AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034739.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034739.01.T01 MTR_4g103250 37.805 82 51 0 41 122 59 140 7.52e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034739.01.T01 AT2G01940 52.991 117 43 4 40 146 240 354 9.11e-30 111
MsG0680034739.01.T01 AT2G01940 52.991 117 43 4 40 146 329 443 1.95e-29 111
MsG0680034739.01.T01 AT2G01940 52.991 117 43 4 40 146 330 444 2.03e-29 111
MsG0680034739.01.T01 AT1G25250 57.303 89 31 2 59 141 217 304 2.69e-26 101
MsG0680034739.01.T01 AT1G25250 57.303 89 31 2 59 141 272 359 6.96e-26 100
MsG0680034739.01.T01 AT1G25250 57.303 89 31 2 59 141 295 382 9.93e-26 100
MsG0680034739.01.T01 AT1G68130 49.485 97 33 2 60 141 229 324 3.31e-21 88.2
MsG0680034739.01.T01 AT1G68130 49.485 97 33 2 60 141 315 410 6.62e-21 88.2
MsG0680034739.01.T01 AT3G48550 40.244 82 49 0 41 122 76 157 8.06e-18 75.9

Find 18 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGAACTTGATAAAGCTTA+TGG 0.272526 6:+93760071 MsG0680034739.01.T01:CDS
AACAAGCAAAGGAACAATTA+AGG 0.285904 6:+93759934 MsG0680034739.01.T01:CDS
TCAACATGAAGATAACTCTT+TGG 0.326169 6:+93760169 MsG0680034739.01.T01:CDS
TTGCTATTGCTACTCTCCTT+TGG 0.344778 6:-93759858 None:intergenic
TTCACCACCTTCAGTAGTTA+TGG 0.411636 6:-93760213 None:intergenic
AGCAAAGAAACAAATTGAAA+TGG 0.432424 6:+93760001 MsG0680034739.01.T01:CDS
GTAGAAAATGTTGATGGAAA+AGG 0.444254 6:+93760236 MsG0680034739.01.T01:CDS
ACAAGCAAAGGAACAATTAA+GGG 0.459888 6:+93759935 MsG0680034739.01.T01:CDS
AAACGACATGGCTTTGTTGA+GGG 0.472598 6:+93759905 MsG0680034739.01.T01:CDS
GGAACAATTAAGGGTAGCTA+TGG 0.508901 6:+93759944 MsG0680034739.01.T01:CDS
GGTGAAGTAGAAAATGTTGA+TGG 0.512722 6:+93760230 MsG0680034739.01.T01:CDS
CAAACGACATGGCTTTGTTG+AGG 0.525521 6:+93759904 MsG0680034739.01.T01:CDS
TCTTCTGCCATAACTACTGA+AGG 0.549896 6:+93760206 MsG0680034739.01.T01:CDS
AAGCTTATGCTGAAGAAGCA+AGG 0.595645 6:+93759973 MsG0680034739.01.T01:CDS
GAGGGTTCAAGAACAAGCAA+AGG 0.630915 6:+93759923 MsG0680034739.01.T01:CDS
AAACTCAAACTCATCACCAA+AGG 0.637316 6:+93759842 MsG0680034739.01.T01:CDS
TCTGCCATAACTACTGAAGG+TGG 0.661155 6:+93760209 MsG0680034739.01.T01:CDS
ATCAACAACAACAAACGACA+TGG 0.665274 6:+93759893 MsG0680034739.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGCAAAGAAACAAATTGAAA+TGG + Chr6:93760001-93760020 MsG0680034739.01.T01:CDS 25.0%
AACAAGCAAAGGAACAATTA+AGG + Chr6:93759934-93759953 MsG0680034739.01.T01:CDS 30.0%
ACAAGCAAAGGAACAATTAA+GGG + Chr6:93759935-93759954 MsG0680034739.01.T01:CDS 30.0%
AGTGAACTTGATAAAGCTTA+TGG + Chr6:93760071-93760090 MsG0680034739.01.T01:CDS 30.0%
TCAACATGAAGATAACTCTT+TGG + Chr6:93760169-93760188 MsG0680034739.01.T01:CDS 30.0%
! GTAGAAAATGTTGATGGAAA+AGG + Chr6:93760236-93760255 MsG0680034739.01.T01:CDS 30.0%
AAACTCAAACTCATCACCAA+AGG + Chr6:93759842-93759861 MsG0680034739.01.T01:CDS 35.0%
ATCAACAACAACAAACGACA+TGG + Chr6:93759893-93759912 MsG0680034739.01.T01:CDS 35.0%
GGTGAAGTAGAAAATGTTGA+TGG + Chr6:93760230-93760249 MsG0680034739.01.T01:CDS 35.0%
AAGCTTATGCTGAAGAAGCA+AGG + Chr6:93759973-93759992 MsG0680034739.01.T01:CDS 40.0%
GGAACAATTAAGGGTAGCTA+TGG + Chr6:93759944-93759963 MsG0680034739.01.T01:CDS 40.0%
TCTTCTGCCATAACTACTGA+AGG + Chr6:93760206-93760225 MsG0680034739.01.T01:CDS 40.0%
TTCACCACCTTCAGTAGTTA+TGG - Chr6:93760216-93760235 None:intergenic 40.0%
! TTGCTATTGCTACTCTCCTT+TGG - Chr6:93759861-93759880 None:intergenic 40.0%
!! AAACGACATGGCTTTGTTGA+GGG + Chr6:93759905-93759924 MsG0680034739.01.T01:CDS 40.0%
GAGGGTTCAAGAACAAGCAA+AGG + Chr6:93759923-93759942 MsG0680034739.01.T01:CDS 45.0%
TCTGCCATAACTACTGAAGG+TGG + Chr6:93760209-93760228 MsG0680034739.01.T01:CDS 45.0%
!! CAAACGACATGGCTTTGTTG+AGG + Chr6:93759904-93759923 MsG0680034739.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr6 gene 93759807 93760274 93759807 ID=MsG0680034739.01;Name=MsG0680034739.01
Chr6 mRNA 93759807 93760274 93759807 ID=MsG0680034739.01.T01;Parent=MsG0680034739.01;Name=MsG0680034739.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|155
Chr6 exon 93759807 93760274 93759807 ID=MsG0680034739.01.T01:exon:8301;Parent=MsG0680034739.01.T01
Chr6 CDS 93759807 93760274 93759807 ID=MsG0680034739.01.T01:cds;Parent=MsG0680034739.01.T01
Gene Sequence

