Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028284.01.T01 | XP_003615340.1 | 93.407 | 91 | 6 | 0 | 1 | 91 | 1 | 91 | 1.13E-50 | 164 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028284.01.T01 | G7JYH8 | 93.407 | 91 | 6 | 0 | 1 | 91 | 1 | 91 | 5.39e-51 | 164 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049121.01 | MsG0580028284.01 | 0.843714 | 1.182526e-58 | 1.866230e-55 |
| MsG0180000271.01 | MsG0580028284.01 | 0.813183 | 2.911481e-51 | 1.888052e-48 |
| MsG0180000284.01 | MsG0580028284.01 | 0.850525 | 1.605601e-60 | 3.153732e-57 |
| MsG0180000503.01 | MsG0580028284.01 | 0.811946 | 5.428104e-51 | 3.405596e-48 |
| MsG0180000982.01 | MsG0580028284.01 | 0.823569 | 1.290890e-53 | 1.115592e-50 |
| MsG0180001522.01 | MsG0580028284.01 | 0.801956 | 7.050650e-49 | 3.411493e-46 |
| MsG0180002283.01 | MsG0580028284.01 | 0.807818 | 4.198236e-50 | 2.361537e-47 |
| MsG0180002489.01 | MsG0580028284.01 | 0.870188 | 1.762521e-66 | 6.794694e-63 |
| MsG0180002491.01 | MsG0580028284.01 | 0.831297 | 1.807580e-55 | 1.953288e-52 |
| MsG0180003088.01 | MsG0580028284.01 | 0.808085 | 3.683143e-50 | 2.086444e-47 |
| MsG0180003864.01 | MsG0580028284.01 | 0.850575 | 1.553597e-60 | 3.056797e-57 |
| MsG0180004599.01 | MsG0580028284.01 | 0.820445 | 6.832862e-53 | 5.407671e-50 |
| MsG0180004996.01 | MsG0580028284.01 | 0.830246 | 3.271078e-55 | 3.427457e-52 |
| MsG0180005398.01 | MsG0580028284.01 | 0.804554 | 2.043303e-49 | 1.056668e-46 |
| MsG0180005842.01 | MsG0580028284.01 | 0.807006 | 6.240532e-50 | 3.437037e-47 |
| MsG0180006013.01 | MsG0580028284.01 | 0.816469 | 5.443709e-52 | 3.860403e-49 |
| MsG0180006016.01 | MsG0580028284.01 | 0.844822 | 5.956997e-59 | 9.734632e-56 |
| MsG0180006069.01 | MsG0580028284.01 | 0.803390 | 3.566019e-49 | 1.789784e-46 |
| MsG0180006138.01 | MsG0580028284.01 | 0.819856 | 9.321282e-53 | 7.257383e-50 |
| MsG0280006356.01 | MsG0580028284.01 | 0.801728 | 7.853015e-49 | 3.777762e-46 |
| MsG0280006411.01 | MsG0580028284.01 | 0.831011 | 2.125266e-55 | 2.277328e-52 |
| MsG0280010702.01 | MsG0580028284.01 | 0.801093 | 1.059255e-48 | 5.015196e-46 |
| MsG0280010901.01 | MsG0580028284.01 | 0.801246 | 9.854962e-49 | 4.683780e-46 |
| MsG0280011235.01 | MsG0580028284.01 | 0.815445 | 9.211360e-52 | 6.352159e-49 |
| MsG0280011446.01 | MsG0580028284.01 | 0.805615 | 1.225245e-49 | 6.511800e-47 |
| MsG0380011537.01 | MsG0580028284.01 | 0.822415 | 2.397952e-53 | 2.005851e-50 |
| MsG0380012650.01 | MsG0580028284.01 | 0.810033 | 1.409191e-50 | 8.405229e-48 |
| MsG0380012930.01 | MsG0580028284.01 | 0.814362 | 1.601082e-51 | 1.072101e-48 |
| MsG0380014330.01 | MsG0580028284.01 | 0.816115 | 6.530845e-52 | 4.587051e-49 |
| MsG0380014365.01 | MsG0580028284.01 | 0.831043 | 2.087572e-55 | 2.238945e-52 |
| MsG0380014561.01 | MsG0580028284.01 | 0.814180 | 1.756804e-51 | 1.170583e-48 |
| MsG0380014770.01 | MsG0580028284.01 | 0.807525 | 4.843970e-50 | 2.704167e-47 |
