AlfalfaGEDB | MsG0680035574.01

M. sativa cultivar ZhongmuNo.1 / MsG0680035574.01

KEGG: K02736
GO: GO:0004175 GO:0004298 GO:0005839 GO:0051603
PFAM: PF00227

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035574.01.T01	XP_003620482.1	100	232	0	0	1	232	1	232	7.43E-171	479
MsG0680035574.01.T02	XP_003620482.1	100	232	0	0	1	232	1	232	7.43E-171	479

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035574.01.T01	Q7DLR9	83.7	227	37	0	6	232	20	246	4.22E-147	412
MsG0680035574.01.T02	Q7DLR9	83.7	227	37	0	6	232	20	246	4.22E-147	412

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035574.01.T01	G7KN41	100.000	232	0	0	1	232	1	232	3.55e-171	479
MsG0680035574.01.T02	G7KN41	100.000	232	0	0	1	232	1	232	3.55e-171	479

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0680035574.01	0.858486	7.970189e-63	2.037527e-59
MsG0080048058.01	MsG0680035574.01	0.811279	7.579313e-51	4.671782e-48
MsG0080048383.01	MsG0680035574.01	0.862521	4.776860e-64	1.403067e-60
MsG0080048687.01	MsG0680035574.01	0.829609	4.676872e-55	4.810704e-52
MsG0080048784.01	MsG0680035574.01	0.814857	1.244091e-51	8.442473e-49
MsG0080048855.01	MsG0680035574.01	0.808458	3.066773e-50	1.754483e-47
MsG0080048968.01	MsG0680035574.01	0.821346	4.239780e-53	3.440462e-50
MsG0180000001.01	MsG0680035574.01	0.851920	6.481990e-61	1.332123e-57
MsG0180000101.01	MsG0680035574.01	0.847975	8.229508e-60	1.487851e-56
MsG0180000135.01	MsG0680035574.01	0.818928	1.517437e-52	1.151476e-49

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PPI

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Gene1	Gene2	Type
MsG0180006029.01	MsG0680035574.01	PPI
MsG0180006029.01	MsG0680035574.01	PPI
MsG0180006029.01	MsG0680035574.01	PPI
MsG0180006029.01	MsG0680035574.01	PPI
MsG0280009575.01	MsG0680035574.01	PPI
MsG0280009575.01	MsG0680035574.01	PPI
MsG0280009575.01	MsG0680035574.01	PPI
MsG0280009575.01	MsG0680035574.01	PPI
MsG0280011059.01	MsG0680035574.01	PPI
MsG0280011059.01	MsG0680035574.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035574.01.T01	MTR_6g084450	100.000	232	0	1	232	1	232	9.00e-175	479
MsG0680035574.01.T01	MTR_4g060540	91.810	232	19	1	232	1	232	6.23e-165	454
MsG0680035574.01.T02	MTR_6g084450	100.000	232	0	1	232	1	232	9.00e-175	479
MsG0680035574.01.T02	MTR_4g060540	91.810	232	19	1	232	1	232	6.23e-165	454

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035574.01.T01	AT1G56450	83.700	227	37	0	6	232	20	246	4.30e-148	412
MsG0680035574.01.T02	AT1G56450	83.700	227	37	0	6	232	20	246	4.30e-148	412

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Find 34 sgRNAs with CRISPR-Local

