AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003322.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003322.01.T01 MTR_1g058560 98.462 195 3 0 1 195 1 195 6.42e-138 383
MsG0180003322.01.T01 MTR_1g058540 81.633 196 35 1 1 195 1 196 7.14e-100 287
MsG0180003322.01.T01 MTR_1g058570 88.205 195 22 1 1 195 1 194 1.37e-99 286
MsG0180003322.01.T01 MTR_2g086740 60.694 173 68 0 22 194 47 219 7.80e-59 184
MsG0180003322.01.T01 MTR_7g011090 52.941 170 80 0 26 195 17 186 4.35e-56 176
MsG0180003322.01.T01 MTR_4g081880 42.932 191 103 2 4 194 2 186 7.13e-51 162
MsG0180003322.01.T01 MTR_5g041900 48.503 167 86 0 29 195 20 186 5.25e-45 147
MsG0180003322.01.T01 MTR_2g086740 63.462 104 38 0 22 125 47 150 2.30e-34 119
MsG0180003322.01.T01 MTR_0004s0210 37.419 155 84 4 36 188 7 150 5.35e-21 85.5
MsG0180003322.01.T01 MTR_0097s0070 33.898 118 76 2 72 188 69 185 6.58e-14 67.4
MsG0180003322.01.T01 MTR_4g118800 30.833 120 82 1 74 192 62 181 1.90e-12 63.2
MsG0180003322.01.T01 MTR_8g089300 29.487 156 101 3 41 188 30 184 1.94e-12 63.5
MsG0180003322.01.T01 MTR_7g108130 27.742 155 109 2 36 188 28 181 2.52e-12 63.2
MsG0180003322.01.T01 MTR_3g064310 32.203 118 77 2 75 192 77 191 3.15e-12 63.2
MsG0180003322.01.T01 MTR_5g076480 34.483 116 72 3 75 189 69 181 4.33e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003322.01.T01 AT2G36100 56.863 204 76 3 2 195 5 206 1.32e-70 213
MsG0180003322.01.T01 AT3G11550 62.189 201 68 3 2 195 5 204 3.32e-64 197
MsG0180003322.01.T01 AT5G06200 61.616 198 70 3 2 194 5 201 1.06e-63 196
MsG0180003322.01.T01 AT2G27370 51.724 203 83 2 7 194 17 219 2.07e-62 193
MsG0180003322.01.T01 AT5G15290 44.828 174 86 1 2 175 3 166 1.98e-49 159
MsG0180003322.01.T01 AT1G14160 41.212 165 97 0 31 195 45 209 4.13e-44 146
MsG0180003322.01.T01 AT1G03700 32.886 149 91 2 40 188 12 151 2.56e-19 81.3
MsG0180003322.01.T01 AT1G17200 27.660 188 124 4 9 192 2 181 2.05e-16 74.3
MsG0180003322.01.T01 AT4G03540 33.058 121 72 2 56 176 28 139 2.36e-15 70.9
MsG0180003322.01.T01 AT5G44550 33.951 162 95 4 41 194 22 179 2.74e-15 71.2
MsG0180003322.01.T01 AT3G06390 30.075 133 88 2 37 164 37 169 2.84e-13 65.9
MsG0180003322.01.T01 AT4G15630 26.601 203 118 6 2 192 6 189 7.90e-12 61.6
MsG0180003322.01.T01 AT4G15610 26.144 153 107 3 28 175 20 171 8.18e-12 61.6

Find 40 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATCCTCCTGGCCTTGC+AGG 0.176057 1:+60616843 None:intergenic
