Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036219.01.T01 | ADK12637.1 | 96.842 | 95 | 2 | 1 | 1 | 95 | 49 | 142 | 4.43E-56 | 185 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036219.01.T01 | O48782 | 73.958 | 96 | 24 | 1 | 4 | 98 | 50 | 145 | 2.20E-45 | 150 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036219.01.T01 | D9IWR7 | 96.842 | 95 | 2 | 1 | 1 | 95 | 49 | 142 | 2.12e-56 | 185 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000114.01 | MsG0780036219.01 | 0.813122 | 3.003176e-51 | 1.944241e-48 |
| MsG0180005397.01 | MsG0780036219.01 | 0.815012 | 1.149573e-51 | 7.834108e-49 |
| MsG0180006104.01 | MsG0780036219.01 | 0.808451 | 3.077299e-50 | 1.760214e-47 |
| MsG0180006243.01 | MsG0780036219.01 | 0.809109 | 2.225715e-50 | 1.295401e-47 |
| MsG0680034235.01 | MsG0780036219.01 | 0.856331 | 3.458726e-62 | 8.225773e-59 |
| MsG0480018847.01 | MsG0780036219.01 | 0.800855 | 1.184834e-48 | 5.575796e-46 |
| MsG0580024829.01 | MsG0780036219.01 | 0.803622 | 3.192314e-49 | 1.611831e-46 |
| MsG0380016740.01 | MsG0780036219.01 | 0.818472 | 1.926128e-52 | 1.443199e-49 |
| MsG0380017201.01 | MsG0780036219.01 | 0.810646 | 1.039345e-50 | 6.299953e-48 |
| MsG0380017401.01 | MsG0780036219.01 | 0.816185 | 6.301131e-52 | 4.434077e-49 |
| MsG0580028977.01 | MsG0780036219.01 | 0.803042 | 4.209526e-49 | 2.093944e-46 |
| MsG0580029647.01 | MsG0780036219.01 | 0.802990 | 4.315128e-49 | 2.143603e-46 |
| MsG0280007775.01 | MsG0780036219.01 | 0.826253 | 3.002083e-54 | 2.800635e-51 |
| MsG0280009350.01 | MsG0780036219.01 | 0.802300 | 5.990121e-49 | 2.924086e-46 |
| MsG0280010158.01 | MsG0780036219.01 | 0.829279 | 5.626906e-55 | 5.733204e-52 |
| MsG0780036117.01 | MsG0780036219.01 | 0.809167 | 2.162884e-50 | 1.260789e-47 |
| MsG0780036219.01 | MsG0780036430.01 | 0.804632 | 1.967693e-49 | 1.019614e-46 |
| MsG0780036219.01 | MsG0780036845.01 | 0.809658 | 1.697466e-50 | 1.002513e-47 |
| MsG0780036219.01 | MsG0780037399.01 | 0.805867 | 1.085139e-49 | 5.804728e-47 |
| MsG0780036219.01 | MsG0780041482.01 | 0.826492 | 2.632863e-54 | 2.472829e-51 |
| MsG0780036219.01 | MsG0880042033.01 | 0.800798 | 1.216934e-48 | 5.718326e-46 |
| MsG0780036219.01 | MsG0880047519.01 | 0.828196 | 1.028456e-54 | 1.015087e-51 |
| MsG0780036219.01 | MsG0880047696.01 | 0.817152 | 3.823474e-52 | 2.762791e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036219.01.T01 | MTR_8g019320 | 93.878 | 98 | 4 | 2 | 1 | 98 | 49 | 144 | 3.52e-58 | 180 |
| MsG0780036219.01.T01 | MTR_1g019970 | 43.023 | 86 | 43 | 2 | 18 | 97 | 69 | 154 | 6.16e-16 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036219.01.T01 | AT2G26670 | 73.958 | 96 | 24 | 1 | 4 | 98 | 50 | 145 | 2.24e-46 | 150 |
| MsG0780036219.01.T01 | AT1G69720 | 64.773 | 88 | 31 | 0 | 7 | 94 | 56 | 143 | 1.77e-37 | 126 |
| MsG0780036219.01.T01 | AT1G69720 | 63.441 | 93 | 33 | 1 | 7 | 98 | 56 | 148 | 6.66e-37 | 126 |
