AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041187.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041187.01.T01 MTR_7g106250 100.000 92 0 0 1 92 689 780 7.85e-61 197
MsG0780041187.01.T01 MTR_1g057700 68.478 92 29 0 1 92 631 722 1.76e-40 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041187.01.T01 AT3G11670 58.696 92 30 1 1 92 725 808 1.81e-32 118

Find 26 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATTTACCAGCCATAGAT+AGG 0.417490 7:-87838009 None:intergenic
GCAAAATCAAGTAGAGATAA+GGG 0.432974 7:+87837796 MsG0780041187.01.T01:CDS
TGCAAAATCAAGTAGAGATA+AGG 0.439836 7:+87837795 MsG0780041187.01.T01:CDS
ACGTGTCCCGGGTGTTCCTC+CGG 0.492705 7:-87837929 None:intergenic
AGAGATAAGGGAAAGCTCAT+TGG 0.497125 7:+87837808 MsG0780041187.01.T01:CDS
CTTGTCATAAACACGTGTCC+CGG 0.502339 7:-87837941 None:intergenic
TCTGCTAGTTCTGAAAGATT+AGG 0.507342 7:-87837847 None:intergenic
TCAAATCCTGAACAAGGAAA+AGG 0.511053 7:+87837768 MsG0780041187.01.T01:CDS
TTTGCTCACTATTGTCTCAC+AGG 0.521736 7:+87837883 MsG0780041187.01.T01:CDS
TTCCTCCGGTACATAATCTC+AGG 0.522670 7:-87837915 None:intergenic
CAGGTGGAAAGTCCTATCTA+TGG 0.536359 7:+87837997 MsG0780041187.01.T01:CDS
AGATAGGACTTTCCACCTGT+GGG 0.544277 7:-87837993 None:intergenic
TTATGTACCGGAGGAACACC+CGG 0.551313 7:+87837922 MsG0780041187.01.T01:CDS
TGGAAAGTCCTATCTATGGC+TGG 0.556422 7:+87838001 MsG0780041187.01.T01:CDS
TTGTCATAAACACGTGTCCC+GGG 0.569933 7:-87837940 None:intergenic
AATCTTTCAGAACTAGCAGA+TGG 0.598240 7:+87837850 MsG0780041187.01.T01:CDS
TAGATAGGACTTTCCACCTG+TGG 0.599056 7:-87837994 None:intergenic
GCTTGATCAAATCCTGAACA+AGG 0.600933 7:+87837762 MsG0780041187.01.T01:CDS
CTTTCAGAACTAGCAGATGG+AGG 0.605213 7:+87837853 MsG0780041187.01.T01:CDS
TATGTACCGGAGGAACACCC+GGG 0.618590 7:+87837923 MsG0780041187.01.T01:CDS
ATAGGACTTTCCACCTGTGG+GGG 0.640769 7:-87837991 None:intergenic
GAGTTCCTGAGATTATGTAC+CGG 0.654856 7:+87837910 MsG0780041187.01.T01:CDS
TTCCTGAGATTATGTACCGG+AGG 0.656952 7:+87837913 MsG0780041187.01.T01:CDS
TTGCTCACTATTGTCTCACA+GGG 0.666888 7:+87837884 MsG0780041187.01.T01:CDS
GATAGGACTTTCCACCTGTG+GGG 0.669701 7:-87837992 None:intergenic
ATCCTGAACAAGGAAAAGGA+TGG 0.691634 7:+87837772 MsG0780041187.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GCAAAATCAAGTAGAGATAA+GGG + Chr7:87837796-87837815 MsG0780041187.01.T01:CDS 30.0%
TGCAAAATCAAGTAGAGATA+AGG + Chr7:87837795-87837814 MsG0780041187.01.T01:CDS 30.0%
AATCTTTCAGAACTAGCAGA+TGG + Chr7:87837850-87837869 MsG0780041187.01.T01:CDS 35.0%
TCAAATCCTGAACAAGGAAA+AGG + Chr7:87837768-87837787 MsG0780041187.01.T01:CDS 35.0%
!! TCTGCTAGTTCTGAAAGATT+AGG - Chr7:87837850-87837869 None:intergenic 35.0%
AGAGATAAGGGAAAGCTCAT+TGG + Chr7:87837808-87837827 MsG0780041187.01.T01:CDS 40.0%