| MsG0380014885.01 | MsG0580028284.01 | 0.814009 | 1.916244e-51 | 1.270915e-48 |
| MsG0480018473.01 | MsG0580028284.01 | 0.821492 | 3.924309e-53 | 3.197354e-50 |
| MsG0480018906.01 | MsG0580028284.01 | 0.809658 | 1.697069e-50 | 1.002291e-47 |
| MsG0480019434.01 | MsG0580028284.01 | 0.804990 | 1.656819e-49 | 8.665194e-47 |
| MsG0480020065.01 | MsG0580028284.01 | 0.833811 | 4.302954e-56 | 5.011464e-53 |
| MsG0480020090.01 | MsG0580028284.01 | 0.844102 | 9.307557e-59 | 1.486967e-55 |
| MsG0480020372.01 | MsG0580028284.01 | 0.803185 | 3.932445e-49 | 1.963365e-46 |
| MsG0480020416.01 | MsG0580028284.01 | 0.832064 | 1.170066e-55 | 1.293463e-52 |
| MsG0480021240.01 | MsG0580028284.01 | 0.803973 | 2.699543e-49 | 1.375397e-46 |
| MsG0480021448.01 | MsG0580028284.01 | 0.820857 | 5.494047e-53 | 4.398387e-50 |
| MsG0480021453.01 | MsG0580028284.01 | 0.801718 | 7.890697e-49 | 3.794934e-46 |
| MsG0480021464.01 | MsG0580028284.01 | 0.865971 | 4.009547e-65 | 1.329380e-61 |
| MsG0480021695.01 | MsG0580028284.01 | 0.815456 | 9.157567e-52 | 6.317132e-49 |
| MsG0480021994.01 | MsG0580028284.01 | 0.804978 | 1.665936e-49 | 8.710556e-47 |
| MsG0480022052.01 | MsG0580028284.01 | -0.809950 | 1.468437e-50 | 8.739297e-48 |
| MsG0480022183.01 | MsG0580028284.01 | 0.813359 | 2.663551e-51 | 1.735765e-48 |
| MsG0480022223.01 | MsG0580028284.01 | 0.804029 | 2.628484e-49 | 1.341100e-46 |
| MsG0480022481.01 | MsG0580028284.01 | 0.812316 | 4.508195e-51 | 2.857063e-48 |
| MsG0480022752.01 | MsG0580028284.01 | 0.814970 | 1.174490e-51 | 7.994523e-49 |
| MsG0480023014.01 | MsG0580028284.01 | 0.811309 | 7.467085e-51 | 4.606196e-48 |
| MsG0480023025.01 | MsG0580028284.01 | 0.800640 | 1.310935e-48 | 6.135453e-46 |
| MsG0480023088.01 | MsG0580028284.01 | 0.837857 | 4.057405e-57 | 5.339372e-54 |
| MsG0480023994.01 | MsG0580028284.01 | 0.847228 | 1.320202e-59 | 2.330843e-56 |
| MsG0480024014.01 | MsG0580028284.01 | 0.800732 | 1.255227e-48 | 5.888415e-46 |
| MsG0580025134.01 | MsG0580028284.01 | 0.839648 | 1.397457e-57 | 1.942265e-54 |
| MsG0580025489.01 | MsG0580028284.01 | 0.806734 | 7.124948e-50 | 3.896644e-47 |
| MsG0580025520.01 | MsG0580028284.01 | 0.800385 | 1.477401e-48 | 6.870076e-46 |
| MsG0580025756.01 | MsG0580028284.01 | 0.801720 | 7.881260e-49 | 3.790612e-46 |
| MsG0580026047.01 | MsG0580028284.01 | 0.843301 | 1.523889e-58 | 2.374187e-55 |
| MsG0380015146.01 | MsG0580028284.01 | 0.804267 | 2.345112e-49 | 1.203803e-46 |
| MsG0380015157.01 | MsG0580028284.01 | 0.829010 | 6.537680e-55 | 6.607028e-52 |
| MsG0380016055.01 | MsG0580028284.01 | 0.819849 | 9.354990e-53 | 7.282102e-50 |
| MsG0380016133.01 | MsG0580028284.01 | 0.812586 | 3.936070e-51 | 2.512407e-48 |
| MsG0380016581.01 | MsG0580028284.01 | 0.849829 | 2.514997e-60 | 4.828914e-57 |
| MsG0380016624.01 | MsG0580028284.01 | 0.839299 | 1.721966e-57 | 2.367804e-54 |
| MsG0380017039.01 | MsG0580028284.01 | 0.822191 | 2.703145e-53 | 2.246464e-50 |
