AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024181.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024181.01.T01 MTR_5g006840 100.000 203 0 0 1 203 1 203 6.05e-153 422
MsG0580024181.01.T01 MTR_5g012470 96.000 200 8 0 1 200 1 200 1.32e-145 403
MsG0580024181.01.T01 MTR_2g050000 95.413 109 3 1 95 203 2 108 1.36e-71 213
MsG0580024181.01.T01 MTR_1g112250 30.464 151 94 3 13 155 45 192 8.57e-13 65.5
MsG0580024181.01.T01 MTR_3g109210 26.087 161 117 1 3 163 15 173 1.75e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024181.01.T01 AT3G22630 87.129 202 26 0 1 202 1 202 6.82e-135 376
MsG0580024181.01.T01 AT4G14800 87.374 198 25 0 1 198 1 198 7.28e-132 369
MsG0580024181.01.T01 AT4G14800 80.841 214 25 1 1 198 1 214 2.30e-127 358
MsG0580024181.01.T01 AT3G14290 31.126 151 93 3 13 155 45 192 3.12e-13 66.6

Find 50 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGTTGATAAGTGCATTT+TGG 0.220132 5:+1664412 MsG0580024181.01.T01:CDS
CTTTGTATCAATTCCGTAAT+GGG 0.276520 5:+1661306 MsG0580024181.01.T01:CDS
AAGGGGTGGAATAGTGCTTT+AGG 0.290716 5:+1664793 MsG0580024181.01.T01:three_prime_UTR
TTTGAATGTATTCAGTAAAC+TGG 0.314079 5:-1661274 None:intergenic
AAGCTTGAAAAGGGAGCTTT+TGG 0.328358 5:+1664314 MsG0580024181.01.T01:CDS
AATAGTGCTTTAGGGCTTAT+TGG 0.332443 5:+1664802 MsG0580024181.01.T01:three_prime_UTR
TCTGTGAACATCCTTCTTGC+TGG 0.377484 5:+1664233 MsG0580024181.01.T01:CDS
AGGGGTGGAATAGTGCTTTA+GGG 0.412033 5:+1664794 MsG0580024181.01.T01:three_prime_UTR
GCTTTGTATCAATTCCGTAA+TGG 0.426748 5:+1661305 MsG0580024181.01.T01:CDS
AGAAGGATGTTCACAGAGTA+GGG 0.434546 5:-1664227 None:intergenic
GACACTTCACAAGCTTGAAA+AGG 0.445538 5:+1664304 MsG0580024181.01.T01:CDS
AAACCTTGCATGGTCTCTAA+TGG 0.458990 5:+1664662 MsG0580024181.01.T01:three_prime_UTR
CTAGCGGTGAACCCGGTGAC+AGG 0.462278 5:+1661162 MsG0580024181.01.T01:CDS
AAGAAGGATGTTCACAGAGT+AGG 0.469415 5:-1664228 None:intergenic
GAACATGATCTTGTCTTCGT+TGG 0.478439 5:-1661114 None:intergenic
CTAATTATGTTGTAATCTTG+CGG 0.496093 5:+1664870 MsG0580024181.01.T01:three_prime_UTR
TGGTGAGAGGAATCCCATTA+CGG 0.502878 5:-1661319 None:intergenic
TTTGGAAATCAGATCAAGGT+TGG 0.503786 5:+1664430 MsG0580024181.01.T01:CDS
GGATTAAATCAAGCTGAAGC+AGG 0.515437 5:-1664539 None:intergenic
ACACTTCACAAGCTTGAAAA+GGG 0.519514 5:+1664305 MsG0580024181.01.T01:CDS
AATCAGATCAAGGTTGGTTG+TGG 0.521276 5:+1664436 MsG0580024181.01.T01:CDS
TGGTAATGGCTTCGCGATCG+TGG 0.521317 5:+1661049 MsG0580024181.01.T01:CDS
GCTTGCCACCGCTCTTCGCA+AGG 0.527948 5:+1661373 MsG0580024181.01.T01:CDS
ATGTTGAAATATTAAAGCTC+AGG 0.532504 5:-1664598 None:intergenic
CTTCCACGTTCATTCCGCTA+TGG 0.534157 5:-1664379 None:intergenic
GCTGGTTATGACAAAGAGAC+AGG 0.546617 5:+1664251 MsG0580024181.01.T01:CDS
TAGTCAATGTAATATAGTGA+TGG 0.549687 5:-1664275 None:intergenic
TAATCCGAAAACGCATTCCA+TGG 0.555612 5:-1661024 None:intergenic
GCAGCAGCAGCTGTGGTGAG+AGG 0.566652 5:-1661332 None:intergenic
ATCGCTGCTAGCGGTGAACC+CGG 0.569631 5:+1661155 MsG0580024181.01.T01:CDS
CTTTGTCATAACCAGCAAGA+AGG 0.572175 5:-1664244 None:intergenic
AGTCAATGTAATATAGTGAT+GGG 0.575452 5:-1664274 None:intergenic
AAGAGAGTGAAACCTGTCAC+CGG 0.576291 5:-1661174 None:intergenic
CGACCATTAGAGACCATGCA+AGG 0.579444 5:-1664665 None:intergenic
GAAATTAGCAGCAGCAGCTG+TGG 0.588351 5:-1661339 None:intergenic
GAATACCTTGCGAAGAGCGG+TGG 0.614639 5:-1661378 None:intergenic
ATGTATCTGAAAACCTTGCA+TGG 0.620002 5:+1664652 MsG0580024181.01.T01:three_prime_UTR
TGCATGGCGCGAATCAGTCA+AGG 0.620623 5:+1664511 MsG0580024181.01.T01:CDS
