AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180006243.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006243.01.T01 MTR_1g116590 86.719 128 3 5 1 126 1 116 8.93e-70 205
MsG0180006243.01.T01 MTR_1g080750 43.519 108 36 2 40 122 65 172 1.62e-22 87.4
MsG0180006243.01.T01 MTR_7g115290 40.385 104 34 2 47 122 79 182 8.32e-20 80.5
MsG0180006243.01.T01 MTR_5g017450 39.655 116 35 5 41 123 57 170 4.78e-15 67.8
MsG0180006243.01.T01 MTR_7g110880 50.704 71 31 2 49 119 214 280 1.26e-14 68.6
MsG0180006243.01.T01 MTR_4g085970 38.000 100 44 3 42 123 50 149 3.91e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006243.01.T01 AT2G31560 61.594 138 30 1 12 126 65 202 3.02e-53 166
MsG0180006243.01.T01 AT2G31560 61.594 138 30 1 12 126 65 202 3.02e-53 166
MsG0180006243.01.T01 AT2G31560 61.594 138 30 1 12 126 65 202 3.02e-53 166
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT1G05870 60.403 149 26 4 10 126 42 189 2.06e-52 164
MsG0180006243.01.T01 AT2G43340 57.718 149 35 3 3 126 44 189 2.68e-47 150
MsG0180006243.01.T01 AT3G22690 61.728 81 23 1 45 117 843 923 1.86e-24 97.4
MsG0180006243.01.T01 AT3G04700 39.316 117 37 2 37 122 74 187 3.63e-19 79.0
MsG0180006243.01.T01 AT5G28690 37.903 124 44 3 29 122 68 188 8.51e-19 78.2
MsG0180006243.01.T01 AT5G28690 37.903 124 44 3 29 122 68 188 8.51e-19 78.2
MsG0180006243.01.T01 AT5G28690 37.903 124 44 3 29 122 68 188 8.51e-19 78.2
MsG0180006243.01.T01 AT1G08790 40.741 108 34 3 45 122 78 185 1.10e-18 77.8
MsG0180006243.01.T01 AT3G50350 42.056 107 43 2 35 122 59 165 1.71e-18 77.0
MsG0180006243.01.T01 AT3G04710 38.462 117 38 2 37 122 74 187 2.60e-18 80.1
MsG0180006243.01.T01 AT4G33985 45.370 108 39 2 39 126 43 150 3.17e-18 75.9
MsG0180006243.01.T01 AT2G15590 38.318 107 47 3 39 126 40 146 7.75e-13 61.6
MsG0180006243.01.T01 AT3G50350 51.613 62 28 1 35 94 63 124 1.61e-12 61.6
MsG0180006243.01.T01 AT3G62070 46.667 75 30 3 50 119 150 219 3.06e-12 61.6
MsG0180006243.01.T01 AT2G46940 47.143 70 29 3 50 119 185 246 1.28e-11 60.1
MsG0180006243.01.T01 AT4G01670 49.296 71 30 2 49 119 172 236 8.55e-11 57.8

