	238	1	254	4.63e-135	380
MsG0180006004.01.T01	MTR_7g105780	49.282	209	74	9	1	187	1	199	8.39e-50	164
MsG0180006004.01.T01	MTR_1g058000	36.957	230	95	8	6	206	1	209	4.38e-33	120
MsG0180006004.01.T01	MTR_1g072110	51.351	74	31	2	121	189	163	236	4.61e-16	75.1
MsG0180006004.01.T01	MTR_7g095150	49.315	73	31	2	121	187	128	200	2.24e-15	72.8
MsG0180006004.01.T01	MTR_2g102140	46.479	71	30	1	125	187	203	273	7.03e-12	64.3
MsG0180006004.01.T01	MTR_1g103640	34.906	106	55	3	96	187	74	179	9.49e-12	63.2
MsG0180006004.01.T02	MTR_1g111640	92.248	258	9	4	1	251	1	254	6.53e-146	408
MsG0180006004.01.T02	MTR_7g105780	51.628	215	73	9	1	200	1	199	2.34e-57	184
MsG0180006004.01.T02	MTR_1g058000	38.696	230	104	8	6	219	1	209	8.24e-38	132
MsG0180006004.01.T02	MTR_1g072110	51.351	74	31	2	134	202	163	236	5.75e-16	75.1
MsG0180006004.01.T02	MTR_7g095150	49.315	73	31	2	134	200	128	200	3.62e-15	72.4
MsG0180006004.01.T02	MTR_1g103640	34.906	106	55	3	109	200	74	179	9.52e-12	63.5
MsG0180006004.01.T02	MTR_2g102140	46.479	71	30	1	138	200	203	273	1.15e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006004.01.T01	AT5G04820	47.584	269	88	11	1	229	1	256	2.62e-57	183
MsG0180006004.01.T01	AT2G36050	48.718	117	42	5	82	188	66	174	3.56e-26	102
MsG0180006004.01.T01	AT3G52540	49.558	113	49	4	82	189	93	202	9.22e-23	94.0
MsG0180006004.01.T01	AT1G05420	44.444	63	31	1	129	187	172	234	3.15e-12	64.7
MsG0180006004.01.T01	AT1G05420	44.444	63	31	1	129	187	156	218	3.75e-12	64.3
MsG0180006004.01.T01	AT1G79960	40.449	89	47	3	105	187	172	260	4.86e-12	64.7
MsG0180006004.01.T02	AT5G04820	50.000	272	90	10	1	242	1	256	4.80e-65	203
MsG0180006004.01.T02	AT2G36050	48.718	117	42	5	95	201	66	174	5.26e-26	102
MsG0180006004.01.T02	AT3G52540	49.558	113	49	4	95	202	93	202	1.42e-22	94.0
MsG0180006004.01.T02	AT1G05420	44.444	63	31	1	142	200	172	234	4.77e-12	64.3
MsG0180006004.01.T02	AT1G05420	44.444	63	31	1	142	200	156	218	5.28e-12	63.9
MsG0180006004.01.T02	AT1G79960	40.449	89	47	3	118	200	172	260	7.94e-12	64.3

