AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014681.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014681.01.T01 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0380014681.01.T01 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.74e-66 204
MsG0380014681.01.T01 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.23e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014681.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT5G59690 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT3G46320 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT3G45930 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT3G53730 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT2G28740 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT1G07660 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0380014681.01.T01 AT1G07660 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTGTCTTGAAGATCTTCT+TGG 0.405705 3:-58347364 MsG0380014681.01.T01:CDS
AGACAGGGAAGAACTCTCTA+TGG 0.431453 3:-58347255 MsG0380014681.01.T01:CDS
GGAAGAACTCTCTATGGTTT+CGG 0.433663 3:-58347249 MsG0380014681.01.T01:CDS
AAAATGTCAGGTCGTGGAAA+GGG 0.434071 3:-58347534 MsG0380014681.01.T01:CDS
CAATCGACGAATAGCAGGTT+TGG 0.439411 3:+58347440 None:intergenic
TGTCTATGCTTTGAAGAGAC+AGG 0.458633 3:-58347271 MsG0380014681.01.T01:CDS
GGTAAGGGTCTGGGAAAGGG+AGG 0.476615 3:-58347510 MsG0380014681.01.T01:CDS
CTTGCCAATCGACGAATAGC+AGG 0.487494 3:+58347435 None:intergenic
AGGGAGGTGCAAAGAGGCAT+AGG 0.498985 3:-58347494 MsG0380014681.01.T01:CDS
CTCCCGAAAATGTCAGGTCG+TGG 0.510898 3:-58347540 None:intergenic
GAAGATCTTCAAGACACCTC+TGG 0.510902 3:+58347368 None:intergenic
GGTTCTTCGTGATAACATCC+AGG 0.535931 3:-58347469 MsG0380014681.01.T01:CDS
AAATGTCAGGTCGTGGAAAG+GGG 0.537019 3:-58347533 MsG0380014681.01.T01:CDS
CAAACCTGCTATTCGTCGAT+TGG 0.553041 3:-58347439 MsG0380014681.01.T01:CDS
GAGGAAGACGGTGACAGCCA+TGG 0.561434 3:-58347298 MsG0380014681.01.T01:CDS
CGTCGATTGGCAAGAAGAGG+CGG 0.578876 3:-58347426 MsG0380014681.01.T01:CDS
GTCTATGCTTTGAAGAGACA+GGG 0.584038 3:-58347270 MsG0380014681.01.T01:CDS
AATGTCAGGTCGTGGAAAGG+GGG 0.584231 3:-58347532 MsG0380014681.01.T01:CDS
AGGTGCAAAGAGGCATAGGA+AGG 0.586086 3:-58347490 MsG0380014681.01.T01:CDS
TGACATACACTGAGCACGCA+AGG 0.587222 3:-58347320 MsG0380014681.01.T01:CDS
GAAAATGTCAGGTCGTGGAA+AGG 0.589881 3:-58347535 None:intergenic
AATCAGTGGATTGATCTATG+AGG 0.593356 3:-58347394 MsG0380014681.01.T01:CDS
TAGCAGGTTTGGTGATACCC+TGG 0.596796 3:+58347451 None:intergenic
ATGTCAGGTCGTGGAAAGGG+GGG 0.615792 3:-58347531 MsG0380014681.01.T01:CDS
ATTCGTCGATTGGCAAGAAG+AGG 0.622105 3:-58347429 MsG0380014681.01.T01:CDS
AGAACTCTCTATGGTTTCGG+AGG 0.626708 3:-58347246 MsG0380014681.01.T01:CDS
CAAAGCATAGACAACATCCA+TGG 0.629842 3:+58347281 None:intergenic
GGCGGTGTGAAAAGAATCAG+TGG 0.634187 3:-58347408 MsG0380014681.01.T01:CDS
CAGTGTATGTCACAGCATCA+CGG 0.646580 3:+58347331 None:intergenic
GTTCTTCGTGATAACATCCA+GGG 0.651580 3:-58347468 MsG0380014681.01.T01:CDS
TGAGCACGCAAGGAGGAAGA+CGG 0.652638 3:-58347310 MsG0380014681.01.T01:CDS
TGGGAAAGGGAGGTGCAAAG+AGG 0.663580 3:-58347500 MsG0380014681.01.T01:CDS
TTGATCTATGAGGAAACCAG+AGG 0.700822 3:-58347384 MsG0380014681.01.T01:CDS
CATACACTGAGCACGCAAGG+AGG 0.730442 3:-58347317 MsG0380014681.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAGTGGATTGATCTATG+AGG - Chr3:58347379-58347398 MsG0380014681.01.T01:CDS 35.0%
AGGTGTCTTGAAGATCTTCT+TGG - Chr3:58347409-58347428 MsG0380014681.01.T01:CDS 40.0%
CAAAGCATAGACAACATCCA+TGG + Chr3:58347495-58347514 None:intergenic 40.0%
