Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039949.01.T01 | XP_003624333.2 | 95.349 | 688 | 32 | 0 | 1 | 688 | 165 | 852 | 0 | 1333 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048509.01 | MsG0780039949.01 | 0.806000 | 1.017058e-49 | 5.459289e-47 |
| MsG0180001008.01 | MsG0780039949.01 | 0.810825 | 9.505841e-51 | 5.789712e-48 |
| MsG0180001499.01 | MsG0780039949.01 | 0.812911 | 3.339799e-51 | 2.150103e-48 |
| MsG0180001513.01 | MsG0780039949.01 | 0.817019 | 4.096338e-52 | 2.949301e-49 |
| MsG0180001691.01 | MsG0780039949.01 | 0.804326 | 2.279962e-49 | 1.172152e-46 |
| MsG0180003375.01 | MsG0780039949.01 | 0.810861 | 9.337999e-51 | 5.692953e-48 |
| MsG0180004942.01 | MsG0780039949.01 | 0.802432 | 5.625024e-49 | 2.755096e-46 |
| MsG0180005356.01 | MsG0780039949.01 | 0.805335 | 1.402692e-49 | 7.401341e-47 |
| MsG0280006559.01 | MsG0780039949.01 | 0.800518 | 1.388358e-48 | 6.477642e-46 |
| MsG0280006829.01 | MsG0780039949.01 | 0.815604 | 8.492637e-52 | 5.882208e-49 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039949.01.T01 | MTR_7g082110 | 95.349 | 688 | 32 | 0 | 1 | 688 | 165 | 852 | 0.0 | 1333 |
| MsG0780039949.01.T01 | MTR_4g044393 | 80.299 | 670 | 126 | 5 | 1 | 669 | 167 | 831 | 0.0 | 1101 |
| MsG0780039949.01.T01 | MTR_7g082300 | 62.607 | 698 | 240 | 7 | 1 | 682 | 168 | 860 | 0.0 | 815 |
| MsG0780039949.01.T01 | MTR_7g082280 | 52.941 | 680 | 201 | 13 | 1 | 677 | 170 | 733 | 0.0 | 621 |
| MsG0780039949.01.T01 | MTR_7g060570 | 52.640 | 663 | 265 | 14 | 15 | 663 | 1 | 628 | 0.0 | 608 |
| MsG0780039949.01.T01 | MTR_7g082470 | 71.960 | 403 | 108 | 4 | 286 | 687 | 252 | 650 | 0.0 | 588 |
| MsG0780039949.01.T01 | MTR_7g082510 | 59.839 | 498 | 176 | 6 | 212 | 687 | 166 | 661 | 0.0 | 574 |
| MsG0780039949.01.T01 | MTR_7g082430 | 73.316 | 386 | 98 | 3 | 301 | 683 | 243 | 626 | 0.0 | 572 |
| MsG0780039949.01.T01 | MTR_1g115485 | 70.792 | 404 | 107 | 5 | 286 | 685 | 278 | 674 | 0.0 | 571 |
| MsG0780039949.01.T01 | MTR_7g082460 | 69.287 | 407 | 119 | 3 | 281 | 687 | 265 | 665 | 0.0 | 563 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039949.01.T01 | AT1G70250 | 55.376 | 372 | 141 | 8 | 296 | 662 | 401 | 752 | 2.02e-129 | 402 |
| MsG0780039949.01.T01 | AT5G38280 | 59.062 | 320 | 123 | 4 | 358 | 673 | 319 | 634 | 2.82e-127 | 392 |
| MsG0780039949.01.T01 | AT5G38280 | 59.062 | 320 | 123 | 4 | 358 | 673 | 319 | 634 | 6.88e-127 | 391 |
| MsG0780039949.01.T01 | AT4G18250 | 52.507 | 379 | 154 | 8 | 289 | 659 | 288 | 648 | 2.04e-126 | 390 |
| MsG0780039949.01.T01 | AT1G66910 | 51.706 | 381 | 172 | 6 | 303 | 677 | 277 | 651 | 8.64e-122 | 378 |
| MsG0780039949.01.T01 | AT1G66910 | 51.157 | 389 | 178 | 6 | 295 | 677 | 272 | 654 | 1.31e-121 | 377 |
| MsG0780039949.01.T01 | AT1G66920 | 55.523 | 344 | 137 | 8 | 325 | 659 | 258 | 594 | 3.21e-121 | 375 |