CTTCGTTGGATTTGTGAACG+AGG 0.622483 5:-1661100 None:intergenic
CTTCCATAGCGGAATGAACG+TGG 0.622934 5:+1664376 MsG0580024181.01.T01:CDS
CACAAACTCATCGCTGCTAG+CGG 0.629383 5:+1661146 MsG0580024181.01.T01:CDS
ATGGTGCAAGAGAGTATGCA+TGG 0.636054 5:+1664495 MsG0580024181.01.T01:CDS
AGAGAGTGAAACCTGTCACC+GGG 0.641820 5:-1661173 None:intergenic
ATTGAATACCTTGCGAAGAG+CGG 0.643228 5:-1661381 None:intergenic
GCTGCTGCTAATTTCACTCG+TGG 0.647526 5:+1661347 MsG0580024181.01.T01:CDS
CGATGAGTTTGTGTGAATCG+AGG 0.648197 5:-1661136 None:intergenic
ACGATGTATTTATGCTTGTG+CGG 0.660542 5:+1664841 MsG0580024181.01.T01:three_prime_UTR
GTGAACGAGGATGCTGTGAA+CGG 0.695181 5:-1661087 None:intergenic
TAATGGCTTCGCGATCGTGG+TGG 0.707184 5:+1661052 MsG0580024181.01.T01:CDS
ATGGACAGACACTTCCATAG+CGG 0.741310 5:+1664365 MsG0580024181.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGAAAAAAAATAAAAAG+GGG - Chr5:1662403-1662422 None:intergenic 10.0%
!!! AATCTTTTTATTGATAATAA+AGG - Chr5:1663945-1663964 None:intergenic 10.0%
!!! ATTAAAATAGTCATAATTTT+GGG - Chr5:1663184-1663203 None:intergenic 10.0%
!!! TTATCAATAAAAAGATTTTT+TGG + Chr5:1663948-1663967 MsG0580024181.01.T01:intron 10.0%
!! AAATTAGGTAAACAAAAAAA+AGG - Chr5:1661419-1661438 None:intergenic 15.0%
!! AATGTAAAGTGATTTATATT+TGG - Chr5:1663031-1663050 None:intergenic 15.0%
!! AATTAGGTAAACAAAAAAAA+GGG - Chr5:1661418-1661437 None:intergenic 15.0%
!!! AATAAAAAGATTTTTTGGAA+AGG + Chr5:1663953-1663972 MsG0580024181.01.T01:intron 15.0%
!!! ACAATTTTTATAACAAAGTT+TGG - Chr5:1664467-1664486 None:intergenic 15.0%
!!! ATTAGGGTTTTAAAAAATTA+AGG - Chr5:1662441-1662460 None:intergenic 15.0%
!!! CAAAATTATGACTATTTTAA+TGG + Chr5:1663183-1663202 MsG0580024181.01.T01:intron 15.0%
!!! CATTAAAATAGTCATAATTT+TGG - Chr5:1663185-1663204 None:intergenic 15.0%
!!! TATGTTATTTATCAATAAGT+AGG + Chr5:1663801-1663820 MsG0580024181.01.T01:intron 15.0%
!!! TGTATTTTTTATTTCACTTT+TGG + Chr5:1661549-1661568 MsG0580024181.01.T01:intron 15.0%
!!! TTACTAGTACTATATAATTT+TGG + Chr5:1663250-1663269 MsG0580024181.01.T01:intron 15.0%
!! AAGGTAATTTCAAAAATTGA+AGG + Chr5:1664774-1664793 MsG0580024181.01.T01:three_prime_UTR 20.0%
!! AATATACACAACCAATAATA+CGG - Chr5:1662813-1662832 None:intergenic 20.0%
!! AGGTAATTTCAAAAATTGAA+GGG + Chr5:1664775-1664794 MsG0580024181.01.T01:three_prime_UTR 20.0%
!! ATTAGGTAAACAAAAAAAAG+GGG - Chr5:1661417-1661436 None:intergenic 20.0%
!! CTATTCAACACTAAATATTA+GGG - Chr5:1661449-1661468 None:intergenic 20.0%
!! CTCATTTGTCTTAATTTATA+TGG + Chr5:1663860-1663879 MsG0580024181.01.T01:intron 20.0%
!! TAGATAATGATAGAACATTA+TGG - Chr5:1662562-1662581 None:intergenic 20.0%
!! TATTAGGGTTTGAAAAAATT+AGG - Chr5:1661434-1661453 None:intergenic 20.0%
!! TCTATTCAACACTAAATATT+AGG - Chr5:1661450-1661469 None:intergenic 20.0%
!! TGTTATAAAAATTGTTGACA+AGG + Chr5:1664472-1664491 MsG0580024181.01.T01:CDS 20.0%
!!! AACACTAATCATCTTTTTAT+AGG + Chr5:1663884-1663903 MsG0580024181.01.T01:intron 20.0%
!!! AGAAGTTTTTGATAAAGATA+AGG - Chr5:1664569-1664588 None:intergenic 20.0%
!!! AGTAATTTTCCTTTGTTTTT+TGG - Chr5:1663609-1663628 None:intergenic 20.0%
!!! ATAATTGATTTTGACATGTT+TGG + Chr5:1662875-1662894 MsG0580024181.01.T01:intron 20.0%
!!! ATATTTGGTTTTACTTTTAG+AGG + Chr5:1662828-1662847 MsG0580024181.01.T01:intron 20.0%
!!! ATTTTTATAACAAAGTTTGG+AGG - Chr5:1664464-1664483 None:intergenic 20.0%
!!! ATTTTTTATTTCACTTTTGG+AGG + Chr5:1661552-1661571 MsG0580024181.01.T01:intron 20.0%