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATGTTTGGATCTTGGTTT+TGG 0.184629 1:-101791981 MsG0180006243.01.T01:CDS
TTAAAGCAAGATTGAAATAT+TGG 0.250359 1:-101791824 MsG0180006243.01.T01:CDS
AATCTTGCTTTAACATCTTC+AGG 0.331635 1:+101791834 None:intergenic
TTCCAATTGGCAAATGAATC+AGG 0.369053 1:+101791879 None:intergenic
CTCCTGATTCATTTGCCAAT+TGG 0.369305 1:-101791881 MsG0180006243.01.T01:CDS
TGATGAGTTGAAGGGATGTT+TGG 0.374718 1:-101791994 MsG0180006243.01.T01:CDS
TAAAGCAAGATTGAAATATT+GGG 0.375422 1:-101791823 MsG0180006243.01.T01:CDS
TTGAAGGGATGTTTGGATCT+TGG 0.389102 1:-101791987 MsG0180006243.01.T01:CDS
TAGTTAGCTGCAGAGTTTAA+CGG 0.390779 1:+101791782 None:intergenic
GGGAGGGTGTTACATAGTTC+AGG 0.412792 1:+101791939 None:intergenic
ATGGAAGGTGGAGGTGTTGT+TGG 0.413737 1:-101792143 MsG0180006243.01.T01:CDS
CATCAAGATCCTCATCTGTT+AGG 0.424926 1:+101792012 None:intergenic
TGGACTTGAGATCTTCCAAT+TGG 0.470698 1:+101791866 None:intergenic
GGAGGTGTTGTTGGAAGAAG+TGG 0.473169 1:-101792134 MsG0180006243.01.T01:CDS
TGAGGATCTTGATGAGTTGA+AGG 0.481670 1:-101792003 MsG0180006243.01.T01:CDS
TAACAAAGGAGTAGTGTTGT+TGG 0.483852 1:-101792081 MsG0180006243.01.T01:CDS
GAATAACACAACTCTAGAGC+TGG 0.494397 1:+101791918 None:intergenic
ATAATGAGGAGGATCTTTGT+AGG 0.495986 1:-101792043 MsG0180006243.01.T01:CDS
AAGGAGTAGTGTTGTTGGAA+GGG 0.511274 1:-101792076 MsG0180006243.01.T01:CDS
AAAGGAGTAGTGTTGTTGGA+AGG 0.535317 1:-101792077 MsG0180006243.01.T01:CDS
ACATCTTCAGGATGATCACC+TGG 0.572260 1:+101791846 None:intergenic
AGGGTATGTTGATGATAATG+AGG 0.584846 1:-101792057 MsG0180006243.01.T01:CDS
AATAACACAACTCTAGAGCT+GGG 0.590951 1:+101791919 None:intergenic
AATTGGAAGATCTCAAGTCC+AGG 0.596552 1:-101791864 MsG0180006243.01.T01:CDS
GTTGTTGGAAGAAGTGGAAG+TGG 0.596897 1:-101792128 MsG0180006243.01.T01:CDS
ACACAACTCTAGAGCTGGGA+GGG 0.600482 1:+101791923 None:intergenic
GACGAAAAGCCTAACAGATG+AGG 0.616832 1:-101792021 MsG0180006243.01.T01:CDS
TTGTTGGAAGAAGTGGAAGT+GGG 0.619030 1:-101792127 MsG0180006243.01.T01:CDS
AGTAAGAATAACAATAACAA+AGG 0.625105 1:-101792095 MsG0180006243.01.T01:CDS
GAGGATCTTGATGAGTTGAA+GGG 0.630888 1:-101792002 MsG0180006243.01.T01:CDS
GTATGTTGATGATAATGAGG+AGG 0.675262 1:-101792054 MsG0180006243.01.T01:CDS
AACACAACTCTAGAGCTGGG+AGG 0.715725 1:+101791922 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAAGAATAACAATAACAA+AGG - Chr1:101791833-101791852 MsG0180006243.01.T01:CDS 20.0%
!! TAAAGCAAGATTGAAATATT+GGG - Chr1:101792105-101792124 MsG0180006243.01.T01:CDS 20.0%
!! TTAAAGCAAGATTGAAATAT+TGG - Chr1:101792104-101792123 MsG0180006243.01.T01:CDS 20.0%
AATCTTGCTTTAACATCTTC+AGG + Chr1:101792097-101792116 None:intergenic 30.0%
ATAATGAGGAGGATCTTTGT+AGG - Chr1:101791885-101791904 MsG0180006243.01.T01:CDS 35.0%
TAACAAAGGAGTAGTGTTGT+TGG - Chr1:101791847-101791866 MsG0180006243.01.T01:CDS 35.0%
TTCCAATTGGCAAATGAATC+AGG + Chr1:101792052-101792071 None:intergenic 35.0%
!! AATAACACAACTCTAGAGCT+GGG + Chr1:101792012-101792031 None:intergenic 35.0%
!! AGGGTATGTTGATGATAATG+AGG - Chr1:101791871-101791890 MsG0180006243.01.T01:CDS 35.0%
