Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015146.01.T01 | XP_003600586.1 | 98.592 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 2.46E-42 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015146.01.T01 | G7J780 | 98.592 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 1.17e-42 | 142 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049121.01 | MsG0380015146.01 | 0.807478 | 4.956203e-50 | 2.763433e-47 |
| MsG0180002489.01 | MsG0380015146.01 | 0.813775 | 2.157350e-51 | 1.421733e-48 |
| MsG0280006411.01 | MsG0380015146.01 | 0.829758 | 4.303204e-55 | 4.446100e-52 |
| MsG0280006422.01 | MsG0380015146.01 | 0.805979 | 1.027445e-49 | 5.512015e-47 |
| MsG0380015146.01 | MsG0380015157.01 | 0.812475 | 4.161549e-51 | 2.648710e-48 |
| MsG0380015146.01 | MsG0380016133.01 | 0.805896 | 1.069637e-49 | 5.726029e-47 |
| MsG0380015146.01 | MsG0480018110.01 | 0.814788 | 1.288861e-51 | 8.730034e-49 |
| MsG0380015146.01 | MsG0480021448.01 | 0.825046 | 5.801795e-54 | 5.229985e-51 |
| MsG0380015146.01 | MsG0480021453.01 | 0.828815 | 7.290322e-55 | 7.325672e-52 |
| MsG0380015146.01 | MsG0480021464.01 | 0.818289 | 2.118169e-52 | 1.578977e-49 |
| MsG0380015146.01 | MsG0480022223.01 | 0.807576 | 4.724121e-50 | 2.640587e-47 |
| MsG0380015146.01 | MsG0480022696.01 | 0.800377 | 1.482800e-48 | 6.893897e-46 |
| MsG0380015146.01 | MsG0480023088.01 | 0.804379 | 2.222592e-49 | 1.144234e-46 |
| MsG0380015146.01 | MsG0580024051.01 | 0.826511 | 2.606077e-54 | 2.449109e-51 |
| MsG0380015146.01 | MsG0580025134.01 | 0.805429 | 1.340682e-49 | 7.091225e-47 |
| MsG0380015146.01 | MsG0580025756.01 | 0.806459 | 8.141383e-50 | 4.421450e-47 |
| MsG0380015146.01 | MsG0580026047.01 | 0.817866 | 2.640233e-52 | 1.945442e-49 |
| MsG0380015146.01 | MsG0580028284.01 | 0.804267 | 2.345112e-49 | 1.203803e-46 |
| MsG0380015146.01 | MsG0580028289.01 | 0.808713 | 2.705405e-50 | 1.557933e-47 |
| MsG0380015146.01 | MsG0680030366.01 | 0.822827 | 1.923143e-53 | 1.627677e-50 |
| MsG0380015146.01 | MsG0680030415.01 | 0.806594 | 7.625451e-50 | 4.155254e-47 |
| MsG0380015146.01 | MsG0680030459.01 | 0.814216 | 1.724649e-51 | 1.150238e-48 |
| MsG0380015146.01 | MsG0780038911.01 | 0.823356 | 1.447493e-53 | 1.243348e-50 |
| MsG0380015146.01 | MsG0780040545.01 | 0.831694 | 1.443852e-55 | 1.578841e-52 |
| MsG0380015146.01 | MsG0780040858.01 | 0.814115 | 1.815287e-51 | 1.207403e-48 |
| MsG0380015146.01 | MsG0880043456.01 | 0.802911 | 4.482000e-49 | 2.222143e-46 |
| MsG0380015146.01 | MsG0880043465.01 | 0.813901 | 2.023721e-51 | 1.338315e-48 |
| MsG0380015146.01 | MsG0880043477.01 | 0.808420 | 3.124232e-50 | 1.785546e-47 |
