AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001008.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001008.01.T02 MTR_1g028970 93.130 131 9 0 4 134 86 216 1.15e-85 249
MsG0180001008.01.T02 MTR_1g028970 89.130 138 15 0 4 141 86 223 4.56e-84 246
MsG0180001008.01.T02 MTR_1g028960 94.531 128 6 1 4 130 85 212 6.18e-83 243
MsG0180001008.01.T02 MTR_3g090530 67.939 131 41 1 5 134 20 150 4.81e-61 185
MsG0180001008.01.T02 MTR_3g090530 67.939 131 41 1 5 134 85 215 2.91e-60 185
MsG0180001008.01.T02 MTR_3g090530 67.164 134 41 2 5 137 20 151 1.38e-59 181
MsG0180001008.01.T02 MTR_3g090530 67.164 134 41 2 5 137 85 216 6.92e-59 182
MsG0180001008.01.T02 MTR_1g028970 95.000 80 4 0 4 83 86 165 1.07e-49 157
MsG0180001008.01.T02 MTR_3g090530 67.500 80 25 1 5 83 20 99 3.75e-33 113
MsG0180001008.01.T02 MTR_3g090530 67.500 80 25 1 5 83 85 164 1.43e-32 113
MsG0180001008.01.T02 MTR_1g075710 31.200 125 82 1 9 133 74 194 6.68e-21 84.3
MsG0180001008.01.T02 MTR_1g041495 29.600 125 84 1 9 133 74 194 3.48e-19 80.1
MsG0180001008.01.T02 MTR_1g075700 35.556 90 58 0 9 98 70 159 1.03e-16 73.2
MsG0180001008.01.T02 MTR_1g041495 27.612 134 84 2 9 133 74 203 3.54e-16 72.0
MsG0180001008.01.T01 MTR_1g028960 94.928 138 6 1 1 137 86 223 1.10e-90 262
MsG0180001008.01.T01 MTR_1g028970 93.431 137 9 0 1 137 87 223 3.71e-90 261
MsG0180001008.01.T01 MTR_1g028970 94.444 126 7 0 1 126 87 212 2.22e-83 244
MsG0180001008.01.T01 MTR_3g090530 68.116 138 43 1 1 137 20 157 1.38e-65 196
MsG0180001008.01.T01 MTR_3g090530 68.116 138 43 1 1 137 85 222 7.19e-65 197
MsG0180001008.01.T01 MTR_3g090530 68.504 127 39 1 1 126 20 146 3.37e-59 180
MsG0180001008.01.T01 MTR_3g090530 68.504 127 39 1 1 126 85 211 1.59e-58 181
MsG0180001008.01.T01 MTR_1g028970 94.937 79 4 0 1 79 87 165 4.35e-49 155
MsG0180001008.01.T01 MTR_3g090530 67.500 80 25 1 1 79 20 99 3.66e-33 113
MsG0180001008.01.T01 MTR_3g090530 67.500 80 25 1 1 79 85 164 1.41e-32 113
MsG0180001008.01.T01 MTR_1g075710 30.827 133 88 1 5 137 74 202 6.83e-23 89.4
MsG0180001008.01.T01 MTR_1g041495 29.323 133 90 1 5 137 74 202 8.16e-21 84.0
MsG0180001008.01.T01 MTR_1g041495 27.465 142 90 2 5 137 74 211 7.89e-18 76.3
MsG0180001008.01.T01 MTR_1g075700 35.556 90 58 0 5 94 70 159 9.51e-17 73.2
MsG0180001008.01.T03 MTR_1g028970 92.941 85 6 0 1 85 139 223 4.87e-53 164
