AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180006209.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006209.01.T01 MTR_1g116080 65.487 339 20 5 1 298 39 321 3.30e-140 398
MsG0180006209.01.T01 MTR_1g116080 65.693 274 17 3 1 233 40 277 1.69e-113 330
MsG0180006209.01.T01 MTR_3g104850 28.195 266 122 6 23 288 96 292 1.22e-29 114
MsG0180006209.01.T01 MTR_3g104850 28.195 266 122 6 23 288 146 342 2.16e-29 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006209.01.T01 AT4G08790 49.702 336 68 7 1 292 29 307 9.48e-98 290
MsG0180006209.01.T01 AT5G12040 27.459 244 116 5 45 288 172 354 1.02e-27 110
MsG0180006209.01.T01 AT5G12040 32.075 159 69 4 45 203 172 291 2.18e-20 89.4

Find 75 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGAGAGCAGGTGGAGAAAT+TGG 0.233932 1:+101463426 None:intergenic
GCTTCTCTATAGTACTTAAT+TGG 0.237876 1:-101459687 MsG0180006209.01.T01:exon
ATCCTCAGGATAATCAAATA+AGG 0.266788 1:+101459585 None:intergenic
CTTGGAGGCTTTCAAGAAAA+AGG 0.272049 1:-101462659 MsG0180006209.01.T01:CDS
TTTCCTAGAAAGAACCTTAC+TGG 0.291096 1:-101461414 MsG0180006209.01.T01:intron
GACAGTCCTATTGGGCGTTT+GGG 0.332728 1:-101461159 MsG0180006209.01.T01:CDS
AGACAGTCCTATTGGGCGTT+TGG 0.340805 1:-101461160 MsG0180006209.01.T01:CDS
GGGGAAGGAGTGTGTATTAC+AGG 0.353654 1:-101460213 MsG0180006209.01.T01:CDS
CGATCCTTGGGGACTGTTGT+TGG 0.358659 1:-101460445 MsG0180006209.01.T01:CDS
CACATTGATTATCGATCCTT+GGG 0.362091 1:-101460457 MsG0180006209.01.T01:CDS
CAAATGATTACCTTAGCAAT+AGG 0.366454 1:+101459899 None:intergenic
AGGTTTGATGTGGATGTTCC+TGG 0.374846 1:-101461610 MsG0180006209.01.T01:CDS
ACCCCTTTGTGACTATTGAA+TGG 0.378528 1:+101459662 None:intergenic
GTTGCAGTAGACAGTCCTAT+TGG 0.386112 1:-101461168 MsG0180006209.01.T01:CDS
AAAGGAAGCCATTTGACTTC+TGG 0.391324 1:-101459716 MsG0180006209.01.T01:CDS
CTAAGGCCCAAACGCCCAAT+AGG 0.406011 1:+101461153 None:intergenic
GATGCAGAGAGTCAAGTATT+TGG 0.408938 1:-101462688 MsG0180006209.01.T01:intron
GTTTCAGATCGTTTGTCTAC+GGG 0.424933 1:-101459977 MsG0180006209.01.T01:intron
ACACATTGATTATCGATCCT+TGG 0.425567 1:-101460458 MsG0180006209.01.T01:CDS
CCCCTTTGTGACTATTGAAT+GGG 0.427806 1:+101459663 None:intergenic
TTGCAGTAGACAGTCCTATT+GGG 0.433163 1:-101461167 MsG0180006209.01.T01:CDS
CTGACTATGTCGTTTCTAAG+AGG 0.446212 1:+101461352 None:intergenic
AGTGACTGGTGAAGCACATT+GGG 0.449806 1:-101460901 MsG0180006209.01.T01:CDS
TGTTTCAGATCGTTTGTCTA+CGG 0.457934 1:-101459978 MsG0180006209.01.T01:intron
TACTTTGACAAGACGAGAGC+AGG 0.460604 1:+101463414 None:intergenic
TCCTCAGGATAATCAAATAA+GGG 0.460892 1:+101459586 None:intergenic
TTATTTGATTATCCTGAGGA+TGG 0.465319 1:-101459583 MsG0180006209.01.T01:three_prime_UTR
AGTATTTGGTTGTCACTTGG+AGG 0.473114 1:-101462674 MsG0180006209.01.T01:CDS
ACTGTTGTTGGTCGCTTACC+AGG 0.475755 1:-101460433 MsG0180006209.01.T01:intron
GGCAACTCGGACTGAATTAG+TGG 0.475966 1:+101463483 None:intergenic
ATGGCTTACCTCAGGGAGAT+GGG 0.487152 1:-101460233 MsG0180006209.01.T01:intron
TATGGCTTACCTCAGGGAGA+TGG 0.487745 1:-101460234 MsG0180006209.01.T01:intron
GCAGCTTTCCAGAAGTCAAA+TGG 0.488007 1:+101459708 None:intergenic
GTGCATTGTCCAGATACTAT+TGG 0.490967 1:-101460943 MsG0180006209.01.T01:intron
TCAAGTATTTGGTTGTCACT+TGG 0.491926 1:-101462677 MsG0180006209.01.T01:CDS
ACCCATTCAATAGTCACAAA+GGG 0.504051 1:-101459664 MsG0180006209.01.T01:three_prime_UTR
GATGTGGATGTTCCTGGTGA+AGG 0.505285 1:-101461604 MsG0180006209.01.T01:CDS
GTCATAGCTGCTGCACAAGC+TGG 0.511906 1:-101460513 MsG0180006209.01.T01:CDS
ATTGGAAGCGAGGTCGGTGA+TGG 0.517816 1:+101463444 None:intergenic
CAGTTGTTGCGTTTCCAACA+TGG 0.520574 1:-101461096 MsG0180006209.01.T01:CDS
CCACTTAGTGGGAAAAGGCC+TGG 0.521586 1:+101460177 None:intergenic
