AlfalfaGEDB | MsG0580024051.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024051.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047791.01	MsG0580024051.01	0.844533	7.126308e-59	1.153966e-55
MsG0180000205.01	MsG0580024051.01	0.813404	2.604042e-51	1.699043e-48
MsG0180000384.01	MsG0580024051.01	0.800480	1.413354e-48	6.587776e-46
MsG0180000614.01	MsG0580024051.01	0.859199	4.877673e-63	1.277695e-59
MsG0180000615.01	MsG0580024051.01	0.816672	4.900037e-52	3.494404e-49
MsG0180000743.01	MsG0580024051.01	0.827405	1.593402e-54	1.536960e-51
MsG0180000982.01	MsG0580024051.01	0.822346	2.488323e-53	2.077346e-50
MsG0180001886.01	MsG0580024051.01	0.837408	5.291054e-57	6.866044e-54
MsG0180002458.01	MsG0580024051.01	0.800521	1.386212e-48	6.468154e-46
MsG0180002489.01	MsG0580024051.01	0.819783	9.690375e-53	7.529420e-50
MsG0180002491.01	MsG0580024051.01	0.802069	6.681278e-49	3.242254e-46
MsG0180002547.01	MsG0580024051.01	0.812995	3.201176e-51	2.065543e-48
MsG0180002686.01	MsG0580024051.01	-0.801160	1.026278e-48	4.867068e-46
MsG0180003124.01	MsG0580024051.01	0.838047	3.626759e-57	4.800397e-54
MsG0180003793.01	MsG0580024051.01	0.831555	1.562387e-55	1.701493e-52
MsG0180004050.01	MsG0580024051.01	0.811207	7.855680e-51	4.833314e-48
MsG0180004451.01	MsG0580024051.01	-0.814183	1.754236e-51	1.168975e-48
MsG0180004481.01	MsG0580024051.01	0.825075	5.713133e-54	5.154108e-51
MsG0180004482.01	MsG0580024051.01	0.810910	9.112641e-51	5.562935e-48
MsG0180004509.01	MsG0580024051.01	0.800201	1.610842e-48	7.455207e-46
MsG0180004854.01	MsG0580024051.01	0.849273	3.594572e-60	6.778553e-57
MsG0180004861.01	MsG0580024051.01	0.827936	1.187680e-54	1.163359e-51
MsG0180005334.01	MsG0580024051.01	0.833737	4.489773e-56	5.217032e-53
MsG0180005993.01	MsG0580024051.01	0.808828	2.556787e-50	1.476850e-47
MsG0180006013.01	MsG0580024051.01	0.810844	9.418177e-51	5.739162e-48
MsG0280006411.01	MsG0580024051.01	0.830990	2.150640e-55	2.303073e-52
MsG0280006422.01	MsG0580024051.01	0.872364	3.365854e-67	1.404778e-63
MsG0280010776.01	MsG0580024051.01	0.826296	2.932662e-54	2.739173e-51
MsG0280011388.01	MsG0580024051.01	0.822950	1.800703e-53	1.529248e-50
MsG0380012073.01	MsG0580024051.01	0.814325	1.632219e-51	1.091846e-48
MsG0380012566.01	MsG0580024051.01	0.831975	1.230693e-55	1.357023e-52
MsG0380012661.01	MsG0580024051.01	-0.804830	1.789508e-49	9.321055e-47
MsG0380014647.01	MsG0580024051.01	0.846050	2.770240e-59	4.708848e-56
MsG0480018477.01	MsG0580024051.01	0.835874	1.301678e-56	1.612690e-53
MsG0480018906.01	MsG0580024051.01	0.815179	1.055281e-51	7.223767e-49
MsG0480021448.01	MsG0580024051.01	0.888537	5.406230e-73	4.205297e-69
MsG0480021451.01	MsG0580024051.01	0.849006	4.266176e-60	7.975888e-57
MsG0480021453.01	MsG0580024051.01	0.884979	1.206582e-71	8.133946e-68
MsG0480021464.01	MsG0580024051.01	0.803781	2.959167e-49	1.500217e-46
MsG0480021706.01	MsG0580024051.01	0.824285	8.770749e-54	7.734181e-51
