AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020879.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020879.01.T01 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0480020879.01.T01 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.74e-66 204
MsG0480020879.01.T01 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.23e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020879.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT5G59690 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT3G46320 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT3G45930 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT3G53730 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT2G28740 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT1G07660 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0480020879.01.T01 AT1G07660 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 39 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAACCCTCTACGGATTT+GGG 0.267091 4:+50043817 MsG0480020879.01.T01:CDS
GGAAGAACCCTCTACGGATT+TGG 0.284984 4:+50043816 MsG0480020879.01.T01:CDS
TGATTTCTGATCAAGATGTC+TGG 0.330252 4:+50043519 None:intergenic
TGGCGTGTTGAAGATCTTCT+TGG 0.331421 4:+50043701 MsG0480020879.01.T01:CDS
CTTCTTGGAGAATGTGATTA+GGG 0.347463 4:+50043716 MsG0480020879.01.T01:CDS
GGAAAGGGTTTGGGAAAGGG+AGG 0.361300 4:+50043555 MsG0480020879.01.T01:CDS
TCTTCTTGGAGAATGTGATT+AGG 0.365922 4:+50043715 MsG0480020879.01.T01:CDS
GGAAAAGGAGGAAAGGGTTT+GGG 0.378357 4:+50043546 MsG0480020879.01.T01:CDS
TATCAGTGGTTTGATTTATG+AGG 0.387367 4:+50043671 MsG0480020879.01.T01:CDS
TGGAAAAGGAGGAAAGGGTT+TGG 0.387838 4:+50043545 MsG0480020879.01.T01:CDS
GGAGGAAAGGGTTTGGGAAA+GGG 0.411122 4:+50043552 MsG0480020879.01.T01:CDS
AAGATGTCTGGGCGTGGAAA+AGG 0.429435 4:+50043531 None:intergenic
AGGAGGAAAGGGTTTGGGAA+AGG 0.444877 4:+50043551 MsG0480020879.01.T01:CDS
CTTACACTGAGCACGCTAGA+AGG 0.481731 4:+50043748 MsG0480020879.01.T01:CDS
GACGGTTACCGCCATGGATG+TGG 0.492152 4:+50043773 MsG0480020879.01.T01:CDS
TCGCAGGCTTTGTGATTCCC+TGG 0.493877 4:-50043614 None:intergenic
GGTTTATGCTCTCAAGAGAC+AGG 0.499176 4:+50043794 MsG0480020879.01.T01:CDS
GGTGCTTCGTGACAACATCC+AGG 0.512203 4:+50043596 MsG0480020879.01.T01:CDS
GATTTCTGATCAAGATGTCT+GGG 0.513702 4:+50043520 None:intergenic
TGTTGTCACGAAGCACCTTA+CGG 0.540622 4:-50043590 None:intergenic
CGTCGTCTTGCTCGTAGAGG+AGG 0.557881 4:+50043639 MsG0480020879.01.T01:CDS
AAGAACCCTCTACGGATTTG+GGG 0.565022 4:+50043818 MsG0480020879.01.T01:CDS
