AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024840.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024840.01.T01 MTR_5g017680 89.258 391 18 3 1 368 1 390 0.0 690
MsG0580024840.01.T01 MTR_5g017670 83.505 388 37 5 4 368 10 393 0.0 639
MsG0580024840.01.T01 MTR_1g019940 52.094 382 155 4 1 356 9 388 7.11e-137 397
MsG0580024840.01.T01 MTR_1g019940 52.094 382 153 5 1 356 9 386 1.00e-134 391
MsG0580024840.01.T01 MTR_3g101410 51.752 371 154 3 8 356 18 385 3.52e-126 369
MsG0580024840.01.T01 MTR_3g101400 51.482 371 155 3 8 356 18 385 1.78e-125 367
MsG0580024840.01.T01 MTR_1g019940 48.881 268 115 1 111 356 2 269 4.23e-85 260
MsG0580024840.01.T01 MTR_8g007140 41.240 371 167 8 8 356 31 372 2.15e-71 229
MsG0580024840.01.T01 MTR_8g007040 38.017 363 184 8 8 346 30 375 1.21e-68 222
MsG0580024840.01.T01 MTR_8g007090 36.927 371 194 8 10 356 32 386 5.28e-66 215
MsG0580024840.01.T01 MTR_8g007095 36.658 371 195 8 10 356 32 386 1.09e-65 214
MsG0580024840.01.T01 MTR_8g007125 39.459 370 186 7 8 356 30 382 1.81e-62 206
MsG0580024840.01.T01 MTR_8g007065 36.986 365 200 8 10 356 32 384 6.34e-60 199
MsG0580024840.01.T01 MTR_1g095165 31.023 303 173 9 10 298 75 355 1.44e-28 116
MsG0580024840.01.T01 MTR_1g072190 28.682 258 162 9 12 264 32 272 6.15e-25 105
MsG0580024840.01.T01 MTR_7g094940 27.344 256 152 8 12 256 32 264 2.82e-19 88.6
MsG0580024840.01.T01 MTR_2g029510 28.701 331 193 15 10 329 69 367 4.84e-18 85.5
MsG0580024840.01.T01 MTR_0368s0010 30.061 163 98 4 136 298 45 191 7.02e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024840.01.T01 AT4G37070 52.830 371 150 3 8 356 17 384 3.18e-137 397
MsG0580024840.01.T01 AT4G37070 52.973 370 149 3 8 355 17 383 9.48e-137 395
MsG0580024840.01.T01 AT4G37070 52.973 370 149 3 8 355 17 383 9.48e-137 395
MsG0580024840.01.T01 AT4G37060 51.662 391 163 4 9 376 18 405 7.72e-135 391
MsG0580024840.01.T01 AT2G26560 54.424 373 133 6 8 350 17 382 3.49e-131 382
MsG0580024840.01.T01 AT4G37050 54.907 377 141 4 8 356 33 408 1.05e-129 379
MsG0580024840.01.T01 AT4G37060 47.867 422 163 5 9 376 18 436 3.77e-128 375
MsG0580024840.01.T01 AT4G37070 50.541 370 147 4 8 355 17 372 1.84e-126 369
MsG0580024840.01.T01 AT5G43590 49.333 375 154 9 8 356 12 376 2.37e-97 295
MsG0580024840.01.T01 AT5G43590 49.333 375 154 9 8 356 79 443 1.26e-96 296
MsG0580024840.01.T01 AT3G54950 30.588 340 199 11 10 329 98 420 1.63e-32 127
MsG0580024840.01.T01 AT2G39220 30.499 341 201 11 10 329 108 433 1.64e-31 125
MsG0580024840.01.T01 AT4G29800 30.662 287 165 10 6 276 117 385 4.82e-25 106
MsG0580024840.01.T01 AT4G29800 30.662 287 165 10 6 276 117 385 4.89e-25 106
MsG0580024840.01.T01 AT3G63200 27.490 251 154 9 12 255 32 261 3.12e-24 103

Find 77 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCAATGGTGCCCTTATC+TGG 0.232624 5:+10573172 None:intergenic
ATGCTAAGATGGTTGCTAAA+TGG 0.247274 5:-10574163 MsG0580024840.01.T01:CDS
TAACAACGTTTGTCAAAGTT+TGG 0.299732 5:+10575025 None:intergenic
ATTTCCTTGGCAGCAAATAA+AGG 0.355627 5:+10575386 None:intergenic
ATAACCGTTCTTAGCATTGA+TGG 0.361031 5:-10575681 MsG0580024840.01.T01:CDS
TGCTAAGATGGTTGCTAAAT+GGG 0.379655 5:-10574162 MsG0580024840.01.T01:CDS
AAAGGACGGTTATTAGCCTT+TGG 0.381212 5:+10575404 None:intergenic
CGGTTATTAGCCTTTGGATT+GGG 0.382860 5:+10575410 None:intergenic
