AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005800.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005800.01.T01 MTR_1g107255 69.427 157 1 2 1 110 407 563 6.27e-62 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005800.01.T01 AT5G50320 63.057 157 11 2 1 110 409 565 2.31e-56 183

Find 37 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCGTGAGCTCCATGTTTA+TGG 0.142211 1:+96272333 MsG0180005800.01.T01:CDS
CACTACTTGTCCAGAGCTTA+TGG 0.210436 1:+96272296 MsG0180005800.01.T01:CDS
ACGCCATTACTACAGAAAAT+TGG 0.214514 1:+96272749 MsG0180005800.01.T01:CDS
ACTAAGATAGCTGTAATTTC+TGG 0.226240 1:+96272717 MsG0180005800.01.T01:CDS
GGGTTATGGTACACTTCTTA+TGG 0.305200 1:+96272656 MsG0180005800.01.T01:intron
CTTGAGCAGGATATCCTTGT+TGG 0.326582 1:+96272243 MsG0180005800.01.T01:intron
ATTACGATTATTATTACATT+AGG 0.329472 1:-96272806 None:intergenic
AATTATTGTTAACAGGGTTA+TGG 0.336285 1:+96272642 MsG0180005800.01.T01:intron
GTTTAAAATGTCGCGATGTT+CGG 0.368009 1:+96271739 MsG0180005800.01.T01:CDS
ATTAGGTATTTCATCATGTA+AGG 0.374054 1:-96272789 None:intergenic
TACCTGGATTCCAGCTTCTC+TGG 0.383322 1:-96271763 None:intergenic
GGGGTTGAGAAGGGAATCTA+AGG 0.396519 1:+96271685 None:intergenic
CTAAGATAGCTGTAATTTCT+GGG 0.402023 1:+96272718 MsG0180005800.01.T01:CDS
CGCCATTACTACAGAAAATT+GGG 0.402981 1:+96272750 MsG0180005800.01.T01:CDS
ATATCCTTGTTGGTTTGTTA+CGG 0.406404 1:+96272253 MsG0180005800.01.T01:CDS
ACTACTTGTCCAGAGCTTAT+GGG 0.466121 1:+96272297 MsG0180005800.01.T01:CDS
GGTGACCTCTGGGGTTGAGA+AGG 0.496191 1:+96271675 None:intergenic
TGTTTATGGAACTGCTGTAC+CGG 0.502199 1:+96272347 MsG0180005800.01.T01:CDS
ATAGCTGTAATTTCTGGGGT+TGG 0.510369 1:+96272723 MsG0180005800.01.T01:CDS
GACCAGAGAAGCTGGAATCC+AGG 0.513139 1:+96271761 MsG0180005800.01.T01:CDS
GATGTTCGGACCAGAGAAGC+TGG 0.521187 1:+96271753 MsG0180005800.01.T01:CDS
GGAGTTAGCATTAGCTCGAA+TGG 0.524838 1:+96271707 MsG0180005800.01.T01:exon
GTGACCTCTGGGGTTGAGAA+GGG 0.529707 1:+96271676 None:intergenic
AATTGGGATATGAGCTTGAA+GGG 0.539350 1:+96272766 MsG0180005800.01.T01:CDS
TGGTACACTTCTTATGGAGG+AGG 0.541142 1:+96272662 MsG0180005800.01.T01:CDS
ATTAGCTCGAATGGAAGACT+TGG 0.543470 1:+96271716 MsG0180005800.01.T01:exon
GAACACTTACCCATAAGCTC+TGG 0.547667 1:-96272306 None:intergenic
GCTCTGGACAAGTAGTGTTA+CGG 0.560794 1:-96272290 None:intergenic
AAATTGGGATATGAGCTTGA+AGG 0.569511 1:+96272765 MsG0180005800.01.T01:CDS
