Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015234.01.T01 | KAG2660576.1 | 100 | 103 | 0 | 0 | 1 | 103 | 28 | 130 | 9.14E-66 | 204 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015234.01.T01 | P0CG89 | 100 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.05E-67 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015234.01.T01 | A0A453MZH1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 31 | 133 | 3.54e-66 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048781.01 | MsG0380015234.01 | 0.830327 | 3.125810e-55 | 3.282663e-52 |
| MsG0080049140.01 | MsG0380015234.01 | 0.821688 | 3.533812e-53 | 2.895298e-50 |
| MsG0180002583.01 | MsG0380015234.01 | 0.800659 | 1.299465e-48 | 6.084735e-46 |
| MsG0180003796.01 | MsG0380015234.01 | 0.818189 | 2.231327e-52 | 1.658808e-49 |
| MsG0180004126.01 | MsG0380015234.01 | 0.805192 | 1.503303e-49 | 7.903026e-47 |
| MsG0180004475.01 | MsG0380015234.01 | 0.810609 | 1.058585e-50 | 6.410130e-48 |
| MsG0180004972.01 | MsG0380015234.01 | 0.819854 | 9.331075e-53 | 7.264501e-50 |
| MsG0180004995.01 | MsG0380015234.01 | 0.846429 | 2.183932e-59 | 3.758365e-56 |
| MsG0180005048.01 | MsG0380015234.01 | 0.850439 | 1.697448e-60 | 3.325288e-57 |
| MsG0180006133.01 | MsG0380015234.01 | 0.832904 | 7.241036e-56 | 8.206764e-53 |
| MsG0280011003.01 | MsG0380015234.01 | 0.835565 | 1.558113e-56 | 1.912477e-53 |
| MsG0280011013.01 | MsG0380015234.01 | 0.829674 | 4.511592e-55 | 4.649642e-52 |
| MsG0280011154.01 | MsG0380015234.01 | 0.834343 | 3.166308e-56 | 3.746975e-53 |
| MsG0380014289.01 | MsG0380015234.01 | 0.849921 | 2.370681e-60 | 4.566098e-57 |
| MsG0380014387.01 | MsG0380015234.01 | 0.818657 | 1.748152e-52 | 1.316418e-49 |
| MsG0380014684.01 | MsG0380015234.01 | 0.884032 | 2.711148e-71 | 1.760493e-67 |
| MsG0380014703.01 | MsG0380015234.01 | 0.811779 | 5.903482e-51 | 3.687283e-48 |
| MsG0380015058.01 | MsG0380015234.01 | 0.836078 | 1.155296e-56 | 1.440307e-53 |
| MsG0380015234.01 | MsG0380016225.01 | 0.807269 | 5.490617e-50 | 3.044808e-47 |
| MsG0380015234.01 | MsG0380017361.01 | 0.804126 | 2.509358e-49 | 1.283493e-46 |
| MsG0380015234.01 | MsG0480018526.01 | 0.811940 | 5.444570e-51 | 3.415262e-48 |
| MsG0380015234.01 | MsG0480018919.01 | 0.800022 | 1.751409e-48 | 8.069221e-46 |
| MsG0380015234.01 | MsG0480020927.01 | 0.846062 | 2.750295e-59 | 4.676606e-56 |
| MsG0380015234.01 | MsG0480020971.01 | 0.825986 | 3.475312e-54 | 3.217635e-51 |
| MsG0380015234.01 | MsG0480021016.01 | 0.859519 | 3.911362e-63 | 1.035791e-59 |
| MsG0380015234.01 | MsG0480021252.01 | 0.829083 | 6.277395e-55 | 6.357714e-52 |
| MsG0380015234.01 | MsG0480021301.01 | 0.812244 | 4.674040e-51 | 2.956457e-48 |
| MsG0380015234.01 | MsG0480021302.01 | 0.841874 | 3.646579e-58 | 5.431535e-55 |
| MsG0380015234.01 | MsG0480022982.01 | 0.841873 | 3.649081e-58 | 5.435154e-55 |
| MsG0380015234.01 | MsG0480023262.01 | 0.802088 | 6.622350e-49 | 3.215180e-46 |
| MsG0380015234.01 | MsG0480023296.01 | 0.806811 | 6.862037e-50 | 3.760427e-47 |
| MsG0380015234.01 | MsG0580024489.01 | 0.815548 | 8.736769e-52 | 6.041789e-49 |
| MsG0380015234.01 | MsG0580024954.01 | 0.803143 | 4.013387e-49 | 2.001526e-46 |
| MsG0380015234.01 | MsG0580025177.01 | 0.807214 | 5.638733e-50 | 3.122490e-47 |
| MsG0380015234.01 | MsG0580025203.01 | 0.825729 | 3.999352e-54 | 3.675693e-51 |
