Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026801.01.T01 | MCI20418.1 | 98.611 | 72 | 1 | 0 | 1 | 72 | 16 | 87 | 3.87E-45 | 149 |
| MsG0580026801.01.T02 | MCI20418.1 | 100 | 37 | 0 | 0 | 1 | 37 | 51 | 87 | 5.22E-18 | 79.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026801.01.T01 | Q9ZS97 | 87.5 | 72 | 9 | 0 | 1 | 72 | 486 | 557 | 1.18E-39 | 139 |
| MsG0580026801.01.T02 | Q9ZS97 | 89.189 | 37 | 4 | 0 | 1 | 37 | 521 | 557 | 9.25E-18 | 76.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026801.01.T02 | A0A392Q8F7 | 100.000 | 37 | 0 | 0 | 1 | 37 | 51 | 87 | 2.49e-18 | 79.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000787.01 | MsG0580026801.01 | 0.810719 | 1.002280e-50 | 6.087341e-48 |
| MsG0180004765.01 | MsG0580026801.01 | 0.802939 | 4.421710e-49 | 2.193727e-46 |
| MsG0180006096.01 | MsG0580026801.01 | 0.802143 | 6.453568e-49 | 3.137524e-46 |
| MsG0280006309.01 | MsG0580026801.01 | 0.817080 | 3.970322e-52 | 2.863129e-49 |
| MsG0280010598.01 | MsG0580026801.01 | 0.800723 | 1.260634e-48 | 5.912352e-46 |
| MsG0280010599.01 | MsG0580026801.01 | 0.814594 | 1.422956e-51 | 9.588189e-49 |
| MsG0280010916.01 | MsG0580026801.01 | 0.802338 | 5.882287e-49 | 2.874210e-46 |
| MsG0380012005.01 | MsG0580026801.01 | 0.800598 | 1.336755e-48 | 6.249671e-46 |
| MsG0480018653.01 | MsG0580026801.01 | 0.808880 | 2.492203e-50 | 1.441508e-47 |
| MsG0480020024.01 | MsG0580026801.01 | 0.812093 | 5.042836e-51 | 3.176516e-48 |
| MsG0480021869.01 | MsG0580026801.01 | 0.804847 | 1.775009e-49 | 9.249526e-47 |
| MsG0480023488.01 | MsG0580026801.01 | 0.801588 | 8.386780e-49 | 4.020307e-46 |
| MsG0380016285.01 | MsG0580026801.01 | 0.810919 | 9.070027e-51 | 5.538473e-48 |
| MsG0380016391.01 | MsG0580026801.01 | 0.802019 | 6.842588e-49 | 3.316221e-46 |
| MsG0380017293.01 | MsG0580026801.01 | 0.810615 | 1.055619e-50 | 6.393218e-48 |
| MsG0580026801.01 | MsG0580029376.01 | 0.805967 | 1.033536e-49 | 5.542958e-47 |
| MsG0580026801.01 | MsG0680030616.01 | 0.807305 | 5.394083e-50 | 2.994141e-47 |
| MsG0580026801.01 | MsG0680035580.01 | 0.800386 | 1.476977e-48 | 6.868188e-46 |
| MsG0580026801.01 | MsG0780036593.01 | 0.809594 | 1.752080e-50 | 1.033006e-47 |
| MsG0580026801.01 | MsG0780038439.01 | 0.802901 | 4.502040e-49 | 2.231525e-46 |
| MsG0580026801.01 | MsG0780039443.01 | 0.801563 | 8.488325e-49 | 4.066492e-46 |
| MsG0580026801.01 | MsG0780041187.01 | 0.807963 | 3.909460e-50 | 2.207525e-47 |
| MsG0580026801.01 | MsG0780041416.01 | 0.804043 | 2.610864e-49 | 1.332598e-46 |
| MsG0580026801.01 | MsG0880042622.01 | 0.804000 | 2.664598e-49 | 1.358553e-46 |
| MsG0580026801.01 | MsG0880043157.01 | 0.801155 | 1.028715e-48 | 4.878012e-46 |
