AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018847.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018847.01.T01 MTR_4g019130 97.512 201 5 0 1 201 1 201 3.13e-140 389
MsG0480018847.01.T01 MTR_4g019150 73.301 206 46 3 1 201 1 202 2.26e-104 299
MsG0480018847.01.T01 MTR_7g104650 37.000 200 108 10 11 201 102 292 2.33e-23 94.7
MsG0480018847.01.T01 MTR_2g054130 36.979 192 95 5 11 201 211 377 3.60e-22 92.8
MsG0480018847.01.T01 MTR_2g055010 37.079 178 86 5 11 187 211 363 1.70e-19 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018847.01.T01 AT2G37970 60.648 216 69 3 1 201 32 246 1.23e-88 261
MsG0480018847.01.T01 AT5G20140 34.031 191 103 5 11 201 206 373 2.69e-21 90.5
MsG0480018847.01.T01 AT3G10130 37.000 200 108 10 11 201 115 305 3.37e-19 84.0
MsG0480018847.01.T01 AT5G20140 33.898 177 94 5 11 187 206 359 8.36e-19 83.6

Find 38 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACAAAGATGGTGGGTTTA+AGG 0.186605 4:+10566152 MsG0480018847.01.T01:CDS
GAGGAAGGTGGGAAGAAATA+TGG 0.218011 4:+10566414 MsG0480018847.01.T01:CDS
TTTGATATGGGATTAGTATT+TGG 0.244674 4:+10566009 None:intergenic
AAATATGGGGTGGTGACTTT+TGG 0.302611 4:+10566429 MsG0480018847.01.T01:CDS
TTCTTCTCATTTGTTACAAC+TGG 0.303094 4:-10566300 None:intergenic
CGATTACAATTCCAATGTTT+AGG 0.308020 4:+10566568 MsG0480018847.01.T01:CDS
TTGTCACCTTTGAATGTTGA+TGG 0.319242 4:-10566135 None:intergenic
AGGAAGGTGGGAAGAAATAT+GGG 0.347201 4:+10566415 MsG0480018847.01.T01:CDS
ATCATCATGGTTCATTCAAC+AGG 0.393338 4:-10566609 None:intergenic
GGAGAAGTTGAGGTTGTGTT+TGG 0.422907 4:+10566488 MsG0480018847.01.T01:CDS
TTTGAATGTTGATGGATCAT+AGG 0.426497 4:-10566127 None:intergenic
ATCACAACCCTCTCATCAAT+AGG 0.466452 4:-10566387 None:intergenic
TTGTGATAAGAGAGGAAGGT+GGG 0.471919 4:+10566403 MsG0480018847.01.T01:CDS
AGGGTTGTGATAAGAGAGGA+AGG 0.486753 4:+10566399 MsG0480018847.01.T01:CDS
AGGAGAAGGTGGAGAAGTTG+AGG 0.492065 4:+10566478 MsG0480018847.01.T01:CDS
TCCAATGTTTAGGACTAATG+AGG 0.513944 4:+10566578 MsG0480018847.01.T01:CDS
AGGTTGTGTTTGGAGAAAGA+TGG 0.524733 4:+10566498 MsG0480018847.01.T01:CDS
TGAGAGGGTTGTGATAAGAG+AGG 0.529346 4:+10566395 MsG0480018847.01.T01:CDS
GGCAAGTGATGAAGTGGTGA+AGG 0.533494 4:+10566458 MsG0480018847.01.T01:CDS
TATGATCCATCAACATTCAA+AGG 0.542632 4:+10566129 MsG0480018847.01.T01:CDS
