Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030856.01.T01 | XP_003618565.2 | 87.5 | 56 | 7 | 0 | 16 | 71 | 532 | 587 | 3.47E-16 | 82.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030856.01.T01 | G7KLC0 | 87.500 | 56 | 7 | 0 | 16 | 71 | 532 | 587 | 1.66e-16 | 82.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001892.01 | MsG0680030856.01 | 0.825951 | 3.542413e-54 | 3.276606e-51 |
| MsG0180004605.01 | MsG0680030856.01 | -0.800893 | 1.163742e-48 | 5.481938e-46 |
| MsG0180004743.01 | MsG0680030856.01 | 0.809816 | 1.569464e-50 | 9.307723e-48 |
| MsG0180004765.01 | MsG0680030856.01 | 0.823260 | 1.524637e-53 | 1.306140e-50 |
| MsG0180005153.01 | MsG0680030856.01 | 0.815945 | 7.128856e-52 | 4.983478e-49 |
| MsG0180005575.01 | MsG0680030856.01 | 0.807597 | 4.676641e-50 | 2.615452e-47 |
| MsG0280010722.01 | MsG0680030856.01 | 0.804523 | 2.073347e-49 | 1.071325e-46 |
| MsG0380012067.01 | MsG0680030856.01 | 0.808456 | 3.069848e-50 | 1.756159e-47 |
| MsG0380014225.01 | MsG0680030856.01 | -0.806157 | 9.426581e-50 | 5.080144e-47 |
| MsG0680030856.01 | MsG0680034739.01 | 0.807268 | 5.491955e-50 | 3.045515e-47 |
| MsG0680030856.01 | MsG0680035498.01 | 0.808073 | 3.704067e-50 | 2.097633e-47 |
| MsG0680030856.01 | MsG0780037093.01 | 0.810818 | 9.542198e-51 | 5.810581e-48 |
| MsG0680030856.01 | MsG0780041259.01 | 0.821352 | 4.225709e-53 | 3.429633e-50 |
| MsG0680030856.01 | MsG0780041440.01 | 0.803555 | 3.296138e-49 | 1.661500e-46 |
| MsG0680030856.01 | MsG0880043480.01 | 0.811469 | 6.892543e-51 | 4.270101e-48 |
| MsG0680030856.01 | MsG0880045362.01 | 0.807733 | 4.375226e-50 | 2.455762e-47 |
| MsG0680030856.01 | MsG0880046333.01 | -0.803874 | 2.830523e-49 | 1.438507e-46 |
| MsG0480020780.01 | MsG0680030856.01 | 0.805724 | 1.162555e-49 | 6.196387e-47 |
| MsG0480021056.01 | MsG0680030856.01 | 0.820964 | 5.193198e-53 | 4.169988e-50 |
| MsG0480021195.01 | MsG0680030856.01 | 0.810709 | 1.007139e-50 | 6.115247e-48 |
| MsG0480021280.01 | MsG0680030856.01 | 0.809344 | 1.981894e-50 | 1.160763e-47 |
| MsG0580025987.01 | MsG0680030856.01 | 0.814358 | 1.604360e-51 | 1.074163e-48 |
| MsG0580026718.01 | MsG0680030856.01 | 0.805105 | 1.567588e-49 | 8.222689e-47 |
| MsG0580027925.01 | MsG0680030856.01 | 0.811626 | 6.373629e-51 | 3.964747e-48 |
| MsG0580029236.01 | MsG0680030856.01 | 0.810677 | 1.023479e-50 | 6.208996e-48 |
| MsG0580029924.01 | MsG0680030856.01 | 0.810664 | 1.030045e-50 | 6.246754e-48 |
| MsG0580029925.01 | MsG0680030856.01 | 0.823724 | 1.187539e-53 | 1.030955e-50 |
| MsG0280007955.01 | MsG0680030856.01 | 0.832750 | 7.910322e-56 | 8.924042e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030856.01.T01 | MTR_6g013050 | 87.500 | 56 | 7 | 0 | 16 | 71 | 532 | 587 | 4.20e-20 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTAAATCAGATTAGATTTA+AGG | 0.099349 | 6:-10569995 | MsG0680030856.01.T01:CDS |
| CTTGGGGTTGTCTTAATTTA+TGG | 0.160500 | 6:-10570030 | MsG0680030856.01.T01:CDS |
| TTAATTTCTCTATACAAATT+TGG | 0.181283 | 6:+10569823 | None:intergenic |
| TACAAACAAACTCTTTAATA+AGG | 0.239394 | 6:+10569945 | None:intergenic |
| CCTTTGTTGTATACAGTTAC+AGG | 0.244141 | 6:-10570228 | MsG0680030856.01.T01:CDS |
| TCATATCACAGTTAATCCTT+GGG | 0.399013 | 6:-10570047 | MsG0680030856.01.T01:CDS |
| ATCATATCACAGTTAATCCT+TGG | 0.404180 | 6:-10570048 | MsG0680030856.01.T01:CDS |
| ACAAACAAACTCTTTAATAA+GGG | 0.459427 | 6:+10569946 | None:intergenic |
| GTACTTGTCATCCTTTGTTG+AGG | 0.523019 | 6:-10569925 | MsG0680030856.01.T01:three_prime_UTR |
| GAGGCATTAAATTGTATACA+TGG | 0.524468 | 6:-10569906 | MsG0680030856.01.T01:three_prime_UTR |
| TGACTAGAAGCAGCAGAGTG+TGG | 0.582076 | 6:+10570188 | None:intergenic |