ACACCCCACTTAGTGGGAAA+AGG 0.529044 1:+101460172 None:intergenic
CGCAACAACTGATAAAGCTC+AGG 0.529790 1:+101461108 None:intergenic
ATGTGGATGTTCCTGGTGAA+GGG 0.539079 1:-101461603 MsG0180006209.01.T01:CDS
CTAATACCTGTGCTCCATGT+TGG 0.546597 1:+101461082 None:intergenic
TCATTTGAGCTGCGGCAACT+CGG 0.547963 1:+101463470 None:intergenic
TCGTTTGTCTACGGGAATTG+TGG 0.555672 1:-101459969 MsG0180006209.01.T01:CDS
TTACCTCAGGGAGATGGGGA+AGG 0.556193 1:-101460228 MsG0180006209.01.T01:intron
TCAAATCATAGCAAACACTA+AGG 0.560660 1:+101461136 None:intergenic
AGAGAAGATGCCTATTGCTA+AGG 0.563526 1:-101459909 MsG0180006209.01.T01:intron
AATGACAAAAGAGAAAGTTA+TGG 0.563585 1:-101460483 MsG0180006209.01.T01:CDS
GCGTTTCCAACATGGAGCAC+AGG 0.566309 1:-101461088 MsG0180006209.01.T01:intron
TCCCTTATTTGATTATCCTG+AGG 0.567134 1:-101459587 MsG0180006209.01.T01:three_prime_UTR
GGAGAAATTGGAAGCGAGGT+CGG 0.567271 1:+101463438 None:intergenic
TTCTTTATATACCCTTCACC+AGG 0.567718 1:+101461592 None:intergenic
GCAGCATTCACTAAAGTGAC+TGG 0.570698 1:-101460915 MsG0180006209.01.T01:CDS
ACATTGTATACACCATCCTC+AGG 0.580937 1:+101459571 None:intergenic
GTCACTTTAGTGAATGCTGC+GGG 0.582925 1:+101460918 None:intergenic
AAGTGACTGGTGAAGCACAT+TGG 0.586083 1:-101460902 MsG0180006209.01.T01:CDS
TGTAATACACCCCACTTAGT+GGG 0.589965 1:+101460166 None:intergenic
GACCCATTCAATAGTCACAA+AGG 0.598689 1:-101459665 MsG0180006209.01.T01:three_prime_UTR
CATGTTGTTGTAGATGACAC+TGG 0.606125 1:-101462608 MsG0180006209.01.T01:CDS
GAGGATGGTGTATACAATGT+TGG 0.606536 1:-101459568 MsG0180006209.01.T01:three_prime_UTR
GTAGTCTATTTCAATATGAG+AGG 0.614631 1:-101459639 MsG0180006209.01.T01:three_prime_UTR
TTTGACAAGACGAGAGCAGG+TGG 0.616133 1:+101463417 None:intergenic
TGGCTTACCTCAGGGAGATG+GGG 0.627146 1:-101460232 MsG0180006209.01.T01:intron
GAACCTTACTGGAAAATCAG+AGG 0.635204 1:-101461403 MsG0180006209.01.T01:CDS
GCGACCAACAACAGTCCCCA+AGG 0.638546 1:+101460441 None:intergenic
AGGTGGAGAAATTGGAAGCG+AGG 0.646802 1:+101463434 None:intergenic
AGTCACTTTAGTGAATGCTG+CGG 0.666530 1:+101460917 None:intergenic
TGTGTATTAAACAAATGTTG+AGG 0.680661 1:+101462629 None:intergenic
GATGGATGTCATTTGAGCTG+CGG 0.692126 1:+101463462 None:intergenic
CCCATTCAATAGTCACAAAG+GGG 0.696815 1:-101459663 MsG0180006209.01.T01:three_prime_UTR
ATGTTGTTGTAGATGACACT+GGG 0.710242 1:-101462607 MsG0180006209.01.T01:CDS
ACATTGATTATCGATCCTTG+GGG 0.710447 1:-101460456 MsG0180006209.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAATATATATATATA+AGG + Chr1:101459985-101459966 None:intergenic 0.0%
!! TAAATAAATAATTAATTAAA+AGG + Chr1:101459648-101459667 None:intergenic 0.0%
!!! ATTTATTTATTTGTTTTCAA+AGG - Chr1:101459663-101459682 MsG0180006209.01.T01:three_prime_UTR 10.0%
!!! TTTCAATTAATTTTATTTGA+AGG - Chr1:101461351-101461370 MsG0180006209.01.T01:intron 10.0%
!! AAAAATTTGAATAGTAAATG+TGG + Chr1:101462416-101462435 None:intergenic 15.0%
!! ATGTACCAAAATAAAAAATA+TGG - Chr1:101463368-101463387 MsG0180006209.01.T01:intron 15.0%
!! TAATTGAAAAACAATTAACT+GGG + Chr1:101461133-101461152 None:intergenic 15.0%
!! TGTACCAAAATAAAAAATAT+GGG - Chr1:101463369-101463388 MsG0180006209.01.T01:intron 15.0%
!! AACATATAGCATAAGTTATT+TGG + Chr1:101461272-101461291 None:intergenic 20.0%
!! AATTGAAAAACAATTAACTG+GGG + Chr1:101461132-101461151 None:intergenic 20.0%
!! ACATATAGCATAAGTTATTT+GGG + Chr1:101461271-101461290 None:intergenic 20.0%
!! ATAAAGAAGAGTAAAAAAGA+CGG + Chr1:101462967-101462986 None:intergenic 20.0%
!! CTAATTGAAAAACAATTAAC+TGG + Chr1:101461134-101461153 None:intergenic 20.0%
!! TTCATATCACATATAATTGA+AGG + Chr1:101461724-101461743 None:intergenic 20.0%
!!! AAAAATGAGAAGTTTTGATT+AGG + Chr1:101461495-101461514 None:intergenic 20.0%
