AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007875.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007875.01.T01 MTR_2g034640 98.876 89 1 0 1 89 1 89 9.14e-61 188
MsG0280007875.01.T01 MTR_4g121860 93.258 89 6 0 1 89 1 89 1.10e-58 183
MsG0280007875.01.T01 MTR_7g112090 93.258 89 6 0 1 89 1 89 9.34e-58 181
MsG0280007875.01.T01 MTR_5g098820 71.698 53 14 1 39 90 1 53 2.81e-19 80.5
MsG0280007875.01.T01 MTR_5g098820 68.750 48 14 1 44 90 69 116 2.43e-15 70.1
MsG0280007875.01.T01 MTR_4g128910 43.056 72 41 0 18 89 15 86 7.08e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007875.01.T01 AT1G56330 97.753 89 2 0 1 89 1 89 2.82e-60 187
MsG0280007875.01.T01 AT4G02080 95.506 89 4 0 1 89 1 89 2.49e-59 185
MsG0280007875.01.T01 AT3G62560 94.382 89 5 0 1 89 1 89 1.04e-58 183
MsG0280007875.01.T01 AT1G09180 93.258 89 6 0 1 89 1 89 1.67e-55 175
MsG0280007875.01.T01 AT2G24765 43.056 72 41 0 18 89 15 86 8.98e-12 60.8
MsG0280007875.01.T01 AT2G24765 43.056 72 41 0 18 89 15 86 6.25e-11 59.7

Find 42 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAAACCAACCCATTCTTT+TGG 0.265477 2:+22174061 MsG0280007875.01.T01:five_prime_UTR
TTCATCTTCGATTGGTTCTA+TGG 0.267772 2:+22174108 MsG0280007875.01.T01:CDS
CTCTGTCTGTTGTGTTGTCT+TGG 0.283623 2:-22173943 None:intergenic
ATCAAATTGCTCGTAGAGTT+TGG 0.294302 2:+22174412 MsG0280007875.01.T01:CDS
AGTGTTGTTGTCCGTGATTT+TGG 0.325020 2:+22174989 MsG0280007875.01.T01:CDS
CTTCGGAGGAATTGAGTATT+GGG 0.328437 2:+22174355 MsG0280007875.01.T01:CDS
TGTCTGTATCCGTGTTTCAT+AGG 0.344036 2:+22174768 MsG0280007875.01.T01:CDS
ACACTTCCATTCTCATGAAT+TGG 0.348179 2:+22174954 MsG0280007875.01.T01:CDS
TTCTTGCATCCCTTGGTCTC+TGG 0.356031 2:+22174133 MsG0280007875.01.T01:CDS
TATGGCATTCTTGCATCCCT+TGG 0.385266 2:+22174126 MsG0280007875.01.T01:CDS
CAACCATGTTCATCTTCGAT+TGG 0.422638 2:+22174100 MsG0280007875.01.T01:exon
GAAGCCAAGATTCTCTTCCT+TGG 0.425299 2:+22174162 MsG0280007875.01.T01:CDS
GAGATCTAAACCCAAAAGAA+TGG 0.439163 2:-22174072 None:intergenic
TTCCTTGGTCTTGATAATGC+TGG 0.455864 2:+22174177 MsG0280007875.01.T01:CDS
ACTTCGGAGGAATTGAGTAT+TGG 0.465946 2:+22174354 MsG0280007875.01.T01:CDS
CATCTTTGAGCATATGAAGA+AGG 0.466211 2:-22174209 None:intergenic
TTCCTCCGAAGTAGGATGTT+GGG 0.479835 2:-22174343 None:intergenic
TTGGAAAGATTATTATGCTA+AGG 0.487117 2:+22174431 MsG0280007875.01.T01:CDS