| MsG0380015234.01 | MsG0580025506.01 | 0.842372 | 2.692436e-58 | 4.074193e-55 |
| MsG0380015234.01 | MsG0580028756.01 | 0.839522 | 1.506829e-57 | 2.086181e-54 |
| MsG0380015234.01 | MsG0580029591.01 | 0.829815 | 4.166974e-55 | 4.312708e-52 |
| MsG0380015234.01 | MsG0680032773.01 | 0.818636 | 1.767233e-52 | 1.330051e-49 |
| MsG0380015234.01 | MsG0780039861.01 | 0.803406 | 3.539288e-49 | 1.777110e-46 |
| MsG0380015234.01 | MsG0780040364.01 | 0.820647 | 6.141481e-53 | 4.887255e-50 |
| MsG0380015234.01 | MsG0780040543.01 | 0.802250 | 6.133080e-49 | 2.989977e-46 |
| MsG0380015234.01 | MsG0780040620.01 | 0.853967 | 1.683164e-61 | 3.701530e-58 |
| MsG0380015234.01 | MsG0780040844.01 | 0.867129 | 1.718601e-65 | 5.933703e-62 |
| MsG0380015234.01 | MsG0780041132.01 | 0.812446 | 4.223335e-51 | 2.686009e-48 |
| MsG0380015234.01 | MsG0780041143.01 | 0.858023 | 1.094652e-62 | 2.755354e-59 |
| MsG0380015234.01 | MsG0780041535.01 | 0.811140 | 8.126741e-51 | 4.991099e-48 |
| MsG0380015234.01 | MsG0780041592.01 | 0.837836 | 4.108554e-57 | 5.403136e-54 |
| MsG0380015234.01 | MsG0880042309.01 | 0.802910 | 4.482915e-49 | 2.222564e-46 |
| MsG0380015234.01 | MsG0880043431.01 | 0.807467 | 4.983955e-50 | 2.778178e-47 |
| MsG0380015234.01 | MsG0880045552.01 | 0.809127 | 2.205886e-50 | 1.284483e-47 |
| MsG0380015234.01 | MsG0880046431.01 | 0.807762 | 4.314544e-50 | 2.423434e-47 |
| MsG0380015234.01 | MsG0880047466.01 | 0.810823 | 9.515319e-51 | 5.795096e-48 |
| MsG0280006540.01 | MsG0380015234.01 | 0.816918 | 4.316569e-52 | 3.099100e-49 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015234.01.T01 | MTR_7g029510 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_7g099610 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_5g063620 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_4g061827 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_4g061920 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_3g462400 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_3g054380 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_3g462730 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_3g462510 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_3g070920 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_3g061440 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_3g462410 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_2g096100 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_8g038460 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.97e-68 | 199 |
| MsG0380015234.01.T01 | MTR_4g128150 | 100.000 | 102 | 0 | 0 | 1 | 102 | 1 | 102 | 1.74e-66 | 204 |
| MsG0380015234.01.T01 | MTR_7g029520 | 84.000 | 50 | 8 | 0 | 27 | 76 | 1 | 50 | 2.23e-21 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015234.01.T01 | AT5G59970 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT5G59690 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT5G59970 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT3G46320 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT3G45930 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT3G53730 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT2G28740 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT1G07660 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT1G07820 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT1G07820 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| MsG0380015234.01.T01 | AT1G07660 | 83.495 | 103 | 0 | 1 | 1 | 103 | 1 | 86 | 4.52e-51 | 155 |