!!! ATATATCAGCATATATTTTG+AGG - Chr1:101460171-101460190 MsG0180006209.01.T01:intron 20.0%
! AACACTTATCTATGATATGA+AGG - Chr1:101461747-101461766 MsG0180006209.01.T01:intron 25.0%
! AATGACAAAAGAGAAAGTTA+TGG - Chr1:101462498-101462517 MsG0180006209.01.T01:intron 25.0%
! ACCACTAAAAAAAACTTCAT+TGG + Chr1:101462872-101462891 None:intergenic 25.0%
! AGTAAGAATATACATGAAGA+TGG + Chr1:101462221-101462240 None:intergenic 25.0%
! ATTGAAAAACAATTAACTGG+GGG + Chr1:101461131-101461150 None:intergenic 25.0%
! GATCTTATATGATATTTCTC+AGG + Chr1:101462705-101462724 None:intergenic 25.0%
! GATGTATGCATCAATAATAA+AGG + Chr1:101463226-101463245 None:intergenic 25.0%
! GTAAGAATATACATGAAGAT+GGG + Chr1:101462220-101462239 None:intergenic 25.0%
! TCATAATAAGTCAATCCAAA+CGG - Chr1:101460099-101460118 MsG0180006209.01.T01:intron 25.0%
! TGTGTATTAAACAAATGTTG+AGG + Chr1:101460355-101460374 None:intergenic 25.0%
! TTATAGTATTAAGATCCGTT+TGG + Chr1:101460117-101460136 None:intergenic 25.0%
! TTCTTAATCTAATGTGTGTT+TGG - Chr1:101460956-101460975 MsG0180006209.01.T01:intron 25.0%
!! AGGATATTGTTTTCATATCT+TGG - Chr1:101461767-101461786 MsG0180006209.01.T01:intron 25.0%
!! ATCCTAAACAGCATATTTTA+AGG + Chr1:101462000-101462019 None:intergenic 25.0%
!! CAGTTAAAGTTTTCAAATGT+CGG - Chr1:101461603-101461622 MsG0180006209.01.T01:CDS 25.0%
!! GAAAGTAATTTTACAGAATC+AGG - Chr1:101461409-101461428 MsG0180006209.01.T01:CDS 25.0%
!! GATAAAGTTAATAGTCTTAC+AGG + Chr1:101460429-101460448 None:intergenic 25.0%
!! GTGTTTTCGGAAAAAAAAAA+GGG + Chr1:101461023-101461042 None:intergenic 25.0%
!! TCTTTCTGTTTGTCTTATTA+TGG - Chr1:101461315-101461334 MsG0180006209.01.T01:intron 25.0%
!! TGCTTTTCTGCTTAAAATAT+GGG - Chr1:101461932-101461951 MsG0180006209.01.T01:intron 25.0%
!! TGTGTTTTCGGAAAAAAAAA+AGG + Chr1:101461024-101461043 None:intergenic 25.0%
!!! AGAAGTTTTGATTAGGATTA+AGG + Chr1:101461488-101461507 None:intergenic 25.0%
!!! GAGTCCCATATTTTTTATTT+TGG + Chr1:101463376-101463395 None:intergenic 25.0%
!!! GTTTTTGGGATAAAGAATTA+GGG + Chr1:101461160-101461179 None:intergenic 25.0%
!!! TCCAATGAAGTTTTTTTTAG+TGG - Chr1:101462868-101462887 MsG0180006209.01.T01:intron 25.0%
!!! TCCTAAACAGCATATTTTAA+GGG + Chr1:101461999-101462018 None:intergenic 25.0%
!!! TGTTTTTGGGATAAAGAATT+AGG + Chr1:101461161-101461180 None:intergenic 25.0%
!!! TTGCTTTTCTGCTTAAAATA+TGG - Chr1:101461931-101461950 MsG0180006209.01.T01:intron 25.0%
!!! TTTTATTTGAAGGTTTGATG+TGG - Chr1:101461361-101461380 MsG0180006209.01.T01:CDS 25.0%
AACTCAATATTAACCGAGTA+AGG + Chr1:101460198-101460217 None:intergenic 30.0%
AATTTATCCTCAAAGCAGAA+AGG - Chr1:101460788-101460807 MsG0180006209.01.T01:intron 30.0%
ATCCTCAGGATAATCAAATA+AGG + Chr1:101463399-101463418 None:intergenic 30.0%
ATTTCACCCAAGAAAAATAG+AGG - Chr1:101461539-101461558 MsG0180006209.01.T01:intron 30.0%
ATTTGATAAAAGCCGTAACA+TGG - Chr1:101461105-101461124 MsG0180006209.01.T01:CDS 30.0%
CAAATGACAATGAATGTGTT+TGG + Chr1:101461090-101461109 None:intergenic 30.0%
CAAATGATTACCTTAGCAAT+AGG + Chr1:101463085-101463104 None:intergenic 30.0%
CACTCTAGCTGATTAAATTT+GGG + Chr1:101461192-101461211 None:intergenic 30.0%
GATTTAATGAATGCCTTATC+AGG - Chr1:101462446-101462465 MsG0180006209.01.T01:intron 30.0%
GCTTCTCTATAGTACTTAAT+TGG - Chr1:101463294-101463313 MsG0180006209.01.T01:intron 30.0%
GTAGTCTATTTCAATATGAG+AGG - Chr1:101463342-101463361 MsG0180006209.01.T01:intron 30.0%
TAAAAAAGACGGTATCATTG+TGG + Chr1:101462956-101462975 None:intergenic 30.0%
TAAGACAAACAGAAAGAAGT+TGG + Chr1:101461312-101461331 None:intergenic 30.0%
TAGGATATTTACTCTTGACA+TGG - Chr1:101461432-101461451 MsG0180006209.01.T01:intron 30.0%
TATGAGTCTGTTTGGATAAA+TGG - Chr1:101459856-101459875 MsG0180006209.01.T01:intron 30.0%