!!! TATTATTGGTTGTGTATATT+TGG + Chr5:1662813-1662832 MsG0580024181.01.T01:intron 20.0%
! AAATACTGGATATATATGCT+TGG - Chr5:1663371-1663390 None:intergenic 25.0%
! AAATAGGGTTTGAATGAAAT+GGG + Chr5:1663763-1663782 MsG0580024181.01.T01:intron 25.0%
! AAATATTACTAGTACTACCT+TGG + Chr5:1663210-1663229 MsG0580024181.01.T01:intron 25.0%
! AAATTGAATCGAAAAAACCA+TGG + Chr5:1661007-1661026 MsG0580024181.01.T01:exon 25.0%
! AATACTGGATATATATGCTT+GGG - Chr5:1663370-1663389 None:intergenic 25.0%
! ACATACATAAACAATAATGC+AGG - Chr5:1661784-1661803 None:intergenic 25.0%
! AGTCAATGTAATATAGTGAT+GGG - Chr5:1664277-1664296 None:intergenic 25.0%
! AGTTTATTTACACGTGAATT+TGG + Chr5:1664191-1664210 MsG0580024181.01.T01:intron 25.0%
! ATAAAAATTGTTGACAAGGA+TGG + Chr5:1664476-1664495 MsG0580024181.01.T01:CDS 25.0%
! ATAAATTAAGACAAATGAGC+AGG - Chr5:1663859-1663878 None:intergenic 25.0%
! ATATACACAACCAATAATAC+GGG - Chr5:1662812-1662831 None:intergenic 25.0%
! ATGTTGAAATATTAAAGCTC+AGG - Chr5:1664601-1664620 None:intergenic 25.0%
! CTAATTATGTTGTAATCTTG+CGG + Chr5:1664870-1664889 MsG0580024181.01.T01:three_prime_UTR 25.0%
! GAAACAACAAAAAAAAACCA+AGG - Chr5:1663230-1663249 None:intergenic 25.0%
! GGTAATTTCAAAAATTGAAG+GGG + Chr5:1664776-1664795 MsG0580024181.01.T01:three_prime_UTR 25.0%
! GTATAGATATATGTATGATG+AGG - Chr5:1662480-1662499 None:intergenic 25.0%
! TAGTCAATGTAATATAGTGA+TGG - Chr5:1664278-1664297 None:intergenic 25.0%
! TATTAAATACAATAAGTCGC+TGG - Chr5:1662008-1662027 None:intergenic 25.0%
! TCTATAATAACAATGCAACA+TGG - Chr5:1664166-1664185 None:intergenic 25.0%
! TTACATGAGAGATTTATGTT+TGG - Chr5:1661737-1661756 None:intergenic 25.0%
! TTAGGTAAACAAAAAAAAGG+GGG - Chr5:1661416-1661435 None:intergenic 25.0%
! TTTGAATGTATTCAGTAAAC+TGG - Chr5:1661277-1661296 None:intergenic 25.0%
!! AATTCTTTTCGACAATTCTA+CGG - Chr5:1664078-1664097 None:intergenic 25.0%
!! AATTGATTCTAGCTAAAAGT+AGG - Chr5:1663057-1663076 None:intergenic 25.0%
!! AGCATATATATCCAGTATTT+TGG + Chr5:1663371-1663390 MsG0580024181.01.T01:intron 25.0%
!! GGATTTAGTTTATGTCAATT+GGG + Chr5:1662647-1662666 MsG0580024181.01.T01:intron 25.0%
!! TGATATTTTGTAGCTCAATA+AGG + Chr5:1664755-1664774 MsG0580024181.01.T01:three_prime_UTR 25.0%
!! TGGATTTAGTTTATGTCAAT+TGG + Chr5:1662646-1662665 MsG0580024181.01.T01:intron 25.0%
!! TTTGAGATTTGACAAGTATT+GGG + Chr5:1662136-1662155 MsG0580024181.01.T01:intron 25.0%
!! TTTTGATAAAGATAAGGAAC+AGG - Chr5:1664563-1664582 None:intergenic 25.0%
!!! AAGGATAGTACTGATTATTA+GGG - Chr5:1662457-1662476 None:intergenic 25.0%
!!! AGATTTCAAGTAGTAGTTTT+CGG - Chr5:1662291-1662310 None:intergenic 25.0%
!!! ATAATTGATTTTGACGTGTT+TGG + Chr5:1661599-1661618 MsG0580024181.01.T01:intron 25.0%
!!! CATCATTGATAGTGAAAAAT+AGG - Chr5:1664348-1664367 None:intergenic 25.0%
!!! CATTAGATTTTTTACACTAC+AGG + Chr5:1663458-1663477 MsG0580024181.01.T01:intron 25.0%
!!! CTATTTTTCACTATCAATGA+TGG + Chr5:1664346-1664365 MsG0580024181.01.T01:CDS 25.0%
!!! GATTTTAGCCTTGAATTTAA+TGG + Chr5:1662980-1662999 MsG0580024181.01.T01:intron 25.0%
!!! GTACTATATAATTTTGGTCT+AGG + Chr5:1663256-1663275 MsG0580024181.01.T01:intron 25.0%
!!! TAATTGATTTTGACGTGTTT+GGG + Chr5:1661600-1661619 MsG0580024181.01.T01:intron 25.0%
!!! TTTTGAGATTTGACAAGTAT+TGG + Chr5:1662135-1662154 MsG0580024181.01.T01:intron 25.0%
AAAAAGGGGTAATTTGAACT+TGG - Chr5:1662389-1662408 None:intergenic 30.0%
AAACACATGACAAGTTTAGA+TGG + Chr5:1662719-1662738 MsG0580024181.01.T01:intron 30.0%
AACACATGACAAGTTTAGAT+GGG + Chr5:1662720-1662739 MsG0580024181.01.T01:intron 30.0%
