AlfalfaGEDB | MsG0580027651.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027651.01

KEGG: K11254
GO: GO:0000786 GO:0003677 GO:0005634 GO:0006334 GO:0046982
PFAM: PF15511

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027651.01.T01	KAG2660576.1	100	103	0	0	1	103	28	130	9.14E-66	204

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027651.01.T01	P0CG89	100	103	0	0	1	103	1	103	3.05E-67	199

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027651.01.T01	A0A453MZH1	100.000	103	0	0	1	103	31	133	3.54e-66	204

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280011003.01	MsG0580027651.01	0.837100	6.343318e-57	8.154595e-54
MsG0280011013.01	MsG0580027651.01	0.833815	4.293913e-56	5.001440e-53
MsG0280011014.01	MsG0580027651.01	0.825435	4.695925e-54	4.279589e-51
MsG0380014289.01	MsG0580027651.01	0.804460	2.137821e-49	1.102871e-46
MsG0480020879.01	MsG0580027651.01	0.806524	7.887716e-50	4.290657e-47
MsG0580024489.01	MsG0580027651.01	0.810112	1.355287e-50	8.100236e-48
MsG0580027650.01	MsG0580027651.01	0.800675	1.289351e-48	6.039881e-46
MsG0580027651.01	MsG0680031214.01	0.802542	5.340099e-49	2.622759e-46
MsG0580027651.01	MsG0780037240.01	0.807536	4.818478e-50	2.690629e-47
MsG0580027651.01	MsG0780040841.01	0.839133	1.901411e-57	2.601135e-54
MsG0580027651.01	MsG0780041535.01	0.842918	1.927591e-58	2.966557e-55
MsG0580027651.01	MsG0880045145.01	0.818622	1.780141e-52	1.339224e-49
MsG0580027651.01	MsG0880047472.01	0.813532	2.441185e-51	1.598319e-48

PPI

Gene1	Gene2	Type
MsG0780041535.01	MsG0580027651.01	PPI
MsG0880047473.01	MsG0580027651.01	PPI
MsG0380014684.01	MsG0580027651.01	PPI
MsG0780037240.01	MsG0580027651.01	PPI
MsG0880043592.01	MsG0580027651.01	PPI
MsG0380015234.01	MsG0580027651.01	PPI
MsG0880047472.01	MsG0580027651.01	PPI
MsG0880047470.01	MsG0580027651.01	PPI
MsG0580027650.01	MsG0580027651.01	PPI
MsG0880045145.01	MsG0580027651.01	PPI
MsG0480020879.01	MsG0580027651.01	PPI
MsG0780040844.01	MsG0580027651.01	PPI
MsG0580027651.01	MsG0880047472.01	PPI
MsG0380014681.01	MsG0580027651.01	PPI
MsG0580027651.01	MsG0480020879.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027651.01.T01	MTR_7g029510	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_7g099610	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_5g063620	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_4g061827	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_4g061920	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_3g462400	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_3g054380	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_3g462730	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_3g462510	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_3g070920	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_3g061440	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_3g462410	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_2g096100	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_8g038460	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0580027651.01.T01	MTR_4g128150	100.000	102	0	1	102	1	102	1.74e-66	204
MsG0580027651.01.T01	MTR_7g029520	84.000	50	8	27	76	1	50	2.23e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027651.01.T01	AT5G59970	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT5G59690	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT5G59970	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT3G46320	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT3G45930	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT3G53730	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT2G28740	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT1G07660	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT1G07820	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT1G07820	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0580027651.01.T01	AT1G07660	83.495	103	1	1	103	1	86	4.52e-51	155