TCAAATCATAGCAAACACTA+AGG + Chr1:101461848-101461867 None:intergenic 30.0%
TCACTCTAGCTGATTAAATT+TGG + Chr1:101461193-101461212 None:intergenic 30.0%
TCCTCAGGATAATCAAATAA+GGG + Chr1:101463398-101463417 None:intergenic 30.0%
TTATTTGATTATCCTGAGGA+TGG - Chr1:101463398-101463417 MsG0180006209.01.T01:intron 30.0%
TTTCACCCAAGAAAAATAGA+GGG - Chr1:101461540-101461559 MsG0180006209.01.T01:intron 30.0%
! GTAATTTTACAGAATCAGGT+AGG - Chr1:101461413-101461432 MsG0180006209.01.T01:intron 30.0%
! TAGTTGGATTTACTGTTTTG+AGG + Chr1:101460741-101460760 None:intergenic 30.0%
!! CCTAAACAGCATATTTTAAG+GGG + Chr1:101461998-101462017 None:intergenic 30.0%
!! GAGTTGATAAACAAGTCAAT+AGG + Chr1:101461249-101461268 None:intergenic 30.0%
!! TATATTTTGAGGACCTTACT+CGG - Chr1:101460182-101460201 MsG0180006209.01.T01:CDS 30.0%
!! TTTCCATCTGAGTATTGATT+TGG - Chr1:101460464-101460483 MsG0180006209.01.T01:CDS 30.0%
!!! TTGGGTTTTGAGTTGTTTTT+GGG + Chr1:101461174-101461193 None:intergenic 30.0%
!!! TTTGGGTTTTGAGTTGTTTT+TGG + Chr1:101461175-101461194 None:intergenic 30.0%
AAATGATCCTTTCTGCTTTG+AGG + Chr1:101460798-101460817 None:intergenic 35.0%
AACAAGTATTACCGCTATAG+CGG - Chr1:101461221-101461240 MsG0180006209.01.T01:intron 35.0%
AACATAAGCCTTTGTAAAGG+TGG + Chr1:101460066-101460085 None:intergenic 35.0%
AAGATCATATGTATGAGCTG+AGG - Chr1:101462719-101462738 MsG0180006209.01.T01:intron 35.0%
ACACCAAATCAATACTCAGA+TGG + Chr1:101460470-101460489 None:intergenic 35.0%
ACATGGTATCAAGGCTAAAT+GGG - Chr1:101461449-101461468 MsG0180006209.01.T01:intron 35.0%
ACCAATACTAGAGAATGGAA+TGG - Chr1:101463124-101463143 MsG0180006209.01.T01:intron 35.0%
ACCCATTCAATAGTCACAAA+GGG - Chr1:101463317-101463336 MsG0180006209.01.T01:intron 35.0%
ACTATGAGCTTATTGACGTA+AGG + Chr1:101459923-101459942 None:intergenic 35.0%
AGAATAAAAGCAAGCAAACC+TGG + Chr1:101459820-101459839 None:intergenic 35.0%
AGTTGTTAGAAACGTTAGTG+TGG + Chr1:101462388-101462407 None:intergenic 35.0%
ATCATCTCCAGTGAAAAAAC+TGG - Chr1:101461960-101461979 MsG0180006209.01.T01:intron 35.0%
ATGTTGTTGTAGATGACACT+GGG - Chr1:101460374-101460393 MsG0180006209.01.T01:intron 35.0%
GCTAACATAAGCCTTTGTAA+AGG + Chr1:101460069-101460088 None:intergenic 35.0%
GCTGATATATAGACACTGTT+TGG + Chr1:101460161-101460180 None:intergenic 35.0%
GGAGAGTTGAAGAAAGTAAT+TGG + Chr1:101462367-101462386 None:intergenic 35.0%
GTATTAGTATTGCTCAACCT+TGG - Chr1:101459761-101459780 MsG0180006209.01.T01:intron 35.0%
GTTCATGATATGAGTCTGTT+TGG - Chr1:101459848-101459867 MsG0180006209.01.T01:intron 35.0%
TCAAGTATTTGGTTGTCACT+TGG - Chr1:101460304-101460323 MsG0180006209.01.T01:intron 35.0%
TCCATTCCATTCTCTAGTAT+TGG + Chr1:101463128-101463147 None:intergenic 35.0%
TGCATGAGAAGAATGAGAAA+TGG - Chr1:101460640-101460659 MsG0180006209.01.T01:intron 35.0%
TGTTTCAGATCGTTTGTCTA+CGG - Chr1:101463003-101463022 MsG0180006209.01.T01:intron 35.0%
TTAACCAACCACCTTTACAA+AGG - Chr1:101460055-101460074 MsG0180006209.01.T01:intron 35.0%
TTACGAGGAAGAAAGAAACA+AGG + Chr1:101462657-101462676 None:intergenic 35.0%
TTACTCTTGACATGGTATCA+AGG - Chr1:101461440-101461459 MsG0180006209.01.T01:intron 35.0%
TTCTGCTTAAAATATGGGAG+TGG - Chr1:101461937-101461956 MsG0180006209.01.T01:intron 35.0%
TTCTTTATATACCCTTCACC+AGG + Chr1:101461392-101461411 None:intergenic 35.0%
TTGAAGGAAACGTATATCGT+TGG + Chr1:101461708-101461727 None:intergenic 35.0%
TTTCCAGTAAGGTTCTTTCT+AGG + Chr1:101461573-101461592 None:intergenic 35.0%
TTTCCTAGAAAGAACCTTAC+TGG - Chr1:101461567-101461586 MsG0180006209.01.T01:intron 35.0%
! AAAATGTTGCTTCTGTGTCA+TGG + Chr1:101460931-101460950 None:intergenic 35.0%
! ACACATTGATTATCGATCCT+TGG - Chr1:101462523-101462542 MsG0180006209.01.T01:intron 35.0%