ATTGTTATATCCATAGGAAC+TGG 0.387387 5:-10574212 MsG0580024840.01.T01:CDS
AGAAGTGGGGATAATGGTAT+AGG 0.393621 5:-10574248 MsG0580024840.01.T01:CDS
TTATCCCCACTTCTCCCATC+TGG 0.399340 5:+10574257 None:intergenic
TTTGTTTGATCCAGTTCCTA+TGG 0.403141 5:+10574202 None:intergenic
TTACCTTGAATCTCAACTTC+AGG 0.404380 5:-10575619 MsG0580024840.01.T01:intron
GAAACAGGATGATACATTAC+AGG 0.404402 5:-10573320 MsG0580024840.01.T01:intron
TTACTTTATCAGGAGATAGA+TGG 0.412330 5:-10575515 MsG0580024840.01.T01:intron
CAATGAGGCAGGCAACGATA+TGG 0.424128 5:-10574087 MsG0580024840.01.T01:intron
GATGGGAGAAGTGGGGATAA+TGG 0.430947 5:-10574254 MsG0580024840.01.T01:CDS
CTAAATGGGGTGCTTTGACT+TGG 0.433220 5:-10574148 MsG0580024840.01.T01:CDS
TATGAAACTGTTCCAGATAA+GGG 0.437927 5:-10573184 MsG0580024840.01.T01:CDS
TTTGAATAATCTAGTAAAAG+TGG 0.438859 5:-10573260 MsG0580024840.01.T01:CDS
CCGTCCTTTATTTGCTGCCA+AGG 0.456116 5:-10575390 MsG0580024840.01.T01:CDS
ATTGATGGTGGAGGCGTAAG+AGG 0.459201 5:-10575666 MsG0580024840.01.T01:CDS
TAACCTGAAGTTGAGATTCA+AGG 0.467692 5:+10575616 None:intergenic
GAAGTGGGGATAATGGTATA+GGG 0.474757 5:-10574247 MsG0580024840.01.T01:CDS
ATATGAAACTGTTCCAGATA+AGG 0.477514 5:-10573185 MsG0580024840.01.T01:CDS
CTAGATTGTTTCAATGAGGC+AGG 0.481948 5:-10574098 MsG0580024840.01.T01:CDS
TACACGAGTGAATTTAGATA+CGG 0.484931 5:-10573212 MsG0580024840.01.T01:CDS
GTAAAATGGGACAATTTCCT+TGG 0.485032 5:+10575373 None:intergenic
ACGGTTATTAGCCTTTGGAT+TGG 0.485302 5:+10575409 None:intergenic
TAGTGAGCTGGTAAATAAGT+TGG 0.487210 5:+10574745 None:intergenic
AGGAAACCAGATGGGAGAAG+TGG 0.489397 5:-10574263 MsG0580024840.01.T01:CDS
AAGTGACAAAACATATCATA+AGG 0.498172 5:-10574283 MsG0580024840.01.T01:CDS
AACACAACAAAGGCTTTGAC+AGG 0.510884 5:-10575114 MsG0580024840.01.T01:CDS
AGTTTCTGAACATCAAAAGA+TGG 0.514848 5:+10575000 None:intergenic
AGATAGATGGTGAGGATGCA+AGG 0.519486 5:-10575502 MsG0580024840.01.T01:CDS
TTGAATAATCTAGTAAAAGT+GGG 0.529289 5:-10573259 MsG0580024840.01.T01:CDS
AGAACGGTTATGAAATTCAC+AGG 0.529356 5:+10575693 None:intergenic
TGGTGTGGCTGCTAACAATC+CGG 0.532379 5:-10574665 MsG0580024840.01.T01:intron
TTGACTTGGTTATTCAACAA+TGG 0.542109 5:-10574134 MsG0580024840.01.T01:CDS
CCATTGAGGAAGAACTCAAA+AGG 0.542620 5:-10573159 MsG0580024840.01.T01:intron
GGCGTAAGAGGAATCAATCC+TGG 0.544052 5:-10575654 MsG0580024840.01.T01:CDS
TCATTGAAACAATCTAGTAT+TGG 0.550525 5:+10574104 None:intergenic
GGAAACCAGATGGGAGAAGT+GGG 0.551573 5:-10574262 MsG0580024840.01.T01:CDS
GTAATTGCTGGGACAAGTAC+AGG 0.560086 5:-10575461 MsG0580024840.01.T01:CDS
GGGCAAGTACAATGCTAAGA+TGG 0.561989 5:-10574174 MsG0580024840.01.T01:CDS
GAGAGTAGTACATCTAAAGC+TGG 0.566877 5:+10574796 None:intergenic
AGATATTTGCATAGCTACAT+CGG 0.569187 5:-10574773 MsG0580024840.01.T01:CDS
ACACGAGTGAATTTAGATAC+GGG 0.570644 5:-10573211 MsG0580024840.01.T01:CDS
TGGATTGGGAGTTGCTAACA+TGG 0.571178 5:+10575424 None:intergenic
GTTCTTAGCATTGATGGTGG+AGG 0.583471 5:-10575675 MsG0580024840.01.T01:CDS
CAACCTCATCGACGGTGGTG+TGG 0.586722 5:-10574680 MsG0580024840.01.T01:CDS
AATACTAGATTGTTTCAATG+AGG 0.588722 5:-10574102 MsG0580024840.01.T01:CDS
TCCAGATAAGGGCACCATTG+AGG 0.588948 5:-10573173 MsG0580024840.01.T01:CDS
GCCTTTGTTGTGTTTATGAG+CGG 0.591652 5:+10575123 None:intergenic
CCTTGGCAGCAAATAAAGGA+CGG 0.594651 5:+10575390 None:intergenic