MsG0480021750.01	MsG0580024051.01	0.826108	3.250369e-54	3.019767e-51
MsG0480021994.01	MsG0580024051.01	0.827260	1.725458e-54	1.657296e-51
MsG0480022052.01	MsG0580024051.01	-0.812329	4.479053e-51	2.839625e-48
MsG0480022164.01	MsG0580024051.01	0.806595	7.622305e-50	4.153630e-47
MsG0480022223.01	MsG0580024051.01	0.866017	3.876947e-65	1.287475e-61
MsG0480022696.01	MsG0580024051.01	0.833641	4.744638e-56	5.497103e-53
MsG0480022768.01	MsG0580024051.01	0.800672	1.291540e-48	6.049572e-46
MsG0480022960.01	MsG0580024051.01	0.802615	5.157256e-49	2.537721e-46
MsG0480023356.01	MsG0580024051.01	0.829273	5.645531e-55	5.751078e-52
MsG0480023357.01	MsG0580024051.01	0.800011	1.760269e-48	8.107914e-46
MsG0480023814.01	MsG0580024051.01	0.820166	7.916984e-53	6.217656e-50
MsG0580024051.01	MsG0580024619.01	0.828262	9.912122e-55	9.801503e-52
MsG0580024051.01	MsG0580025268.01	0.843808	1.115879e-58	1.766203e-55
MsG0580024051.01	MsG0580025756.01	0.864581	1.096951e-64	3.461769e-61
MsG0580024051.01	MsG0580026301.01	0.842329	2.763810e-58	4.176339e-55
MsG0580024051.01	MsG0580026485.01	0.828107	1.080344e-54	1.063581e-51
MsG0580024051.01	MsG0580026718.01	0.836696	8.044165e-57	1.021636e-53
MsG0580024051.01	MsG0580026777.01	0.810250	1.265810e-50	7.592475e-48
MsG0580024051.01	MsG0580029717.01	0.810832	9.471845e-51	5.770118e-48
MsG0580024051.01	MsG0580029924.01	0.803089	4.116058e-49	2.049860e-46
MsG0580024051.01	MsG0680030350.01	0.811220	7.808442e-51	4.805702e-48
MsG0580024051.01	MsG0680030415.01	0.813367	2.653649e-51	1.729644e-48
MsG0580024051.01	MsG0680031507.01	0.801331	9.471179e-49	4.510852e-46
MsG0580024051.01	MsG0680031628.01	0.857521	1.542668e-62	3.818337e-59
MsG0580024051.01	MsG0680031646.01	0.801733	7.831472e-49	3.767948e-46
MsG0580024051.01	MsG0680034671.01	0.804145	2.485780e-49	1.272076e-46
MsG0580024051.01	MsG0680035765.01	0.807734	4.373128e-50	2.454651e-47
MsG0580024051.01	MsG0680035888.01	0.800839	1.193968e-48	5.616393e-46
MsG0580024051.01	MsG0780037124.01	0.816834	4.508624e-52	3.229525e-49
MsG0580024051.01	MsG0780037240.01	0.828950	6.759426e-55	6.819123e-52
MsG0580024051.01	MsG0780038400.01	0.846349	2.297437e-59	3.942908e-56
MsG0580024051.01	MsG0780040162.01	0.828049	1.115892e-54	1.096669e-51
MsG0580024051.01	MsG0780040545.01	0.833000	6.855508e-56	7.792841e-53
MsG0580024051.01	MsG0780040858.01	0.801811	7.548863e-49	3.639312e-46
MsG0580024051.01	MsG0780041223.01	0.851630	7.828624e-61	1.594364e-57
MsG0580024051.01	MsG0780041268.01	0.809711	1.653481e-50	9.778925e-48
MsG0580024051.01	MsG0780041295.01	0.843055	1.772212e-58	2.739155e-55
MsG0580024051.01	MsG0780041392.01	0.817346	3.459403e-52	2.512918e-49
MsG0580024051.01	MsG0780041462.01	0.819678	1.023853e-52	7.931577e-50
MsG0580024051.01	MsG0880042779.01	0.801910	7.203073e-49	3.481373e-46
MsG0580024051.01	MsG0880043465.01	0.810999	8.717026e-51	5.334447e-48
MsG0580024051.01	MsG0880043477.01	0.837405	5.300462e-57	6.877570e-54