Find 33 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCTATAATGTTCTCTGTT+TGG	0.328276	1:+98687550	None:intergenic
AACACAACACTTTCTTTGAA+TGG	0.349626	1:+98687330	None:intergenic
AATAATCATGGCTTTATTGT+TGG	0.357962	1:-98687177	MsG0180006004.01.T02:CDS
CTACAACGACGGCGAATCAT+TGG	0.389604	1:-98687457	MsG0180006004.01.T02:CDS
GATCTCTTAAAATCTTCATA+TGG	0.409145	1:+98687288	None:intergenic
AGTCTCATGGTGTGAAAGAT+TGG	0.409364	1:-98687248	MsG0180006004.01.T02:CDS
GATGTTGTGAACCATGATTT+TGG	0.433746	1:+98687525	None:intergenic
TGTGAAAGATTGGGAAGGTT+TGG	0.450919	1:-98687238	MsG0180006004.01.T02:CDS
AGCTGGTATTTGAAAGTGAA+TGG	0.468308	1:-98687204	MsG0180006004.01.T02:CDS
TTCACCTTTGTATCTATGTG+AGG	0.483956	1:-98687049	MsG0180006004.01.T02:CDS
TGGCAATTCTTACCCTCTTG+TGG	0.498707	1:-98687654	MsG0180006004.01.T02:CDS
TAAGAGATCAATGGAAGAGA+TGG	0.508969	1:-98687274	MsG0180006004.01.T02:CDS
AGAAAGTGTTGTGTTAGCTA+TGG	0.521557	1:-98687322	MsG0180006004.01.T02:CDS
CACGAGTTCGATCTTAGAAA+AGG	0.522622	1:-98687370	MsG0180006004.01.T02:CDS
AGTTAAGTCGGAGAGGTCAT+CGG	0.526056	1:-98687418	MsG0180006004.01.T02:CDS
TGATGATGACATATTCAAAA+CGG	0.538193	1:-98687592	MsG0180006004.01.T02:CDS
TTATAGAAACACCAAAATCA+TGG	0.542649	1:-98687536	MsG0180006004.01.T02:CDS
TCACCTTTGTATCTATGTGA+GGG	0.550702	1:-98687048	MsG0180006004.01.T02:CDS
GTCTCATGGTGTGAAAGATT+GGG	0.558996	1:-98687247	MsG0180006004.01.T02:CDS
TTAGAAAAGGCGAAAGCGAA+TGG	0.571257	1:-98687357	MsG0180006004.01.T02:CDS
CACAAAGAAAACAACATCCA+TGG	0.572198	1:-98687674	MsG0180006004.01.T02:CDS
CGGCGAATCATTGGAGACAT+TGG	0.582049	1:-98687448	MsG0180006004.01.T02:CDS
CAAGAGGGTAAGAATTGCCA+TGG	0.585631	1:+98687657	None:intergenic
TGAGAAACTTGCAGATTCAG+AGG	0.589678	1:+98687503	None:intergenic
TAGATACAAAGGTGAAGAAG+AGG	0.592722	1:+98687056	None:intergenic
TGTGTTAGCTATGGAGTCAG+AGG	0.627955	1:-98687313	MsG0180006004.01.T02:CDS
TGTCCCTCACATAGATACAA+AGG	0.633534	1:+98687045	None:intergenic
GAAGAGATGGTTGAGTCTCA+TGG	0.637125	1:-98687261	MsG0180006004.01.T02:CDS
CATGGTGTGAAAGATTGGGA+AGG	0.649930	1:-98687243	MsG0180006004.01.T02:CDS
ATTGGTACGAGGAGTTAAGT+CGG	0.655000	1:-98687430	MsG0180006004.01.T02:CDS
GATTACTATTGCTACAACGA+CGG	0.655187	1:-98687468	MsG0180006004.01.T02:CDS
TACGAGGAGTTAAGTCGGAG+AGG	0.688804	1:-98687425	MsG0180006004.01.T02:CDS