Find 351 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAATAAAGATGACAA+AGG	0.641574	6:+108647443	None:intergenic
AAGATTTGAGTCATACATGC+CGG	0.543443	6:+108645495	None:intergenic
ACAACATAGCTTAAATATAT+GGG	0.392128	6:-108645150	MsG0680035574.01.T01:five_prime_UTR
ACACACACACATGATAATTA+TGG	0.204723	6:+108645829	None:intergenic
AGAATACATACAGAGTTCTC+TGG	0.424755	6:+108644839	None:intergenic
AGATTTGAGTCATACATGCC+GGG	0.548316	6:+108645496	None:intergenic
AGTGTCCATCACAACGACGA+AGG	0.686041	6:+108644873	None:intergenic
ATAGAATCCCTTGCAAATAA+AGG	0.349694	6:+108645676	None:intergenic
ATCACGAACTCTCCGATTCA+AGG	0.441934	6:-108647370	MsG0680035574.01.T02:intron
ATTGTGCTAGAACAGCCCCT+AGG	0.649598	6:-108645771	MsG0680035574.01.T01:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAGTAAAATGAAGCTGA+TGG	+	Chr6:108644938-108644957	None:intergenic	30.0%
AACTAATTCACAGGTTCATA+TGG	-	Chr6:108644686-108644705	MsG0680035574.01.T01:CDS	30.0%
AAGAAGTAAAAACTGAACAG+GGG	-	Chr6:108646308-108646327	MsG0680035574.01.T02:intron	30.0%
AAGCATTTCTTAAGACTGTT+TGG	+	Chr6:108642126-108642145	None:intergenic	30.0%
AATAAGAATGAGGGTTTATC+AGG	+	Chr6:108641651-108641670	None:intergenic	30.0%
AATTGTATATAGGGTCTGTT+TGG	-	Chr6:108642821-108642840	MsG0680035574.01.T01:intron	30.0%
ACAACAAACAAAAATCAGGA+AGG	+	Chr6:108645289-108645308	None:intergenic	30.0%
ACAACACTGAATAGTTATGT+TGG	+	Chr6:108647270-108647289	None:intergenic	30.0%
ACACACACACATGATAATTA+TGG	+	Chr6:108643079-108643098	None:intergenic	30.0%
ACAGTCTTAAGAAATGCTTA+TGG	-	Chr6:108642127-108642146	MsG0680035574.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	108641425	108647502	108641425	ID=MsG0680035574.01;Name=MsG0680035574.01
Chr6	mRNA	108641425	108646038	108641425	ID=MsG0680035574.01.T01;Parent=MsG0680035574.01;Name=MsG0680035574.01.T01;_AED=0.33;_eAED=0.33;_QI=1137\|1\|1\|1\|0.83\|0.71\|7\|279\|232
Chr6	exon	108644850	108644962	108644850	ID=MsG0680035574.01.T01:exon:14479;Parent=MsG0680035574.01.T01
Chr6	exon	108644983	108646038	108644983	ID=MsG0680035574.01.T01:exon:14478;Parent=MsG0680035574.01.T01
Chr6	five_prime_UTR	108644983	108646038	108644983	ID=MsG0680035574.01.T01:five_prime_utr;Parent=MsG0680035574.01.T01
Chr6	five_prime_UTR	108644882	108644962	108644882	ID=MsG0680035574.01.T01:five_prime_utr;Parent=MsG0680035574.01.T01
Chr6	CDS	108644850	108644881	108644850	ID=MsG0680035574.01.T01:cds;Parent=MsG0680035574.01.T01
Chr6	CDS	108644647	108644723	108644647	ID=MsG0680035574.01.T01:cds;Parent=MsG0680035574.01.T01
Chr6	CDS	108644099	108644228	108644099	ID=MsG0680035574.01.T01:cds;Parent=MsG0680035574.01.T01
Chr6	CDS	108643686	108643845	108643686	ID=MsG0680035574.01.T01:cds;Parent=MsG0680035574.01.T01
Chr6	CDS	108643414	108643599	108643414	ID=MsG0680035574.01.T01:cds;Parent=MsG0680035574.01.T01
Chr6	CDS	108641704	108641817	108641704	ID=MsG0680035574.01.T01:cds;Parent=MsG0680035574.01.T01
Chr6	three_prime_UTR	108641425	108641703	108641425	ID=MsG0680035574.01.T01:three_prime_utr;Parent=MsG0680035574.01.T01
Chr6	mRNA	108641425	108647502	108641425	ID=MsG0680035574.01.T02;Parent=MsG0680035574.01;Name=MsG0680035574.01.T02;_AED=0.41;_eAED=0.41;_QI=166\|1\|1\|1\|0.83\|0.71\|7\|279\|232
Chr6	exon	108644850	108644915	108644850	ID=MsG0680035574.01.T02:exon:14486;Parent=MsG0680035574.01.T02
Chr6	exon	108647371	108647502	108647371	ID=MsG0680035574.01.T02:exon:14485;Parent=MsG0680035574.01.T02
Chr6	five_prime_UTR	108647371	108647502	108647371	ID=MsG0680035574.01.T02:five_prime_utr;Parent=MsG0680035574.01.T02
Chr6	five_prime_UTR	108644882	108644915	108644882	ID=MsG0680035574.01.T02:five_prime_utr;Parent=MsG0680035574.01.T02
Chr6	CDS	108644850	108644881	108644850	ID=MsG0680035574.01.T02:cds;Parent=MsG0680035574.01.T02
Chr6	CDS	108644647	108644723	108644647	ID=MsG0680035574.01.T02:cds;Parent=MsG0680035574.01.T02
Chr6	CDS	108644099	108644228	108644099	ID=MsG0680035574.01.T02:cds;Parent=MsG0680035574.01.T02
Chr6	CDS	108643686	108643845	108643686	ID=MsG0680035574.01.T02:cds;Parent=MsG0680035574.01.T02
Chr6	CDS	108643414	108643599	108643414	ID=MsG0680035574.01.T02:cds;Parent=MsG0680035574.01.T02
Chr6	CDS	108641704	108641817	108641704	ID=MsG0680035574.01.T02:cds;Parent=MsG0680035574.01.T02
Chr6	three_prime_UTR	108641425	108641703	108641425	ID=MsG0680035574.01.T02:three_prime_utr;Parent=MsG0680035574.01.T02