TTAGCTCGAATGGAAGACTT+GGG 0.582791 1:+96271717 MsG0180005800.01.T01:exon
TTGTTACGGCTGCGAAAATG+TGG 0.617345 1:+96272267 MsG0180005800.01.T01:CDS
TTATGGTACACTTCTTATGG+AGG 0.625503 1:+96272659 MsG0180005800.01.T01:CDS
GTACAGCAGTTCCATAAACA+TGG 0.632941 1:-96272344 None:intergenic
TAAGATAGCTGTAATTTCTG+GGG 0.637498 1:+96272719 MsG0180005800.01.T01:CDS
TTCTTATGGAGGAGGCTGAG+CGG 0.672319 1:+96272670 MsG0180005800.01.T01:CDS
GGGTTGAGAAGGGAATCTAA+GGG 0.736859 1:+96271686 None:intergenic
GCAGCCGTAACAAACCAACA+AGG 0.750250 1:-96272257 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTACGATTATTATTACATT+AGG - Chr1:96272809-96272828 None:intergenic 15.0%
!!! ATCGTAATAGTTTTTTTTTA+AGG + Chr1:96272821-96272840 MsG0180005800.01.T01:three_prime_UTR 15.0%
!!! TCGTAATAGTTTTTTTTTAA+GGG + Chr1:96272822-96272841 MsG0180005800.01.T01:three_prime_UTR 15.0%
!! ATAATAATAGTTATTTGCCA+TGG + Chr1:96272850-96272869 MsG0180005800.01.T01:three_prime_UTR 20.0%
! AATAAATTTCAAGACGAGAT+TGG - Chr1:96271800-96271819 None:intergenic 25.0%
! AATATCCAAGAAGATACATA+TGG + Chr1:96272538-96272557 MsG0180005800.01.T01:intron 25.0%
! AATATCCATATGTATCTTCT+TGG - Chr1:96272546-96272565 None:intergenic 25.0%
! AATTATTGTTAACAGGGTTA+TGG + Chr1:96272642-96272661 MsG0180005800.01.T01:intron 25.0%
! AGAAAATGATGATAGACAAA+AGG - Chr1:96272052-96272071 None:intergenic 25.0%
! ATCTTCTTGGATATTATACA+TGG - Chr1:96272533-96272552 None:intergenic 25.0%
! ATTAGGTATTTCATCATGTA+AGG - Chr1:96272792-96272811 None:intergenic 25.0%
! CAACAACAAATTTACACTAA+AGG - Chr1:96272183-96272202 None:intergenic 25.0%
! GAATATTCACATGTTCTTAT+TGG + Chr1:96272449-96272468 MsG0180005800.01.T01:intron 25.0%
! GACCAAAATTATTGTTAACA+GGG + Chr1:96272636-96272655 MsG0180005800.01.T01:intron 25.0%
! TGACCAAAATTATTGTTAAC+AGG + Chr1:96272635-96272654 MsG0180005800.01.T01:intron 25.0%
!! AACCCTGTTAACAATAATTT+TGG - Chr1:96272641-96272660 None:intergenic 25.0%
!! TTTTGTCATATGAAGATACA+CGG + Chr1:96271979-96271998 MsG0180005800.01.T01:intron 25.0%
!!! CCTTCTTTATTTTGAATGAA+GGG + Chr1:96272136-96272155 MsG0180005800.01.T01:intron 25.0%
!!! CTTCTTTATTTTGAATGAAG+GGG + Chr1:96272137-96272156 MsG0180005800.01.T01:intron 25.0%
!!! TAACAATAATTTTGGTCAGT+AGG - Chr1:96272633-96272652 None:intergenic 25.0%
AAGAAATACAGAATGTCTGT+TGG - Chr1:96271877-96271896 None:intergenic 30.0%