AACCTATGTCAAAAATCTCT+TGG + Chr5:1662083-1662102 MsG0580024181.01.T01:intron 30.0%
AAGGGTTAGAAAAAGTTTAC+AGG - Chr5:1662037-1662056 None:intergenic 30.0%
AAGTTGAATCTCTTTCTTTC+TGG + Chr5:1663131-1663150 MsG0580024181.01.T01:intron 30.0%
AATCTAAGCATATCAATAGC+TGG - Chr5:1664117-1664136 None:intergenic 30.0%
AATTTCAAAAATTGAAGGGG+TGG + Chr5:1664779-1664798 MsG0580024181.01.T01:three_prime_UTR 30.0%
ACTTGCAAAAATGGTTGAAT+GGG - Chr5:1663664-1663683 None:intergenic 30.0%
AGATATATGTATGATGAGGA+AGG - Chr5:1662476-1662495 None:intergenic 30.0%
ATATATGCTTGGGTTCATAT+GGG - Chr5:1663360-1663379 None:intergenic 30.0%
ATCATCACTCCAAAAAACAA+AGG + Chr5:1663597-1663616 MsG0580024181.01.T01:intron 30.0%
CAGTTGAAGATGAAATAAAG+AGG + Chr5:1662200-1662219 MsG0580024181.01.T01:intron 30.0%
CTTTGTATCAATTCCGTAAT+GGG + Chr5:1661306-1661325 MsG0580024181.01.T01:CDS 30.0%
GAAATAGGGTTTGAATGAAA+TGG + Chr5:1663762-1663781 MsG0580024181.01.T01:intron 30.0%
GGATACATTTGTGTAAAAGT+GGG - Chr5:1663010-1663029 None:intergenic 30.0%
TATATATGCTTGGGTTCATA+TGG - Chr5:1663361-1663380 None:intergenic 30.0%
TATTTCTCTCAAAATTTGGG+GGG - Chr5:1663748-1663767 None:intergenic 30.0%
TGGATACATTTGTGTAAAAG+TGG - Chr5:1663011-1663030 None:intergenic 30.0%
! ACCCTATTTCTCTCAAAATT+TGG - Chr5:1663752-1663771 None:intergenic 30.0%
! CCCTATTTCTCTCAAAATTT+GGG - Chr5:1663751-1663770 None:intergenic 30.0%
! CCTATTTCTCTCAAAATTTG+GGG - Chr5:1663750-1663769 None:intergenic 30.0%
! CTATTTCTCTCAAAATTTGG+GGG - Chr5:1663749-1663768 None:intergenic 30.0%
! CTCTTTATTTCATCTTCAAC+TGG - Chr5:1662202-1662221 None:intergenic 30.0%
! TACCAAGAGATTTTTGACAT+AGG - Chr5:1662088-1662107 None:intergenic 30.0%
! TTGAGATTTGACAAGTATTG+GGG + Chr5:1662137-1662156 MsG0580024181.01.T01:intron 30.0%
! TTGTTTTCACTTTATCGTAG+TGG + Chr5:1664622-1664641 MsG0580024181.01.T01:three_prime_UTR 30.0%
!! ATTGATTTTAAACCGAAGCT+AGG + Chr5:1661637-1661656 MsG0580024181.01.T01:intron 30.0%
!! CATTCGTTTTTCACTTTTCA+GGG + Chr5:1661250-1661269 MsG0580024181.01.T01:intron 30.0%
!! CCCAAATTTTGAGAGAAATA+GGG + Chr5:1663748-1663767 MsG0580024181.01.T01:intron 30.0%
!! GAAGGATAGTACTGATTATT+AGG - Chr5:1662458-1662477 None:intergenic 30.0%
!! TACACCTCTAGAATCAAATT+TGG - Chr5:1662856-1662875 None:intergenic 30.0%
!! TCATTCGTTTTTCACTTTTC+AGG + Chr5:1661249-1661268 MsG0580024181.01.T01:intron 30.0%
!! TCTTGTTGATAAGTGCATTT+TGG + Chr5:1664412-1664431 MsG0580024181.01.T01:CDS 30.0%
!! TGTTTCTATCTCCTTTTGAA+CGG - Chr5:1662704-1662723 None:intergenic 30.0%
!!! ATATCCAGTATTTTGGTTGA+GGG + Chr5:1663378-1663397 MsG0580024181.01.T01:intron 30.0%
!!! GTTTTTTGGAGTGATGATTA+TGG - Chr5:1663595-1663614 None:intergenic 30.0%
!!! TATATCCAGTATTTTGGTTG+AGG + Chr5:1663377-1663396 MsG0580024181.01.T01:intron 30.0%
AAAGCTACAAATCCTAGCTT+CGG - Chr5:1661652-1661671 None:intergenic 35.0%
AAATAGGTTGTGGCTAATGA+AGG - Chr5:1663569-1663588 None:intergenic 35.0%
AAGGGTTATCTCTCATACAA+TGG - Chr5:1662240-1662259 None:intergenic 35.0%
AATAGGTTGTGGCTAATGAA+GGG - Chr5:1663568-1663587 None:intergenic 35.0%
AATCTTGAAGCTTAGTGACA+GGG + Chr5:1662107-1662126 MsG0580024181.01.T01:intron 35.0%
ACAAATGAGCAGGAACTATT+AGG - Chr5:1663849-1663868 None:intergenic 35.0%
ACACTTATGACTTACCTATC+CGG + Chr5:1664044-1664063 MsG0580024181.01.T01:intron 35.0%
ACACTTCACAAGCTTGAAAA+GGG + Chr5:1664305-1664324 MsG0580024181.01.T01:CDS 35.0%
ACGATGTATTTATGCTTGTG+CGG + Chr5:1664841-1664860 MsG0580024181.01.T01:three_prime_UTR 35.0%