AAGACCAAGGAAGAGAATCT+TGG 0.490940 2:-22174166 None:intergenic
AGATCTAAACCCAAAAGAAT+GGG 0.505942 2:-22174071 None:intergenic
TTGACACCAATTCATGAGAA+TGG 0.506722 2:-22174960 None:intergenic
CATGACATGTGTCGGACACA+TGG 0.520070 2:-22174658 None:intergenic
ATACTCAATTCCTCCGAAGT+AGG 0.542870 2:-22174351 None:intergenic
ATTCCTCCGAAGTAGGATGT+TGG 0.553310 2:-22174344 None:intergenic
TTCAGTGTAATTATAAGTGT+CGG 0.569208 2:-22174705 None:intergenic
CTAAACCCAAAAGAATGGGT+TGG 0.570954 2:-22174067 None:intergenic
TCTTCATATGCTCAAAGATG+AGG 0.580955 2:+22174212 MsG0280007875.01.T01:CDS
ATCTTTGAGCATATGAAGAA+GGG 0.582029 2:-22174208 None:intergenic
CACATGGACATGAGGAGTGT+CGG 0.589512 2:-22174642 None:intergenic
CATAATCATAACCAAAATCA+CGG 0.598688 2:-22175000 None:intergenic
ACCGACCCAACATCCTACTT+CGG 0.614242 2:+22174338 MsG0280007875.01.T01:CDS
CACATGTCATGTTGGATGTG+TGG 0.614696 2:+22174670 MsG0280007875.01.T01:CDS
TTCCAGCATTATCAAGACCA+AGG 0.615002 2:-22174179 None:intergenic
ACATCCAACATGACATGTGT+CGG 0.616393 2:-22174666 None:intergenic
TCCGAAGTAGGATGTTGGGT+CGG 0.619899 2:-22174339 None:intergenic
GTGTCGGACACATGGACATG+AGG 0.632962 2:-22174650 None:intergenic
GTAATTATAAGTGTCGGTGT+CGG 0.633351 2:-22174699 None:intergenic
GTAACTACATGTGTAAGTGT+CGG 0.641611 2:-22174926 None:intergenic
GAACCAATCGAAGATGAACA+TGG 0.652038 2:-22174103 None:intergenic
GTGTCCGACACATGTCATGT+TGG 0.659396 2:+22174662 MsG0280007875.01.T01:CDS
GACCCAACATCCTACTTCGG+AGG 0.659761 2:+22174341 MsG0280007875.01.T01:CDS
AAATCAGCACCTATGAAACA+CGG 0.686966 2:-22174777 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTGGTTTTTAATTTA+GGG + Chr2:22174484-22174503 MsG0280007875.01.T01:CDS 10.0%
!! ATAAATATAATGATCTATCT+AGG + Chr2:22174891-22174910 MsG0280007875.01.T01:intron 15.0%
!!! ATTTGTTTGTTATCAATTTT+TGG + Chr2:22174470-22174489 MsG0280007875.01.T01:CDS 15.0%
!!! CAATTTTTGGTTTTTAATTT+AGG + Chr2:22174483-22174502 MsG0280007875.01.T01:CDS 15.0%
!!! AAATCGAATTTTGAATCAAA+AGG - Chr2:22174262-22174281 None:intergenic 20.0%
!!! AAGTTTAAAGCTTTTGATTT+GGG + Chr2:22174384-22174403 MsG0280007875.01.T01:CDS 20.0%
!!! AATCGAATTTTGAATCAAAA+GGG - Chr2:22174261-22174280 None:intergenic 20.0%
!!! TGATTTTCTCAAATTATTAG+TGG + Chr2:22174733-22174752 MsG0280007875.01.T01:CDS 20.0%
!!! TTCATATTTTTAGACTCTAT+TGG + Chr2:22174857-22174876 MsG0280007875.01.T01:intron 20.0%