Find 36 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGCCAATCGACGAATTGC+AGG | 0.267346 | 3:+67162624 | None:intergenic |
| GGAAGAACCCTCTATGGATT+TGG | 0.370047 | 3:-67162438 | MsG0380015234.01.T01:CDS |
| AGACAAGGAAGAACCCTCTA+TGG | 0.396310 | 3:-67162444 | MsG0380015234.01.T01:CDS |
| AGGTGTCTTGAAGATCTTCT+TGG | 0.405705 | 3:-67162553 | MsG0380015234.01.T01:CDS |
| GGGAGGTAAGGGACTTGGAA+AGG | 0.449159 | 3:-67162703 | MsG0380015234.01.T01:CDS |
| GGAGGTAAGGGACTTGGAAA+GGG | 0.467307 | 3:-67162702 | MsG0380015234.01.T01:CDS |
| GGAAAGGGAGGTAAGGGACT+TGG | 0.470633 | 3:-67162708 | MsG0380015234.01.T01:CDS |
| TTCAACCTCCAAATCCATAG+AGG | 0.476286 | 3:+67162430 | None:intergenic |
| CTGTTGAAAATGTCAGGTCG+TGG | 0.487154 | 3:-67162729 | None:intergenic |
| CAATCGACGAATTGCAGGCT+TGG | 0.491738 | 3:+67162629 | None:intergenic |
| AGGTCGTGGAAAGGGAGGTA+AGG | 0.492819 | 3:-67162715 | MsG0380015234.01.T01:CDS |
| AGTTCTCCGCGATAACATCC+AGG | 0.504812 | 3:-67162658 | MsG0380015234.01.T01:CDS |
| AGAACCCTCTATGGATTTGG+AGG | 0.508817 | 3:-67162435 | MsG0380015234.01.T01:CDS |
| GAAGATCTTCAAGACACCTC+TGG | 0.510902 | 3:+67162557 | None:intergenic |
| CAAGCCTGCAATTCGTCGAT+TGG | 0.511374 | 3:-67162628 | MsG0380015234.01.T01:CDS |
| TTACATACACTGAGCATGCT+AGG | 0.520723 | 3:-67162509 | MsG0380015234.01.T01:CDS |
| GAGGAAGACGGTGACAGCCA+TGG | 0.546349 | 3:-67162487 | MsG0380015234.01.T01:CDS |
| AGGGAGGTGCAAAGAGGCAT+AGG | 0.547891 | 3:-67162683 | MsG0380015234.01.T01:CDS |
| AAAATGTCAGGTCGTGGAAA+GGG | 0.548959 | 3:-67162723 | MsG0380015234.01.T01:CDS |
| GGTAAGGGACTTGGAAAGGG+AGG | 0.560743 | 3:-67162699 | MsG0380015234.01.T01:CDS |
| GGTCGTGGAAAGGGAGGTAA+GGG | 0.564245 | 3:-67162714 | MsG0380015234.01.T01:CDS |
| GTCTATGCTTTGAAGAGACA+AGG | 0.570048 | 3:-67162459 | MsG0380015234.01.T01:CDS |
| GAAAATGTCAGGTCGTGGAA+AGG | 0.584382 | 3:-67162724 | None:intergenic |
| CGTCGATTGGCGAGAAGAGG+CGG | 0.593362 | 3:-67162615 | MsG0380015234.01.T01:CDS |
| GGCGGTGTTAAGAGAATCAG+TGG | 0.595979 | 3:-67162597 | MsG0380015234.01.T01:CDS |
| ATTCGTCGATTGGCGAGAAG+AGG | 0.604517 | 3:-67162618 | MsG0380015234.01.T01:CDS |
| TTGCAGGCTTGGTGATACCC+TGG | 0.613296 | 3:+67162640 | None:intergenic |
| CAAAGCATAGACAACATCCA+TGG | 0.629842 | 3:+67162470 | None:intergenic |
| TCAACCTCCAAATCCATAGA+GGG | 0.633476 | 3:+67162431 | None:intergenic |
| ATGTCAGGTCGTGGAAAGGG+AGG | 0.643397 | 3:-67162720 | MsG0380015234.01.T01:CDS |
| TGATACCCTGGATGTTATCG+CGG | 0.665173 | 3:+67162652 | None:intergenic |
| TGAGCATGCTAGGAGGAAGA+CGG | 0.671956 | 3:-67162499 | MsG0380015234.01.T01:CDS |
| GTTCTCCGCGATAACATCCA+GGG | 0.672360 | 3:-67162657 | MsG0380015234.01.T01:CDS |
| TTGGAAAGGGAGGTGCAAAG+AGG | 0.683014 | 3:-67162689 | MsG0380015234.01.T01:CDS |
| TTGATCTATGAAGAAACCAG+AGG | 0.704889 | 3:-67162573 | MsG0380015234.01.T01:CDS |
| CATACACTGAGCATGCTAGG+AGG | 0.707859 | 3:-67162506 | MsG0380015234.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGATCTATGAAGAAACCAG+AGG | - | Chr3:67162578-67162597 | MsG0380015234.01.T01:CDS | 35.0% | |
| AGGTGTCTTGAAGATCTTCT+TGG | - | Chr3:67162598-67162617 | MsG0380015234.01.T01:CDS | 40.0% | |