TCATTGGAGACATTGGTACG+AGG	0.730029	1:-98687441	MsG0180006004.01.T02:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTCTTTGTGAAATTTCTT+TGG	+	Chr1:98687036-98687055	None:intergenic	20.0%
!	GATCTCTTAAAATCTTCATA+TGG	+	Chr1:98687434-98687453	None:intergenic	25.0%
!	GTGAATGGTAAAAATAATCA+TGG	-	Chr1:98687530-98687549	MsG0180006004.01.T02:CDS	25.0%
!	TTATAGAAACACCAAAATCA+TGG	-	Chr1:98687183-98687202	MsG0180006004.01.T02:CDS	25.0%
!	TTTCTATAATGTTCTCTGTT+TGG	+	Chr1:98687172-98687191	None:intergenic	25.0%
!!	TGATGATGACATATTCAAAA+CGG	-	Chr1:98687127-98687146	MsG0180006004.01.T02:CDS	25.0%
!!!	AATAATCATGGCTTTATTGT+TGG	-	Chr1:98687542-98687561	MsG0180006004.01.T02:CDS	25.0%
!!!	TGAAGATTTTAAGAGATCAA+TGG	-	Chr1:98687436-98687455	MsG0180006004.01.T02:CDS	25.0%
	AACACAACACTTTCTTTGAA+TGG	+	Chr1:98687392-98687411	None:intergenic	30.0%
!	ACTCGAAAAGAAAGAGTTTT+TGG	+	Chr1:98687095-98687114	None:intergenic	30.0%
!!	GAAAAGAAAGAGTTTTTGGA+TGG	+	Chr1:98687091-98687110	None:intergenic	30.0%
!!!	AAAACTCTTTCTTTTCGAGT+TGG	-	Chr1:98687095-98687114	MsG0180006004.01.T02:CDS	30.0%
!!!	TCTTTGGTTTTGAAAAGAGA+AGG	+	Chr1:98687020-98687039	None:intergenic	30.0%
	AGCTGGTATTTGAAAGTGAA+TGG	-	Chr1:98687515-98687534	MsG0180006004.01.T02:CDS	35.0%
	CACAAAGAAAACAACATCCA+TGG	-	Chr1:98687045-98687064	MsG0180006004.01.T02:CDS	35.0%
	GATTACTATTGCTACAACGA+CGG	-	Chr1:98687251-98687270	MsG0180006004.01.T02:CDS	35.0%
	TAAGAGATCAATGGAAGAGA+TGG	-	Chr1:98687445-98687464	MsG0180006004.01.T02:CDS	35.0%
	TAGATACAAAGGTGAAGAAG+AGG	+	Chr1:98687666-98687685	None:intergenic	35.0%
	TCACCTTTGTATCTATGTGA+GGG	-	Chr1:98687671-98687690	MsG0180006004.01.T02:CDS	35.0%
	TTCACCTTTGTATCTATGTG+AGG	-	Chr1:98687670-98687689	MsG0180006004.01.T02:CDS	35.0%
!	GATGTTGTGAACCATGATTT+TGG	+	Chr1:98687197-98687216	None:intergenic	35.0%
!!	AGAAAGTGTTGTGTTAGCTA+TGG	-	Chr1:98687397-98687416	MsG0180006004.01.T02:CDS	35.0%
	AGTCTCATGGTGTGAAAGAT+TGG	-	Chr1:98687471-98687490	MsG0180006004.01.T02:CDS	40.0%
	ATTGGTACGAGGAGTTAAGT+CGG	-	Chr1:98687289-98687308	MsG0180006004.01.T02:CDS	40.0%
	CACGAGTTCGATCTTAGAAA+AGG	-	Chr1:98687349-98687368	MsG0180006004.01.T02:CDS	40.0%
	GTCTCATGGTGTGAAAGATT+GGG	-	Chr1:98687472-98687491	MsG0180006004.01.T02:CDS	40.0%
	TGAGAAACTTGCAGATTCAG+AGG	+	Chr1:98687219-98687238	None:intergenic	40.0%
	TGTCCCTCACATAGATACAA+AGG	+	Chr1:98687677-98687696	None:intergenic	40.0%
