AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016102.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016102.01.T01 MTR_3g083470 84.733 131 14 2 1 127 33 161 6.74e-53 167
MsG0380016102.01.T01 MTR_8g105230 48.101 79 31 1 59 127 36 114 4.39e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTACGGAGAAACAAAAT+TGG 0.349996 3:-79146986 MsG0380016102.01.T01:CDS
CAATGTCAACACATTGGAAT+TGG 0.350732 3:-79146957 MsG0380016102.01.T01:CDS
AGAGGAAATGGAAAACTTAA+AGG 0.354653 3:-79146759 MsG0380016102.01.T01:CDS
CTATGAATCTGAAGAGGAAA+TGG 0.373890 3:-79146771 MsG0380016102.01.T01:CDS
AAAGATGACAACAACAATAA+TGG 0.375899 3:+79147015 None:intergenic
ATAGATGGTGTATTGTTGTT+TGG 0.388629 3:+79147048 None:intergenic
TCAGATCTGAGATCGATAAC+TGG 0.401362 3:-79146878 MsG0380016102.01.T01:CDS
ATAACTGGAAAGTTGGATAT+AGG 0.436939 3:-79146863 MsG0380016102.01.T01:CDS
GATGACGTTGATGGCATTGA+TGG 0.458924 3:-79146839 MsG0380016102.01.T01:CDS
TGCTAACAAAACAGCTAAAA+CGG 0.468639 3:+79147102 None:intergenic
GAGATCGATAACTGGAAAGT+TGG 0.474110 3:-79146870 MsG0380016102.01.T01:CDS
TCATCTTTCTTAATCTGCTA+CGG 0.479201 3:-79147000 MsG0380016102.01.T01:CDS
AATGGAGGAGATGAAATAGA+TGG 0.486385 3:+79147033 None:intergenic
GATATAGGAGATGACGTTGA+TGG 0.498114 3:-79146848 MsG0380016102.01.T01:CDS
GCACTGAATTACACAGAAGC+TGG 0.501469 3:-79147082 MsG0380016102.01.T01:CDS
GTGTATGACATTGATGGTGA+TGG 0.505283 3:-79146726 MsG0380016102.01.T01:CDS
CAACACATTGGAATTGGAGA+TGG 0.533055 3:-79146951 MsG0380016102.01.T01:CDS
AACAAACTATGAATCTGAAG+AGG 0.586456 3:-79146777 MsG0380016102.01.T01:CDS
CAATGTCAATGTCAACACAT+TGG 0.601927 3:-79146963 MsG0380016102.01.T01:CDS
TTCAAGAAATTGGACACAGA+TGG 0.611437 3:-79146920 MsG0380016102.01.T01:CDS
GATGACAACAACAATAATGG+AGG 0.629703 3:+79147018 None:intergenic
AAATTGGACACAGATGGTGA+TGG 0.649231 3:-79146914 MsG0380016102.01.T01:CDS
GACGTTGATGGCATTGATGG+AGG 0.660924 3:-79146836 MsG0380016102.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGATGACAACAACAATAA+TGG + Chr3:79146788-79146807 None:intergenic 25.0%
AACAAACTATGAATCTGAAG+AGG - Chr3:79147023-79147042 MsG0380016102.01.T01:CDS 30.0%
AGAGGAAATGGAAAACTTAA+AGG - Chr3:79147041-79147060 MsG0380016102.01.T01:CDS 30.0%
ATAACTGGAAAGTTGGATAT+AGG - Chr3:79146937-79146956 MsG0380016102.01.T01:CDS 30.0%
GGAAAAACTCTTCAAGAAAT+TGG - Chr3:79146870-79146889 MsG0380016102.01.T01:CDS 30.0%
TCATCTTTCTTAATCTGCTA+CGG - Chr3:79146800-79146819 MsG0380016102.01.T01:CDS 30.0%
TGCTAACAAAACAGCTAAAA+CGG + Chr3:79146701-79146720 None:intergenic 30.0%
! ATAGATGGTGTATTGTTGTT+TGG + Chr3:79146755-79146774 None:intergenic 30.0%
! TTTTCTGTGTATGACATTGA+TGG - Chr3:79147068-79147087 MsG0380016102.01.T01:CDS 30.0%
AATGGAGGAGATGAAATAGA+TGG + Chr3:79146770-79146789 None:intergenic 35.0%
CAATGTCAACACATTGGAAT+TGG - Chr3:79146843-79146862 MsG0380016102.01.T01:CDS 35.0%
CAATGTCAATGTCAACACAT+TGG - Chr3:79146837-79146856 MsG0380016102.01.T01:CDS 35.0%
CTATGAATCTGAAGAGGAAA+TGG - Chr3:79147029-79147048 MsG0380016102.01.T01:CDS 35.0%
GATGACAACAACAATAATGG+AGG + Chr3:79146785-79146804 None:intergenic 35.0%
TTCAAGAAATTGGACACAGA+TGG - Chr3:79146880-79146899 MsG0380016102.01.T01:CDS 35.0%
! AAGCGTATTAAGCTCTTTTG+CGG + Chr3:79147108-79147127 None:intergenic 35.0%
AAATTGGACACAGATGGTGA+TGG - Chr3:79146886-79146905 MsG0380016102.01.T01:CDS 40.0%
CAACACATTGGAATTGGAGA+TGG - Chr3:79146849-79146868 MsG0380016102.01.T01:CDS 40.0%
CTGCTACGGAGAAACAAAAT+TGG - Chr3:79146814-79146833 MsG0380016102.01.T01:CDS 40.0%
GAGATCGATAACTGGAAAGT+TGG - Chr3:79146930-79146949 MsG0380016102.01.T01:CDS 40.0%
GATATAGGAGATGACGTTGA+TGG - Chr3:79146952-79146971 MsG0380016102.01.T01:CDS 40.0%
! TCAGATCTGAGATCGATAAC+TGG - Chr3:79146922-79146941 MsG0380016102.01.T01:CDS 40.0%
!! GTGTATGACATTGATGGTGA+TGG - Chr3:79147074-79147093 MsG0380016102.01.T01:CDS 40.0%
GCACTGAATTACACAGAAGC+TGG - Chr3:79146718-79146737 MsG0380016102.01.T01:CDS 45.0%
!! GATGACGTTGATGGCATTGA+TGG - Chr3:79146961-79146980 MsG0380016102.01.T01:CDS 45.0%
!! GACGTTGATGGCATTGATGG+AGG - Chr3:79146964-79146983 MsG0380016102.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 79146691 79147131 79146691 ID=MsG0380016102.01;Name=MsG0380016102.01
Chr3 mRNA 79146691 79147131 79146691 ID=MsG0380016102.01.T01;Parent=MsG0380016102.01;Name=MsG0380016102.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|146
Chr3 exon 79146691 79147131 79146691 ID=MsG0380016102.01.T01:exon:17901;Parent=MsG0380016102.01.T01
Chr3 CDS 79146691 79147131 79146691 ID=MsG0380016102.01.T01:cds;Parent=MsG0380016102.01.T01
Gene Sequence