| MsG0380017226.01 | MsG0580028284.01 | 0.814130 | 1.801998e-51 | 1.199056e-48 |
| MsG0380017403.01 | MsG0580028284.01 | 0.829265 | 5.670521e-55 | 5.775020e-52 |
| MsG0580026340.01 | MsG0580028284.01 | 0.803145 | 4.009114e-49 | 1.999503e-46 |
| MsG0580027074.01 | MsG0580028284.01 | 0.811220 | 7.806293e-51 | 4.804506e-48 |
| MsG0580027228.01 | MsG0580028284.01 | 0.838083 | 3.549925e-57 | 4.703150e-54 |
| MsG0580027605.01 | MsG0580028284.01 | 0.813373 | 2.644645e-51 | 1.724087e-48 |
| MsG0580028284.01 | MsG0580028289.01 | 0.911485 | 5.767407e-83 | 1.238343e-78 |
| MsG0580028284.01 | MsG0680030415.01 | 0.804795 | 1.819836e-49 | 9.470310e-47 |
| MsG0580028284.01 | MsG0680030463.01 | 0.843480 | 1.365487e-58 | 2.139236e-55 |
| MsG0580028284.01 | MsG0680030839.01 | 0.844216 | 8.673527e-59 | 1.390602e-55 |
| MsG0580028284.01 | MsG0680032430.01 | 0.805577 | 1.247944e-49 | 6.625897e-47 |
| MsG0580028284.01 | MsG0680034037.01 | 0.802065 | 6.696430e-49 | 3.249167e-46 |
| MsG0580028284.01 | MsG0680034219.01 | 0.824291 | 8.742009e-54 | 7.710026e-51 |
| MsG0580028284.01 | MsG0680035777.01 | 0.807538 | 4.814086e-50 | 2.688305e-47 |
| MsG0580028284.01 | MsG0780038636.01 | 0.826800 | 2.223752e-54 | 2.107122e-51 |
| MsG0580028284.01 | MsG0780038911.01 | 0.848238 | 6.962624e-60 | 1.269439e-56 |
| MsG0580028284.01 | MsG0780039143.01 | 0.811409 | 7.102699e-51 | 4.393404e-48 |
| MsG0580028284.01 | MsG0780039144.01 | 0.816676 | 4.891316e-52 | 3.488436e-49 |
| MsG0580028284.01 | MsG0780039452.01 | 0.812054 | 5.141889e-51 | 3.235403e-48 |
| MsG0580028284.01 | MsG0780039570.01 | 0.818375 | 2.025200e-52 | 1.513445e-49 |
| MsG0580028284.01 | MsG0780040545.01 | 0.810157 | 1.325535e-50 | 7.931248e-48 |
| MsG0580028284.01 | MsG0780040929.01 | 0.802860 | 4.591125e-49 | 2.273282e-46 |
| MsG0580028284.01 | MsG0780041065.01 | 0.806531 | 7.862421e-50 | 4.277619e-47 |
| MsG0580028284.01 | MsG0780041357.01 | 0.808791 | 2.603837e-50 | 1.502545e-47 |
| MsG0580028284.01 | MsG0780041754.01 | 0.804910 | 1.721687e-49 | 8.986745e-47 |
| MsG0580028284.01 | MsG0880041969.01 | 0.832569 | 8.772345e-56 | 9.843430e-53 |
| MsG0580028284.01 | MsG0880042055.01 | 0.846653 | 1.897927e-59 | 3.289749e-56 |
| MsG0580028284.01 | MsG0880042811.01 | 0.812410 | 4.300676e-51 | 2.732528e-48 |
| MsG0580028284.01 | MsG0880042972.01 | 0.801097 | 1.057362e-48 | 5.006651e-46 |
| MsG0580028284.01 | MsG0880043082.01 | 0.848086 | 7.669006e-60 | 1.391191e-56 |
| MsG0580028284.01 | MsG0880043465.01 | 0.844887 | 5.721885e-59 | 9.368703e-56 |
| MsG0580028284.01 | MsG0880044062.01 | -0.812022 | 5.225066e-51 | 3.284961e-48 |
| MsG0580028284.01 | MsG0880044394.01 | 0.801481 | 8.824237e-49 | 4.218748e-46 |
| MsG0580028284.01 | MsG0880045290.01 | 0.813088 | 3.054591e-51 | 1.975780e-48 |
| MsG0580028284.01 | MsG0880045653.01 | 0.824313 | 8.634814e-54 | 7.620548e-51 |
