AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007908.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007908.01.T01 MTR_2g035100 99.363 157 1 0 1 157 1 157 2.90e-109 308
MsG0280007908.01.T01 MTR_2g035105 98.089 157 3 0 1 157 1 157 6.47e-108 304
MsG0280007908.01.T01 MTR_2g035130 88.535 157 18 0 1 157 1 157 3.93e-97 277
MsG0280007908.01.T01 MTR_2g035150 84.713 157 24 0 1 157 1 157 2.25e-93 268
MsG0280007908.01.T01 MTR_2g035120 83.439 157 26 0 1 157 1 157 3.53e-93 267
MsG0280007908.01.T01 MTR_6g033450 71.975 157 44 0 1 157 1 157 2.13e-79 232
MsG0280007908.01.T01 MTR_4g120970 71.975 157 44 0 1 157 1 157 2.13e-79 232
MsG0280007908.01.T01 MTR_2g035320 65.605 157 54 0 1 157 1 157 2.81e-69 206
MsG0280007908.01.T01 MTR_2g035190 61.783 157 60 0 1 157 1 157 6.19e-66 198
MsG0280007908.01.T01 MTR_2g035210 61.146 157 61 0 1 157 1 157 9.87e-65 195
MsG0280007908.01.T01 MTR_2g035220 61.783 157 60 0 1 157 1 157 9.72e-64 192
MsG0280007908.01.T01 MTR_2g035170 59.873 157 63 0 1 157 1 157 4.58e-62 188
MsG0280007908.01.T01 MTR_2g435310 40.385 156 92 1 1 155 1 156 7.90e-39 129
MsG0280007908.01.T01 MTR_2g035320 63.542 96 35 0 62 157 27 122 5.07e-37 124
MsG0280007908.01.T01 MTR_4g120760 39.744 156 93 1 1 155 1 156 1.94e-36 123
MsG0280007908.01.T01 MTR_4g120760 39.744 156 93 1 1 155 1 156 2.21e-36 123
MsG0280007908.01.T01 MTR_4g120950 44.720 161 82 5 1 157 1 158 2.65e-33 115
MsG0280007908.01.T01 MTR_4g120940 62.500 88 30 1 70 157 35 119 2.50e-30 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGAAAGTAAGTTTCTTGA+TGG 0.287513 2:-22782903 None:intergenic
AGCTATATGCTTTAGTAATC+AGG 0.289844 2:-22784255 None:intergenic
CCCTTGATTTCCTCTTCACT+TGG 0.329042 2:-22784174 None:intergenic
CTTCAACAATTTCAATACTT+TGG 0.342783 2:-22782862 None:intergenic
ATCAATAACCTTTGGGATAA+GGG 0.353978 2:-22782834 None:intergenic
GGATGGCATCAATAACCTTT+GGG 0.355456 2:-22782841 None:intergenic
ACTTGGTGCAGCATCACCTT+TGG 0.357443 2:-22784157 None:intergenic
GAGATCTTCTCCACTGTGTC+TGG 0.364866 2:-22784066 None:intergenic
TGGATGGCATCAATAACCTT+TGG 0.369451 2:-22782842 None:intergenic
AAGAAACTTACTTTCGTTGA+AGG 0.370871 2:+22782908 MsG0280007908.01.T01:CDS
GGCTCTTGAAGGTTACGTTT+TGG 0.372797 2:+22784227 MsG0280007908.01.T01:CDS
CATCAATAACCTTTGGGATA+AGG 0.374566 2:-22782835 None:intergenic
AGTAAGTTTCTTGATGGTTC+CGG 0.383147 2:-22782897 None:intergenic
CAAAGTATTGAAATTGTTGA+AGG 0.421501 2:+22782863 MsG0280007908.01.T01:CDS
TATATAATTCATTATCATCA+TGG 0.424718 2:+22782730 MsG0280007908.01.T01:exon
GCTTACAACTACAGCATAGT+TGG 0.432371 2:+22784030 MsG0280007908.01.T01:CDS
CATGGGTGTCTTCAACTTTG+AGG 0.436771 2:+22782748 MsG0280007908.01.T01:exon
AACAATTTCAATACTTTGGA+TGG 0.439896 2:-22782858 None:intergenic