AAAAGAAACTACCTGATAGG+AGG 0.601875 5:+10574960 None:intergenic
AAACAGGATGATACATTACA+GGG 0.605874 5:-10573319 MsG0580024840.01.T01:intron
ACATATACAGACCTTTGCTG+TGG 0.609848 5:+10575326 None:intergenic
ACCGTTCTTAGCATTGATGG+TGG 0.616907 5:-10575678 MsG0580024840.01.T01:CDS
ATATCATAAGGAAACCAGAT+GGG 0.624603 5:-10574271 MsG0580024840.01.T01:CDS
CAGCCACACCACCGTCGATG+AGG 0.625199 5:+10574677 None:intergenic
TCCACCATCAATGCTAAGAA+CGG 0.628277 5:+10575677 None:intergenic
TTGACAGGATCTAAGTACAA+TGG 0.630487 5:-10575099 MsG0580024840.01.T01:CDS
AACTGGATCAAACAAAAGTG+AGG 0.631320 5:-10574195 MsG0580024840.01.T01:CDS
ACTGGATCAAACAAAAGTGA+GGG 0.636380 5:-10574194 MsG0580024840.01.T01:CDS
CATATCATAAGGAAACCAGA+TGG 0.638845 5:-10574272 MsG0580024840.01.T01:CDS
AAAGAATACAACCTCATCGA+CGG 0.641779 5:-10574688 MsG0580024840.01.T01:CDS
TTATCAGGAGATAGATGGTG+AGG 0.644937 5:-10575510 MsG0580024840.01.T01:intron
GTTAGCAACTCCCAATCCAA+AGG 0.652029 5:-10575420 MsG0580024840.01.T01:CDS
GCTAAGATGGTTGCTAAATG+GGG 0.667354 5:-10574161 MsG0580024840.01.T01:CDS
AGGTAAGCAAGAATAACACC+AGG 0.678576 5:+10575636 None:intergenic
ACCGCTCATAAACACAACAA+AGG 0.679121 5:-10575124 MsG0580024840.01.T01:CDS
ACAGGATGATACATTACAGG+GGG 0.697116 5:-10573317 MsG0580024840.01.T01:intron
GAAACCAGATGGGAGAAGTG+GGG 0.707369 5:-10574261 MsG0580024840.01.T01:CDS
ATTGCTGGGACAAGTACAGG+TGG 0.708932 5:-10575458 MsG0580024840.01.T01:CDS
GAATACAACCTCATCGACGG+TGG 0.725120 5:-10574685 MsG0580024840.01.T01:CDS
AACAGGATGATACATTACAG+GGG 0.742212 5:-10573318 MsG0580024840.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAA+CGG - Chr5:10575306-10575325 MsG0580024840.01.T01:intron 0.0%
!! AATTAATTACTAAATTTGTA+AGG - Chr5:10574165-10574184 MsG0580024840.01.T01:CDS 10.0%
!!! AAAATCTATTTCTTAATAAA+TGG - Chr5:10575141-10575160 MsG0580024840.01.T01:intron 10.0%
!!! ATATATATATCATTAGTTTT+AGG + Chr5:10575631-10575650 None:intergenic 10.0%
!!! TTAAGAAATAGATTTTATTA+AGG + Chr5:10575137-10575156 None:intergenic 10.0%
!! AAAAATGCATAATATATGAA+AGG + Chr5:10574916-10574935 None:intergenic 15.0%
!! AATATACTATAGATAAGATT+TGG + Chr5:10575263-10575282 None:intergenic 15.0%
!! ATAAAGTATATATGAAAATC+TGG - Chr5:10573429-10573448 MsG0580024840.01.T01:intron 15.0%
!!! TTAAAACAAGTTTTGATATA+TGG - Chr5:10574227-10574246 MsG0580024840.01.T01:CDS 15.0%
!! CTTAATTTACTTGAATTCTA+AGG + Chr5:10574679-10574698 None:intergenic 20.0%
!! TATTAGTCCTAAGTAATTAA+GGG - Chr5:10575590-10575609 MsG0580024840.01.T01:intron 20.0%
!! TTGAATAATCTAGTAAAAGT+GGG - Chr5:10575429-10575448 MsG0580024840.01.T01:CDS 20.0%
!! TTTGAATAATCTAGTAAAAG+TGG - Chr5:10575428-10575447 MsG0580024840.01.T01:CDS 20.0%
!!! ATATCATTAGTTTTAGGATT+TGG + Chr5:10575625-10575644 None:intergenic 20.0%
!!! ATCTTTTCTTCTTTTTAATG+TGG + Chr5:10574096-10574115 None:intergenic 20.0%
!!! ATTATTTTGATGTAATTGCT+GGG - Chr5:10573216-10573235 MsG0580024840.01.T01:CDS 20.0%
!!! GTCTGTATATGTTTAATTTT+TGG - Chr5:10573373-10573392 MsG0580024840.01.T01:intron 20.0%
!!! TATATGTTGATTACTTTATC+AGG - Chr5:10573163-10573182 MsG0580024840.01.T01:CDS 20.0%
!!! TCTATTTCTTAATAAATGGA+TGG - Chr5:10575145-10575164 MsG0580024840.01.T01:intron 20.0%
!!! TCTTTTCTTCTTTTTAATGT+GGG + Chr5:10574095-10574114 None:intergenic 20.0%
!!! TTTAGTTGATATTTGAATTC+CGG + Chr5:10574805-10574824 None:intergenic 20.0%