GGAAGAACTCTCTATGGTTT+CGG - Chr3:58347524-58347543 MsG0380014681.01.T01:CDS 40.0%
GTTCTTCGTGATAACATCCA+GGG - Chr3:58347305-58347324 MsG0380014681.01.T01:CDS 40.0%
TTGATCTATGAGGAAACCAG+AGG - Chr3:58347389-58347408 MsG0380014681.01.T01:CDS 40.0%
!! GTCTATGCTTTGAAGAGACA+GGG - Chr3:58347503-58347522 MsG0380014681.01.T01:CDS 40.0%
!! TGTCTATGCTTTGAAGAGAC+AGG - Chr3:58347502-58347521 MsG0380014681.01.T01:CDS 40.0%
AGAACTCTCTATGGTTTCGG+AGG - Chr3:58347527-58347546 MsG0380014681.01.T01:CDS 45.0%
AGACAGGGAAGAACTCTCTA+TGG - Chr3:58347518-58347537 MsG0380014681.01.T01:CDS 45.0%
CAAACCTGCTATTCGTCGAT+TGG - Chr3:58347334-58347353 MsG0380014681.01.T01:CDS 45.0%
CAATCGACGAATAGCAGGTT+TGG + Chr3:58347336-58347355 None:intergenic 45.0%
CAGTGTATGTCACAGCATCA+CGG + Chr3:58347445-58347464 None:intergenic 45.0%
GAAGATCTTCAAGACACCTC+TGG + Chr3:58347408-58347427 None:intergenic 45.0%
GGTTCTTCGTGATAACATCC+AGG - Chr3:58347304-58347323 MsG0380014681.01.T01:CDS 45.0%
! ATTCGTCGATTGGCAAGAAG+AGG - Chr3:58347344-58347363 MsG0380014681.01.T01:CDS 45.0%
CTTGCCAATCGACGAATAGC+AGG + Chr3:58347341-58347360 None:intergenic 50.0%
GGCGGTGTGAAAAGAATCAG+TGG - Chr3:58347365-58347384 MsG0380014681.01.T01:CDS 50.0%
TGACATACACTGAGCACGCA+AGG - Chr3:58347453-58347472 MsG0380014681.01.T01:CDS 50.0%
! AGGTGCAAAGAGGCATAGGA+AGG - Chr3:58347283-58347302 MsG0380014681.01.T01:CDS 50.0%
!! TAGCAGGTTTGGTGATACCC+TGG + Chr3:58347325-58347344 None:intergenic 50.0%
ATGTCAGGTCGTGGAAAGGG+GGG - Chr3:58347242-58347261 MsG0380014681.01.T01:CDS 55.0%
CATACACTGAGCACGCAAGG+AGG - Chr3:58347456-58347475 MsG0380014681.01.T01:CDS 55.0%
TGAGCACGCAAGGAGGAAGA+CGG - Chr3:58347463-58347482 MsG0380014681.01.T01:CDS 55.0%
! AGGGAGGTGCAAAGAGGCAT+AGG - Chr3:58347279-58347298 MsG0380014681.01.T01:CDS 55.0%
! CGTCGATTGGCAAGAAGAGG+CGG - Chr3:58347347-58347366 MsG0380014681.01.T01:CDS 55.0%
! TGGGAAAGGGAGGTGCAAAG+AGG - Chr3:58347273-58347292 MsG0380014681.01.T01:CDS 55.0%
AGGTCGTGGAAAGGGGGGTA+AGG - Chr3:58347247-58347266 MsG0380014681.01.T01:CDS 60.0%
GAGGAAGACGGTGACAGCCA+TGG - Chr3:58347475-58347494 MsG0380014681.01.T01:CDS 60.0%
GGAAAGGGGGGTAAGGGTCT+GGG - Chr3:58347254-58347273 MsG0380014681.01.T01:CDS 60.0%
GGGGGTAAGGGTCTGGGAAA+GGG - Chr3:58347260-58347279 MsG0380014681.01.T01:CDS 60.0%
GGTAAGGGTCTGGGAAAGGG+AGG - Chr3:58347263-58347282 MsG0380014681.01.T01:CDS 60.0%
GGTCGTGGAAAGGGGGGTAA+GGG - Chr3:58347248-58347267 MsG0380014681.01.T01:CDS 60.0%
TGGAAAGGGGGGTAAGGGTC+TGG - Chr3:58347253-58347272 MsG0380014681.01.T01:CDS 60.0%
GGGGGGTAAGGGTCTGGGAA+AGG - Chr3:58347259-58347278 MsG0380014681.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 58347242 58347553 58347242 ID=MsG0380014681.01;Name=MsG0380014681.01
Chr3 mRNA 58347242 58347553 58347242 ID=MsG0380014681.01.T01;Parent=MsG0380014681.01;Name=MsG0380014681.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|103
Chr3 exon 58347242 58347553 58347242 ID=MsG0380014681.01.T01:exon:11310;Parent=MsG0380014681.01.T01
Chr3 CDS 58347242 58347553 58347242 ID=MsG0380014681.01.T01:cds;Parent=MsG0380014681.01.T01
Gene Sequence

>MsG0380014681.01.T01

ATGTCAGGTCGTGGAAAGGGGGGTAAGGGTCTGGGAAAGGGAGGTGCAAAGAGGCATAGGAAGGTTCTTCGTGATAACATCCAGGGTATCACCAAACCTGCTATTCGTCGATTGGCAAGAAGAGGCGGTGTGAAAAGAATCAGTGGATTGATCTATGAGGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAAAATGTCATCCGTGATGCTGTGACATACACTGAGCACGCAAGGAGGAAGACGGTGACAGCCATGGATGTTGTCTATGCTTTGAAGAGACAGGGAAGAACTCTCTATGGTTTCGGAGGTTGA

Protein sequence

>MsG0380014681.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*