TGATTCTTGGATATCAATTT+TGG 0.215560 1:+60616905 None:intergenic
CTAGCCATTTGCAACCAATT+TGG 0.242258 1:-60616026 MsG0180003322.01.T01:CDS
GTGATGTCTGCTTTGGCTAT+TGG 0.247792 1:-60615930 MsG0180003322.01.T01:CDS
TGGTATCTTTGCTTGATTCT+TGG 0.263645 1:+60616892 None:intergenic
GGCAATAGTGGCTGGCTATT+TGG 0.274909 1:-60616489 MsG0180003322.01.T01:CDS
TGATGTCTGCTTTGGCTATT+GGG 0.288501 1:-60615929 MsG0180003322.01.T01:CDS
CCTTAAAATGAAATCCATTA+TGG 0.301071 1:+60616809 None:intergenic
TACCAAAGGAAAAGCTGTTT+TGG 0.315492 1:-60616874 MsG0180003322.01.T01:CDS
ATGATGGCTACTATAGAAAA+AGG 0.341980 1:+60616455 None:intergenic
AAGGCCAGGAGGATGGAAAA+AGG 0.353235 1:-60616838 MsG0180003322.01.T01:CDS
TCTTATTGCAATGGCAATAG+TGG 0.367241 1:-60616501 MsG0180003322.01.T01:CDS
TCTTGCCGCTGCCGCCTCGA+TGG 0.384071 1:-60616766 MsG0180003322.01.T01:CDS
AGCTGCTTCCATAGTTTACT+TGG 0.385601 1:-60616078 MsG0180003322.01.T01:CDS
CTTGCCGCTGCCGCCTCGAT+GGG 0.402133 1:-60616765 MsG0180003322.01.T01:CDS
GTTGGTTGTGATGTCTGCTT+TGG 0.406589 1:-60615937 MsG0180003322.01.T01:CDS
GTGCACAGAAATCTCCAAAT+TGG 0.409853 1:+60616012 None:intergenic
ATGGAAATCAAGACACAAAC+TGG 0.414877 1:-60616049 MsG0180003322.01.T01:CDS
AGCGGCAAGAGCTGCTGCTA+TGG 0.432197 1:+60616779 None:intergenic
ATCTCCAAATTGGTTGCAAA+TGG 0.445100 1:+60616022 None:intergenic
GTTGCAGCACCTGCAAGGCC+AGG 0.457764 1:-60616852 MsG0180003322.01.T01:CDS
CACCTGCAAGGCCAGGAGGA+TGG 0.475215 1:-60616845 MsG0180003322.01.T01:CDS
ATTGTGTGCCAAGTAAACTA+TGG 0.493367 1:+60616070 None:intergenic
AATGTTACATACTGGAATGC+AGG 0.494232 1:+60616675 None:intergenic
AAGCCTTGGTCCAGTTGCAT+GGG 0.497314 1:+60616427 None:intergenic
TGTTCCCATCGAGGCGGCAG+CGG 0.517932 1:+60616761 None:intergenic
AAAGCCTTGGTCCAGTTGCA+TGG 0.518863 1:+60616426 None:intergenic
ATTGCAGGTATTTCTTACAC+TGG 0.547240 1:-60616120 MsG0180003322.01.T01:intron
ATTGCAATGGCAATAGTGGC+TGG 0.552076 1:-60616497 MsG0180003322.01.T01:CDS
ATTGAGATCTTACAGTGTCT+AGG 0.588191 1:+60616393 None:intergenic
TGTGGCATCATTTATTACTG+TGG 0.599611 1:-60615973 MsG0180003322.01.T01:CDS
ACAGACAAGTGGTGCAGTTG+TGG 0.601968 1:-60615991 MsG0180003322.01.T01:CDS
AGCCTTGGTCCAGTTGCATG+GGG 0.616293 1:+60616428 None:intergenic
ATAGTTTACTTGGCACACAA+TGG 0.619739 1:-60616068 MsG0180003322.01.T01:CDS
GCAGCACCTGCAAGGCCAGG+AGG 0.636977 1:-60616849 MsG0180003322.01.T01:CDS
GAATCAAGCAAAGATACCAA+AGG 0.648211 1:-60616888 MsG0180003322.01.T01:CDS
ATCACTTGTTCCCATCGAGG+CGG 0.653937 1:+60616755 None:intergenic