! ACATTGATTATCGATCCTTG+GGG - Chr1:101462525-101462544 MsG0180006209.01.T01:intron 35.0%
! AGTAAGGTTCTTTCTAGGAA+AGG + Chr1:101461568-101461587 None:intergenic 35.0%
! AGTTGGTTTCTGATTTCAAG+AGG + Chr1:101461295-101461314 None:intergenic 35.0%
! CACATTGATTATCGATCCTT+GGG - Chr1:101462524-101462543 MsG0180006209.01.T01:intron 35.0%
! CAGGTGTATTTTTCTGTAAG+TGG + Chr1:101460410-101460429 None:intergenic 35.0%
! CCCCTTAAAATATGCTGTTT+AGG - Chr1:101461995-101462014 MsG0180006209.01.T01:intron 35.0%
! GAAGTATCAAGTTTCTTACC+TGG + Chr1:101462569-101462588 None:intergenic 35.0%
! TCCCTTATTTGATTATCCTG+AGG - Chr1:101463394-101463413 MsG0180006209.01.T01:intron 35.0%
!! AAACAGCATATTTTAAGGGG+AGG + Chr1:101461995-101462014 None:intergenic 35.0%
!! AAGGTTCCCTCTATTTTTCT+TGG + Chr1:101461549-101461568 None:intergenic 35.0%
!! AGGTTCCCTCTATTTTTCTT+GGG + Chr1:101461548-101461567 None:intergenic 35.0%
!! CACTTTTGACTATCATTACG+AGG + Chr1:101462672-101462691 None:intergenic 35.0%
!! TTCCATTCTCTAGTATTGGT+CGG + Chr1:101463124-101463143 None:intergenic 35.0%
!!! ATTGTCTCCAGTTTTTTCAC+TGG + Chr1:101461970-101461989 None:intergenic 35.0%
!!! CATATCTTGGTGATGTTTTC+AGG - Chr1:101461780-101461799 MsG0180006209.01.T01:intron 35.0%
AACCTTGGATGGACCTATTA+TGG - Chr1:101459776-101459795 MsG0180006209.01.T01:intron 40.0%
AAGATGGGAATGATGAAAGC+AGG + Chr1:101462205-101462224 None:intergenic 40.0%
ACATAAGAGCTTATAGCACG+AGG - Chr1:101459897-101459916 MsG0180006209.01.T01:intron 40.0%
ACATTGTATACACCATCCTC+AGG + Chr1:101463413-101463432 None:intergenic 40.0%
ACCCCTTTGTGACTATTGAA+TGG + Chr1:101463322-101463341 None:intergenic 40.0%
ACGAAGAAGTAGAGTAAGAG+AGG - Chr1:101460612-101460631 MsG0180006209.01.T01:intron 40.0%
ACGGTTGGAACAAGAGATTA+TGG - Chr1:101460587-101460606 MsG0180006209.01.T01:intron 40.0%
AGAAGAATGAGAAATGGAGC+GGG - Chr1:101460646-101460665 MsG0180006209.01.T01:intron 40.0%
AGAGAAGATGCCTATTGCTA+AGG - Chr1:101463072-101463091 MsG0180006209.01.T01:intron 40.0%
ATCCAACTACTCTGTGTGTT+TGG - Chr1:101460752-101460771 MsG0180006209.01.T01:intron 40.0%
CATGTTGTTGTAGATGACAC+TGG - Chr1:101460373-101460392 MsG0180006209.01.T01:intron 40.0%
CCCATTCAATAGTCACAAAG+GGG - Chr1:101463318-101463337 MsG0180006209.01.T01:intron 40.0%
CCCCTTTGTGACTATTGAAT+GGG + Chr1:101463321-101463340 None:intergenic 40.0%
CGAAGAAGTAGAGTAAGAGA+GGG - Chr1:101460613-101460632 MsG0180006209.01.T01:intron 40.0%
CTTCCTTTGCTGAAACGAAA+AGG + Chr1:101463253-101463272 None:intergenic 40.0%
CTTGGAGGCTTTCAAGAAAA+AGG - Chr1:101460322-101460341 MsG0180006209.01.T01:intron 40.0%
GAACCTTACTGGAAAATCAG+AGG - Chr1:101461578-101461597 MsG0180006209.01.T01:CDS 40.0%
GACATGGTATCAAGGCTAAA+TGG - Chr1:101461448-101461467 MsG0180006209.01.T01:intron 40.0%
GACCCATTCAATAGTCACAA+AGG - Chr1:101463316-101463335 MsG0180006209.01.T01:intron 40.0%
GAGAAGAATGAGAAATGGAG+CGG - Chr1:101460645-101460664 MsG0180006209.01.T01:intron 40.0%
GAGGAAGAAAGAAACAAGGA+AGG + Chr1:101462653-101462672 None:intergenic 40.0%
GAGGATGGTGTATACAATGT+TGG - Chr1:101463413-101463432 MsG0180006209.01.T01:CDS 40.0%
GATGCAGAGAGTCAAGTATT+TGG - Chr1:101460293-101460312 MsG0180006209.01.T01:intron 40.0%
GCAACACAAACGCAAGTAAA+CGG - Chr1:101460568-101460587 MsG0180006209.01.T01:intron 40.0%
GTGCATTGTCCAGATACTAT+TGG - Chr1:101462038-101462057 MsG0180006209.01.T01:intron 40.0%
GTTTCAGATCGTTTGTCTAC+GGG - Chr1:101463004-101463023 MsG0180006209.01.T01:intron 40.0%
TAAGCCTTTGTAAAGGTGGT+TGG + Chr1:101460062-101460081 None:intergenic 40.0%
TAGTATTGCTCAACCTTGGA+TGG - Chr1:101459765-101459784 MsG0180006209.01.T01:intron 40.0%
TCCAACTACTCTGTGTGTTT+GGG - Chr1:101460753-101460772 MsG0180006209.01.T01:intron 40.0%