MsG0180001008.01.T03 MTR_1g028960 93.023 86 5 1 1 85 138 223 3.47e-52 162
MsG0180001008.01.T03 MTR_1g028970 94.595 74 4 0 1 74 139 212 3.28e-46 147
MsG0180001008.01.T03 MTR_3g090530 69.412 85 26 0 1 85 73 157 2.26e-41 133
MsG0180001008.01.T03 MTR_3g090530 69.412 85 26 0 1 85 138 222 1.78e-40 132
MsG0180001008.01.T03 MTR_3g090530 70.270 74 22 0 1 74 73 146 4.54e-35 117
MsG0180001008.01.T03 MTR_3g090530 70.270 74 22 0 1 74 138 211 3.12e-34 116
MsG0180001008.01.T03 MTR_1g075710 30.588 85 55 1 1 85 122 202 2.28e-13 62.4
MsG0180001008.01.T03 MTR_1g028970 96.296 27 1 0 1 27 139 165 9.40e-13 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001008.01.T02 AT1G21360 50.000 126 63 0 8 133 28 153 1.37e-40 133
MsG0180001008.01.T02 AT1G21360 50.000 126 63 0 8 133 90 215 8.63e-40 133
MsG0180001008.01.T02 AT3G21260 53.659 123 57 0 8 130 11 133 8.75e-39 128
MsG0180001008.01.T02 AT3G21260 53.659 123 57 0 8 130 16 138 1.01e-38 128
MsG0180001008.01.T02 AT3G21260 53.659 123 57 0 8 130 100 222 8.99e-38 128
MsG0180001008.01.T02 AT1G21360 57.895 76 32 0 8 83 90 165 3.64e-25 95.5
MsG0180001008.01.T02 AT2G33470 33.065 124 79 1 9 132 74 193 2.40e-20 83.2
MsG0180001008.01.T02 AT2G33470 33.065 124 79 1 9 132 74 193 2.40e-20 83.2
MsG0180001008.01.T01 AT1G21360 50.376 133 66 0 4 136 28 160 1.06e-43 141
MsG0180001008.01.T01 AT3G21260 54.135 133 61 0 4 136 11 143 7.58e-43 138
MsG0180001008.01.T01 AT1G21360 50.376 133 66 0 4 136 90 222 8.70e-43 140
MsG0180001008.01.T01 AT3G21260 54.135 133 61 0 4 136 16 148 1.08e-42 138
MsG0180001008.01.T01 AT3G21260 54.135 133 61 0 4 136 100 232 8.22e-42 139
MsG0180001008.01.T01 AT1G21360 57.895 76 32 0 4 79 90 165 4.87e-25 94.7
MsG0180001008.01.T01 AT2G33470 31.579 133 87 1 5 137 74 202 2.05e-21 85.5
MsG0180001008.01.T01 AT2G33470 31.579 133 87 1 5 137 74 202 2.05e-21 85.5
MsG0180001008.01.T03 AT1G21360 47.619 84 44 0 1 84 77 160 1.89e-23 87.8
MsG0180001008.01.T03 AT1G21360 47.619 84 44 0 1 84 139 222 8.91e-23 87.4
MsG0180001008.01.T03 AT3G21260 52.381 84 40 0 1 84 60 143 1.55e-22 84.7
MsG0180001008.01.T03 AT3G21260 52.381 84 40 0 1 84 65 148 2.07e-22 84.7
MsG0180001008.01.T03 AT3G21260 52.381 84 40 0 1 84 149 232 1.02e-21 85.1
MsG0180001008.01.T03 AT2G33470 31.765 85 54 1 1 85 122 202 3.34e-13 62.0
MsG0180001008.01.T03 AT2G33470 31.765 85 54 1 1 85 122 202 3.34e-13 62.0