ATTGAAATTGTTGAAGGAAA+CGG 0.445615 2:+22782869 MsG0280007908.01.T01:CDS
TTTGAGTCTAAATTGTCTGC+AGG 0.453113 2:+22784090 MsG0280007908.01.T01:CDS
TTTGCAATGGATCCTCCATC+TGG 0.453770 2:-22784114 None:intergenic
TACAGCATAGTTGGTGGTGG+TGG 0.475041 2:+22784039 MsG0280007908.01.T01:CDS
AGAGCTTTGTAAAGTCTAGC+AGG 0.480944 2:-22782794 None:intergenic
ACCAAGTGAAGAGGAAATCA+AGG 0.498836 2:+22784173 MsG0280007908.01.T01:CDS
TTGCTTGCTTGCTTTATACA+TGG 0.499671 2:+22784280 MsG0280007908.01.T01:three_prime_UTR
ATATAATTCATTATCATCAT+GGG 0.524327 2:+22782731 MsG0280007908.01.T01:exon
AAAGTATGATTTGCACAAAG+TGG 0.527363 2:+22783984 MsG0280007908.01.T01:CDS
TTTCACAGTAAGCTTTGCAA+TGG 0.527944 2:-22784127 None:intergenic
GTTGAAGGAAACGGTGGCGC+CGG 0.542615 2:+22782878 MsG0280007908.01.T01:CDS
TGCAGACAATTTAGACTCAA+AGG 0.553148 2:-22784088 None:intergenic
AAATTGTCTGCAGGGCCAGA+TGG 0.558648 2:+22784099 MsG0280007908.01.T01:CDS
GGTGGCAAAGCTAGAGGTGA+TGG 0.567538 2:+22784195 MsG0280007908.01.T01:CDS
TTCTGATACCCTTATCCCAA+AGG 0.569991 2:+22782826 MsG0280007908.01.T01:CDS
TTGAGTCTAAATTGTCTGCA+GGG 0.579973 2:+22784091 MsG0280007908.01.T01:CDS
TGATGCTGCACCAAGTGAAG+AGG 0.603594 2:+22784164 MsG0280007908.01.T01:CDS
TTGTCTGCAGGGCCAGATGG+AGG 0.618761 2:+22784102 MsG0280007908.01.T01:CDS
TACAACTACAGCATAGTTGG+TGG 0.619903 2:+22784033 MsG0280007908.01.T01:CDS
AACTACAGCATAGTTGGTGG+TGG 0.623337 2:+22784036 MsG0280007908.01.T01:CDS
ATCAAGGGTGGCAAAGCTAG+AGG 0.623874 2:+22784189 MsG0280007908.01.T01:CDS
AGCAGGAGCTACAATAGATG+TGG 0.635518 2:-22782777 None:intergenic
ACTGTGAAATACTTCACCAA+AGG 0.653868 2:+22784141 MsG0280007908.01.T01:CDS
CCAAGTGAAGAGGAAATCAA+GGG 0.664848 2:+22784174 MsG0280007908.01.T01:CDS
GAAATTGTTGAAGGAAACGG+TGG 0.666040 2:+22782872 MsG0280007908.01.T01:CDS
AGTGAAGAGGAAATCAAGGG+TGG 0.695634 2:+22784177 MsG0280007908.01.T01:CDS
TGGTGGACTTCCAGACACAG+TGG 0.739823 2:+22784056 MsG0280007908.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAACATTTTATTATACATA+GGG - Chr2:22782986-22783005 None:intergenic 10.0%
!!! TTAAACATTTTATTATACAT+AGG - Chr2:22782987-22783006 None:intergenic 10.0%
!! ATATAATTCATTATCATCAT+GGG + Chr2:22782731-22782750 MsG0280007908.01.T01:exon 15.0%
!! TATATAATTCATTATCATCA+TGG + Chr2:22782730-22782749 MsG0280007908.01.T01:exon 15.0%
!!! ATATTGGATTTTTTTTTTGA+AGG + Chr2:22783698-22783717 MsG0280007908.01.T01:intron 15.0%
!!! TTTTTGAAGGAATTATATAT+TGG + Chr2:22783711-22783730 MsG0280007908.01.T01:intron 15.0%
!! AAACTACCAATATAAGTATT+TGG - Chr2:22783320-22783339 None:intergenic 20.0%
!! AAGATACATAACATAAATAG+TGG + Chr2:22783386-22783405 MsG0280007908.01.T01:intron 20.0%
!! CTACATTGAAATTTCATAAT+TGG - Chr2:22783172-22783191 None:intergenic 20.0%