TGTAATACACCCCACTTAGT+GGG + Chr1:101462818-101462837 None:intergenic 40.0%
TTGCAGTAGACAGTCCTATT+GGG - Chr1:101461814-101461833 MsG0180006209.01.T01:intron 40.0%
! AAAGGAAGCCATTTGACTTC+TGG - Chr1:101463265-101463284 MsG0180006209.01.T01:intron 40.0%
! AGTATTTGGTTGTCACTTGG+AGG - Chr1:101460307-101460326 MsG0180006209.01.T01:intron 40.0%
! AGTCACTTTAGTGAATGCTG+CGG + Chr1:101462067-101462086 None:intergenic 40.0%
! CTGACTATGTCGTTTCTAAG+AGG + Chr1:101461632-101461651 None:intergenic 40.0%
! GAACCTCTGATTTTCCAGTA+AGG + Chr1:101461584-101461603 None:intergenic 40.0%
! GGAGCTCTCAATTGTGTTTT+CGG + Chr1:101461036-101461055 None:intergenic 40.0%
! GTACCTTTTCGTTTCAGCAA+AGG - Chr1:101463247-101463266 MsG0180006209.01.T01:intron 40.0%
! TCTTTTGTGGGTGCTAAAGA+TGG - Chr1:101459733-101459752 MsG0180006209.01.T01:intron 40.0%
!! CCTGAAGCATTCTCTTTTGT+GGG - Chr1:101459721-101459740 MsG0180006209.01.T01:intron 40.0%
!! GAGCAGTATTGGTCCATAAT+AGG + Chr1:101459792-101459811 None:intergenic 40.0%
!! TCCATTCTCTAGTATTGGTC+GGG + Chr1:101463123-101463142 None:intergenic 40.0%
!! TCCTGAAGCATTCTCTTTTG+TGG - Chr1:101459720-101459739 MsG0180006209.01.T01:CDS 40.0%
AACAGTTTGAATCTCCAGCG+AGG + Chr1:101462906-101462925 None:intergenic 45.0%
AAGAATGAGAAATGGAGCGG+GGG - Chr1:101460648-101460667 MsG0180006209.01.T01:intron 45.0%
AAGAGCTCCTAACCTCTGTA+TGG + Chr1:101461057-101461076 None:intergenic 45.0%
AAGTGACTGGTGAAGCACAT+TGG - Chr1:101462079-101462098 MsG0180006209.01.T01:intron 45.0%
ACAGGACAGCAAAAACAACC+AGG - Chr1:101462786-101462805 MsG0180006209.01.T01:intron 45.0%
ACCAATACTGCTCTTTAGCC+AGG - Chr1:101459799-101459818 MsG0180006209.01.T01:intron 45.0%
ACCCAAACACACAGAGTAGT+TGG + Chr1:101460757-101460776 None:intergenic 45.0%
ACCCGACCAATACTAGAGAA+TGG - Chr1:101463119-101463138 MsG0180006209.01.T01:intron 45.0%
ACCTGGCTAAAGAGCAGTAT+TGG + Chr1:101459803-101459822 None:intergenic 45.0%
AGGTTTGATGTGGATGTTCC+TGG - Chr1:101461371-101461390 MsG0180006209.01.T01:CDS 45.0%
AGTGACTGGTGAAGCACATT+GGG - Chr1:101462080-101462099 MsG0180006209.01.T01:intron 45.0%
ATGTGGATGTTCCTGGTGAA+GGG - Chr1:101461378-101461397 MsG0180006209.01.T01:CDS 45.0%
ATTAACTGGGGGCCATGTTA+CGG + Chr1:101461120-101461139 None:intergenic 45.0%
CAATTGAGAGCTCCATACAG+AGG - Chr1:101461042-101461061 MsG0180006209.01.T01:intron 45.0%
CACAAACGCAAGTAAACGGT+TGG - Chr1:101460572-101460591 MsG0180006209.01.T01:intron 45.0%
CACTAAGTGGGGTGTATTAC+AGG - Chr1:101462817-101462836 MsG0180006209.01.T01:intron 45.0%
CAGTTGTTGCGTTTCCAACA+TGG - Chr1:101461885-101461904 MsG0180006209.01.T01:intron 45.0%
CCCACAAAAGAGAATGCTTC+AGG + Chr1:101459724-101459743 None:intergenic 45.0%
CCTAGCAATGCTTCTCTTCT+CGG - Chr1:101462163-101462182 MsG0180006209.01.T01:intron 45.0%
CGCAACAACTGATAAAGCTC+AGG + Chr1:101461876-101461895 None:intergenic 45.0%
CTAATACCTGTGCTCCATGT+TGG + Chr1:101461902-101461921 None:intergenic 45.0%
CTGTAATACACCCCACTTAG+TGG + Chr1:101462819-101462838 None:intergenic 45.0%
GAAGAATGAGAAATGGAGCG+GGG - Chr1:101460647-101460666 MsG0180006209.01.T01:intron 45.0%
GCAGCATTCACTAAAGTGAC+TGG - Chr1:101462066-101462085 MsG0180006209.01.T01:intron 45.0%
GCAGCTTTCCAGAAGTCAAA+TGG + Chr1:101463276-101463295 None:intergenic 45.0%
GTCCATAATAGGTCCATCCA+AGG + Chr1:101459781-101459800 None:intergenic 45.0%
GTTGCAGTAGACAGTCCTAT+TGG - Chr1:101461813-101461832 MsG0180006209.01.T01:intron 45.0%
TACTTTGACAAGACGAGAGC+AGG + Chr1:101459570-101459589 None:intergenic 45.0%
TCGTTTGTCTACGGGAATTG+TGG - Chr1:101463012-101463031 MsG0180006209.01.T01:intron 45.0%
! GATGGATGTCATTTGAGCTG+CGG + Chr1:101459522-101459541 None:intergenic 45.0%
! GTCACTTTAGTGAATGCTGC+GGG + Chr1:101462066-101462085 None:intergenic 45.0%
