Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017841.01.T01 | XP_003603741.2 | 94.896 | 529 | 21 | 1 | 1 | 523 | 1 | 529 | 0 | 1005 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000535.01 | MsG0380017841.01 | 0.810808 | 9.587242e-51 | 5.836426e-48 |
| MsG0380016141.01 | MsG0380017841.01 | 0.881388 | 2.501786e-70 | 1.462700e-66 |
| MsG0380016567.01 | MsG0380017841.01 | 0.805293 | 1.431627e-49 | 7.545830e-47 |
| MsG0380017753.01 | MsG0380017841.01 | 0.814083 | 1.845745e-51 | 1.226587e-48 |
| MsG0380017841.01 | MsG0380017913.01 | 0.808867 | 2.507448e-50 | 1.449900e-47 |
| MsG0380017841.01 | MsG0480021300.01 | 0.805399 | 1.360385e-49 | 7.189798e-47 |
| MsG0380017841.01 | MsG0480022761.01 | 0.813114 | 3.014860e-51 | 1.951376e-48 |
| MsG0380017841.01 | MsG0580024773.01 | 0.805275 | 1.443854e-49 | 7.606602e-47 |
| MsG0380017841.01 | MsG0880046432.01 | 0.828544 | 8.476360e-55 | 8.451280e-52 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017841.01.T01 | MTR_3g113720 | 94.896 | 529 | 21 | 1 | 1 | 523 | 1 | 529 | 0.0 | 1005 |
| MsG0380017841.01.T01 | MTR_8g075990 | 59.417 | 515 | 200 | 2 | 14 | 522 | 23 | 534 | 0.0 | 585 |
| MsG0380017841.01.T01 | MTR_5g085730 | 41.614 | 471 | 255 | 10 | 58 | 513 | 32 | 497 | 9.75e-119 | 360 |
| MsG0380017841.01.T01 | MTR_8g099410 | 43.284 | 469 | 246 | 11 | 58 | 512 | 29 | 491 | 6.23e-118 | 357 |
| MsG0380017841.01.T01 | MTR_8g099410 | 43.284 | 469 | 246 | 11 | 58 | 512 | 29 | 491 | 9.41e-117 | 358 |
| MsG0380017841.01.T01 | MTR_4g074960 | 40.000 | 475 | 260 | 8 | 58 | 515 | 29 | 495 | 1.32e-115 | 355 |
| MsG0380017841.01.T01 | MTR_5g093590 | 40.717 | 474 | 254 | 11 | 58 | 512 | 35 | 500 | 1.43e-111 | 341 |
| MsG0380017841.01.T01 | MTR_3g080190 | 40.340 | 471 | 261 | 10 | 58 | 513 | 41 | 506 | 6.64e-111 | 340 |
| MsG0380017841.01.T01 | MTR_5g009970 | 39.744 | 468 | 255 | 7 | 58 | 513 | 42 | 494 | 3.29e-110 | 337 |
| MsG0380017841.01.T01 | MTR_5g010000 | 40.890 | 472 | 252 | 8 | 58 | 517 | 41 | 497 | 1.00e-106 | 328 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017841.01.T01 | AT1G75680 | 65.595 | 529 | 170 | 6 | 1 | 522 | 1 | 524 | 0.0 | 655 |
| MsG0380017841.01.T01 | AT1G19940 | 64.773 | 528 | 167 | 5 | 1 | 522 | 1 | 515 | 0.0 | 652 |
| MsG0380017841.01.T01 | AT1G19940 | 64.773 | 528 | 167 | 5 | 1 | 522 | 1 | 515 | 0.0 | 652 |
| MsG0380017841.01.T01 | AT1G19940 | 65.957 | 423 | 131 | 4 | 1 | 423 | 1 | 410 | 0.0 | 551 |
| MsG0380017841.01.T01 | AT4G11050 | 42.857 | 476 | 252 | 10 | 58 | 519 | 27 | 496 | 1.22e-121 | 368 |
| MsG0380017841.01.T01 | AT4G11050 | 43.067 | 476 | 251 | 11 | 58 | 519 | 27 | 496 | 7.00e-121 | 369 |
| MsG0380017841.01.T01 | AT4G11050 | 43.067 | 476 | 251 | 11 | 58 | 519 | 27 | 496 | 8.55e-121 | 369 |
| MsG0380017841.01.T01 | AT1G64390 | 41.545 | 479 | 260 | 10 | 58 | 522 | 26 | 498 | 4.76e-118 | 361 |
| MsG0380017841.01.T01 | AT2G32990 | 40.466 | 472 | 253 | 11 | 58 | 510 | 40 | 502 | 5.42e-114 | 348 |