ACTAAGATAGCTGTAATTTC+TGG + Chr1:96272717-96272736 MsG0180005800.01.T01:CDS 30.0%
ATGATGATAGACAAAAGGAT+AGG - Chr1:96272047-96272066 None:intergenic 30.0%
ATTATATGGCAAATGAAGGT+TGG + Chr1:96271950-96271969 MsG0180005800.01.T01:intron 30.0%
CCCTTCATTCAAAATAAAGA+AGG - Chr1:96272139-96272158 None:intergenic 30.0%
CTAAGATAGCTGTAATTTCT+GGG + Chr1:96272718-96272737 MsG0180005800.01.T01:CDS 30.0%
GACATTCTGTATTTCTTTGT+AGG + Chr1:96271880-96271899 MsG0180005800.01.T01:intron 30.0%
TAAGATAGCTGTAATTTCTG+GGG + Chr1:96272719-96272738 MsG0180005800.01.T01:CDS 30.0%
TACAATGAGGAACATGTTAT+TGG - Chr1:96272600-96272619 None:intergenic 30.0%
TTATATGGCAAATGAAGGTT+GGG + Chr1:96271951-96271970 MsG0180005800.01.T01:intron 30.0%
TTTACACTAAAGGTAAGAAC+AGG - Chr1:96272173-96272192 None:intergenic 30.0%
! ATCCCAATTTTCTGTAGTAA+TGG - Chr1:96272755-96272774 None:intergenic 30.0%
! TTGTTGTTGTTGATGAAACA+TGG + Chr1:96272194-96272213 MsG0180005800.01.T01:intron 30.0%
!! ATATCCTTGTTGGTTTGTTA+CGG + Chr1:96272253-96272272 MsG0180005800.01.T01:CDS 30.0%
!! ATTGGTTCTAGATAATTACC+TGG - Chr1:96271782-96271801 None:intergenic 30.0%
!!! GCCTTCTTTATTTTGAATGA+AGG + Chr1:96272135-96272154 MsG0180005800.01.T01:intron 30.0%
AAATTGGGATATGAGCTTGA+AGG + Chr1:96272765-96272784 MsG0180005800.01.T01:CDS 35.0%
AATTGGGATATGAGCTTGAA+GGG + Chr1:96272766-96272785 MsG0180005800.01.T01:CDS 35.0%
ACGCCATTACTACAGAAAAT+TGG + Chr1:96272749-96272768 MsG0180005800.01.T01:CDS 35.0%
ACTTGTTCGTCGTGATTATA+TGG + Chr1:96271936-96271955 MsG0180005800.01.T01:intron 35.0%
CGCCATTACTACAGAAAATT+GGG + Chr1:96272750-96272769 MsG0180005800.01.T01:CDS 35.0%
CGTGATTATATGGCAAATGA+AGG + Chr1:96271946-96271965 MsG0180005800.01.T01:intron 35.0%
GAACATGTTATTGGTATCCT+TGG - Chr1:96272591-96272610 None:intergenic 35.0%
TTATGGTACACTTCTTATGG+AGG + Chr1:96272659-96272678 MsG0180005800.01.T01:CDS 35.0%
! ATTAGCATGACAGTTTTACC+TGG - Chr1:96272403-96272422 None:intergenic 35.0%
! GTTTAAAATGTCGCGATGTT+CGG + Chr1:96271739-96271758 MsG0180005800.01.T01:CDS 35.0%
! TGAGTGTAATTGCTGAGTTT+TGG + Chr1:96272480-96272499 MsG0180005800.01.T01:intron 35.0%
!! ATTTTGGTCAGTAGGTCAAT+TGG - Chr1:96272625-96272644 None:intergenic 35.0%
ACTACTTGTCCAGAGCTTAT+GGG + Chr1:96272297-96272316 MsG0180005800.01.T01:CDS 40.0%
AGGATAGGCACTAATAAGAG+AGG - Chr1:96272032-96272051 None:intergenic 40.0%
AGGTCAATTGGAGTACAATG+AGG - Chr1:96272613-96272632 None:intergenic 40.0%