!!! CTTGGTGATGTTTTCAGGCA+AGG - Chr1:101461785-101461804 MsG0180006209.01.T01:intron 45.0%
ACACCCCACTTAGTGGGAAA+AGG + Chr1:101462812-101462831 None:intergenic 50.0%
ACGAGAGCAGGTGGAGAAAT+TGG + Chr1:101459558-101459577 None:intergenic 50.0%
ACTGTTGTTGGTCGCTTACC+AGG - Chr1:101462548-101462567 MsG0180006209.01.T01:intron 50.0%
AGACAGTCCTATTGGGCGTT+TGG - Chr1:101461821-101461840 MsG0180006209.01.T01:intron 50.0%
AGGTGGAGAAATTGGAAGCG+AGG + Chr1:101459550-101459569 None:intergenic 50.0%
ATGGCTTACCTCAGGGAGAT+GGG - Chr1:101462748-101462767 MsG0180006209.01.T01:intron 50.0%
GACAGTCCTATTGGGCGTTT+GGG - Chr1:101461822-101461841 MsG0180006209.01.T01:intron 50.0%
GAGAGCTCCATACAGAGGTT+AGG - Chr1:101461047-101461066 MsG0180006209.01.T01:intron 50.0%
GATGTGGATGTTCCTGGTGA+AGG - Chr1:101461377-101461396 MsG0180006209.01.T01:CDS 50.0%
GCAGCAGCTATGACCTGATA+AGG + Chr1:101462462-101462481 None:intergenic 50.0%
GCCTCATATGGCTTACCTCA+GGG - Chr1:101462741-101462760 MsG0180006209.01.T01:intron 50.0%
GGAGAAATTGGAAGCGAGGT+CGG + Chr1:101459546-101459565 None:intergenic 50.0%
GGCAACTCGGACTGAATTAG+TGG + Chr1:101459501-101459520 None:intergenic 50.0%
GGTGATTTCATTCTCCTCGC+TGG - Chr1:101462889-101462908 MsG0180006209.01.T01:intron 50.0%
GTATGAGCTGAGGCCTCATA+TGG - Chr1:101462729-101462748 MsG0180006209.01.T01:intron 50.0%
GTCAATAGGCTCCGCTATAG+CGG + Chr1:101461235-101461254 None:intergenic 50.0%
GTGGGGTGTATTACAGGAGA+AGG - Chr1:101462823-101462842 MsG0180006209.01.T01:intron 50.0%
TATGGCTTACCTCAGGGAGA+TGG - Chr1:101462747-101462766 MsG0180006209.01.T01:intron 50.0%
TCAAAGGAAGCTGCATCAGC+TGG - Chr1:101459679-101459698 MsG0180006209.01.T01:exon 50.0%
TCATTTGAGCTGCGGCAACT+CGG + Chr1:101459514-101459533 None:intergenic 50.0%
TCCCTGAGGTAAGCCATATG+AGG + Chr1:101462745-101462764 None:intergenic 50.0%
TTTGACAAGACGAGAGCAGG+TGG + Chr1:101459567-101459586 None:intergenic 50.0%
! AGGCCTTTTCCCACTAAGTG+GGG - Chr1:101462806-101462825 MsG0180006209.01.T01:intron 50.0%
! CAGGCCTTTTCCCACTAAGT+GGG - Chr1:101462805-101462824 MsG0180006209.01.T01:intron 50.0%
! GGGGAAGGAGTGTGTATTAC+AGG - Chr1:101462768-101462787 MsG0180006209.01.T01:intron 50.0%
!! CCGAGAAGAGAAGCATTGCT+AGG + Chr1:101462166-101462185 None:intergenic 50.0%
AATGAGAAATGGAGCGGGGG+TGG - Chr1:101460651-101460670 MsG0180006209.01.T01:intron 55.0%
ATGAGAAATGGAGCGGGGGT+GGG - Chr1:101460652-101460671 MsG0180006209.01.T01:intron 55.0%
ATTGGAAGCGAGGTCGGTGA+TGG + Chr1:101459540-101459559 None:intergenic 55.0%
CCACTTAGTGGGAAAAGGCC+TGG + Chr1:101462807-101462826 None:intergenic 55.0%
CGATCCTTGGGGACTGTTGT+TGG - Chr1:101462536-101462555 MsG0180006209.01.T01:intron 55.0%
CTAAGGCCCAAACGCCCAAT+AGG + Chr1:101461831-101461850 None:intergenic 55.0%
CTGCGGGCACCAATAGTATC+TGG + Chr1:101462050-101462069 None:intergenic 55.0%
GCGTTTCCAACATGGAGCAC+AGG - Chr1:101461893-101461912 MsG0180006209.01.T01:intron 55.0%
GGCCTCATATGGCTTACCTC+AGG - Chr1:101462740-101462759 MsG0180006209.01.T01:intron 55.0%
GTCATAGCTGCTGCACAAGC+TGG - Chr1:101462468-101462487 MsG0180006209.01.T01:intron 55.0%
TGGCTTACCTCAGGGAGATG+GGG - Chr1:101462749-101462768 MsG0180006209.01.T01:intron 55.0%
TTACCTCAGGGAGATGGGGA+AGG - Chr1:101462753-101462772 MsG0180006209.01.T01:intron 55.0%
! CCAGGCCTTTTCCCACTAAG+TGG - Chr1:101462804-101462823 MsG0180006209.01.T01:intron 55.0%
ACTCCTTCCCCATCTCCCTG+AGG + Chr1:101462759-101462778 None:intergenic 60.0%
GCGACCAACAACAGTCCCCA+AGG + Chr1:101462543-101462562 None:intergenic 60.0%
TGAGAAATGGAGCGGGGGTG+GGG - Chr1:101460653-101460672 MsG0180006209.01.T01:intron 60.0%
GAGAAATGGAGCGGGGGTGG+GGG - Chr1:101460654-101460673 MsG0180006209.01.T01:intron 65.0%