MsG0580024051.01	MsG0880044394.01	0.828862	7.099378e-55	7.143358e-52
MsG0580024051.01	MsG0880044975.01	0.831829	1.337294e-55	1.468284e-52
MsG0580024051.01	MsG0880045145.01	0.824278	8.801345e-54	7.759760e-51
MsG0580024051.01	MsG0880045951.01	0.807704	4.438337e-50	2.489231e-47
MsG0580024051.01	MsG0880045965.01	0.836251	1.043919e-56	1.308430e-53
MsG0580024051.01	MsG0880046126.01	0.825249	5.196674e-54	4.711260e-51
MsG0580024051.01	MsG0880046177.01	0.810136	1.339285e-50	8.009253e-48
MsG0580024051.01	MsG0880047427.01	0.824961	6.078436e-54	5.465830e-51
MsG0580024051.01	MsG0880047448.01	0.805317	1.414962e-49	7.462703e-47
MsG0380015027.01	MsG0580024051.01	0.847657	1.006929e-59	1.802406e-56
MsG0380015146.01	MsG0580024051.01	0.826511	2.606077e-54	2.449109e-51
MsG0380015400.01	MsG0580024051.01	0.810748	9.878932e-51	6.004475e-48
MsG0380015466.01	MsG0580024051.01	0.819118	1.373865e-52	1.047880e-49
MsG0380015598.01	MsG0580024051.01	-0.800637	1.312920e-48	6.144205e-46
MsG0380016133.01	MsG0580024051.01	0.824665	7.138249e-54	6.365075e-51
MsG0380016559.01	MsG0580024051.01	0.830306	3.163711e-55	3.320367e-52
MsG0380016560.01	MsG0580024051.01	0.845693	3.464434e-59	5.821592e-56
MsG0380016775.01	MsG0580024051.01	0.809607	1.740789e-50	1.026727e-47
MsG0380016860.01	MsG0580024051.01	0.862900	3.651964e-64	1.086913e-60
MsG0380016886.01	MsG0580024051.01	0.802425	5.646006e-49	2.764875e-46
MsG0380017351.01	MsG0580024051.01	0.814025	1.900670e-51	1.261135e-48
MsG0380017382.01	MsG0580024051.01	0.825728	4.001323e-54	3.677420e-51
MsG0380018033.01	MsG0580024051.01	0.821420	4.075569e-53	3.314164e-50
MsG0380018044.01	MsG0580024051.01	0.811462	6.918289e-51	4.285177e-48
MsG0480018110.01	MsG0580024051.01	0.843662	1.220428e-58	1.923121e-55
MsG0280006483.01	MsG0580024051.01	0.850889	1.267704e-60	2.520171e-57
MsG0280006593.01	MsG0580024051.01	0.802786	4.754828e-49	2.349994e-46
MsG0280006993.01	MsG0580024051.01	0.812124	4.964957e-51	3.130134e-48
MsG0280007847.01	MsG0580024051.01	0.829286	5.603469e-55	5.710694e-52
MsG0280008417.01	MsG0580024051.01	0.802286	6.029283e-49	2.942149e-46
MsG0280010177.01	MsG0580024051.01	0.807190	5.705810e-50	3.157603e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 15 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTCATTCATTTCTGTTTA+TGG	0.018661	5:-299572	MsG0580024051.01.T01:intron
TTCTCTATGGGTTCTTCATA+AGG	0.314427	5:-299602	MsG0580024051.01.T01:intron
GTACCTACACTCATTCTCTA+TGG	0.327232	5:-299615	MsG0580024051.01.T01:CDS
ATAATAACCTCTTGAATCCT+TGG	0.405339	5:+299720	None:intergenic
AGGTACGTTGAAAGAGATCA+TGG	0.523995	5:+299632	None:intergenic
AAAGAGATCATGGAGAGTAG+TGG	0.552251	5:+299642	None:intergenic
GAGTGATTAGATGGAGAAAG+CGG	0.556550	5:-299781	None:intergenic
TACCTACACTCATTCTCTAT+GGG	0.556578	5:-299614	MsG0580024051.01.T01:CDS
GACTTGTGCGGCGTTTACCA+AGG	0.605250	5:-299737	MsG0580024051.01.T01:CDS
GCGTTTACCAAGGATTCAAG+AGG	0.609633	5:-299727	MsG0580024051.01.T01:CDS