ATCACCAGATTAACCAGAAG+AGG + Chr1:96271909-96271928 MsG0180005800.01.T01:intron 40.0%
ATGAAACATGGCCATGACAT+TGG + Chr1:96272206-96272225 MsG0180005800.01.T01:intron 40.0%
ATTAGCTCGAATGGAAGACT+TGG + Chr1:96271716-96271735 MsG0180005800.01.T01:exon 40.0%
GAAGGCAAAATATGCACAAC+AGG - Chr1:96272121-96272140 None:intergenic 40.0%
GGGTTATGGTACACTTCTTA+TGG + Chr1:96272656-96272675 MsG0180005800.01.T01:intron 40.0%
GTACAGCAGTTCCATAAACA+TGG - Chr1:96272347-96272366 None:intergenic 40.0%
TGTTTATGGAACTGCTGTAC+CGG + Chr1:96272347-96272366 MsG0180005800.01.T01:CDS 40.0%
TTAGCTCGAATGGAAGACTT+GGG + Chr1:96271717-96271736 MsG0180005800.01.T01:exon 40.0%
! ATCACAATGCAGCTTTTCCA+AGG + Chr1:96272571-96272590 MsG0180005800.01.T01:intron 40.0%
!! ATAGCTGTAATTTCTGGGGT+TGG + Chr1:96272723-96272742 MsG0180005800.01.T01:CDS 40.0%
ACCAGATTAACCAGAAGAGG+TGG + Chr1:96271912-96271931 MsG0180005800.01.T01:intron 45.0%
AGACAGCACAACCAATGTCA+TGG - Chr1:96272220-96272239 None:intergenic 45.0%
CACTACTTGTCCAGAGCTTA+TGG + Chr1:96272296-96272315 MsG0180005800.01.T01:CDS 45.0%
CTTGAGCAGGATATCCTTGT+TGG + Chr1:96272243-96272262 MsG0180005800.01.T01:intron 45.0%
GAACACTTACCCATAAGCTC+TGG - Chr1:96272309-96272328 None:intergenic 45.0%
GGAGTTAGCATTAGCTCGAA+TGG + Chr1:96271707-96271726 MsG0180005800.01.T01:exon 45.0%
GTCATATGAAGATACACGGC+AGG + Chr1:96271983-96272002 MsG0180005800.01.T01:intron 45.0%
GTTCGTGAGCTCCATGTTTA+TGG + Chr1:96272333-96272352 MsG0180005800.01.T01:CDS 45.0%
TAAGAGAGGCTGAGGAAAGA+AGG - Chr1:96272018-96272037 None:intergenic 45.0%
TGGTACACTTCTTATGGAGG+AGG + Chr1:96272662-96272681 MsG0180005800.01.T01:CDS 45.0%
TTCTGACAAGCTACAACACC+AGG + Chr1:96272382-96272401 MsG0180005800.01.T01:intron 45.0%
TTGTTACGGCTGCGAAAATG+TGG + Chr1:96272267-96272286 MsG0180005800.01.T01:CDS 45.0%
! GCTCTGGACAAGTAGTGTTA+CGG - Chr1:96272293-96272312 None:intergenic 45.0%
! GTGTGAGTCTAGTTTGTAGC+TGG + Chr1:96272087-96272106 MsG0180005800.01.T01:intron 45.0%
! TCCACCTCTTCTGGTTAATC+TGG - Chr1:96271916-96271935 None:intergenic 45.0%
CGAACAAGTTCCACCTCTTC+TGG - Chr1:96271925-96271944 None:intergenic 50.0%
GCAGCCGTAACAAACCAACA+AGG - Chr1:96272260-96272279 None:intergenic 50.0%
GGCACTAATAAGAGAGGCTG+AGG - Chr1:96272026-96272045 None:intergenic 50.0%
TACCTGGATTCCAGCTTCTC+TGG - Chr1:96271766-96271785 None:intergenic 50.0%
