AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035498.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035498.01.T01 MTR_6g022830 96.403 139 5 0 1 139 534 672 7.48e-90 274
MsG0680035498.01.T01 MTR_6g023070 95.683 139 6 0 1 139 471 609 1.92e-80 248
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035498.01.T01 AT3G01750 53.488 129 46 5 9 128 535 658 4.06e-19 82.8
MsG0680035498.01.T01 AT3G01750 53.488 129 46 5 9 128 535 658 4.06e-19 82.8

Find 17 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTTCTCTTAACGAATTT+CGG 0.284326 6:+106860318 None:intergenic
ACAAAAGAACACTTTCTGTT+AGG 0.359130 6:-106860536 MsG0680035498.01.T01:CDS
GCAAATCTCTTCTTAGCATT+AGG 0.359958 6:+106860498 None:intergenic
AAGGGTCATTACTTCTGTTT+CGG 0.368590 6:-106860279 MsG0680035498.01.T01:CDS
TGCTTATTATCAATTGATCT+AGG 0.375333 6:+106860426 None:intergenic
TACTTCTGTTTCGGTGCACC+TGG 0.379873 6:-106860270 MsG0680035498.01.T01:CDS
ATCAATTGATAATAAGCAAA+AGG 0.410363 6:-106860421 MsG0680035498.01.T01:CDS
TTAAGAGAAATTCTGTTAGC+AGG 0.463517 6:-106860308 MsG0680035498.01.T01:CDS
TTCTGTTAGCAGGAAGTTGA+AGG 0.474185 6:-106860298 MsG0680035498.01.T01:CDS
TAAGCCACAGCAACAAAACT+AGG 0.476632 6:+106860219 None:intergenic
GATACTGACGATATTGTTGC+CGG 0.482463 6:-106860387 MsG0680035498.01.T01:CDS
TTGATTCAAGCAAGATGGTC+CGG 0.490864 6:+106860368 None:intergenic
TCAATTGATAATAAGCAAAA+GGG 0.495816 6:-106860420 MsG0680035498.01.T01:CDS
GGTGTCTCATCATCATCATA+AGG 0.533965 6:+106860339 None:intergenic
AGTTGTTGATTCAAGCAAGA+TGG 0.554714 6:+106860363 None:intergenic
TCTGTTAGCAGGAAGTTGAA+GGG 0.611806 6:-106860297 MsG0680035498.01.T01:CDS
GAAGTCTTCACATTAAGACC+AGG 0.700871 6:+106860252 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCAATTGATAATAAGCAAA+AGG - Chr6:106860412-106860431 MsG0680035498.01.T01:CDS 20.0%
!!! TCAATTGATAATAAGCAAAA+GGG - Chr6:106860413-106860432 MsG0680035498.01.T01:CDS 20.0%
! GAATTTCTCTTAACGAATTT+CGG + Chr6:106860518-106860537 None:intergenic 25.0%
! TGCTTATTATCAATTGATCT+AGG + Chr6:106860410-106860429 None:intergenic 25.0%
ACAAAAGAACACTTTCTGTT+AGG - Chr6:106860297-106860316 MsG0680035498.01.T01:CDS 30.0%
TTAAGAGAAATTCTGTTAGC+AGG - Chr6:106860525-106860544 MsG0680035498.01.T01:CDS 30.0%
! ACTTAGACAGAGATTTTCTT+CGG - Chr6:106860247-106860266 MsG0680035498.01.T01:CDS 30.0%
!!! GAAAGTGTTCTTTTGTTGTT+TGG + Chr6:106860293-106860312 None:intergenic 30.0%
AAGGGTCATTACTTCTGTTT+CGG - Chr6:106860554-106860573 MsG0680035498.01.T01:CDS 35.0%
GCAAATCTCTTCTTAGCATT+AGG + Chr6:106860338-106860357 None:intergenic 35.0%
!! AGTTGTTGATTCAAGCAAGA+TGG + Chr6:106860473-106860492 None:intergenic 35.0%
!!! TAAACCTAGTTTTGTTGCTG+TGG - Chr6:106860610-106860629 MsG0680035498.01.T01:CDS 35.0%
GAAGTCTTCACATTAAGACC+AGG + Chr6:106860584-106860603 None:intergenic 40.0%
GATACTGACGATATTGTTGC+CGG - Chr6:106860446-106860465 MsG0680035498.01.T01:CDS 40.0%
GGTGTCTCATCATCATCATA+AGG + Chr6:106860497-106860516 None:intergenic 40.0%
TAAGCCACAGCAACAAAACT+AGG + Chr6:106860617-106860636 None:intergenic 40.0%
TCTGTTAGCAGGAAGTTGAA+GGG - Chr6:106860536-106860555 MsG0680035498.01.T01:CDS 40.0%
TTCTGTTAGCAGGAAGTTGA+AGG - Chr6:106860535-106860554 MsG0680035498.01.T01:CDS 40.0%
TTGATTCAAGCAAGATGGTC+CGG + Chr6:106860468-106860487 None:intergenic 40.0%
! TACTTCTGTTTCGGTGCACC+TGG - Chr6:106860563-106860582 MsG0680035498.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 106860218 106860637 106860218 ID=MsG0680035498.01;Name=MsG0680035498.01
Chr6 mRNA 106860218 106860637 106860218 ID=MsG0680035498.01.T01;Parent=MsG0680035498.01;Name=MsG0680035498.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|139
Chr6 exon 106860218 106860637 106860218 ID=MsG0680035498.01.T01:exon:18829;Parent=MsG0680035498.01.T01
Chr6 CDS 106860218 106860637 106860218 ID=MsG0680035498.01.T01:cds;Parent=MsG0680035498.01.T01
Gene Sequence

>MsG0680035498.01.T01

ATGAGTACTAATAGTTTTGATGAAATTTCACTTAGACAGAGATTTTCTTCGGCTTCTAAGACGAATTTGATTCCAAACAACAAAAGAACACTTTCTGTTAGGAGCTATCAATCAAGTCCTAATGCTAAGAAGAGATTTGCATCAGCAAGAACAAGATCAAGTTCATTTTCAAAATCATCAATCTCTTCACCTAGATCAATTGATAATAAGCAAAAGGGTGTTTATATAGATACTGACGATATTGTTGCCGGACCATCTTGCTTGAATCAACAACTGCCTTATGATGATGATGAGACACCGAAATTCGTTAAGAGAAATTCTGTTAGCAGGAAGTTGAAGGGTCATTACTTCTGTTTCGGTGCACCTGGTCTTAATGTGAAGACTTCTGTTCATAAACCTAGTTTTGTTGCTGTGGCTTAA

Protein sequence

>MsG0680035498.01.T01

MSTNSFDEISLRQRFSSASKTNLIPNNKRTLSVRSYQSSPNAKKRFASARTRSSSFSKSSISSPRSIDNKQKGVYIDTDDIVAGPSCLNQQLPYDDDETPKFVKRNSVSRKLKGHYFCFGAPGLNVKTSVHKPSFVAVA*