| CAAAGCATAGACAACATCCA+TGG | + | Chr3:67162684-67162703 | None:intergenic | 40.0% | |
| TCAACCTCCAAATCCATAGA+GGG | + | Chr3:67162723-67162742 | None:intergenic | 40.0% | |
| TTACATACACTGAGCATGCT+AGG | - | Chr3:67162642-67162661 | MsG0380015234.01.T01:CDS | 40.0% | |
| !! | GTCTATGCTTTGAAGAGACA+AGG | - | Chr3:67162692-67162711 | MsG0380015234.01.T01:CDS | 40.0% |
| AGAACCCTCTATGGATTTGG+AGG | - | Chr3:67162716-67162735 | MsG0380015234.01.T01:CDS | 45.0% | |
| AGACAAGGAAGAACCCTCTA+TGG | - | Chr3:67162707-67162726 | MsG0380015234.01.T01:CDS | 45.0% | |
| GAAGATCTTCAAGACACCTC+TGG | + | Chr3:67162597-67162616 | None:intergenic | 45.0% | |
| GGAAGAACCCTCTATGGATT+TGG | - | Chr3:67162713-67162732 | MsG0380015234.01.T01:CDS | 45.0% | |
| TGATACCCTGGATGTTATCG+CGG | + | Chr3:67162502-67162521 | None:intergenic | 45.0% | |
| AGTTCTCCGCGATAACATCC+AGG | - | Chr3:67162493-67162512 | MsG0380015234.01.T01:CDS | 50.0% | |
| CAAGCCTGCAATTCGTCGAT+TGG | - | Chr3:67162523-67162542 | MsG0380015234.01.T01:CDS | 50.0% | |
| CAATCGACGAATTGCAGGCT+TGG | + | Chr3:67162525-67162544 | None:intergenic | 50.0% | |
| CATACACTGAGCATGCTAGG+AGG | - | Chr3:67162645-67162664 | MsG0380015234.01.T01:CDS | 50.0% | |
| GGAGGTAAGGGACTTGGAAA+GGG | - | Chr3:67162449-67162468 | MsG0380015234.01.T01:CDS | 50.0% | |
| GTTCTCCGCGATAACATCCA+GGG | - | Chr3:67162494-67162513 | MsG0380015234.01.T01:CDS | 50.0% | |
| TGAGCATGCTAGGAGGAAGA+CGG | - | Chr3:67162652-67162671 | MsG0380015234.01.T01:CDS | 50.0% | |
| ! | ATTCGTCGATTGGCGAGAAG+AGG | - | Chr3:67162533-67162552 | MsG0380015234.01.T01:CDS | 50.0% |
| ! | GGCGGTGTTAAGAGAATCAG+TGG | - | Chr3:67162554-67162573 | MsG0380015234.01.T01:CDS | 50.0% |
| ! | TTGGAAAGGGAGGTGCAAAG+AGG | - | Chr3:67162462-67162481 | MsG0380015234.01.T01:CDS | 50.0% |
| AGGTCGTGGAAAGGGAGGTA+AGG | - | Chr3:67162436-67162455 | MsG0380015234.01.T01:CDS | 55.0% | |
| ATGTCAGGTCGTGGAAAGGG+AGG | - | Chr3:67162431-67162450 | MsG0380015234.01.T01:CDS | 55.0% | |
| CTCGCCAATCGACGAATTGC+AGG | + | Chr3:67162530-67162549 | None:intergenic | 55.0% | |
| GGAAAGGGAGGTAAGGGACT+TGG | - | Chr3:67162443-67162462 | MsG0380015234.01.T01:CDS | 55.0% | |
| GGGAGGTAAGGGACTTGGAA+AGG | - | Chr3:67162448-67162467 | MsG0380015234.01.T01:CDS | 55.0% | |
| GGTAAGGGACTTGGAAAGGG+AGG | - | Chr3:67162452-67162471 | MsG0380015234.01.T01:CDS | 55.0% | |
| GGTCGTGGAAAGGGAGGTAA+GGG | - | Chr3:67162437-67162456 | MsG0380015234.01.T01:CDS | 55.0% | |
| ! | AGGGAGGTGCAAAGAGGCAT+AGG | - | Chr3:67162468-67162487 | MsG0380015234.01.T01:CDS | 55.0% |
| !! | TTGCAGGCTTGGTGATACCC+TGG | + | Chr3:67162514-67162533 | None:intergenic | 55.0% |
| GAGGAAGACGGTGACAGCCA+TGG | - | Chr3:67162664-67162683 | MsG0380015234.01.T01:CDS | 60.0% | |
| ! | CGTCGATTGGCGAGAAGAGG+CGG | - | Chr3:67162536-67162555 | MsG0380015234.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 67162431 | 67162742 | 67162431 | ID=MsG0380015234.01;Name=MsG0380015234.01 |
| Chr3 | mRNA | 67162431 | 67162742 | 67162431 | ID=MsG0380015234.01.T01;Parent=MsG0380015234.01;Name=MsG0380015234.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr3 | exon | 67162431 | 67162742 | 67162431 | ID=MsG0380015234.01.T01:exon:8885;Parent=MsG0380015234.01.T01 |
| Chr3 | CDS | 67162431 | 67162742 | 67162431 | ID=MsG0380015234.01.T01:cds;Parent=MsG0380015234.01.T01 |
| Gene Sequence |
| Protein sequence |