AAGGAAGACGGTTACCGCCA+TGG 0.565591 4:+50043767 MsG0480020879.01.T01:CDS
CTGATCAAGATGTCTGGGCG+TGG 0.573727 4:+50043525 None:intergenic
AGGAGCAAAACGACACCGTA+AGG 0.575994 4:+50043575 MsG0480020879.01.T01:CDS
CGAGCAAGACGACGAATCGC+AGG 0.578319 4:-50043630 None:intergenic
GGGCGTGGAAAAGGAGGAAA+GGG 0.587932 4:+50043540 MsG0480020879.01.T01:CDS
GGAGGAGTGAAGCGTATCAG+TGG 0.591400 4:+50043657 MsG0480020879.01.T01:CDS
ATGTCTGGGCGTGGAAAAGG+AGG 0.593086 4:+50043534 MsG0480020879.01.T01:CDS
AGACAGGGAAGAACCCTCTA+CGG 0.600202 4:+50043810 MsG0480020879.01.T01:CDS
TTGATTTATGAGGAGACTCG+TGG 0.604489 4:+50043681 MsG0480020879.01.T01:CDS
TGGGCGTGGAAAAGGAGGAA+AGG 0.624585 4:+50043539 MsG0480020879.01.T01:CDS
GAGAGCATAAACCACATCCA+TGG 0.625099 4:-50043784 None:intergenic
AGAACCCTCTACGGATTTGG+GGG 0.641482 4:+50043819 MsG0480020879.01.T01:CDS
GTGCTTCGTGACAACATCCA+GGG 0.652644 4:+50043597 MsG0480020879.01.T01:CDS
ATTCGTCGTCTTGCTCGTAG+AGG 0.654201 4:+50043636 MsG0480020879.01.T01:CDS
TGAGCACGCTAGAAGGAAGA+CGG 0.664565 4:+50043755 MsG0480020879.01.T01:CDS
GTTTATGCTCTCAAGAGACA+GGG 0.674567 4:+50043795 MsG0480020879.01.T01:CDS
AGCATAAACCACATCCATGG+CGG 0.701499 4:-50043781 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATCAGTGGTTTGATTTATG+AGG + Chr4:50043671-50043690 MsG0480020879.01.T01:CDS 30.0%
CTTCTTGGAGAATGTGATTA+GGG + Chr4:50043716-50043735 MsG0480020879.01.T01:CDS 35.0%
TCTTCTTGGAGAATGTGATT+AGG + Chr4:50043715-50043734 MsG0480020879.01.T01:CDS 35.0%
GTTTATGCTCTCAAGAGACA+GGG + Chr4:50043795-50043814 MsG0480020879.01.T01:CDS 40.0%
TTGATTTATGAGGAGACTCG+TGG + Chr4:50043681-50043700 MsG0480020879.01.T01:CDS 40.0%
AAGAACCCTCTACGGATTTG+GGG + Chr4:50043818-50043837 MsG0480020879.01.T01:CDS 45.0%
AGCATAAACCACATCCATGG+CGG - Chr4:50043784-50043803 None:intergenic 45.0%
GAAGAACCCTCTACGGATTT+GGG + Chr4:50043817-50043836 MsG0480020879.01.T01:CDS 45.0%
GGAAAAGGAGGAAAGGGTTT+GGG + Chr4:50043546-50043565 MsG0480020879.01.T01:CDS 45.0%
GGTTTATGCTCTCAAGAGAC+AGG + Chr4:50043794-50043813 MsG0480020879.01.T01:CDS 45.0%
TGGAAAAGGAGGAAAGGGTT+TGG + Chr4:50043545-50043564 MsG0480020879.01.T01:CDS 45.0%
TGGCGTGTTGAAGATCTTCT+TGG + Chr4:50043701-50043720 MsG0480020879.01.T01:CDS 45.0%
! GAGAGCATAAACCACATCCA+TGG - Chr4:50043787-50043806 None:intergenic 45.0%
! TGTTGTCACGAAGCACCTTA+CGG - Chr4:50043593-50043612 None:intergenic 45.0%
AGAACCCTCTACGGATTTGG+GGG + Chr4:50043819-50043838 MsG0480020879.01.T01:CDS 50.0%
AGACAGGGAAGAACCCTCTA+CGG + Chr4:50043810-50043829 MsG0480020879.01.T01:CDS 50.0%