Find 30 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCAAGGAAGGACCCTTTA+TGG	0.236454	5:-65194880	MsG0580027651.01.T01:CDS
GGAAGGACCCTTTATGGATT+TGG	0.275979	5:-65194874	MsG0580027651.01.T01:CDS
GAAGGACCCTTTATGGATTT+GGG	0.341136	5:-65194873	MsG0580027651.01.T01:CDS
GGCAAAGGAGGCAAGGGTTT+GGG	0.348932	5:-65195144	MsG0580027651.01.T01:CDS
AGGCAAAGGAGGCAAGGGTT+TGG	0.398138	5:-65195145	MsG0580027651.01.T01:CDS
GTTCTTAGAGATAACATTCA+AGG	0.421661	5:-65195093	MsG0580027651.01.T01:CDS
TCGCAAAACGGTGACTGCTA+TGG	0.458269	5:-65194923	MsG0580027651.01.T01:CDS
GGCAAGGGTTTGGGAAAGGG+TGG	0.460528	5:-65195135	MsG0580027651.01.T01:CDS
GGAGGCAAGGGTTTGGGAAA+GGG	0.488628	5:-65195138	MsG0580027651.01.T01:CDS
AGGAGGCAAGGGTTTGGGAA+AGG	0.513898	5:-65195139	MsG0580027651.01.T01:CDS
AGGAAGAGGCAAAGGAGGCA+AGG	0.517093	5:-65195151	MsG0580027651.01.T01:CDS
CCGCGATTCGTCGTCTTGCA+AGG	0.525617	5:-65195059	MsG0580027651.01.T01:CDS
ATATTGCAATCAACAATGTC+AGG	0.526915	5:-65195171	None:intergenic
CTTGCAAGACGACGAATCGC+GGG	0.554223	5:+65195060	None:intergenic
GGAAGAGGCAAAGGAGGCAA+GGG	0.555428	5:-65195150	MsG0580027651.01.T01:CDS
TGAACATGCTCGTCGCAAAA+CGG	0.556730	5:-65194935	MsG0580027651.01.T01:CDS
CCTTGCAAGACGACGAATCG+CGG	0.571292	5:+65195059	None:intergenic
CAATCAACAATGTCAGGAAG+AGG	0.577531	5:-65195165	None:intergenic
AAGGACCCTTTATGGATTTG+GGG	0.592631	5:-65194872	MsG0580027651.01.T01:CDS
ATGTGGTTTACGCGCTCAAG+AGG	0.592981	5:-65194900	MsG0580027651.01.T01:CDS
ACGCGCTCAAGAGGCAAGGA+AGG	0.594884	5:-65194891	MsG0580027651.01.T01:CDS
AACGGTGACTGCTATGGATG+TGG	0.598005	5:-65194917	MsG0580027651.01.T01:CDS
ACAATGTCAGGAAGAGGCAA+AGG	0.598135	5:-65195159	MsG0580027651.01.T01:CDS
ATGTCAGGAAGAGGCAAAGG+AGG	0.604597	5:-65195156	MsG0580027651.01.T01:CDS
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	5:-65195051	MsG0580027651.01.T01:CDS
AGGACCCTTTATGGATTTGG+GGG	0.618946	5:-65194871	MsG0580027651.01.T01:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	5:-65195054	MsG0580027651.01.T01:CDS
GTTTACGCGCTCAAGAGGCA+AGG	0.623143	5:-65194895	MsG0580027651.01.T01:CDS
GGTGGTGTAAAGCGTATCAG+TGG	0.649124	5:-65195033	MsG0580027651.01.T01:CDS
CTCATCTATGAAGAAACTCG+TGG	0.682677	5:-65195009	MsG0580027651.01.T01:CDS