TGGAGAGTAGTGGAATCAGA+AGG	0.642946	5:+299652	None:intergenic
AATCAGAAGGATCAACCTCA+CGG	0.688438	5:+299665	None:intergenic
GAACTTCGTTAATTACCGTG+AGG	0.698092	5:-299680	MsG0580024051.01.T01:CDS
ACGCAATTCTGAGACTTGTG+CGG	0.704714	5:-299749	MsG0580024051.01.T01:CDS
AACCCATAGAGAATGAGTGT+AGG	0.739379	5:+299612	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATAATAACCTCTTGAATCCT+TGG	+	Chr5:299533-299552	None:intergenic	30.0%
	CACTCATTCATTTCTGTTTA+TGG	-	Chr5:299678-299697	MsG0580024051.01.T01:CDS	30.0%
	TACCTACACTCATTCTCTAT+GGG	-	Chr5:299636-299655	MsG0580024051.01.T01:CDS	35.0%
	TTCTCTATGGGTTCTTCATA+AGG	-	Chr5:299648-299667	MsG0580024051.01.T01:CDS	35.0%
	AAAGAGATCATGGAGAGTAG+TGG	+	Chr5:299611-299630	None:intergenic	40.0%
	AACCCATAGAGAATGAGTGT+AGG	+	Chr5:299641-299660	None:intergenic	40.0%
	AATCAGAAGGATCAACCTCA+CGG	+	Chr5:299588-299607	None:intergenic	40.0%
	AGGTACGTTGAAAGAGATCA+TGG	+	Chr5:299621-299640	None:intergenic	40.0%
	GAACTTCGTTAATTACCGTG+AGG	-	Chr5:299570-299589	MsG0580024051.01.T01:intron	40.0%
	GTACCTACACTCATTCTCTA+TGG	-	Chr5:299635-299654	MsG0580024051.01.T01:CDS	40.0%
	TGCAATGCTACTGCAGTATT+CGG	-	Chr5:299738-299757	MsG0580024051.01.T01:CDS	40.0%
	GCGTTTACCAAGGATTCAAG+AGG	-	Chr5:299523-299542	MsG0580024051.01.T01:intron	45.0%
!	TGGAGAGTAGTGGAATCAGA+AGG	+	Chr5:299601-299620	None:intergenic	45.0%
!!	ACGCAATTCTGAGACTTGTG+CGG	-	Chr5:299501-299520	MsG0580024051.01.T01:intron	45.0%
	ATCTGCCGCTGATAACCTCA+AGG	-	Chr5:299768-299787	MsG0580024051.01.T01:CDS	50.0%
	GACTTGTGCGGCGTTTACCA+AGG	-	Chr5:299513-299532	MsG0580024051.01.T01:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	299479	299793	299479	ID=MsG0580024051.01;Name=MsG0580024051.01
Chr5	mRNA	299479	299793	299479	ID=MsG0580024051.01.T01;Parent=MsG0580024051.01;Name=MsG0580024051.01.T01;_AED=0.31;_eAED=0.83;_QI=0\|0\|0\|1\|0\|0\|2\|0\|76
Chr5	exon	299603	299793	299603	ID=MsG0580024051.01.T01:exon:89;Parent=MsG0580024051.01.T01
Chr5	CDS	299603	299793	299603	ID=MsG0580024051.01.T01:cds;Parent=MsG0580024051.01.T01
Chr5	CDS	299479	299518	299479	ID=MsG0580024051.01.T01:cds;Parent=MsG0580024051.01.T01
Chr5	mRNA	299479	299721	299479	ID=MsG0580024051.01.T02;Parent=MsG0580024051.01;Name=MsG0580024051.01.T02;_AED=0.37;_eAED=1.00;_QI=0\|0\|0\|1\|0\|0\|2\|0\|52
Chr5	exon	299603	299721	299603	ID=MsG0580024051.01.T02:exon:91;Parent=MsG0580024051.01.T02
Chr5	CDS	299603	299721	299603	ID=MsG0580024051.01.T02:cds;Parent=MsG0580024051.01.T02
Chr5	CDS	299479	299518	299479	ID=MsG0580024051.01.T02:cds;Parent=MsG0580024051.01.T02
Chr5	mRNA	299554	299721	299554	ID=MsG0580024051.01.T03;Parent=MsG0580024051.01;Name=MsG0580024051.01.T03;_AED=0.36;_eAED=0.36;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|55
Chr5	exon	299554	299721	299554	ID=MsG0580024051.01.T03:exon:92;Parent=MsG0580024051.01.T03
Chr5	CDS	299554	299721	299554	ID=MsG0580024051.01.T03:cds;Parent=MsG0580024051.01.T03