!! TAATATGTGAAAAATTGAAG+TGG + Chr2:22782943-22782962 MsG0280007908.01.T01:intron 20.0%
!!! ACAGTTTTTTTTTTTTGGTT+AGG - Chr2:22783577-22783596 None:intergenic 20.0%
!!! AGTTTTTAAATAGCCTTATT+TGG - Chr2:22783545-22783564 None:intergenic 20.0%
!!! GAGAAACAGTTTTTTTTTTT+TGG - Chr2:22783582-22783601 None:intergenic 20.0%
!!! TTTTTACCGTAAAACAATAT+GGG + Chr2:22783345-22783364 MsG0280007908.01.T01:intron 20.0%
!!! TTTTTTACCGTAAAACAATA+TGG + Chr2:22783344-22783363 MsG0280007908.01.T01:intron 20.0%
! AACAAGAATCAACAATTTGA+TGG - Chr2:22783643-22783662 None:intergenic 25.0%
! AACAATTTCAATACTTTGGA+TGG - Chr2:22782861-22782880 None:intergenic 25.0%
! ATTCACCATAATACCAAATA+AGG + Chr2:22783529-22783548 MsG0280007908.01.T01:intron 25.0%
! ATTGAAATTGTTGAAGGAAA+CGG + Chr2:22782869-22782888 MsG0280007908.01.T01:CDS 25.0%
! CATTGAAATTTCATAATTGG+AGG - Chr2:22783169-22783188 None:intergenic 25.0%
! CTTCAACAATTTCAATACTT+TGG - Chr2:22782865-22782884 None:intergenic 25.0%
! TATGTCTTTCTATGGTTTAA+TGG + Chr2:22783459-22783478 MsG0280007908.01.T01:intron 25.0%
!! AAAATAATTTTACACCACAG+TGG - Chr2:22783489-22783508 None:intergenic 25.0%
!! ATACTTATATTGGTAGTTTC+AGG + Chr2:22783321-22783340 MsG0280007908.01.T01:intron 25.0%
!!! AATAGCCTTATTTGGTATTA+TGG - Chr2:22783537-22783556 None:intergenic 25.0%
!!! AATTAACCGTCTTTATTTTC+AGG - Chr2:22783049-22783068 None:intergenic 25.0%
!!! CAAAGTATTGAAATTGTTGA+AGG + Chr2:22782863-22782882 MsG0280007908.01.T01:CDS 25.0%
!!! TTGTTTCCCATATTGTTTTA+CGG - Chr2:22783354-22783373 None:intergenic 25.0%
AAACCATAGAAAGACATAGA+TGG - Chr2:22783457-22783476 None:intergenic 30.0%
AACGAAAGTAAGTTTCTTGA+TGG - Chr2:22782906-22782925 None:intergenic 30.0%
AACGTATCAATTAAATGTGC+AGG + Chr2:22783952-22783971 MsG0280007908.01.T01:intron 30.0%
AAGAAACTTACTTTCGTTGA+AGG + Chr2:22782908-22782927 MsG0280007908.01.T01:CDS 30.0%
AGCTATATGCTTTAGTAATC+AGG - Chr2:22784258-22784277 None:intergenic 30.0%
AGTAACTATATCGATCGATT+TGG + Chr2:22783204-22783223 MsG0280007908.01.T01:intron 30.0%
ATACTAGAGCATGTTTAAGA+TGG + Chr2:22783092-22783111 MsG0280007908.01.T01:intron 30.0%
CCATATCCTGAAAATAAAGA+CGG + Chr2:22783040-22783059 MsG0280007908.01.T01:intron 30.0%
TTACCATCTATGTCTTTCTA+TGG + Chr2:22783451-22783470 MsG0280007908.01.T01:intron 30.0%
TTTCACAATCACAAACATCA+TGG - Chr2:22783934-22783953 None:intergenic 30.0%
! AAAGTATGATTTGCACAAAG+TGG + Chr2:22783984-22784003 MsG0280007908.01.T01:CDS 30.0%
! TACTAGAGCATGTTTAAGAT+GGG + Chr2:22783093-22783112 MsG0280007908.01.T01:intron 30.0%
!! ATCAATAACCTTTGGGATAA+GGG - Chr2:22782837-22782856 None:intergenic 30.0%
ACTGTGAAATACTTCACCAA+AGG + Chr2:22784141-22784160 MsG0280007908.01.T01:CDS 35.0%
ATGAGATTACTCTCAACATG+CGG + Chr2:22783291-22783310 MsG0280007908.01.T01:intron 35.0%