AGGAGCAAAACGACACCGTA+AGG + Chr4:50043575-50043594 MsG0480020879.01.T01:CDS 50.0%
AGGAGGAAAGGGTTTGGGAA+AGG + Chr4:50043551-50043570 MsG0480020879.01.T01:CDS 50.0%
ATTCGTCGTCTTGCTCGTAG+AGG + Chr4:50043636-50043655 MsG0480020879.01.T01:CDS 50.0%
CTAACCCCCAAATCCGTAGA+GGG - Chr4:50043826-50043845 None:intergenic 50.0%
CTTACACTGAGCACGCTAGA+AGG + Chr4:50043748-50043767 MsG0480020879.01.T01:CDS 50.0%
GGAAGAACCCTCTACGGATT+TGG + Chr4:50043816-50043835 MsG0480020879.01.T01:CDS 50.0%
GGAGGAAAGGGTTTGGGAAA+GGG + Chr4:50043552-50043571 MsG0480020879.01.T01:CDS 50.0%
GTGCTTCGTGACAACATCCA+GGG + Chr4:50043597-50043616 MsG0480020879.01.T01:CDS 50.0%
TGAGCACGCTAGAAGGAAGA+CGG + Chr4:50043755-50043774 MsG0480020879.01.T01:CDS 50.0%
AAGGAAGACGGTTACCGCCA+TGG + Chr4:50043767-50043786 MsG0480020879.01.T01:CDS 55.0%
ATGTCTGGGCGTGGAAAAGG+AGG + Chr4:50043534-50043553 MsG0480020879.01.T01:CDS 55.0%
GGAAAGGGTTTGGGAAAGGG+AGG + Chr4:50043555-50043574 MsG0480020879.01.T01:CDS 55.0%
GGAGGAGTGAAGCGTATCAG+TGG + Chr4:50043657-50043676 MsG0480020879.01.T01:CDS 55.0%
GGGCGTGGAAAAGGAGGAAA+GGG + Chr4:50043540-50043559 MsG0480020879.01.T01:CDS 55.0%
GGTGCTTCGTGACAACATCC+AGG + Chr4:50043596-50043615 MsG0480020879.01.T01:CDS 55.0%
TGGGCGTGGAAAAGGAGGAA+AGG + Chr4:50043539-50043558 MsG0480020879.01.T01:CDS 55.0%
! TCGCAGGCTTTGTGATTCCC+TGG - Chr4:50043617-50043636 None:intergenic 55.0%
CGAGCAAGACGACGAATCGC+AGG - Chr4:50043633-50043652 None:intergenic 60.0%
CGTCGTCTTGCTCGTAGAGG+AGG + Chr4:50043639-50043658 MsG0480020879.01.T01:CDS 60.0%
GACGGTTACCGCCATGGATG+TGG + Chr4:50043773-50043792 MsG0480020879.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 50043534 50043845 50043534 ID=MsG0480020879.01;Name=MsG0480020879.01
Chr4 mRNA 50043534 50043845 50043534 ID=MsG0480020879.01.T01;Parent=MsG0480020879.01;Name=MsG0480020879.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|103
Chr4 exon 50043534 50043845 50043534 ID=MsG0480020879.01.T01:exon:836;Parent=MsG0480020879.01.T01
Chr4 CDS 50043534 50043845 50043534 ID=MsG0480020879.01.T01:cds;Parent=MsG0480020879.01.T01
Gene Sequence

>MsG0480020879.01.T01

ATGTCTGGGCGTGGAAAAGGAGGAAAGGGTTTGGGAAAGGGAGGAGCAAAACGACACCGTAAGGTGCTTCGTGACAACATCCAGGGAATCACAAAGCCTGCGATTCGTCGTCTTGCTCGTAGAGGAGGAGTGAAGCGTATCAGTGGTTTGATTTATGAGGAGACTCGTGGCGTGTTGAAGATCTTCTTGGAGAATGTGATTAGGGATGCTGTGACTTACACTGAGCACGCTAGAAGGAAGACGGTTACCGCCATGGATGTGGTTTATGCTCTCAAGAGACAGGGAAGAACCCTCTACGGATTTGGGGGTTAG

Protein sequence

>MsG0480020879.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*