| MsG0380015146.01 | MsG0880045965.01 | 0.816405 | 5.624312e-52 | 3.981637e-49 |
| MsG0380015146.01 | MsG0880046089.01 | 0.805051 | 1.608603e-49 | 8.426231e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015146.01.T01 | MTR_3g063970 | 98.592 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 2.98e-46 | 142 |
| MsG0380015146.01.T01 | MTR_3g063970 | 97.917 | 48 | 1 | 0 | 1 | 48 | 1 | 48 | 6.63e-16 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015146.01.T01 | AT3G14430 | 65.079 | 63 | 21 | 1 | 9 | 71 | 15 | 76 | 1.08e-24 | 87.8 |
Find 28 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACATCAATTCCTTTAAA+AGG | 0.150864 | 3:-65878929 | None:intergenic |
| ATTTCTTACGAAGGCGAAAA+TGG | 0.322719 | 3:-65877931 | None:intergenic |
| GTTAATGTTACATTCTAGTT+AGG | 0.334854 | 3:+65879125 | MsG0380015146.01.T01:three_prime_UTR |
| ACAAATACTTGATTGTATTC+AGG | 0.336136 | 3:-65878874 | None:intergenic |
| GTATCTTTATGTAAGTTTGT+AGG | 0.358607 | 3:+65879068 | MsG0380015146.01.T01:three_prime_UTR |
| CTGGTGGTGGACCAGAGCTT+TGG | 0.389186 | 3:+65878905 | MsG0380015146.01.T01:CDS |
| CTTTAAAAGGGCCAAAGCTC+TGG | 0.402112 | 3:-65878916 | None:intergenic |
| AAACATCAATTCCTTTAAAA+GGG | 0.443719 | 3:-65878928 | None:intergenic |
| AAAGGAATTGATGTTTGGAC+AGG | 0.475928 | 3:+65878935 | MsG0380015146.01.T01:exon |
| CAAAGCTCTGGTCCACCACC+AGG | 0.488950 | 3:-65878904 | None:intergenic |
| CAAGTATTTGTGAAGAAGCC+TGG | 0.508253 | 3:+65878886 | MsG0380015146.01.T01:CDS |
| CATAAAGATACAAATACAAA+CGG | 0.528427 | 3:-65879056 | None:intergenic |
| GAATTGATGTTTGGACAGGT+TGG | 0.530812 | 3:+65878939 | MsG0380015146.01.T01:three_prime_UTR |
| GACAGGTTGGATGTCATCCA+TGG | 0.532988 | 3:+65878952 | MsG0380015146.01.T01:three_prime_UTR |
| CAGAGAGAAGAAGACTAGAC+TGG | 0.543222 | 3:+65877817 | MsG0380015146.01.T01:five_prime_UTR |
| TGAAGAAGAAAAGAAAGAAG+AGG | 0.565354 | 3:-65877968 | None:intergenic |
| AGAGAACAGCGATGGCTAGA+CGG | 0.584999 | 3:+65877862 | MsG0380015146.01.T01:exon |
| CTAGACGGCGAGTCGAGACT+CGG | 0.585126 | 3:+65877877 | MsG0380015146.01.T01:CDS |
| GAAATATGTAAAATCATCAC+AGG | 0.585504 | 3:-65879034 | None:intergenic |
| AAAATATGTGAAATCATCAC+AGG | 0.600529 | 3:-65878993 | None:intergenic |
| GGAACGAGTCGTTATTGACT+CGG | 0.604165 | 3:+65877898 | MsG0380015146.01.T01:CDS |
| TTTGTGAAGAAGCCTGGTGG+TGG | 0.604245 | 3:+65878892 | MsG0380015146.01.T01:CDS |
| ATTAACATCATTCAAATCTG+TGG | 0.614330 | 3:-65879108 | None:intergenic |
| GAGGTGAGAGAGAACAGCGA+TGG | 0.620676 | 3:+65877854 | MsG0380015146.01.T01:exon |
| AAACTCAGCATTTCTTACGA+AGG | 0.638252 | 3:-65877940 | None:intergenic |
| GCGTGCTTAGAATACCTCCA+TGG | 0.702615 | 3:-65878969 | None:intergenic |
| AGGTTGGATGTCATCCATGG+AGG | 0.736701 | 3:+65878955 | MsG0380015146.01.T01:three_prime_UTR |