AACATTGGAATTGTAATCGC+AGG 0.545246 4:-10566564 None:intergenic
ACCTAAGCCTATTGATGAGA+GGG 0.548573 4:+10566380 MsG0480018847.01.T01:CDS
TGAAGTGGTGAAGGAGAAGG+TGG 0.549018 4:+10566467 MsG0480018847.01.T01:CDS
ACCCTCTCATCAATAGGCTT+AGG 0.549333 4:-10566381 None:intergenic
ACCTCATTAGTCCTAAACAT+TGG 0.561532 4:-10566579 None:intergenic
ATTGGAATTGTAATCGCAGG+TGG 0.568955 4:-10566561 None:intergenic
TGATGAAGTGGTGAAGGAGA+AGG 0.572950 4:+10566464 MsG0480018847.01.T01:CDS
ACATTCAAAGGTGACAAAGA+TGG 0.580055 4:+10566141 MsG0480018847.01.T01:CDS
TTCAAAGGTGACAAAGATGG+TGG 0.592548 4:+10566144 MsG0480018847.01.T01:CDS
GTGATTTCAGCTACTAGAGA+AGG 0.604224 4:-10566105 None:intergenic
TACCTAAGCCTATTGATGAG+AGG 0.615966 4:+10566379 MsG0480018847.01.T01:CDS
AGGAGTGGCAAGTGATGAAG+TGG 0.624838 4:+10566452 MsG0480018847.01.T01:CDS
AACAAATGAGAAGAACAAGA+TGG 0.636525 4:+10566308 MsG0480018847.01.T01:CDS
TCAAAGGTGACAAAGATGGT+GGG 0.643345 4:+10566145 MsG0480018847.01.T01:CDS
AGGTGGGAAGAAATATGGGG+TGG 0.665542 4:+10566419 MsG0480018847.01.T01:CDS
GTTGTGATAAGAGAGGAAGG+TGG 0.671478 4:+10566402 MsG0480018847.01.T01:CDS
GGAAGGTGGGAAGAAATATG+GGG 0.684324 4:+10566416 MsG0480018847.01.T01:CDS
GACTTCACCAGTTATCACAA+AGG 0.689440 4:+10566239 MsG0480018847.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTGAGTATTTCATATTT+TGG - Chr4:10566054-10566073 None:intergenic 20.0%
! TTCTATGTATGAAAAAGTAG+AGG + Chr4:10566353-10566372 MsG0480018847.01.T01:CDS 25.0%
!! GTTGATTACATAGGAATTTT+TGG + Chr4:10566180-10566199 MsG0480018847.01.T01:CDS 25.0%
!!! AAAGATGGTTTTAAGGTTAT+TGG + Chr4:10566513-10566532 MsG0480018847.01.T01:CDS 25.0%
!!! AAGGTTTTAGTTGATTACAT+AGG + Chr4:10566171-10566190 MsG0480018847.01.T01:CDS 25.0%
!!! GTTATTGGAGATTTTTTGTT+GGG + Chr4:10566528-10566547 MsG0480018847.01.T01:CDS 25.0%
!!! TCTACTTTTTCATACATAGA+AGG - Chr4:10566354-10566373 None:intergenic 25.0%
!!! TTTTGTGTTTTTGTGTTTTG+AGG - Chr4:10566210-10566229 None:intergenic 25.0%
AACAAATGAGAAGAACAAGA+TGG + Chr4:10566308-10566327 MsG0480018847.01.T01:CDS 30.0%
CGATTACAATTCCAATGTTT+AGG + Chr4:10566568-10566587 MsG0480018847.01.T01:CDS 30.0%
TATGATCCATCAACATTCAA+AGG + Chr4:10566129-10566148 MsG0480018847.01.T01:CDS 30.0%
! TTCTTCTCATTTGTTACAAC+TGG - Chr4:10566303-10566322 None:intergenic 30.0%