| MsG0780036219.01.T01 | AT1G69720 | 63.441 | 93 | 33 | 1 | 7 | 98 | 56 | 148 | 6.66e-37 | 126 |
| MsG0780036219.01.T01 | AT1G58300 | 47.191 | 89 | 42 | 2 | 5 | 92 | 55 | 139 | 3.15e-24 | 93.6 |
| MsG0780036219.01.T01 | AT2G26670 | 81.818 | 44 | 8 | 0 | 4 | 47 | 50 | 93 | 1.44e-18 | 77.8 |
| MsG0780036219.01.T01 | AT2G26550 | 37.755 | 98 | 43 | 2 | 18 | 97 | 66 | 163 | 5.09e-13 | 63.5 |
| MsG0780036219.01.T01 | AT2G26550 | 35.897 | 117 | 53 | 3 | 3 | 97 | 47 | 163 | 6.15e-13 | 63.5 |
| MsG0780036219.01.T01 | AT2G26550 | 35.897 | 117 | 53 | 3 | 3 | 97 | 47 | 163 | 6.33e-13 | 63.2 |
| MsG0780036219.01.T01 | AT2G26550 | 37.755 | 98 | 43 | 2 | 18 | 97 | 66 | 163 | 9.38e-13 | 63.2 |
Find 27 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAACCGTCGATTGTTTCAT+AGG | 0.369189 | 7:-4823352 | None:intergenic |
| TATAAAGAAAACTTACAATA+AGG | 0.386222 | 7:-4823634 | None:intergenic |
| CTTTCTCGCCTTCTTTCGCC+TGG | 0.421199 | 7:-4823483 | None:intergenic |
| TGTGGAGGAGATGAGGTTTG+TGG | 0.450912 | 7:+4823438 | MsG0780036219.01.T01:CDS |
| AAGTGGGAACCTAGTGTTGA+TGG | 0.451461 | 7:+4823538 | MsG0780036219.01.T01:CDS |
| AGATGAGGTTTGTGGCTATG+AGG | 0.468777 | 7:+4823446 | MsG0780036219.01.T01:CDS |
| AGGTGAGTCAAAGGGGTTTG+TGG | 0.473420 | 7:+4823420 | MsG0780036219.01.T01:CDS |
| ACAAACCCCTTTGACTCACC+TGG | 0.498355 | 7:-4823418 | None:intergenic |
| GTCGGCGACGTCGGCAGCGG+CGG | 0.508037 | 7:+4823378 | MsG0780036219.01.T01:CDS |
| CGTCATCCAGGTGAGTCAAA+GGG | 0.514210 | 7:+4823412 | MsG0780036219.01.T01:CDS |
| ACAAAGGACCAGGCGAAAGA+AGG | 0.517835 | 7:+4823475 | MsG0780036219.01.T01:CDS |
| GAGAAGAAGAAGCGTCATCC+AGG | 0.520487 | 7:+4823400 | MsG0780036219.01.T01:CDS |
| TGTGTCGGCGACGTCGGCAG+CGG | 0.520703 | 7:+4823375 | MsG0780036219.01.T01:CDS |
| GGTTATTGTGTCGGCGACGT+CGG | 0.523141 | 7:+4823369 | MsG0780036219.01.T01:CDS |
| CTAGTGTTGATGGGTATTTG+AGG | 0.525368 | 7:+4823548 | MsG0780036219.01.T01:CDS |
| GCGTCATCCAGGTGAGTCAA+AGG | 0.526306 | 7:+4823411 | MsG0780036219.01.T01:CDS |
| AGGGGTTTGTGGAGGAGATG+AGG | 0.527030 | 7:+4823431 | MsG0780036219.01.T01:CDS |
| CTCAAATACCCATCAACACT+AGG | 0.558203 | 7:-4823547 | None:intergenic |
| GGCTATGAGGTTACATACAA+AGG | 0.594106 | 7:+4823459 | MsG0780036219.01.T01:CDS |
| AAGAGAGAGCTGTCACAAAG+TGG | 0.607127 | 7:+4823521 | MsG0780036219.01.T01:CDS |
| AGTGGGAACCTAGTGTTGAT+GGG | 0.619249 | 7:+4823539 | MsG0780036219.01.T01:CDS |
| AGAGAGAGCTGTCACAAAGT+GGG | 0.634592 | 7:+4823522 | MsG0780036219.01.T01:CDS |
| TGAGTCAAAGGGGTTTGTGG+AGG | 0.635632 | 7:+4823423 | MsG0780036219.01.T01:CDS |
| ACAATCGACGGTTATTGTGT+CGG | 0.635834 | 7:+4823360 | MsG0780036219.01.T01:CDS |
| GTCATCCAGGTGAGTCAAAG+GGG | 0.646453 | 7:+4823413 | MsG0780036219.01.T01:CDS |
| CTCTCCTATGAAACAATCGA+CGG | 0.650681 | 7:+4823348 | None:intergenic |