| MsG0780039949.01.T01 | AT5G38250 | 50.633 | 395 | 159 | 7 | 295 | 686 | 258 | 619 | 3.22e-121 | 375 |
| MsG0780039949.01.T01 | AT2G01820 | 36.134 | 714 | 370 | 21 | 1 | 644 | 166 | 863 | 4.77e-121 | 384 |
| MsG0780039949.01.T01 | AT1G66920 | 55.523 | 344 | 137 | 8 | 325 | 659 | 250 | 586 | 6.07e-121 | 374 |
Find 137 sgRNAs with CRISPR-Local
Find 159 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTCGTTTCGAGATAAGT+TGG | 0.465213 | 7:+70971487 | MsG0780039949.01.T01:CDS |
| AAACTGGCGAATTCTTGGAA+AGG | 0.606001 | 7:+70970671 | MsG0780039949.01.T01:CDS |
| AAAGTAATAAGTGAGTGCAA+GGG | 0.609209 | 7:+70971572 | MsG0780039949.01.T01:CDS |
| AAAGTAATAGAAATGCTACA+AGG | 0.661359 | 7:+70972295 | MsG0780039949.01.T01:CDS |
| AAATGCTCGACTGAATACCT+CGG | 0.653595 | 7:-70972008 | None:intergenic |
| AACAATCAGAACGACATGTT+CGG | 0.412825 | 7:+70970287 | MsG0780039949.01.T01:CDS |
| AACCATTCAGGGGTATCATT+TGG | 0.395007 | 7:+70971278 | MsG0780039949.01.T01:intron |
| AACGACATGTTCGGGTTTAC+AGG | 0.226925 | 7:+70970296 | MsG0780039949.01.T01:CDS |
| AACTCGTTTCGAGATAAGTT+GGG | 0.504672 | 7:+70971488 | MsG0780039949.01.T01:CDS |
| AACTGGCGAATTCTTGGAAA+GGG | 0.491401 | 7:+70970672 | MsG0780039949.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGTAATAAGTGAGTGCAA+GGG | + | Chr7:70971572-70971591 | MsG0780039949.01.T01:CDS | 30.0% | |
| AAGTCTGATGTATACAGTTA+TGG | + | Chr7:70972046-70972065 | MsG0780039949.01.T01:CDS | 30.0% | |
| AGATCCTTATATATCCAATC+CGG | - | Chr7:70972148-70972167 | None:intergenic | 30.0% | |
| ATAGTTATGCTGAAGTGAAA+AGG | + | Chr7:70971459-70971478 | MsG0780039949.01.T01:CDS | 30.0% | |
| ATATATAAGGATCTTGAGCA+AGG | + | Chr7:70972154-70972173 | MsG0780039949.01.T01:CDS | 30.0% | |
| CTATTGAAATCAGGTTCATT+AGG | - | Chr7:70970569-70970588 | None:intergenic | 30.0% | |
| CTTATAAAGAACAAGTTCAC+TGG | + | Chr7:70970365-70970384 | MsG0780039949.01.T01:CDS | 30.0% | |
| GAACTTATCTAATGATCCAT+TGG | - | Chr7:70971720-70971739 | None:intergenic | 30.0% | |
| GATGATTGTTTGTAAACGTA+AGG | + | Chr7:70971325-70971344 | MsG0780039949.01.T01:CDS | 30.0% | |
| GATTTGAGGAACTTGTATTT+AGG | + | Chr7:70970812-70970831 | MsG0780039949.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 70970137 | 70972447 | 70970137 | ID=MsG0780039949.01;Name=MsG0780039949.01 |
| Chr7 | mRNA | 70970137 | 70972447 | 70970137 | ID=MsG0780039949.01.T01;Parent=MsG0780039949.01;Name=MsG0780039949.01.T01;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|688 |
| Chr7 | exon | 70970137 | 70971043 | 70970137 | ID=MsG0780039949.01.T01:exon:13601;Parent=MsG0780039949.01.T01 |
| Chr7 | exon | 70971288 | 70972447 | 70971288 | ID=MsG0780039949.01.T01:exon:13602;Parent=MsG0780039949.01.T01 |
| Chr7 | CDS | 70970137 | 70971043 | 70970137 | ID=MsG0780039949.01.T01:cds;Parent=MsG0780039949.01.T01 |
| Chr7 | CDS | 70971288 | 70972447 | 70971288 | ID=MsG0780039949.01.T01:cds;Parent=MsG0780039949.01.T01 |