| MsG0580028284.01 | MsG0880045951.01 | 0.803779 | 2.961731e-49 | 1.501444e-46 |
| MsG0580028284.01 | MsG0880045965.01 | 0.800827 | 1.200429e-48 | 5.644926e-46 |
| MsG0580028284.01 | MsG0880046047.01 | 0.814662 | 1.374161e-51 | 9.276331e-49 |
| MsG0580028284.01 | MsG0880046058.01 | 0.828594 | 8.242953e-55 | 8.230187e-52 |
| MsG0580028284.01 | MsG0880046317.01 | 0.800826 | 1.201144e-48 | 5.648088e-46 |
| MsG0580028284.01 | MsG0880047191.01 | 0.831145 | 1.970611e-55 | 2.119904e-52 |
| MsG0580028284.01 | MsG0880047391.01 | 0.840586 | 7.955790e-58 | 1.138410e-54 |
| MsG0580028284.01 | MsG0880047590.01 | 0.824191 | 9.228237e-54 | 8.116554e-51 |
| MsG0580028284.01 | MsG0880047607.01 | 0.807982 | 3.873044e-50 | 2.188056e-47 |
| MsG0580028284.01 | MsG0880047689.01 | -0.807671 | 4.511095e-50 | 2.527795e-47 |
| MsG0580028284.01 | MsG0880047742.01 | 0.835720 | 1.423889e-56 | 1.756013e-53 |
| MsG0280006595.01 | MsG0580028284.01 | 0.828832 | 7.220665e-55 | 7.259165e-52 |
| MsG0280007161.01 | MsG0580028284.01 | 0.813768 | 2.165060e-51 | 1.426553e-48 |
| MsG0280007311.01 | MsG0580028284.01 | 0.805824 | 1.107722e-49 | 5.918963e-47 |
| MsG0280007636.01 | MsG0580028284.01 | 0.805750 | 1.148260e-49 | 6.124198e-47 |
| MsG0280007815.01 | MsG0580028284.01 | 0.802248 | 6.138564e-49 | 2.992539e-46 |
| MsG0280008286.01 | MsG0580028284.01 | 0.841383 | 4.913275e-58 | 7.207159e-55 |
| MsG0280008417.01 | MsG0580028284.01 | 0.858869 | 6.125742e-63 | 1.586529e-59 |
| MsG0280008556.01 | MsG0580028284.01 | 0.807820 | 4.193034e-50 | 2.358773e-47 |
| MsG0280008907.01 | MsG0580028284.01 | 0.802666 | 5.034398e-49 | 2.480385e-46 |
| MsG0280008970.01 | MsG0580028284.01 | 0.801553 | 8.528681e-49 | 4.084758e-46 |
| MsG0280008971.01 | MsG0580028284.01 | 0.801241 | 9.878514e-49 | 4.694429e-46 |
| MsG0280009024.01 | MsG0580028284.01 | 0.827764 | 1.306249e-54 | 1.273034e-51 |
| MsG0280009140.01 | MsG0580028284.01 | 0.803398 | 3.553116e-49 | 1.783717e-46 |
| MsG0280010389.01 | MsG0580028284.01 | 0.831974 | 1.231478e-55 | 1.357836e-52 |
| MsG0280010416.01 | MsG0580028284.01 | 0.813911 | 2.013502e-51 | 1.331836e-48 |
| MsG0280010531.01 | MsG0580028284.01 | 0.815389 | 9.478051e-52 | 6.525894e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028284.01.T01 | MTR_5g066760 | 93.407 | 91 | 6 | 0 | 1 | 91 | 1 | 91 | 1.37e-54 | 164 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028284.01.T01 | AT2G38465 | 57.971 | 69 | 29 | 0 | 19 | 87 | 13 | 81 | 8.98e-22 | 81.6 |
Find 11 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCACAACAAAACTTCTTT+TGG | 0.285814 | 5:+77825308 | MsG0580028284.01.T01:CDS |
| CATTAATTTAATTAACTATG+AGG | 0.415967 | 5:-77825640 | None:intergenic |
| CTGCTGAACATATCAAGAAT+AGG | 0.429170 | 5:+77825608 | MsG0580028284.01.T01:CDS |
| AAAAGCCTTCTTTGTGGGTC+AGG | 0.435149 | 5:+77825394 | MsG0580028284.01.T01:CDS |
| TATGCAAAAGCCTTCTTTGT+GGG | 0.462187 | 5:+77825389 | MsG0580028284.01.T01:CDS |