| GTATTTGTGAAGAAGCCTGG+TGG | 0.787083 | 3:+65878889 | MsG0380015146.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGGATAATTATATCAAAA+AGG | + | Chr3:65878201-65878220 | MsG0380015146.01.T01:intron | 15.0% |
| !!! | TTTTGATAAGTGAAAATTTT+TGG | + | Chr3:65878731-65878750 | MsG0380015146.01.T01:intron | 15.0% |
| !! | AGATTCACAAATTTATATGT+CGG | + | Chr3:65878437-65878456 | MsG0380015146.01.T01:intron | 20.0% |
| !! | ATAATTCATTCATATATGGT+TGG | + | Chr3:65878708-65878727 | MsG0380015146.01.T01:intron | 20.0% |
| !! | CATAAAGATACAAATACAAA+CGG | - | Chr3:65879059-65879078 | None:intergenic | 20.0% |
| !! | CTTCATAATTCATTCATATA+TGG | + | Chr3:65878704-65878723 | MsG0380015146.01.T01:intron | 20.0% |
| !! | TTAGTTACTAAATTGAAAAC+TGG | + | Chr3:65878167-65878186 | MsG0380015146.01.T01:intron | 20.0% |
| !!! | AAACATCAATTCCTTTAAAA+GGG | - | Chr3:65878931-65878950 | None:intergenic | 20.0% |
| !!! | CTTTTAATGTTTGAGAAATT+AGG | + | Chr3:65878117-65878136 | MsG0380015146.01.T01:intron | 20.0% |
| ! | AAAATATGTGAAATCATCAC+AGG | - | Chr3:65878996-65879015 | None:intergenic | 25.0% |
| ! | AACTATATACCAATTCCAAT+TGG | - | Chr3:65878241-65878260 | None:intergenic | 25.0% |
| ! | ACAAATACTTGATTGTATTC+AGG | - | Chr3:65878877-65878896 | None:intergenic | 25.0% |
| ! | ATCACAAAAAAAAAAGAGAG+AGG | - | Chr3:65878031-65878050 | None:intergenic | 25.0% |
| ! | ATTAACATCATTCAAATCTG+TGG | - | Chr3:65879111-65879130 | None:intergenic | 25.0% |
| ! | ATTTGAACAAATGATGCAAA+TGG | - | Chr3:65878064-65878083 | None:intergenic | 25.0% |
| ! | CAAACATCAATTCCTTTAAA+AGG | - | Chr3:65878932-65878951 | None:intergenic | 25.0% |
| ! | GAAATATGTAAAATCATCAC+AGG | - | Chr3:65879037-65879056 | None:intergenic | 25.0% |
| ! | GTATCTTTATGTAAGTTTGT+AGG | + | Chr3:65879068-65879087 | MsG0380015146.01.T01:three_prime_UTR | 25.0% |
| ! | TTAAATTACAATCAGCATGT+TGG | + | Chr3:65878504-65878523 | MsG0380015146.01.T01:intron | 25.0% |
| ! | TTCAATGTAATCATATGTGT+CGG | + | Chr3:65878629-65878648 | MsG0380015146.01.T01:intron | 25.0% |
| ! | TTTGAACAAATGATGCAAAT+GGG | - | Chr3:65878063-65878082 | None:intergenic | 25.0% |
| !! | GTTAATGTTACATTCTAGTT+AGG | + | Chr3:65879125-65879144 | MsG0380015146.01.T01:three_prime_UTR | 25.0% |
| !! | TTGTCTTGTAATTTCACTTT+TGG | + | Chr3:65878806-65878825 | MsG0380015146.01.T01:intron | 25.0% |
| !!! | AATTTTCGTTCTTTTTCATC+TGG | + | Chr3:65878081-65878100 | MsG0380015146.01.T01:intron | 25.0% |
| !!! | ATTTTCGTTCTTTTTCATCT+GGG | + | Chr3:65878082-65878101 | MsG0380015146.01.T01:intron | 25.0% |
| !!! | CTTTTAAAGGAATTGATGTT+TGG | + | Chr3:65878930-65878949 | MsG0380015146.01.T01:exon | 25.0% |