!!! TTTTTAATTTAGGGATTAGT+TGG + Chr2:22174493-22174512 MsG0280007875.01.T01:CDS 20.0%
! ATAAAAATGTTACAAAGACC+AGG - Chr2:22174834-22174853 None:intergenic 25.0%
! CATAATCATAACCAAAATCA+CGG - Chr2:22175003-22175022 None:intergenic 25.0%
! TTCAGTGTAATTATAAGTGT+CGG - Chr2:22174708-22174727 None:intergenic 25.0%
! TTGGAAAGATTATTATGCTA+AGG + Chr2:22174431-22174450 MsG0280007875.01.T01:CDS 25.0%
!!! CAAGTTTAAAGCTTTTGATT+TGG + Chr2:22174383-22174402 MsG0280007875.01.T01:CDS 25.0%
AGATCTAAACCCAAAAGAAT+GGG - Chr2:22174074-22174093 None:intergenic 30.0%
ATCTTTGAGCATATGAAGAA+GGG - Chr2:22174211-22174230 None:intergenic 30.0%
TACTACGATGTTCAAAAACT+TGG + Chr2:22175030-22175049 MsG0280007875.01.T01:CDS 30.0%
TCAAAAGGGTCAAAGAAAAA+AGG - Chr2:22174247-22174266 None:intergenic 30.0%
! AATTTGTTTCAGCTATTGCA+TGG + Chr2:22174567-22174586 MsG0280007875.01.T01:intron 30.0%
! GTTTTGTGAATCTGTGAAAT+GGG - Chr2:22174048-22174067 None:intergenic 30.0%
!!! TTTAAAGCTTTTGATTTGGG+TGG + Chr2:22174387-22174406 MsG0280007875.01.T01:CDS 30.0%
AAATCAGCACCTATGAAACA+CGG - Chr2:22174780-22174799 None:intergenic 35.0%
ACACTTCCATTCTCATGAAT+TGG + Chr2:22174954-22174973 MsG0280007875.01.T01:CDS 35.0%
ATCAAATTGCTCGTAGAGTT+TGG + Chr2:22174412-22174431 MsG0280007875.01.T01:CDS 35.0%
CATCTTTGAGCATATGAAGA+AGG - Chr2:22174212-22174231 None:intergenic 35.0%
GAGATCTAAACCCAAAAGAA+TGG - Chr2:22174075-22174094 None:intergenic 35.0%
GTAACTACATGTGTAAGTGT+CGG - Chr2:22174929-22174948 None:intergenic 35.0%
GTTTGTGAAAGTCTGTTGAA+AGG + Chr2:22174537-22174556 MsG0280007875.01.T01:intron 35.0%
TCTTCATATGCTCAAAGATG+AGG + Chr2:22174212-22174231 MsG0280007875.01.T01:CDS 35.0%
TGAAAGTCTGTTGAAAGGTT+TGG + Chr2:22174542-22174561 MsG0280007875.01.T01:intron 35.0%
TTGACACCAATTCATGAGAA+TGG - Chr2:22174963-22174982 None:intergenic 35.0%
! ACAAAACCAACCCATTCTTT+TGG + Chr2:22174061-22174080 MsG0280007875.01.T01:five_prime_UTR 35.0%
! CAAAACCAACCCATTCTTTT+GGG + Chr2:22174062-22174081 MsG0280007875.01.T01:five_prime_UTR 35.0%
!! GGTTTTGTGAATCTGTGAAA+TGG - Chr2:22174049-22174068 None:intergenic 35.0%
!! GTAATTATAAGTGTCGGTGT+CGG - Chr2:22174702-22174721 None:intergenic 35.0%
!! TTCATCTTCGATTGGTTCTA+TGG + Chr2:22174108-22174127 MsG0280007875.01.T01:CDS 35.0%
!!! CTGATTTGTTTTCAGTTGGA+TGG + Chr2:22174793-22174812 MsG0280007875.01.T01:intron 35.0%