TTGATCACTTGTTCCCATCG+AGG 0.661241 1:+60616752 None:intergenic
GATTTCTGTGCACAGACAAG+TGG 0.683686 1:-60616002 MsG0180003322.01.T01:CDS
GCCTTGGTCCAGTTGCATGG+GGG 0.707472 1:+60616429 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTCAAATATAAGTAAAAGTT+AGG - Chr1:60616518-60616537 MsG0180003322.01.T01:intron 20.0%
!!! GATGTATTTAATTCAACTTT+TGG + Chr1:60616568-60616587 None:intergenic 20.0%
!!! TTAGTTATGATTTTTCTTTG+TGG - Chr1:60616681-60616700 MsG0180003322.01.T01:intron 20.0%
! CCTTAAAATGAAATCCATTA+TGG + Chr1:60616026-60616045 None:intergenic 25.0%
! TTCTAAGTCACATATAAGAT+CGG - Chr1:60616202-60616221 MsG0180003322.01.T01:intron 25.0%
!! TGATTCTTGGATATCAATTT+TGG + Chr1:60615930-60615949 None:intergenic 25.0%
!!! ATGGATTTCATTTTAAGGTT+AGG - Chr1:60616028-60616047 MsG0180003322.01.T01:CDS 25.0%
!!! CCATAATGGATTTCATTTTA+AGG - Chr1:60616023-60616042 MsG0180003322.01.T01:CDS 25.0%
!!! CTTTTACTTATATTTGAGAC+TGG + Chr1:60616516-60616535 None:intergenic 25.0%
!!! TCAACAAAAGTTGATGTATT+TGG - Chr1:60616541-60616560 MsG0180003322.01.T01:intron 25.0%
ACTTAATGCTTGAAAAATGC+AGG - Chr1:60616303-60616322 MsG0180003322.01.T01:intron 30.0%
ATGATGGCTACTATAGAAAA+AGG + Chr1:60616380-60616399 None:intergenic 30.0%
TAGATGTTTACTACTCTCTT+CGG + Chr1:60616627-60616646 None:intergenic 30.0%
! TACTTATATTTGAGACTGGA+GGG + Chr1:60616512-60616531 None:intergenic 30.0%
! TTACTTATATTTGAGACTGG+AGG + Chr1:60616513-60616532 None:intergenic 30.0%
!!! CAGGTTTTTTCTTATTGCAA+TGG - Chr1:60616322-60616341 MsG0180003322.01.T01:intron 30.0%
!!! GAGTTTTGAATGTTACATAC+TGG + Chr1:60616168-60616187 None:intergenic 30.0%
!!! TGTGGTTGTTTTAATCTTGT+TGG - Chr1:60616877-60616896 MsG0180003322.01.T01:CDS 30.0%
AATGTTACATACTGGAATGC+AGG + Chr1:60616160-60616179 None:intergenic 35.0%
ATAGTTTACTTGGCACACAA+TGG - Chr1:60616764-60616783 MsG0180003322.01.T01:CDS 35.0%
ATCTCCAAATTGGTTGCAAA+TGG + Chr1:60616813-60616832 None:intergenic 35.0%
ATGGAAATCAAGACACAAAC+TGG - Chr1:60616783-60616802 MsG0180003322.01.T01:CDS 35.0%
ATTGAGATCTTACAGTGTCT+AGG + Chr1:60616442-60616461 None:intergenic 35.0%
ATTGCAGGTATTTCTTACAC+TGG - Chr1:60616712-60616731 MsG0180003322.01.T01:CDS 35.0%
ATTGTGTGCCAAGTAAACTA+TGG + Chr1:60616765-60616784 None:intergenic 35.0%
GAATCAAGCAAAGATACCAA+AGG - Chr1:60615944-60615963 MsG0180003322.01.T01:CDS 35.0%
TCTTATTGCAATGGCAATAG+TGG - Chr1:60616331-60616350 MsG0180003322.01.T01:intron 35.0%