! TTGTTTTCCTTTGTGATAAC+TGG - Chr4:10566249-10566268 None:intergenic 30.0%
! TTTGAATGTTGATGGATCAT+AGG - Chr4:10566130-10566149 None:intergenic 30.0%
!! TTTGGAGAAAGATGGTTTTA+AGG + Chr4:10566506-10566525 MsG0480018847.01.T01:CDS 30.0%
!!! GGTTATTGGAGATTTTTTGT+TGG + Chr4:10566527-10566546 MsG0480018847.01.T01:CDS 30.0%
AACATTGGAATTGTAATCGC+AGG - Chr4:10566567-10566586 None:intergenic 35.0%
ACATTCAAAGGTGACAAAGA+TGG + Chr4:10566141-10566160 MsG0480018847.01.T01:CDS 35.0%
ACCTCATTAGTCCTAAACAT+TGG - Chr4:10566582-10566601 None:intergenic 35.0%
TTGTCACCTTTGAATGTTGA+TGG - Chr4:10566138-10566157 None:intergenic 35.0%
! TCCAATGTTTAGGACTAATG+AGG + Chr4:10566578-10566597 MsG0480018847.01.T01:CDS 35.0%
!! CTAGAGAAGGTGCATATTTT+CGG - Chr4:10566095-10566114 None:intergenic 35.0%
!!! TCGGATTACATAGTTTTGTG+TGG - Chr4:10566076-10566095 None:intergenic 35.0%
!!! TTGGAGATTTTTTGTTGGGT+AGG + Chr4:10566532-10566551 MsG0480018847.01.T01:CDS 35.0%
ACCTAAGCCTATTGATGAGA+GGG + Chr4:10566380-10566399 MsG0480018847.01.T01:CDS 40.0%
AGGAAGGTGGGAAGAAATAT+GGG + Chr4:10566415-10566434 MsG0480018847.01.T01:CDS 40.0%
AGGTTGTGTTTGGAGAAAGA+TGG + Chr4:10566498-10566517 MsG0480018847.01.T01:CDS 40.0%
ATCACAACCCTCTCATCAAT+AGG - Chr4:10566390-10566409 None:intergenic 40.0%
ATTGGAATTGTAATCGCAGG+TGG - Chr4:10566564-10566583 None:intergenic 40.0%
GACTTCACCAGTTATCACAA+AGG + Chr4:10566239-10566258 MsG0480018847.01.T01:CDS 40.0%
GTGATTTCAGCTACTAGAGA+AGG - Chr4:10566108-10566127 None:intergenic 40.0%
TACCTAAGCCTATTGATGAG+AGG + Chr4:10566379-10566398 MsG0480018847.01.T01:CDS 40.0%
TCAAAGGTGACAAAGATGGT+GGG + Chr4:10566145-10566164 MsG0480018847.01.T01:CDS 40.0%
TTCAAAGGTGACAAAGATGG+TGG + Chr4:10566144-10566163 MsG0480018847.01.T01:CDS 40.0%
TTGTGATAAGAGAGGAAGGT+GGG + Chr4:10566403-10566422 MsG0480018847.01.T01:CDS 40.0%
! AAATATGGGGTGGTGACTTT+TGG + Chr4:10566429-10566448 MsG0480018847.01.T01:CDS 40.0%
!! TGACAAAGATGGTGGGTTTA+AGG + Chr4:10566152-10566171 MsG0480018847.01.T01:CDS 40.0%
ACCCTCTCATCAATAGGCTT+AGG - Chr4:10566384-10566403 None:intergenic 45.0%
AGGGTTGTGATAAGAGAGGA+AGG + Chr4:10566399-10566418 MsG0480018847.01.T01:CDS 45.0%
GAGGAAGGTGGGAAGAAATA+TGG + Chr4:10566414-10566433 MsG0480018847.01.T01:CDS 45.0%
GGAAGGTGGGAAGAAATATG+GGG + Chr4:10566416-10566435 MsG0480018847.01.T01:CDS 45.0%