AAGACCAAGGAAGAGAATCT+TGG - Chr2:22174169-22174188 None:intergenic 40.0%
ACATCCAACATGACATGTGT+CGG - Chr2:22174669-22174688 None:intergenic 40.0%
ACTTCGGAGGAATTGAGTAT+TGG + Chr2:22174354-22174373 MsG0280007875.01.T01:CDS 40.0%
ATACTCAATTCCTCCGAAGT+AGG - Chr2:22174354-22174373 None:intergenic 40.0%
CAACCATGTTCATCTTCGAT+TGG + Chr2:22174100-22174119 MsG0280007875.01.T01:exon 40.0%
CTAAACCCAAAAGAATGGGT+TGG - Chr2:22174070-22174089 None:intergenic 40.0%
CTTCGGAGGAATTGAGTATT+GGG + Chr2:22174355-22174374 MsG0280007875.01.T01:CDS 40.0%
GAACCAATCGAAGATGAACA+TGG - Chr2:22174106-22174125 None:intergenic 40.0%
TGTCTGTATCCGTGTTTCAT+AGG + Chr2:22174768-22174787 MsG0280007875.01.T01:CDS 40.0%
TTCCAGCATTATCAAGACCA+AGG - Chr2:22174182-22174201 None:intergenic 40.0%
! TTCTTTTTGCCAGAGACCAA+GGG - Chr2:22174145-22174164 None:intergenic 40.0%
!! AGTGTTGTTGTCCGTGATTT+TGG + Chr2:22174989-22175008 MsG0280007875.01.T01:CDS 40.0%
!! TTCCTTGGTCTTGATAATGC+TGG + Chr2:22174177-22174196 MsG0280007875.01.T01:CDS 40.0%
!!! GGTGCTGATTTGTTTTCAGT+TGG + Chr2:22174789-22174808 MsG0280007875.01.T01:intron 40.0%
AGTTGGATGGATCTGTTGAC+AGG + Chr2:22174806-22174825 MsG0280007875.01.T01:intron 45.0%
ATTCCTCCGAAGTAGGATGT+TGG - Chr2:22174347-22174366 None:intergenic 45.0%
CACATGTCATGTTGGATGTG+TGG + Chr2:22174670-22174689 MsG0280007875.01.T01:CDS 45.0%
CTCTGTCTGTTGTGTTGTCT+TGG - Chr2:22173946-22173965 None:intergenic 45.0%
GAAGCCAAGATTCTCTTCCT+TGG + Chr2:22174162-22174181 MsG0280007875.01.T01:CDS 45.0%
TATGGCATTCTTGCATCCCT+TGG + Chr2:22174126-22174145 MsG0280007875.01.T01:CDS 45.0%
TTCCTCCGAAGTAGGATGTT+GGG - Chr2:22174346-22174365 None:intergenic 45.0%
! CTTCTTTTTGCCAGAGACCA+AGG - Chr2:22174146-22174165 None:intergenic 45.0%
ACCGACCCAACATCCTACTT+CGG + Chr2:22174338-22174357 MsG0280007875.01.T01:CDS 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - Chr2:22173983-22174002 None:intergenic 50.0%
CATGACATGTGTCGGACACA+TGG - Chr2:22174661-22174680 None:intergenic 50.0%
CTCTCTCTCTCTCTCTCTGT+AGG + Chr2:22173997-22174016 MsG0280007875.01.T01:five_prime_UTR 50.0%
GTGTCCGACACATGTCATGT+TGG + Chr2:22174662-22174681 MsG0280007875.01.T01:CDS 50.0%
TCCGAAGTAGGATGTTGGGT+CGG - Chr2:22174342-22174361 None:intergenic 50.0%
TGGATCTGTTGACAGGTTCC+TGG + Chr2:22174813-22174832 MsG0280007875.01.T01:intron 50.0%