TGTGCTGTCTTCTCTTGAGC+AGG + Chr1:96272230-96272249 MsG0180005800.01.T01:intron 50.0%
TTCTTATGGAGGAGGCTGAG+CGG + Chr1:96272670-96272689 MsG0180005800.01.T01:CDS 50.0%
! GTCTAGTTTGTAGCTGGCAC+TGG + Chr1:96272093-96272112 MsG0180005800.01.T01:intron 50.0%
!! GAACTGCTGTACCGGTTCAT+GGG + Chr1:96272355-96272374 MsG0180005800.01.T01:intron 50.0%
GACCAGAGAAGCTGGAATCC+AGG + Chr1:96271761-96271780 MsG0180005800.01.T01:CDS 55.0%
GATGTTCGGACCAGAGAAGC+TGG + Chr1:96271753-96271772 MsG0180005800.01.T01:CDS 55.0%
GTCAGAATCCGCCCATGAAC+CGG - Chr1:96272369-96272388 None:intergenic 55.0%
!! GGAACTGCTGTACCGGTTCA+TGG + Chr1:96272354-96272373 MsG0180005800.01.T01:intron 55.0%
!! CTGCTGTACCGGTTCATGGG+CGG + Chr1:96272358-96272377 MsG0180005800.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 96271687 96272870 96271687 ID=MsG0180005800.01;Name=MsG0180005800.01
Chr1 mRNA 96271687 96272870 96271687 ID=MsG0180005800.01.T01;Parent=MsG0180005800.01;Name=MsG0180005800.01.T01;_AED=0.48;_eAED=0.48;_QI=39|0|0.66|1|1|1|3|55|110
Chr1 exon 96271687 96271782 96271687 ID=MsG0180005800.01.T01:exon:50481;Parent=MsG0180005800.01.T01
Chr1 exon 96272252 96272368 96272252 ID=MsG0180005800.01.T01:exon:50482;Parent=MsG0180005800.01.T01
Chr1 exon 96272657 96272870 96272657 ID=MsG0180005800.01.T01:exon:50483;Parent=MsG0180005800.01.T01
Chr1 five_prime_UTR 96271687 96271725 96271687 ID=MsG0180005800.01.T01:five_prime_utr;Parent=MsG0180005800.01.T01
Chr1 CDS 96271726 96271782 96271726 ID=MsG0180005800.01.T01:cds;Parent=MsG0180005800.01.T01
Chr1 CDS 96272252 96272368 96272252 ID=MsG0180005800.01.T01:cds;Parent=MsG0180005800.01.T01
Chr1 CDS 96272657 96272815 96272657 ID=MsG0180005800.01.T01:cds;Parent=MsG0180005800.01.T01
Chr1 three_prime_UTR 96272816 96272870 96272816 ID=MsG0180005800.01.T01:three_prime_utr;Parent=MsG0180005800.01.T01
Gene Sequence

>MsG0180005800.01.T01

ATGGAAGACTTGGGTTTAAAATGTCGCGATGTTCGGACCAGAGAAGCTGGAATCCAGGATATCCTTGTTGGTTTGTTACGGCTGCGAAAATGTGGCCGTAACACTACTTGTCCAGAGCTTATGGGTAAGTGTTCTATAGTTCGTGAGCTCCATGTTTATGGAACTGCTGTACCGGGTTATGGTACACTTCTTATGGAGGAGGCTGAGCGGATTGCTTCGAAAGAACACAGATCAACTAAGATAGCTGTAATTTCTGGGGTTGGCACACGCCATTACTACAGAAAATTGGGATATGAGCTTGAAGGGCCTTACATGATGAAATACCTAATGTAA

Protein sequence

>MsG0180005800.01.T01

MEDLGLKCRDVRTREAGIQDILVGLLRLRKCGRNTTCPELMGKCSIVRELHVYGTAVPGYGTLLMEEAERIASKEHRSTKIAVISGVGTRHYYRKLGYELEGPYMMKYLM*