TGGTATCTTTGCTTGATTCT+TGG + Chr1:60615943-60615962 None:intergenic 35.0%
TGTGGCATCATTTATTACTG+TGG - Chr1:60616859-60616878 MsG0180003322.01.T01:CDS 35.0%
TTTGTGGTGCTTATAATTGC+AGG - Chr1:60616697-60616716 MsG0180003322.01.T01:CDS 35.0%
! AACCAAAACAGCTTTTCCTT+TGG + Chr1:60615963-60615982 None:intergenic 35.0%
! ACTTATATTTGAGACTGGAG+GGG + Chr1:60616511-60616530 None:intergenic 35.0%
! TACCAAAGGAAAAGCTGTTT+TGG - Chr1:60615958-60615977 MsG0180003322.01.T01:CDS 35.0%
AGCTGCTTCCATAGTTTACT+TGG - Chr1:60616754-60616773 MsG0180003322.01.T01:CDS 40.0%
AGGATGATGAGAAAAAGCCT+TGG + Chr1:60616422-60616441 None:intergenic 40.0%
CTAGCCATTTGCAACCAATT+TGG - Chr1:60616806-60616825 MsG0180003322.01.T01:CDS 40.0%
GTGCACAGAAATCTCCAAAT+TGG + Chr1:60616823-60616842 None:intergenic 40.0%
TGATGTCTGCTTTGGCTATT+GGG - Chr1:60616903-60616922 MsG0180003322.01.T01:CDS 40.0%
! CTTATATTTGAGACTGGAGG+GGG + Chr1:60616510-60616529 None:intergenic 40.0%
! GAAAAAGGGCATAGCCATAA+TGG - Chr1:60616009-60616028 MsG0180003322.01.T01:CDS 40.0%
ATTGCAATGGCAATAGTGGC+TGG - Chr1:60616335-60616354 MsG0180003322.01.T01:intron 45.0%
GATTTCTGTGCACAGACAAG+TGG - Chr1:60616830-60616849 MsG0180003322.01.T01:CDS 45.0%
GTGATGTCTGCTTTGGCTAT+TGG - Chr1:60616902-60616921 MsG0180003322.01.T01:CDS 45.0%
GTTGGTTGTGATGTCTGCTT+TGG - Chr1:60616895-60616914 MsG0180003322.01.T01:CDS 45.0%
TTGATCACTTGTTCCCATCG+AGG + Chr1:60616083-60616102 None:intergenic 45.0%
AAAGCCTTGGTCCAGTTGCA+TGG + Chr1:60616409-60616428 None:intergenic 50.0%
AAGCCTTGGTCCAGTTGCAT+GGG + Chr1:60616408-60616427 None:intergenic 50.0%
AAGGCCAGGAGGATGGAAAA+AGG - Chr1:60615994-60616013 MsG0180003322.01.T01:CDS 50.0%
ACAGACAAGTGGTGCAGTTG+TGG - Chr1:60616841-60616860 MsG0180003322.01.T01:CDS 50.0%
AGGCCAGGAGGATGGAAAAA+GGG - Chr1:60615995-60616014 MsG0180003322.01.T01:CDS 50.0%
ATCACTTGTTCCCATCGAGG+CGG + Chr1:60616080-60616099 None:intergenic 50.0%
! ATGCCCTTTTTCCATCCTCC+TGG + Chr1:60616001-60616020 None:intergenic 50.0%
! GGCAATAGTGGCTGGCTATT+TGG - Chr1:60616343-60616362 MsG0180003322.01.T01:intron 50.0%
!! TTTTGGTTGCAGCACCTGCA+AGG - Chr1:60615975-60615994 MsG0180003322.01.T01:CDS 50.0%
AGCCTTGGTCCAGTTGCATG+GGG + Chr1:60616407-60616426 None:intergenic 55.0%
ATCATACGCCCCCATGCAAC+TGG - Chr1:60616395-60616414 MsG0180003322.01.T01:intron 55.0%
! AGTTGCATGGGGGCGTATGA+TGG + Chr1:60616396-60616415 None:intergenic 55.0%
AGCGGCAAGAGCTGCTGCTA+TGG + Chr1:60616056-60616075 None:intergenic 60.0%