! AAAAACTCATTTGTCAACTT+AGG + Chr5:10575205-10575224 None:intergenic 25.0%
! AAATCATATGTCAACAATCA+AGG + Chr5:10574359-10574378 None:intergenic 25.0%
! AAATTGTTACAAACTTGCAA+TGG - Chr5:10575337-10575356 MsG0580024840.01.T01:intron 25.0%
! AAGTAAATTAAGCACATGTT+TGG - Chr5:10574687-10574706 MsG0580024840.01.T01:CDS 25.0%
! AAGTGACAAAACATATCATA+AGG - Chr5:10574405-10574424 MsG0580024840.01.T01:intron 25.0%
! AATACTAATATTATCTGCAG+TGG - Chr5:10573532-10573551 MsG0580024840.01.T01:intron 25.0%
! AATACTAGATTGTTTCAATG+AGG - Chr5:10574586-10574605 MsG0580024840.01.T01:intron 25.0%
! AGTATATATGAAAATCTGGT+TGG - Chr5:10573433-10573452 MsG0580024840.01.T01:intron 25.0%
! ATAATCTTCAAAAGTAAGTC+AGG - Chr5:10574203-10574222 MsG0580024840.01.T01:CDS 25.0%
! ATATGATTTATTGCAGAGTA+TGG - Chr5:10574370-10574389 MsG0580024840.01.T01:intron 25.0%
! ATGCATAATATATGAAAGGT+CGG + Chr5:10574912-10574931 None:intergenic 25.0%
! CAAACAAAATAAAATCTAGC+TGG + Chr5:10575239-10575258 None:intergenic 25.0%
! GTATATATGAAAATCTGGTT+GGG - Chr5:10573434-10573453 MsG0580024840.01.T01:intron 25.0%
! GTATTAGTCCTAAGTAATTA+AGG - Chr5:10575589-10575608 MsG0580024840.01.T01:intron 25.0%
! TACGATTATGTCAAATTTAC+CGG - Chr5:10574783-10574802 MsG0580024840.01.T01:CDS 25.0%
! TATATATATGTTTGCATTGC+AGG - Chr5:10575642-10575661 MsG0580024840.01.T01:CDS 25.0%
! TCATTGAAACAATCTAGTAT+TGG + Chr5:10574587-10574606 None:intergenic 25.0%
! TCGAAAAAAAAGATGAACAT+GGG - Chr5:10573968-10573987 MsG0580024840.01.T01:intron 25.0%
! TTATTTGCAGAAGAAATCTT+GGG + Chr5:10575086-10575105 None:intergenic 25.0%
! TTCGAAAAAAAAGATGAACA+TGG - Chr5:10573967-10573986 MsG0580024840.01.T01:intron 25.0%
! TTCTTAATAAATGGATGGAT+TGG - Chr5:10575150-10575169 MsG0580024840.01.T01:intron 25.0%
! TTTATTTGCAGAAGAAATCT+TGG + Chr5:10575087-10575106 None:intergenic 25.0%
!! CAATTTTGTCTATTGAGTAT+TGG - Chr5:10575035-10575054 MsG0580024840.01.T01:CDS 25.0%
!!! CGTTTTATTGTTATATCCAT+AGG - Chr5:10574470-10574489 MsG0580024840.01.T01:intron 25.0%
!!! CTTTTTTCCCTTAATTACTT+AGG + Chr5:10575600-10575619 None:intergenic 25.0%
!!! GACTTACTTTTGAAGATTAT+TGG + Chr5:10574203-10574222 None:intergenic 25.0%
!!! GAGTTTTTTTTCGATATGAT+TGG + Chr5:10574068-10574087 None:intergenic 25.0%
!!! GATTATTTTGATGTAATTGC+TGG - Chr5:10573215-10573234 MsG0580024840.01.T01:CDS 25.0%
AAACAGGATGATACATTACA+GGG - Chr5:10575369-10575388 MsG0580024840.01.T01:CDS 30.0%
AGATATTTGCATAGCTACAT+CGG - Chr5:10573915-10573934 MsG0580024840.01.T01:intron 30.0%
AGTTTCTGAACATCAAAAGA+TGG + Chr5:10573691-10573710 None:intergenic 30.0%
ATATCATAAGGAAACCAGAT+GGG - Chr5:10574417-10574436 MsG0580024840.01.T01:intron 30.0%
ATATGAAACTGTTCCAGATA+AGG - Chr5:10575503-10575522 MsG0580024840.01.T01:CDS 30.0%
ATTGTTATATCCATAGGAAC+TGG - Chr5:10574476-10574495 MsG0580024840.01.T01:intron 30.0%
CAAAAAAGAAACTACCTGAT+AGG + Chr5:10573734-10573753 None:intergenic 30.0%
GACTACTTCTTTCTATTCTA+TGG - Chr5:10574957-10574976 MsG0580024840.01.T01:intron 30.0%
TAACAACGTTTGTCAAAGTT+TGG + Chr5:10573666-10573685 None:intergenic 30.0%
TACACGAGTGAATTTAGATA+CGG - Chr5:10575476-10575495 MsG0580024840.01.T01:CDS 30.0%
TACTAAATTTGTAAGGACCA+GGG - Chr5:10574172-10574191 MsG0580024840.01.T01:CDS 30.0%
TATGAAACTGTTCCAGATAA+GGG - Chr5:10575504-10575523 MsG0580024840.01.T01:CDS 30.0%