>MsG0380016102.01.T01

ATGATTGCCGTTTTAGCTGTTTTGTTAGCACTGAATTACACAGAAGCTGGAAACAAATTTTCCAAACAACAATACACCATCTATTTCATCTCCTCCATTATTGTTGTTGTCATCTTTCTTAATCTGCTACGGAGAAACAAAATTGGCAATGTCAATGTCAACACATTGGAATTGGAGATGGAAAAACTCTTCAAGAAATTGGACACAGATGGTGATGGAAAGATATCGATCTCAGATCTGAGATCGATAACTGGAAAGTTGGATATAGGAGATGACGTTGATGGCATTGATGGAGGATCTATCACGCTTCAAGAGTTTATTGAGCTAAGCACAACAAACTATGAATCTGAAGAGGAAATGGAAAACTTAAAGGCACGTTTTCTGTGTATGACATTGATGGTGATGGTTTCATTACCGCAAAAGAGCTTAATACGCTTATGA

Protein sequence

>MsG0380016102.01.T01

MIAVLAVLLALNYTEAGNKFSKQQYTIYFISSIIVVVIFLNLLRRNKIGNVNVNTLELEMEKLFKKLDTDGDGKISISDLRSITGKLDIGDDVDGIDGGSITLQEFIELSTTNYESEEEMENLKARFLCMTLMVMVSLPQKSLIRL*