Gene Sequence

>MsG0580024051.01.T01

ATGGAGAAAGCGGTGAGAGCATACGCAATTCTGAGACTTGTGCGGCGTTTACCAAGGATTCAAGAGGTTATTATGCTAAGTACGCTCGCGAGAACTTCGTTAATTACCGTGAGGTTGATCCTTCTGATTCCACTACTCTCCATGATCTCTTTCAACGTACCTACACTCATTCTCTATGGGTTCTTCATAAGTATTCGGTCGATGAATCTGCCGCTGATAACCTCAAGGTGA

>MsG0580024051.01.T03

ATGCTAAGTACGCTCGCGAGAACTTCGTTAATTACCGTGAGGTTGATCCTTCTGATTCCACTACTCTCCATGATCTCTTTCAACGTACCTACACTCATTCTCTATGGGTTCTTCATAAGGTTCGTTTCACTCATTCATTTCTGTTTATGGATCTTAATTGATGATTGA

>MsG0580024051.01.T02

ATGCTAAGTACGCTCGCGAGAACTTCGTTAATTACCGTGAGGTTGATCCTTCTGATTCCACTACTCTCCATGATCTCTTTCAACGTACCTACACTCATTCTCTATGGGTTCTTCATAAGTATTCGGTCGATGAATCTGCCGCTGATAACCTCAAGGTGA

Protein sequence

>MsG0580024051.01.T01

MEKAVRAYAILRLVRRLPRIQEVIMLSTLARTSLITVRLILLIPLLSMISFNVPTLILYGFFISIRSMNLPLITSR*

>MsG0580024051.01.T03

MLSTLARTSLITVRLILLIPLLSMISFNVPTLILYGFFIRFVSLIHFCLWILIDD*

>MsG0580024051.01.T02

MLSTLARTSLITVRLILLIPLLSMISFNVPTLILYGFFISIRSMNLPLITSR*