AGAGATTCACAAACTGAAGT+AGG - Chr5:1662175-1662194 None:intergenic 35.0%
AGGAATCTTAAAGGACTTTC+AGG + Chr5:1663425-1663444 MsG0580024181.01.T01:intron 35.0%
AGTCTGAGCCAAACTTAATA+AGG + Chr5:1663681-1663700 MsG0580024181.01.T01:intron 35.0%
ATGAGTTCGTTTGATCCATA+TGG + Chr5:1661493-1661512 MsG0580024181.01.T01:intron 35.0%
ATGTATCTGAAAACCTTGCA+TGG + Chr5:1664652-1664671 MsG0580024181.01.T01:three_prime_UTR 35.0%
CAATTCTACGGATCAATATC+CGG - Chr5:1664066-1664085 None:intergenic 35.0%
GACTTGCAAAAATGGTTGAA+TGG - Chr5:1663665-1663684 None:intergenic 35.0%
GCTTTGTATCAATTCCGTAA+TGG + Chr5:1661305-1661324 MsG0580024181.01.T01:CDS 35.0%
GTAGATCAACAAGTGAAGAA+AGG + Chr5:1661808-1661827 MsG0580024181.01.T01:intron 35.0%
GTTCTGTTCAGGAATCTTAA+AGG + Chr5:1663416-1663435 MsG0580024181.01.T01:intron 35.0%
TAATCTTGAAGCTTAGTGAC+AGG + Chr5:1662106-1662125 MsG0580024181.01.T01:intron 35.0%
TCCCAAAACACATAAGAACA+CGG + Chr5:1663511-1663530 MsG0580024181.01.T01:intron 35.0%
TTAAGATTCCTGAACAGAAC+AGG - Chr5:1663416-1663435 None:intergenic 35.0%
TTGTTTGATATGAAACCTGC+TGG - Chr5:1661884-1661903 None:intergenic 35.0%
TTTGGAAATCAGATCAAGGT+TGG + Chr5:1664430-1664449 MsG0580024181.01.T01:CDS 35.0%
TTTGGCATGTCATTCTACAA+GGG - Chr5:1662258-1662277 None:intergenic 35.0%
! AAAACCATGGAATGCGTTTT+CGG + Chr5:1661020-1661039 MsG0580024181.01.T01:exon 35.0%
! ACATAGGTTTTCAGTTTCCA+GGG - Chr5:1662072-1662091 None:intergenic 35.0%
! ACCGTGTTCTTATGTGTTTT+GGG - Chr5:1663515-1663534 None:intergenic 35.0%
! ATTATGGTGGAAATAGGTTG+TGG - Chr5:1663579-1663598 None:intergenic 35.0%
! GTGATGATTATGGTGGAAAT+AGG - Chr5:1663585-1663604 None:intergenic 35.0%
! TCTTTTTCTGTGTATGTGAC+TGG - Chr5:1663996-1664015 None:intergenic 35.0%
! TGGGTTGACCATTAAATTCA+AGG - Chr5:1662991-1663010 None:intergenic 35.0%
!! AATAGTGCTTTAGGGCTTAT+TGG + Chr5:1664802-1664821 MsG0580024181.01.T01:three_prime_UTR 35.0%
!! CCCCAAATTTTGAGAGAAAT+AGG + Chr5:1663747-1663766 MsG0580024181.01.T01:intron 35.0%
!! CTAAGTTAAGTTGATGATGG+AGG - Chr5:1663544-1663563 None:intergenic 35.0%
!! GCATTTTGGAAATCAGATCA+AGG + Chr5:1664426-1664445 MsG0580024181.01.T01:CDS 35.0%
!! GGACTAAGTTAAGTTGATGA+TGG - Chr5:1663547-1663566 None:intergenic 35.0%
!!! GCATTTTTTTTTCCCCATAC+TGG + Chr5:1662587-1662606 MsG0580024181.01.T01:intron 35.0%
!!! GTTTTCGGATTAGTTGGTAA+TGG + Chr5:1661035-1661054 MsG0580024181.01.T01:CDS 35.0%
!!! TATTTTGGTTGAGGGAAGTT+TGG + Chr5:1663386-1663405 MsG0580024181.01.T01:intron 35.0%
!!! TTTTGGAGTGATGATTATGG+TGG - Chr5:1663592-1663611 None:intergenic 35.0%
AAACCTTGCATGGTCTCTAA+TGG + Chr5:1664662-1664681 MsG0580024181.01.T01:three_prime_UTR 40.0%
AAACTTAATAAGGCTGCAGG+TGG + Chr5:1663691-1663710 MsG0580024181.01.T01:intron 40.0%
AAACTTTGGATGGGAAGAGA+TGG + Chr5:1662339-1662358 MsG0580024181.01.T01:intron 40.0%
AACTTAATAAGGCTGCAGGT+GGG + Chr5:1663692-1663711 MsG0580024181.01.T01:intron 40.0%
AACTTTGGATGGGAAGAGAT+GGG + Chr5:1662340-1662359 MsG0580024181.01.T01:intron 40.0%
AAGAAGGATGTTCACAGAGT+AGG - Chr5:1664231-1664250 None:intergenic 40.0%
AAGTCCTGTTTCAGTAAGTG+GGG - Chr5:1661523-1661542 None:intergenic 40.0%
AATGAACAGATCACAGATGG+TGG - Chr5:1663635-1663654 None:intergenic 40.0%
ACACAACCAATAATACGGGA+AGG - Chr5:1662808-1662827 None:intergenic 40.0%
ACTTCCCTCAACCAAAATAC+TGG - Chr5:1663385-1663404 None:intergenic 40.0%
ACTTTCATGTTAGTTCCAGC+AGG + Chr5:1661866-1661885 MsG0580024181.01.T01:intron 40.0%
AGAAGGATGTTCACAGAGTA+GGG - Chr5:1664230-1664249 None:intergenic 40.0%