| ACAATTTAATGCCTCAACAA+AGG | 0.604029 | 6:+10569914 | None:intergenic |
| ACTGTATACAACAAAGGCAA+AGG | 0.615787 | 6:+10570234 | None:intergenic |
| CATATCACAGTTAATCCTTG+GGG | 0.619406 | 6:-10570046 | MsG0680030856.01.T01:CDS |
| CCTGTAACTGTATACAACAA+AGG | 0.637113 | 6:+10570228 | None:intergenic |
| CAATGAAGCTTACTTGAGAG+AGG | 0.642370 | 6:+10570165 | None:intergenic |
| CATAAATTAAGACAACCCCA+AGG | 0.719747 | 6:+10570031 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTTCTCTATACAAATT+TGG | + | Chr6:10570211-10570230 | None:intergenic | 15.0% |
| !! | TTCATCATGATTAAAATTAA+TGG | - | Chr6:10569919-10569938 | MsG0680030856.01.T01:three_prime_UTR | 15.0% |
| !!! | ACACATTTTTTGAAAATTAA+AGG | + | Chr6:10570180-10570199 | None:intergenic | 15.0% |
| !!! | TTTAATCATGATGAAAATTT+TGG | + | Chr6:10569914-10569933 | None:intergenic | 15.0% |
| !!! | TTTAATTTTCATTTTCTGTT+GGG | - | Chr6:10570151-10570170 | MsG0680030856.01.T01:intron | 15.0% |
| !! | ACAAACAAACTCTTTAATAA+GGG | + | Chr6:10570088-10570107 | None:intergenic | 20.0% |
| !! | TACAAACAAACTCTTTAATA+AGG | + | Chr6:10570089-10570108 | None:intergenic | 20.0% |
| !! | TGTAAATCAGATTAGATTTA+AGG | - | Chr6:10570036-10570055 | MsG0680030856.01.T01:CDS | 20.0% |
| !!! | CTTTAATTTTCATTTTCTGT+TGG | - | Chr6:10570150-10570169 | MsG0680030856.01.T01:intron | 20.0% |
| !!! | TTAATTTTCATTTTCTGTTG+GGG | - | Chr6:10570152-10570171 | MsG0680030856.01.T01:intron | 20.0% |
| ACAATTTAATGCCTCAACAA+AGG | + | Chr6:10570120-10570139 | None:intergenic | 30.0% | |
| ATCATATCACAGTTAATCCT+TGG | - | Chr6:10569983-10570002 | MsG0680030856.01.T01:exon | 30.0% | |
| TCATATCACAGTTAATCCTT+GGG | - | Chr6:10569984-10570003 | MsG0680030856.01.T01:exon | 30.0% | |
| ! | GAGGCATTAAATTGTATACA+TGG | - | Chr6:10570125-10570144 | MsG0680030856.01.T01:intron | 30.0% |
| ACTGTATACAACAAAGGCAA+AGG | + | Chr6:10569800-10569819 | None:intergenic | 35.0% | |
| CATAAATTAAGACAACCCCA+AGG | + | Chr6:10570003-10570022 | None:intergenic | 35.0% | |
| CATATCACAGTTAATCCTTG+GGG | - | Chr6:10569985-10570004 | MsG0680030856.01.T01:exon | 35.0% | |
| CCTGTAACTGTATACAACAA+AGG | + | Chr6:10569806-10569825 | None:intergenic | 35.0% | |
| CTTGGGGTTGTCTTAATTTA+TGG | - | Chr6:10570001-10570020 | MsG0680030856.01.T01:CDS | 35.0% | |
| TCAATTGAAGTTGTTCACAC+AGG | - | Chr6:10569949-10569968 | MsG0680030856.01.T01:three_prime_UTR | 35.0% | |
| ! | CCTTTGTTGTATACAGTTAC+AGG | - | Chr6:10569803-10569822 | MsG0680030856.01.T01:three_prime_UTR | 35.0% |
| !!! | ATTTTGGCTTTAGAGTGAAG+AGG | + | Chr6:10569898-10569917 | None:intergenic | 35.0% |
| CAATGAAGCTTACTTGAGAG+AGG | + | Chr6:10569869-10569888 | None:intergenic | 40.0% | |
| GTACTTGTCATCCTTTGTTG+AGG | - | Chr6:10570106-10570125 | MsG0680030856.01.T01:intron | 40.0% | |
| TGACTAGAAGCAGCAGAGTG+TGG | + | Chr6:10569846-10569865 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 10569753 | 10570300 | 10569753 | ID=MsG0680030856.01;Name=MsG0680030856.01 |
| Chr6 | mRNA | 10569753 | 10570300 | 10569753 | ID=MsG0680030856.01.T01;Parent=MsG0680030856.01;Name=MsG0680030856.01.T01;_AED=0.48;_eAED=0.53;_QI=0|1|0.5|1|0|0|2|237|71 |
| Chr6 | exon | 10569753 | 10570082 | 10569753 | ID=MsG0680030856.01.T01:exon:8073;Parent=MsG0680030856.01.T01 |
| Chr6 | exon | 10570178 | 10570300 | 10570178 | ID=MsG0680030856.01.T01:exon:8072;Parent=MsG0680030856.01.T01 |
| Chr6 | CDS | 10570178 | 10570300 | 10570178 | ID=MsG0680030856.01.T01:cds;Parent=MsG0680030856.01.T01 |
| Chr6 | CDS | 10569990 | 10570082 | 10569990 | ID=MsG0680030856.01.T01:cds;Parent=MsG0680030856.01.T01 |
| Chr6 | three_prime_UTR | 10569753 | 10569989 | 10569753 | ID=MsG0680030856.01.T01:three_prime_utr;Parent=MsG0680030856.01.T01 |
| Gene Sequence |
| Protein sequence |