| Gene Sequence |
>MsG0780039949.01.T01 ATGGACCTTGGAAGTACCAATCTCGAGGGTTCCCTGCCGGATATATTTGATTCTTTAGTCAGTTTGCAGGAGCTTCGTCTCTCATATAACAATCTCACAGGGGATTTGCCCAAGTCTTTCTCAGGATCTGGGATTAAGAACATGTGGCTCAACAATCAGAACGACATGTTCGGGTTTACAGGTTCTATTGACGTGCTTGCTTCAATGACTCACGTGGCTCAAGTTTGGCTTATAAAGAACAAGTTCACTGGTCAAATTCCTGATTTGTCCAAGTGTACCAATTTATTTGATCTACAGCTTCGGGATAATCAGTTAACAGGTGTTGTTCCGCCTTCTTTAATGGTCCTGTCTAGTTTGCAGAACATTAGCTTGGACAACAATCAATTGCAGGGCCCTTTTCCTTCTTTTGGGAAAGGTGTACGGTTCAGTCCTAATGAACCTGATTTCAATAGCTTTTGTCGAAACACTTCTGGACCTTGCGATCCACGAGTCACTAATTTGCTCCATATTGCCGGAGATTTTGGGTATCCCCTTAAACTGGCGAATTCTTGGAAAGGGAACAATCCTTGTCAAAACTGGAGATTCATTGTGTGTTCATCGGAGAAGATTATTACAATAAACTTAGCAAAACAGAATTTGAACGGAACCATTTCACCTGCATTTGCAAATTTAACTGATTTGAGGAACTTGTATTTAGGTGATAACAATTTGACTGGCTCAATACCTGAGAGCTTGACAAGTTTGGCACATCTTCAGATTTTGGATGTATCTAACAACAATCTATCTGGAGAGGTTCCTACATTCTCATCTATGCTGAGATTCGATTCAACTGGTAATGCTTTGCTTGGACTTGGAAGTTCATCTCAAAAGTCTACTTCTTCTTTGCTATTGCTGGCTTGGATTTTAGGGGTATCATTTGGTGTAGGAGCTGTACTATTTATTGCGATGATTGTTTGTAAACGTAAGGGATATCTAAGTTTAGTACGAACGAGGATCTTCAAGAAAACAAGAATATCTATTGACCAAGATCATATTGAGGATTTCATAAAAAGATATAATTTGTCAGTGCCGAAGCGGTATAGTTATGCTGAAGTGAAAAGGTTCACAAACTCGTTTCGAGATAAGTTGGGTCAAGGAGGATATGGTGTTGTGTACAAAGCCAGTTTACCTGATGGTCGTCATGTTGCAGTGAAAGTAATAAGTGAGTGCAAGGGAGACGGAGAAGAATTTATAAATGAAGTTGCAAGCATAAGTAAAACATCACATGTGAACATTGTCTCACTTTTGGGTTTTTGTTATGAAAAGAACAAAAGTGCATTGATATATGAATTCATGTCCAATGGATCATTAGATAAGTTCATATATAGAAGTGGATTTCCAAATGCTATTTGTGATTTGGATTGGAACACATTGTTCCATATTGCAATTAGCATTGCTAGAGGACTAGAGTACTTACATCAAGGTTGTATTTCAAGAATTTTGCATCTTGATATCAAACCTCAAAACATTCTTTTAGATGAAGATTTTTGTCCAAAAATATCTGATTTTGGTCTAGCTAAAATATGTCAAAAGAAAGAGAGTGTTGTTTCCCTACTTGGCACAAGAGGAACTATAGGTTACATAGCACCCGAGGTATTCAGTCGAGCATTTGGTGGAGTTTCTTCTAAGTCTGATGTATACAGTTATGGCATGTTGACTCTTGAAATAACTGGAGAAAGAAAGAGTCGTGATACTAGAGGGTCTGATATGACTGAAATGTACTTTCCGGATTGGATATATAAGGATCTTGAGCAAGGTAATACTCTTTTAAATAATTTAACAATCTCTGAAGAAGAAAATGATATAGTAAAGAAGATTACTATGGTGAGTCTCTGGTGCATTCAAACGAATCCATTAGATAGACCATCAATGAGTAAAGTAATAGAAATGCTACAAGGACCACTTCATTCAATACCGTATCCTCCAAAGCCCGTCTTATTTTCTCCAGAAAGGTTTTCATTACAAATGTTAGATACGTCTTCTAGTAATCTTTATGACTCAAATTCAATTATAATAGAGAAAGAATAA |
| Protein sequence |
>MsG0780039949.01.T01 MDLGSTNLEGSLPDIFDSLVSLQELRLSYNNLTGDLPKSFSGSGIKNMWLNNQNDMFGFTGSIDVLASMTHVAQVWLIKNKFTGQIPDLSKCTNLFDLQLRDNQLTGVVPPSLMVLSSLQNISLDNNQLQGPFPSFGKGVRFSPNEPDFNSFCRNTSGPCDPRVTNLLHIAGDFGYPLKLANSWKGNNPCQNWRFIVCSSEKIITINLAKQNLNGTISPAFANLTDLRNLYLGDNNLTGSIPESLTSLAHLQILDVSNNNLSGEVPTFSSMLRFDSTGNALLGLGSSSQKSTSSLLLLAWILGVSFGVGAVLFIAMIVCKRKGYLSLVRTRIFKKTRISIDQDHIEDFIKRYNLSVPKRYSYAEVKRFTNSFRDKLGQGGYGVVYKASLPDGRHVAVKVISECKGDGEEFINEVASISKTSHVNIVSLLGFCYEKNKSALIYEFMSNGSLDKFIYRSGFPNAICDLDWNTLFHIAISIARGLEYLHQGCISRILHLDIKPQNILLDEDFCPKISDFGLAKICQKKESVVSLLGTRGTIGYIAPEVFSRAFGGVSSKSDVYSYGMLTLEITGERKSRDTRGSDMTEMYFPDWIYKDLEQGNTLLNNLTISEEENDIVKKITMVSLWCIQTNPLDRPSMSKVIEMLQGPLHSIPYPPKPVLFSPERFSLQMLDTSSSNLYDSNSIIIEKE* |