AGAATCCCTTCCCGTATTAT+TGG + Chr5:1662799-1662818 MsG0580024181.01.T01:intron 40.0%
ATCAAACAAACTCTGCCAGT+TGG + Chr5:1661895-1661914 MsG0580024181.01.T01:intron 40.0%
ATCAACCCATGAAACCAGTA+TGG - Chr5:1662604-1662623 None:intergenic 40.0%
ATCTCTTCCCATCCAAAGTT+TGG - Chr5:1662340-1662359 None:intergenic 40.0%
ATTGAATACCTTGCGAAGAG+CGG - Chr5:1661384-1661403 None:intergenic 40.0%
CAAGTCCTGTTTCAGTAAGT+GGG - Chr5:1661524-1661543 None:intergenic 40.0%
CACAACCAATAATACGGGAA+GGG - Chr5:1662807-1662826 None:intergenic 40.0%
CAGCTAACCAAACTTTGGAT+GGG + Chr5:1662330-1662349 MsG0580024181.01.T01:intron 40.0%
CTTTGGCATGTCATTCTACA+AGG - Chr5:1662259-1662278 None:intergenic 40.0%
CTTTGTCATAACCAGCAAGA+AGG - Chr5:1664247-1664266 None:intergenic 40.0%
GAACATGATCTTGTCTTCGT+TGG - Chr5:1661117-1661136 None:intergenic 40.0%
GACACTTCACAAGCTTGAAA+AGG + Chr5:1664304-1664323 MsG0580024181.01.T01:CDS 40.0%
GGAGCCAAATTTGATTCTAG+AGG + Chr5:1662849-1662868 MsG0580024181.01.T01:intron 40.0%
GGATTAAATCAAGCTGAAGC+AGG - Chr5:1664542-1664561 None:intergenic 40.0%
GTCAATGAACAGATCACAGA+TGG - Chr5:1663638-1663657 None:intergenic 40.0%
TAACCCAGCTAACCAAACTT+TGG + Chr5:1662325-1662344 MsG0580024181.01.T01:intron 40.0%
TAATCCGAAAACGCATTCCA+TGG - Chr5:1661027-1661046 None:intergenic 40.0%
TAGTTGACATGCGAATGGTT+TGG + Chr5:1661918-1661937 MsG0580024181.01.T01:intron 40.0%
TCAACCCATGAAACCAGTAT+GGG - Chr5:1662603-1662622 None:intergenic 40.0%
TTCAGTTTCCAGGGTTATTG+AGG - Chr5:1662063-1662082 None:intergenic 40.0%
TTGGCTCAGACTTGCAAAAA+TGG - Chr5:1663673-1663692 None:intergenic 40.0%
! AAGCTTGAAAAGGGAGCTTT+TGG + Chr5:1664314-1664333 MsG0580024181.01.T01:CDS 40.0%
! AATCAGATCAAGGTTGGTTG+TGG + Chr5:1664436-1664455 MsG0580024181.01.T01:CDS 40.0%
! CACCGTGTTCTTATGTGTTT+TGG - Chr5:1663516-1663535 None:intergenic 40.0%
! CATCCAAAGTTTGGTTAGCT+GGG - Chr5:1662331-1662350 None:intergenic 40.0%
! CTTTCTTTTACTGTCAGTGC+TGG + Chr5:1663917-1663936 MsG0580024181.01.T01:intron 40.0%
! GACATAGGTTTTCAGTTTCC+AGG - Chr5:1662073-1662092 None:intergenic 40.0%
! GGATACTTTTGTGCTAGAGT+GGG - Chr5:1661716-1661735 None:intergenic 40.0%
! TGAATCTCTTTCTTTCTGGC+TGG + Chr5:1663135-1663154 MsG0580024181.01.T01:intron 40.0%
! TGGATACTTTTGTGCTAGAG+TGG - Chr5:1661717-1661736 None:intergenic 40.0%
!! ACCTGCAGCCTTATTAAGTT+TGG - Chr5:1663692-1663711 None:intergenic 40.0%
!! GAAAAGGGAGCTTTTGGTTA+TGG + Chr5:1664320-1664339 MsG0580024181.01.T01:CDS 40.0%
!! TTGACAAGTATTGGGGTTAG+TGG + Chr5:1662144-1662163 MsG0580024181.01.T01:intron 40.0%
!! TTGGTTTTCCTGTTCTGTTC+AGG + Chr5:1663405-1663424 MsG0580024181.01.T01:intron 40.0%
!! TTTCCCCATACTGGTTTCAT+GGG + Chr5:1662596-1662615 MsG0580024181.01.T01:intron 40.0%
!!! GAATGCGTTTTCGGATTAGT+TGG + Chr5:1661029-1661048 MsG0580024181.01.T01:CDS 40.0%
!!! TTTTCCCCATACTGGTTTCA+TGG + Chr5:1662595-1662614 MsG0580024181.01.T01:intron 40.0%
AAGAGAGTGAAACCTGTCAC+CGG - Chr5:1661177-1661196 None:intergenic 45.0%
AAGCCTTAACCCACTAAGTG+GGG + Chr5:1662513-1662532 MsG0580024181.01.T01:intron 45.0%
AAGGGGTGGAATAGTGCTTT+AGG + Chr5:1664793-1664812 MsG0580024181.01.T01:three_prime_UTR 45.0%
ACTTAATAAGGCTGCAGGTG+GGG + Chr5:1663693-1663712 MsG0580024181.01.T01:intron 45.0%
AGGGGTGGAATAGTGCTTTA+GGG + Chr5:1664794-1664813 MsG0580024181.01.T01:three_prime_UTR 45.0%
ATCCCTGTTATAGCCATCGT+AGG + Chr5:1661832-1661851 MsG0580024181.01.T01:intron 45.0%
ATGGACAGACACTTCCATAG+CGG + Chr5:1664365-1664384 MsG0580024181.01.T01:CDS 45.0%
CAACCCATGAAACCAGTATG+GGG - Chr5:1662602-1662621 None:intergenic 45.0%