| MsG0580026801.01 | MsG0880045992.01 | 0.808520 | 2.974052e-50 | 1.704179e-47 |
| MsG0580026801.01 | MsG0880047045.01 | 0.803737 | 3.022061e-49 | 1.530391e-46 |
| MsG0580026801.01 | MsG0880047135.01 | 0.805704 | 1.173762e-49 | 6.252939e-47 |
| MsG0280007118.01 | MsG0580026801.01 | 0.805480 | 1.308221e-49 | 6.928638e-47 |
| MsG0280008112.01 | MsG0580026801.01 | 0.821313 | 4.315656e-53 | 3.498761e-50 |
| MsG0280008113.01 | MsG0580026801.01 | 0.823687 | 1.210933e-53 | 1.050185e-50 |
| MsG0280009421.01 | MsG0580026801.01 | 0.825321 | 4.996875e-54 | 4.539635e-51 |
| MsG0280010349.01 | MsG0580026801.01 | 0.809600 | 1.746911e-50 | 1.030139e-47 |
| MsG0280010517.01 | MsG0580026801.01 | 0.818852 | 1.579255e-52 | 1.195776e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026801.01.T01 | MTR_8g019500 | 98.611 | 72 | 1 | 0 | 1 | 72 | 475 | 546 | 1.12e-44 | 150 |
| MsG0580026801.01.T01 | MTR_7g038720 | 97.222 | 72 | 2 | 0 | 1 | 72 | 476 | 547 | 4.41e-44 | 148 |
| MsG0580026801.01.T01 | MTR_7g030030 | 97.222 | 72 | 2 | 0 | 1 | 72 | 476 | 547 | 4.41e-44 | 148 |
| MsG0580026801.01.T02 | MTR_8g019500 | 100.000 | 37 | 0 | 0 | 1 | 37 | 510 | 546 | 1.22e-20 | 82.0 |
| MsG0580026801.01.T02 | MTR_7g038720 | 100.000 | 37 | 0 | 0 | 1 | 37 | 511 | 547 | 1.22e-20 | 82.0 |
| MsG0580026801.01.T02 | MTR_7g030030 | 100.000 | 37 | 0 | 0 | 1 | 37 | 511 | 547 | 1.22e-20 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026801.01.T01 | AT4G04770 | 87.500 | 72 | 9 | 0 | 1 | 72 | 486 | 557 | 1.20e-40 | 139 |
| MsG0580026801.01.T01 | AT5G44316 | 84.722 | 72 | 11 | 0 | 1 | 72 | 399 | 470 | 9.68e-40 | 135 |
| MsG0580026801.01.T02 | AT4G04770 | 89.189 | 37 | 4 | 0 | 1 | 37 | 521 | 557 | 9.43e-19 | 76.6 |
| MsG0580026801.01.T02 | AT5G44316 | 83.784 | 37 | 6 | 0 | 1 | 37 | 434 | 470 | 1.61e-17 | 73.2 |
Find 14 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGAGCTTCCCGATGAATT+TGG | 0.201736 | 5:+45094609 | MsG0580026801.01.T01:CDS |
| GAGGCTAGTACGTCGAAAAT+TGG | 0.384901 | 5:+45094495 | MsG0580026801.01.T01:CDS |
| CTCATGAGCTTGAAGCTTGA+GGG | 0.405460 | 5:+45094648 | MsG0580026801.01.T01:CDS |
| GCAATGGCTGCTATGATCTC+TGG | 0.432036 | 5:+45094567 | MsG0580026801.01.T01:CDS |
| TCATGCTCAATTATTGCTGT+TGG | 0.437855 | 5:-45094474 | None:intergenic |
| ACCTCAGAGCCAAATTCATC+GGG | 0.494987 | 5:-45094618 | None:intergenic |
| CACCTCAGAGCCAAATTCAT+CGG | 0.521852 | 5:-45094619 | None:intergenic |
| ACTCATGAGCTTGAAGCTTG+AGG | 0.525889 | 5:+45094647 | MsG0580026801.01.T01:CDS |
| CAAGCTTCAAGCTCATGAGT+TGG | 0.553479 | 5:-45094644 | None:intergenic |
| AACAGCAATAATTGAGCATG+AGG | 0.589380 | 5:+45094476 | MsG0580026801.01.T01:CDS |
| TCCCGATGAATTTGGCTCTG+AGG | 0.597574 | 5:+45094617 | MsG0580026801.01.T01:CDS |