Find 19 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGTTGAAATATTGAAAT+TGG 0.200840 1:+14494875 MsG0180001008.01.T01:CDS
TAAGCAGAAAATGCAAATAT+TGG 0.279728 1:+14495554 MsG0180001008.01.T01:CDS
AGTAAGCTTTGTTTATCAAT+TGG 0.388677 1:+14495620 MsG0180001008.01.T01:intron
GCTGCAGTAAGGCCTTTGTT+TGG 0.415497 1:+14494931 MsG0180001008.01.T01:CDS
GGTGGCTCTAAGATTAGTTC+CGG 0.420560 1:+14495476 MsG0180001008.01.T01:intron
AACAGAAGGCAATGCAAAGA+AGG 0.452284 1:+14494902 MsG0180001008.01.T01:CDS
CATTAAAACCATGGCATGGA+TGG 0.453685 1:+14495189 MsG0180001008.01.T01:CDS
ATTGATAAACAAAGCTTACT+AGG 0.462275 1:-14495617 None:intergenic
CAATGGATATCCTCAAGAAA+AGG 0.520025 1:-14495591 None:intergenic
AGGCCTTTGTTTGGCTCACA+AGG 0.523024 1:+14494940 MsG0180001008.01.T01:CDS
ACAGAAGGCAATGCAAAGAA+GGG 0.552195 1:+14494903 MsG0180001008.01.T01:CDS
GAAGGGGTCTAGCTGCAGTA+AGG 0.554936 1:+14494920 MsG0180001008.01.T01:CDS
CAAAGCTTACTAGGATACAA+TGG 0.561451 1:-14495608 None:intergenic
GATGAAATCCATCCATGCCA+TGG 0.583812 1:-14495197 None:intergenic
TTGAAATTGGAAGCAACAGA+AGG 0.584098 1:+14494888 MsG0180001008.01.T01:CDS
ATGAAATTACATTAAAACCA+TGG 0.587709 1:+14495180 MsG0180001008.01.T01:CDS
ATTACATTAAAACCATGGCA+TGG 0.602589 1:+14495185 MsG0180001008.01.T01:CDS
CAGAAGGCAATGCAAAGAAG+GGG 0.645808 1:+14494904 MsG0180001008.01.T01:CDS
TCACCTTGTGAGCCAAACAA+AGG 0.647841 1:-14494943 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTGAAGAATTTATAAAT+AGG + Chr1:14495035-14495054 MsG0180001008.01.T01:intron 10.0%
!! TTTAGTTGAAATATTGAAAT+TGG + Chr1:14494875-14494894 MsG0180001008.01.T01:CDS 15.0%
!!! ATTAGAGAAGTATTATTATT+AGG - Chr1:14494992-14495011 None:intergenic 15.0%
!! AGGTATCATCTTATAATTTA+TGG + Chr1:14495055-14495074 MsG0180001008.01.T01:intron 20.0%
!! ATGAAATTACATTAAAACCA+TGG + Chr1:14495180-14495199 MsG0180001008.01.T01:CDS 20.0%
!! TGAAACGCAATATATATTTA+GGG - Chr1:14495456-14495475 None:intergenic 20.0%
!! TTCAAAAATATACACACATA+AGG - Chr1:14495678-14495697 None:intergenic 20.0%
!! TTTGCAAATTTACATATGAT+AGG + Chr1:14495425-14495444 MsG0180001008.01.T01:intron 20.0%
!!! TTATGTGTGTATATTTTTGA+AGG + Chr1:14495677-14495696 MsG0180001008.01.T01:intron 20.0%
! AAAACAAATACACACACATA+AGG - Chr1:14495705-14495724 None:intergenic 25.0%
! AAAAGATCCTGAAAAGAAAA+TGG + Chr1:14495136-14495155 MsG0180001008.01.T01:CDS 25.0%
! AGGTAAAGTTTGCAATATTT+AGG - Chr1:14495658-14495677 None:intergenic 25.0%
! ATTGATAAACAAAGCTTACT+AGG - Chr1:14495620-14495639 None:intergenic 25.0%
! CTAAATATATATTGCGTTTC+AGG + Chr1:14495455-14495474 MsG0180001008.01.T01:intron 25.0%
! CTGAAACGCAATATATATTT+AGG - Chr1:14495457-14495476 None:intergenic 25.0%
! GGTAAAGTTTGCAATATTTA+GGG - Chr1:14495657-14495676 None:intergenic 25.0%
! TAAGCAGAAAATGCAAATAT+TGG + Chr1:14495554-14495573 MsG0180001008.01.T01:CDS 25.0%
! TAGTATTTCATGCTATTAGA+TGG + Chr1:14495273-14495292 MsG0180001008.01.T01:intron 25.0%
! TTTGCAGAGATTATATAACT+TGG + Chr1:14495722-14495741 MsG0180001008.01.T01:intron 25.0%
!! TCAATGAATGTTTTACTTTC+CGG - Chr1:14495498-14495517 None:intergenic 25.0%
!!! AGTAAGCTTTGTTTATCAAT+TGG + Chr1:14495620-14495639 MsG0180001008.01.T01:intron 25.0%
AATATATATTGCGTTTCAGG+TGG + Chr1:14495458-14495477 MsG0180001008.01.T01:intron 30.0%
ATTACATTAAAACCATGGCA+TGG + Chr1:14495185-14495204 MsG0180001008.01.T01:CDS 30.0%
! ACTTTCTCCATTTTCTTTTC+AGG - Chr1:14495146-14495165 None:intergenic 30.0%
CAAAGCTTACTAGGATACAA+TGG - Chr1:14495611-14495630 None:intergenic 35.0%
CAATGGATATCCTCAAGAAA+AGG - Chr1:14495594-14495613 None:intergenic 35.0%
