AlfalfaGEDB | MsG0380014684.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014684.01

KEGG: K11254
GO: GO:0000786 GO:0003677 GO:0005634 GO:0006334 GO:0046982
PFAM: PF15511

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014684.01.T01	KAG2660576.1	100	103	0	0	1	103	28	130	9.14E-66	204

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014684.01.T01	P0CG89	100	103	0	0	1	103	1	103	3.05E-67	199

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014684.01.T01	A0A453MZH1	100.000	103	0	0	1	103	31	133	3.54e-66	204

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380014684.01	MsG0380015234.01	0.884032	2.711148e-71	1.760493e-67
MsG0380014684.01	MsG0480020918.01	0.812716	3.685306e-51	2.360408e-48
MsG0380014684.01	MsG0480020927.01	0.859937	2.926755e-63	7.863542e-60
MsG0380014684.01	MsG0480021015.01	0.847288	1.271182e-59	2.248379e-56
MsG0380014684.01	MsG0480021016.01	0.803234	3.843028e-49	1.921050e-46
MsG0380014684.01	MsG0480021302.01	0.869246	3.575691e-66	1.332551e-62
MsG0380014684.01	MsG0780040620.01	0.815071	1.115323e-51	7.612443e-49
MsG0380014684.01	MsG0780040844.01	0.829032	6.456372e-55	6.529098e-52
MsG0380014684.01	MsG0780041592.01	0.826988	2.004605e-54	1.910011e-51

PPI

Gene1	Gene2	Type
MsG0780037240.01	MsG0380014684.01	PPI
MsG0380014684.01	MsG0380014681.01	PPI
MsG0380014684.01	MsG0880043592.01	PPI
MsG0580027650.01	MsG0380014684.01	PPI
MsG0380014684.01	MsG0580027651.01	PPI
MsG0380014684.01	MsG0880047472.01	PPI
MsG0380014684.01	MsG0780041535.01	PPI
MsG0380014684.01	MsG0380015234.01	PPI
MsG0880045145.01	MsG0380014684.01	PPI
MsG0880047473.01	MsG0380014684.01	PPI
MsG0380014684.01	MsG0880047470.01	PPI
MsG0780040844.01	MsG0380014684.01	PPI
MsG0380014684.01	MsG0480020879.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014684.01.T01	MTR_7g029510	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_7g099610	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_5g063620	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_4g061827	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_4g061920	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_3g462400	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_3g054380	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_3g462730	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_3g462510	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_3g070920	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_3g061440	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_3g462410	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_2g096100	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_8g038460	100.000	103	0	1	103	1	103	2.97e-68	199
MsG0380014684.01.T01	MTR_4g128150	100.000	102	0	1	102	1	102	1.74e-66	204
MsG0380014684.01.T01	MTR_7g029520	84.000	50	8	27	76	1	50	2.23e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014684.01.T01	AT5G59970	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT5G59690	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT5G59970	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT3G46320	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT3G45930	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT3G53730	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT2G28740	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT1G07660	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT1G07820	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT1G07820	100.000	103	0	1	103	1	103	3.11e-68	199
MsG0380014684.01.T01	AT1G07660	83.495	103	1	1	103	1	86	4.52e-51	155