TATTGTTAGTGTTTATGTCG+CGG + Chr5:10573513-10573532 None:intergenic 30.0%
TTACTAAATTTGTAAGGACC+AGG - Chr5:10574171-10574190 MsG0580024840.01.T01:CDS 30.0%
TTACTTTATCAGGAGATAGA+TGG - Chr5:10573173-10573192 MsG0580024840.01.T01:CDS 30.0%
!! TTGACTTGGTTATTCAACAA+TGG - Chr5:10574554-10574573 MsG0580024840.01.T01:intron 30.0%
!!! GCAAGTTTTGAAGGTAAAAT+GGG + Chr5:10573331-10573350 None:intergenic 30.0%
!!! TTTTTTTCGAAATAGTGAGC+TGG + Chr5:10573958-10573977 None:intergenic 30.0%
AAAAAGTCCATCCCACAAAA+TGG - Chr5:10575697-10575716 MsG0580024840.01.T01:CDS 35.0%
AAAAGAAACTACCTGATAGG+AGG + Chr5:10573731-10573750 None:intergenic 35.0%
AAAGAATACAACCTCATCGA+CGG - Chr5:10574000-10574019 MsG0580024840.01.T01:intron 35.0%
AACAGGATGATACATTACAG+GGG - Chr5:10575370-10575389 MsG0580024840.01.T01:CDS 35.0%
AACCATTAAAGTCCTTGTCT+TGG + Chr5:10574331-10574350 None:intergenic 35.0%
AACTGGATCAAACAAAAGTG+AGG - Chr5:10574493-10574512 MsG0580024840.01.T01:intron 35.0%
ACACGAGTGAATTTAGATAC+GGG - Chr5:10575477-10575496 MsG0580024840.01.T01:CDS 35.0%
ACCATTAAAGTCCTTGTCTT+GGG + Chr5:10574330-10574349 None:intergenic 35.0%
ACTGGATCAAACAAAAGTGA+GGG - Chr5:10574494-10574513 MsG0580024840.01.T01:intron 35.0%
AGAACGGTTATGAAATTCAC+AGG + Chr5:10572998-10573017 None:intergenic 35.0%
ATATGAAAATCTGGTTGGGA+TGG - Chr5:10573438-10573457 MsG0580024840.01.T01:intron 35.0%
ATGCTAAGATGGTTGCTAAA+TGG - Chr5:10574525-10574544 MsG0580024840.01.T01:intron 35.0%
ATTTCCTTGGCAGCAAATAA+AGG + Chr5:10573305-10573324 None:intergenic 35.0%
CATAATATATGAAAGGTCGG+AGG + Chr5:10574909-10574928 None:intergenic 35.0%
CATATCATAAGGAAACCAGA+TGG - Chr5:10574416-10574435 MsG0580024840.01.T01:intron 35.0%
CTAACAATCCGGTAAAATTC+TGG - Chr5:10574034-10574053 MsG0580024840.01.T01:intron 35.0%
GAAACAGGATGATACATTAC+AGG - Chr5:10575368-10575387 MsG0580024840.01.T01:CDS 35.0%
GGAAGAACTCAAAAGGTAAT+TGG - Chr5:10575536-10575555 MsG0580024840.01.T01:intron 35.0%
GTAAAATGGGACAATTTCCT+TGG + Chr5:10573318-10573337 None:intergenic 35.0%
TAACCTGAAGTTGAGATTCA+AGG + Chr5:10573075-10573094 None:intergenic 35.0%
TACCTGATAGGAGGAAAAAA+TGG + Chr5:10573722-10573741 None:intergenic 35.0%
TAGTGAGCTGGTAAATAAGT+TGG + Chr5:10573946-10573965 None:intergenic 35.0%
TATGAAAATCTGGTTGGGAT+GGG - Chr5:10573439-10573458 MsG0580024840.01.T01:intron 35.0%
TGATAGGAGGAAAAAATGGT+AGG + Chr5:10573718-10573737 None:intergenic 35.0%
TGCTAAGATGGTTGCTAAAT+GGG - Chr5:10574526-10574545 MsG0580024840.01.T01:intron 35.0%
TTACCTTGAATCTCAACTTC+AGG - Chr5:10573069-10573088 MsG0580024840.01.T01:intron 35.0%
TTGACAGGATCTAAGTACAA+TGG - Chr5:10573589-10573608 MsG0580024840.01.T01:intron 35.0%
TTTCTTACGACCTAAACTCA+CGG - Chr5:10574933-10574952 MsG0580024840.01.T01:intron 35.0%
TTTGTTTGATCCAGTTCCTA+TGG + Chr5:10574489-10574508 None:intergenic 35.0%
! ATAACCGTTCTTAGCATTGA+TGG - Chr5:10573007-10573026 MsG0580024840.01.T01:CDS 35.0%
! CTTTGCTGTGGAAAAATAGA+GGG + Chr5:10573353-10573372 None:intergenic 35.0%
!! AAATAGAGGGCAAGTTTTGA+AGG + Chr5:10573340-10573359 None:intergenic 35.0%
!! TCTATTGAGTATTGGTTGTC+TGG - Chr5:10575043-10575062 MsG0580024840.01.T01:CDS 35.0%
!!! GGCAAGTTTTGAAGGTAAAA+TGG + Chr5:10573332-10573351 None:intergenic 35.0%
!!! TACCATTTTTTCCTCCTATC+AGG - Chr5:10573717-10573736 MsG0580024840.01.T01:intron 35.0%