TAGTATTTGTAACAACGACG+AGG - Chr1:14495114-14495133 None:intergenic 35.0%
TTGAAATTGGAAGCAACAGA+AGG + Chr1:14494888-14494907 MsG0180001008.01.T01:CDS 35.0%
! TTTTCTGCTTAAGCATGTCA+CGG - Chr1:14495545-14495564 None:intergenic 35.0%
AACAGAAGGCAATGCAAAGA+AGG + Chr1:14494902-14494921 MsG0180001008.01.T01:CDS 40.0%
ACAGAAGGCAATGCAAAGAA+GGG + Chr1:14494903-14494922 MsG0180001008.01.T01:CDS 40.0%
CATTAAAACCATGGCATGGA+TGG + Chr1:14495189-14495208 MsG0180001008.01.T01:CDS 40.0%
CAGAAGGCAATGCAAAGAAG+GGG + Chr1:14494904-14494923 MsG0180001008.01.T01:CDS 45.0%
GATGAAATCCATCCATGCCA+TGG - Chr1:14495200-14495219 None:intergenic 45.0%
TCACCTTGTGAGCCAAACAA+AGG - Chr1:14494946-14494965 None:intergenic 45.0%
! TCTGCTTGTGCCTTTTCTTG+AGG + Chr1:14495581-14495600 MsG0180001008.01.T01:CDS 45.0%
!! GGTGGCTCTAAGATTAGTTC+CGG + Chr1:14495476-14495495 MsG0180001008.01.T01:intron 45.0%
!! TGGTGCTGCATATATTCTGC+AGG + Chr1:14495075-14495094 MsG0180001008.01.T01:intron 45.0%
!!! TCTATTTTTATAAAATATTT+AGG + Chr1:14495351-14495370 MsG0180001008.01.T01:intron 5.0%
ACAACGACGAGGTGAAATCC+AGG - Chr1:14495103-14495122 None:intergenic 50.0%
CAACGACGAGGTGAAATCCA+GGG - Chr1:14495102-14495121 None:intergenic 50.0%
GCATATATTCTGCAGGACCC+TGG + Chr1:14495082-14495101 MsG0180001008.01.T01:intron 50.0%
GCTGCAGTAAGGCCTTTGTT+TGG + Chr1:14494931-14494950 MsG0180001008.01.T01:CDS 50.0%
! AGGCCTTTGTTTGGCTCACA+AGG + Chr1:14494940-14494959 MsG0180001008.01.T01:CDS 50.0%
GAAGGGGTCTAGCTGCAGTA+AGG + Chr1:14494920-14494939 MsG0180001008.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 14494852 14495764 14494852 ID=MsG0180001008.01;Name=MsG0180001008.01
Chr1 mRNA 14494864 14495764 14494864 ID=MsG0180001008.01.T01;Parent=MsG0180001008.01;Name=MsG0180001008.01.T01;_AED=0.22;_eAED=0.22;_QI=0|0|0|1|0|0|4|0|137
Chr1 exon 14494864 14494961 14494864 ID=MsG0180001008.01.T01:exon:9867;Parent=MsG0180001008.01.T01
Chr1 CDS 14494864 14494961 14494864 ID=MsG0180001008.01.T01:cds;Parent=MsG0180001008.01.T01
Chr1 CDS 14495097 14495232 14495097 ID=MsG0180001008.01.T01:cds;Parent=MsG0180001008.01.T01
Chr1 CDS 14495477 14495620 14495477 ID=MsG0180001008.01.T01:cds;Parent=MsG0180001008.01.T01
Chr1 CDS 14495729 14495764 14495729 ID=MsG0180001008.01.T01:cds;Parent=MsG0180001008.01.T01
Chr1 mRNA 14494852 14495659 14494852 ID=MsG0180001008.01.T02;Parent=MsG0180001008.01;Name=MsG0180001008.01.T02;_AED=0.21;_eAED=0.22;_QI=0|0|0|1|0|0|3|0|142
Chr1 exon 14494852 14494961 14494852 ID=MsG0180001008.01.T02:exon:9871;Parent=MsG0180001008.01.T02
Chr1 exon 14495477 14495659 14495477 ID=MsG0180001008.01.T02:exon:9872;Parent=MsG0180001008.01.T02
Chr1 CDS 14494852 14494961 14494852 ID=MsG0180001008.01.T02:cds;Parent=MsG0180001008.01.T02
Chr1 CDS 14495097 14495232 14495097 ID=MsG0180001008.01.T02:cds;Parent=MsG0180001008.01.T02
Chr1 CDS 14495477 14495659 14495477 ID=MsG0180001008.01.T02:cds;Parent=MsG0180001008.01.T02
Chr1 mRNA 14495155 14495764 14495155 ID=MsG0180001008.01.T03;Parent=MsG0180001008.01;Name=MsG0180001008.01.T03;_AED=0.33;_eAED=0.33;_QI=0|0|0|1|0|0|3|0|85
Chr1 exon 14495155 14495232 14495155 ID=MsG0180001008.01.T03:exon:9873;Parent=MsG0180001008.01.T03
Chr1 CDS 14495155 14495232 14495155 ID=MsG0180001008.01.T03:cds;Parent=MsG0180001008.01.T03
Chr1 CDS 14495477 14495620 14495477 ID=MsG0180001008.01.T03:cds;Parent=MsG0180001008.01.T03
Chr1 CDS 14495729 14495764 14495729 ID=MsG0180001008.01.T03:cds;Parent=MsG0180001008.01.T03
Gene Sequence