ATCCTGAACAAGGAAAAGGA+TGG + Chr7:87837772-87837791 MsG0780041187.01.T01:CDS 40.0%
GAGTTCCTGAGATTATGTAC+CGG + Chr7:87837910-87837929 MsG0780041187.01.T01:CDS 40.0%
GCTTGATCAAATCCTGAACA+AGG + Chr7:87837762-87837781 MsG0780041187.01.T01:CDS 40.0%
TTGCTCACTATTGTCTCACA+GGG + Chr7:87837884-87837903 MsG0780041187.01.T01:CDS 40.0%
TTTGCTCACTATTGTCTCAC+AGG + Chr7:87837883-87837902 MsG0780041187.01.T01:CDS 40.0%
AGATAGGACTTTCCACCTGT+GGG - Chr7:87837996-87838015 None:intergenic 45.0%
CAGGTGGAAAGTCCTATCTA+TGG + Chr7:87837997-87838016 MsG0780041187.01.T01:CDS 45.0%
CTTGTCATAAACACGTGTCC+CGG - Chr7:87837944-87837963 None:intergenic 45.0%
CTTTCAGAACTAGCAGATGG+AGG + Chr7:87837853-87837872 MsG0780041187.01.T01:CDS 45.0%
TAGATAGGACTTTCCACCTG+TGG - Chr7:87837997-87838016 None:intergenic 45.0%
TGGAAAGTCCTATCTATGGC+TGG + Chr7:87838001-87838020 MsG0780041187.01.T01:CDS 45.0%
TTCCTCCGGTACATAATCTC+AGG - Chr7:87837918-87837937 None:intergenic 45.0%
TTCCTGAGATTATGTACCGG+AGG + Chr7:87837913-87837932 MsG0780041187.01.T01:CDS 45.0%
TTGTCATAAACACGTGTCCC+GGG - Chr7:87837943-87837962 None:intergenic 45.0%
! CACCATCCTTTTCCTTGTTC+AGG - Chr7:87837777-87837796 None:intergenic 45.0%
ATAGGACTTTCCACCTGTGG+GGG - Chr7:87837994-87838013 None:intergenic 50.0%
GATAGGACTTTCCACCTGTG+GGG - Chr7:87837995-87838014 None:intergenic 50.0%
TTATGTACCGGAGGAACACC+CGG + Chr7:87837922-87837941 MsG0780041187.01.T01:CDS 50.0%
CCTGTGGGGGTAAGAGATGA+AGG - Chr7:87837981-87838000 None:intergenic 55.0%
CCTTCATCTCTTACCCCCAC+AGG + Chr7:87837978-87837997 MsG0780041187.01.T01:CDS 55.0%
TATGTACCGGAGGAACACCC+GGG + Chr7:87837923-87837942 MsG0780041187.01.T01:CDS 55.0%
TCATCTCTTACCCCCACAGG+TGG + Chr7:87837981-87838000 MsG0780041187.01.T01:CDS 55.0%
ACGTGTCCCGGGTGTTCCTC+CGG - Chr7:87837932-87837951 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 87837748 87838026 87837748 ID=MsG0780041187.01;Name=MsG0780041187.01
Chr7 mRNA 87837748 87838026 87837748 ID=MsG0780041187.01.T01;Parent=MsG0780041187.01;Name=MsG0780041187.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|92
Chr7 exon 87837748 87838026 87837748 ID=MsG0780041187.01.T01:exon:39484;Parent=MsG0780041187.01.T01
Chr7 CDS 87837748 87838026 87837748 ID=MsG0780041187.01.T01:cds;Parent=MsG0780041187.01.T01
Gene Sequence

>MsG0780041187.01.T01

ATGGAATATTCTGAGCTTGATCAAATCCTGAACAAGGAAAAGGATGGTGCAAAATCAAGTAGAGATAAGGGAAAGCTCATTGGCAAGTCAGCATCAATGCCTAATCTTTCAGAACTAGCAGATGGAGGCTTAGCGTTTGCTCACTATTGTCTCACAGGGAACGAGTTCCTGAGATTATGTACCGGAGGAACACCCGGGACACGTGTTTATGACAAGCAGCACTGTAAAGACCTTCATCTCTTACCCCCACAGGTGGAAAGTCCTATCTATGGCTGGTAA

Protein sequence

>MsG0780041187.01.T01

MEYSELDQILNKEKDGAKSSRDKGKLIGKSASMPNLSELADGGLAFAHYCLTGNEFLRLCTGGTPGTRVYDKQHCKDLHLLPPQVESPIYGW*