CAAGCCTTAACCCACTAAGT+GGG + Chr5:1662512-1662531 MsG0580024181.01.T01:intron 45.0%
CACCTACGATGGCTATAACA+GGG - Chr5:1661837-1661856 None:intergenic 45.0%
CCAAGTCCTGTTTCAGTAAG+TGG - Chr5:1661525-1661544 None:intergenic 45.0%
CCACTTACTGAAACAGGACT+TGG + Chr5:1661522-1661541 MsG0580024181.01.T01:intron 45.0%
CCAGCTAACCAAACTTTGGA+TGG + Chr5:1662329-1662348 MsG0580024181.01.T01:intron 45.0%
CCAGGGTTATTGAGGTTCAA+GGG - Chr5:1662055-1662074 None:intergenic 45.0%
CCATCCAAAGTTTGGTTAGC+TGG - Chr5:1662332-1662351 None:intergenic 45.0%
CGATGAGTTTGTGTGAATCG+AGG - Chr5:1661139-1661158 None:intergenic 45.0%
CTACGGATCAATATCCGGAT+AGG - Chr5:1664061-1664080 None:intergenic 45.0%
CTTCGTTGGATTTGTGAACG+AGG - Chr5:1661103-1661122 None:intergenic 45.0%
GAATAGTCTAAGCGTGCGTT+TGG - Chr5:1663109-1663128 None:intergenic 45.0%
GCCAAACTTAATAAGGCTGC+AGG + Chr5:1663688-1663707 MsG0580024181.01.T01:intron 45.0%
GCTGGTTATGACAAAGAGAC+AGG + Chr5:1664251-1664270 MsG0580024181.01.T01:CDS 45.0%
GTCATAAGTGTGCTGTGTCA+TGG - Chr5:1664035-1664054 None:intergenic 45.0%
GTGATGTCTCACCGTTCAAA+AGG + Chr5:1662690-1662709 MsG0580024181.01.T01:intron 45.0%
TCAAGCCTTAACCCACTAAG+TGG + Chr5:1662511-1662530 MsG0580024181.01.T01:intron 45.0%
TCACCTACGATGGCTATAAC+AGG - Chr5:1661838-1661857 None:intergenic 45.0%
TCCAGGGTTATTGAGGTTCA+AGG - Chr5:1662056-1662075 None:intergenic 45.0%
TCTGTGAACATCCTTCTTGC+TGG + Chr5:1664233-1664252 MsG0580024181.01.T01:CDS 45.0%
TGGTGAGAGGAATCCCATTA+CGG - Chr5:1661322-1661341 None:intergenic 45.0%
TTCGCATGTCAACTACCAAC+TGG - Chr5:1661913-1661932 None:intergenic 45.0%
! ATGGTGCAAGAGAGTATGCA+TGG + Chr5:1664495-1664514 MsG0580024181.01.T01:CDS 45.0%
! GGTGTCGAGATTGATTCTAG+AGG + Chr5:1661573-1661592 MsG0580024181.01.T01:intron 45.0%
! GTTTTCGGCTCTCTCAACTT+TGG - Chr5:1662276-1662295 None:intergenic 45.0%
!! ATCTCTTTCTTTCTGGCTGG+AGG + Chr5:1663138-1663157 MsG0580024181.01.T01:intron 45.0%
!! GTTGGTAGTTGACATGCGAA+TGG + Chr5:1661913-1661932 MsG0580024181.01.T01:intron 45.0%
!! AAATTAGAAAAAAAATAAAA+AGG - Chr5:1662405-1662424 None:intergenic 5.0%
!! AATGTTAAAAATTTAATATT+TGG + Chr5:1662626-1662645 MsG0580024181.01.T01:intron 5.0%
!! AATTAGAAAAAAAATAAAAA+GGG - Chr5:1662404-1662423 None:intergenic 5.0%
AGAGAGTGAAACCTGTCACC+GGG - Chr5:1661176-1661195 None:intergenic 50.0%
AGTGTGCTGTGTCATGGTGA+TGG - Chr5:1664029-1664048 None:intergenic 50.0%
ATGTATCCGACCCCACTTAG+TGG - Chr5:1662526-1662545 None:intergenic 50.0%
CACAAACTCATCGCTGCTAG+CGG + Chr5:1661146-1661165 MsG0580024181.01.T01:CDS 50.0%
CCCTTGAACCTCAATAACCC+TGG + Chr5:1662052-1662071 MsG0580024181.01.T01:intron 50.0%
CCTGTTTCAGTAAGTGGGGT+CGG - Chr5:1661519-1661538 None:intergenic 50.0%
CGACCATTAGAGACCATGCA+AGG - Chr5:1664668-1664687 None:intergenic 50.0%
CTTAACCCACTAAGTGGGGT+CGG + Chr5:1662517-1662536 MsG0580024181.01.T01:intron 50.0%
CTTCCACGTTCATTCCGCTA+TGG - Chr5:1664382-1664401 None:intergenic 50.0%
GAAATTAGCAGCAGCAGCTG+TGG - Chr5:1661342-1661361 None:intergenic 50.0%
GCTGCTGCTAATTTCACTCG+TGG + Chr5:1661347-1661366 MsG0580024181.01.T01:CDS 50.0%
GGATACCATCATCTCTCGCT+CGG - Chr5:1662368-1662387 None:intergenic 50.0%
GTGAACGAGGATGCTGTGAA+CGG - Chr5:1661090-1661109 None:intergenic 50.0%
GTGTGCTGTGTCATGGTGAT+GGG - Chr5:1664028-1664047 None:intergenic 50.0%
TGTATCCGACCCCACTTAGT+GGG - Chr5:1662525-1662544 None:intergenic 50.0%
! CTTCCATAGCGGAATGAACG+TGG + Chr5:1664376-1664395 MsG0580024181.01.T01:CDS 50.0%
! TGTGCTGTGTCATGGTGATG+GGG - Chr5:1664027-1664046 None:intergenic 50.0%