Gene Sequence

>MsG0680035574.01.T01

ATGGACACTGTAACTGAATCCCAGAGAACTCTGTACCCATACGTAACTGGATCCTCCGTCGTTGCTATCAAATACAAAGATGGCATTCTCATGGCTGCTGATATGGGAGGTTCATATGGATCTACACTGAGGTACAAGAGTGTTGAGCGTTTGAAGCCTATTGGAAAGCACTCCCTTCTTGGTGCTAGTGGGGAAATAAGTGACTTTCAGGAGATTATGCGCTACCTTGATGAGCTCATCCTTATTGACAACATGTGGGATGATGGGAACTCTTTGGGACCTAAGGAGGTCCACAACTATTTAACTCGGGTGATGTATAATAGGCGCAACAAGTTCAATCCATTGTGGAACTCACTTGTTCTTGGTGGTGTTAAAAAGGGGCAGAAGTACCTTGGCACGGTTAGCATGATTGGCGTTAATTACGAAGACAACCATGTGGCAACTGGGCTTGGAAATCATCTTGCTAGGCCAATTCTCCGCGATGAGTGGAATGAAAACTTGACATTTGAAGAAGGTGTCAAGTTACTCGAAAAATGCATGCGAGTGCTTTTGTACCGTGATAGGTCTGCTGTGAACAAGATTCAGATATCTAAAATTACCGAAGAAGGTGCTACTGTTTACCCACCATTCTCTTTGAAAACATACTGGGAGTTCTCGGCCTTCAAGAATCCAACAGTTGGTGCTGAAGGTTCATGGTAG

>MsG0680035574.01.T02

Protein sequence

>MsG0680035574.01.T01

MDTVTESQRTLYPYVTGSSVVAIKYKDGILMAADMGGSYGSTLRYKSVERLKPIGKHSLLGASGEISDFQEIMRYLDELILIDNMWDDGNSLGPKEVHNYLTRVMYNRRNKFNPLWNSLVLGGVKKGQKYLGTVSMIGVNYEDNHVATGLGNHLARPILRDEWNENLTFEEGVKLLEKCMRVLLYRDRSAVNKIQISKITEEGATVYPPFSLKTYWEFSAFKNPTVGAEGSW*

>MsG0680035574.01.T02