>MsG0180001008.01.T02

ATCAAATCAACAACAAATCTAAATTTAGTTGAAATATTGAAATTGGAAGCAACAGAAGGCAATGCAAAGAAGGGGTCTAGCTGCAGTAAGGCCTTTGTTTGGCTCACAAGGACCCTGGATTTCACCTCGTCGTTGTTACAAATACTATCAAAAGATCCTGAAAAGAAAATGGAGAAAGTAGTTGAAGAATCTTATGAAATTACATTAAAACCATGGCATGGATGGATTTCATCAACTGCTGTTAGAGTGGCTCTAAGATTAGTTCCGGAAAGTAAAACATTCATTGATCTTCTTAAGACTGAAGATGAAGACCGTGACATGCTTAAGCAGAAAATGCAAATATTGGTTTCTCTGCTTGTGCCTTTTCTTGAGGATATCCATTGTATCCTAGTAAGCTTTGTTTATCAATTGGTCAAGAAAACTCCCTAA

>MsG0180001008.01.T01

ACAAATCTAAATTTAGTTGAAATATTGAAATTGGAAGCAACAGAAGGCAATGCAAAGAAGGGGTCTAGCTGCAGTAAGGCCTTTGTTTGGCTCACAAGGACCCTGGATTTCACCTCGTCGTTGTTACAAATACTATCAAAAGATCCTGAAAAGAAAATGGAGAAAGTAGTTGAAGAATCTTATGAAATTACATTAAAACCATGGCATGGATGGATTTCATCAACTGCTGTTAGAGTGGCTCTAAGATTAGTTCCGGAAAGTAAAACATTCATTGATCTTCTTAAGACTGAAGATGAAGACCGTGACATGCTTAAGCAGAAAATGCAAATATTGGTTTCTCTGCTTGTGCCTTTTCTTGAGGATATCCATTGTATCCTAAGATTATATAACTTGGACAAGCTGAAATCAACATGA

>MsG0180001008.01.T03

ATGGAGAAAGTAGTTGAAGAATCTTATGAAATTACATTAAAACCATGGCATGGATGGATTTCATCAACTGCTGTTAGAGTGGCTCTAAGATTAGTTCCGGAAAGTAAAACATTCATTGATCTTCTTAAGACTGAAGATGAAGACCGTGACATGCTTAAGCAGAAAATGCAAATATTGGTTTCTCTGCTTGTGCCTTTTCTTGAGGATATCCATTGTATCCTAAGATTATATAACTTGGACAAGCTGAAATCAACATGA

Protein sequence

>MsG0180001008.01.T02

IKSTTNLNLVEILKLEATEGNAKKGSSCSKAFVWLTRTLDFTSSLLQILSKDPEKKMEKVVEESYEITLKPWHGWISSTAVRVALRLVPESKTFIDLLKTEDEDRDMLKQKMQILVSLLVPFLEDIHCILVSFVYQLVKKTP*

>MsG0180001008.01.T01

TNLNLVEILKLEATEGNAKKGSSCSKAFVWLTRTLDFTSSLLQILSKDPEKKMEKVVEESYEITLKPWHGWISSTAVRVALRLVPESKTFIDLLKTEDEDRDMLKQKMQILVSLLVPFLEDIHCILRLYNLDKLKST*

>MsG0180001008.01.T03

MEKVVEESYEITLKPWHGWISSTAVRVALRLVPESKTFIDLLKTEDEDRDMLKQKMQILVSLLVPFLEDIHCILRLYNLDKLKST*