AATGGAGCGGGGGTGGGGGT+TGG - Chr1:101460658-101460677 MsG0180006209.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 101459494 101463509 101459494 ID=MsG0180006209.01;Name=MsG0180006209.01
Chr1 mRNA 101459494 101463509 101459494 ID=MsG0180006209.01.T01;Parent=MsG0180006209.01;Name=MsG0180006209.01.T01;_AED=0.37;_eAED=0.43;_QI=0|0.44|0.6|1|0.44|0.3|10|204|298
Chr1 exon 101459494 101459739 101459494 ID=MsG0180006209.01.T01:exon:42466;Parent=MsG0180006209.01.T01
Chr1 exon 101459910 101459992 101459910 ID=MsG0180006209.01.T01:exon:42465;Parent=MsG0180006209.01.T01
Chr1 exon 101460181 101460241 101460181 ID=MsG0180006209.01.T01:exon:42464;Parent=MsG0180006209.01.T01
Chr1 exon 101460434 101460535 101460434 ID=MsG0180006209.01.T01:exon:42463;Parent=MsG0180006209.01.T01
Chr1 exon 101460860 101460952 101460860 ID=MsG0180006209.01.T01:exon:42462;Parent=MsG0180006209.01.T01
Chr1 exon 101461089 101461234 101461089 ID=MsG0180006209.01.T01:exon:42461;Parent=MsG0180006209.01.T01
Chr1 exon 101461353 101461428 101461353 ID=MsG0180006209.01.T01:exon:42460;Parent=MsG0180006209.01.T01
Chr1 exon 101461573 101461630 101461573 ID=MsG0180006209.01.T01:exon:42459;Parent=MsG0180006209.01.T01
Chr1 exon 101462575 101462703 101462575 ID=MsG0180006209.01.T01:exon:42458;Parent=MsG0180006209.01.T01
Chr1 exon 101463403 101463509 101463403 ID=MsG0180006209.01.T01:exon:42457;Parent=MsG0180006209.01.T01
Chr1 CDS 101463403 101463509 101463403 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 CDS 101462575 101462703 101462575 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 CDS 101461573 101461630 101461573 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 CDS 101461353 101461428 101461353 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 CDS 101461089 101461234 101461089 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 CDS 101460860 101460952 101460860 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 CDS 101460434 101460535 101460434 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 CDS 101460181 101460241 101460181 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 CDS 101459910 101459992 101459910 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 CDS 101459698 101459739 101459698 ID=MsG0180006209.01.T01:cds;Parent=MsG0180006209.01.T01
Chr1 three_prime_UTR 101459494 101459697 101459494 ID=MsG0180006209.01.T01:three_prime_utr;Parent=MsG0180006209.01.T01
Gene Sequence

>MsG0180006209.01.T01

ATGGCCACTAATTCAGTCCGAGTTGCCGCAGCTCAAATGACATCCATCACCGACCTCGCTTCCAATTTCTCCACCTGCTCTCGTCTTGTCAAAGTAACTACTGTACTAGAGTCAAGTATTTGGTTGTCACTTGGAGGCTTTCAAGAAAAAGGATCTGATCCTCAACATTTGTTTAATACACATGTTGTTGTAGATGACACTGGGAAAATTCAAACCACTTACAGAAAAATACACCTGTTTGATGTGGATGTTCCTGGTGAAGGGTATATAAAGAAAGTAATTTTACAGAATCAGAAAGAACCTTACTGGAAAATCAGAGGTTCAGTTAAAGTTTTCAAATGTCGGCATCCTCTTAGAAACGACATAGTCAGATATTGTTTTCATATCTTGGTGATGTTTTCAGGCAAGGATATTGTTGCAGTAGACAGTCCTATTGGGCGTTTGGGCCTTAGTGTTTGCTATGATTTGAGATTTCCTGAGCTTTATCAGTTGTTGCGTTTCCAACATGGAGCACAGATACTATTGGTGCCCGCAGCATTCACTAAAGTGACTGGTGAAGCACATTGGGAGATTCTTCTTCGTGCTCGTGCAATCGAGAATCAATGCTATGTCATAGCTGCTGCACAAGCTGGCACACACAATGACAAAAGAGAAAGTTATGGTGACACATTGATTATCGATCCTTGGGGACTGTTGTTGGTCGCTTACCAGGGAGATGGGGAAGGAGTGTGTATTACAGGACAGCAAAAACAACCAGGCCTTTTCCCACTAAATCGTTTGTCTACGGGAATTGTGGTAGCTGATATTGATTTATCATTAGTTGATTCAGTAAGAGAGAAGATGCCTATTGCTAAGCAAAGGAAGCCATTTGACTTCTGGAAAGCTGCTTCTCTATAG
Protein sequence

>MsG0180006209.01.T01

MATNSVRVAAAQMTSITDLASNFSTCSRLVKVTTVLESSIWLSLGGFQEKGSDPQHLFNTHVVVDDTGKIQTTYRKIHLFDVDVPGEGYIKKVILQNQKEPYWKIRGSVKVFKCRHPLRNDIVRYCFHILVMFSGKDIVAVDSPIGRLGLSVCYDLRFPELYQLLRFQHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGLLLVAYQGDGEGVCITGQQKQPGLFPLNRLSTGIVVADIDLSLVDSVREKMPIAKQRKPFDFWKAASL*