| ATTCTGTTTCCAATTGGAAT+TGG | + | Chr3:65878229-65878248 | MsG0380015146.01.T01:intron | 30.0% | |
| TGAAATTACAAGACAAACAG+AGG | - | Chr3:65878802-65878821 | None:intergenic | 30.0% | |
| TGAAGAAGAAAAGAAAGAAG+AGG | - | Chr3:65877971-65877990 | None:intergenic | 30.0% | |
| ! | ACTGGATTGTTAGAATTTTG+AGG | + | Chr3:65877835-65877854 | MsG0380015146.01.T01:five_prime_UTR | 30.0% |
| !! | AAACTGGTTTGATGATTGAT+TGG | + | Chr3:65878183-65878202 | MsG0380015146.01.T01:intron | 30.0% |
| AAACTCAGCATTTCTTACGA+AGG | - | Chr3:65877943-65877962 | None:intergenic | 35.0% | |
| AAAGGAATTGATGTTTGGAC+AGG | + | Chr3:65878935-65878954 | MsG0380015146.01.T01:exon | 35.0% | |
| AATTACAAGACAAACAGAGG+AGG | - | Chr3:65878799-65878818 | None:intergenic | 35.0% | |
| ACTGATGAAAACAACTTACG+AGG | - | Chr3:65878407-65878426 | None:intergenic | 35.0% | |
| ATTACAAGACAAACAGAGGA+GGG | - | Chr3:65878798-65878817 | None:intergenic | 35.0% | |
| ATTTCTTACGAAGGCGAAAA+TGG | - | Chr3:65877934-65877953 | None:intergenic | 35.0% | |
| GATTACGAGTCTGTTTGAAT+TGG | - | Chr3:65878481-65878500 | None:intergenic | 35.0% | |
| GTAGAGATTCTGTTTCCAAT+TGG | + | Chr3:65878223-65878242 | MsG0380015146.01.T01:intron | 35.0% | |
| CAAGTATTTGTGAAGAAGCC+TGG | + | Chr3:65878886-65878905 | MsG0380015146.01.T01:CDS | 40.0% | |
| GAATTGATGTTTGGACAGGT+TGG | + | Chr3:65878939-65878958 | MsG0380015146.01.T01:three_prime_UTR | 40.0% | |
| TGGTGTATCATGTGTGAACA+CGG | + | Chr3:65878531-65878550 | MsG0380015146.01.T01:intron | 40.0% | |
| TTCTCAAATTAGGTGTGTCC+CGG | - | Chr3:65878675-65878694 | None:intergenic | 40.0% | |
| !!! | ACAATCAGCATGTTGGCTTT+TGG | + | Chr3:65878511-65878530 | MsG0380015146.01.T01:intron | 40.0% |
| CAGAGAGAAGAAGACTAGAC+TGG | + | Chr3:65877817-65877836 | MsG0380015146.01.T01:five_prime_UTR | 45.0% | |
| GCACGCACACTTCTCAAATT+AGG | - | Chr3:65878685-65878704 | None:intergenic | 45.0% | |
| GTATTTGTGAAGAAGCCTGG+TGG | + | Chr3:65878889-65878908 | MsG0380015146.01.T01:CDS | 45.0% | |
| TGCAGACATCAGACACGAAA+CGG | + | Chr3:65878561-65878580 | MsG0380015146.01.T01:intron | 45.0% | |
| ! | CTTTAAAAGGGCCAAAGCTC+TGG | - | Chr3:65878919-65878938 | None:intergenic | 45.0% |
| ! | GGAACGAGTCGTTATTGACT+CGG | + | Chr3:65877898-65877917 | MsG0380015146.01.T01:CDS | 45.0% |
| !! | CAGAGCTTTGGCCCTTTTAA+AGG | + | Chr3:65878917-65878936 | MsG0380015146.01.T01:CDS | 45.0% |
| !!! | TTATTTTATCAAATTATTAT+TGG | - | Chr3:65878604-65878623 | None:intergenic | 5.0% |
| AAGACAAACAGAGGAGGGAG+CGG | - | Chr3:65878793-65878812 | None:intergenic | 50.0% | |
| AATTAGGTGTGTCCCGGTGT+CGG | - | Chr3:65878669-65878688 | None:intergenic | 50.0% | |
| AGAGAACAGCGATGGCTAGA+CGG | + | Chr3:65877862-65877881 | MsG0380015146.01.T01:exon | 50.0% | |