!!! TCAGTTACTTGCCATTTTGT+GGG + Chr5:10575711-10575730 None:intergenic 35.0%
!!! TTTTGTTGTTGTAGTGTCTG+AGG - Chr5:10574877-10574896 MsG0580024840.01.T01:intron 35.0%
!!! TTTTTTATAGGCCCAAGACA+AGG - Chr5:10574316-10574335 MsG0580024840.01.T01:intron 35.0%
ACAGGATGATACATTACAGG+GGG - Chr5:10575371-10575390 MsG0580024840.01.T01:CDS 40.0%
ACATATACAGACCTTTGCTG+TGG + Chr5:10573365-10573384 None:intergenic 40.0%
ACCGCTCATAAACACAACAA+AGG - Chr5:10573564-10573583 MsG0580024840.01.T01:intron 40.0%
AGGTAAGCAAGAATAACACC+AGG + Chr5:10573055-10573074 None:intergenic 40.0%
ATCTTGGGTTCCAAGAAAGA+TGG + Chr5:10575071-10575090 None:intergenic 40.0%
ATGAAAATCTGGTTGGGATG+GGG - Chr5:10573440-10573459 MsG0580024840.01.T01:intron 40.0%
CCATTGAGGAAGAACTCAAA+AGG - Chr5:10575529-10575548 MsG0580024840.01.T01:intron 40.0%
CTAGATTGTTTCAATGAGGC+AGG - Chr5:10574590-10574609 MsG0580024840.01.T01:intron 40.0%
GCTAAGATGGTTGCTAAATG+GGG - Chr5:10574527-10574546 MsG0580024840.01.T01:intron 40.0%
GTAGTTGTCTTGAGACTGAA+GGG + Chr5:10574657-10574676 None:intergenic 40.0%
TCCACCATCAATGCTAAGAA+CGG + Chr5:10573014-10573033 None:intergenic 40.0%
TTATCAGGAGATAGATGGTG+AGG - Chr5:10573178-10573197 MsG0580024840.01.T01:CDS 40.0%
TTCTTACGACCTAAACTCAC+GGG - Chr5:10574934-10574953 MsG0580024840.01.T01:intron 40.0%
TTGTCTGGATCCATCTTTCT+TGG - Chr5:10575058-10575077 MsG0580024840.01.T01:CDS 40.0%
! AACACAACAAAGGCTTTGAC+AGG - Chr5:10573574-10573593 MsG0580024840.01.T01:intron 40.0%
! AGAAGTGGGGATAATGGTAT+AGG - Chr5:10574440-10574459 MsG0580024840.01.T01:intron 40.0%
! CCTTTGCTGTGGAAAAATAG+AGG + Chr5:10573354-10573373 None:intergenic 40.0%
! GAAGTGGGGATAATGGTATA+GGG - Chr5:10574441-10574460 MsG0580024840.01.T01:intron 40.0%
! GAGAGTAGTACATCTAAAGC+TGG + Chr5:10573895-10573914 None:intergenic 40.0%
! GCCTTTGTTGTGTTTATGAG+CGG + Chr5:10573568-10573587 None:intergenic 40.0%
!! AAAGGACGGTTATTAGCCTT+TGG + Chr5:10573287-10573306 None:intergenic 40.0%
!! ACGGTTATTAGCCTTTGGAT+TGG + Chr5:10573282-10573301 None:intergenic 40.0%
!! CCTCTATTTTTCCACAGCAA+AGG - Chr5:10573351-10573370 MsG0580024840.01.T01:intron 40.0%
!! CGGTTATTAGCCTTTGGATT+GGG + Chr5:10573281-10573300 None:intergenic 40.0%
!! GCAATGGTTTTGCTTGAAAC+AGG - Chr5:10575353-10575372 MsG0580024840.01.T01:CDS 40.0%
!!! CCTTTTGAGTTCTTCCTCAA+TGG + Chr5:10575532-10575551 None:intergenic 40.0%
!!! TTACTTGCCATTTTGTGGGA+TGG + Chr5:10575707-10575726 None:intergenic 40.0%
AGATAGATGGTGAGGATGCA+AGG - Chr5:10573186-10573205 MsG0580024840.01.T01:CDS 45.0%
CCTTGGCAGCAAATAAAGGA+CGG + Chr5:10573301-10573320 None:intergenic 45.0%
GAAGATTATTGGATCGACCC+TGG + Chr5:10574192-10574211 None:intergenic 45.0%
GAGACTGAAGGGCAGTAAAA+AGG + Chr5:10574646-10574665 None:intergenic 45.0%
GCCCAAGACAAGGACTTTAA+TGG - Chr5:10574326-10574345 MsG0580024840.01.T01:intron 45.0%
GGGCAAGTACAATGCTAAGA+TGG - Chr5:10574514-10574533 MsG0580024840.01.T01:intron 45.0%
GGTAGTTGTCTTGAGACTGA+AGG + Chr5:10574658-10574677 None:intergenic 45.0%
GTAATTGCTGGGACAAGTAC+AGG - Chr5:10573227-10573246 MsG0580024840.01.T01:CDS 45.0%
GTTAGCAACTCCCAATCCAA+AGG - Chr5:10573268-10573287 MsG0580024840.01.T01:CDS 45.0%
TCTTACGACCTAAACTCACG+GGG - Chr5:10574935-10574954 MsG0580024840.01.T01:intron 45.0%