| MsG0380017841.01.T01 | AT2G44540 | 41.438 | 473 | 255 | 8 | 52 | 512 | 28 | 490 | 7.90e-111 | 338 |
Find 149 sgRNAs with CRISPR-Local
Find 290 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGAATGTATGATGCCG+GGG | 0.628087 | 3:-101275065 | MsG0380017841.01.T01:CDS |
| AAACTGGTTGCAAGGATGGG+TGG | 0.621140 | 3:-101272599 | MsG0380017841.01.T01:CDS |
| AAATTATAGAAAATCCGCCG+AGG | 0.730261 | 3:-101273681 | MsG0380017841.01.T01:CDS |
| AACAACAAAATAAGTTGGAG+AGG | 0.494998 | 3:-101275133 | MsG0380017841.01.T01:CDS |
| AACAGCACCAACCAACAACT+AGG | 0.593332 | 3:+101276005 | None:intergenic |
| AACCAGCACAAGATTCTCTC+AGG | 0.525412 | 3:-101274943 | MsG0380017841.01.T01:CDS |
| AAGACCACACATTACAGCCT+CGG | 0.582653 | 3:+101273664 | None:intergenic |
| AAGCCCAACCTGGTTCAGTT+GGG | 0.506162 | 3:-101274348 | MsG0380017841.01.T01:CDS |
| AAGGGCCTTTCAAGTTCATA+TGG | 0.416309 | 3:-101273716 | MsG0380017841.01.T01:CDS |
| AAGTGCTCCAGTAGCTACAT+TGG | 0.543749 | 3:+101272548 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACTTGTCTAATACTTCC+AGG | - | Chr3:101275788-101275807 | MsG0380017841.01.T01:five_prime_UTR | 30.0% | |
| AATGCAAAAAACAGAGTTTG+GGG | + | Chr3:101272765-101272784 | None:intergenic | 30.0% | |
| AGAGAAAAATCGAGATCAAA+AGG | - | Chr3:101272811-101272830 | MsG0380017841.01.T01:intron | 30.0% | |
| AGTAAGTAGATGTAACATCT+AGG | + | Chr3:101274716-101274735 | None:intergenic | 30.0% | |
| AGTTAGCAATGTGTTTGTTT+GGG | - | Chr3:101272689-101272708 | MsG0380017841.01.T01:intron | 30.0% | |
| ATTAAATAAGCTCCTTGATC+TGG | + | Chr3:101275526-101275545 | None:intergenic | 30.0% | |
| ATTTCTTGCATTTCACAATG+TGG | - | Chr3:101273062-101273081 | MsG0380017841.01.T01:intron | 30.0% | |
| CAAATGCAAAAAACAGAGTT+TGG | + | Chr3:101272767-101272786 | None:intergenic | 30.0% | |
| GCAGATTTAGATCTAACAAA+AGG | - | Chr3:101273438-101273457 | MsG0380017841.01.T01:intron | 30.0% | |
| GTCTATTCTATGTAATGTGT+AGG | + | Chr3:101274355-101274374 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 101272401 | 101276141 | 101272401 | ID=MsG0380017841.01;Name=MsG0380017841.01 |
| Chr3 | mRNA | 101272401 | 101276141 | 101272401 | ID=MsG0380017841.01.T01;Parent=MsG0380017841.01;Name=MsG0380017841.01.T01;_AED=0.27;_eAED=0.27;_QI=407|0.8|0.66|1|0.8|0.66|6|0|523 |
| Chr3 | exon | 101272401 | 101272702 | 101272401 | ID=MsG0380017841.01.T01:exon:2905;Parent=MsG0380017841.01.T01 |
| Chr3 | exon | 101273111 | 101273290 | 101273111 | ID=MsG0380017841.01.T01:exon:2904;Parent=MsG0380017841.01.T01 |
| Chr3 | exon | 101273624 | 101273771 | 101273624 | ID=MsG0380017841.01.T01:exon:2903;Parent=MsG0380017841.01.T01 |
| Chr3 | exon | 101274325 | 101274762 | 101274325 | ID=MsG0380017841.01.T01:exon:2902;Parent=MsG0380017841.01.T01 |
| Chr3 | exon | 101274886 | 101275169 | 101274886 | ID=MsG0380017841.01.T01:exon:2901;Parent=MsG0380017841.01.T01 |
| Chr3 | exon | 101275515 | 101276141 | 101275515 | ID=MsG0380017841.01.T01:exon:2900;Parent=MsG0380017841.01.T01 |