AGTAAGTGGGGTCGGCCATA+TGG - Chr5:1661511-1661530 None:intergenic 55.0%
CCGACCCCACTTACTGAAAC+AGG + Chr5:1661516-1661535 MsG0580024181.01.T01:intron 55.0%
GAATACCTTGCGAAGAGCGG+TGG - Chr5:1661381-1661400 None:intergenic 55.0%
GTGACTGCTCTCACCTACGA+TGG - Chr5:1661848-1661867 None:intergenic 55.0%
TAATGGCTTCGCGATCGTGG+TGG + Chr5:1661052-1661071 MsG0580024181.01.T01:CDS 55.0%
TGCATGGCGCGAATCAGTCA+AGG + Chr5:1664511-1664530 MsG0580024181.01.T01:CDS 55.0%
!! CGACCCCACTTAGTGGGTTA+AGG - Chr5:1662519-1662538 None:intergenic 55.0%
!! GTGCTGTGTCATGGTGATGG+GGG - Chr5:1664026-1664045 None:intergenic 55.0%
!! TGGTAATGGCTTCGCGATCG+TGG + Chr5:1661049-1661068 MsG0580024181.01.T01:CDS 55.0%
ATCGCTGCTAGCGGTGAACC+CGG + Chr5:1661155-1661174 MsG0580024181.01.T01:CDS 60.0%
GGGAACCGAGCGAGAGATGA+TGG + Chr5:1662360-1662379 MsG0580024181.01.T01:intron 60.0%
TAAGGCTGCAGGTGGGGTAC+TGG + Chr5:1663699-1663718 MsG0580024181.01.T01:intron 60.0%
!! GTGGGGTACTGGTGGTGGAA+GGG + Chr5:1663710-1663729 MsG0580024181.01.T01:intron 60.0%
CTAGCGGTGAACCCGGTGAC+AGG + Chr5:1661162-1661181 MsG0580024181.01.T01:CDS 65.0%
GCAGCAGCAGCTGTGGTGAG+AGG - Chr5:1661335-1661354 None:intergenic 65.0%
GCTTGCCACCGCTCTTCGCA+AGG + Chr5:1661373-1661392 MsG0580024181.01.T01:CDS 65.0%
!! GGTGGGGTACTGGTGGTGGA+AGG + Chr5:1663709-1663728 MsG0580024181.01.T01:intron 65.0%
!! TGCAGGTGGGGTACTGGTGG+TGG + Chr5:1663705-1663724 MsG0580024181.01.T01:intron 65.0%
!! GGCTGCAGGTGGGGTACTGG+TGG + Chr5:1663702-1663721 MsG0580024181.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 1660929 1664908 1660929 ID=MsG0580024181.01;Name=MsG0580024181.01
Chr5 mRNA 1660929 1664908 1660929 ID=MsG0580024181.01.T01;Parent=MsG0580024181.01;Name=MsG0580024181.01.T01;_AED=0.44;_eAED=0.44;_QI=97|1|1|1|1|1|3|355|203
Chr5 exon 1660929 1661183 1660929 ID=MsG0580024181.01.T01:exon:475;Parent=MsG0580024181.01.T01
Chr5 exon 1661271 1661394 1661271 ID=MsG0580024181.01.T01:exon:476;Parent=MsG0580024181.01.T01
Chr5 exon 1664224 1664908 1664224 ID=MsG0580024181.01.T01:exon:477;Parent=MsG0580024181.01.T01
Chr5 five_prime_UTR 1660929 1661025 1660929 ID=MsG0580024181.01.T01:five_prime_utr;Parent=MsG0580024181.01.T01
Chr5 CDS 1661026 1661183 1661026 ID=MsG0580024181.01.T01:cds;Parent=MsG0580024181.01.T01
Chr5 CDS 1661271 1661394 1661271 ID=MsG0580024181.01.T01:cds;Parent=MsG0580024181.01.T01
Chr5 CDS 1664224 1664553 1664224 ID=MsG0580024181.01.T01:cds;Parent=MsG0580024181.01.T01
Chr5 three_prime_UTR 1664554 1664908 1664554 ID=MsG0580024181.01.T01:three_prime_utr;Parent=MsG0580024181.01.T01
Gene Sequence

>MsG0580024181.01.T01

ATGGAATGCGTTTTCGGATTAGTTGGTAATGGCTTCGCGATCGTGGTGGCTGATACATCTGCCGTTCACAGCATCCTCGTTCACAAATCCAACGAAGACAAGATCATGTTCCTCGATTCACACAAACTCATCGCTGCTAGCGGTGAACCCGGTGACAGGGTCCAGTTTACTGAATACATTCAAAAGAACGTTGCTTTGTATCAATTCCGTAATGGGATTCCTCTCACCACAGCTGCTGCTGCTAATTTCACTCGTGGCGAGCTTGCCACCGCTCTTCGCAAGAATCCCTACTCTGTGAACATCCTTCTTGCTGGTTATGACAAAGAGACAGGCCCATCACTATATTACATTGACTACATTGCGACACTTCACAAGCTTGAAAAGGGAGCTTTTGGTTATGGTTCCTATTTTTCACTATCAATGATGGACAGACACTTCCATAGCGGAATGAACGTGGAAGAAGCAATTGATCTTGTTGATAAGTGCATTTTGGAAATCAGATCAAGGTTGGTTGTGGCACCTCCAAACTTTGTTATAAAAATTGTTGACAAGGATGGTGCAAGAGAGTATGCATGGCGCGAATCAGTCAAGGACACTCCTGCTTCAGCTTGA
Protein sequence

>MsG0580024181.01.T01

MECVFGLVGNGFAIVVADTSAVHSILVHKSNEDKIMFLDSHKLIAASGEPGDRVQFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYIATLHKLEKGAFGYGSYFSLSMMDRHFHSGMNVEEAIDLVDKCILEIRSRLVVAPPNFVIKIVDKDGAREYAWRESVKDTPASA*