GGAGAAGTTGAGGTTGTGTT+TGG + Chr4:10566488-10566507 MsG0480018847.01.T01:CDS 45.0%
GTTGTGATAAGAGAGGAAGG+TGG + Chr4:10566402-10566421 MsG0480018847.01.T01:CDS 45.0%
TGAGAGGGTTGTGATAAGAG+AGG + Chr4:10566395-10566414 MsG0480018847.01.T01:CDS 45.0%
TGATGAAGTGGTGAAGGAGA+AGG + Chr4:10566464-10566483 MsG0480018847.01.T01:CDS 45.0%
AGGAGAAGGTGGAGAAGTTG+AGG + Chr4:10566478-10566497 MsG0480018847.01.T01:CDS 50.0%
AGGAGTGGCAAGTGATGAAG+TGG + Chr4:10566452-10566471 MsG0480018847.01.T01:CDS 50.0%
AGGTGGGAAGAAATATGGGG+TGG + Chr4:10566419-10566438 MsG0480018847.01.T01:CDS 50.0%
GGCAAGTGATGAAGTGGTGA+AGG + Chr4:10566458-10566477 MsG0480018847.01.T01:CDS 50.0%
TGAAGTGGTGAAGGAGAAGG+TGG + Chr4:10566467-10566486 MsG0480018847.01.T01:CDS 50.0%
! TATGGGGTGGTGACTTTTGG+AGG + Chr4:10566432-10566451 MsG0480018847.01.T01:CDS 50.0%
!! GGTGGTGACTTTTGGAGGAG+TGG + Chr4:10566437-10566456 MsG0480018847.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 10566015 10566620 10566015 ID=MsG0480018847.01;Name=MsG0480018847.01
Chr4 mRNA 10566015 10566620 10566015 ID=MsG0480018847.01.T01;Parent=MsG0480018847.01;Name=MsG0480018847.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|201
Chr4 exon 10566015 10566620 10566015 ID=MsG0480018847.01.T01:exon:3041;Parent=MsG0480018847.01.T01
Chr4 CDS 10566015 10566620 10566015 ID=MsG0480018847.01.T01:cds;Parent=MsG0480018847.01.T01
Gene Sequence

>MsG0480018847.01.T01

ATGGGATTAGTATTTGGAAGATTTAGTGCTGAGACACCAAAATATGAAATACTCAAAACCACACAAAACTATGTAATCCGAAAATATGCACCTTCTCTAGTAGCTGAAATCACCTATGATCCATCAACATTCAAAGGTGACAAAGATGGTGGGTTTAAGGTTTTAGTTGATTACATAGGAATTTTTGGTAAACCTCAAAACACAAAAACACAAAAAATCTCAATGACTTCACCAGTTATCACAAAGGAAAACAAAATTTTTTCAGAAAAGATTGCTATGACAGTACCAGTTGTAACAAATGAGAAGAACAAGATGGTGACTATGCAGTTTACTTTGCCTTCTATGTATGAAAAAGTAGAGGAAGTACCTAAGCCTATTGATGAGAGGGTTGTGATAAGAGAGGAAGGTGGGAAGAAATATGGGGTGGTGACTTTTGGAGGAGTGGCAAGTGATGAAGTGGTGAAGGAGAAGGTGGAGAAGTTGAGGTTGTGTTTGGAGAAAGATGGTTTTAAGGTTATTGGAGATTTTTTGTTGGGTAGGTATAATCCACCTGCGATTACAATTCCAATGTTTAGGACTAATGAGGTTTTGATTCCTGTTGAATGA

Protein sequence

>MsG0480018847.01.T01

MGLVFGRFSAETPKYEILKTTQNYVIRKYAPSLVAEITYDPSTFKGDKDGGFKVLVDYIGIFGKPQNTKTQKISMTSPVITKENKIFSEKIAMTVPVVTNEKNKMVTMQFTLPSMYEKVEEVPKPIDERVVIREEGGKKYGVVTFGGVASDEVVKEKVEKLRLCLEKDGFKVIGDFLLGRYNPPAITIPMFRTNEVLIPVE*