| AAAAGTAGCTATGGAAGAGA+TGG | 0.471774 | 5:+77825259 | None:intergenic |
| ATATGCAAAAGCCTTCTTTG+TGG | 0.519860 | 5:+77825388 | MsG0580028284.01.T01:CDS |
| AATTACCTGACCCACAAAGA+AGG | 0.535666 | 5:-77825399 | None:intergenic |
| AAAGTAGCTATGGAAGAGAT+GGG | 0.580832 | 5:+77825260 | None:intergenic |
| GGCTTCAAAGAAACAAGTTG+AGG | 0.622095 | 5:+77825576 | MsG0580028284.01.T01:CDS |
| AGCAAGTGAAGCTGAACTTG+AGG | 0.634101 | 5:+77825555 | MsG0580028284.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTATTTTGAAATTGATTG+AGG | + | Chr5:77825477-77825496 | MsG0580028284.01.T01:intron | 20.0% |
| ! | AGATAAGTAGTAGCTAAAAA+TGG | - | Chr5:77825448-77825467 | None:intergenic | 25.0% |
| ! | ATTGTGGTGTGAAAAAAAAT+AGG | + | Chr5:77825501-77825520 | MsG0580028284.01.T01:intron | 25.0% |
| ! | TATAACTGCAAAAAATGAGA+AGG | + | Chr5:77825531-77825550 | MsG0580028284.01.T01:CDS | 25.0% |
| !! | AATCACAACAAAACTTCTTT+TGG | + | Chr5:77825308-77825327 | MsG0580028284.01.T01:CDS | 25.0% |
| !!! | AAAGAAGTTTTGTTGTGATT+TGG | - | Chr5:77825308-77825327 | None:intergenic | 25.0% |
| AGTAGTAGCTAAAAATGGAA+AGG | - | Chr5:77825443-77825462 | None:intergenic | 30.0% | |
| GTAGTAGCTAAAAATGGAAA+GGG | - | Chr5:77825442-77825461 | None:intergenic | 30.0% | |
| TAGTAGCTAAAAATGGAAAG+GGG | - | Chr5:77825441-77825460 | None:intergenic | 30.0% | |
| TGAAATTGATTGAGGAATTG+TGG | + | Chr5:77825485-77825504 | MsG0580028284.01.T01:intron | 30.0% | |
| ATATGCAAAAGCCTTCTTTG+TGG | + | Chr5:77825388-77825407 | MsG0580028284.01.T01:CDS | 35.0% | |
| CTGCTGAACATATCAAGAAT+AGG | + | Chr5:77825608-77825627 | MsG0580028284.01.T01:CDS | 35.0% | |
| TATGCAAAAGCCTTCTTTGT+GGG | + | Chr5:77825389-77825408 | MsG0580028284.01.T01:CDS | 35.0% | |
| !! | AAATGGAAAGGGGTTGTTTT+TGG | - | Chr5:77825431-77825450 | None:intergenic | 35.0% |
| AATTACCTGACCCACAAAGA+AGG | - | Chr5:77825402-77825421 | None:intergenic | 40.0% | |
| GGCTTCAAAGAAACAAGTTG+AGG | + | Chr5:77825576-77825595 | MsG0580028284.01.T01:CDS | 40.0% | |
| AAAAGCCTTCTTTGTGGGTC+AGG | + | Chr5:77825394-77825413 | MsG0580028284.01.T01:CDS | 45.0% | |
| AGCAAGTGAAGCTGAACTTG+AGG | + | Chr5:77825555-77825574 | MsG0580028284.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 77825269 | 77825651 | 77825269 | ID=MsG0580028284.01;Name=MsG0580028284.01 |
| Chr5 | mRNA | 77825269 | 77825651 | 77825269 | ID=MsG0580028284.01.T01;Parent=MsG0580028284.01;Name=MsG0580028284.01.T01;_AED=0.49;_eAED=0.51;_QI=0|0|0|1|1|1|2|0|91 |
| Chr5 | exon | 77825269 | 77825415 | 77825269 | ID=MsG0580028284.01.T01:exon:46418;Parent=MsG0580028284.01.T01 |
| Chr5 | exon | 77825523 | 77825651 | 77825523 | ID=MsG0580028284.01.T01:exon:46419;Parent=MsG0580028284.01.T01 |
| Chr5 | CDS | 77825269 | 77825415 | 77825269 | ID=MsG0580028284.01.T01:cds;Parent=MsG0580028284.01.T01 |
| Chr5 | CDS | 77825523 | 77825651 | 77825523 | ID=MsG0580028284.01.T01:cds;Parent=MsG0580028284.01.T01 |
| Gene Sequence |
| Protein sequence |