TTCTTGCATCCCTTGGTCTC+TGG + Chr2:22174133-22174152 MsG0280007875.01.T01:CDS 50.0%
! CACATGGACATGAGGAGTGT+CGG - Chr2:22174645-22174664 None:intergenic 50.0%
GACCCAACATCCTACTTCGG+AGG + Chr2:22174341-22174360 MsG0280007875.01.T01:CDS 55.0%
GTGTCGGACACATGGACATG+AGG - Chr2:22174653-22174672 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 22173930 22175063 22173930 ID=MsG0280007875.01;Name=MsG0280007875.01
Chr2 mRNA 22173930 22175063 22173930 ID=MsG0280007875.01.T01;Parent=MsG0280007875.01;Name=MsG0280007875.01.T01;_AED=0.37;_eAED=0.65;_QI=175|0.33|0.25|1|1|1|4|0|211
Chr2 exon 22173930 22174233 22173930 ID=MsG0280007875.01.T01:exon:2879;Parent=MsG0280007875.01.T01
Chr2 exon 22174321 22174526 22174321 ID=MsG0280007875.01.T01:exon:2880;Parent=MsG0280007875.01.T01
Chr2 exon 22174640 22174789 22174640 ID=MsG0280007875.01.T01:exon:2881;Parent=MsG0280007875.01.T01
Chr2 exon 22174913 22175063 22174913 ID=MsG0280007875.01.T01:exon:2882;Parent=MsG0280007875.01.T01
Chr2 five_prime_UTR 22173930 22174104 22173930 ID=MsG0280007875.01.T01:five_prime_utr;Parent=MsG0280007875.01.T01
Chr2 CDS 22174105 22174233 22174105 ID=MsG0280007875.01.T01:cds;Parent=MsG0280007875.01.T01
Chr2 CDS 22174321 22174526 22174321 ID=MsG0280007875.01.T01:cds;Parent=MsG0280007875.01.T01
Chr2 CDS 22174640 22174789 22174640 ID=MsG0280007875.01.T01:cds;Parent=MsG0280007875.01.T01
Chr2 CDS 22174913 22175063 22174913 ID=MsG0280007875.01.T01:cds;Parent=MsG0280007875.01.T01
Gene Sequence

>MsG0280007875.01.T01

ATGTTCATCTTCGATTGGTTCTATGGCATTCTTGCATCCCTTGGTCTCTGGCAAAAAGAAGCCAAGATTCTCTTCCTTGGTCTTGATAATGCTGGAAAAACTACCCTTCTTCATATGCTCAAAGATGAGAGATTAGTTCAACATCAACCGACCCAACATCCTACTTCGGAGGAATTGAGTATTGGGAAGATCAAGTTTAAAGCTTTTGATTTGGGTGGTCATCAAATTGCTCGTAGAGTTTGGAAAGATTATTATGCTAAGGTTGAATTTCTTGATCTATTTGTTTGTTATCAATTTTTGGTTTTTAATTTAGGGATTAGTTGGAATTTTTTTATCACCGACACTCCTCATGTCCATGTGTCCGACACATGTCATGTTGGATGTGTGGATGTGTCCGACACCGACACTTATAATTACACTGAATTATGTGATTTTCTCAAATTATTAGTGGTGTTTGTGTTCGTGTCTGTATCCGTGTTTCATAGGCGTGTCACGTGTCCGACACTTACACATGTAGTTACATTTAACACTTCCATTCTCATGAATTGGTGTCAACGTGTCAGTGTTGTTGTCCGTGATTTTGGTTATGATTATGATGCATCTACTACGATGTTCAAAAACTTGGCTATTCTTTAG

Protein sequence

>MsG0280007875.01.T01

MFIFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVEFLDLFVCYQFLVFNLGISWNFFITDTPHVHVSDTCHVGCVDVSDTDTYNYTELCDFLKLLVVFVFVSVSVFHRRVTCPTLTHVVTFNTSILMNWCQRVSVVVRDFGYDYDASTTMFKNLAIL*