!! GATGTTTGGATCTTGGTTTT+GGG - Chr1:101791948-101791967 MsG0180006243.01.T01:CDS 35.0%
!! GTATGTTGATGATAATGAGG+AGG - Chr1:101791874-101791893 MsG0180006243.01.T01:CDS 35.0%
AATTGGAAGATCTCAAGTCC+AGG - Chr1:101792064-101792083 MsG0180006243.01.T01:CDS 40.0%
CATCAAGATCCTCATCTGTT+AGG + Chr1:101791919-101791938 None:intergenic 40.0%
CTCCTGATTCATTTGCCAAT+TGG - Chr1:101792047-101792066 MsG0180006243.01.T01:CDS 40.0%
TGGACTTGAGATCTTCCAAT+TGG + Chr1:101792065-101792084 None:intergenic 40.0%
TTGAAGGGATGTTTGGATCT+TGG - Chr1:101791941-101791960 MsG0180006243.01.T01:CDS 40.0%
TTGTTGGAAGAAGTGGAAGT+GGG - Chr1:101791801-101791820 MsG0180006243.01.T01:CDS 40.0%
! AAAGGAGTAGTGTTGTTGGA+AGG - Chr1:101791851-101791870 MsG0180006243.01.T01:CDS 40.0%
! AAGGAGTAGTGTTGTTGGAA+GGG - Chr1:101791852-101791871 MsG0180006243.01.T01:CDS 40.0%
! GAGGATCTTGATGAGTTGAA+GGG - Chr1:101791926-101791945 MsG0180006243.01.T01:CDS 40.0%
! GGATGTTTGGATCTTGGTTT+TGG - Chr1:101791947-101791966 MsG0180006243.01.T01:CDS 40.0%
! TGAGGATCTTGATGAGTTGA+AGG - Chr1:101791925-101791944 MsG0180006243.01.T01:CDS 40.0%
! TGATGAGTTGAAGGGATGTT+TGG - Chr1:101791934-101791953 MsG0180006243.01.T01:CDS 40.0%
!! GAATAACACAACTCTAGAGC+TGG + Chr1:101792013-101792032 None:intergenic 40.0%
ACATCTTCAGGATGATCACC+TGG + Chr1:101792085-101792104 None:intergenic 45.0%
GACGAAAAGCCTAACAGATG+AGG - Chr1:101791907-101791926 MsG0180006243.01.T01:CDS 45.0%
GTTGTTGGAAGAAGTGGAAG+TGG - Chr1:101791800-101791819 MsG0180006243.01.T01:CDS 45.0%
!! AACACAACTCTAGAGCTGGG+AGG + Chr1:101792009-101792028 None:intergenic 50.0%
!! ACACAACTCTAGAGCTGGGA+GGG + Chr1:101792008-101792027 None:intergenic 50.0%
!! ATGGAAGGTGGAGGTGTTGT+TGG - Chr1:101791785-101791804 MsG0180006243.01.T01:CDS 50.0%
!! GGAGGTGTTGTTGGAAGAAG+TGG - Chr1:101791794-101791813 MsG0180006243.01.T01:CDS 50.0%
!! GGGAGGGTGTTACATAGTTC+AGG + Chr1:101791992-101792011 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 101791785 101792165 101791785 ID=MsG0180006243.01;Name=MsG0180006243.01
Chr1 mRNA 101791785 101792165 101791785 ID=MsG0180006243.01.T01;Parent=MsG0180006243.01;Name=MsG0180006243.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|126
Chr1 exon 101791785 101792165 101791785 ID=MsG0180006243.01.T01:exon:51919;Parent=MsG0180006243.01.T01
Chr1 CDS 101791785 101792165 101791785 ID=MsG0180006243.01.T01:cds;Parent=MsG0180006243.01.T01
Gene Sequence

>MsG0180006243.01.T01

ATGGAAGGTGGAGGTGTTGTTGGAAGAAGTGGAAGTGGGAAGATGAAGAGTAAGAATAACAATAACAAAGGAGTAGTGTTGTTGGAAGGGTATGTTGATGATAATGAGGAGGATCTTTGTAGGACGAAAAGCCTAACAGATGAGGATCTTGATGAGTTGAAGGGATGTTTGGATCTTGGTTTTGGGTTCAGTTATGATGAGATTCCTGAACTATGTAACACCCTCCCAGCTCTAGAGTTGTGTTATTCAATGTCAAACACTTCTCCTGATTCATTTGCCAATTGGAAGATCTCAAGTCCAGGTGATCATCCTGAAGATGTTAAAGCAAGATTGAAATATTGGGCACAAGCTGTTGCGTGTACCGTTAAACTCTGCAGCTAA

Protein sequence

>MsG0180006243.01.T01

MEGGGVVGRSGSGKMKSKNNNNKGVVLLEGYVDDNEEDLCRTKSLTDEDLDELKGCLDLGFGFSYDEIPELCNTLPALELCYSMSNTSPDSFANWKISSPGDHPEDVKARLKYWAQAVACTVKLCS*