CGGCTACCAAATACTTATAT+TGG + Chr2:22783311-22783330 MsG0280007908.01.T01:intron 35.0%
GAATTGTCATAGTTGTCACA+CGG - Chr2:22783513-22783532 None:intergenic 35.0%
GTATCAATTAAATGTGCAGG+TGG + Chr2:22783955-22783974 MsG0280007908.01.T01:intron 35.0%
TGCAGACAATTTAGACTCAA+AGG - Chr2:22784091-22784110 None:intergenic 35.0%
TGCATGCAGTGAAAATTGTA+TGG - Chr2:22783018-22783037 None:intergenic 35.0%
TTGAGTCTAAATTGTCTGCA+GGG + Chr2:22784091-22784110 MsG0280007908.01.T01:CDS 35.0%
TTGCTTGCTTGCTTTATACA+TGG + Chr2:22784280-22784299 MsG0280007908.01.T01:three_prime_UTR 35.0%
TTTCACAGTAAGCTTTGCAA+TGG - Chr2:22784130-22784149 None:intergenic 35.0%
TTTGAGTCTAAATTGTCTGC+AGG + Chr2:22784090-22784109 MsG0280007908.01.T01:CDS 35.0%
! AGTAAGTTTCTTGATGGTTC+CGG - Chr2:22782900-22782919 None:intergenic 35.0%
!! CATCAATAACCTTTGGGATA+AGG - Chr2:22782838-22782857 None:intergenic 35.0%
!! GGAGATCACTTAGTTCTATT+AGG - Chr2:22783913-22783932 None:intergenic 35.0%
!!! CCGTCTTTATTTTCAGGATA+TGG - Chr2:22783043-22783062 None:intergenic 35.0%
ACCAAGTGAAGAGGAAATCA+AGG + Chr2:22784173-22784192 MsG0280007908.01.T01:CDS 40.0%
CCAAGTGAAGAGGAAATCAA+GGG + Chr2:22784174-22784193 MsG0280007908.01.T01:CDS 40.0%
GAAATTGTTGAAGGAAACGG+TGG + Chr2:22782872-22782891 MsG0280007908.01.T01:CDS 40.0%
GCTTACAACTACAGCATAGT+TGG + Chr2:22784030-22784049 MsG0280007908.01.T01:CDS 40.0%
GGATGGCATCAATAACCTTT+GGG - Chr2:22782844-22782863 None:intergenic 40.0%
TACAACTACAGCATAGTTGG+TGG + Chr2:22784033-22784052 MsG0280007908.01.T01:CDS 40.0%
TGGATGGCATCAATAACCTT+TGG - Chr2:22782845-22782864 None:intergenic 40.0%
TGTTGAGAGTAATCTCATCG+CGG - Chr2:22783289-22783308 None:intergenic 40.0%
TTCTGATACCCTTATCCCAA+AGG + Chr2:22782826-22782845 MsG0280007908.01.T01:CDS 40.0%
! AGAGCTTTGTAAAGTCTAGC+AGG - Chr2:22782797-22782816 None:intergenic 40.0%
!! TAGAGGTGATGGTCTTTTCA+AGG + Chr2:22784206-22784225 MsG0280007908.01.T01:CDS 40.0%
AGCAGGAGCTACAATAGATG+TGG - Chr2:22782780-22782799 None:intergenic 45.0%
AGTGAAGAGGAAATCAAGGG+TGG + Chr2:22784177-22784196 MsG0280007908.01.T01:CDS 45.0%
CATGGGTGTCTTCAACTTTG+AGG + Chr2:22782748-22782767 MsG0280007908.01.T01:exon 45.0%
CCCTTGATTTCCTCTTCACT+TGG - Chr2:22784177-22784196 None:intergenic 45.0%
GGTTTAATGGAGATCCACTG+TGG + Chr2:22783472-22783491 MsG0280007908.01.T01:intron 45.0%
TTTGCAATGGATCCTCCATC+TGG - Chr2:22784117-22784136 None:intergenic 45.0%
! CATGTTTAAGATGGGTCGAC+TGG + Chr2:22783101-22783120 MsG0280007908.01.T01:intron 45.0%
! GGCTCTTGAAGGTTACGTTT+TGG + Chr2:22784227-22784246 MsG0280007908.01.T01:CDS 45.0%
!! AACTACAGCATAGTTGGTGG+TGG + Chr2:22784036-22784055 MsG0280007908.01.T01:CDS 45.0%