>MsG0680034739.01.T01

ATGAGTCATGAGAAAAATGAATCAAACAATATTAGAAACTCAAACTCATCACCAAAGGAGAGTAGCAATAGCAATGAGAAAGTTCAATCAACAACAACAAACGACATGGCTTTGTTGAGGGTTCAAGAACAAGCAAAGGAACAATTAAGGGTAGCTATGGCTGAAAAAGCTTATGCTGAAGAAGCAAGGAAACAAGCAAAGAAACAAATTGAAATGGCTGAACAAGAGTTCAACAATGCAAAGAGAATTAGACAACAAGCACAAAGTGAACTTGATAAAGCTTATGGATTGAAACAACATGCAATAAAGCAAATCAATTCAACTATGCTTCAAATCACTTGTCAAGCTTGCAAACAACAATTTCAACATGAAGATAACTCTTTGGTGTTGAGCTATGTTTCTTCTGCCATAACTACTGAAGGTGGTGAAGTAGAAAATGTTGATGGAAAAGGCAAAACAACTAATTAG

Protein sequence

>MsG0680034739.01.T01

MSHEKNESNNIRNSNSSPKESSNSNEKVQSTTTNDMALLRVQEQAKEQLRVAMAEKAYAEEARKQAKKQIEMAEQEFNNAKRIRQQAQSELDKAYGLKQHAIKQINSTMLQITCQACKQQFQHEDNSLVLSYVSSAITTEGGEVENVDGKGKTTN*