| AGGTTGGATGTCATCCATGG+AGG | + | Chr3:65878955-65878974 | MsG0380015146.01.T01:three_prime_UTR | 50.0% | |
| ATCATGTGTGAACACGGACG+CGG | + | Chr3:65878537-65878556 | MsG0380015146.01.T01:intron | 50.0% | |
| CATCAGACACGAAACGGACA+TGG | + | Chr3:65878567-65878586 | MsG0380015146.01.T01:intron | 50.0% | |
| GCGTGCTTAGAATACCTCCA+TGG | - | Chr3:65878972-65878991 | None:intergenic | 50.0% | |
| TTTGTGAAGAAGCCTGGTGG+TGG | + | Chr3:65878892-65878911 | MsG0380015146.01.T01:CDS | 50.0% | |
| ! | GACAGGTTGGATGTCATCCA+TGG | + | Chr3:65878952-65878971 | MsG0380015146.01.T01:three_prime_UTR | 50.0% |
| !! | CATATGTGTCGGTGTCGTGT+TGG | + | Chr3:65878640-65878659 | MsG0380015146.01.T01:intron | 50.0% |
| AGACAAACAGAGGAGGGAGC+GGG | - | Chr3:65878792-65878811 | None:intergenic | 55.0% | |
| GAGGTGAGAGAGAACAGCGA+TGG | + | Chr3:65877854-65877873 | MsG0380015146.01.T01:exon | 55.0% | |
| ACAAACAGAGGAGGGAGCGG+GGG | - | Chr3:65878790-65878809 | None:intergenic | 60.0% | |
| CTAGACGGCGAGTCGAGACT+CGG | + | Chr3:65877877-65877896 | MsG0380015146.01.T01:CDS | 60.0% | |
| GACAAACAGAGGAGGGAGCG+GGG | - | Chr3:65878791-65878810 | None:intergenic | 60.0% | |
| ! | CAAAGCTCTGGTCCACCACC+AGG | - | Chr3:65878907-65878926 | None:intergenic | 60.0% |
| ! | GTCGTGTTGGTGTCCGACAC+CGG | + | Chr3:65878653-65878672 | MsG0380015146.01.T01:intron | 60.0% |
| ! | TCGTGTTGGTGTCCGACACC+GGG | + | Chr3:65878654-65878673 | MsG0380015146.01.T01:intron | 60.0% |
| !! | CTGGTGGTGGACCAGAGCTT+TGG | + | Chr3:65878905-65878924 | MsG0380015146.01.T01:CDS | 60.0% |
| AACAGAGGAGGGAGCGGGGG+TGG | - | Chr3:65878787-65878806 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 65877813 | 65879205 | 65877813 | ID=MsG0380015146.01;Name=MsG0380015146.01 |
| Chr3 | mRNA | 65877813 | 65879205 | 65877813 | ID=MsG0380015146.01.T01;Parent=MsG0380015146.01;Name=MsG0380015146.01.T01;_AED=0.50;_eAED=0.50;_QI=60|1|1|1|0|0|2|269|71 |
| Chr3 | exon | 65877813 | 65878016 | 65877813 | ID=MsG0380015146.01.T01:exon:22840;Parent=MsG0380015146.01.T01 |
| Chr3 | exon | 65878865 | 65879205 | 65878865 | ID=MsG0380015146.01.T01:exon:22841;Parent=MsG0380015146.01.T01 |
| Chr3 | five_prime_UTR | 65877813 | 65877872 | 65877813 | ID=MsG0380015146.01.T01:five_prime_utr;Parent=MsG0380015146.01.T01 |
| Chr3 | CDS | 65877873 | 65878016 | 65877873 | ID=MsG0380015146.01.T01:cds;Parent=MsG0380015146.01.T01 |
| Chr3 | CDS | 65878865 | 65878936 | 65878865 | ID=MsG0380015146.01.T01:cds;Parent=MsG0380015146.01.T01 |
| Chr3 | three_prime_UTR | 65878937 | 65879205 | 65878937 | ID=MsG0380015146.01.T01:three_prime_utr;Parent=MsG0380015146.01.T01 |
| Gene Sequence |
| Protein sequence |