| TCATGAGCTTGAAGCTTGAG+GGG | 0.600976 | 5:+45094649 | MsG0580026801.01.T01:CDS |
| TTGAAGCTTGAGGGGTCAGT+CGG | 0.603318 | 5:+45094657 | MsG0580026801.01.T01:CDS |
| GAAGCTCGTTGAAGACATCA+CGG | 0.677101 | 5:-45094596 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAGACTATGAAAAAGCAA+TGG | + | Chr5:45094551-45094570 | MsG0580026801.01.T01:CDS | 25.0% |
| !!! | TTATTTTACTTTCAACAGAG+AGG | + | Chr5:45094528-45094547 | MsG0580026801.01.T01:CDS | 25.0% |
| AACAGCAATAATTGAGCATG+AGG | + | Chr5:45094476-45094495 | MsG0580026801.01.T01:CDS | 35.0% | |
| TCATGCTCAATTATTGCTGT+TGG | - | Chr5:45094477-45094496 | None:intergenic | 35.0% | |
| AACGAGCTTCCCGATGAATT+TGG | + | Chr5:45094609-45094628 | MsG0580026801.01.T01:CDS | 45.0% | |
| ACCTCAGAGCCAAATTCATC+GGG | - | Chr5:45094621-45094640 | None:intergenic | 45.0% | |
| ACTCATGAGCTTGAAGCTTG+AGG | + | Chr5:45094647-45094666 | MsG0580026801.01.T01:CDS | 45.0% | |
| CAAGCTTCAAGCTCATGAGT+TGG | - | Chr5:45094647-45094666 | None:intergenic | 45.0% | |
| CACCTCAGAGCCAAATTCAT+CGG | - | Chr5:45094622-45094641 | None:intergenic | 45.0% | |
| CTCATGAGCTTGAAGCTTGA+GGG | + | Chr5:45094648-45094667 | MsG0580026801.01.T01:CDS | 45.0% | |
| GAAGCTCGTTGAAGACATCA+CGG | - | Chr5:45094599-45094618 | None:intergenic | 45.0% | |
| GAGGCTAGTACGTCGAAAAT+TGG | + | Chr5:45094495-45094514 | MsG0580026801.01.T01:CDS | 45.0% | |
| TCATGAGCTTGAAGCTTGAG+GGG | + | Chr5:45094649-45094668 | MsG0580026801.01.T01:CDS | 45.0% | |
| GCAATGGCTGCTATGATCTC+TGG | + | Chr5:45094567-45094586 | MsG0580026801.01.T01:CDS | 50.0% | |
| TTGAAGCTTGAGGGGTCAGT+CGG | + | Chr5:45094657-45094676 | MsG0580026801.01.T01:CDS | 50.0% | |
| !! | TCCCGATGAATTTGGCTCTG+AGG | + | Chr5:45094617-45094636 | MsG0580026801.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 45094465 | 45094683 | 45094465 | ID=MsG0580026801.01;Name=MsG0580026801.01 |
| Chr5 | mRNA | 45094465 | 45094683 | 45094465 | ID=MsG0580026801.01.T01;Parent=MsG0580026801.01;Name=MsG0580026801.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|72 |
| Chr5 | exon | 45094465 | 45094683 | 45094465 | ID=MsG0580026801.01.T01:exon:14093;Parent=MsG0580026801.01.T01 |
| Chr5 | CDS | 45094465 | 45094683 | 45094465 | ID=MsG0580026801.01.T01:cds;Parent=MsG0580026801.01.T01 |
| Chr5 | mRNA | 45094570 | 45094683 | 45094570 | ID=MsG0580026801.01.T02;Parent=MsG0580026801.01;Name=MsG0580026801.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|37 |
| Chr5 | exon | 45094570 | 45094683 | 45094570 | ID=MsG0580026801.01.T02:exon:14094;Parent=MsG0580026801.01.T02 |
| Chr5 | CDS | 45094570 | 45094683 | 45094570 | ID=MsG0580026801.01.T02:cds;Parent=MsG0580026801.01.T02 |
| Gene Sequence |
| Protein sequence |