Find 36 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCTTCCTTGTCTCTTCA+AGG	0.367893	3:-58399801	None:intergenic
AGACAAGGAAGAACCCTCTA+TGG	0.396310	3:+58399809	MsG0380014684.01.T01:CDS
GAAGATCTTCAAAACACCTC+TGG	0.475512	3:-58399696	None:intergenic
TTCAACCTCCGAATCCATAG+AGG	0.479629	3:-58399823	None:intergenic
CTTGCCAATCGACGAATAGC+AGG	0.484591	3:-58399629	None:intergenic
GGTAAGGGACTCGGAAAGGG+AGG	0.488695	3:+58399554	MsG0380014684.01.T01:CDS
GGAAGAACCCTCTATGGATT+CGG	0.491141	3:+58399815	MsG0380014684.01.T01:CDS
AGGCCGTGGAAAGGGAGGTA+AGG	0.492819	3:+58399538	MsG0380014684.01.T01:CDS
AGGGAGGTGCAAAGAGGCAT+AGG	0.498985	3:+58399570	MsG0380014684.01.T01:CDS
GGAGGTAAGGGACTCGGAAA+GGG	0.499726	3:+58399551	MsG0380014684.01.T01:CDS
CTCGCGAAAATGTCAGGCCG+TGG	0.510433	3:+58399524	None:intergenic
GTCCCTTACCTCCCTTTCCA+CGG	0.513750	3:-58399541	None:intergenic
AAAATGTCAGGCCGTGGAAA+GGG	0.516096	3:+58399530	None:intergenic
GGGAGGTAAGGGACTCGGAA+AGG	0.518226	3:+58399550	MsG0380014684.01.T01:CDS
CAATCGACGAATAGCAGGCT+TGG	0.531595	3:-58399624	None:intergenic
CAAGCCTGCTATTCGTCGAT+TGG	0.532828	3:+58399625	MsG0380014684.01.T01:CDS
GGAAAGGGAGGTAAGGGACT+CGG	0.535187	3:+58399545	MsG0380014684.01.T01:CDS
GGTTCTTCGTGATAACATCC+AGG	0.535931	3:+58399595	MsG0380014684.01.T01:CDS
TTACATACACTGAGCACGCT+AGG	0.551062	3:+58399744	MsG0380014684.01.T01:CDS
GGCCGTGGAAAGGGAGGTAA+GGG	0.552535	3:+58399539	MsG0380014684.01.T01:CDS
GAAAATGTCAGGCCGTGGAA+AGG	0.558967	3:+58399529	None:intergenic
AGGTGCAAAGAGGCATAGGA+AGG	0.586086	3:+58399574	MsG0380014684.01.T01:CDS
CGTCGATTGGCAAGAAGAGG+TGG	0.593946	3:+58399638	MsG0380014684.01.T01:CDS
TAGCAGGCTTGGTGATACCC+TGG	0.594313	3:-58399613	None:intergenic
GAGGAAGACTGTAACAGCCA+TGG	0.599378	3:+58399766	MsG0380014684.01.T01:CDS
GTCTATGCCTTGAAGAGACA+AGG	0.608946	3:+58399794	MsG0380014684.01.T01:CDS
ATTCGTCGATTGGCAAGAAG+AGG	0.629429	3:+58399635	MsG0380014684.01.T01:CDS
CAAGGCATAGACAACATCCA+TGG	0.629842	3:-58399783	None:intergenic
ATGTCAGGCCGTGGAAAGGG+AGG	0.634999	3:+58399533	MsG0380014684.01.T01:CDS
TCAACCTCCGAATCCATAGA+GGG	0.644865	3:-58399822	None:intergenic
GTTCTTCGTGATAACATCCA+GGG	0.651580	3:+58399596	MsG0380014684.01.T01:CDS
AGAACCCTCTATGGATTCGG+AGG	0.656308	3:+58399818	MsG0380014684.01.T01:CDS
GGTGGTGTCAAGAGAATCAG+CGG	0.676498	3:+58399656	MsG0380014684.01.T01:CDS
CATACACTGAGCACGCTAGG+AGG	0.680607	3:+58399747	MsG0380014684.01.T01:CDS
TCGGAAAGGGAGGTGCAAAG+AGG	0.683014	3:+58399564	MsG0380014684.01.T01:CDS
TTGATCTATGAAGAAACCAG+AGG	0.713224	3:+58399680	MsG0380014684.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGATCTATGAAGAAACCAG+AGG	+	Chr3:58399680-58399699	MsG0380014684.01.T01:CDS	35.0%
!!!	AGGTGTTTTGAAGATCTTCT+TGG	+	Chr3:58399700-58399719	MsG0380014684.01.T01:CDS	35.0%
	GAAGATCTTCAAAACACCTC+TGG	-	Chr3:58399699-58399718	None:intergenic	40.0%
	GTTCTTCGTGATAACATCCA+GGG	+	Chr3:58399596-58399615	MsG0380014684.01.T01:CDS	40.0%
	AGACAAGGAAGAACCCTCTA+TGG	+	Chr3:58399809-58399828	MsG0380014684.01.T01:CDS	45.0%
	CAAGGCATAGACAACATCCA+TGG	-	Chr3:58399786-58399805	None:intergenic	45.0%
	GGAAGAACCCTCTATGGATT+CGG	+	Chr3:58399815-58399834	MsG0380014684.01.T01:CDS	45.0%
	GGTTCTTCCTTGTCTCTTCA+AGG	-	Chr3:58399804-58399823	None:intergenic	45.0%
	GGTTCTTCGTGATAACATCC+AGG	+	Chr3:58399595-58399614	MsG0380014684.01.T01:CDS	45.0%