!! GGTCTTTTCAAGGCTCTTGA+AGG + Chr2:22784216-22784235 MsG0280007908.01.T01:CDS 45.0%
!! AATATATAATAAAATAAAAG+AGG - Chr2:22783683-22783702 None:intergenic 5.0%
!! ATATATAATAAAATAAAAGA+GGG - Chr2:22783682-22783701 None:intergenic 5.0%
!!! TCTTTTATTTTATTATATAT+TGG + Chr2:22783682-22783701 MsG0280007908.01.T01:intron 5.0%
AAATTGTCTGCAGGGCCAGA+TGG + Chr2:22784099-22784118 MsG0280007908.01.T01:CDS 50.0%
ATCAAGGGTGGCAAAGCTAG+AGG + Chr2:22784189-22784208 MsG0280007908.01.T01:CDS 50.0%
GAGATCTTCTCCACTGTGTC+TGG - Chr2:22784069-22784088 None:intergenic 50.0%
TGATGCTGCACCAAGTGAAG+AGG + Chr2:22784164-22784183 MsG0280007908.01.T01:CDS 50.0%
!! ACTTGGTGCAGCATCACCTT+TGG - Chr2:22784160-22784179 None:intergenic 50.0%
!! TACAGCATAGTTGGTGGTGG+TGG + Chr2:22784039-22784058 MsG0280007908.01.T01:CDS 50.0%
GGTGGCAAAGCTAGAGGTGA+TGG + Chr2:22784195-22784214 MsG0280007908.01.T01:CDS 55.0%
!! TGGTGGACTTCCAGACACAG+TGG + Chr2:22784056-22784075 MsG0280007908.01.T01:CDS 55.0%
GTTGAAGGAAACGGTGGCGC+CGG + Chr2:22782878-22782897 MsG0280007908.01.T01:CDS 60.0%
TTGTCTGCAGGGCCAGATGG+AGG + Chr2:22784102-22784121 MsG0280007908.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 22782678 22784309 22782678 ID=MsG0280007908.01;Name=MsG0280007908.01
Chr2 mRNA 22782678 22784309 22782678 ID=MsG0280007908.01.T01;Parent=MsG0280007908.01;Name=MsG0280007908.01.T01;_AED=0.49;_eAED=0.49;_QI=71|1|1|1|1|1|2|43|157
Chr2 exon 22782678 22782929 22782678 ID=MsG0280007908.01.T01:exon:13868;Parent=MsG0280007908.01.T01
Chr2 exon 22783974 22784309 22783974 ID=MsG0280007908.01.T01:exon:13869;Parent=MsG0280007908.01.T01
Chr2 five_prime_UTR 22782678 22782748 22782678 ID=MsG0280007908.01.T01:five_prime_utr;Parent=MsG0280007908.01.T01
Chr2 CDS 22782749 22782929 22782749 ID=MsG0280007908.01.T01:cds;Parent=MsG0280007908.01.T01
Chr2 CDS 22783974 22784266 22783974 ID=MsG0280007908.01.T01:cds;Parent=MsG0280007908.01.T01
Chr2 three_prime_UTR 22784267 22784309 22784267 ID=MsG0280007908.01.T01:three_prime_utr;Parent=MsG0280007908.01.T01
Gene Sequence

>MsG0280007908.01.T01

ATGGGTGTCTTCAACTTTGAGGATGAAACCACATCTATTGTAGCTCCTGCTAGACTTTACAAAGCTCTAGTTACAGATTCTGATACCCTTATCCCAAAGGTTATTGATGCCATCCAAAGTATTGAAATTGTTGAAGGAAACGGTGGCGCCGGAACCATCAAGAAACTTACTTTCGTTGAAGGTGGTGAAACAAAGTATGATTTGCACAAAGTGGACTTAGTAGATGATGTTAACTTTGCTTACAACTACAGCATAGTTGGTGGTGGTGGACTTCCAGACACAGTGGAGAAGATCTCCTTTGAGTCTAAATTGTCTGCAGGGCCAGATGGAGGATCCATTGCAAAGCTTACTGTGAAATACTTCACCAAAGGTGATGCTGCACCAAGTGAAGAGGAAATCAAGGGTGGCAAAGCTAGAGGTGATGGTCTTTTCAAGGCTCTTGAAGGTTACGTTTTGGCAAATCCTGATTACTAA

Protein sequence

>MsG0280007908.01.T01

MGVFNFEDETTSIVAPARLYKALVTDSDTLIPKVIDAIQSIEIVEGNGGAGTIKKLTFVEGGETKYDLHKVDLVDDVNFAYNYSIVGGGGLPDTVEKISFESKLSAGPDGGSIAKLTVKYFTKGDAAPSEEEIKGGKARGDGLFKALEGYVLANPDY*