GCCTTGGTCCAGTTGCATGG+GGG + Chr1:60616406-60616425 None:intergenic 60.0%
!! TTCCATCCTCCTGGCCTTGC+AGG + Chr1:60615992-60616011 None:intergenic 60.0%
CACCTGCAAGGCCAGGAGGA+TGG - Chr1:60615987-60616006 MsG0180003322.01.T01:CDS 65.0%
GCCCCCATGCAACTGGACCA+AGG - Chr1:60616402-60616421 MsG0180003322.01.T01:intron 65.0%
GTTGCAGCACCTGCAAGGCC+AGG - Chr1:60615980-60615999 MsG0180003322.01.T01:CDS 65.0%
TGTTCCCATCGAGGCGGCAG+CGG + Chr1:60616074-60616093 None:intergenic 65.0%
CTTGCCGCTGCCGCCTCGAT+GGG - Chr1:60616067-60616086 MsG0180003322.01.T01:CDS 70.0%
GCAGCACCTGCAAGGCCAGG+AGG - Chr1:60615983-60616002 MsG0180003322.01.T01:CDS 70.0%
TCTTGCCGCTGCCGCCTCGA+TGG - Chr1:60616066-60616085 MsG0180003322.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 60615920 60616934 60615920 ID=MsG0180003322.01;Name=MsG0180003322.01
Chr1 mRNA 60615920 60616934 60615920 ID=MsG0180003322.01.T01;Parent=MsG0180003322.01;Name=MsG0180003322.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|3|0|195
Chr1 exon 60616687 60616934 60616687 ID=MsG0180003322.01.T01:exon:4077;Parent=MsG0180003322.01.T01
Chr1 exon 60616406 60616529 60616406 ID=MsG0180003322.01.T01:exon:4076;Parent=MsG0180003322.01.T01
Chr1 exon 60615920 60616135 60615920 ID=MsG0180003322.01.T01:exon:4075;Parent=MsG0180003322.01.T01
Chr1 CDS 60616687 60616934 60616687 ID=MsG0180003322.01.T01:cds;Parent=MsG0180003322.01.T01
Chr1 CDS 60616406 60616529 60616406 ID=MsG0180003322.01.T01:cds;Parent=MsG0180003322.01.T01
Chr1 CDS 60615920 60616135 60615920 ID=MsG0180003322.01.T01:cds;Parent=MsG0180003322.01.T01
Gene Sequence

>MsG0180003322.01.T01

ATGTCAACCAAAATTGATATCCAAGAATCAAGCAAAGATACCAAAGGAAAAGCTGTTTTGGTTGCAGCACCTGCAAGGCCAGGAGGATGGAAAAAGGGCATAGCCATAATGGATTTCATTTTAAGGTTAGGTGCCATAGCAGCAGCTCTTGCCGCTGCCGCCTCGATGGGAACAAGTGATCAATCTCTTTCTTTCTTTACTCAGTTCTTTCAGTTTGAAGCTAGCTATGATAGCTTTCCTGCATTCCAGTTTTTTCTTATTGCAATGGCAATAGTGGCTGGCTATTTGGTCTTGTCTCTACCTTTTTCTATAGTAGCCATCATACGCCCCCATGCAACTGGACCAAGGCTTTTTCTCATCATCCTAGACACTGTATTTCTTACACTGGCTACTGCTAGTGCTGCTTCAGCTGCTTCCATAGTTTACTTGGCACACAATGGAAATCAAGACACAAACTGGCTAGCCATTTGCAACCAATTTGGAGATTTCTGTGCACAGACAAGTGGTGCAGTTGTGGCATCATTTATTACTGTGGTTGTTTTAATCTTGTTGGTTGTGATGTCTGCTTTGGCTATTGGGAAGCATTGA

Protein sequence

>MsG0180003322.01.T01

MSTKIDIQESSKDTKGKAVLVAAPARPGGWKKGIAIMDFILRLGAIAAALAAAASMGTSDQSLSFFTQFFQFEASYDSFPAFQFFLIAMAIVAGYLVLSLPFSIVAIIRPHATGPRLFLIILDTVFLTLATASAASAASIVYLAHNGNQDTNWLAICNQFGDFCAQTSGAVVASFITVVVLILLVVMSALAIGKH*