	GTCTATGCCTTGAAGAGACA+AGG	+	Chr3:58399794-58399813	MsG0380014684.01.T01:CDS	45.0%
	TCAACCTCCGAATCCATAGA+GGG	-	Chr3:58399825-58399844	None:intergenic	45.0%
	TTACATACACTGAGCACGCT+AGG	+	Chr3:58399744-58399763	MsG0380014684.01.T01:CDS	45.0%
!	ATTCGTCGATTGGCAAGAAG+AGG	+	Chr3:58399635-58399654	MsG0380014684.01.T01:CDS	45.0%
	AGAACCCTCTATGGATTCGG+AGG	+	Chr3:58399818-58399837	MsG0380014684.01.T01:CDS	50.0%
	CAAGCCTGCTATTCGTCGAT+TGG	+	Chr3:58399625-58399644	MsG0380014684.01.T01:CDS	50.0%
	CAATCGACGAATAGCAGGCT+TGG	-	Chr3:58399627-58399646	None:intergenic	50.0%
	CTTGCCAATCGACGAATAGC+AGG	-	Chr3:58399632-58399651	None:intergenic	50.0%
	GAGGAAGACTGTAACAGCCA+TGG	+	Chr3:58399766-58399785	MsG0380014684.01.T01:CDS	50.0%
!	AGGTGCAAAGAGGCATAGGA+AGG	+	Chr3:58399574-58399593	MsG0380014684.01.T01:CDS	50.0%
!	GGTGGTGTCAAGAGAATCAG+CGG	+	Chr3:58399656-58399675	MsG0380014684.01.T01:CDS	50.0%
	CATACACTGAGCACGCTAGG+AGG	+	Chr3:58399747-58399766	MsG0380014684.01.T01:CDS	55.0%
	GGAAAGGGAGGTAAGGGACT+CGG	+	Chr3:58399545-58399564	MsG0380014684.01.T01:CDS	55.0%
	GGAGGTAAGGGACTCGGAAA+GGG	+	Chr3:58399551-58399570	MsG0380014684.01.T01:CDS	55.0%
	GTCCCTTACCTCCCTTTCCA+CGG	-	Chr3:58399544-58399563	None:intergenic	55.0%
!	AGGGAGGTGCAAAGAGGCAT+AGG	+	Chr3:58399570-58399589	MsG0380014684.01.T01:CDS	55.0%
!	CGTCGATTGGCAAGAAGAGG+TGG	+	Chr3:58399638-58399657	MsG0380014684.01.T01:CDS	55.0%
!	TCGGAAAGGGAGGTGCAAAG+AGG	+	Chr3:58399564-58399583	MsG0380014684.01.T01:CDS	55.0%
!!	TAGCAGGCTTGGTGATACCC+TGG	-	Chr3:58399616-58399635	None:intergenic	55.0%
	AGGCCGTGGAAAGGGAGGTA+AGG	+	Chr3:58399538-58399557	MsG0380014684.01.T01:CDS	60.0%
	ATGTCAGGCCGTGGAAAGGG+AGG	+	Chr3:58399533-58399552	MsG0380014684.01.T01:CDS	60.0%
	GGCCGTGGAAAGGGAGGTAA+GGG	+	Chr3:58399539-58399558	MsG0380014684.01.T01:CDS	60.0%
	GGGAGGTAAGGGACTCGGAA+AGG	+	Chr3:58399550-58399569	MsG0380014684.01.T01:CDS	60.0%
	GGTAAGGGACTCGGAAAGGG+AGG	+	Chr3:58399554-58399573	MsG0380014684.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	58399533	58399844	58399533	ID=MsG0380014684.01;Name=MsG0380014684.01
Chr3	mRNA	58399533	58399844	58399533	ID=MsG0380014684.01.T01;Parent=MsG0380014684.01;Name=MsG0380014684.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|103
Chr3	exon	58399533	58399844	58399533	ID=MsG0380014684.01.T01:exon:18881;Parent=MsG0380014684.01.T01
Chr3	CDS	58399533	58399844	58399533	ID=MsG0380014684.01.T01:cds;Parent=MsG0380014684.01.T01
Chr3	mRNA	58399785	58399844	58399785	ID=MsG0380014684.01.T02;Parent=MsG0380014684.01;Name=MsG0380014684.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|19
Chr3	exon	58399785	58399844	58399785	ID=MsG0380014684.01.T02:exon:18882;Parent=MsG0380014684.01.T02
Chr3	CDS	58399785	58399844	58399785	ID=MsG0380014684.01.T02:cds;Parent=MsG0380014684.01.T02

Gene Sequence

>MsG0380014684.01.T01

ATGTCAGGCCGTGGAAAGGGAGGTAAGGGACTCGGAAAGGGAGGTGCAAAGAGGCATAGGAAGGTTCTTCGTGATAACATCCAGGGTATCACCAAGCCTGCTATTCGTCGATTGGCAAGAAGAGGTGGTGTCAAGAGAATCAGCGGTTTGATCTATGAAGAAACCAGAGGTGTTTTGAAGATCTTCTTGGAAAACGTCATTCGTGATGCTGTTACATACACTGAGCACGCTAGGAGGAAGACTGTAACAGCCATGGATGTTGTCTATGCCTTGAAGAGACAAGGAAGAACCCTCTATGGATTCGGAGGTTGA

>MsG0380014684.01.T02

ATGGATGTTGTCTATGCCTTGAAGAGACAAGGAAGAACCCTCTATGGATTCGGAGGTTGA

Protein sequence

>MsG0380014684.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*

>MsG0380014684.01.T02

MDVVYALKRQGRTLYGFGG*