	TGTGAAAGATTGGGAAGGTT+TGG	-	Chr1:98687481-98687500	MsG0180006004.01.T02:CDS	40.0%
	TTAGAAAAGGCGAAAGCGAA+TGG	-	Chr1:98687362-98687381	MsG0180006004.01.T02:CDS	40.0%
!!!	ACTCTTTCTTTTCGAGTTGG+TGG	-	Chr1:98687098-98687117	MsG0180006004.01.T02:CDS	40.0%
	AGTTAAGTCGGAGAGGTCAT+CGG	-	Chr1:98687301-98687320	MsG0180006004.01.T02:CDS	45.0%
	CAAGAGGGTAAGAATTGCCA+TGG	+	Chr1:98687065-98687084	None:intergenic	45.0%
	CATGGTGTGAAAGATTGGGA+AGG	-	Chr1:98687476-98687495	MsG0180006004.01.T02:CDS	45.0%
	GAAGAGATGGTTGAGTCTCA+TGG	-	Chr1:98687458-98687477	MsG0180006004.01.T02:CDS	45.0%
	TCATTGGAGACATTGGTACG+AGG	-	Chr1:98687278-98687297	MsG0180006004.01.T02:CDS	45.0%
	TGGCAATTCTTACCCTCTTG+TGG	-	Chr1:98687065-98687084	MsG0180006004.01.T02:CDS	45.0%
	TGTGTTAGCTATGGAGTCAG+AGG	-	Chr1:98687406-98687425	MsG0180006004.01.T02:CDS	45.0%
!	GTTTGGAAGAGCTTTTGAGC+TGG	-	Chr1:98687498-98687517	MsG0180006004.01.T02:CDS	45.0%
!!	AGTTTTTGGATGGCCACAAG+AGG	+	Chr1:98687081-98687100	None:intergenic	45.0%
!!	GTTTTTGGATGGCCACAAGA+GGG	+	Chr1:98687080-98687099	None:intergenic	45.0%
	CGGCGAATCATTGGAGACAT+TGG	-	Chr1:98687271-98687290	MsG0180006004.01.T02:CDS	50.0%
	CTACAACGACGGCGAATCAT+TGG	-	Chr1:98687262-98687281	MsG0180006004.01.T02:CDS	50.0%
	TACGAGGAGTTAAGTCGGAG+AGG	-	Chr1:98687294-98687313	MsG0180006004.01.T02:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	98686993	98687748	98686993	ID=MsG0180006004.01;Name=MsG0180006004.01
Chr1	mRNA	98686993	98687748	98686993	ID=MsG0180006004.01.T01;Parent=MsG0180006004.01;Name=MsG0180006004.01.T01;_AED=0.03;_eAED=0.03;_QI=0\|0\|0\|1\|0\|0\|2\|0\|238
Chr1	exon	98686993	98687573	98686993	ID=MsG0180006004.01.T01:exon:19774;Parent=MsG0180006004.01.T01
Chr1	exon	98687613	98687748	98687613	ID=MsG0180006004.01.T01:exon:19773;Parent=MsG0180006004.01.T01
Chr1	CDS	98687613	98687748	98687613	ID=MsG0180006004.01.T01:cds;Parent=MsG0180006004.01.T01
Chr1	CDS	98686993	98687573	98686993	ID=MsG0180006004.01.T01:cds;Parent=MsG0180006004.01.T01
Chr1	mRNA	98686993	98687748	98686993	ID=MsG0180006004.01.T02;Parent=MsG0180006004.01;Name=MsG0180006004.01.T02;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|251
Chr1	exon	98686993	98687748	98686993	ID=MsG0180006004.01.T02:exon:19775;Parent=MsG0180006004.01.T02
Chr1	CDS	98686993	98687748	98686993	ID=MsG0180006004.01.T02:cds;Parent=MsG0180006004.01.T02