! CCCTTGAGCCAGAATTTTAC+CGG + Chr5:10574045-10574064 None:intergenic 45.0%
! CTAAATGGGGTGCTTTGACT+TGG - Chr5:10574540-10574559 MsG0580024840.01.T01:intron 45.0%
! TGGATTGGGAGTTGCTAACA+TGG + Chr5:10573267-10573286 None:intergenic 45.0%
!! ACCGTTCTTAGCATTGATGG+TGG - Chr5:10573010-10573029 MsG0580024840.01.T01:CDS 45.0%
!! CCGGTAAAATTCTGGCTCAA+GGG - Chr5:10574042-10574061 MsG0580024840.01.T01:intron 45.0%
!! GTTCTTAGCATTGATGGTGG+AGG - Chr5:10573013-10573032 MsG0580024840.01.T01:CDS 45.0%
!! TCCGGTAAAATTCTGGCTCA+AGG - Chr5:10574041-10574060 MsG0580024840.01.T01:intron 45.0%
!!! TAAAAGAATTTTTTTTTTAT+AGG - Chr5:10574304-10574323 MsG0580024840.01.T01:CDS 5.0%
!!! TTTTTGTTTATAATTAAATA+TGG - Chr5:10573748-10573767 MsG0580024840.01.T01:intron 5.0%
AAAATCTGGTTGGGATGGGG+TGG - Chr5:10573443-10573462 MsG0580024840.01.T01:intron 50.0%
AAATCTGGTTGGGATGGGGT+GGG - Chr5:10573444-10573463 MsG0580024840.01.T01:intron 50.0%
AGGAAACCAGATGGGAGAAG+TGG - Chr5:10574425-10574444 MsG0580024840.01.T01:intron 50.0%
ATTGCTGGGACAAGTACAGG+TGG - Chr5:10573230-10573249 MsG0580024840.01.T01:CDS 50.0%
CAATGAGGCAGGCAACGATA+TGG - Chr5:10574601-10574620 MsG0580024840.01.T01:intron 50.0%
GAAACCAGATGGGAGAAGTG+GGG - Chr5:10574427-10574446 MsG0580024840.01.T01:intron 50.0%
GAAGTAGTCCCCGTGAGTTT+AGG + Chr5:10574946-10574965 None:intergenic 50.0%
GAATACAACCTCATCGACGG+TGG - Chr5:10574003-10574022 MsG0580024840.01.T01:intron 50.0%
GATGGGAGAAGTGGGGATAA+TGG - Chr5:10574434-10574453 MsG0580024840.01.T01:intron 50.0%
GGAAACCAGATGGGAGAAGT+GGG - Chr5:10574426-10574445 MsG0580024840.01.T01:intron 50.0%
GGCGTAAGAGGAATCAATCC+TGG - Chr5:10573034-10573053 MsG0580024840.01.T01:intron 50.0%
TAATGCCTTCACAAGCGTGC+AGG - Chr5:10575174-10575193 MsG0580024840.01.T01:intron 50.0%
TCCTCAATGGTGCCCTTATC+TGG + Chr5:10575519-10575538 None:intergenic 50.0%
TGGGGGGTTTAAGGAGTCTA+GGG - Chr5:10573463-10573482 MsG0580024840.01.T01:intron 50.0%
TGGTGTGGCTGCTAACAATC+CGG - Chr5:10574023-10574042 MsG0580024840.01.T01:intron 50.0%
TTATCCCCACTTCTCCCATC+TGG + Chr5:10574434-10574453 None:intergenic 50.0%
! CCGTCCTTTATTTGCTGCCA+AGG - Chr5:10573298-10573317 MsG0580024840.01.T01:CDS 50.0%
!! ATTGATGGTGGAGGCGTAAG+AGG - Chr5:10573022-10573041 MsG0580024840.01.T01:CDS 50.0%
!! TCCAGATAAGGGCACCATTG+AGG - Chr5:10575515-10575534 MsG0580024840.01.T01:intron 50.0%
AACTGCCTGCACGCTTGTGA+AGG + Chr5:10575182-10575201 None:intergenic 55.0%
AATCTGGTTGGGATGGGGTG+GGG - Chr5:10573445-10573464 MsG0580024840.01.T01:intron 55.0%
GTGGGGGGTTTAAGGAGTCT+AGG - Chr5:10573462-10573481 MsG0580024840.01.T01:intron 55.0%
GTGTTTATGTCGCGGCCATG+CGG + Chr5:10573505-10573524 None:intergenic 55.0%
TCCATCCTCAATCACCCGCA+TGG - Chr5:10573487-10573506 MsG0580024840.01.T01:intron 55.0%
TGTTTATGTCGCGGCCATGC+GGG + Chr5:10573504-10573523 None:intergenic 55.0%
ATCTGGTTGGGATGGGGTGG+GGG - Chr5:10573446-10573465 MsG0580024840.01.T01:intron 60.0%
CAACCTCATCGACGGTGGTG+TGG - Chr5:10574008-10574027 MsG0580024840.01.T01:intron 60.0%
GCCATGCGGGTGATTGAGGA+TGG + Chr5:10573491-10573510 None:intergenic 60.0%
CAGCCACACCACCGTCGATG+AGG + Chr5:10574014-10574033 None:intergenic 65.0%
GGGATGGGGTGGGGGGTTTA+AGG - Chr5:10573454-10573473 MsG0580024840.01.T01:intron 65.0%
TCTGGTTGGGATGGGGTGGG+GGG - Chr5:10573447-10573466 MsG0580024840.01.T01:intron 65.0%