| GAGGTTACATACAAAGGACC+AGG | 0.660350 | 7:+4823465 | MsG0780036219.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAGAAAACTTACAATA+AGG | - | Chr7:4823637-4823656 | None:intergenic | 15.0% |
| ACAATCGACGGTTATTGTGT+CGG | + | Chr7:4823360-4823379 | MsG0780036219.01.T01:CDS | 40.0% | |
| CTCAAATACCCATCAACACT+AGG | - | Chr7:4823550-4823569 | None:intergenic | 40.0% | |
| GGCTATGAGGTTACATACAA+AGG | + | Chr7:4823459-4823478 | MsG0780036219.01.T01:CDS | 40.0% | |
| !! | CTAGTGTTGATGGGTATTTG+AGG | + | Chr7:4823548-4823567 | MsG0780036219.01.T01:CDS | 40.0% |
| AAGAGAGAGCTGTCACAAAG+TGG | + | Chr7:4823521-4823540 | MsG0780036219.01.T01:CDS | 45.0% | |
| AGAGAGAGCTGTCACAAAGT+GGG | + | Chr7:4823522-4823541 | MsG0780036219.01.T01:CDS | 45.0% | |
| AGATGAGGTTTGTGGCTATG+AGG | + | Chr7:4823446-4823465 | MsG0780036219.01.T01:CDS | 45.0% | |
| GAGGTTACATACAAAGGACC+AGG | + | Chr7:4823465-4823484 | MsG0780036219.01.T01:CDS | 45.0% | |
| ! | AAGTGGGAACCTAGTGTTGA+TGG | + | Chr7:4823538-4823557 | MsG0780036219.01.T01:CDS | 45.0% |
| !! | AGTGGGAACCTAGTGTTGAT+GGG | + | Chr7:4823539-4823558 | MsG0780036219.01.T01:CDS | 45.0% |
| ACAAACCCCTTTGACTCACC+TGG | - | Chr7:4823421-4823440 | None:intergenic | 50.0% | |
| ACAAAGGACCAGGCGAAAGA+AGG | + | Chr7:4823475-4823494 | MsG0780036219.01.T01:CDS | 50.0% | |
| AGGTGAGTCAAAGGGGTTTG+TGG | + | Chr7:4823420-4823439 | MsG0780036219.01.T01:CDS | 50.0% | |
| CGTCATCCAGGTGAGTCAAA+GGG | + | Chr7:4823412-4823431 | MsG0780036219.01.T01:CDS | 50.0% | |
| GTCATCCAGGTGAGTCAAAG+GGG | + | Chr7:4823413-4823432 | MsG0780036219.01.T01:CDS | 50.0% | |
| TGAGTCAAAGGGGTTTGTGG+AGG | + | Chr7:4823423-4823442 | MsG0780036219.01.T01:CDS | 50.0% | |
| TGTGGAGGAGATGAGGTTTG+TGG | + | Chr7:4823438-4823457 | MsG0780036219.01.T01:CDS | 50.0% | |
| !! | GAGAAGAAGAAGCGTCATCC+AGG | + | Chr7:4823400-4823419 | MsG0780036219.01.T01:CDS | 50.0% |
| AGGGGTTTGTGGAGGAGATG+AGG | + | Chr7:4823431-4823450 | MsG0780036219.01.T01:CDS | 55.0% | |
| CTTTCTCGCCTTCTTTCGCC+TGG | - | Chr7:4823486-4823505 | None:intergenic | 55.0% | |
| GCGTCATCCAGGTGAGTCAA+AGG | + | Chr7:4823411-4823430 | MsG0780036219.01.T01:CDS | 55.0% | |
| ! | GGTTATTGTGTCGGCGACGT+CGG | + | Chr7:4823369-4823388 | MsG0780036219.01.T01:CDS | 55.0% |
| ! | TGTGTCGGCGACGTCGGCAG+CGG | + | Chr7:4823375-4823394 | MsG0780036219.01.T01:CDS | 70.0% |
| ! | GTCGGCGACGTCGGCAGCGG+CGG | + | Chr7:4823378-4823397 | MsG0780036219.01.T01:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 4823355 | 4823675 | 4823355 | ID=MsG0780036219.01;Name=MsG0780036219.01 |
| Chr7 | mRNA | 4823355 | 4823675 | 4823355 | ID=MsG0780036219.01.T01;Parent=MsG0780036219.01;Name=MsG0780036219.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|106 |
| Chr7 | exon | 4823355 | 4823675 | 4823355 | ID=MsG0780036219.01.T01:exon:2708;Parent=MsG0780036219.01.T01 |
| Chr7 | CDS | 4823355 | 4823675 | 4823355 | ID=MsG0780036219.01.T01:cds;Parent=MsG0780036219.01.T01 |
| Gene Sequence |
| Protein sequence |