| Chr3 | five_prime_UTR | 101275735 | 101276141 | 101275735 | ID=MsG0380017841.01.T01:five_prime_utr;Parent=MsG0380017841.01.T01 |
| Chr3 | CDS | 101275515 | 101275734 | 101275515 | ID=MsG0380017841.01.T01:cds;Parent=MsG0380017841.01.T01 |
| Chr3 | CDS | 101274886 | 101275169 | 101274886 | ID=MsG0380017841.01.T01:cds;Parent=MsG0380017841.01.T01 |
| Chr3 | CDS | 101274325 | 101274762 | 101274325 | ID=MsG0380017841.01.T01:cds;Parent=MsG0380017841.01.T01 |
| Chr3 | CDS | 101273624 | 101273771 | 101273624 | ID=MsG0380017841.01.T01:cds;Parent=MsG0380017841.01.T01 |
| Chr3 | CDS | 101273111 | 101273290 | 101273111 | ID=MsG0380017841.01.T01:cds;Parent=MsG0380017841.01.T01 |
| Chr3 | CDS | 101272401 | 101272702 | 101272401 | ID=MsG0380017841.01.T01:cds;Parent=MsG0380017841.01.T01 |
| Gene Sequence |
>MsG0380017841.01.T01 ATGAGAGAAAAATCGAGATCAAAAGGAGGATGTTGTGGTTGGTTTATTGCGTTTGTGATTCTGGCTCTTGTGGTCGGTGCTATTGTTTATGCTGTTAAGAAGAAAATCGATAACTCCAATTCAGATAAACCCGAACCGGTTCCCGGTCCTCCTGGTGCTATTGATCAAAAATATGCTTCTGCTCTCAAAACTGCAATGCAATTCTTCGACGTGCAAAAATCTGGCAAGTTAGAGAACAACAAAATAAGTTGGAGAGGGGATTCGGCTCTTAAGGATGGAAAGCAAGCAGATTTAGATCTAACAAAAGGAATGTATGATGCCGGGGATCACATGAAGTTTGGTTTTCCTATGGCTTTTACTGCCTCGGTGCTATCGTGGGCAATTCTAGAATATGGAGATCAAATGGATGCTGTTGGCCAATTGGAACCAGCACAAGATTCTCTCAGGTGGATCACTGATTTTCTCGTCAATGCTCATCCTTCGGAGAATGTTCTGTATATTCAGGTTGGTGATCCTGTTGCAGATCACAAGTGTTGGAATAGACCTGAGTTAATTACTGAGGAACGACCCCTCTTACAAGTGAACATATCCTGTCCCGGATCAGATGTTGCAGCTGAAACTGCAGCAGCAATGGCATCAGCATCTTTGGTCTTCAAAAAGTCAGATGCTACATATTCAAGCACTCTCCTCAAGCATGCAAAACAGTTATTTACTTTTGCGGACAAGCATAGAGGAATTTACAGTGAGAATATACCTGAAGTTGCGACATATTATAACTCAACTGGATATGGGGATGAGCTTTTGTGGGCAGCTACCTGGCTCTACCATGCTACAGGTGATGATTCCTACCTTCAGTATGTGACTGGCCAAAATGGAGAAGACTATGCTCAATTTGGAAGCCCAACCTGGTTCAGTTGGGACAACAAGCTTGCCGGAACTCAGGTTTTGTTATCTAGAGTAAGCTTCTTCAAGGCAAAGGGCCTTTCAAGTTCATATGGTTCTGGCCTCCAAAATTATAGAAAATCCGCCGAGGCTGTAATGTGTGGTCTTCTTCCAGACTCTCCAACAGCCACAAAAAGCAGAACTGATAATGGTCTGATATGGGTGAGTGAATGGAATGCTTTGCAGCATCCTGTTGCATCTGCATTCTTAGCTTCTGTTTACAGTGACTATATGCTTACAACGCAGACTCCCAATATAAAATGTGATTCAGATTCCTTCAAACCATCAGATCTTCGAGACTTTGCCAGATCTCAGGTATGCATTCATTCATATGAGTTTTCTGGTGGGATATGGAAAGAATTTCCCACAATTTGTGCATCACAGGGAGCTTCAATTCCTGCTAATGCAAAAACTGGTTGCAAGGATGGGTGGCAGTGGCTTGACTCATCCGATCCGAATCCCAATGTAGCTACTGGAGCACTTGTTGGTGGACCTTTCTTAAATGAGACTTACATTGATTCTAGGAACAATTCTATGCAAGGAGAACCAAGCACATATAACAGTGCTGTCATAGTTGGTCTCTTATCAAGTTTAGTCACCACATCTTCGGCCCTTCAATCCTTCTCCTAA |
| Protein sequence |
>MsG0380017841.01.T01 MREKSRSKGGCCGWFIAFVILALVVGAIVYAVKKKIDNSNSDKPEPVPGPPGAIDQKYASALKTAMQFFDVQKSGKLENNKISWRGDSALKDGKQADLDLTKGMYDAGDHMKFGFPMAFTASVLSWAILEYGDQMDAVGQLEPAQDSLRWITDFLVNAHPSENVLYIQVGDPVADHKCWNRPELITEERPLLQVNISCPGSDVAAETAAAMASASLVFKKSDATYSSTLLKHAKQLFTFADKHRGIYSENIPEVATYYNSTGYGDELLWAATWLYHATGDDSYLQYVTGQNGEDYAQFGSPTWFSWDNKLAGTQVLLSRVSFFKAKGLSSSYGSGLQNYRKSAEAVMCGLLPDSPTATKSRTDNGLIWVSEWNALQHPVASAFLASVYSDYMLTTQTPNIKCDSDSFKPSDLRDFARSQVCIHSYEFSGGIWKEFPTICASQGASIPANAKTGCKDGWQWLDSSDPNPNVATGALVGGPFLNETYIDSRNNSMQGEPSTYNSAVIVGLLSSLVTTSSALQSFS* |
