AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046288.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046288.01.T01 MTR_4g104870 98.095 105 2 0 1 105 1 105 9.96e-76 220
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046288.01.T01 AT4G24700 41.860 86 42 3 16 98 24 104 7.97e-14 63.5

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCAACACTTCTAAATCT+TGG 0.352567 8:-71552565 None:intergenic
GTTTCAAGAAGGATTGAAAC+AGG 0.360068 8:+71552791 MsG0880046288.01.T01:CDS
GTTTCAAGCTTGAACCTTGA+AGG 0.364845 8:-71552674 None:intergenic
ACACTTCTAAATCTTGGTTA+TGG 0.368826 8:-71552559 None:intergenic
GGAGACACAAAATCATCAAA+TGG 0.381912 8:-71552830 None:intergenic
GAAACTGTTACAAGCTCATA+AGG 0.391839 8:-71552773 None:intergenic
TGACTTTCAACACTCACTTT+GGG 0.415015 8:+71552748 MsG0880046288.01.T01:CDS
ATTTCAGAGGTAAGCCTTCA+AGG 0.422597 8:+71552660 MsG0880046288.01.T01:CDS
GTGACTTTCAACACTCACTT+TGG 0.455830 8:+71552747 MsG0880046288.01.T01:CDS
GAGACACAAAATCATCAAAT+GGG 0.479325 8:-71552829 None:intergenic
TTCCCTAGTTGATGATGACT+TGG 0.515303 8:-71552702 None:intergenic
AGCTTGTAACAGTTTCAAGA+AGG 0.526241 8:+71552780 MsG0880046288.01.T01:CDS
TAACCAAGTCATCATCAACT+AGG 0.529261 8:+71552699 MsG0880046288.01.T01:CDS
AAACTGTTACAAGCTCATAA+GGG 0.547351 8:-71552772 None:intergenic
ACTCAAAGGCTTTACACTGC+CGG 0.552991 8:-71552615 None:intergenic
AACCAAGTCATCATCAACTA+GGG 0.574505 8:+71552700 MsG0880046288.01.T01:CDS
TGAAAGCTGTTGCACAAGCT+TGG 0.604295 8:+71552582 MsG0880046288.01.T01:CDS
CGCGCGTCGAATTCACTCAA+AGG 0.612095 8:-71552629 None:intergenic
TTTCAAGAAGGATTGAAACA+GGG 0.651798 8:+71552792 MsG0880046288.01.T01:CDS
GCGCGTCGAAGAAATTTCAG+AGG 0.671191 8:+71552647 MsG0880046288.01.T01:CDS
CAAGCTTGGTACAATCACTC+CGG 0.679934 8:+71552596 MsG0880046288.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAACTGTTACAAGCTCATAA+GGG - Chr8:71552775-71552794 None:intergenic 30.0%
ACACTTCTAAATCTTGGTTA+TGG - Chr8:71552562-71552581 None:intergenic 30.0%
CTTTCAACACTTCTAAATCT+TGG - Chr8:71552568-71552587 None:intergenic 30.0%
GAGACACAAAATCATCAAAT+GGG - Chr8:71552832-71552851 None:intergenic 30.0%
TTTCAAGAAGGATTGAAACA+GGG + Chr8:71552792-71552811 MsG0880046288.01.T01:CDS 30.0%
AACCAAGTCATCATCAACTA+GGG + Chr8:71552700-71552719 MsG0880046288.01.T01:CDS 35.0%
AGCTTGTAACAGTTTCAAGA+AGG + Chr8:71552780-71552799 MsG0880046288.01.T01:CDS 35.0%
GAAACTGTTACAAGCTCATA+AGG - Chr8:71552776-71552795 None:intergenic 35.0%
GGAGACACAAAATCATCAAA+TGG - Chr8:71552833-71552852 None:intergenic 35.0%
GTTTCAAGAAGGATTGAAAC+AGG + Chr8:71552791-71552810 MsG0880046288.01.T01:CDS 35.0%
TAACCAAGTCATCATCAACT+AGG + Chr8:71552699-71552718 MsG0880046288.01.T01:CDS 35.0%
TGACTTTCAACACTCACTTT+GGG + Chr8:71552748-71552767 MsG0880046288.01.T01:CDS 35.0%
ATTTCAGAGGTAAGCCTTCA+AGG + Chr8:71552660-71552679 MsG0880046288.01.T01:CDS 40.0%
GTGACTTTCAACACTCACTT+TGG + Chr8:71552747-71552766 MsG0880046288.01.T01:CDS 40.0%
GTTTCAAGCTTGAACCTTGA+AGG - Chr8:71552677-71552696 None:intergenic 40.0%
!! TTCCCTAGTTGATGATGACT+TGG - Chr8:71552705-71552724 None:intergenic 40.0%
ACTCAAAGGCTTTACACTGC+CGG - Chr8:71552618-71552637 None:intergenic 45.0%
CAAGCTTGGTACAATCACTC+CGG + Chr8:71552596-71552615 MsG0880046288.01.T01:CDS 45.0%
TGAAAGCTGTTGCACAAGCT+TGG + Chr8:71552582-71552601 MsG0880046288.01.T01:CDS 45.0%
GCGCGTCGAAGAAATTTCAG+AGG + Chr8:71552647-71552666 MsG0880046288.01.T01:CDS 50.0%
CGCGCGTCGAATTCACTCAA+AGG - Chr8:71552632-71552651 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 71552533 71552868 71552533 ID=MsG0880046288.01;Name=MsG0880046288.01
Chr8 mRNA 71552533 71552868 71552533 ID=MsG0880046288.01.T01;Parent=MsG0880046288.01;Name=MsG0880046288.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|111
Chr8 exon 71552533 71552868 71552533 ID=MsG0880046288.01.T01:exon:27847;Parent=MsG0880046288.01.T01
Chr8 CDS 71552533 71552868 71552533 ID=MsG0880046288.01.T01:cds;Parent=MsG0880046288.01.T01
Gene Sequence

>MsG0880046288.01.T01

ATGAAGAAGAAGCAACATCATGAAAACCATAACCAAGATTTAGAAGTGTTGAAAGCTGTTGCACAAGCTTGGTACAATCACTCCGGCAGTGTAAAGCCTTTGAGTGAATTCGACGCGCGTCGAAGAAATTTCAGAGGTAAGCCTTCAAGGTTCAAGCTTGAAACATTAACCAAGTCATCATCAACTAGGGAAACAAACTCTTCAACTTATTATTGTGACTTTCAACACTCACTTTGGGACCCTTATGAGCTTGTAACAGTTTCAAGAAGGATTGAAACAGGGTTAGCTTTAGATAACCCATTTGATGATTTTGTGTCTCCAAATCAGTTCAACTAA

Protein sequence

>MsG0880046288.01.T01

MKKKQHHENHNQDLEVLKAVAQAWYNHSGSVKPLSEFDARRRNFRGKPSRFKLETLTKSSSTRETNSSTYYCDFQHSLWDPYELVTVSRRIETGLALDNPFDDFVSPNQFN*