Gene Sequence

>MsG0180006004.01.T01

ATGGGAAAGAAAAATTTGAAACTACCTTCTCTTTTCAAAACCAAAGAAATTTCACAAAGAAAACAACATCCATGGCAATTCTTACCCTCTTGTGGCCATCCAAAAACTCTTTCTTTTCGAGTTGGTGGTACTACTGACCAAACAGAGAACATTATAGAAACACCAAAATCATGGTTCACAACATCCTCTGAATCTGCAAGTTTCTCAACAGAATCAGAAGATTACTATTGCTACAACGACGGCGAATCATTGGAGACATTGGTACGAGGAGTTAAGTCGGAGAGGTCATCGGAGAGATTGTTTTTCGAAGCAGAAGACACGAGTTCGATCTTAGAAAAGGCGAAAGCGAATGGTTTTCCATTCAAAGAAAGTGTTGTGTTAGCTATGGAGTCAGAGGATCCATATGAAGATTTTAAGAGATCAATGGAAGAGATGGTTGAGTCTCATGGTGTGAAAGATTGGGAAGGTTTGGAAGAGCTTTTGAGCTGGTATTTGAAAGTGAATGGTAAAAATAATCATGGCTTTATTGTTGGTGCTTTTGTTGATTTGTTGATTAGTTTAGCAGCTTCTAATAATTCTAATAACTCTTGTTCTAATTTAACGTTATATTCTTCTGCTGTTTCTTCTTTTGCCTCTTCTTCACCTTTGTATCTATGTGAGGGACAGAATGAGATTACTGAGATTGAAGATGATAATAATAATGTAACAGCTTCTTAG

>MsG0180006004.01.T02

ATGGGAAAGAAAAATTTGAAACTACCTTCTCTTTTCAAAACCAAAGAAATTTCACAAAGAAAACAACATCCATGGCAATTCTTACCCTCTTGTGGCCATCCAAAAACTCTTTCTTTTCGAGTTGGTGGTACTACTGATGATGACATATTCAAAACGGTTAATTCTGTGTTTTTTGACCAAACAGAGAACATTATAGAAACACCAAAATCATGGTTCACAACATCCTCTGAATCTGCAAGTTTCTCAACAGAATCAGAAGATTACTATTGCTACAACGACGGCGAATCATTGGAGACATTGGTACGAGGAGTTAAGTCGGAGAGGTCATCGGAGAGATTGTTTTTCGAAGCAGAAGACACGAGTTCGATCTTAGAAAAGGCGAAAGCGAATGGTTTTCCATTCAAAGAAAGTGTTGTGTTAGCTATGGAGTCAGAGGATCCATATGAAGATTTTAAGAGATCAATGGAAGAGATGGTTGAGTCTCATGGTGTGAAAGATTGGGAAGGTTTGGAAGAGCTTTTGAGCTGGTATTTGAAAGTGAATGGTAAAAATAATCATGGCTTTATTGTTGGTGCTTTTGTTGATTTGTTGATTAGTTTAGCAGCTTCTAATAATTCTAATAACTCTTGTTCTAATTTAACGTTATATTCTTCTGCTGTTTCTTCTTTTGCCTCTTCTTCACCTTTGTATCTATGTGAGGGACAGAATGAGATTACTGAGATTGAAGATGATAATAATAATGTAACAGCTTCTTAG

Protein sequence

>MsG0180006004.01.T01

MGKKNLKLPSLFKTKEISQRKQHPWQFLPSCGHPKTLSFRVGGTTDQTENIIETPKSWFTTSSESASFSTESEDYYCYNDGESLETLVRGVKSERSSERLFFEAEDTSSILEKAKANGFPFKESVVLAMESEDPYEDFKRSMEEMVESHGVKDWEGLEELLSWYLKVNGKNNHGFIVGAFVDLLISLAASNNSNNSCSNLTLYSSAVSSFASSSPLYLCEGQNEITEIEDDNNNVTAS*

>MsG0180006004.01.T02

MGKKNLKLPSLFKTKEISQRKQHPWQFLPSCGHPKTLSFRVGGTTDDDIFKTVNSVFFDQTENIIETPKSWFTTSSESASFSTESEDYYCYNDGESLETLVRGVKSERSSERLFFEAEDTSSILEKAKANGFPFKESVVLAMESEDPYEDFKRSMEEMVESHGVKDWEGLEELLSWYLKVNGKNNHGFIVGAFVDLLISLAASNNSNNSCSNLTLYSSAVSSFASSSPLYLCEGQNEITEIEDDNNNVTAS*