CGCGGCCATGCGGGTGATTG+AGG + Chr5:10573495-10573514 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 10572980 10575730 10572980 ID=MsG0580024840.01;Name=MsG0580024840.01
Chr5 mRNA 10572980 10575730 10572980 ID=MsG0580024840.01.T01;Parent=MsG0580024840.01;Name=MsG0580024840.01.T01;_AED=0.43;_eAED=0.46;_QI=0|0|0|1|1|1|7|0|376
Chr5 exon 10575620 10575730 10575620 ID=MsG0580024840.01.T01:exon:2018;Parent=MsG0580024840.01.T01
Chr5 exon 10575338 10575525 10575338 ID=MsG0580024840.01.T01:exon:2017;Parent=MsG0580024840.01.T01
Chr5 exon 10574972 10575158 10574972 ID=MsG0580024840.01.T01:exon:2016;Parent=MsG0580024840.01.T01
Chr5 exon 10574666 10574830 10574666 ID=MsG0580024840.01.T01:exon:2015;Parent=MsG0580024840.01.T01
Chr5 exon 10574088 10574324 10574088 ID=MsG0580024840.01.T01:exon:2014;Parent=MsG0580024840.01.T01
Chr5 exon 10573160 10573335 10573160 ID=MsG0580024840.01.T01:exon:2013;Parent=MsG0580024840.01.T01
Chr5 exon 10572980 10573046 10572980 ID=MsG0580024840.01.T01:exon:2012;Parent=MsG0580024840.01.T01
Chr5 CDS 10575620 10575730 10575620 ID=MsG0580024840.01.T01:cds;Parent=MsG0580024840.01.T01
Chr5 CDS 10575338 10575525 10575338 ID=MsG0580024840.01.T01:cds;Parent=MsG0580024840.01.T01
Chr5 CDS 10574972 10575158 10574972 ID=MsG0580024840.01.T01:cds;Parent=MsG0580024840.01.T01
Chr5 CDS 10574666 10574830 10574666 ID=MsG0580024840.01.T01:cds;Parent=MsG0580024840.01.T01
Chr5 CDS 10574088 10574324 10574088 ID=MsG0580024840.01.T01:cds;Parent=MsG0580024840.01.T01
Chr5 CDS 10573160 10573335 10573160 ID=MsG0580024840.01.T01:cds;Parent=MsG0580024840.01.T01
Chr5 CDS 10572980 10573046 10572980 ID=MsG0580024840.01.T01:cds;Parent=MsG0580024840.01.T01
Gene Sequence

>MsG0580024840.01.T01

ATGGAAGCAGTTAAGCCTGTGAATTTCATAACCGTTCTTAGCATTGATGGTGGAGGCGTAAGAGGAATCAATCCTGGTGTTATTCTTGCTTACCTTGAATCTCAACTTCAGGAGATAGATGGTGAGGATGCAAGGATTGCAGATTATTTTGATGTAATTGCTGGGACAAGTACAGGTGGTCTCATTACTACCATGTTAGCAACTCCCAATCCAAAGGCTAATAACCGTCCTTTATTTGCTGCCAAGGAAATTGTCCCATTTTACCTTCAAAACTTGCCCTCTATTTTTCCACAGCAAAGTGGAATATTTGCACCGCTCATAAACACAACAAAGGCTTTGACAGGATCTAAGTACAATGGAGAGTATCTTCACAAGCTGATAAGAAATATGACACAAGATACACTTTTGAGCCAAACTTTGACAAACGTTGTTATTCCATCTTTTGATGTTCAGAAACTTCAACCTACCATTTTTTCCTCCTATCAGATTGAAGCTGAGCCAGCTTTAGATGTACTACTCTCAGATATTTGCATAGCTACATCGGCTGCTCCAACTTATTTACCAGCTCACTATTTCGAAAAAAAAGATGAACATGGGAAAGTCATCAAAGAATACAACCTCATCGACGGTGGTGTGGCTGCTAACAATCCGAGTATGGTTGCAATAAGAGAAGTGACAAAACATATCATAAGGAAACCAGATGGGAGAAGTGGGGATAATGGTATAGGGTATGATCGTTTTATTGTTATATCCATAGGAACTGGATCAAACAAAAGTGAGGGCAAGTACAATGCTAAGATGGTTGCTAAATGGGGTGCTTTGACTTGGTTATTCAACAATGGATCAACTCCAATACTAGATTGTTTCAATGAGGCAGGCAACGATATGGATGATACATTACAGGGGGAATTAGCTTCTGTAGATATATCTACTAAAGAGAATTTGAATAATCTAGTAAAAGTGGGTGAACAGTTACTGAAGAAAAAATTTACACGAGTGAATTTAGATACGGGCATATATGAAACTGTTCCAGATAAGGGCACCATTGAGGAAGAACTCAAAAGGTTTCTAATTTACTCTCAGATATCAGAAAATGCAAAAAGTCCATCCCACAAAATGGCAAGTAACTGA
Protein sequence

>MsG0580024840.01.T01

MEAVKPVNFITVLSIDGGGVRGINPGVILAYLESQLQEIDGEDARIADYFDVIAGTSTGGLITTMLATPNPKANNRPLFAAKEIVPFYLQNLPSIFPQQSGIFAPLINTTKALTGSKYNGEYLHKLIRNMTQDTLLSQTLTNVVIPSFDVQKLQPTIFSSYQIEAEPALDVLLSDICIATSAAPTYLPAHYFEKKDEHGKVIKEYNLIDGGVAANNPSMVAIREVTKHIIRKPDGRSGDNGIGYDRFIVISIGTGSNKSEGKYNAKMVAKWGALTWLFNNGSTPILDCFNEAGNDMDDTLQGELASVDISTKENLNNLVKVGEQLLKKKFTRVNLDTGIYETVPDKGTIEEELKRFLIYSQISENAKSPSHKMASN*