CRISPR-GE

sgRNA_sequence	Strand	Position	Region	GC_content
GTTCTTAGAGATAACATTCA+AGG	-	Chr5:65194930-65194949	MsG0580027651.01.T01:CDS	30.0%
AAGGACCCTTTATGGATTTG+GGG	-	Chr5:65195151-65195170	MsG0580027651.01.T01:CDS	40.0%
CTATCCCCCAAATCCATAAA+GGG	+	Chr5:65195159-65195178	None:intergenic	40.0%
CTCATCTATGAAGAAACTCG+TGG	-	Chr5:65195014-65195033	MsG0580027651.01.T01:CDS	40.0%
GAAGGACCCTTTATGGATTT+GGG	-	Chr5:65195150-65195169	MsG0580027651.01.T01:CDS	40.0%
AGGACCCTTTATGGATTTGG+GGG	-	Chr5:65195152-65195171	MsG0580027651.01.T01:CDS	45.0%
GGAAGGACCCTTTATGGATT+TGG	-	Chr5:65195149-65195168	MsG0580027651.01.T01:CDS	45.0%
TGAACATGCTCGTCGCAAAA+CGG	-	Chr5:65195088-65195107	MsG0580027651.01.T01:CDS	45.0%
AACGGTGACTGCTATGGATG+TGG	-	Chr5:65195106-65195125	MsG0580027651.01.T01:CDS	50.0%
AGGCAAGGAAGGACCCTTTA+TGG	-	Chr5:65195143-65195162	MsG0580027651.01.T01:CDS	50.0%
ATGTCAGGAAGAGGCAAAGG+AGG	-	Chr5:65194867-65194886	MsG0580027651.01.T01:CDS	50.0%
ATGTGGTTTACGCGCTCAAG+AGG	-	Chr5:65195123-65195142	MsG0580027651.01.T01:CDS	50.0%
GGTGGTGTAAAGCGTATCAG+TGG	-	Chr5:65194990-65195009	MsG0580027651.01.T01:CDS	50.0%
TCGCAAAACGGTGACTGCTA+TGG	-	Chr5:65195100-65195119	MsG0580027651.01.T01:CDS	50.0%
AGGAAGAGGCAAAGGAGGCA+AGG	-	Chr5:65194872-65194891	MsG0580027651.01.T01:CDS	55.0%
AGGAGGCAAGGGTTTGGGAA+AGG	-	Chr5:65194884-65194903	MsG0580027651.01.T01:CDS	55.0%
AGGCAAAGGAGGCAAGGGTT+TGG	-	Chr5:65194878-65194897	MsG0580027651.01.T01:CDS	55.0%
ATTCGTCGTCTTGCAAGGCG+TGG	-	Chr5:65194969-65194988	MsG0580027651.01.T01:CDS	55.0%
CCTTGCAAGACGACGAATCG+CGG	+	Chr5:65194967-65194986	None:intergenic	55.0%
CTTGCAAGACGACGAATCGC+GGG	+	Chr5:65194966-65194985	None:intergenic	55.0%
GGAAGAGGCAAAGGAGGCAA+GGG	-	Chr5:65194873-65194892	MsG0580027651.01.T01:CDS	55.0%
GGAGGCAAGGGTTTGGGAAA+GGG	-	Chr5:65194885-65194904	MsG0580027651.01.T01:CDS	55.0%
GGCAAAGGAGGCAAGGGTTT+GGG	-	Chr5:65194879-65194898	MsG0580027651.01.T01:CDS	55.0%
GTTTACGCGCTCAAGAGGCA+AGG	-	Chr5:65195128-65195147	MsG0580027651.01.T01:CDS	55.0%
ACGCGCTCAAGAGGCAAGGA+AGG	-	Chr5:65195132-65195151	MsG0580027651.01.T01:CDS	60.0%
CCGCGATTCGTCGTCTTGCA+AGG	-	Chr5:65194964-65194983	MsG0580027651.01.T01:CDS	60.0%
GGCAAGGGTTTGGGAAAGGG+TGG	-	Chr5:65194888-65194907	MsG0580027651.01.T01:CDS	60.0%
CGTCGTCTTGCAAGGCGTGG+TGG	-	Chr5:65194972-65194991	MsG0580027651.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	65194867	65195178	65194867	ID=MsG0580027651.01;Name=MsG0580027651.01
Chr5	mRNA	65194867	65195178	65194867	ID=MsG0580027651.01.T01;Parent=MsG0580027651.01;Name=MsG0580027651.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|103
Chr5	exon	65194867	65195178	65194867	ID=MsG0580027651.01.T01:exon:25344;Parent=MsG0580027651.01.T01
Chr5	CDS	65194867	65195178	65194867	ID=MsG0580027651.01.T01:cds;Parent=MsG0580027651.01.T01

Gene Sequence	>MsG0580027651.01.T01 ATGTCAGGAAGAGGCAAAGGAGGCAAGGGTTTGGGAAAGGGTGGTGCAAAGCGTCATCGCAAAGTTCTTAGAGATAACATTCAAGGAATAACGAAACCCGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTAAAGCGTATCAGTGGTCTCATCTATGAAGAAACTCGTGGTGTTCTCAAGATATTTCTTGAGAATGTTATTCGTGATGCTGTAACTTACACTGAACATGCTCGTCGCAAAACGGTGACTGCTATGGATGTGGTTTACGCGCTCAAGAGGCAAGGAAGGACCCTTTATGGATTTGGGGGATAG
Protein sequence	>MsG0580027651.01.T01 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*